Tomatoes (Lycopersicon esculentum L.) are the most extensively grown vegetable in the world. It belongs to the Solanaceae family and is commonly planted for its tasty fruits. Pests, weeds, diseases, and parasites are just a few of the numerous variables that significantly affect tomato growth and yield. The most common disease affecting tomatoes is fusarium wilt. Fifteen rhizobacterial strains were identified by morphological and biochemical analyses in this work, and they were employed as an antagonist against Fusarium oxysporum f.sp. lycopersici. Fifteen isolates were investigated for their antagonistic properties against Fusarium oxysporum f.sp. lycopersici using an invitro dual culture approach. The growth of Fusarium oxysporum f.sp. lycopersici was suppressed by each isolate. Out of the 15 rhizobacteria isolates, isolate RBS-5 exhibited the highest level of growth inhibition and strongly suppressed the development of Fusarium oxysporum f.sp. lycopersici, resulting in a 57.28 percent reduction in pathogen growth as compared to the control. The development of Fusarium oxysporum f.sp. lycopersici was suppressed by isolates RBS-12, RBS-6, and RBS-15, in decreasing order of merit, compared to the control by 53.7, 51.91, and 51.73 percent. Isolate RBS-13 showed the least amount of pathogen growth inhibition 20.83 percent. The data was statistically analyzed.