The paper discussed the current research on the applicability of biosorbents for the purification of biogas, particularly the decrease of H2S by using encapsulated or embedded biological biomass. This study investigated the potential of alginate-yeast biosorbent (AlgY) for biogas purification, focusing on hydrogen sulfide (H2S) removal. A biogas column test was conducted to compare the biosorption efficiency of AlgY and pure alginate beads. Using Response Surface Methodology (RSM), the effects of column length, acquisition time, and biosorbent type were evaluated for CH4, CO2, and H2S removal. Results depicted significant H2S reduction, with AlgY achieving a p-value of < 0.0001 and a high correlation coefficient (R2 = 0.9518). The relatively high correlation coefficient (R2) of the tested quadratic model of all the responses were recorded (R2; 0.5560, 0.5048, and 0.9518 for CH4, CO2, and H2S respectively). According to the studies’ preliminary findings, the type of biosorbent has a significant role in determining the biosorption effectiveness. The ANOVA of model terms depicted a significant p-value (p < 0.05) indicated a potential alginate-yeast (AlgY) biosorbent for H2S purification or reduction.
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