The field experiment was conducted during rabi season, 2023-24 at research farm, Vivekananda Global University, Jaipur. The experiment was layout in Randomized Block Design (RBD). The treatments consisted of biofertilizers like Azotobacter and Phosphate Solubilizing Bacteria (PSB) and three level of Molybdenum (400, 600 and 800 ppm) and control. The soil in the experimental area was sandy loam with pH (7.81), Organic Carbon (0.21%), Available N (132.5 kg/ha), Available P (21.13 kg/ha) and Available K (203.24 kg/ha). Seed rate is 100 kg/ha. The application of biofertilizers + PSB + 800 ppm Mo significantly increased the Plant height (110.46 cm), Dry matter accumulation (542.54 g/m2), No. of tillers/ running row meter (93.51), Number of effective tillers m-2 (199.86), Number of grains spike-1 (53.60), Grain yield (3450 kg/ha) and Straw Yield (6562 kg/ha) of barley over the control.