Lepus Timidus Research Articles

Complete Mitogenomes of Xinjiang Hares and Their Selective Pressure Considerations

Comparative analysis based on the mitogenomes of hares in Xinjiang, China, is limited. In this study, the complete mitochondrial genomes of seven hare samples including four hare species and their hybrids from different environments were sequenced, assembled, and annotated. Subsequently, we performed base content and bias analysis, tRNA analysis, phylogenetic analysis, and amino acid sequence analysis of the annotated genes to understand their characteristics and phylogenetic relationship. Their mitogenomes are circular molecules (from 16,691 to 17,598 bp) containing 13 protein-coding genes, two rRNA genes, 22 tRNA genes, and a control region, which are similar with other Lepus spp. worldwide. The relative synonymous codon usage analysis revealed that the adaptation of Lepus yarkandensis to its unique arid and hot environment might be associated with synthesizing amino acids like alanine, leucine, serine, arginine, and isoleucine and the terminator caused by the different usage of codons. Further, we utilized the MEME model and identified two positive selection genes (ND4, ND5) in Lepus tibetanus pamirensis and one (ND5) in L. yarkandensis that might be important to their adaptation to the plateau and dry and hot basin environments, respectively. Meanwhile, Lepus tolai lehmanni and Lepus timidus may have evolved different adaptive mechanisms for the same cold environment. This study explored the evolutionary dynamics of Xinjiang hares’ mitochondrial genomes, providing significant support for future research into their adaptation mechanisms in extreme environments.

Resources of mountain hare (Lepustimidus) in the Tver region

Mountain hare (Lepustimidus) is one of the most common game animals in the Tver region and a popular object of unprofessional hunting. The purpose of the research was the ecological and economic evaluation of the allocation of mountain hare resources in the Tver region for the period 2013–2022. Interval variation series were constructed to grade the indicators of the state of the species’ resources. For the fi rst time, maps were created that demonstrate the average long-term indicators of the abundance, production, population density, bioproductivity of hunting grounds and the output of mountain hare, as well as the cost assessment of the resources of this animal, taking into account the administrative-territorial division of the Tver region. During the analyzed period, fl uctuations in the number of mountain hares in the region were observed with an increase (decrease) in the indicator in relation to the previous year from –37.5 to 40.5 %. The situation with the volumes of production of this fur animal was unstable because of the resources were developed at the level of 6–17 % of the commercial population. The following average long-term indicators of the resource potential of mountain hare were obtained for the Tver region such as population 38 995 individuals, production volumes 3639 individuals, population density 5 individuals/1000 ha, resource cost 156 million rubles, bioproductivity of hunting grounds 5 rubles/ha, product output 0.5 rubles/ha. It was revealed that the Andreapolsky, Toropetsky and Udomelsky districts of the Tver region are the most promising for organizing unprofessional hunting for mountain hare.

Diet niches of the Alpine mountain hare (Lepus timidus varronis) and European hare (Lepus europaeus) living in coexistence in the Alps

Diet niches of the Alpine mountain hare (Lepus timidus varronis) and European hare (Lepus europaeus) living in coexistence in the Alps

Wildfire influences species assemblage and habitat utilisation of boreal wildlife after more than a decade in northern Sweden

Fires can strongly change the vegetation structure and the availability of resources for wildlife, but fire suppression has long affected the natural role of fire in shaping boreal ecosystems in northern Europe. Recently, wildfires have increased in frequency, possibly due to global warming. In contrast to the boreal systems in North America, there have been few studies on responses of wildlife to wildfires in northern Europe. Based on the findings from North America, we predict that responses of wildlife to wildfire vary among wildlife species: where mammalian herbivores, such as moose Alces alces and mountain hare Lepus timidus, will be attracted to burnt areas following an increase in food availability, other species, such as reindeer Rangifer tarandus, are negatively impacted due to fire reducing their preferred food. We then tested our predictions by contrasting wildlife utilization of sites that burnt by wildfire in 2006 with nearby unburnt control sites in three areas in northern Sweden. To measure wildlife utilization, we used 72 camera traps, equally divided between the burnt and control sites, with two placement strategies: random and on wildlife trails. The cameras recorded 27 mammal and bird species during summer 2018. Species assemblage differed between burnt and control sites. Fieldfare Turdus pilaris used burnt sites more than control sites, while pine marten Martes martes and western capercaillie Tetrao urogallus used control sites more than burnt sites. We however did not find support for a positive effect of past forest fires on any of the observed wild mammals. We discuss how, due to the impact of forestry, forage‐rich habitat may not be as limiting in Scandinavia as in the North‐American context, potentially leading to recently burnt sites being less attractive to herbivores such as moose.

The hare syphilis agent is related to, but distinct from, the treponeme causing rabbit syphilis.

The treponemes infecting lagomorphs include Treponema paraluisleporidarum ecovar Cuniculus (TPeC) and ecovar Lepus (TPeL), infecting rabbits and hares, respectively. In this study, we described the first complete genome sequence of TPeL, isolate V3603-13, from an infected mountain hare (Lepus timidus) in Sweden. In addition, we determined 99.0% of the genome sequence of isolate V246-08 (also from an infected mountain hare, Sweden) and 31.7% of the genome sequence of isolate Z27 A77/78 (from a European hare, Lepus europeaus, The Netherlands). The TPeL V3603-13 genome had considerable gene synteny with the TPeC Cuniculi A genome and with the human pathogen T. pallidum, which causes syphilis (ssp. pallidum, TPA), yaws (ssp. pertenue, TPE) and endemic syphilis (ssp. endemicum, TEN). Compared to the TPeC Cuniculi A genome, TPeL V3603-13 contained four insertions and 11 deletions longer than three nucleotides (ranging between 6 and2,932 nts). In addition, there were 25 additional indels, from one to three nucleotides long, altogether spanning 36 nts. The number of single nucleotide variants (SNVs) between TPeC Cuniculi A and TPeL V3603-13 were represented by 309 nucleotide differences. Major proteome coding differences between TPeL and TPeC were found in the tpr gene family, and (predicted) genes coding for outer membrane proteins, suggesting that these components are essential for host adaptation in lagomorph syphilis. The phylogeny revealed that the TPeL sample from the European brown hare was more distantly related to TPeC Cuniculi A than V3603-13 and V246-08.

Competition between sympatric hare species in the Alps is boostered by climate change and hybridisation

Climatic variation along the elevation gradient promotes the natural parapatric occurrence of the European hare (Lepus europaeus) and Alpine mountain hare (Lepus timidus varronis) in the Alps. Recent data indicate a displacement of mountain hares caused by competition with the European hare. Competitive exclusion might take place at a fine spatial scale and hybrids may sharpen competition. Genetic non-invasive sampling (gNIS) demonstrates to be effective to retrieve information from wild animals. However, based on the accuracy of the differing genetic analysis methods, the selection of the method might decisively influence results. To examine habitat preferences of Alpine mountain hares, European hares and their hybrids with particular interest in the influence of the accuracy of the genetic analysis method on the results, we performed gNIS in Grisons (Switzerland) for four years and compared habitat associations of the genotyped samples. We recorded 137 individuals (i.e., 35 hybrids, 49 European hares, 53 Alpine mountain hares). Combined nuclear and mitochondrial DNA analysis including individual identification revealed to be the most accurate indirect method for the study of habitat preferences of hares. Alpine mountain hares had a narrow habitat breadth and used little habitat diversity. Hybrids showed great similarities in their habitat preferences to European hares. Hybrids might increase the competition in favour of European hares and the displacement of Alpine mountain hares, since they show similar patterns of habitat use to European hares. Ongoing climate change potentiate the niche overlap between species, increasing the risk of Alpine hare decline due to hybridisation and displacement.

Scottish mountain hares do not respond behaviorally to camouflage mismatch

Scottish mountain hares do not respond behaviorally to camouflage mismatch

The distribution of mountain hares and the possible effects of woodland expansion using the Cairngorm National Park as a case study

The distribution of mountain hares and the possible effects of woodland expansion using the Cairngorm National Park as a case study

Species-specific variation in mitochondrial genome tandem repeat polymorphisms in hares (Lepus spp., Lagomorpha, Leporidae) provides insight into their evolution

The non-coding regions of the mitochondrial DNAs (mtDNAs) of hares, rabbits, and pikas (Lagomorpha) contain short (∼20 bp) and long (130–160 bp) tandem repeats, absent in related mammalian orders. In the presented study, we provide in-depth analysis for mountain hare (Lepus timidus) and brown hare (L. europaeus) mtDNA non-coding regions, together with a species- and population-level analysis of tandem repeat variation. Mountain hare short tandem repeats (SRs) as well as other analyzed hare species consist of two conserved 10 bp motifs, with only brown hares exhibiting a single, more variable motif. Long tandem repeats (LRs) also differ in sequence and copy number between species. Mountain hares have four to seven LRs, median value five, while brown hares exhibit five to nine LRs, median value six. Interestingly, introgressed mountain hare mtDNA in brown hares obtained an intermediate LR length distribution, with median copy number being the same as with conspecific brown hare mtDNA. In contrast, transfer of brown hare mtDNA into cultured mtDNA-less mountain hare cells maintained the original LR number, whereas the reciprocal transfer caused copy number instability, suggesting that cellular environment rather than the nuclear genomic background plays a role in the LR maintenance. Due to their dynamic nature and separation from other known conserved sequence elements on the non-coding region of hare mitochondrial genomes, the tandem repeat elements likely to represent signatures of ancient genetic rearrangements. clarifying the nature and dynamics of these rearrangements may shed light on the possible role of NCR repeated elements in mitochondria and in species evolution.

Dublin Hareport: The movement ecology and airfield interactions of resident, airside hares, at an international airport.

Understanding how animals move and use space within an environment is vital for the development and implementation of effective management actions. Within airfield environments, animal movement can present a substantial risk to aircraft, resulting in wildlife-aircraft collisions (strikes) if animals enter into the manoeuvring areas of the airfield, namely the runways, taxiways and areas that connect the two (hereafter collectively referred to as 'tarmacked areas'). However, reliable ecological data to inform management decisions can be difficult to obtain in such environments, due to access restrictions. Here, we present the first GPS data describing the movement ecology and spatial use of mammals on an airfield - Irish hare (Lepus timidus hibernicus), at Dublin International Airport - through the deployment of five GPS collars. A total of 4571 tarmacked area interactions were recorded between December 2021 and August 2022, with all five hares engaging with tarmacked areas. Between December and August, the highest number of interactions were recorded for the month of April (n = 1073), followed by March (n = 703). There was a mean of 4.3 (range: 0-65) interactions with tarmacked areas, per hare, per day throughout the study period. Hares most frequently engaged with tarmacked areas between 05:00 and 07:59, with some seasonal variation. The greatest cumulative distance moved per month was observed in May (505 km) and April (503 km). We identified that the average home range size of collared hares was 2.8 km2 (±SD 0.1 km2), based on 95% Kernel Utilisation Distribution. Furthermore, we demonstrate that the hares incorporate tarmacked area habitat types into their home ranges with up to 13% of one individual's movements incorporating these areas. Our study demonstrates the suitability of GPS tracking devices for studying the movement ecology of high-risk mammal species at airfields in order to inform airside management practices.

The impact of lead, cadmium, and mercury on the reproduction of mountain hares (Lepus timidus L., 1758) in the north of Krasnoyarsk Krai

Reproduction is key to the survival and development of a species. Anthropogenic activities release significant amounts of toxic pollutants into the environment. In this study, we aimed to determine effects of heavy metals on some reproductive parameters of the mountain hare. Female mountain hares (n = 41) were hunted in the reference and industrially polluted areas of Krasnoyarsk Krai during four seasons. Their skeletal muscles, liver, and kidneys were subjected to atomic absorption spectrometry to determine concentrations of lead, cadmium, and mercury. The contents of lead, cadmium, and mercury were significantly higher in the hares from the contaminated areas compared to the reference sites. According to the results, the exposure to lead, cadmium, and mercury had an impact on the reproductive potential of the female mountain hares. In particular, we established correlations between numbers of embryos and corpora lutea and contents of lead in the kidneys and liver, as well as cadmium in the kidneys. The number of corpora lutea and embryonic losses in the female hares from the contaminated areas were higher than those in the hared from reference areas. However, the numbers of embryos did not differ significantly between the compared areas. Our study showed that about 40% of the liver samples and 100% of the muscle tissue samples obtained from the hares in the impact zone contained high concentrations of lead and cadmium. Therefore, hunting in these industrially polluted areas may pose a toxic hazard to the indigenous peoples living there. Further research is needed to assess potential and actual fertility, offspring survival, and other important parameters of mountain hare populations exposed to different levels of chemical pollution.

New data on the ecology of the fossil don hare (<i>Lepus tanaiticus</i> [Gureev, 1964

A spore-pollen research of the contents of the gastrointestinal tracts and sediments containing frozen mummies of the fossil Don hare, discovered in the sediments of the Upper Pleistocene ice complex in the Verkhoyansk region of Yakutia, was carried out for the first time. Radiocarbon dating (C14) revealed that the found hares lived during the Karginian Interstadial of the Late Pleistocene, 32.5 thousand years ago (calibrated date). As a result, the understanding of the extinct Lepus tanaiticus ecology has been expanded: this species lived in cold steppes dominated by xerophytic communities, as well as in grass-forb and sedge-forb meadows; unlike the modern mountain hare L. timidus, which feeds during winter time mainly on branches and bark of woody plants and shrubs, the diet of Don hares in winter previously consisted of herbaceous plants.

The impact of tourist destinations on wildlife in northern Finland

ABSTRACT Increased tourism impact, recreational activities and structures in natural areas can affect wildlife by increasing urbanization, landscape fragmentation and disturbance. I examined the potential effects of tourist destinations on four forest grouse (Tetraoninae) and five mammalian species in northern Finland. I analyzed density data obtained from wildlife counts carried out by hunters around ten tourist destinations. The densities of mountain hare and mustelid species were negatively correlated with the distance to a tourist destination. The densities of adult grouse, juvenile grouse, mountain hare, and mustelids were positively correlated with the area of mixed forests surrounding the destinations. The densities of adult and juvenile grouse were positively correlated, while the densities of pine marten and mustelids were negatively correlated with the area of agricultural land surrounding the destinations. The densities of the studied wildlife species varied among destinations and years. It seems that current recreational activities have not caused significant changes in the occurrence and abundance of wildlife species in the surroundings of the tourist destinations studied. Location of a destination, predator densities, and landscape structure around the destination had the most impact on the density of wildlife species.

Population trend and distribution of mountain (Lepus timidus) and brown hares (Lepus europaeus) in Central Alps (N-Italy, 1980–2020)

Population trend and distribution of mountain (Lepus timidus) and brown hares (Lepus europaeus) in Central Alps (N-Italy, 1980–2020)

Camera traps reveal seasonal variation in activity and occupancy of the Alpine mountain hare Lepus timidus varronis

Mountain hare is a cold‐adapted species threatened by climate change, but despite its emblematic nature, our understanding of the causes of population decline remains limited. Camera traps are increasingly used in ecology as a tool for monitoring animal populations at large spatial and temporal scales. In mountain environments where field work is constrained by difficult access and harsh conditions, camera traps constitute a promising tool for surveying rare and elusive species such as the mountain hare. Our study explored the use of camera traps as a tool for studying seasonal habitat occupancy and daily activity patterns of the mountain hare, in order to carry out long‐term monitoring of populations. We installed 46 camera traps along elevation gradients in the Mont‐Blanc massif (France) from January 2018 to June 2022. We measured habitat variables at each camera trap site in order to define vegetation composition and habitat structure. We performed multi‐season and single‐season occupancy models to respectively describe habitat occupancy of the mountain hare throughout the year and identify the environmental variables influencing mountain hare presence during the breeding season. Mountain hares occupy coniferous forest in winter, and then switch to mixed areas of shrubland and grassland above treeline in spring and the beginning of summer. In spring, occupancy probability of the mountain hare increases with relative cover of mixed low shrub and herbaceous layer (i.e. the 10–40 cm vegetation layer), suggesting a link to food resources and protection from predation. Our results also confirm the nocturnal and crepuscular activity of the mountain hare during the breeding season, and strictly nocturnal activity in winter. Our results demonstrate the efficiency of camera traps as tools for monitoring mountain hare habitat occupancy in mountain environments and underline the importance of diverse habitat mosaics for the preservation of the species.

The functional role of mammals in the restoration of disturbed taiga ecosystems of the Vologda Region

The article examines the participation of mammals in the restoration of disturbed ecosystems. Since in a number of regions of the North-West the intensity of anthropogenic load in the form of logging activities does not decrease, and in some cases even increases, the study of the degree of influence of each component of the ecosystem on the restoration of disturbed taiga biogeocoenoses is an urgent problem and requires comprehensive consideration. In the Vologda Oblast, logging activities are aimed at increasing the area of continuous concentrated logging, which have a serious impact on forest biogeocoenoses. Environment-forming (environment-forming or biogeocoenotic activity) animals are the work performed by organisms to implement and regulate any biogeocoenotic processes, in particular, to transform substances and energy, and maintain the equilibrium state of the system. The article considers the most pronounced types of environmental and biogeocoenotic activity of terrestrial mammals – trophic-mechanical (using the example of elk and white hare), excretory (elk, white hare, wild boar, bear, wolf and fox), hard-laying (mole) and digging-loosening (wild boar) in the territories disturbed after logging activities taiga mesoecosystems (logging). It was revealed that as a result of trophomechanical activity, phytophages do not damage the woody phytocoenosis, since the mosaic of habitat and successional processes towards deciduous plants – rowan, aspen and willow, provides phytophagous mammals with food in sufficient quantities. Excretory, excretory-recomposing and digging-loosening activities of mammals support the process of circulation of substances and the flow of energy, which contributes to self-healing, self-purification, self-regulation and increased stability of disturbed forest taiga ecosystems of Vologda Region.

Diet Composition Explains Interannual Fluctuations in Reproductive Performance in a Lowland Golden Eagle Population

Food supply stands out as one of the most critical drivers of population demographics by limiting reproductive rates. In this study, we assessed fluctuations in diet composition and monitored various reproductive parameters over a nine-year period in a Golden Eagle population in an Eastern European peatland. The identification of 2439 prey specimens from 84 species revealed that the studied eagles primarily preyed upon birds (constituting 78.3% of prey numbers and 67.2% of prey biomass) and less on mammals (21.6% and 32.8%, respectively). Grouse emerged as the most important prey group (31% and 27%), followed by waterfowl (17%) and hares (8% and 14%). The most significant prey species, both in terms of numbers and weight, were the Black Grouse, Mountain Hare, Common Crane, and Capercaillie. The share of the Black Grouse decreased, while those of the White-fronted Goose, Roe Deer, and hares increased. The food niche, as measured by the Levins’ index, was broad (6.6), and it expanded during the study. On average, 58.3% of Golden Eagle pairs initiated breeding annually, with 69.1% successfully completing it, and 0.41 young per occupied territory were produced annually; there was pronounced interannual variation in reproductive performance. These fluctuations were associated with the shares of White-fronted Goose, Capercaillie, Mountain Hare and Roe Deer in the diet, suggesting that these species may be gaining increasing importance for the Golden Eagle, particularly following the decline of the Black Grouse.

Taxonomic status of hares (Lepus spp.) in Xinjiang, China (Lagomorpha: Leporidae): An integrative approach

AbstractThe taxonomic status and interspecies relationships of hares (genus Lepus) in Xinjiang, China, remain ambiguous owing to extremely similar external morphologies, introgressive hybridization between species and a lack of molecular genetic records. In this study, a total of 154 hare specimens were used to clarify their taxonomic status. Based on multiple lines of evidence, including observations and comparisons of external morphological characteristics, skull morphometrics and genome‐wide single nucleotide polymorphism (SNP) datasets, four hare species were recognised. Lepus yarkandensis and Lepus timidus, which have obvious morphological characteristics, were identified as two distinct species, as previously reported. Combining the integrated morphological and molecular datasets with the relevant literature, we proposed suggestions on the classification of the more controversial hares. Due to molecular and morphological similarities, hares in northern and central Xinjiang were divided into two subspecies of Lepus tolai, namely, Lepus tolai lehmanni and Lepus tolai centrasiaticus. Despite similar external morphological characters, hares in the southwest Pamir Plateau and its surroundings can be distinguished from L. tolai by differences in skull morphology and genetic divergence and were, therefore, considered a subspecies of Lepus tibetanus, Lepus tibetanus pamirensis. Furthermore, we found that hybridization and introgression occurred between sympatric or parapatric hares, especially between L. yarkandensis and L. tibetanus pamirensis, L. yarkandensis and L. tolai centrasiaticus, and L. tolai lehmanni and L. timidus.

Mammal inventory using camera traps in the Central Forest State Nature Reserve (West of European Russia)

A mammal species inventory by estimating their richness is the first and necessary task of any mammal monitoring programme. This paper presents estimates of species richness of mammals (mostly medium and large body-sized) in the Central Forest State Nature Reserve (European Russia). Based on data collected in 2023 from 55 camera trap locations (19 676 camera days), 59 035 images of wild mammals were obtained. A total of 18 species were recorded. Of these, ten species were representatives of the Carnivora, four – Artiodactyla, two – Rodentia, one – Erinaceomorpha, and one – Lagomorpha. This amounted to 84% of all potentially camera-trapped mammals observed in the Central Forest State Nature Reserve. The relative abundance index (RAI) and naïve occupancy (ψnaive) were calculated for all species. The most common and frequently recorded were Meles meles (RAI = 4.95; ψnaive = 0.96), followed by Ursus arctos (RAI = 4.39; ψnaive = 1) and Lepus timidus (RAI = 4.08; ψnaive = 1), while the most rarely recorded species were Castor fiber (RAI = 0.01; ψnaive = 0.02), Erinaceus europaeus (RAI = 0.02; ψnaive = 0.04), and Lutra lutra (RAI = 0.03; ψnaive = 0.07). Large and medium-sized carnivorous and omnivorous mammal communities were the fastest and most completely captured. According to the constructed single-season multispecies occupancy model with Markov chains Monte Carlo, camera traps failed to record 4–5 more possible species. These species are likely to be Mustela lutreola, M. erminea, and Mustela nivalis, as well as two invasive species, Cervus nippon and Capreolus pygargus. All the captured species were recorded in total in a short period of time, namely from the end of March to the beginning of May during 1869 camera-days. Two of them (Castor fiber and Erinaceus europaeus) were recorded exclusively at this time, and they were not captured further until the end of the year. This emphasises the importance of conducting mammal inventory, with inclusion of the spring season.

Abundance of Eurasian lynx in the mountain taiga of the Southern Urals

The study aims to analyze the dynamics of the Eurasian lynx in the mountain taiga, and to search for the factors affecting the numbers of this predator. The study was conducted in the Southern Urals (Russia) from 2008 to 2019. The dynamics of lynx and its main prey numbers Capreolus pygargus, Lepus timidus and L. europaeus, Tetrao urogallus and Bonasa bonasia is based on the data obtained during the complex winter census (CWC). Annual CWCs were performed in Chelyabinsk Oblast with total area of 23.445.5 km². The hypotheses on the influence of snow depth on the number of lynx and the relationship between the dynamics of the predator and prey abundances were tested using regression method. The relationship between the number of lynx and snow depth were obtained using Spearman's coefficient. The number of lynxes varied from 51 to 281 individuals, population density - from 0.09 lynx/100 km2 to 0.48 lynx/100 km2 . The lowest abundances were recorded in 2013 and 2016. In the winters of 2013 and 2016, the snow depth was 2.0-2.5 times greater than on average for long-term observations. However, no significant decrease in the number of the Siberian roe deer was observed in these years, while the number of lynxes decreased by 2 times compared to the previous years. No significant correlation was found between the abundance of lynxes and the Siberian roe deer, r = 0.25 ± 0.3. A negative relationship was found between the amount of precipitation and the winter number of lynxes (r = -0.717 ± 0.22; p ˂ 0.05). The amount of snow cover in the Southern Ural Mountains is an important factor affecting the distribution of ungulates. The snow depth may determine the lynx presence both directly and through the changes in the number of its main prey.