Scan Lens Research Articles

Off-the-shelf optical systems design enabled by an evolution strategy: front stop case

Commercial off-the-shelf optics enable economic and rapid solutions in the photonics industry and academia. However, the design of optical systems with off-the-shelf optics is a time-consuming task for experienced optical designers and hopeless for novice designers. In this paper, we propose an automatic optical design tool to generate optical systems using only off-the-shelf optical components without human assistance. Our solution is based on an evolution strategy (ES) that performs a discrete combinatorial optimization following optical design-based methodologies that satisfy user-defined specifications. Unlike the conventional methods, the algorithm decreases the design process time and provides optical designers with several optical solutions from where to choose and adapt for targeted applications. In this work, the ES is described and tested with front stop optical configurations. We demonstrate the broad solution domain of the algorithm through the generation of optical systems with F-numbers within a range F/1 to F/90 and field of views up to 300 mm at the image plane. To analyze the solution domain and the characteristics of the solution, we used the design specifications of 29 commercially available scan lenses and compared the performance of different ES parameters. The compatibility of our algorithm with (standard) commercially available optical design software unlocks automatic design tools for off-the-shelf optical systems.

Multiplex fluorescence detection of single-cell droplet microfluidics and its application in quantifying protein expression levels.

This study presented a platform of multiplex fluorescence detection of single-cell droplet microfluidics with demonstrative applications in quantifying protein expression levels. The platform of multiplex fluorescence detection mainly included optical paths adopted from conventional microscopy enabling the generation of three optical spots from three laser sources for multiple fluorescence excitation and capture of multiple fluorescence signals by four photomultiplier tubes. As to platform characterization, microscopic images of three optical spots were obtained where clear Gaussian distributions of intensities without skewness confirmed the functionality of the scanning lens, while the controllable distances among three optical spots validated the functionality of fiber collimators and the reflector lens. As to demonstration, this platform was used to quantify single-cell protein expression within droplets where four-type protein expression of α-tubulin, Ras, c-Myc, and β-tubulin of CAL 27 (Ncell = 1921) vs WSU-HN6 (Ncell = 1881) were quantitatively estimated, which were (2.85 ± 0.72) × 105 vs (4.83 ± 1.58) × 105, (3.69 ± 1.41) × 104 vs (5.07 ± 2.13) × 104, (5.90 ± 1.45) × 104 vs (9.57 ± 2.85) × 104, and (3.84 ± 1.28) × 105 vs (3.30 ± 1.10) × 105, respectively. Neural pattern recognition was utilized for the classification of cell types, achieving successful rates of 69.0% (α-tubulin), 75.4% (Ras), 89.1% (c-Myc), 65.8% (β-tubulin), and 99.1% in combination, validating the capability of this platform of multiplex fluorescence detection to quantify various types of single-cell proteins, which could provide comprehensive evaluations on cell status.

Miniaturized high-resolution dual 2D MEMS mirror scanning confocal Raman microscopy for topographic and Raman mapping

A novel dual 2D MEMS mirror scanning confocal Raman microscopy (D-2D-MEMS-CRM) method is proposed to realize the miniaturized design of the confocal Raman microscopy. This method uses two 2D MEMS mirrors to realize beam telecentric scanning, which eliminates the relay system consisting of a scan lens and tube lens, significantly shortens the optical length of the system, and improves the detection efficiency of Raman signal. Moreover, this method uses reflected light to construct a real-time focus tracking system, which improves the spatial resolution of the confocal Raman detection system. Based on this method, a miniaturized D-2D-MEMS-CRM system was established, which had an axial focusing capability of 50 nm using an objective lens with NA of 0.6, and the lateral resolution of 500 nm for both topographic and Raman mapping. In addition, the system achieved simultaneous topographic imaging and Raman mapping of carbonaceous chondrite, demonstrating its effectiveness in extraterrestrial sample detection.

Millimeter field-of-view miniature two-photon microscopy for brain imaging in freely moving mice.

Development of miniature two-photon microscopy (m2PM) has made it possible to observe fine structure and activity of neurons in the brain of freely moving animals. However, the imaging field-of-view of existing m2PM is still significantly smaller than that of miniature single-photon microscopy. Here we report that, through the design of low-magnification objective, large field-of-view scan lens and small tilt angle microscanner, a 2.5-g m2PM achieved a field-of-view of 1000 × 788 µm2, comparable to that of a typical single-photon miniscope. We demonstrated its capability by imaging neurons, dendrites and spines in the millimeter field-of-view, and simultaneous recording calcium activities, through a gradient-index lens, of approximately 400 neurons in the dorsal hippocampal CA1 in a freely moving mouse. Integrated with a detachable 1.2-g fast z-scanning module, it enables a 1000 × 788 × 500 µm3 volumetric neuronal imaging in the cerebral cortex. Thus, millimeter FOV m2PM provides a powerful tool for deciphering neuronal population dynamics in experimental paradigms allowing for animal's free movement.

Advances in CODE V design in terahertz imaging system

The band of terahertz (THz) spans from microwave to infrared bands, which belongs to the transition band from macro-electronics to micro-photonics, possessing peculiarities different from electromagnetic waves in other bands. THz technology with the characteristics of high penetration, broadband, high resolution and transient are employed in various fields, such as space remote sensing, nondestructive detection biomedical imaging and near-field microscopy. These broad applications, in turn, provide incentive to further improve the basic performance of THz technology. Aiming at the application of terahertz technology in the imaging field, this novel mainly discussed the basic principle, key devices, system composition, technical advantages and future development trend of terahertz imaging system. The application status of CODE V optical design software in designing scanning lens systems for terahertz imaging, such as free-form surfaces, non-free surfaces and multi-lens groups are further discussed in this paper.

Design and experimental demonstration of a beam scanning lens antenna.

A beam scanning antenna based on an all-metal lens is presented for high-power microwave (HPM) application in this paper. This design includes a feed antenna and two layers of different all-metal lenses, whose prototypes operating at 14.25GHz have been successfully designed and fabricated with an aperture radius of 300mm. A full transmission phase range of 360° can be achieved with a transmission efficiency of over 99% by rotating the cross-slot on the lens elements. The results of the low-power tests depict that the designed antenna can realize a beam scanning range of 120° revolving cone angle with the side lobe below -10 dB and the reflection coefficient less than -16 dB. The high-power tests demonstrate that the power handling capacity of the antenna is higher than 350MW in sulfur hexafluoride (SF6). In addition, both the designed lenses have a bandwidth of more than 100MHz. All the merits show that our designed all-metal lens antenna has a great potential to be applied in HPM systems.

Reconfigurable scan lens based on an actively controlled optical phased array.

Integrated photonics provides a path for miniaturization of an optical system to a compact chip scale and offers reconfigurability by the integration of active components. Here we report a chip-scale reconfigurable scan lens based on an optical phased array, consisting of 30 actively controlled elements on the InP integrated photonic platform. By configuring the phase shifters, we show scanning of a nearly diffraction-limited focused spot with a full width at half maximum spot size down to 2.7 µm at the wavelength of 1550 nm. We demonstrate the key functions needed for a laser-scanning microscope, including light focusing, collection, and steering. We also perform confocal measurements to detect reflection at selective depths.

First Demonstration of Dynamic High-Gain Beam Steering With a Scanning Lens Phased Array

We report on the first demonstration of dynamic beam steering using a scanning lens phased array. A scanning lens phased array relies on a combination of mechanical and electrical phase shifting to dynamically steer a high-gain beam beyond the grating-lobe free region using a sparse array. These two concepts have been demonstrated separately in the past, here we present, for the first time, a prototype demonstration where active mechanical and electrical phase shifting are combined. For this purpose, we have developed a sparse 4x1 scanning lens phased array at W-band (75-110 GHz) capable of beam steering a directive beam (>30 dBi) towards ± 20° with low grating lobe levels (around -10 dB). The lens array is fed by a waveguide-based leaky-wave feeding architecture that illuminates the lenses with high aperture efficiency over a wide bandwidth, which is required in the proposed scanning lens phased array architecture. The electrical phase shifting has been implemented using IQ-mixers around 15 GHz in combination with x6 multipliers to reach the W-band. The mechanical phase shifting relies on a piezo-electric motor, which is able to achieve displacements of the lens array of 6 mm with an accuracy of a few nanometers. The entire active array is calibrated over the air with an ad-hoc quasi-optical measurement setup. Resulting measurements show excellent agreement with the anticipated performance.

Effect of scanner lens on lateral response artefact in radiochromic film dosimetry

Radiochromic film is a good dosimeter choice for patient QA for complex treatment techniques because of its near tissue equivalency, high spatial resolution and established method of use. Commercial scanners are typically used for film dosimetry, with Epson scanners being the most common. Radiochromic film dosimetry is not straightforward having some well-defined problems which must be considered, one of the main ones being the Lateral Response Artefact (LRA) effect. Previous studies showed that the contributing factors to LRA are from the structure of the active ingredients of the film and the components and construction of the flatbed scanner. This study investigated the effect of the scanner lens on the LRA effect, as part of a wider investigation of scanner design effects and uncertainties. Gafchromic EBT3 films were irradiated with 40 × 40 cm2 field size 6 MV beams. Films were analysed using images captured by a Canon 7D camera utilising 18 mm, 50 mm and 100 mm focal length lenses compared to images scanned with a conventional Epson V700 scanner. The magnitude of the LRA was observed to be dependent on the focal length of the lens used to image the film. A substantial reduction in LRA was seen with the use of the 50 mm and 100 mm lenses, by factors of 3–5 for the 50 mm lens and 4–30 for the 100 mm lens compared to conventional desktop scanner techniques. This is expected to be from the longer focal length camera lens system being able to collect more light from distant areas compared to the scanner-based system. This provides an opportunity to design film dosimetry systems that minimise this artefact.

2D scanning SERS probe for early biofilm boundary determination

Surface-enhanced Raman scattering (SERS) enables the biofilm detection by the characteristic Raman spectrum of extracellular polymeric substance (EPS). However, its clinical application in wound biofilm delineation is hindered by long sampling time and unpredictable sampling errors. To overcome these limitations, we propose a two dimensional (2D) scanning SERS probe that combines the advantages of galvo mirrors and a custom designed SERS active substrate. In this design, the SERS active substrate with dendritic nano-silver structure is fixed on the focal plane of the scan lens to maintain not only the required distance of the sampling plane but also the stable sampling condition as the excitation laser beam driven by the galvo mirrors scans quickly over the region of interest (ROI). Our benchtop study shows that the ± 3.75 mm offset between the sampling plane and the focal plane results in less than 10% change in both laser spot size and fluctuation in Raman peak intensity for the 2D scanning SERS probe, superior to the commercial Raman probe where 1700% change in laser spot size and 95% fluctuation in Raman peak intensity. Our in vitro study in two typical biofilms shows that the 2D SERS scanning probe can detect the biofilm as early as 4 h of cultivation and delineate the biofilm boundary with accuracy at 8 h of cultivation. Furthermore, this substrate is disposable, antibacterial, and capable of enhancing Raman intensity with consistency. Our studies imply the clinical potential of using the 2D SERS scanning probe for biofilm delineation in chronic wound management.

Angular dependence of coaxial and quasi-coaxial monitoring systems for process radiation analysis in laser materials processing

Process monitoring is becoming increasingly important in laser-based manufacturing and is of particular importance in the field of additive manufacturing [e.g. Laser Powder Bed Fusion (LPBF)]. Process monitoring enables a reduction of production costs and a lower time-to-market. Furthermore, the data can be used to create a digital twin of the workpiece. There are already many established processing head-integrated monitoring systems for such applications as the multispectral analysis of process radiation. However, the monitoring of complex signals in systems with F-Theta scanner lenses is very challenging and requires specially adapted optics or measuring sensors.In this paper a potential arrangement for spectroscopy-based process monitoring in pre-objective scanning is presented. The process radiation was monitored using a coaxial and a quasi-coaxial observation system. The measurements were carried out on both a solid and a powder coated sample of 2.4668 (Inconel 718) to show the potential use of these systems in laser-based additive manufacturing. In order to obtain comprehensive data about the process signal, the process zone was analyzed at different angles of incidence (AOI) of the laser using a high-speed camera (HSI) and a spectrometer. The connection between the HSI and the spectral measurements is discussed. The ionization of the material and the formation of a plasma was observed and found to lose intensity as the angle of incidence increases. A model of the system that demonstrates the intensity of the emitted radiation of the plasma was created. It enables the measured values to be corrected. The corrected measurement data can be used to detect impurities or a non-ideal energy input across the entire processing field, which is a move towards robust process monitoring.

Supervised machine learning for automatic classification of in vivo scald and contact\xa0burn injuries using the terahertz Portable Handheld Spectral Reflection (PHASR) Scanner

We present an automatic classification strategy for early and accurate assessment of burn injuries using terahertz (THz) time-domain spectroscopic imaging. Burn injuries of different severity grades, representing superficial partial-thickness (SPT), deep partial-thickness (DPT), and full-thickness (FT) wounds, were created by a standardized porcine scald model. THz spectroscopic imaging was performed using our new fiber-coupled Portable HAndheld Spectral Reflection Scanner, incorporating a telecentric beam steering configuration and an f-theta scanning lens. ASynchronous Optical Sampling in a dual-fiber-laser THz spectrometer with 100 MHz repetition rate enabled high-speed spectroscopic measurements. Given twenty-four different samples composed of ten scald and ten contact burns and four healthy samples, supervised machine learning algorithms using THz-TDS spectra achieved areas under the receiver operating characteristic curves of 0.88, 0.93, and 0.93 when differentiating between SPT, DPT, and FT burns, respectively, as determined by independent histological assessments. These results show the potential utility of our new broadband THz PHASR Scanner for early and accurate triage of burn injuries.

Fast-printed, large-area and low-cost terahertz metasurface using laser-induced graphene

Terahertz (THz) technology has made promising progress and attracted wide interests in the fields of communications, security, agriculture, and biological sciences. However, the lack of modulation materials and the great challenge of the miniaturized system have become the huge restriction of this technology. Although artificial metamaterials have shown impressive electromagnetic performances in this band, they suffer complicated and high-cost fabrication processes. In this work, we find that due to the high conductivity, laser-induced graphene (LIG) can be applied in THz wave modulation. A LIG metasurface fabrication system is developed based on a nanosecond laser, a telecentric scan lens, and scanning mirrors. Owing to the ultra-small focal spot (≤5 μm) realized by the telecentric scan lens and the fast-scanning speed of the mirrors, a LIG-based THz metasurface with the size of 15 mm × 15 mm, and the resolution of 30 μm can be fabricated in 34 s. In addition, THz optical parameters of LIG are measured. It is anticipated that this fast-fabricating, large-area, and low-cost method may open up a brand-new way to develop THz devices.

Dichroic Scanning Nonlinear Optical Microscope With Photodetecting Polymer Scanner and Objective Lens With External Back Focal Plane

In this article authors present a prototype of a dichroic scanning nonlinear optical microscope with a photodetecting polymer Scanner and a customized objective lens with an external back focal plane. Two types of two-axis electromagnetic actuated polymer scanners are manufactured by a standard printed circuit board (PCB) fabrication process. Both scanners share the same overall dimensions of 45 mm by 45 mm. The maximum optical scan angle of a type I scanner is 13 degrees at 420 Hz by the fast axis in resonance and 4 degrees by the slow axis in the linear mode. The type II scanner shows similar performance. A scan lens as well as a tube lens in a conventional nonlinear optical microscope are replaced by placing such a polymer scanner on the external back focal plane of a customized water immersion objective lens. The objective lens has the back working distance of 6 mm, the numerical aperture of 0.7, the front working distance of 1.16 mm, a field of view of 1.67 mm in the diameter and the angle of view of +/−15 degrees. Preliminary imaging results are also presented.

Compact computational spectrometer using a solid wedged low finesse etalon.

A novel, to the best of our knowledge, two-layer hybrid solid wedged etalon was fabricated and combined with a traditional imager to make a compact computational spectrometer. The hybrid wedge, comprised of ${{\rm Nb}_2}{{\rm O}_5}$ and Infrasil 302, was designed to operate from 0.4-2.4 µm. Initial demonstrations, however, used a complementary metal-oxide semiconductor (CMOS) imager and demonstrated operation from 0.4-0.9 µm with spectral resolutions ${\lt}\;{30}\;{{\rm cm}^{- 1}}$ from single snapshots. The computational spectrometer itself operates similarly to a spatial Fourier transform spectrometer (FTIR), but rather than use conventional Fourier-based methods or assumptions, the spectral reconstruction used a non-negative least-squares fitting algorithm based on analytically computed wavelength response vectors determined from extracted physical thicknesses across the entire two-dimensional wedge. This new computational technique resulted in performance and spectral resolutions exceeding those that could be achieved from Fourier processing techniques applied to this wedge etalon. With an additional imaging lens and translational scanning, the system can be converted into a hyperspectral imager.

A Dual-Polarized 27 dBi Scanning Lens Phased Array Antenna for 5G Point-to-Point Communications

A dual-polarized 4×4 scanning phased array antenna with leaky-wave enhanced lenses operating at 28 GHz is presented. Such an antenna can be used for point-to-point fifth-generation (5G) communications that require high gain, wide bandwidth (BW), and limited steering ranges. The proposed array has a periodicity of two wavelengths, and the resulting grating lobes are suppressed by directive and steerable array element patterns. To achieve a low-cost and low-profile solution, the leaky-wave antenna feeds are designed in printed circuit board and the lenses are made of plastic. The lenses are optimized in the near-field region of the feeds, with the goal of maximizing the array element aperture efficiency. The array performance obtained from the proposed approach is validated by full-wave simulations, showing a 27.5 dBi broadside gain at 28 GHz and a steering capability up to ±20° with 2 dB of scan loss. An antenna prototype was fabricated and measured. Measurement results are in excellent agreement with full-wave simulations. The prototype antenna, at broadside, achieves a 20% relative BW and a gain of 26.2 dBi.

Design and characterization of telecentric f-θ scanning lenses for broadband terahertz frequency systems.

Telecentric beam scanning using f-θ lenses offers nearly uniform spot size, linear beam displacement, and normal incidence angle over a planar surface. These unique properties allow for the minimization of imaging distortion over a wide field-of-view. In this article, we present a numerical method for designing custom f-θ lenses in the THz regime. We fabricated three lenses made from different commonly used polymer materials in the THz optics. We demonstrated their optical performance metrics compared to a conventional plano-convex lens over the broadband 0.3 THz–1 THz range. We find that the f-θ lens designed using the optical properties of high-density polyethylene achieved superior performance by maintaining a constant phase over a wide field of view of about 34°. We demonstrate this isophase property by measuring a constant time of arrival of the THz time-domain pulses over a reference mirror with a standard deviation of ∼19 fs, in excellent agreement with simulation predictions. This work will pave the way for the design and implementation of highly precise and fast telecentric imaging systems in the THz frequencies.

Wideband Multimode Leaky-Wave Feed for Scanning Lens-Phased Array at Submillimeter Wavelengths

In this article, we propose a hybrid electromechanical scanning lens antenna array architecture suitable for the steering of highly directive beams at submillimeter wavelengths with field-of-views (FoV) of ±25°. The concept relies on combining electronic phase shifting of a sparse array with a mechanical translation of a lens array. The use of a sparse-phased array significantly simplifies the RF front-end (number of active components, routing, thermal problems), while the translation of a lens array steers the element patterns to angles off-broadside, reducing the impact of grating lobes over a wide FoV. The mechanical translation required for the lens array is also significantly reduced compared to a single large lens, leading to faster and low-power mechanical implementation. In order to achieve wide bandwidth and large steering angles, a novel leaky wave lens feed concept is also implemented. A 550-GHz prototype was fabricated and measured demonstrating the scanning capabilities of the embedded element pattern and the radiation performance of the leaky wave fed antenna.

Multimodal imaging with spectral-domain optical coherence tomography and photoacoustic remote sensing microscopy.

We develop a multimodal imaging platform, combining depth-resolved scattering contrast from spectral-domain optical coherence tomography (SD-OCT) with complementary, non-contact absorption contrast using photoacoustic remote sensing (PARS) microscopy. The system provides a widefield OCT mode using a telecentric scan lens, and a high-resolution, dual-contrast mode using a 0.26 numerical aperture apochromatic objective. An interlaced acquisition approach is used to achieve simultaneous, co-registered imaging. The SD-OCT modality provides a 9.7 µm axial resolution. Comprehensive in vivo imaging of a nude mouse ear is demonstrated, with the SD-OCT scattering intensity revealing dermal morphology, and PARS microscopy providing a map of microvasculature.

Terahertz time-domain spectral imaging using telecentric beam steering and an f-θscanning lens: distortion compensation and determination of resolution limits.

We report on the development and performance characterization of a telecentric terahertz spectroscopic scanner using an f-θ objective lens and a single gimballed scanning mirror for image formation. We derived a beam steering transform to compensate for the intercoupling of the gimballed mirror axes and the distortions caused by an imperfect scanning lens. We characterize the optical performance of the system in both the time and spatial domains, demonstrating a constant diffraction-limited imaging resolution over the entire field of view. Finally, given the large depth of focus of the objective lens, we demonstrate the broadband imaging capability at different depths using a Boehler star target. This imaging setup has the potential to be miniaturized into portable form factors for field-deployable scenarios.