Hemoglobin Concentration Levels Research Articles

Antianemic Activity of Allolobophora caliginosa Extract against Phenylhydrazine-induced Anemia in Rats

Background:: Anemia is a global hematological disorder. Earthworms have antimicrobial, antispasmodic, antihypertensive, antiallergic, anti-inflammatory, and anticoagulant properties. Objective:: Our study aimed to investigate the anti-anemic activity of the earthworm Allolobophora caliginosa extract (AcE) on phenylhydrazine-induced hemolytic anemia in rats. Methods:: Eighteen adult male albino rats were randomly divided into 3 groups (n = 6 per group) as follows: Control, PHZ, and AcE-treated groups. Induction of anemia was done by intraperitoneal administration of phenylhydrazine (40 mg/kg) for 2 consecutive days. Treatment of AcE by intraperitoneal injection (45 mg/kg) for six successive days. Results:: Treatment of anemic rats with AcE extract caused significant increases in red blood cells (RBCs), hemoglobin (HB), hematocrit (HCT), total protein (TP), albumin, glutathione reduced (GSH), catalase (CAT), and glutathione-S-transferase (GST), as compared to the PHZ group. While, mean corpuscular haemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), malondialdehyde (MDA), and nitric oxide (NO) levels significantly decreased after intraperitoneal administration of AcE, when compared to the PHZ group. Liver sections from the AcEtreated group restored the normal micromorphological features of the liver. The expression of caspase- 3 was weak and/or there was no expression in the liver sections from AcE groups. AcE caused a significant reduction in the incidence of DNA damage, which appeared as less DNA in the tails. Conclusion:: The present research sheds light on the antianemic efficacy of AcE in rats. This study reveals that AcE has antianemic activity, which can be linked to its iron and branched-chain amino acid content, as well as antioxidant and anti-apoptotic properties.

Comparison between Rapid Test and ELISA in Cytomegalovirus Detection among Pregnant Women in Kirkuk City

Introduction: Cytomegalovirus is member of herpes virus that has a phospholipid-rich membrane, capsids, and a network of DNA. As this virus affects many living cell in the body, people are at risk of acquiring it especially immuno-compromised patients like pregnant women and can transmit it to their fetus therefore the diagnosis of the virus is important for monitoring the infection in mother and prevent transition to the fetus. Aims: The purpose of this study is to compare two common methods for Cytomegalovirus diagnosis. Materials and method: CMV Rapid Test was performed to detect IgG & IgM Abs specific for CMV using CTK BIOTECH/USA kit. Second method was ELISA technique that performed for further validation using Bioactive/Germany kit. CBC test also performed to compare (WBC, Hb. Lymphocyte, platelet) count between patients and control groups. Results: By rapid test, all patients tested positive for IgG, however only (10.16%) of 128 patients tested positive for CMV IgM, according to ELISA, which shows that the number of IgM positivity was only (8.59%). According to complete blood count we indicated that there were significant differences between patients and control regarding to WBC count and lymphocyte count with P value (0.0001) and (0.0001) respectively. No significant differences observed in hemoglobin concentration and platelet level between two groups. Conclusions: Viral infections require accurate diagnosis to minimize negative outcomes and the results showed that Cytomegalovirus diagnosis in pregnant women via ELISA test is more accurate, specific and sensitive than rapid test.

Insights Into Colorectal Cancer Screening: A Multidatabase Cohort Study of Over 1.5 Million Taiwanese

IntroductionColorectal cancer (CRC) remains a significant public health concern. This study aims to provide a comprehensive understanding of the effectiveness of fecal immunochemical test (FIT) screening on CRC incidence and mortality, leveraging the scale of over 1.5 million randomly selected Taiwanese participants and more than 11.7 million person-years of follow-up. MethodsThis prospective cohort study merges data from three robust Taiwanese health databases: the CRC screening program, cancer registration, and death registration databases. Incidence and mortality rates of CRC were calculated based on age, sex, urbanization, and past screening status. Cox proportional hazard models were used to assess the association between screening statuses and CRC incidence or mortality, adjusting for age, sex, and urbanization levels. Statistical analysis of the data was conducted in 2021-2022. ResultsFIT screening was associated with a 33% reduction in CRC incidence and a 47% reduction in mortality. The study identified a dose-response relationship between the fecal hemoglobin concentration (f-HbC) levels and CRC risk. Participants with consistent FIT-negative results had significantly reduced CRC incidence and mortality risks, while those with one or more positive FIT results faced increased risks. Notably, compliance with follow-up examinations after a positive FIT significantly lowered mortality risk. ConclusionsThis large-scale study validates the efficacy of FIT screening in reducing CRC incidence and mortality. It offers a nuanced understanding of how various screening statuses impact CRC risks, thus providing valuable insights for public health strategies aimed at CRC prevention.

Hydroxyurea dose optimisation for children with sickle cell anaemia in sub-Saharan Africa (REACH): extended follow-up of a multicentre, open-label, phase 1/2 trial

Realizing Effectiveness Across Continents with Hydroxyurea (REACH) is an open-label non-randomised trial of hydroxyurea (hydroxycarbamide) in children with sickle cell anaemia in sub-Saharan Africa. The short-term results of REACH on safety, feasibility, and effectiveness of hydroxyurea were published previously. In this paper we report results from extended hydroxyurea treatment in the REACH cohort up to 8 years. In this open-label, non-randomised, phase 1/2 trial, participants were recruited from four clinical sites in Kilifi, Kenya; Mbale, Uganda; Luanda, Angola; and Kinshasa, Democratic Republic of Congo. Eligible children were 1-10 years old with documented haemoglobin SS or haemoglobin Sβ zero thalassaemia, weighing at least 10 kg. Participants received fixed-dose hydroxyurea of 17.5 (±2.5) mg/kg per day for 6 months (fixed-dose phase), followed by 6 months of dose escalation (2·5-5·0 mg/kg increments every 8 weeks) as tolerated, up to 20-35 mg/kg per day (maximum tolerated dose; MTD), defined as mild myelosuppression. After the MTD was reached, hydroxyurea dosing was optimised for each participant on the basis of changes in bodyweight and laboratory values over time (MTD with optimisation phase). After completion of the first 12 months, children with an acceptable toxicity profile and favourable responses were given the opportunity to continue hydroxyurea until the age of 18 years. The safety and feasibility results after 3 years has been reported previously. Here, haematological responses, clinical events, and toxicity rates were compared across the dosing phases (fixed-dose hydroxyurea vs MTD with optimisation phase) as protocol-specified outcomes. REACH is registered on ClinicalTrials.gov (NCT01966731) and is ongoing. We enrolled 635 children between July 4, 2014, and Nov 11, 2016. 606 children were given hydroxyurea and 522 (86%; 266 [51%] boys and 256 [49%] girls) received treatment for a median of 93 months (IQR 84-97) with 4340 patient-years of treatment. The current (Oct 5, 2023) mean dose is 28·2 (SD 5·2) mg/kg per day with an increased mean haemoglobin concentration (7·3 [SD 1·1] g/dL at baseline to 8·5 [1·5] g/dL) and mean fetal haemoglobin level (10·9% [SD 6·8] to 23·3% [9·5]) and decreased absolute neutrophil count (6·8 [3·0] × 109 cells per L to 3·6 [2·2] × 109 cells per L). Incidence rate ratios (IRR) comparing MTD with fixed-dose hydroxyurea indicate decreased vaso-occlusive episodes (0·60; 95% CI 0·52-0·70; p<0·0001), acute chest syndrome events (0·21; 0·13-0·33; p<0·0001), recurrent stroke events (0·27; 0·07-1·06; p=0·061), malaria infections (0·58; 0·46-0·72; p<0·0001), non-malarial infections (0·52; 0·46-0·58; p<0·0001), serious adverse events (0·42; 0·27-0·67; p<0·0001), and death (0·70; 0·25-1·97; p=0·50). Dose-limiting toxicity rates were similar between the fixed-dose (24·1 per 100 patient-years) and MTD phases (23·2 per 100 patient-years; 0·97; 0·70-1·35; p=0·86). Grade 3 and 4 adverse events were infrequent (18·5 per 100 patient-years) and included malaria infection, non-malarial infections, vaso-occlusive pain, and acute chest syndrome. Serious adverse events were uncommon (3·6 per 100 patient-years) and included malaria infections, parvovirus-associated anaemia, sepsis, and stroke, with no treatment-related deaths. Hydroxyurea dose escalation to MTD with dose optimisation significantly improved clinical responses and treatment outcomes, without increasing toxicities in children with sickle cell anaemia in sub-Saharan Africa. US National Heart, Lung, and Blood Institute and Cincinnati Children's Research Foundation.

Prevalence and predictors of anemia among six-week-old infants in Kwale County, Kenya: A cross-sectional study.

Anemia is a significant public health problem among children worldwide. The etiology of anemia is multifactorial but iron deficiency (ID) is the most common cause of anemia in low- and middle-income countries. ID and anemia in infancy can impair growth and cognitive development. The aim of this study was to determine the prevalence and predictors of anemia among six-week-old infants in Kwale County, Kenya. This cross-sectional study included 424 mother-infant pairs. Structured questionnaires were administered to the mothers to obtain information on socio-demographic variables, maternal characteristics and birth information. Anthropometric data was collected for each child. A heel prick was done to measure hemoglobin and zinc protoporphyrin concentration levels. Chi-square test, bivariate and multivariate regression analyses were done to determine factors associated with anemia. The prevalence of ID, anemia and IDA was 60.4% (95%CI: 55.9-65.2), 21.0% (95%CI: 17.5-25.2) and 15.8% (95%CI: 12.7-19.7) respectively. Bivariate analysis showed that the risk of anemia was significantly higher among male infants (odds ratio (OR) = 2.20 (95%CI: 1.33-3.63), p = 0.002), iron deficient infants (OR = 2.35 (95%CI: 1.39-3.99), p = 0.001) and infants from Msambweni Sub-County (OR = 2.80 (95%CI: 1.40-4.62), p<0.001). Multivariate analysis revealed that odds of anemia were significantly higher in infants born to mothers who did not use iron supplements during pregnancy (adjusted odds ratio (aOR) = 74.01 (95%CI: 2.45-2238.21), p = 0.013 and significantly lower in infants born to mothers with parity ≥ 4 (aOR = 0.05 ((95%CI: 0.00-0.77), p = 0.024). In six-week-old infants in rural Kenya, anemia prevalence was 21.0% with ID accounting for 75.3% of anemia cases. Given the physical and cognitive impairments associated with ID and anemia in early infancy, it may be prudent to re-evaluate the current Kenyan pediatric protocols to include anemia screening and potential treatment of infants less than 6-months of age.

Understanding Anemia among Women of Reproductive Age and Children in Namibia: A Comprehensive Review

Anemia is one of the leading causes of death globally, not only among children and women; however, there have been records of deaths among men too. Anemia disproportionately affects young children and expecting mothers with increased morbidity and mortality. Who estimates that anemia affects 30% of women aged 15 to 49, 37% of pregnant women, and 40% of children aged 6 to 59 months globally. The goal of lowering anemia among women of reproductive age has not been met, and 25.2% of women between the ages of 15 and 49 are now affected in Namibia. Namibia with a current prevalence rate of 46.1% of children under 5 years, is a severe global public health issue. According to the morphological classification of anemia, normocytic normochromic anemia (86.3%) was the most common kind in Namibia, with a moderate frequency of iron deficiency anemia whereas the highest prevalence of vitamin b12 deficient anemia was found in some regions. Anemia indicates both poor nutrition and poor health and is often categorized based on its cause. Iron deficiency, pregnancy, antiretroviral therapy side effects, and hereditary blood abnormalities increase the risk of anemia. Depending on the hemoglobin levels in the blood, anemia can be either short-term or long-term. For pregnant women and children under the age of 5, mild anemia is defined as a level of hemoglobin concentration between 10.0 and 10.9g/dl, moderate anemia is defined as a level between 7.0 and 9.9g/dl, and severe anemia is defined as a level below 7.0g/dl. Medical professionals follow different standards such as typical treatment recommendations, World Health Organization recommendations, and reference materials while dealing with individuals who have suspected anemia.

Результаты выращивания молоди тиляпии на продукционных комбикормах с добавлением антиоксиданта астаксантина

One of the main stages of metabolism in the body is the digestibility and digestibility of feed nutrients, the ef-fectiveness of which to a certain extent depends on the use of biologically active substances in diets that have antioxidant properties and have a stimulating effect on vital body functions. An important criterion for choosing feed additives is their environmental safety. An ideal antioxidant should be easily absorbed by the body and prevent the formation of free radicals at physiologically significant levels. Therefore, the use of natural antioxidants, namely carotenoids, is of particular interest. Carotenoids play a different role in the metabolism of fish and are also pronounced antioxidants that protect the body from the action of free radicals. The experience of using the natural antioxidant astaxanthin in feeding tilapia juveniles is considered. Astaxanthin is a powerful antioxidant that has a huge impact on the functioning of all systems and overall health. But the practice of its use has not affected mass thermophilic aquaculture species, such as tilapia, whose muscle tissues and caviar are not stained under the action of astaxanthin, and its effect on the physiological status of fish, as well as antioxidant properties have not been studied. It was found that the addition of astaxanthin to the composition of production feeds at a dosage of 20.0; 30.0; 40.0 mg/kg allowed to increase productivity by 38.5; 30.1; 17.03%, respectively, and also had a positive effect on the physiological state of the fish. As a result, after feeding, the indicators of total protein in the experimental variants 1-3 were higher than the control group by 48.3; 41.6 and 31.6%, respectively. As a result of the analysis of the data obtained after the completion of experimental work, a significant increase in the level of hemoglobin concentration in experimental variants 1-3 was established by 1.2-1.3 times, respectively, compared with the control variant.

Protective role of olive oil extract of propolis on short and long-term administration of tamoxifen in rats.

Tamoxifen (TAM) is an antiestrogenic agent used for adjuvant treatment in estrogen receptor-positive breast cancers in the pre/post-menopausal period. This study, it was aimed to determine the effect of olive oil extract of propolis (OEP) on short and long-term administration of TAM in rats. Wistar albino rats were divided into groups with eight animals in each. Groups: control, OEP, TAM, and OEP + TAM. At the end of the experiment, oxidative stress tests were performed with Enzyme-Linked ImmunoSorbent Assay (ELISA)on blood and tissue samples (liver, kidney, and ovary) taken from rats. After single-dose TAM administration, there was a significant increase in red blood cell, hematocrit, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration levels compared to the control group, a decrease in low-density lipoprotein (LDL) value, a significant increase in liver enzymes and fasting glucose values was detected compared with the control and propolis groups. A normalizing effect was observed in the group given OEP and TAM combined. The increase in Malondialdehyde (MDA) and the decrease in enzyme activities in tissues are also noteworthy. Propolis application reduced the tissue damage caused by TAM. In addition, improved cytokine levels, which increased with TAM administration. It has been concluded that OEP can be given in supportive treatment, as it improves hematological and antioxidant parameters in TAM treatment.

Influence of the antioxidant astaxanthin in the composition of feeds on the physiological state of heat-water objects of aquaculture

This scientific study examines the experience of using astaxanthin in tilapia feeding, and its effect on the physiological state of individuals. The work used a set of methods used in fisheries research — variation statistics and physiological and biochemical tests. The reliability and validity of the scientific provisions and conclusions contained in this work are determined by a significant amount of factual material; in total, 150 tilapia individuals were studied. The data obtained during the study indicate that the use of astaxanthin at a concentration of 20.0; 30.0; 40.0 and 50.0 mg gives the best physiological and biochemical parameters of cultivated individuals, in comparison with the control variant. However, taking into account the maximum input of astaxanthin in feed for warm-water fish species, the most effective and safe concentration of astaxanthin in the diet of warm-water aquaculture objects is 40.0 mg/kg of feed, which is also confirmed by the results of the physiological state of individuals obtained during the experiments. One of the main stages of metabolism in the body is the digestibility and assimilation of feed nutrients, the effectiveness of which to a certain extent depends on the use of biologically active substances in diets that have antioxidant properties and have a stimulating effect on the vital functions of the body. An important criterion for the choice of feed additives is environmental safety. As a result, after feeding, the indicators of total protein in the experimental variants (1, 2, 3 and 4) were higher by 27.8; 58.5; 59.5 and 52.2%, respectively, of the control group. Analyzing the data obtained after the completion of the experimental work, a significant increase in the level of hemoglobin concentration in the experimental variants was found to be 1.2–1.3 times, respectively, compared with the control variant.

Altered granulocyte count and erythrocyte measures in middle-aged, healthy carriers of APOE and PICALM risk genes for Alzheimer's disease.

APOE‑ε4 genotype (apolipoprotein E, epsilon 4) is the strongest genetic risk factor for Alzheimer's disease (AD). Despite years of research, it is still not known how it contributes to dementia development. APOE has been implicated in many AD pathology mechanisms, like Aβ clearance, brain metabolism, changes within microglia and other glial functions and inflammatory processes. In fact, immunological/inflammatory processes are recently discussed as an important factor in Alzheimer's development and granulocyte profiles changes are reported in patients. However, the exact link between the immune system and risk‑genes is unknown. In particular, it is not known whether and how they interact throughout the lifetime, before the disease onset. The aim of the study was to investigate the relationship between granulocyte count and the APOE/PICALM genes in healthy individuals with an increased genetic risk of AD. An exploratory analysis regarding other blood cells was also conducted. Blood samples were collected from 77 healthy middle‑aged (50-63 years old) participants, who were also asked to complete a health and life‑style questionnaires. Groups with different AD risk‑genes were compared. Differences in granulocyte profiles were found in healthy carriers of AD risk‑genes who had slightly elevated eosinophil levels as compared to non-risk carriers. An exploratory analysis showed some alteration in mean corpuscular hemoglobin content and concentration (MCH/MCHC) levels between risk‑carriers subgroups and non-risk carriers. No other differences in blood count or lipoprotein profile were found between healthy APOE/PICALM risk‑carriers and non-risk carriers. Longitudinal studies will reveal if and how those changes contribute to the development of AD pathology.

Fulminant Type 1 Diabetes-East and West.

Fulminant type 1 diabetes is a subtype of type 1 diabetes in which beta cells are destroyed within days or a few weeks. The first criterion indicates a rise in blood glucose levels shown in the patient's history. The second suggests that the increase occurs suddenly within a very short period, as shown by the laboratory findings of the discrepancy between the glycated hemoglobin concentration and plasma glucose level. The third indicates a marked reduction in endogenous insulin secretion, which indicates almost complete destruction of beta cells. Fulminant type 1 diabetes is a common subtype in East Asian countries, including Japan, but rare in Western countries. Class II human leukocyte antigen and other genetic factors may have contributed to the skewed distribution. Environmental factors may also be involved including entero and herpes viruses and immune regulation during drug-induced hypersensitivity syndrome; pregnancy may also affect it. In contrast, treatment with an immune checkpoint inhibitor of the anti-programmed cell death 1 antibody induces similar characteristics and incidence of diabetes as fulminant type 1 diabetes. Further studies are needed to clarify the etiology and clinical characteristics of fulminant type 1 diabetes. Although the incidence of this disease differs between the East and West, it is life-threatening; thus, it is important to diagnose fulminant type 1 diabetes without delay and treat it appropriately.

Previously Undiagnosed Anemia and Iron Deficiency Anemia among Preschool Children at Taif City

Introduction: In the Arab Gulf countries, anemia, including iron deficiency anemia )IDA(, considered one of the main public health problems. Although, the World Health Organization reported that IDA as the most common type of anemia, which made up half of all anemic cases all over the world, there were limited research performed among preschool children about anemia in Saudi Arabia. Thus, the aim of this study was to investigate the prevalence of previously undiagnosed anemia and IDA among children between 2 to 6 years old. In this study, several risk factors that might contribute to the high prevalence of anemia were assessed. Methods: The study was carried out in Taif city during the period from January 2023 to April 2023. Blood samples were collected from 200 preschool children and analyzed for hematological parameters as well as related biochemistry tests to investigate the prevalence of previously undiagnosed anemia and IDA. Results: The majority of the study participants were Saudi 99% and aged 4 to 6 years 72.5%. Most of children participants, 92.5% had normal haemoglobin (Hb) concentration and normal levels of red blood cells (RBCs) and normal hematocrit indicating their non-anemia health status. In this study, previously undiagnosed anemia were reported within 6% of children indicated by low Hb concentration, low RBC count and abnormal low hematocrit level. Out of these undiagnosed anemic children, there were 25% diagnosed with IDA. Conclusions: Since, anemia and IDA are still a public health problem among preschool children in Saudi Arabia this study suggested that the hematological blood examination should be done to students as a requirement before school which may reveal the presence of previously undiagnosed anemia and might associated with low mantle and functional performance among children. Moreover, further studies are recommended at all regions of the Kingdome to investigate the underlying related risk factors, which will provide more insight ....

The Relationship Between Nutritional Anemia and Acne: A Case-Control Study.

Background In the past decade, there has been growing interest in identifying the relationship between nutritional status and acne. Many dietary factors have been studied, including milk, fast food, and chocolate. However, nutritional anemia,which is a common problem inyoung individuals, has not been well investigated. Objectives The objective of this study was to determine the relationships between acne and nutritional anemia among people in the Qassim Regionof Saudi Arabia. Methods This study used a case-control design. It targeted people aged from 15 to 25 years old in the Qassim Region of Saudi Arabia. This study was conducted using a database of Electronic Health Records (EHR) data from the Qassim University outpatient department (OPD). Data analysis was conducted by using SPSS (IBM Inc., Armonk, New York). Results A total of 114 of the study populationwere involved in this study. The acne group represented was identical to the control group. The mean age of study participants was 23.1 ± 4.19 years old, and the majority of them were females (86%). Moreover, the patient group had relatively lower levels of mean corpuscular volume (MCV), vitamin B12, ferritin, mean corpuscular hemoglobin (MCH), and hemoglobin than the control group without any significant correlation, whereas the patient group had higher levels of mean corpuscular hemoglobinconcentration (MCHC) and red cell distribution width (RDW) without significant difference. Our results showed that the prevalence of anemia among respondents was 17.5%, and a higher prevalence of anemia was observed in the control group without significant differences. Furthermore, the patient group had a significantly higher prevalence of vitamin B12 deficiency (38.6%) than the control group (p=0.041). Conclusion Our results concluded that patients with acne vulgaris in the Qassim Region of Saudi Arabia had asignificantly higher rate of vitamin B12 deficiency. Additional studies are needed to confirm this association.

Evaluation of isolated probiotics on the efficacy of immune system in male and female Wistar rats

Probiotics were isolated from fruits and vegetables. Microscopic, biochemical, and molecular tests were carried out for the characterization of strains of probiotics. To assess the effects of isolated probiotics on immunity, male and female (15 + 15) Wistar rats (n = 3) were randomly distributed into 5 groups: 0-day, negative control, positive control (commercially available Lactobacillus acidophilus-14), laboratory isolated probiotics with accession numbers; Lactobacillus plantarum (MZ707748) and Lactobacillus plantarum (MZ729681), respectively. After hematological investigations, the amounts of IgA and IgG in male and female groups were significantly different (p < 0.05). At the same time, the values of Alanine-transaminase (ALT) and Aspartate-aminotransferase (AST) in both genders were average, and there were no differences (p > 0.05). Male probiotic-treated groups had decreased levels of interleukin-6, bilirubin, and creatinine, but female probiotic-treated groups had a slight rise in bilirubin and creatinine values (p = 0.05). Cellular blood count levels of Hematocrit (HCT) and white blood cells (WBC) in male groups showed considerable differences (p < 0.05), while there were no differences (p > 0.05) in female groups. Levels of Red blood cells (RBC) and mean corpuscular hemoglobin concentration (MCHC) showed distinct changes (p < 0.05) in female groups, while these values were insignificant changes (p > 0.05) among male groups. There were considerable differences between the control and groups that were given probiotics. Histopathological results showed no damage to the liver and thymus. A fecal examination of rats was used to examine the viability and survival of Lactobacilli. Based on blood tests, it was observed that the immune system was boosted and improved in probiotic-treated groups compared to control groups.

Determination of the Efficacy of Broccoli Sprouts Seeds on Growth, Blood Parameters, and Immunological Indicators in Experimentally Immunosuppressed Male Rats

This study was conducted in graduate laboratories, College of Agriculture, University of Baghdad’s and animal house in College of Veterinary Medicine, University of Tikrit to determine the effectiveness of broccoli sprouted seeds on growth parameters, blood components and measurement of immune indicators in male rats with experimentally reduced immunity. The results of the study showed that oral administration of Sandimmun to male albino rats led to a decrease in the weight gain rate of laboratory rats and a decrease in blood components such as the white blood cell count (WBC), red blood cell count (RBC) and hemoglobin concentration level (Hb), while there was a significant increase in the number of platelets (PLT). It also caused a significant decrease in the values of the immunological indicators like IgG, IgA, and IgM. The results showed that feeding on broccoli sprouted seed powder with both concentrations 15% and 30% caused an increase in the weight gain rates to 53.40 and 84.40 g, respectively, compared to the average weights gained for the rats with reduced immunity in treatment G2, which was 15.40 g. The same applies to the effect of feeding on the two treatments G3 and G4. These groups had significant improvement in the blood parameters of rats in both treatments, but the G4 treatment was the best treatment in raising the level of white blood cell counts WBC, which amounted to 14.90x109 cell/ml while the number of RBC erythrocytes was at 8.18x106 cell/ml compared with their numbers in the experimentally immunocompromised group of rats, which were at 15.12x109 cell/ml and 8.06 x106 cells/ml, respectively. While the concentration of hemoglobin (Hb) level was at 13.68 mg/dL, the number of Platelets (PLT) 485x103 platelets / microliter and all these values were significantly similar with their numbers in the values of treatment G1. Also, feeding at a concentrations of 15% and 30% led to increase the immunoglobulins IgG, IgA and IgM in rats, as it caused an increase in their values in treatment G3, which were at 0.48, 0.44, 0.52 mg/dL, respectively. While their values in treatment G4 were at 0.54, 0.47, and 0.56 mg/dL, compared with their values in treatment G2, which amounted to 0.35, 0.33, 0.41 mg/dL. The conclusion of this study is that feeding with broccoli sprouted seed powder improved the growth rates, blood profile parameters and immune globulins in the experimentally reduced immune male rats.

Evaluation of Electrochemotherapy with Bleomycin in the Treatment of Colorectal Hepatic Metastases in a Rat Model

The available ablative procedures for the treatment of hepatic cancer have contraindications due to the heat-sink effect and the risk of thermal injuries. Electrochemotherapy (ECT) as a nonthermal approach may be utilized for the treatment of tumors adjacent to high-risk regions. We evaluated the effectiveness of ECT in a rat model. WAG/Rij rats were randomized to four groups and underwent ECT, reversible electroporation (rEP), or intravenous injection of bleomycin (BLM) eight days after subcapsular hepatic tumor implantation. The fourth group served as Sham. Tumor volume and oxygenation were measured before and five days after the treatment using ultrasound and photoacoustic imaging; thereafter, liver and tumor tissue were additionally analysed by histology and immunohistochemistry. The ECT group showed a stronger reduction in tumor oxygenation compared to the rEP and BLM groups; moreover, ECT-treated tumors exhibited the lowest levels of hemoglobin concentration compared to the other groups. Histological analyses further revealed a significantly increased tumor necrosis of >85% and a reduced tumor vascularization in the ECT group compared to the rEP, BLM, and Sham groups. ECT is an effective approach for the treatment of hepatic tumors with necrosis rates >85% five days following treatment.

Biochemical and Hematological Findings of Halabja Victims: 34 Years after Sulfur Mustard Exposure.

The city of Halabja situated in Kurdistan-Iraq faced a deadly attack using chemical warfare including the use of sulfur mustard (SM) in 1988. The survivors of this attack also faced the consequences of exposure to the toxic chemical SM in the form of multiple health complaints. The aim of this study is focused to gather data about the biochemical and hematological findings of Halabja victims who suffered from Sulfur Mustard (SM) exposure after completing 34 years of the attacks. A total of Twenty-five non-smoker patients (f: m19:6, mean age 59.2 years (range 35-85) and ten non-smokers control, healthy people (f: m 3:7, mean age 25.3 years (range 20-37) were interviewed and subjected to be tested. A purposive sampling strategy was adopted to recruit the study participants in August 2022. There were no significant differences between patients and controls regarding thyroid function markers. The levels of total protein and total albumin were significantly lower in the victims than in the controls (total protein: 7.67 ± 0.55 g/dL, P < 0.05, albumin: 4.30 ± 0.26 g/dL, P < 0.01). In addition, the serum high-density lipoprotein (HDL) was decreased significantly in patients compared to control groups (43.02 ± 8.15 mg/dL, P < 0.01). Moreover, triglyceride, low-density lipoprotein (LDL) and total cholesterol were not considerably increased in the patients. On the other hand, hematological parameters did not show a significant difference except for the mean corpuscular hemoglobin concentration (MCHC) level significantly lower in the victims than in the controls (33.48 ± 0.56 g/dL, P < 0.01). Finally, there were significant differences in total iron and ferritin levels in the groups. From this study, it was concluded that some of the victim's biochemical factors can be influenced owing to the long-term consequences of SM. From the similarity of functional test results of thyroid and hematology between groups, it is also stated that biochemical changes detected may be due to a patient's delayed respiratory complications.

Iron Deficiency and Anemia in Male and Female Adolescent Athletes Who Engage in Ball Games.

The aim of this study was to assess the prevalence of iron deficiency (ID) and iron deficiency anemia (IDA) among male adolescent athletes who participate in non-calorie-restricting sports, and to compare the results with female athletes of the same age and sports. Data of the hemoglobin concentration (Hb) and serum ferritin (sFer) levels of male (n = 350) and female (n = 126) basketball and football players, aged 11-18, from two sport medicine centers in Israel were gathered and analyzed. Mild ID was defined as sFer ≤ 30 µg/L, moderate as sFer ≤ 20 µg/L, and severe as sFer ≤ 10 µg/L. IDA was defined as sFer ≤ 20 µg/L and Hb < 13 g/dL for males and sFer ≤ 20 µg/L and Hb < 12 g/dL for females. The prevalence of mild ID was 41.1% and 53.2%, moderate was 17.4% and 27.8%, and severe was 2% and 4.8% in males and females, respectively. The prevalence of IDA was 2.6% in males and 4% in females. Mild and moderate ID was significantly higher among females. In conclusion, non-anemic ID, which is known to be common among female athletes, especially in sports requiring leanness, is also highly prevalent among adolescent males playing ball games. Therefore, screening for hemoglobin and sFer is recommended for young athletes of both genders and in all sports.

The study of oxygenation dynamics during experimental tumor growth using frequency-domain diffuse optical spectroscopy

The objective of this work was to study the dynamics and mechanisms of oxygenation changes of an experimental rat tumor Plyss lymphosarcoma in the process of its growth using diffuse optical spectroscopy (DOS). DOS in trans-illumination configuration utilizing high-frequency (140 MHz) modulation of light intensity is used to assess absorption and scattering coefficients averaged over tumor tissue at several wavelengths 684, 794, and 850 nm. Those values were used to assess concentrations of tissue oxyhemoglobin and deoxyhemoglobin and oxygen saturation. During the observation period (from the 5th to the 15th day after transplantation) the tumor volume increased up to 25 times. In the process of tumor growth, a gradual increase in the content of deoxyhemoglobin, a decrease of oxyhemoglobin level, and a decrease of blood oxygen saturation without changes in the level of total tissue hemoglobin concentration were observed. Thus, the main mechanisms of oxygen saturation decrease in the process of tumor growth are associated with two effects: the increase of oxygen consumption rate (demonstrated by the increase of deoxyhemoglobin) and the decrease of oxygen supply (demonstrated by the decrease of oxyhemoglobin).

Prevalence of malaria and associated clinical manifestations and myeloperoxidase amongst populations living in different altitudes of Mezam division, North West Region, Cameroon

BackgroundMalaria is a growing problem in Africa, with prevalence varies from areas to areas due to several factors including the altitude. This study aimed to investigate the malaria distribution and its relationship with level of some blood parameters and plasma myeloperoxidase (MPO) in population of three localities with different altitudes.MethodsA total of 150 participants were recruited in each locality and facial body temperature of each was measured using a forehead digital thermometer. Blood samples were collected and used diagnose malaria parasite using the rapid test followed by Giemsa stain microscopy and have the full blood count and MPO level using a colorimetric method.ResultsThe overall prevalence of falciparum malaria was 34.7%, with no difference between the three communities, but Bambili of high altitude had the highest prevalence (70.7%). A majority of the infected persons had mild malaria, with most cases being asymptomatic (temperature < 37.5 ºC). Patients had significant increase of geometric mean malaria parasite density (GMPD) in Bambili (1755 ± 216 parasites/µL) and Bamenda (1060 ± 2515 parasites/µL of blood) than patients in Santa (737 ± 799 parasites/µL). There was a significant risk to have malaria infection in Bambili (OR = 33.367, p = 0.021) than in Santa (OR = 2.309, p = 0.362). Bambili’ participants of 6–10 years showed a high prevalence of malaria (85.7%). GMPD was significantly different between males (p = 0.010) as well as females (p = 0.000). Participants from Santa (11.2 ± 3.2 g/dL) and Bambili (12.6 ± 2.4 g/dL) had a high haemoglobin concentration than those from Bamenda (10.6 ± 2.1 g/dL). There was a significant difference in the WBC counts and platelet counts among infected participants in the study areas. MPO level had an increasing trend among infected participants in Santa (2.378 ± 0.250), Bambili (2.582 ± 0.482) and Bamenda (2.635 ± 0.466).ConclusionThe results of the present study demonstrated that altitudinal variations significant impact the risk of population to have malaria with high parasitaemia and may contribute to the malaria prevalence and severity by affecting the haemoglobin concentration, WBC and platelet level and plasma MPO in population.