Group Level Research Articles

Vitamin D has therapeutic effects on obesity and hyperandrogenemia in PCOS mouse model induced by low dose DHEA and high-fat diet

Polycystic ovary syndrome (PCOS) is the most complex and common reproductive endocrine disease among reproductive age women. This study aimed to investigate the effects of vitamin D (Vit.D) in a PCOS mouse model induced by low dose DHEA and high-fat diet. Prepubertal female mice were divided into 4 groups randomly: control, PCOS, PCOS with low dose Vit.D(LDVD), and PCOS with high dose Vit.D(HDVD) groups (n = 10 per group). PCOS mice were administrated with high-fat diet and subcutaneous injection with 6 mg/kg/day dehydroepiandrosterone throughout the study. After the first 30 days, 1,25(OH)2D3 was intend to be administered by intraperitoneal injection for 40 consecutive days, 1.3 µg/kg/week in LDVD group, and 13 µg/kg /week in HDVD group. However, the mice in HDVD group appeared to be fatigue and anorexic after the Vit.D injections, then all died within two weeks. The body weights and testosterone levels in PCOS group were significantly higher than those in the control and LDVD groups (P < 0.001). The total cholesterol levels in the control group were lower than those in PCOS and LDVD groups (P < 0.001). Further, the ratio of liver to body weight was different among groups (P < 0.001). Our data illustrates that Vit.D has therapeutic effects on obesity and hyperandrogenemia in PCOS mouse model induced by low dose DHEA and high-fat diet. However, over dose of Vit.D is toxic. Further researches are needed to elucidate the mechanisms.

Effects of anger and trigger identity on triggered displaced aggression among college students: based on the “kicking the barking dog effect”

BackgroundDrawing on the “kicking the barking dog effect”, this study investigated the individual and group-level mechanisms underlying triggered displaced aggression (TDA).MethodsThree experiments were conducted to investigate the effects of anger and hostile attribution on TDA, examining these factors at both the individual and group levels. The preliminary experiment investigated how emotions affect subsequent cognition at an individual level with the aim of understanding the underlying generative process of TDA. Experiment 1 explored the relationship between anger, hostile attribution, and subsequent aggressive behaviors at an individual level, while also discussing the underlying generative process of TDA. Experiment 2 investigated the relationship between anger, hostile attribution, and subsequent aggressive behaviors from a group-level perspective, while also delving into the impact of trigger identity on the underlying generative process of TDA.ResultsWhen individuals were exposed to both a provocation and a subsequent triggering situation, they demonstrated stronger hostile attribution and displayed more aggressive behavior directed toward the trigger. This study found that hostile attribution played a complete mediating role in the influence of anger on TDA, while the triggering situation itself had a moderating role. In the presence of triggering situations, individuals exhibited stronger hostile attribution as their level of anger increased. In the absence of a triggering situation, changes in anger level did not have a significant effect. In the presence of a triggering situation, the identity of the trigger played a moderating role in the process of “anger → hostile attribution → TDA”. When the trigger belonged to an in-group, individuals exhibited stronger hostile attribution toward the out-group and subsequently displayed greater TDA.ConclusionThese findings expand the applicability of the “kicking the barking dog effect” and offer suggestions for controlling the escalation of intergroup conflicts.

Elevated plasma progranulin levels in the acute phase are correlated with recovery of left ventricular function in the chronic phase in patients with acute myocardial infarction

Background Progranulin is a secreted glycoprotein that regulates inflammation and wound healing. However, plasma progranulin levels in the acute phase and their clinical significance in patients with acute myocardial infarction (AMI) remain to be elucidated. Objective We aimed to investigate the relationship between the increase in plasma progranulin levels in the acute phase and the recovery of left ventricular function in the chronic phase in AMI patients. Method and result Eighteen AMI patients were followed up for 6 months. Blood samples were collected from the antecubital vein on day 0 (on admission) and day 7 in the acute phase. The control group consisted of patients without significant coronary artery stenosis, as assessed by cardiac catheterization (n = 16). Plasma progranulin levels were measured by enzyme-linked immunosorbent assay. Echocardiography was performed in the acute (within 7 days) and chronic (6 months) phases of AMI to evaluate left ventricular ejection fraction using the modified Simpson’s method. Plasma progranulin levels in the AMI group on day 0 (69.5 ± 24.6 ng/mL) were similar to those in the control group (84.2 ± 47.1 ng/mL). There was a significant increase in progranulin levels in the AMI group on day 7 (104.2 ± 52.0 ng/mL) compared with day 0. The increase in plasma progranulin levels in the acute phase was positively correlated with the increase in left ventricular ejection fraction between the acute and chronic phases. Among various factors, only plasma progranulin levels were favorably correlated with left ventricular functional recovery in the chronic phase. Conclusion The increase in plasma progranulin levels in the acute phase may serve as a predictive biomarker and a contributer for the recovery of left ventricular function in the chronic phase in patients with AMI.

Constructing representative group networks from tractography: lessons from a dynamical approach

Human group connectome analysis relies on combining individual connectome data to construct a single representative network which can be used to describe brain organisation and identify differences between subject groups. Existing methods adopt different strategies to select the network structural features to be retained or optimised at group level. In the absence of ground truth, however, it is unclear which structural features are the most suitable and how to evaluate the consequences on the group network of applying any given strategy. In this investigation, we consider the impact of defining a connectome as representative if it can recapitulate not just the structure of the individual networks in the cohort tested but also their dynamical behaviour, which we measured using a model of coupled oscillators. We applied the widely used approach of consensus thresholding to a dataset of individual structural connectomes from a healthy adult cohort to construct group networks for a range of thresholds and then identified the most dynamically representative group connectome as that having the least deviation from the individual connectomes given a dynamical measure of the system. We found that our dynamically representative network recaptured aspects of structure for which it did not specifically optimise, with no significant difference to other group connectomes constructed via methods which did optimise for those metrics. Additionally, these other group connectomes were either as dynamically representative as our chosen network or less so. While we suggest that dynamics should be at least one of the criteria for representativeness, given that the brain has evolved under the pressure of carrying out specific functions, our results suggest that the question persists as to which of these criteria are valid and testable.

Molecular mechanisms of ferroptosis in renal ischemia-reperfusion injury Investigated via bioinformatics analysis and animal experiments.

Kidney transplantation is a pivotal treatment for end-stage renal disease. However, renal ischemia-reperfusion injury (IRI) during surgery significantly impacts graft function. Despite unclear molecular mechanisms, no specific therapies or preventative measures are available. Gene expression profiles from renal biopsies before and after IRI were downloaded from public databases. Differentially expressed genes were identified using the Wilcoxon rank-sum test and weighted gene co-expression network analysis. Ferroptosis-associated genes were screened using the FerrDb database. The genes with the highest connectivity were identified via the protein-protein interaction (PPI) network and upstream regulatory miRNAs were found through the gene-miRNA network. A mouse renal IRI model was constructed for transcriptome sequencing and quantitative real-time polymerase chain reaction (qRT-PCR) validation to elucidate the relationship between key ferroptosis genes and regulatory miRNAs in renal IRI. Differential analysis identified 15 ferroptosis-associated genes (TNFAIP3, IL6, KLF2, EGR1, JUN, ZFP36, GDF15, CDKN1A, HSPB1, BRD2, PDK4, DUSP1, SLC2A3, DDIT3, and CXCL2) involved in renal IRI regulation. In animal experiments, ferroptosis-related genes were also upregulated in the model group. Enrichment analysis and hematoxylin-eosin pathological staining suggested these genes are primarily involved in renal inflammatory responses. PPI network analysis revealed IL6 as the gene with the highest connectivity, and the gene-miRNA network indicated IL6 might be regulated by miR-let-7a. Animal experiments revealed decreased miR-let-7a and increased IL6 levels in the model group, identifying potential therapeutic targets. MiR-let-7a regulates ferroptosis in renal IRI by targeting IL6, highlighting IL6 as a crucial gene in the ferroptosis process of renal IRI.

Hypernatremia is associated with mortality in severe elderly sepsis patients

Objective To explore the relationship between hypernatremia and 28-day mortality in elderly sepsis patients. Methods A total of 179 elderly patients (age ≥65 years) with elevated serum sodium admitted to the Department of Critical Care Medicine of Nanjing Hospital affiliated with Nanjing Medical University from September 2021 to September 2022 were included in this retrospective observational study. The clinical data of all patients were collected, and the patients were divided into septic group and nonseptic groups according to the Sepsis 3.0 definition. The clinical features, acute physiological and chronic health II score (APACHE II score), mechanical ventilation time, serum sodium value and duration of serum sodium elevation were compared between the two groups. ROC curves were drawn to evaluate the predictive value of each index on the prognosis of sepsis patients, and Kaplan‒Meier survival analysis was carried out on patients with different serum sodium peaks. Results (1) The changes in serum sodium within 48 hours after admission in the sepsis group were small and statistically significant compared with those in the nonsepsis group (P = 0.039); however, the serum sodium elevation duration was longer (P = 0.018). (2) Compared with nonseptic patients, the 7-day mortality of septic patients was higher (15.8 vs. 7.7, P&lt;0.001). The 28-day mortality of septic patients was higher than that of nonseptic patients, but there was no significant difference between the two groups (P = 0.086). (3) The serum sodium level in the sepsis group was higher than that in the nonsepsis group on the 1st, 3rd, 5th and 7th days (P&lt;0.001). There was no significant difference in mechanical ventilation time or duration of stay in the ICU between the two groups. (4) The ROC curve analysis showed that the peak value of serum sodium had predictive value for the prognosis severity of elderly patients with sepsis. The area under the curve (AUC) was 0.753, the 95% confidence interval (95% CI) was 0.639~0.867, and the best cut-off value was 154.9 mmol/L. (5) According to the best cut-off value of the serum sodium peak, the septic patients were divided into two groups: the peak value of serum sodium was ≥154.9 mmol/L (group A), and the peak value of serum sodium was &lt;154.9 mmol/L (group B). Among them, the case fatality rate was higher at 7 days and 28 days when the peak value of serum sodium was ≥154.9 mmol/L (group A) (22.0% vs. 8.6%); the χ2 value was 35.379, P&lt;0.05; 75.6% vs. 37.1%, χ2 = 14.21, P = 0.003). There was no significant difference in mechanical ventilation time or duration of stay in the ICU between the two groups. (6) Kaplan‒Meier survival analysis showed that the median survival time of patients with a serum sodium peak ≥154.9 mmol/L (group A) was significantly shorter than that of patients with a serum sodium peak &lt; 154.9 mmol/L (group B) (16.7±1.4 d vs. 24.8±1.2 d, P &lt;0.05). Conclusions The serum sodium increase in elderly sepsis patients lasts for a long time, and the serum sodium fluctuation is relatively small. The serum sodium peak value has predictive value for 28-day mortality.

Thyroid hormones and prognosis in adults with status epilepticus: a retrospective study

ObjectivesThyroid hormone levels have been indicated to be associated with the functional outcome in critical illness. However, the studies on thyroid hormones and status epilepticus (SE) are rare. This study aimed to evaluate the predictive value of serum thyroid hormone levels on admission for unfavorable outcome in adult patients with SE.MethodsWe investigated and validated the predictive value of serum thyroid hormone levels on admission for the prognosis of adult SE patients. We extracted the clinical information and outcomes of patients. Modified Rankin scale (mRS) scores were applied to assess the patients’ functional outcome, and mortality at 30 days after SE onset was identified. Serum levels of thyroid hormones including free thyroxin (FT4), free triiodothyronine (FT3) and thyroid-stimulating hormone (TSH) were detected on admission.ResultsWe first analyzed the discovery cohort of 87 patients with SE. We found that 35.6% (31/87) of the patients had a poor outcome at discharge, and 18.4% (16/87) of the patients died during hospital stay and at 30-day follow up. The serum FT3 levels in the non-survivors group were significantly lower than those in the survivors group. Low T3 syndrome occurred in 29.9% (26/87) of SE cases and patients with low T3 syndrome were more likely to have unfavorable outcomes. Furthermore, we observed similar results in the external cohort, which validated our findings.ConclusionsSerum FT3 levels measured on admission are independently associated with 30-day mortality in SE patients. Additionally, low T3 syndrome may be a promising candidate for predicting SE prognosis.

Prevalence and patterns of pre-competition weight loss practices in Chinese Amateur boxers

This study investigated the weight loss (WL) practices of Chinese amateur boxers using the Rapid Weight Loss Questionnaire (RWLQ). A total of 701 (563 males, 138 females) boxers participated in the study and were categorized by sex, age group, and competitive level. Sixty-seven percent of boxers purposefully engaged in WL practices before competition. The average habitual WL was 6.0% (5.8% for juniors and 6.3% for seniors) of body mass (BM), with the average highest WL was 9.5% (9.1% for juniors and 10.1% for seniors) of BM. Most participants (69% for juniors and 84% for seniors) allocated 15+ days for WL before competition. No significant differences in habitual WL%, highest WL%, and rapid weight loss score (RWLS) were found between age groups or competitive levels (all p &gt; 0.05). However, males’ highest WL% and RWLS were significantly higher than females (p &lt; 0.001, p = 0.002, respectively). International boxers began WL later than local boxers (15.5 vs. 14.3 years, p = 0.012). National boxers began WL later than provincial and local boxers (15.6 vs. 14.8 years, p &lt; 0.001; 15.6 vs. 14.3 years, p &lt; 0.001). Increased exercise and training in plastic suits were the most frequently used WL methods. Coaches were identified as the most influential person concerning boxer's WL practices, surpassing doctors or nutritionists. This study found that some WL practices among Chinese boxers differ from those in other sports and countries. Although the prevalence of WL among junior boxers was relatively low, the magnitude of WL was high, warranting more attention in both academic and practical fields.

Factors influencing management of dry cell battery waste: a case of Greater Accra Region in Ghana

Indiscriminate disposal of dry cell battery (DCB) waste contributes to environmental and public health issues in developing countries such as Ghana, due to the toxic nature of this specific waste. Accordingly, a study was conducted in Accra, Ghana, to determine the socio-economic and demographic factors influencing handling DCB waste, aiming a sustainable environment. Using a random sampling technique, a descriptive cross-sectional survey was conducted, encompassing 367 respondents from the Accra-Tema Metropolitan areas and Tema West Municipal Assembly in Greater Accra, Ghana. Using descriptive and multivariate statistical methods, the survey data were analysed with the Statistical Package for Social Sciences (SPSS) version 27. The results of this study show that female gender and residential area are likely to positively influence the use of DCB at home. Education significantly affects the use of DCB and its proper disposal. The results also suggest that 78% of the respondents disposed of DCB waste in waste bins. The mean monthly income of the respondents stands at USD 270, which is average and likely partially to positively influence the disposal of the DCB. The data collected revealed that female gender, age group, family size, and education level influence the indiscriminate disposal of DCB waste and DCB waste recycling. The results highlight that educated females above the age of 55, with a monthly income, are likely to properly segregate DCB waste. This study contributes to the knowledge gap in relation to dry cell battery waste management (DCBWM) in developing countries, aiming to advance global sustainability. This study is expected to contribute to educate and create awareness in managing DCB waste to reduce its indiscriminate disposal which leads to environmental pollution and negatively affects human health and environmental sustainability in Ghana.

Absolute versus Relative Poverty and Wealth: Cooperation in the Presence of Between-Group Inequality

Abstract While inequality in resource endowments has been shown to affect cooperation levels in groups, much of this evidence comes from studies of within-group inequality. In an online public goods experiment, we instead examine the effects of payoff-irrelevant inequality in resources between groups on cooperation within equal groups, i.e. the effect of mere exposure to inequality. When all groups are poor or rich, their contribution behaviour is very similar. Relative inequality, when poor and rich groups coexist, leads to lower contributions in rich groups. We find suggestive evidence that this may be related to stereotypes about the rich contributing less than the poor.

Bioelectrical stimulation therapy for muscle injuries in aerobics athletes

This article aims to explore the recovery effect of the bioelectrical stimulation therapy on muscle injuries in aerobics athletes. Non-invasive medical techniques are adopted to activate muscle tissue through electrical currents, promoting muscle contraction ability and functional recovery. This article selects 100 aerobics athletes with muscle injuries through questionnaire surveys and interviews. The Modulo Plus electrical stimulation device is used, and personalized treatment plans are set. Muscle changes before and after treatment are monitored using electromyography and ultrasound technology. In the single-blind experiment, in the fourth week, the pain score of the experimental group decreases to 2.4 points; the functional recovery score increases to 75.2 points; the flexibility measurement reaches 19.2 cm. In the case-control study, the bioelectrical stimulation therapy cures all athletes in the sixth week, exceeding the conventional therapy’s 35 patients, and has a lower recurrence rate. In the cohort study, athletes who use the bioelectrical stimulation therapy for a long time have a shorter average recovery time of 15.3 days and a recurrence rate of 16%. In the muscle recovery experiment, in the eighth week after treatment, the electromyographic activity level of the experimental group increases to 58.6 μV; the muscle thickness increases to 4.3 mm; the echo intensity increases to 63.1 dB; the fatigue characteristic score drops to 2.1. These data indicate that the bioelectrical stimulation therapy has significant effects in reducing pain, promoting functional recovery, improving flexibility, shortening recovery time, and reducing recurrence rates and pain scores, thereby providing an effective treatment option for the recovery of muscle injuries in aerobics athletes.

Effects of a social network enhancement intervention for older adults: a feasibility study

BackgroundSocial networks play a critical role in the mental health of older adults. This pilot study investigates the feasibility of a newly developed intervention to enhance older adults’ social networks. This intervention was designed on the Theory of Mind’s foundation and aimed to enhance older adults’ social interaction motivation through theoretical explanations. Furthermore, the courses fostered more social opportunities for the participants through group-based sessions.MethodsThe feasibility of this intervention was tested using a double-blind, two-arm, non-randomized grouping approach. Older individuals residing in two separate residential buildings (n = 31, mean age = 66.81, 48% women) were divided into an intervention group (n = 15) and a control group (n = 16). They attended daily group sessions at a designated location and completed homework assignments. The primary outcomes of this pilot study were the feasibility of the intervention, and secondary outcomes included Theory of Mind levels and social network indicators. Additional outcomes encompassed levels of global mental health and depression.ResultsAll participants completed the pilot intervention and completed assessments. The primary outcomes indicated that the intervention had excellent feasibility, including compliance (attendance and homework completion rates met the standards) and satisfaction (average ratings for all items ranged from 4.47 to 5.00 on a 5-point scale). Interview results revealed that participants in the intervention group found the intervention beneficial for their daily lives and expressed a desire to participate in a formal intervention. Regarding secondary and additional outcomes, compared to the control group, the intervention group exhibited a significant improvement in emotional recognition performance of Theory of Mind. There was a significant increase in the whole network density in the intervention group. There were no significant differences in other social network indicators, global mental health, and depression levels in the intervention group compared to the control group.ConclusionsThe social network enhancement intervention for older adults is feasible. This pilot study has identified several improvements in the courses and tests. It is necessary to carry out a formal course to examine the effectiveness of the intervention on social networks in older adults.Trial registrationRegistration number: ChiCTR2100053779; Reg Date: 29/11/2021.

Pentoxifylline improves anemia through its novel effect on hypoxia-inducible factor-2 alpha in hemodialysis patients: a randomized, double-blind, placebo-controlled clinical trial

ABSTRACT Objectives This randomized, double-blind, placebo-controlled clinical trial aimed to prospectively examine the effect of pentoxifylline (PTX) on hypoxia-inducible factor-2 alpha (HIF-2α) and its role in controlling anemia in hemodialysis (HD) patients. Methods Eighty patients on HD were randomized to receive 400 mg of PTX or placebo twice daily for 6 months. The hemoglobin (Hb) and other hematologic parameters, the weekly erythropoiesis-stimulating agents (ESAs), and the ESA resistance index (ERI) were assessed monthly during the study. The HIF-2α, transforming growth factor-β1 (TGF-β1), and high-sensitivity C-reactive protein (hs.CRP) were measured before and after the intervention. Results In the pentoxifylline group, an appreciable increase in Hb from 9.7 (9.3, 10.3) g/dl to 10.5 (9.3, 11.4) g/dl and hematocrit (Hct) from 31.3 (29.6, 32.4)% to 33.2 (29.4, 35.9)% was observed after one month of PTX administration, and this effect was maintained over the study time (p < 0.001). This was along with a decrease in the ESA doses required from 8000 (8000, 11500) IU/wk to 4000 (2000, 8000) IU/wk (p < 0.001), and an improvement in the ERI from 11.6 (8.07, 16.97) IU/kg/wk/g/dl to 5.79 (2.01, 10.09) IU/kg/wk/g/dl (p < 0.001). Additionally, the HIF-2α increased significantly at the end of the intervention in patients who received PTX from 3245.35 (2886.8, 4691.56) pg/ml to 7208.75 (5382, 9182.7) pg/ml, while TGF-β1 and hs.CRP decreased significantly from 657.78 (539.78, 1146.62) pg/ml and 88.08 (39.93, 100.4) mg/l to 329.94 (228.67, 793.18) pg/ml and 48.65 (34.44, 84.61) mg/l, respectively. The percent changes in HIF-2α, TGF-β1, and hs.CRP levels in the pentoxifylline group were also statistically significant in comparison with the placebo group. Conclusions PTX could be a promising agent for correcting anemia in HD patients via increasing HIF-2α levels. Clinical trial registration Clinicaltrials.gov, February 2023, registry number: NCT05708248.

Ultrasound guided pediatric caudal dose: a two-center randomized controlled trial

BackgroundThe drug volume to be used in caudal in pediatric patients has remained an unmet issue since long. We determined the minimum drug volume required to reach T10 level in pediatric patients using ultrasonography and compared it with the already established volume by Armitage formula.AimTo determine the minimum effective caudal local anesthetic dose using ultrasound guidance.MethodsStudy was performed at two centres and at each centre, 50 pediatric patients (Total 100 patients), aged 1 to 3 years, undergoing below umbilical surgeries were included and randomised into two groups of 25 each (Total 50) to receive ultrasound guided drug volume vs. Armitage formula based volume. The volume required to reach T10 level was assessed with ultrasound in one group. Also, maximum height achieved, cutaneous level achieved after 15 min, FLACC scores 30 min post extubation and parental satisfaction scores were noted.ResultsThe mean drug volume required to reach T10 level in Group U was 0.755 ± 0.053 ml/kg with a P value < 0.001. (Compared to the drug volume of 1 ml/kg using one sample t test). The highest level achieved in both groups were calculated as the mode value of T8 and T7 in Group U and Group A respectively. The highest cutaneous level achieved after 15 min was also calculated as the mode value of T4 in both groups. FLACC scores at 30 min were also comparable. Satisfaction scores were comparable in both groups.ConclusionA volume of 0.7 ml/kg of local anaesthetic in pediatric caudal block is sufficient to achieve a target of T10 level for infraumblical surgeries.

Prickly Ash Seeds Improve the Ruminal Epithelial Development and Growth Performance of Hu Sheep by Modulating the Rumen Microbiota and Metabolome

It is known that the addition of feed rich in bioactive components to animal diets will affect rumen fermentation parameters and flora structure. However, research on the regulatory effects of prickly ash seeds (PASs) during rumen development or on the rumen microbiome and its metabolites in sheep is limited. The current study was designed to explore the effects of PASs on sheep rumen development and growth performance using metagenomics and metabolomics. Eighteen 3-month-old Hu lambs were randomly allotted to three different dietary treatment groups: 0% (basal diet, CK), 3% (CK with 3% PAS, low-dose PAS, LPS), and 6% (CK with 6% PAS, high-dose PAS, HPS) PASs. The lambs were slaughtered to evaluate production performance. Our results showed that dietary PAS addition improved the average daily gain and reduced the F/G ratio of the experimental animals. Additionally, the height and width of the rumen papilla in the treatment groups were significantly higher than those in the CK group. The fermentation parameters showed that the levels of acetate and butyrate were significantly higher in the LPS group than in the CK and HPS groups. The propionate levels in the HPS group were significantly higher than those in the CK and LPS groups. Metagenomics analysis revealed that PAS dietary supplementation improved the abundance of Clostridiales and Bacteroidales and reduced the abundance of Prevotella, Butyrivibrio, and Methanococcus. Metabolomic analyses revealed that increased metabolite levels, such as those of serotonin, L-isoleucine, and L-valine, were closely related to growth-related metabolic pathways. The correlations analyzed showed that papilla height and muscular thickness were positively and negatively correlated with serotonin and L-valine, respectively. Average daily gain (ADG) was positively and negatively correlated with L-valine and several Prevotella, respectively. In addition, muscular thickness was positively correlated with Sodaliphilus pleomorphus, four Prevotella strains, Sarcina_sp_DSM_11001, and Methanobrevibacter_thaueri. Overall, PAS addition improved sheep growth performance by regulating beneficial microorganism and metabolite abundances, facilitating bacterial and viral invasion resistance.

Reliability and utility of blood glucose levels in the periodontal pockets of patients with type 2 diabetes mellitus: a cross-sectional study

Background Several studies have measured gingival blood glucose (GBG) levels, but few have confirmed systematic bias using Bland–Altman analysis. This study compared the effectiveness of GBG levels with that of fingertip blood glucose (FTBG) levels using Bland–Altman and receiver operating characteristic (ROC) analyses. Methods A total of 15 healthy volunteers and 15 patients with type 2 diabetes were selected according to inclusion and exclusion criteria. Each group comprised eight male and seven female participants. The GBG and FTBG levels were measured using a self-monitoring blood glucose device after periodontal examination. Pearson’s product‒moment correlation and simple linear regression analyses were performed. In addition, Bland‒Altman analysis was also performed to assess the degree of agreement between the two methods. ROC analysis was conducted to determine the sensitivity, specificity, and cutoff values for patients with diabetes. The area under the ROC curve (AUC) was used to identify significant differences. Results The mean GBG and FTBG levels were 120 ± 44.8 mg/dL and 137 ± 45.1, respectively, for the whole sample. The mean GBG and FTBG levels were 145 ± 47.2 mg/dL and 163 ± 49.1, respectively, in the diabetes group. The mean GBG and FTBG levels in the nondiabetes group were 95.3 ± 25.2 and 111 ± 18.8, respectively. Patients with diabetes were more likely to have a probing pocket depth (PPD) of ≥4 mm at the sampled site. Pearson’s product‒moment correlation and simple linear regression analyses revealed a significant correlation between the GBG and FTBG measurements. Bland–Altman analysis revealed that GBG and FTBG measurements differed significantly among all participants; however, no significant differences were observed among the patients with diabetes (mean difference (MD) ± standard deviation (SD) = −18.1 ± 34.2, 95% confidence interval (CI) [−37.0 to 0.88]) or among the participants with a PPD of ≥4 mm (MD ± SD = −15.2 ± 30.4, 95% CI [−30.8 to 0.43]). The sensitivity, specificity, and cutoff values of the GBG measurements for detecting diabetes were 80%, 93%, and 123.5 mg/dL, respectively. The sensitivity, specificity, and cutoff values of the FTBG measurements for detecting diabetes were 73%, 87%, and 134.0 mg/dL, respectively. No significant differences were observed between the AUCs (0.078, 95% CI [−0.006 to 0.161]). Conclusions The GBG measurements aligned with the FTBG measurements in the patients with diabetes and among the participants with a PPD of ≥4 mm. Patients with diabetes were more likely to have a PPD of ≥4 mm at the sampled site, GBG levels can be used to screen for type 2 diabetes in dental clinics.

How the window of visibility varies around polar angle.

Contrast sensitivity, the amount of contrast required to discriminate an object, depends on spatial frequency (SF). The contrast sensitivity function (CSF) peaks at intermediate SFs and drops at other SFs. The CSF varies from foveal to peripheral vision, but only a couple of studies have assessed how the CSF changes with polar angle of the visual field. For many visual dimensions, sensitivity is better along the horizontal than the vertical meridian and at the lower than the upper vertical meridian, yielding polar angle asymmetries. Here, for the first time, to our knowledge, we investigate CSF attributes around polar angle at both group and individual levels and examine the relations in CSFs across locations and individual observers. To do so, we used hierarchical Bayesian modeling, which enables precise estimation of CSF parameters. At the group level, maximum contrast sensitivity and the SF at which the sensitivity peaks are higher at the horizontal than vertical meridian and at the lower than the upper vertical meridian. By analyzing the covariance across observers (n = 28), we found that, at the individual level, CSF attributes (e.g., maximum sensitivity) across locations are highly correlated. This correlation indicates that, although the CSFs differ across locations, the CSF at one location is predictive of that at another location. Within each location, the CSF attributes covary, indicating that CSFs across individuals vary in a consistent manner (e.g., as maximum sensitivity increases, so does the corresponding SF), but more so at the horizontal than the vertical meridian locations. These results show similarities and uncover some critical polar angle differences across locations and individuals, suggesting that the CSF should not be generalized across isoeccentric locations around the visual field.

Managing knowledge and sustainability in resource constrained disaster environments

AbstractOrganizations use both tangible and intangible resources for their existence to achieve sustainable development goals. Organizational knowledge mainly comprises of tacit knowledge held by employees. It becomes more critical when the situations are unpredictable, for example in rescue/disaster projects involving human lives at risk. Tacit knowledge utilization has been understudied particularly in such environments bounded by resource constraint and sustainability issues. This qualitative research attempts to fill this gap by collecting data from the knowledge workers in a large public sector non‐profit rescue organization. The study reveals various individual level, group level, organizational level, community level, and situational level factors, which impact the tacit knowledge utilization of rescue workers to properly execute emergency life threatening rescue operations and sustainability issues.

Early behavioral indicators of aberrant feces in newly-weaned piglets.

Post-weaning diarrhea (PWD) is a frequently occurring health and welfare issue in weaned piglets. Behavioral changes indicating impaired health may be detectable before the onset of signs and could be useful to detect the development of PWD early, enabling targeted and timely interventions. Current algorithms enable automated behavioral classification on the group level, while PWD may not affect all piglets in one pen and individual level analysis may be required. Therefore, this study aimed to assess whether changes in pen activity or individual piglet behavior can be early indicators of the occurrence of PWD. During 3 replicated rounds, 72 piglets (Sus scrofa domestica, Landrace x Large White) weaned at 27days of age, were housed in 4 pens with 6 piglets each. Individual fecal color and consistency were scored (0-5; ≥ 3 considered as aberrant feces) six times during the first two weeks post-weaning using rectal swabs. Additionally, using a similar scoring scale, feces on the pen floor were assessed daily. Two methods were applied for behavioral scoring. Individual behaviors (eating, drinking, standing, walking; n = 48) were scored manually and instantaneously with a five-minute interval from videos of the first two rounds, while pen activity (eating, drinking, moving; n = 12) was analyzed automatically and continuously using a commercially available algorithm from videos of all three rounds. Piglets showing a relatively higher proportion of standing behavior one day before fecal scoring had increased odds of an aberrant fecal color score (odds ratio (OR): 4.8; 95% confidence interval (CI): 1.5-15.3). Furthermore, odds of aberrant colored feces increased in pens where piglets showed more moving activity two days before (OR: 6.14; 1.26 < 95%CI < 29.84), which was also found for fecal consistency (OR: 4.77; 95%CI: 1.1-21.6). Our results indicate that increased standing in individual piglets and an increased moving activity on the pen level may be important behavioral indicators of PWD before the onset of diarrhea. Further development of current algorithms that can identify behavioral abnormalities in groups, from the pen to the individual level, may therefore be a promising avenue for improved and targeted health and welfare monitoring.

Safety and Immunogenicity of Intranasal Razi Cov Pars as a COVID-19 Booster Vaccine in Adults: Promising Results from a Groundbreaking Clinical Trial

Protective antibodies in the upper respiratory tract prevent the spread of COVID-19 in the community. Intranasal vaccines could raise the specific secretory IgA and IgG levels. This is a single-center, randomized, double-blind, placebo-controlled clinical trial to evaluate the safety and immunogenicity of Razi Cov Pars (RCP) intranasal recombinant protein subunit COVID-19 vaccine as a booster in adults. We compared specific IgG and IgA levels in the intranasal RCP group (n = 97) versus placebo (n = 96) in serum, saliva, and nasal mucosal secretions on days 0 and 14 and reported their Geometric Mean Ratios (GMR) and 95% confidence intervals (CI). We showed significant increases in IgA and IgG anti-RBD in the nasal mucosa in the RCP group, but their increase was not detectable in the serum and saliva. Anti-spike IgA in the nasal mucosa also increased in the RCP group compared to the placebo. This increase against the COVID-19 variant Omicron was also similar to that of the Wuhan. We detected no serious adverse reactions or anaphylaxis and all adverse events resolved completely during the follow-up period and were similar in both groups. Intranasal RCP is safe, stimulates the respiratory mucosal immunity, and could be a booster on various COVID-19 vaccines and be effective against new virus variants.