Levels Of NDF Research Articles

EFFECT OF UREA AND/OR CRUDE GLYCERIN TREATMENT ON THE NUTRIENT COMPOSITION AND IN VITRO TRUE DIGESTIBILITY OF DIFFERENT VARIETIES OF RICE STRAW

This study aimed to evaluate the nutrient composition and in vitro true digestibility of three rice straw (RS) varieties with varying stem lengths (Vasco (V): short-stemmed, Cammeo (C): medium-stemmed, and Efe (E): long-stemmed) treated with urea and/or crude glycerin. Each RS variety was ensiled in laboratory-scale mini-silos for 30 days using the following treatments: i) Control, ii) 5 % urea (U) treated RS, iii) 5 % crude glycerin (G) treated RS, and iv) 5% urea + 5% crude glycerin (UG) treatment RS. The Vasco variety of RS exhibited superior nutritional value compared to the Cameo and Efe varieties, with a greater (P<0.05) in vitro true digestibility of dry matter (IVTDDM (V:46.11%, C:41.17% and E:43.94%) as well as lower (P≤0.001) NDF (V:66.38%, C:71.68%, and E:68.59%) and ADF (V:43.61%, C:50.64%, and E:49.1%) levels. Silage of all cultivars demonstrated comparable responses to the applied treatments. The crude protein (P<0.001) and IVTDDM (P<0.001) were greater in all treatment groups where urea was used alone or in combination with crude glycerin. All cultivars showed a reduction in NDF (P<0.001) and ADF (P<0.001) levels with the application of crude glycerin treatment alone, but no significant effect was observed on IVTDDM values. In the interaction effect, Efe×UG showed higher (P<0.001) in vitro true digestibility while for in vitro NDF digestibility, Efe×U interaction exhibited higher (P<0.001) digestibility compared to other interaction effects. The findings of the study suggest that adding urea or a combination of urea and glycerin to rice straw improved its nutritional content and digestibility. Treating rice straw with a combination of urea (5%) and glycerin (5%) could be a promising strategy to improve its nutritional value for livestock feed. Keywords: crude glycerin, digestibility, rice straw, silage, treatment, urea

Isoacid supplementation influences feed sorting, chewing behaviors, and enteric methane emissions differentially in mid-lactation dairy cows depending on dietary forage level

Past studies have shown that isoacids (ISO) improve dairy cow performance, with effects varying based on dietary forage levels, leading us to speculate that ISO supplementation may also differentially affect enteric methane (CH4) emissions depending on dietary forage levels. Therefore, our primary objective was to examine the effects of ISO supplementation on enteric CH4 emissions in lactating dairy cows fed 2 forage NDF levels (FL), along with monitoring feed particle sorting and chewing behaviors to assess any potential interactions. Sixty-four (64) mid-lactation Holstein cows were utilized in a 10-wk long randomized complete block design trial. Parity, DIM, and prior milk yield (MY) for multiparous cows or genetic merit for primiparous cows were used as blocking factors. Cows were randomly assigned to 1 of 4 diets (n = 16 per diet) with a 2 × 2 factorial arrangement of treatment combinations, including 2 FL, 17 (LF) and 21% forage NDF (HF), without or with ISO supplementation (7.85 mmol/kg DM for isobutyrate and 3.44 mmol/kg DM for 2-methylbutyrate, respectively). Enteric CH4 and chewing activity (rumination and eating time) were measured using the GreenFeed system and sensor-based ear tag system, respectively. The particle size of each diet and ort from individual cows was measured using the Penn State Particle Separator, and a sorting index was calculated. A sorting index of 100 indicates no sorting, while values above or below 100 indicate sorting for or against, respectively. Data were analyzed using a mixed model including FL, ISO, and FL × ISO as fixed effects and block as a random effect (lme4 in R). Our result shows that ISO increased sorting index for long particle in LF (96.1 vs. 109; P < 0.01) but decreased it in HF (100.8 vs. 92.5; P = 0.04). In contrast, ISO did not affect the physically effective particle sorting index (P = 0.51) or intake (P = 0.27) regardless of FL. In alignment with the long particle sorting index, ISO decreased eating and chewing time in the HF but increased them in the LF diet (P < 0.01). In contrast, rumination time was comparable between FL (P = 0.70) and ISO levels (P = 0.19). In the LF diet, ISO supplementation reduced daily CH4 production (g/d) by 9% and intensity (g/kg of MY) by 18% (P < 0.01). In the HF diet, ISO supplementation led to a 10% increase in daily CH4 (P < 0.01) but did not change CH4 intensity (P = 0.17; g/kg of MY) due to improved milk production. Overall, ISO altered feed sorting, feeding behaviors and enteric CH4 emissions depending on FL.

Supplementation of isoacids to lactating dairy cows fed low or high forage diets: Effects on performance, digestibility, and milk fatty acid profile

Our objective was to determine the effects of isoacids (ISO) on the lactation performance, digestibility, and milk fatty acid (FA) profile of Holstein cows fed 2 forage NDF levels (FL). The study was 10-wk long (including 2-wk for covariate) utilizing a randomized complete block design. Sixty-four mid-lactating Holstein cows [662 ± 71 kg BW, 119 ± 51 DIM, 2 ± 0.9 parity] were blocked by parity, DIM, and prior milk yield (MY) for multiparous cows or genetic merit for primiparous cows, and randomly assigned to 1 of the 4 diets (n = 16). Diets were arranged as a 2 × 2 factorial, with 2 FL containing 21 (HF) and 17% forage NDF (LF) without (WIA) or with ISO supplementation (IA, 7.85 mmol/kg DM and 3.44 mmol/kg DM for isobutyrate and 2-methylbutyrate, respectively). Diets were balanced for similar NEL (1.58 Mcal/kg DM), CP (16.0%) and total NDF (27.2%). Feed intake and MY were recorded daily. Nutrient digestibility for each cow was determined using indigestible NDF as a marker, and fecal samples were collected at 8-time points (4 h intervals between samples). Individual cow milk samples composited over a 10-wk period were analyzed using gas chromatography for FA profile. The statistical model included FL, ISO, and FL × ISO as fixed effects and block as a random effect (lme4 in R). The ISO did not affect DMI (P = 0.13), while LF had greater DMI than HF diets (27.8 vs. 26.0 kg/d; P < 0.01). However, ISO increased MY (34.7 vs. 37.2 kg/d; P < 0.01) and ECM (41.9 vs. 39.0 kg/d; P < 0.01) by 7% in HF but not in the LF diet, suggesting FL × ISO interaction (P = 0.04). Interestingly, ISO increased ADG (0.4 kg/d) but decreased MUN by 9% only in LF diet as indicated by FL × ISO interaction (P < 0.01). Additionally, ISO increased DM, OM, NDF, and CP digestibility by 10-24% in HF (P < 0.01), but not in LF (FL × ISO; P > 0.05). As expected, ISO increased milk odd chain FA profiles in the IA groups irrespective of FL, e.g., the IA had greater C15:0 (1.87 vs. 1.54 g/100g FA; P = 0.03) and a tendency to be greater C17:0 levels (0.86 vs. 0.76 g/100g FA; P = 0.05) compared with WIA groups. Overall, ISO improved MY and nutrient digestibility in the HF whereas it increased ADG and decreased MUN in LF diet. Additionally, ISO increased milk odd chain FA (C15:0 and C17:0) regardless of FL.

PSXI-19 Effects of different forage levels on performance and feeding behavior of feedlot cattle

Abstract Body weight (BW) gain is highly correlated with profitability and positively associated with feed efficiency and reduced environmental impact of beef production. Fiber stimulates rumination which is essential for digestion, nutrient absorption, and maintenance of animal health. The quantity and quality of fiber in the diet affect BW gain and feeding and rumination patterns. Forage neutral detergent fiber (fNDF) has a key role in regulating intake and health. This study tested two levels of forage inclusion NDF on performance and feeding behavior of Nellore bulls. Corn silage was the source of forage fiber, diets had 12% or 7% fNDF [23.3% or 13.6% in the total dry matter (DM). Diets had 64% and 73.5% ground corn, and 0.2 and 0.5% potassium chloride respectively for 12% and 7% fNDF. Both treatments included soybean meal (1.5%); cottonseed (7%), urea (1.2%), mineral mixture (2.7%) and monensin (25 ppm). Diets were formulated to meet protein and mineral requirements with RLM model (rlm.app.br). Nellore bulls (n = 40; BW = 460 ± 38 kg and an average age of 20 mo) were fed for 50 d after 21 d adaptation. Each animal was considered as experimental unit in a completely randomized design. On d 21 and 42 behavior was evaluated every 10 min for 24 h. Feed intake was measured using Growsafe feeders. Average daily gain (ADG) was estimated by linear regression based on BW (without fast) obtained in real time scales at the water trough. Data were evaluated by SAS PROC GLM. Daily gain (1.71 vs. 1.43 kg/d, P = 0.0002), DM intake (10.97 vs. 10.00 kg/d, P &amp;lt; 0.001) and feed efficiency (0.154 vs. 0.139, P = 0.04) were greater for the 12% fNDF treatment. Likewise, rumination time was greater for the 12% fNDF compared with the 7% fNDF treatment; 356 and 275 min/d respectively (P = 0.0005). Animals fed 12% fNDF had longer rumination times and improved performance compared with those fed 7%. There were no differences in the number of visits to the troughs or in total feeding time (P &amp;gt; 0.05), with mean visits of 15.17 and 15.43, and feeding times of 145.6 and 146.8 minutes for the 12% and 7% fNDF diets, respectively. Linear correlation between ADG and rumination time was positive r = 0.49 (P &amp;lt; 0.03) and r = 0.28 (P &amp;lt; 0.23), indicating that rumination is associated with an increase in ADG. Coefficient of variation for ADG was 13.4 and 27.2% for 12 and 7% fNDF diets, with greater variability for the low fiber diets. The results highlight the importance of providing adequate forage to feedlot cattle. Results are consistent with previous work from our group demonstrating that the maximum performance is achieved at around 11 to 13% fNDF. Bulls fed 12% fNDF diets had greater BW gain, higher rumination and reduced ADG variation compared with those fed 7% fNDF diets.

141 Determination of the optimum standardized ileal digestible lysine for nursery pigs fed high levels of dietary fiber

Abstract An experiment was conducted to determine the optimum standardized ileal digestible (SID) Lys for nursery swine-fed high levels of dietary fiber. A total of 2,525 pigs [PIC 337 × 1050, initial body weight (BW) = 6.5 kg], placed in pens of 36 mixed-sex pigs, were used in a 42-d trial. Pigs were allotted to 1 of 5 dietary treatments with 14 replicates per treatment. Increasing dietary SID Lys concentrations of 1.14, 1.22, 1.30, 1.38, and 1.45% were obtained by blending the 1.14% and the 1.45% SID Lys diets via Big Dutchman DryExact Pro feeding system (Big Dutchman, Holland, MI). The different SID Lys levels were obtained by changing the inclusion rate of soybean meal, which ranged from 15 to 28%. The experimental diets contained sugar beet pulp and soy hulls, resulting in an NDF level of 10.5%. Experimental diets were fed for 17 d and followed by a common corn, soybean meal, and 10% DDGS diet with 1.30% SID Lys from d 17 to 42. Pigs were weighed on d 0, 17, and 42 to calculate average daily gain (ADG), average daily feed intake (ADFI) and gain to feed ratio (G:F). From d 0 to 17, a quadratic (P &amp;lt; 0.05) improvement in ADG and ADFI was observed for pigs fed increasing SID Lys up to 1.38%, plateauing thereafter. Pigs fed increasing SID Lys had linearly (P = 0.001) improved G:F (Table 1). From d 17 to 42, there was no evidence (P &amp;gt; 0.10) for differences in ADG, yet ADFI increased linearly (P = 0.004) while G:F decreased linearly (P = 0.001) for pigs previously fed increasing concentrations of SID Lys. Overall (d 0 to 42) for pigs fed increasing SID Lys, ADG and ADFI increased quadratically (P &amp;lt; 0.05) up to 1.38% SID Lys, whereas G:F increased linearly (P = 0.001) up to 1.45% SID Lys. Mortality and removal rate was quadratically influenced (P &amp;lt; 0.05) by feeding increasing SID Lys. Our findings suggest feeding weaned pigs from d 0 to 17 a diet containing 1.38% SID Lys is optimal for growth performance while minimizing mortality and removal rate.

Immunomodulatory effect of potassium diformate and sodium diformate on humoral immunity and antioxidant capacity in Acipenser baerii: Cytokine and high-mobility group protein gene induction

The research examined the impact of adding potassium diformate (KDF) and sodium diformate (NDF) to the diet of Siberian sturgeon (Acipenser baerii) and how it influenced their antioxidant levels and immune responses. The fish (n = 315; 17.85 ± 0.2 g initial weight) were fed with seven diets for 56 days, including a control group (unsupplemented diet) and groups with varying levels of KDF (0.15, 0.3, and 0.5%) and NDF (0.15, 0.3, and 0.5%). After the feeding trial, the groups that consumed KDF or NDF demonstrated a dose-responsive elevation in serum superoxide dismutase, catalase, and glutathione peroxidase levels, along with an overall increase in total antioxidant capacity compared to the control group (P < 0.05). These groups also had lower malondialdehyde levels (P < 0.05) and improved immune functions, including higher levels of immunoglobulin M, total protein, albumin, globulin, the activities of lysozyme, respiratory burst, and alternative complement (P < 0.05). Also, the serum bactericidal activity experienced a significant increase (maximum in 0.5% NDF treatment) in the groups subjected to experimental feeding compared to the control group (P < 0.05). The transcription levels of interleukin-1 beta (IL-1β), interleukin 8 (IL-8), tumor necrosis factor-alpha (TNF-α), high mobility group box 1 (AbHMGB1), and high mobility group box 2 (AbHMGB2) genes in the head kidney were significantly upregulated in fish fed with different doses of KDF or NDF compared to the control group (P < 0.05). The most significant upregulation was observed in the group that received 0.5% NDF. Moreover, the experimental groups demonstrated a lower cumulative mortality rate of the fish following exposure to Aeromonas hydrophila AB005 infection compared to the control group (P < 0.05), with the lowest mortality rate observed in the 0.5% NDF or KDF group. Overall, the results suggest that dietary KDF or NDF, especially 0.5% NDF, are promising as environmentally friendly options and sustainable immunostimulants in Siberian sturgeon aquaculture.

Effects of decreasing roughage NDF in diets with high inclusion of DDGS on performance, feeding behavior, and ruminal parameters in Nellore bulls

This study evaluated decreasing levels of roughage NDF (10, 7, and 4 % in DM) in DDGS-based finishing diets. In experiment 1, growth performance, feeding behavior, and carcass characteristics were evaluated on 18 Nellore bulls blocked by initial body weight (BW), with 6 replicates per treatment, in a complete randomized block design. In experiment 2, digestibility, feeding behavior, and ruminal fermentation were evaluated on 6 Zebu crossbred steers in two, 3 × 3 Latin square designs. Differences were considered significant at P < 0.05 and trends were declared at P < 0.10. In experiment 1, DMI increased quadratically with decreasing roughage NDF (rNDF) with the greatest DMI value achieved at 7.43 % rNDF (P<0.01). Average daily gain tended to respond quadratically, with the greatest estimated value occurring at 7.23 % rNDF (P<0.09). Similarly, hot carcass weight and final BW tended to respond quadratically (P<0.09). No effects of roughage NDF level were observed on carcass dressing percentage. Animals that received the lowest dietary rNDF selected for particles longer than 8 mm (P<0.05). Increasing roughage NDF levels in the diet tended to increase time spent ruminating (P<0.06). In experiment 2, a greater inclusion level of rNDF did not affect DMI, but linearly increased NDF intake (P<0.01) and tended to decrease DM digestibility (P<0.09). Ruminal pH remained, on average, above 6. Ruminal ammonia-nitrogen increased up to an inclusion level of 6.6 % rNDF and then decreased (P<0.02). Acetate: propionate ratio linearly decreased with increasing levels of dietary roughage NDF (P<0.02). Decreasing roughage NDF inclusion level in diets with high levels of corn DDGS is viable without compromising performance or increasing the risk of ruminal acidosis occurrence. Diets containing between 7.23 % and 7.43 % of roughage NDF are proposed to be suitable when corn DDGS-based diets are used to maximize intake and weight gain.

Nutrient Value of Pineapple Peel Silage with the Addition of Various Carbohydrate Sources

This study aims to evaluate the nutritional quality of pineapple peel silage with the addition of various carbohydrate sources, namely corn flour, cassava waste, and rice bran. The research used a completely randomized design method with 5 treatments and 4 replications. The treatments are (P0 = 100% pineapple peel; P1 = 70% pineapple peel + 30% corn flour; P2 = 70% pineapple peel + 30% cassava waste; P3 = 70% pineapple peel + 30% rice bran; P4 = 70% pineapple peel + 10% corn flour + 10% rice bran + 10% cassava waste) with the addition of molasses for each treatment as much as 5%. Parameters observed were the nutritional quality of pineapple peel silage including CP%, CF%, DM%, ash (%), and CF%, levels of NDF (%) and ADF (%). The results showed that the difference in carbohydrate source material had a significant effect (P&lt;0.05) on the nutrient content of the pineapple peel silage produced. It can be concluded that the addition of corn flour at a 30% level to pineapple peel silage improves the overall nutritional quality as seen from the increase in crude protein and crude fat as well as a decrease in crude fiber, NDF, and ADF content

Effect of NDF levels of complete pelleted diet and dietary transition period on rumen pH, growth performance, digestibility, and blood indices in fattening male goats

This study aimed to evaluate the effects of low and high NDF levels in complete pelleted diets (CPD) on early diet transition in fattening male goats. Forty male goats were blocked by their initial body weights (BW), and within each block, animals were randomly assigned to one of the four diets using a randomized complete block design. The treatments were arranged in a 2 × 2 factorial comprising of two levels of NDF (20.2% and 32%) and two diet transition strategies (day 7 and 15). All the diets were iso-nitrogenous, having a 15:85 forage-to-concentrate ratio. In the high NDF diet, a higher level of 32% was achieved by replacing corn grain with soy hulls and wheat bran. The goats were housed individually and had ad libitum access to feed and water. During the transition phase, conventional feeding was replaced by the CPD in three steps, and thereafter, all animals had free choice access to their respective diets. Body weight and blood samples were conducted weekly, while rumen samples were collected on day 0, 7 and 15, and then fortnightly. Feeding behavior data was recorded on day 7 during the transition period using video cameras. Nutrient digestibility was performed twice, with n = 4 animals/treatment: 1) after 15 days and 2) at the end of the study. The results revealed that goats fed on a high NDF diet had higher (P < 0.05) DMI and ADG than those fed on a low NDF diet during the transition. Additionally, there was an interaction between NDF and transition days after the transition period and the entire study period. The feed-to-gain ratio was lower (P = 0.05) in goats fed on the LNDF-15 compared with the LNDF-7. The rumen pH of low NDF-7 goats was lower (P < 0.05) throughout the study compared with other treatments. Moreover, the odds for diarrhea were higher (P = 0.03) in the low NDF-7 goats compared to other treatments during and after the diet transition. Male goats fed on a high NDF diet had higher (P < 0.05) nutrient digestibility after transition, but no effect thereafter. Animals fed on a high NDF diet had higher (P < 0.05) DMI, NDF intake, laying, standing time, and rumination time during the diet transition period. Feeding behavior was not recorded after the dietary transition period. In conclusion, feeding HNDF-7 to goats during the diet transition period resulted in improved growth performance, key feeding behavior parameters, and nutrient digestibility. Whereas, during the post-diet transition period, both LNDF-15 and HNDF-7 goats displayed comparable growth performance without any adverse effects on animal health.

Evaluation of multienzyme supplementation and fiber levels on nutrient and energy digestibility of diets fed to gestating sows and growing pigs.

The objective was to investigate the effect of a multienzyme blend (MEblend) and inclusion level on apparent total tract digestibility (ATTD) of energy and nutrients, as well as ileal digestibility of crude protein (CP) and amino acids (AA) in gestation diets with low (LF) or high-dietary fiber (HF) fed to gestation sows. For comparison, growing pigs were fed the same HF diets to directly compare ATTD values with the gestating sows. In experiment 1, 45 gestating sows (parity 0 to 5; 187 ± 28 kg bodyweight; BW) were blocked by parity in a 2 × 3 factorial arrangement and fed 2.2 kg/d of the HF (17.5% neutral detergent fiber; NDF) or LF (13% NDF) diet and one of three levels of MEblend (0.0%, 0.08%, and 0.1%) to determine impacts of MEblend on ATTD. Twenty-seven growing pigs (initial 35.7 ± 3.32 kg BW) were fed the same HF diet (5% of BW) and one of three MEblend inclusions. The MEblend at both 0.08% and 0.1% increased ATTD of energy, NDF, and acid detergent fiber (ADF) (P < 0.05) in gestating sows but ATTD of total non-starch polysaccharides (NSP) and its residues were not affected. Sows fed HF, regardless of MEblend, had greater ATTD of NDF, xylose, and total NSP (P < 0.05) in comparison to grower pigs. In experiment 2, ileal cannulas were placed in 12 gestating sows (parity 0 to 2; BW 159 ± 12 kg) to determine apparent and standardized ileal digestibility (AID and SID) of AA and NSP. In a crossover design, sows were fed the same six diets, as in experiment 1, and a nitrogen-free diet during five periods of seven days each to achieve eight replicates per diet. There was no interaction between diet fiber level and MEblend inclusion. Supplementation of MEblend to gestating sow diets did not impact SID of CP and AA regardless of dietary fiber level. The SID of His, Ile, Lys, Phe, Thr, Trp, and Val were 3% to 6% lower (P < 0.09) in HF than LF independent of MEblend. Supplementation of MEblend did not impact AID of NSP components, but sows fed HF had higher AID of arabinose (LF: 26.5% vs. HF: 40.6%), xylose (LF: 3.5% vs. HF: 40.9%), and total NSP (LF: 25.9% vs. HF: 40.0%) compared to sows fed LF (P < 0.05). Dietary supplementation of MEblend increased ATTD of nutrients, NSP, and energy in diets fed to gestating sows regardless of inclusion level, with MEblend having a greater incremental increase in diets with lower NDF levels. Inclusion of MEblend impacted neither SID of AA nor AID of NSP in low- or high-fiber gestation diets, but high-fiber diet, negatively affected SID of AA.

The role of structural carbohydrates in feeding of cows in the conditions of the european north

The purpose of the research is to study effect of quality of neutral detergent (NDF) and acid detergent (ADF) fiber with its different content in the rations when feeding of high productive cows on the digestibility of nutrients, use of the fodder nitrogen by animals, their productivity. The experiment was carried out on Holsteined cows of the Kholmogory breed during the second phase of lactation (101-200 days) on two groups selected according to the principle of paired analogues - control and experimental, 9 heads each, with a productivity of 8-10 thousand kg per year. The ration of the control group cows corresponded to the economic one. It contained crude fiber - 18.8%, neutral detergent fiber - 42.0%, acid detergent fiber - 21.7% by dry matter. The ration of cows of the experimental group contained crude fiber - 21.7%, neutral detergent fiber - 45.0%, acid detergent fiber - 23.2%. Due to the change in the ratio of bulky feeds (hay, silage) in the ration, different levels of NDF and ADF were created. As a result of the conducted research, it was found that feeding of animal by foods with neutral detergent fiber up to 45.0% and acid detergent fiber up to 23.2%, microbiological activity in the rumen of cow is increasing, the digestibility of nutrients in the ration is increasing, nitrogen metabolism is improving. As a result milk productivity is increasing by 7.0%, milk fat by 0.08%. Maintenance of high productive cows on ration with the level of NDF - 45.0% and ADF - 23.2% makes it possible for more fully stabilization of metabolic processes in animal body, to improve the intensity of rumen digestion, and use feed efficiently. Acknowledgment: This work was carried out within the framework of the state assignment of the Ministry of Science and Higher Education of the Russian Federation on the topic No. FGER-2021-0001, registration number AAAA-A19-119011790116-7. We would like to express our gratitude to the staff of LLC "Polar Star", Murmansk Region, for their assistance in conducting the research.

PSX-A-1 Impacts of Undigestible NDF and Grain Source in Feedlot Steers on Performance, Feeding Behavior, and Ruminal pH

Abstract Little is known about how fiber quality impacts rumen health in feedlot diets with minimal forage inclusion. The objective of the study is to investigate the interaction between undigestible NDF (uNDF) levels and grain fermentability (GF) on animal performance, feeding behavior, and ruminal pH in feedlot steers. Using a 2x2 factorial design, Angus crossbred steers (n=96, 505±54 kg) were fed an isonitrogenous and isocaloric diet containing: high uNDF (haylage; 12% uNDF) or lower uNDF (corn silage, 6% uNDF) and high (high moisture corn) or lower ruminally fermentable grain (rolled corn) yielding 4 different treatments. Following adaption, steers were fed their treatment ration for 102±14d before slaughter. Feed intake (DMI) and feeding behavior data were collected using Insentec feeders and continuous ruminal pH was measured during the last 4 weeks before slaughter using indwelling pH probes (12 steers/treatment). Liver abscesses and gut health scores were assessed at slaughter. Steers on the low uNDF diet had greater ADG (P≥0.005) and improved F:G (P≥0.005) for the first half of the finisher period. No other differences in performance traits were found for the second half or overall finisher period between uNDF and GF, or their interaction. DMI (P≥0.64) did not differ, but time spent at the feeder (min/day) was greater (P≥0.02) for low GF steers. Steers fed low uNDF had greater (P≥0.04) time per day spent with ruminal pH below pH 5.8. At slaughter, steers fed high GF treatments had greater hot carcass weights (P≥0.03). This data suggests that low uNDF can improve early gain but may result in increased SARA risk compared to high uNDF at the same forage inclusion level. However, there was limited interaction with grain processing. Further research should continue to investigate relationships between uNDF and ruminal pH, and dietary uNDF concentrations that optimize performance without compromising gut health.

Response of Phytogenic Additives on Enteric Methane Emissions and Animal Performance of Nellore Bulls Raised in Grassland

The objective of this study was to evaluate the intake and digestibility of nutrients, emission of enteric CH4, and productive performance of Nellore bulls grazing Urochloa brizantha cv. Marandu palisade grass pastures during the rainy season, receiving an energy supplement or mineral supplement, with or without the inclusion of phytogenic additives. Forty-eight Nellore bulls were treated with: (1) energy supplement without the inclusion of phytogenic additives; (2) energy supplement with the inclusion of phytogenic additives; (3) mineral supplement without the inclusion of phytogenic additives; and (4) mineral supplement with the inclusion of phytogenic additives. Consumption of total dry matter (DM), crude protein (CP), apNDF, and energy; digestibility of DM, CP, and energy; average daily gain; stocking rate; and gain per area were higher in animals consuming energy supplements than those consuming mineral supplements. Digestibility of DM, NDF, and energy levels were lower in animals that consumed phytogenic additives. Compared with mineral supplements, the supply of energy supplements provides higher nutrient intake, increases enteric CH4 emission, and improves nutrient digestibility, providing a greater productive performance. The inclusion of phytogenic additives negatively affected nutrient intake and digestibility, did not reduce enteric CH4 emission, and influenced productive performance.

The Effect of Different Lactic Acid Bacteria Inoculants on Silage Quality, Phenolic Acid Profiles, Bacterial Community and In Vitro Rumen Fermentation Characteristic of Whole Corn Silage

Corn silage is an important source of forage, but whether or not bacterial inoculants should be applied is somewhat controversial in ruminant feeding practice. In the present study, chopped whole corn plants treated with a single inoculant of Lactobacillus buchneri (LB), Lactobacillus plantarum (LP), Pediococcus pentosaceus (PP) served as either homofermentation (e.g., lactate only) or heterofermentation (e.g., lactate and acetate) controls and compared with those treated with either a mixture of the lactic acid bacteria (QA: 60% LP, 10%PP, 30% LB) or a mixture of the lactic acid bacteria (QB: 60% LP, 15% PP, 25% LB), to investigate their effects on the fermentation quality, ester-linked phenolic acids, and in vitro digestibility. After 60 day ensiling, the addition of QA exhibited the lowest pH (3.51) with greater lactic acid (LA) production. The ester-linked ferulic acid (FAest) and p-coumaric acid (pCAest) concentrations were significantly decreased during 60 days ensiling. And among all these groups, the LB and QA treated group showed a lower concentration of FAest and pCAest than other groups. After 60 days ensiling, Lactobacillus was the dominant genus in all LAB treated groups. Meanwhile, negative correlations of Bacillus, Bacteroides, Bifidobacterium, Blautia, Prevotella, Ruminococcus, and Roseburia with FAest content after 60 days ensiling occurred in the present study. Komagataeibacter was mainly found in LB and PP addition silages, and presented a significant negative effect with the level of acid detergent fiber (ADF). To explore whether the addition of LABs can improve digestibility of whole corn silage, an in vitro rumen fermentation was conducted using the 60 day ensiled whole corn silages as substrates. The QA addition group exhibited a greater 48 h and 96 h in vitro dry matter and ADF disappearance, greater 48 h gas production and less methane emissions. Even though there were the same neutral NDF levels in corn silages treated with LB and QA after 60 days ensiling, the QA treated silages with lower FAest and pCAest presented higher IVDMD after 96 h and 48 h in vitro fermentation. In brief, the addition of mixed inoculants of 60% LB,10% PP, 30% LB compared with the addition of whichever single HoLAB or HeLAB inoculants, facilitated the release of ester-linked phenolic acids (e.g., ferulic and p-coumaric acids) and remarkably, improved silage quality in terms of sharp pH decline and greater lactate production. Taken together with the improvement in rumen microbial fermentation, the results obtained in the present study provided concrete evidence for the role of mixed LAB application in corn silage preparation for ruminant feeding practices.

Nitrogen fertilization strategies with protected urea for tropical grasses in an integrated crop-livestock system

The intercropping of corn and grass allows for the production of grains, and simultaneously, the recuperation and renovation of pastures. This study aimed to evaluate the effects of N doses on the yield, structure, chemical-bromatological characteristics, and the N use efficiency of Brachiara brizantha cultivars in an integrated crop-livestock system. The experimental design was completely randomized, with four replications, in a slip-plot scheme. The plots were formed of two grass plants, Marandu and Paiaguás, intercropped with corn and subplots composed of nitrogen doses (70, 100, and 130 kg N ha-1). During the system’s establishment (1st cycle), the Paiaguás grass presented higher values for all morphogenic and structural characteristics and higher forage yield when compared to Marandu grass. After the corn harvest, the interaction effect of the N doses and the cultivars for the foliar structure emergence and the number of living leaves was observed. The variables foliar elongation rate, stem elongation rate, and phyllochron were not influenced by any of the investigated factors (P&gt;0.05). The highest DM content was obtained with the application of 100 kg ha-1 of N, whereas, for the other chemical characteristics, there was only an effect among the cultivars. Paiaguás grass exhibited the highest levels of NDF, ADF, lignin, and MM. CP levels were not influenced by the factors studied. Marandu grass and the use of 70 kg N ha-1 are recommended for intercropping with corn

Optimal level of physically effective neutral detergent fibre in corn stover cracked-corn-based finishing diets on the growth performance, dietary energetics, carcase characteristics, and nutrient digestion in fattening lambs

Two trials were conducted to determine the optimal physically effective neutral detergent fibre (peNDF) level in corn stover-cracked-corn-based finishing diets for feedlot lambs. Concentrations of peNDF (10.0, 12.5, 15.0, and 17.5%) were controlled by replacing cracked corn grain with corn stover. In Trial 1, 32 Rambouillet × Katahdin intact male lambs (body weight [BW], 28.47 ± 2.70 kg) were used in a 63-d feeding trial. Dry matter intake (DMI), average daily gain (ADG), and feed efficiency (GF) were greater at the 12.5% peNDF level (quadratic component, P <.05). The dressing percentage increased linearly (p <.01) as peNDF decreased, but hot carcase weight (HCW) was greater for lambs fed 12.5% peNDF (quadratic effect, p <.01) than those fed different concentrations. In Trial 2, eight ruminally cannulated Pelibuey lambs (29.3 ± 4.2 kg BW) were used in a replicated 4 × 4 Latin square design to evaluate the effects of the treatment on total tract digestion. Ruminal pH, registered at 4-h post-feeding, linearly decreased (p <.01) from 6.70 to 5.77 as the level of peNDF decreased. The digestibility of dry matter (DM), organic matter (OM), and NDF increased linearly (p <.01) as the peNDF level decreased, but the apparent digestible energy (Mcal/kg) showed a quadratic effect (p <.01) with a peNDF of 12.5%. The findings show that the estimated optimal peNDF level in cracked-corn-based finishing diets for lambs in the finishing phase of fattening is approximately 11.5%. HIGHLIGHTS Physically effective NDF levels in finishing rations for lambs were tested; the study provides better understanding of peNDF level on finishing lambs Levels lower than 12.5% and greater than 15.0% peNDF decrease performance and carcase yield Total tract digestion and energy efficiency were maximal at 11.5% peNDF

The Effect of Physically Effective Neutral Detergent Fiber on Milk Composition and Milk Yield

This study examines the effect of the physically effective Neutral Detergent Fiber (peNDF) value of roughage on milk composition and milk yield. The physically effective NDF varies depending on the particle length of the roughage and the amount of structural and non-structural carbohydrates in the diet. The quality, quantity, and particle length of the roughage are crucial for a healthy rumen. The longer the particle length of the roughage is, the more the cow ruminates and the more saliva it produces. The produced saliva is used to balance the rumen pH. It is aimed to protect rumen and animal health, improve milk and fertility, and make more efficient animal husbandry through the optimum preparation of physically effective NDF levels in the ration. The optimum NDF in the ration realizes the balance of energy, protein, and fiber required by cows in the early lactation period and makes it possible to maximize the microbial yield and protect the rumen's health. In this period, the lactation peak is maintained by promoting dry matter intake. The current study showed that the effect of peNDF on milk composition properties and somatic cell number significantly differed between farms (P

Deep transcriptomic study reveals the role of cell wall biosynthesis and organization networks in the developing shell of peanut pod

BackgroundPeanut (Arachis hypogaea L.) belongs to an exceptional group of legume plants, wherein the flowers are produced aerially, but the pods develop under the ground. In such a unique environment, the pod’s outer shell plays a vital role as a barrier against mechanical damage and soilborne pathogens. Recent studies have reported the uniqueness and importance of gene expression patterns that accompany peanut pods’ biogenesis. These studies focused on biogenesis and pod development during the early stages, but the late developmental stages and disease resistance aspects still have gaps. To extend this information, we analyzed the transcriptome generated from four pod developmental stages of two genotypes, Hanoch (Virginia-type) and IGC53 (Peruvian-type), which differs significantly in their pod shell characteristics and pathogen resistance.ResultsThe transcriptome study revealed a significant reprogramming of the number and nature of differentially expressed (DE) genes during shell development. Generally, the numbers of DE genes were higher in IGC53 than in Hanoch, and the R5-R6 transition was the most dynamic in terms of transcriptomic changes. Genes related to cell wall biosynthesis, modification and transcription factors (TFs) dominated these changes therefore, we focused on their differential, temporal and spatial expression patterns. Analysis of the cellulose synthase superfamily identified specific Cellulose synthase (CesAs) and Cellulose synthase-like (Csl) genes and their coordinated interplay with other cell wall-related genes during the peanut shell development was demonstrated. TFs were also identified as being involved in the shell development process, and their pattern of expression differed in the two peanut genotypes. The shell component analysis showed that overall crude fiber, cellulose, lignin, hemicelluloses and dry matter increased with shell development, whereas K, N, protein, and ash content decreased. Genotype IGC53 contained a higher level of crude fiber, cellulose, NDF, ADF, K, ash, and dry matter percentage, while Hanoch had higher protein and nitrogen content.ConclusionsThe comparative transcriptome analysis identified differentially expressed genes, enriched processes, and molecular processes like cell wall biosynthesis/modifications, carbohydrate metabolic process, signaling, transcription factors, transport, stress, and lignin biosynthesis during the peanut shell development between two contrasting genotypes. TFs and other genes like chitinases were also enriched in peanut shells known for pathogen resistance against soilborne major pathogens causing pod wart disease and pod damages. This study will shed new light on the biological processes involved with underground pod development in an important legume crop.

Palm oil cake in milk production and quality of dairy cows: Systematic review, meta-analysis and principal component analysis

Studies have shown beneficial effects of palm oil on the diet of lactating cows, with an increase or maintenance of the nutritional value of milk. However, the high levels of NDF and lignin and the lower concentration of non-fibrous carbohydrates in palm oil cake have shown negative effects on ruminal digestibility and food intake, therefore, on the amount of energy available for milk production. This study aimed to carry out a systematic review, meta-analysis, and principal components analysis to assess the effects of palm oil cake on the milk quality parameters and production by dairy cows. The search resulted in 8 references and 21 eligible studies. The distribution of data for independent variables was explored with BoxPlot. The meta-analysis was used to summarize the grouped effect of palm oil cake on milk production and quality parameters, using the fixed-effect model or random-effects model. The sampling variability assumed in the random-effects model was estimated by Cochran's Q test and the I2 statistic by Higgins and Thompson. The weighted mean difference (WMD) and standardized mean difference (SMD) summarized the size of the palm oil effect on the response variables. The factors involved in the variability expressed in the meta-analysis were investigated with the principal components analysis. The publication bias of the studies was assessed using the test by Begg and Mazumdar (1994) and Egger et al. (1997). The heterogeneity between the studies was nonexistent or very low (I2 < 3). The symmetry of the funnel graph pointed to no publication bias in the studies (P> 0,05). In comparison to the control diet, cows fed with palm oil cake showed an increase (0,19%, P <0,005) in the concentration of milk fat. In conclusion, the inclusion of 10% to 32% of palm oil cake in diets of dairy cows with an average production of 12,8 (kg day−1) of milk does not affect milk production and the yield (lactose, protein, and fat) and concentration (lactose, protein) of the milk components; and increases the concentration of milk fat by up to 0,19%.

Ruminal and Fecal Bacteriome of Dairy Calves Fed Different Levels and Sources of NDF.

Simple SummaryThe present study aimed to characterize and understand how the gastrointestinal microbiome (in rumen and feces) of pre-weaning dairy calves is affected by feeding a starter concentrate with two levels of fiber and the inclusion of hay in the diet. Another study objective was to verify if the effects on the microbiome remained after weaning. The ruminal bacteriome was not affected by levels or sources of fiber. The fecal bacteriome was affected by age and diet. The inclusion of hay affected the fecal microbial diversity.A starter concentrate containing different levels and sources of NDF can modify the gastrointestinal bacteriome. This study evaluated 18 Holstein calves housed in un-bedded suspended individual cages, fed one of three treatments: 22NDF: a conventional starter containing 22% NDF (n = 7); 31NDF: a starter with 31% NDF, replacing part of the corn by soybean hull (n = 6); and 22Hay: diet 22NDF plus coast-cross hay ad libitum (n = 5). All animals received 4 L of milk replacer daily, weaned at 8th week of age, and housed in wood shelters until week 10. To evaluate the bacteriome, the bacterial community of ruminal fluid and fecal samples was determined by sequencing V3 and V4 region amplicons of the 16S rRNA gene. Bacterial diversity in rumen was not affected by diet or age. The phyla Firmicutes and Bacteroidota, and Prevotella’ genus were the most abundant in ruminal fluid and fecal samples. In feces, the α-diversity indices were higher for 22Hay. All indices were significantly affected by age. We believe that the ruminal bacteriome was affected by basal diet components, but not affected by NDF levels or sources. The supply of hay was effective in modifying the fecal bacteriome of dairy calves due to hind gut fermentation.