Length-dependent Effect Research Articles

Small fatigue crack behavior of CP-Ti in thin-walled cruciform specimens under biaxial loading

This study investigates the small fatigue crack propagation behavior of commercially pure titanium (CP-Ti) using thin-walled cruciform specimens under in-plane biaxial loading, considering the effects of biaxial ratio and phase angle. Increasing phase angle results in more secondary cracks merging with main cracks perpendicular to the rolling direction (RD) and transverse direction (TD), a phenomenon attributed to the rise in shear stress that accelerates main crack growth. Higher loading biaxiality or a lower phase angle leads to decreased crack propagation rates and increased biaxial fatigue life. Electron backscatter diffraction (EBSD) analysis reveals that when the maximum normal stress aligns with the RD, prismatic slip primarily governs crack propagation, thereby accelerating crack propagation rates. Conversely, alignment with the TD reduces prismatic slip activity and crack propagation rates. Under equi-biaxial loading, prismatic slip activity decreases further, and crack propagation is dominated by multiple slip and twinning, consequently resulting in the slowest propagation rates. Additionally, a higher proportion of prismatic slip under high phase angle also accelerates crack propagation. Finally, incorporating Findley equivalent stress into the Chapetti model, which considers the crack length-dependent threshold effect, a highly accurate biaxial small fatigue crack propagation rate model is proposed.

Development of bait station to complement attract-and-kill agents of Zeugodacus tau (Diptera: Tephritidae).

Zeugodacus tau (Walker) (Diptera: Tephritidae) is an important agricultural pest currently managed primarily through the application of insecticides due to limited control strategies. Bait station devices are target specific and have emerged as a behaviorally based alternative to traditional insecticide sprays for managing Z. tau. In this study, we designed a bait station by integrating female-biased olfactory, visual, and gustatory elements, and a killing agent in a wax-matrix. Our results showed that the wax-matrix integrated with spinetoram showed the highest toxicity to immature and mature Z. tau females. Furthermore, the color and shape of the spinetoram bait station significantly influenced its attractiveness and toxicity to female Z. tau. Green sausage-shaped exteriors were the most effective color and shape examined. Subsequent experiments showed a length-dependent effect on mortality and visiting frequencies of Z. tau females when the bait stations were 9-13 cm long. The addition of the olfactory stimulus of 5% ammonium acetate to the bait station attracted a higher number of mature Z. tau females than the control. The killing efficacy of the weathered bait station was similar to that of the fresh station within an 8-week period (over 794 mm of rainfall). The bait station developed herein would provide new insight into the attract-and-kill strategy for Z. tau and alleviate the pressure of the actual management program for this pest.

Controllable One-Step Assembly of Uniform Liquid Crystalline Block Copolymer Cylindrical Micelles by a Tailored Nucleation-Growth Process and Their Application as Tougheners.

The fabrication of uniform cylindrical nanoobjects from soft materials has attracted tremendous research attention from both fundamental research and practical application points of view but has also posed outstanding challenges in terms of their preparation. Herein, we report a one-step method to assemble cylindrical micelles (CMs) with highly controllable lengths from a single liquid crystalline block copolymer by an in situ nucleation-growth strategy. By adjusting the assembly conditions, the lengths of the CMs are controlled from hundreds of nanometers to micrometers. Several influencing factors are systematically investigated to comprehensively understand the process. Particularly, the solvent quality is found determinative in either enhancing or suppressing the nucleation process to produce shorter and longer CMs, respectively. Taking advantage of this strategy, the lengths of CMs can be nicely controlled over a wide concentration range of four orders of magnitude. Lastly, CMs are produced on decent scales and applied as additives to dramatically toughen glassy plastic matrix, revealing an unprecedented length-dependent toughening effect.

Length-Dependent Distinct Cytotoxic Effect of Amyloid Fibrils beyond Optical Diffraction Limit Revealed by Nanoscopic Imaging.

Fibrillar species have been proposed to play an essential role in the cytotoxicity of amyloid peptide and the pathogenesis of neurodegenerative diseases. Discrimination of Aβ aggregates in situ at high spatial resolution is therefore significant for the development of a therapeutic method. In this work, we adopt a rhodamine-like structure as luminescent centers to fabricate carbonized fluorescent nanoparticles (i.e., carbon dots, RhoCDs) with tunable emission wavelengths from green to red and burst-like photoblinking property for localization-based nanoscopic imaging. These RhoCDs contain lipophilic cationic and carboxyl groups which can specifically bind with Aβ1-40 aggregates via electrostatic interaction and hydrogen bonding. According to the nanoscopic imaging in the Aβ1-40 fibrillation and disaggregation process, different types of Aβ1-40 aggregates beyond the optical diffraction limit have been disclosed. Additionally, length-dependent toxic effect of Aβ1-40 aggregates beyond the optical diffraction limit is unveiled. Short amyloid assemblies with length of 187 ± 3.9 nm in the early stage are more toxic than the elongated amyloid fibrils. Second, disassembly of long fibrils into short species by Gramicidin S (GS-2) peptide might enhance the cytotoxicity. These results lay the foundation to develop functional fluorophore for nanoscopic imaging and also provide deep insight into morphology-dependent cytotoxicity from amyloid peptides.

Telomerase regulation by the Pif1 helicase: a length-dependent effect?

Dysfunctional telomere length regulation is detrimental to human health, and both activation and inhibition of telomerase have been proposed in potential therapies to treat human diseases. The Saccharomyces cerevisiae Pif1 protein is an evolutionarily conserved helicase that inhibits telomerase activity at DNA ends. Recent studies have indicated that Pif1 is specifically important for inhibiting telomerase at DNA ends with very little or no telomeric sequence and at long telomeres. At the former, Pif1 prevents the inappropriate addition of a telomere at DNA double-strand breaks. For the latter, Pif1 has been shown to bind long telomeres to presumably promote the extension of the short ones. These observations leave the impression that Pif1 does not act at DNA ends with telomeric sequence of intermediate length. Here, we provide in vivo evidence that Pif1 inhibits telomerase activity at DNA ends regardless of telomere sequence length.

Inulin-Type Fructans Modulates Pancreatic-Gut Innate Immune Responses and Gut Barrier Integrity during Experimental Acute Pancreatitis in a Chain Length-Dependent Manner.

Acute pancreatitis (AP) is a common abdominal inflammatory disorder and one of the leading causes of hospital admission for gastrointestinal disorders. No specific pharmacological or nutritional therapy is available but highly needed. Inulin-type fructans (ITFs) are capable of modifying gut immune and barrier homeostasis in a chemistry-dependent manner and hence potentially applicable for managing AP, but their efficacy in AP has not been demonstrated yet. The current study aimed to examine and compare modulatory effects of ITFs with different degrees of fermentability on pancreatic–gut immunity and barrier function during experimentally induced AP in mice. BALB/c mice were fed short (I)- or long (IV)-chain ITFs supplemented diets for up to 3 days before AP induction by caerulein. Attenuating effects on AP development were stronger with ITF IV than with ITF I. We found that long-chain ITF IV attenuated the severity of AP, as evidenced by reduced serum amylase levels, lipase levels, pancreatic myeloperoxidase activity, pancreatic edema, and histological examination demonstrating reduced pancreatic damage. Short-chain ITF I demonstrated only partial protective effects. Both ITF IV and ITF I modulated AP-associated systemic cytokine levels. ITF IV but not ITF I restored AP-associated intestinal barrier dysfunction by upregulating colonic tight junction modulatory proteins, antimicrobial peptides, and improved general colonic histology. Additionally, differential modulatory effects of ITF IV and ITF I were observed on pancreatic and gut immunity: ITF IV supplementation prevented innate immune cell infiltration in the pancreas and colon and tissue cytokine production. Similar effects were only observed in the gut with ITF I and not in the pancreas. Lastly, ITF IV but not ITF I downregulated AP-triggered upregulation of IL-1 receptor-associated kinase 4 (IRAK-4) and phosphor-c-Jun N-terminal kinase (p-JNK), and a net decrease of phosphor-nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65 (p-NF-κB p65) nuclear translocation and activation in the pancreas. Our findings demonstrate a clear chain length-dependent effect of inulin on AP. The attenuating effects are caused by modulating effects of long-chain inulin on the pancreatic–gut immunity via the pancreatic IRAK-4/p-JNK/p-NF-κBp65 signaling pathway and on prevention of disruption of the gut barrier.

Length-dependent Seebeck effect in single-molecule junctions beyond linear response regime

In the present work, we theoretically study characteristics of the nonlinear Seebeck effect in a single-molecule junction with chain-like bridge of an arbitrary length. We have employed tight-binding models to compute the electron transmission through the system. We concentrate on the analysis of dependences of thermovoltage Vth and differential thermopower S on the bridge length. It is shown that Vth becomes stronger and S grows as the bridge lengthens. We discuss the effects of the bridge coupling to the electrodes and of specific characteristics of terminal sites on the bridge on the length-dependent Vth and S, which appear when the system operates beyond the linear response regime.

Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size.

C9orf72 expansions are the most common genetic cause of FTLD and MND identified to date. Although being intronic, the expansion is translated into five different dipeptide repeat proteins (DPRs) that accumulate within patients’ neurons. Attempts have been made to model DPRs in cell and animals. However, the majority of these use DPRs repeat numbers much shorter than those observed in patients. To address this we have generated a selection of DPR expression constructs with repeat numbers in excess of 1000 repeats, matching what is seen in patients. Small and larger DPRs produce inclusions with similar morphology but different cellular effects. We demonstrate a length dependent effect using electrophysiology with a phenotype only occurring with the longest DPRs. These data highlight the importance of using physiologically relevant repeat numbers when modelling DPRs.

Sarcomere Length Dependent Effects on Ca2+-Induced Troponin Regulation within Chemically Skinned Cardiac Muscle Fibers

Sarcomere length dependent activation (LDA) of myocardial force development is the cellular basis underlying the Frank-Starling law of the heart, but it is still elusive how sarcomere detects the SL changes and converts it into altered activation of thin filament. Among the myofilament proteins, troponin and myosin are two key components that are likely involved in the LDA. The two components are functionally linked through Ca2+ activation and cross-bridge feedback. Although the active cross-bridge feedback has been strongly implicated in LDA, there was no evidence linking sarcomere length changes and troponin regulation until our recent study showing that Ca2+-cTnC interaction in cardiac muscle can be modulated by sarcomere length through cross-bridge feedback [Biophysical Journal, 107(3), 682-93 (2014)]. In this study, we continue our efforts to understand the role of the C-domain of cTnI in LDA. Specifically, we used in situ time-resolved FRET measurements to determine how the switch region of cTnI is affected by Ca2+, sarcomere length, and cross-bridge in skinned cardiac muscle fibers. To monitor the Ca2+-induced structural transition of the switch region, skinned myocardial fibers were reconstituted with troponin complex containing FRET donor (AEDANS) modified cTnI(167C)AEDANS and acceptor (DDPM) modified cTnC(89C)DDPM. The measured FRET distance changes show that Ca2+, strong cross-bridges and sarcomere length all influence the structural transition of the switching region of cTnI within myocardial fibers. The results provide a mechanism by which sarcomere length can modulate Ca2+-troponin regulation via strong cross-bridge binding and suggest that the sarcomere length dependent cross-bridge effect plays an important role in the Frank-Starling law of the heart.

Intra-Cavity Chromatic Dispersion Impacts on 10-Gb/s Optical OFDM Transmissions Over 25-km Dual-RSOA-Based Self-Seeded PON Systems

Owing to the lack of strong physical mechanisms capable of elaborately balancing a large number of longitudinal modes created in a self-seeded passive optical network (PON), the resulting coherent-like optical signals are vulnerable to frequency-dependent optical phenomena such as chromatic dispersion (CD). In this paper, based on our recently reported real-time dual-reflective semiconductor optical amplifier (RSOA)based self-seeded adaptive optical orthogonal frequency-division multiplexing (OOFDM) transmitters, detailed experimental explorations are undertaken, for the first time, of the impacts of intra-cavity CD on 10-Gb/s OOFDM transmissions over 25-km self-seeded PON systems. The cavity length-dependent intra-cavity CD effect is identified to be one of the most prominent physical mechanisms determining the practically achievable performance of such PON systems. In comparison with a 10-m-long dual-RSOA self-seeded fiber cavity, the accumulated intra-cavity CD effect associated with a 1-km-long dual-RSOA self-seeded fiber cavity not only considerably broadens the optical spectral width of the self-seeding-created coherent-like optical signal but increases the signal relative intensity noise (RIN) by 4.8 dB (at a 2-GHz signal spectral region) as well. In addition, a further 1.1-dB signal RIN growth is also measured after the 25-km single-mode fiber (SMF) PON system transmissions. As a direct result, the dual-RSOA self-seeded PON with a 1-km-long fiber cavity suffers from a 2.2-dB power penalty degradation for 10 Gb/s over 25-km SMF intensity modulation and direct detection OOFDM transmissions.

A novel life cycle modeling system for Ebola virus shows a genome length-dependent role of VP24 in virus infectivity.

Work with infectious Ebola viruses is restricted to biosafety level 4 (BSL4) laboratories, presenting a significant barrier for studying these viruses. Life cycle modeling systems, including minigenome systems and transcription- and replication-competent virus-like particle (trVLP) systems, allow modeling of the virus life cycle under BSL2 conditions; however, all current systems model only certain aspects of the virus life cycle, rely on plasmid-based viral protein expression, and have been used to model only single infectious cycles. We have developed a novel life cycle modeling system allowing continuous passaging of infectious trVLPs containing a tetracistronic minigenome that encodes a reporter and the viral proteins VP40, VP24, and GP1,2. This system is ideally suited for studying morphogenesis, budding, and entry, in addition to genome replication and transcription. Importantly, the specific infectivity of trVLPs in this system was ∼ 500-fold higher than that in previous systems. Using this system for functional studies of VP24, we showed that, contrary to previous reports, VP24 only very modestly inhibits genome replication and transcription when expressed in a regulated fashion, which we confirmed using infectious Ebola viruses. Interestingly, we also discovered a genome length-dependent effect of VP24 on particle infectivity, which was previously undetected due to the short length of monocistronic minigenomes and which is due at least partially to a previously unknown function of VP24 in RNA packaging. Based on our findings, we propose a model for the function of VP24 that reconciles all currently available data regarding the role of VP24 in nucleocapsid assembly as well as genome replication and transcription. Ebola viruses cause severe hemorrhagic fevers in humans, with no countermeasures currently being available, and must be studied in maximum-containment laboratories. Only a few of these laboratories exist worldwide, limiting our ability to study Ebola viruses and develop countermeasures. Here we report the development of a novel reverse genetics-based system that allows the study of Ebola viruses without maximum-containment laboratories. We used this system to investigate the Ebola virus protein VP24, showing that, contrary to previous reports, it only modestly inhibits virus genome replication and transcription but is important for packaging of genomes into virus particles, which constitutes a previously unknown function of VP24 and a potential antiviral target. We further propose a comprehensive model for the function of VP24 in nucleocapsid assembly. Importantly, on the basis of this approach, it should easily be possible to develop similar experimental systems for other viruses that are currently restricted to maximum-containment laboratories.

Unusual chain length dependent adsorption of linear and branched alkanes on UiO-66

In this work, the zero coverage adsorption properties of C5–C10 n- and iso-alkanes on the UiO-66, UiO-66-Me and UiO-66-NO2 metal–organic frameworks are studied by gas phase pulse chromatography. Analysis of enthalpy values, entropy values, Gibbs free energies and Henry constants reveals unusual chain length dependent adsorption behaviour of linear and branched alkanes, caused by the complex structure of the zirconium metal–organic framework UiO-66. The UiO-66 structure consists of a small, tetrahedral and large, octahedral cage. It is shown that at specific carbon chain lengths (e.g. C6–C7 for n-alkanes), distinctive jumps in adsorption enthalpy, entropy values and Henry constants occur. This chain length dependent effect is even more pronounced for 2- and 3-methyl alkanes and double branched alkanes. This distinctive shift in adsorption behaviour occurs at a molecular size that coincides with the cavity dimensions of the smallest, tetrahedral cage. The resulting selective adsorption arises from confinement effects and is function of both the molecular shape and size.

Topoisomerases facilitate transcription of long genes linked to autism

Topoisomerases are expressed throughout the developing and adult brain and are mutated in some individuals with autism spectrum disorder (ASD). However, how topoisomerases are mechanistically connected to ASD is unknown. Here we found that topotecan, a Topoisomerase 1 (TOP1) inhibitor, dose-dependently reduced the expression of extremely long genes in mouse and human neurons, including nearly all genes >200 kb. Expression of long genes was also reduced following knockdown of Top1 or Top2b in neurons, highlighting that each enzyme was required for full expression of long genes. By mapping RNA polymerase II density genome-wide in neurons, we found that this length-dependent effect on gene expression was due to impaired transcription elongation. Interestingly, many high confidence ASD candidate genes are exceptionally long and were reduced in expression following TOP1 inhibition. Our findings suggest that chemicals and genetic mutations that impair topoisomerases could commonly contribute to ASD and other neurodevelopmental disorders.

Transient analysis of diffusive chemical reactive species for couple stress fluid flow over vertical cylinder

The unsteady natural convective couple stress fluid flow over a semi-infinite vertical cylinder is analyzed for the homogeneous first-order chemical reaction effect. The couple stress fluid flow model introduces the length dependent effect based on the material constant and dynamic viscosity. Also, it introduces the biharmonic operator in the Navier-Stokes equations, which is absent in the case of Newtonian fluids. The solution to the time-dependent non-linear and coupled governing equations is carried out with an unconditionally stable Crank-Nicolson type of numerical schemes. Numerical results for the transient flow variables, the average wall shear stress, the Nusselt number, and the Sherwood number are shown graphically for both generative and destructive reactions. The time to reach the temporal maximum increases as the reaction constant K increases. The average values of the wall shear stress and the heat transfer rate decrease as K increases, while increase with the increase in the Sherwood number.

Interventricular comparison of the energetics of contraction of trabeculae carneae isolated from the rat heart

We compare the energetics of right ventricular and left ventricular trabeculae carneae isolated from rat hearts. Using our work-loop calorimeter, we subjected trabeculae to stress-length work (W), designed to mimic the pressure-volume work of the heart. Simultaneous measurement of heat production (Q) allowed calculation of the accompanying change of enthalpy (H = W + Q). From the mechanical measurements (i.e. stress and change of length), we calculated work, shortening velocity and power. In combination with heat measurements, we calculated activation heat (Q(A)), crossbridge heat (Q(xb)) and two measures of cardiac efficiency: 'mechanical efficiency' ((mech) = W/H) and 'crossbridge efficiency' ((xb) = W/(H - Q(A))). With respect to their left ventricular counterparts, right venticular trabeculae have higher peak shortening velocity, and higher peak mechanical efficiency, but with no difference of stress development, twitch duration, work performance, shortening power or crossbridge efficiency. That is, the 35% greater maximum mechanical efficiency of right venticular than left ventricular trabeculae (13.6 vs. 10.2%) is offset by the greater metabolic cost of activation (Q(A)) in the latter. When corrected for this difference, crossbridge efficiency does not differ between the ventricles.

Androgen receptor CAG repeat length modifies the effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin on receptor activity in human prostate cells

Increased incidence of prostate cancer has been reported in men exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). TCDD acts through the aryl hydrocarbon receptor (AhR), which interacts with the androgen receptor (AR). The AR gene contains a polymorphic CAG repeat that influences its transcriptional activity. We investigated the influence of TCDD on prostate cancer cells (PC-3) and non-tumor prostate cells (PNT1A) on 5α-dihydrotestosterone-activated ARs containing CAG repeats within normal length range (16, 22, and 28). The AhR target gene CYP1A1 mRNA expression was induced by TCDD, but was not affected by the AR CAG length. TCDD had no effect on AR activity in PC-3 cells, whereas the shortest AR variant was induced by TCDD in PNT1A cells. In conclusion, the CAG length dependent effect of TCDD on AR activity in PNT1A, but not in PC-3 cells, indicates as a cell-specific effect of TCDD on AR activity.

Length-dependent effect of single-walled carbon nanotube exposure in a dynamic cell growth environment of human alveolar epithelial cells

Despite the great use of nanomaterials for engineering and medical applications, nanomaterials may have adverse consequences by accidental exposure, because of their nanoscale size, composition and shape. Like many nanomaterials, carbon nanotubes (CNTs) have been used for many proven applications, but the size of the CNTs makes them more readily become airborne and can therefore create the risk of being inhaled by a worker. In this study, we evaluated single-walled CNT (SWCNT)-induced effects on cellular responses such as cell proliferation, inflammatory response and oxidative stress in dynamic cell growth condition. A dynamic cell growth environment was established to mimic the dynamic changes in the amount of circumferential and longitudinal expansion and contraction occurred during normal breathing movement in the lung. Two different length (short: outer diameter (OD) 1-2 nm, length 0.5-2 μm; long: OD 1-2 nm, length 5-30 μm) of SWCNTs were used at different exposure concentrations (5, 10 and 20 μg/ml) during the different exposure duration (24, 48 and 72 h). Dynamic environment facilitated altered interaction between SWCNTs and A549 monolayer. Cellular responses in dynamic condition were significantly different from those in static condition. Moreover, cellular responses were dependent on the length of SWCNTs both in static and dynamic cell growth conditions.

Methoprene and Temperature Effects on Caste Differentiation and Protein Composition in the Formosan Subterranean Termite,Coptotermes formosanus

The utilization of multiple castes is a shared feature of social insects. In termites, multiple extrinsic factors have been shown to impact caste differentiation; for example, increased temperature has been shown to increase soldier production. Also, application of exogenous methoprene has also been demonstrated to increase soldier production. The objective of this investigation was to examine and correlate the effects of temperature variation and methoprene treatments on termite caste differentiation, and identify the resulting changes in protein levels. Our results indicate that worker—to—soldier differentiation is modulated by temperature, where a greater number of soldiers developed at a higher rate at higher temperatures compared to lower temperatures. We analyzed total protein by sodium dodecyl sulfate Polyacrylamide gel electrophoresis and N-terminal sequencing and found several changes. Specifically, four proteins affected by temperature change were identified: Hexamerin-1, Hexamerin-2, Endo-beta 1,4 glucanase, and myosin. These proteins were further examined for their response to temperature, assay length (time), and exposure to the juvenile hormone analog methoprene. Hexamerin-1 protein showed a temperature—and assay length—dependent effect, while Hexamerin-2, Endo-beta 1, 4 glucanase, and myosin protein levels were all affected by temperature, assay length, and exposure to methoprene. Our analysis allows the correlation of temperature, assay length, and presence of methoprene with specific changes in protein levels that occur during caste differentiation. These results can be directly applied to better understand the complex developmental factors that control termite differentiation and guide the use of juvenile hormone analogs to maximize efficiency of termite eradication in the field.

Persistent organic pollutants have dose and CAG repeat length dependent effects on androgen receptor activity in vitro

Recently, the effect of exposure to persistent organic pollutants (POPs) on sperm concentration was only seen in men with a short androgen receptor (AR) gene CAG repeat. In order to investigate whether these effects could be observed also in vitro, we tested the impact of 2,2′,4,4′,5,5′-hexachlorobiphenyl (CB-153) and 1,1- bis-(4-chlorophenyl)-2,2-dichloroethene (4,4′-DDE) on 5α-dihydrotestosterone activated ARs containing 16, 22 and 28 CAG repeats, respectively. Single exposure to 4,4′-DDE had the most pronounced effect on the AR activity containing 16 CAG repeats, whereas 28 CAG was the most sensitive variant when a mixture of the two compounds was added. Thus, our in vitro results have confirmed the in vivo data indicating a CAG repeat length dependent effect of endocrine disrupters on the AR activity.

Numerical Analysis of Couple Stress Fluid Past an Infinite Vertical Cylinder

:Numerical analysis is performed to study the transient free convective boundary layer flow of a couple stress fluid past an infinite vertical cylinder, in the absence of body forces and body couples. A set of nondimensional governing equations, namely, the continuity, momentum and energy equations are derived, which are unsteady, non-linear and coupled. The couple stress fluid flow model introduces the length dependent effect based on the material constant and dynamic viscosity. Also, it introduces the biharmonic operator in the Navier-Stokes equations, which is not present in the case of Newtonian fluids. As there are no analytical or direct numerical method available to solve these unsteady, non-linear and coupled equations, they are solved using the CFD techniques. An unconditionally stable Crank-Nicolson type of implicit finite difference scheme is employed to obtain the discretized forms of the governing equations. The discretized equations are solved using the Thomas and pentadiagonal algorithm. The results concerning the velocity and temperature profiles across the boundary layer are illustrated graphically and discussed for different values of Prandtl number. Transient effects of velocity and temperature are analyzed and compared with those of the Newtonian fluids. The heat transfer characteristics are analyzed and compared with those of Newtonian fluids with the help of average skin-friction and Nusselt number and are shown graphically. It is observed that the deviation of transient velocity and temperature profiles from the hot wall of a couple stress fluid is more in comparison with that of Newtonian fluids.