Leguminous Species Research Articles

Contrasted agronomical and physiological responses of five Coffea arabica genotypes under soil water deficit in field conditions.

Breeding programs have developed high-yielding Coffea arabica F1-hybrids as an adaptation against adverse conditions associated with climate change. However, theresponse to drought of coffee F1 hybrids has seldom been assessed. A trial was established with five C. arabica genotypes (2 pure lines: Catimor and Marsellesa and 3 F1 hybrids: Starmaya, Centroamericano and Mundo Maya) planted under the leguminous tree species Leuceana leucocephala. Coffee growth, yield and physiological responses were assessed under a rain-fed (control: CON) and a rainfall reduction treatment (RR) for 2 years. The RR treatment created a long-term rainfall deficit in a region with suboptimal temperature similar to those predicted by climate change scenarios. Moreover, the RR treatment reduced soil water content by 14% over 2 successive years of production and increased hydric stress of the three F1-hybrids (leaf water potentials averaged -0.8 MPa under RR compared with -0.4 MPa under CON). Under RR, coffee yields were reduced from 16 to 75% compared to CON. Mundo Maya F1 hybrid was the sole high-yielding genotype apable of sustaining its yield under RR conditions. Our results suggested that its significant increase in fine root density (CON = 300 and RR = 910 root.m-2) and its maintenance of photosynthetic rate (2.5 - 3.5 mmol CO2 m-2 s-1) at high evaporative demand might explain why this genotype maintained high yield under RR condition. This work highlights a possible drought tolerance mechanism in fruit bearing adult coffee trees where the plant fine root number increases to intake more water in order to preserve turgor and sustainphotosynthesis at high ETo and therefore conserves high yield in dry conditions.

Little owl (Athene noctua) pellets enhance seed germination under salinity stress in two leguminous plant species

ABSTRACT As central-place foragers, owls accumulate prey remains, in the form of rejection pellets, around their roosting and nesting sites, which may modify top soil composition and influence seed germination. However, the effects of owl pellets on seed germination still remain understudied. In this study, we conducted germination assays on two plant species from south Tunisia to assess whether a prior treatment of the seeds with the filtrate of little owl (Athene noctua) pellets affected germination rates under different salinity conditions. Results showed enhanced germination in pellet-treated seeds compared to untreated seeds. Pellet treatment was also associated with reduced inhibitory effects of salt on seed germination. Moreover, at the scale of a little owl territory, the concentrations of organic matter and total nitrogen in the top soil were higher at the nesting site compared to a nearby unused site, providing some evidence of a fertilizing effect of pellets. Overall, our findings suggest that little owls play a significant role in shaping plant dynamics and ecosystem functioning in arid North African areas, where the soil is poor and threatened by salinization. We suggest that little owls may be relevant actors in eventual restoration and rehabilitation plans of degraded ecosystems in these areas.

Sulfur availability and nodulation modify the response of Robinia pseudoacacia L. to lead (Pb) exposure

Both sulfur (S) supply and legume-rhizobium symbiosis can significantly contribute to enhancing the efficiency of phytoremediation of heavy metals (HMs). However, the regulatory mechanism determining the performance of legumes at lead (Pb) exposure have not been elucidated. Here, we cultivated black locust (Robinia pseudoacacia L.), a leguminous woody pioneer species at three S supply levels (i.e., deficient, moderate, and high S) with rhizobia inoculation and investigated the interaction of these treatments upon Pb exposure. Our results revealed that the root system of Robinia has a strong Pb accumulation and anti-oxidative capacity that protect the leaves from Pb toxicity. Compared with moderate S supply, high S supply significantly increased Pb accumulation in roots by promoting the synthesis of reduced S compounds (i.e., thiols, phytochelatin), and also strengthened the antioxidant system in leaves. Weakened defense at deficient S supply was indicated by enhanced oxidative damage. Rhizobia inoculation alleviated the oxidative damage of its Robinia host by immobilizing Pb to reduce its absorption by root cells. Together with enhanced Pb chelation in leaves, these mechanisms strengthen Pb detoxification in the Robinia-rhizobia symbiosis. Our results indicate that appropriate S supply can improve the defense of legume-rhizobia symbiosis against HM toxicity.

Sophoridine exerts anti-arthritic effects on fibroblast-like synoviocytes and collagen-induced arthritis in rats.

The discovery of alternative medicines with fewer adverse effects is urgently needed for rheumatoid arthritis (RA). Sophoridine (SR), the naturally occurring quinolizidine alkaloid isolated from the leguminous sophora species, has been demonstrated to possess a wide range of pharmacological activities. However, the effect of SR on RA remains unknown. In this study, the collagen-induced arthritis (CIA) rat model and tumor necrosis factor alpha (TNFα)-induced fibroblast-like synoviocytes (FLSs) were utilized to investigate the inhibitory effect of SR on RA. The anti-arthritic effect of SR was evaluated using the CIA rat model in vivo and TNFα-stimulated FLSs in vitro. Mechanistically, potential therapeutic targets and pathways of SR in RA were analyzed through drug target databases and disease databases, and validation was carried out through immunofluorescence, immunohistochemistry, and Western blot. The in vivo results revealed that SR treatment effectively ameliorated synovial inflammation and bone erosion in rats with CIA. The in vitro studies showed that SR could significantly suppress the proliferation and migration in TNFα-induced arthritic FLSs. Mechanistically, SR treatment efficiently inhibited the activation of MAPKs (JNK and p38) and NF-κB pathways in TNFα-induced arthritic FLSs. These findings were further substantiated by Immunohistochemistry results in the CIA rat. SR exerts an anti-arthritic effect in CIA rats through inhibition of the pathogenic characteristic of arthritic FLSs via suppressing NF-κB and MAPKs (JNK and p38) signaling pathways. SR may have a great potential for development as a novel therapeutic agent for RA treatment.

Tuber Development and Tuber Yield Potential of Winged Bean (Psophocarpus tetragonolobus (L.) DC.), an Alternative Crop for Animal Feed

The winged bean is only one of the members of the leguminous species that has the potential for duo-purpose for its pod and tuber yields. The protein and nutritional content in the tuber can be used as alternative crops for animal feed. Previous studies identified the winged bean varieties as having high potential for tuber production, although their tuber development remains unclear. This study aims to elucidate the development of winged bean tubers based on their size and weight during eight months of growth. Tuber yields of high-potential winged bean varieties (W018 and W099) were compared to commercial varieties (Ratchaburi) under field conditions over two years. The experiments were laid out in randomized complete block design with three replications in the 2021–2022 and 2022–2023 growing seasons. The tuber formation of winged bean exhibited a significant increase in tuber weight and size in the fourth month of growth in all varieties, with a gradual change from fibrous roots to tubers. Particularly in the W018 and W099 varieties, tuber development persisted up to eight months after transplanting. On the other hand, the Ratchaburi variety had small tuber size, resulting in the tubers weighing less than in the W018 and W099 varieties. The results indicated that winged bean varieties had different tuber development, with the W018 and W099 varieties being suitable for tuber harvesting at eight months. The large tuber size can be an appropriate alternative to animal feed materials. At the same time, the Ratchaburi variety requires only five months since it is cultivated primarily for tuber consumption, which is concerned with size and shape. Moreover, the yields of the W018 and W099 varieties were significantly higher compared to Ratchaburi at 22.4, 19.3, and 7.423 T/ha in the 2021–2022 growing season and 8.4, 7.9, and 5.6 T/ha in the growing season of 2022–2023, respectively. The protein content in the winged bean tubers increased to 13.00% at eight months. The years (Y) and genotypes (G) differed considerably for tuber formation. The variation in both Y and G and the shoot/root ratio are also discussed in this study.

Divergent responses of above‐ and belowground ecosystem functioning to shrub encroachment in the Tibetan semi‐arid alpine steppes

AbstractSemi‐arid alpine ecosystems on the Tibetan Plateau are experiencing rapid climate change and extensive anthropogenic activity, concomitant with the encroachment of shrubs. Shrub encroachment will lead to changes in both the ecosystem structure and functioning of the semi‐arid alpine steppes. Yet, the encroaching impacts of shrubs on the above‐ and belowground ecosystem functioning of the semi‐arid alpine steppes remain uncertain. To quantify shrub encroachment impacts on ecosystem functioning of the semi‐arid alpine steppes, two alpine steppe sites encroached by shrub species are investigated, with one site encroached by leguminous shrub species (Caragana spinifera) and another site encroached by non‐leguminous shrub species (Dasiphora fruticosa). Results shows that the individual ecosystem functions and the ecosystem multifunctionality (EMF) of alpine steppes are significantly enhanced following both leguminous and non‐leguminous shrubs encroachment. We conclude that shrub encroachment tends to facilitate the belowground EMF (BEMF) but has neutral effects on the aboveground EMF (AEMF) in alpine steppes. Our findings also highlight that soil nutrients play critical roles in driving ecosystem functioning responses to shrubs encroachment. These findings further our understanding of shrub encroachment impacts on the ecosystem functioning of the semi‐arid alpine steppes.

Phytochemical Compounds and Antioxidant Activity of Two Extracts of Wild and Domesticated Carob Leaves.

<b>Background and Objective:</b> Carob tree (<i>Ceratonia siliqua</i> L.) is a perennial leguminous species and is known as a medicinal importance tree. This species exhibits a myriad of biological effects including antibacterial, antidiarrheal and antidiabetic. To this end, current study evaluates the difference between the phytochemical composition of the leaves of two accessions of "wild" and "domesticated" hermaphroditic carob trees. <b>Materials and Methods:</b> The comparison between two carob accessions "wild" and "domesticated" was done according to methanolic extraction by the Soxhlet and aqueous extraction by maceration. The polyphenols, flavonoids, tannins and their antioxidant activity were measured. The ANOVA test was used for the analysis of results. <b>Results:</b> The total polyphenols in aqueous extract are 6.19±0.25 mg equivalent gallic acid/g dry weight (EGA/g DW) and 4.23±0.2 mg EGA/g DW) in carob fresh leaves for wild and domesticated trees, respectively. The flavonoid content was higher in methanolic extract (3.17±0.64 mg quercetin equivalent/g DW) than in aqueous extract (1.06±0.19 mg EQ/g DW) for wild trees. Wild accession recorded the highest concentration of condensed tannins in the methanolic extract (6.4±0.3 mg catechin equivalents/g DW) while low levels were recorded in aqueous extract (0.51±0.27 mg EC/g DW). <b>Conclusion:</b> Such knowledge is expected to be the key to understanding the biochemical composition of two different leaves of <i>C. siliqua</i> accessions and its various commercial food products.

Evaluation of minerals, trace elements, and antinutritional factors in selected legume fodder species (Fabaceae) with the potential to improve cattle nutrition and gastrointestinal health

The study aimed to investigate the nutritional composition, trace elements and anti-nutritional factors of fodder species belonging to the family Fabaceae potentially used as an alternative feed for cattle. The proximate composition, particularly dry matter, moisture, fats, crude proteins (CP), carbohydrates, crude fibre (CF), and neutral detergent fiber (NDF), were analysed, thereby, nonfibre carbohydrate (NFC) and gross energy (GE) were calculated. Thirty-three trace elements were determined from chemically digested dried plant material using ICP-MS (Inductively Coupled Plasma Mass Spectrometry) and ICP-OES (ICP-Optical Emission Spectrophotometry). The tannin levels, a known antinutritional factor, were estimated using Folin–Ciocalteu method. The methods were validated by the relative standard deviation (RSD) values and acceptable recovery percentage, linearity, limit of quantification (LoQ), and limit of detection (LoD). The proximate composition analysis estimated levels of dry matter (> 90 %), ash (3.77–26.98 %), crude proteins (8.22–22.19 %), carbohydrates (54.00–86.79 %), crude fibre (10.54–40.10 %), NDF (22.26–59.20 %) and GE (< 100 Kcal kg−1 DM) in leguminous species. Essential elements were detected in recommended levels including Zn (21.20–50.30 mg/kg), Co (0.06–0.045 mg kg), Cr (0.5–5.08 mg kg−1), Mn (9.02–197 mg kg−1), Mg (0.10–0.52 mg kg−1), Fe (42.40–812 mg kg−1) and Na (72.00–1721 mg kg−1). The concentration of toxic elements was below critical levels and tannin occurred at a safe level (< 50 mgTAE kg−1) for ruminant consumption. Therefore, the selected fodder can effectively contribute to cattle dietary requirements for smallscale farmers in Onderstepoort, Gauteng Province, South Africa.

Elevated CO2 concentration enhance carbon and nitrogen metabolism and biomass accumulation of Ormosiahosiei

Elevated CO2 concentrations may inhibit photosynthesis due to nitrogen deficiency, but legumes may be able to overcome this limitation and continue to grow. Our study confirms this conjecture well. First, we placed the two-year-old potted saplings of Ormosia hosiei (O. hosiei) (a leguminous tree species) in the open-top chamber (OTC) with three CO2 concentrations of 400 (CK), 600 (E1), and 800 μmol·mol−1 (E2) to simulate the elevated CO2 concentration environment. After 146 days, the light saturation point (LSP), light compensation point (LCP), apparent quantum efficiency (AQE), and dark respiration rate (Rd) of O. hosiei were increased under increasing CO2 concentration and obtain the maximum ribulose diphosphate (RuBP) carboxylation rate (Vc max) and RuBP regenerated photosynthetic electron transfer rate (Jmax) were also significantly increased under E2 treatment (P < 0.05). This results in a significant increase of the maximum assimilation rate (Amax) under elevated CO2 concentrations. Sucrose phosphate synthase (SPS) activity in sucrose metabolism increased in the leaves, more soluble sugars, starches, and sucrose was produced, but sucrose content only in leaves increased at E2, and more carbon flows to the roots. The activity of the NH4+ assimilating enzymes glutamine synthetase (GS), glutamate synthetase (GOGAT), and glutamate dehydrogenase (GDH) in the leaves of O. hosiei increases under elevated CO2 concentrations to promote nitrogen synthesis that reduces the content of ammonium nitrogen and increases the content of nitrate nitrogen. In addition, under E1 conditions, sucrose synthase (SS), direction of synthesis activity was highest and sucrose invertase (INV) activity was lowest, this means that the balance of C and N metabolism is maintained. While under E2 conditions SS activity decreased and INV activity increased, this increased C/N and nitrogen use efficiency. So, the elevated CO2 concentration promotes the accumulation of O. hosiei biomass, especially in the aboveground part, but did not have a significant effect on the accumulation of root biomass. This means that O. hosiei is able to cope under the elevated CO2 concentration without showing photosynthetic adaptation during the experimental period.

Responses of soil enzymatic activities and microbial biomass phosphorus to improve nutrient accumulation abilities in leguminous species

Fertilizers application are widely used to get a higher yield in agricultural fields. Nutrient management can be improved by cultivating leguminous species in order to obtain a better understanding of the mechanisms that increase the amount of available phosphorus (P) and potassium (K) through fertilizer treatments. A pot experiment was conducted to identify the leguminous species (i.e., chickpea and pea) under various fertilizer treatments. Experimental design is as follows: T0 (control: no fertilizer was applied), T1: P applied at the level of (90 kg ha−1), T2: (K applied at the level of 90 kg ha−1), and T3: (PK applied both at 90 kg ha−1). All fertilizer treatments significantly (p < 0.05) improved the nutrient accumulation abilities and enzymes activities. The T3 treatment showed highest N uptake in chickpea was 37.0%, compared to T0. While T3 developed greater N uptake in pea by 151.4% than the control. However, T3 treatment also increased microbial biomass phosphorus in both species i.e., 95.7% and 81.5% in chickpeas and peas, respectively, compared to T0 treatment. In chickpeas, T1 treatment stimulated NAGase activities by 52.4%, and T2 developed URase activities by 50.1% higher than control. In contrast, T3 treatment enhanced both BGase and Phase enzyme activities, i.e., 55.8% and 33.9%, respectively, compared to the T0 treatment. Only the T3 treatment improved the activities of enzymes in the pea species (i.e., BGase was 149.7%, URase was 111.9%, Phase was 81.1%, and NAGase was 70.0%) compared to the control. Therefore, adding combined P and K fertilizer applications to the soil can increase the activity of enzymes in both legume species, and changes in microbial biomass P and soil nutrient availability make it easier for plants to uptake the nutrients.

Mitigation of oxidative stress and inflammatory factors, along with the antibrowning and antimicrobial effects of cassia seed microbial fermentation solution.

Cassia seeds, originating from the mature seeds of leguminous cassia species, possess pharmacological effects attributed to their rich composition of various active ingredients, notably anthraquinones. While current research predominantly focuses on pharmaceutical extractions, there has been limited progress in fermentation studies. Our study aimed to enhance the content of active compounds such as anthraquinones, flavonoids, and polyphenols using microbial fermentation techniques. We specifically optimized a fermentation system through a single-factor experimental design. The antioxidant properties of the fermentation solution were validated through assays involving HaCaT cells and zebrafish. We observed effective suppression of inflammatory reactions in both RAW264.7 cells and transgenic zebrafish by the fermentation solution. Moreover, significant inhibition of tyrosinase activity and melanin production was evident in B16-F10 cells and zebrafish. Positive outcomes were also obtained in antibacterial assays and chick embryo experiments. These findings highlight the potential of cassia seed fermentation solution as a safe and eco-friendly material in food chemistry and biomedical sciences.

Influence of Carbons on Metal Stabilization and the Reduction in Soil Phytotoxicity with the Assessment of Health Risks

Despite notable achievements in the development and application of the remediation of metal-contaminated soils, the search for and study of promising immobilizing agents continues. This article presents a new approach to using brown coal and activated carbon and their application for metal stabilization. An experiment was conducted to test Medicago falcata L. on soils from industrial areas contaminated with varying levels of lead (Pb), cadmium (Cd), chromium (Cr), zinc (Zn), copper (Cu), and nickel (Ni) with the addition of carbons. This plant is a stress-tolerant leguminous species. In this study, the total content of metals in soil and the available forms to plants (single extractions with 0.02 M ethylenediaminetetraacetic acid, EDTA) were determined, along with metal contents in the plant. The use of carbons lowered the phytoavailable forms of metals for plants and thus, resulted in a reduction in the phytotoxicity of the soils. The contents of the tested metals in the roots and shoots were lower than in the combinations of soils with no carbon added. The activated carbon had a stronger effect on limiting the availability of metals than brown coal in relation to plants growing on soils without added carbon; the percentage of reduction for the shoots was Cr (18.2%) &gt; Zn (11.5%) &gt; Ni (10.7%) &gt; Cu (10.3%) &gt; Cd (8.9%) &gt; Pb (2.4%) and Cu (13.3%) &gt; Cr (12.5%) &gt; Zn (10.5%) &gt; Pb (9.0%) &gt; Ni (5.7%) &gt; Cd (4.6%) for roots. Metals reduced the growth of Medicago falcata L. roots from 44 to 21%, while the growth of shoots was reduced from 25 to 2%. Adding carbons to soils in all combinations resulted in a decrease in the following pollution indices: pollution index (PI), pollution load index (PLI), and non-carcinogenic (HQ) and carcinogenic risk factors (ILCR).

Nutrient Contribution and Carbon Sequestration of an Agroforestry System of Coffea canephora Cultivated by Conventional and Organic Management in the Ecuadorian Amazon

Agroforestry systems (AFSs) seek synergies that improve productivity, sustainability, and environmental benefits. This is achieved through the supplying of nutrients to the soil, carbon storage, and sequestration. In the Ecuadorian Amazon, Coffea canephora is planted together with leguminous, woody, forest, and secondary forest species, where the continuous incorporation of vegetative residues from shade species represents a substantial addition of nutrients within these systems. This study was carried out from 2018 to 2022 to determine the contribution of nutrients contained in the biomass and C sequestration in agroforestry systems of coffee with conventional (high use of agrochemicals) and organic (without the use of chemicals) management. The study was carried out with a randomized complete block design, using a factorial arrangement (2 × 4 with three replications). This arrangement included two types of systems (agroforestry and monoculture) and four agronomic management practices (high and medium for conventional, and intensive and low organic). The biomass and nutrient content were measured twice a year (every 180 days); in addition, the yield was also recorded. A multivariate and univariate analysis was used for data analysis through R and SAS software. After five years of evaluation, it was determined that the N, K, Ca, and Mg contents were higher in the agroforestry systems than the monocultures. In the AFSs, the highest nutrient content was obtained with the medium conventional and low organic agronomic management, while in the monocultures, it was obtained with the high and medium conventional management. In addition, at a soil depth of 20 cm, the total storage and CO2 were 38.12 and 139.8 t ha−1, respectively. The highest yields were obtained with conventional management in AFSs (1599 kg ha−1) and monoculture (1789.45 kg ha−1). Overall, AFSs showed a significant contribution of nutrients, such as N, K, Ca, and Mg, for coffee cultivation; moreover, yields were similar in the AFS and monoculture with both conventional and organic management, which is positive, since AFSs also contribute environmental benefits.

Ecological aspects and relationships of the emblematic Vachellia spp. exposed to anthropic pressures and parasitism in natural hyper-arid ecosystems: ethnobotanical elements, morphology, and biological nitrogen fixation

Main conclusionEmblematic Vachellia spp. naturally exposed to hyper-arid conditions, intensive grazing, and parasitism maintain a high nitrogen content and functional mutualistic nitrogen-fixing symbioses.AlUla region in Saudi Arabia has a rich history regarding mankind, local wildlife, and fertility islands suitable for leguminous species, such as the emblematic Vachellia spp. desert trees. In this region, we investigated the characteristics of desert legumes in two nature reserves (Sharaan and Madakhil), at one archaeological site (Hegra), and in open public domains at Al. Ward and Jabal Abu Oud. Biological nitrogen fixation (BNF), isotopes, and N and C contents were investigated through multiple lenses, including parasitism, plant tissues, species identification, plant maturity, health status, and plant growth. The average BNF rates of 19 Vachellia gerrardii and 21 Vachellia tortilis trees were respectively 39 and 67%, with low signs of inner N content fluctuations (2.10–2.63% N) compared to other co-occurring plants. The BNF of 23 R. raetam was just as high, with an average of 65% and steady inner N contents of 2.25 ± 0.30%. Regarding parasitism, infected Vachellia trees were unfazed compared to uninfected trees, thereby challenging the commonly accepted detrimental role of parasites. Overall, these results suggest that Vachellia trees and R. raetam shrubs exploit BNF in hyper-arid environments to maintain a high N content when exposed to parasitism and grazing. These findings underline the pivotal role of plant-bacteria mutualistic symbioses in desert environments. All ecological traits and relationships mentioned are further arguments in favor of these legumes serving as keystone species for ecological restoration and agro-silvo-pastoralism in the AlUla region.Graphical abstract

Microbiome properties in the root nodules of Prosopis cineraria, a leguminous desert tree.

We conducted a comprehensive analysis of the total microbiome and transcriptionally active microbiome communities in the roots and root nodules of Prosopis cineraria, an important leguminous tree in arid regions of many Asian countries. Mature P. cineraria trees growing in the desert did not exhibit any detected root nodules. However, we observed root nodules on the roots of P. cineraria growing on a desert farm and on young plants growing in a growth chamber, when inoculated with rhizosphere soil, including with rhizosphere soil from near desert tree roots that had no nodules. Compared to nearby soil, non-nodulated roots were enriched with Actinobacteria (e.g., Actinophytocola sp.), whereas root nodules sampled from the desert farm and growth chamber had abundant Alphaproteobacteria (e.g., Ensifer sp.). These nodules yielded many microbes in addition to such nitrogen-fixing bacteria as Ensifer and Sinorhizobium species. Significant differences exist in the composition and abundance of microbial isolates between the nodule surface and the nodule endosphere. Shotgun metagenome analysis of nodule endospheres revealed that the root nodules comprised over 90% bacterial DNA, whereas metatranscriptome analysis showed that the plant produces vastly more transcripts than the microbes in these nodules. Control inoculations demonstrated that four out of six Rhizobium, Agrobacterium, or Ensifer isolates purified from P. cineraria nodules produced nodules in the roots of P. cineraria seedlings under greenhouse conditions. The best nodulation was achieved when seedlings were inoculated with a mixture of those bacterial strains. Though root nodulation could be achieved under water stress conditions, nodule number and nodule biomass increased with copious water availability. .IMPORTANCEMicrobial communities were investigated in roots and root nodules of Prosopis cineraria, a leguminous tree species in arid Asian regions that is responsible for exceptionally important contributions to soil fertility in these dramatically dry locations. Soil removed from regions near nodule-free roots on these mature plants contained an abundance of bacteria with the genetic ability to generate nodules and fix nitrogen but did not normally nodulate in their native rhizosphere environment, suggesting a very different co-evolved relationship than that observed for herbaceous legumes. The relative over-expression of the low-gene-density plant DNA compared to the bacterial DNA in the nodules was also unexpected, indicating a very powerful induction of host genetic contributions within the nodule. Finally, the water dependence of nodulation in inoculated seedlings suggested a possible link between early seedling growth (before a deep root system can be developed) and the early development of nitrogen-fixing capability.

Molecular identification of DNA barcoding of Leguminous toxic species and quantitative analysis by ELISA kits

Molecular identification of DNA barcoding of Leguminous toxic species and quantitative analysis by ELISA kits

Assessing the Potential of Parkia biglobosa (Jacq.) R.Br. ex. G. Don - A Leguminous Plant Species commonly found in Nigeria to Decontaminate Crude Oil-polluted Soil in Terrestrial Ecosystem

The potentials of Parkia biglobosa (a leguminous plant species) to decontaminated crude oil-polluted soil in terrestrial ecosystem was evaluated in this study using appropriate standard techniques to estimate seedling germination, height, collar diameter, number of leaves and physicochemical characteristics (organic matter, pH, calcium, magnesium and sodium, nitrogen, phosphorous and potassium) of soil used, were determined using standard protocols. Results indicated that varying concentration of crude oil influenced the variables measured. For 0 ml, 25 ml, 50 ml, 75 ml and 100 ml crude oil treatments, Parkia biglobosa had 18%, 18%, 16%, 13% and 11% germination rate correspondingly; mean height of 38.70cm, 26.80cm, 21.20cm, 18.50cm and 6.80cm respectively, mean collar diameter of 0.32mm, 0.30mm, 0.26mm, 0.24mm and 0.22mm respectively; 2, 0, 0, 0 and 0 nodules count respectively, and 8.00, 6.00, 5.00, 5.00 and 2.00 leaf count respectively. All physicochemical parameters measured decreased in the soil in inverse proportion to crude oil concentration. So also, seedling germination, height, collar diameter, number of leaves and nodulation decreased in inverse proportion to crude oil concentration. Parkia biglobosa grew tolerably in both high and low concentrations of crude oil, which is an indication of its able to withstand crude oil toxicity. Parkia biglobosa as a nitrogen fixing plant would be a good candidate for the terrestrial restoration of crude oil contaminated regions in Nigerian.

Ecological niche modelling of Indigofera oblongifolia (Forssk.): a global machine learning assessment using climatic and non-climatic predictors

Climate change and other extinction facilitators have caused significant shifts in the distribution patterns of many species during the past few decades. Restoring and protecting lesser-known species may be more challenging without adequate biogeographical information. To address this knowledge gap, the current study set out to determine the global spatial distribution patterns of Indigofera oblongifolia (Forssk) a relatively lesser-known leguminous species. This was accomplished by utilizing three distinct bioclimatic temporal frames (current, 2050, and 2070) and four greenhouse gas scenarios (RCPs 2.6, 4.5, 6.0, and 8.5), in addition to non-climatic predictors such as global livestock population, human modification of terrestrial ecosystems, and global fertilizers application (nitrogen and phosphorus). Furthermore, we evaluate the degree of indigenousness using the geographical area, habitat suitability categories, and number of polygons. This research reveals that climatic predictors outperform non-climatic predictors in terms of improving model quality. Precipitation Seasonality is one of the most important factors influencing this species' optimum habitat suitability up to 150 mm for the current, 2050 RCP 8.5 and 2070-RCPs 2.6, 4.5, and 8.5. Our ellipsoid niche modelling extends the range of precipitation during the wettest quarter and maximum temperature during the warmest month to 637 mm and 26.5–31.80 degrees Celsius, respectively. India has a higher indigenous score in the optimal class than the African region. This findings suggest that the species in question tends to occupy contiguous regions in Africa, while in India, it is dispersed into several smaller meta-populations.

Pierid Butterflies, Legume Hostplants, and Parasitoids in Urban Areas of Southern Florida.

Are parasitoids less likely to find their Lepidoptera hosts on non-native hostplants than native hostplants? We predicted that with longer periods of coevolution between herbivores and the plants they consume, the parasitoids that provide top-down control would be more attuned to finding their hosts on native plants. To test this hypothesis, we collected immature stages of sulfur butterflies (the cloudless sulfur (Phoebis sennae) and the orange-barred sulfur (Phoebis agarithe) over a three-year period (2008-2011) from native and ornamental hostplants in the genus Senna in three different parts of the urban landscape of Miami, Florida, USA. We reared the immature specimens to pupation and either eclosion of adults or emergence of parasitoids and compared the levels of parasitization among the three areas, and among native vs. exotic hostplants. We found, contrary to our prediction, that caterpillars feeding on non-native leguminous hostplant species were more likely to be parasitized than those feeding on native hostplants. We discuss this surprising finding in the light of recent findings in other plant/herbivore/parasitoid systems.

PRODUCTIVITY OF ELEPHANT GRASS (Pennisetum purpureum) PLANTED WITH LEGUMINOSE AT VARIOUS DOSAGES OF BIOORGANIC FERTILIZER

&#x0D; &#x0D; &#x0D; The purpose of this study was to determine the productivity of elephant grass (Pennisetum purpureum) planted with leguminosae at various doses of bioorganic fertilizers. This study used a completely randomized design with two factorial patterns. The first factor was the type of leguminosae (T= without leguminosae; R= Arachi pintoi; L= Alysicarpus vaginalis; and the second factor was the dose of bioorganic fertilizer (D0= 0 kg Nha-1; D1= 100 kg Nha-1; D2= 200 kg Nha-1; and D3= 300 kg Nha-1) There were 12 treatment combinations, each treatment was repeated 5 times so that it consisted of 60 experimental units. The observed variables included growth, yield and growth characteristics variable. The results showed that there was no interaction between leguminous species and fertilizer dosage, both leguminous species only increased leaf area per pot, bioorganic fertilizer D1 and D2 doses tended to increase, while D3 dose increased leaf dry weight, total forage dry weight, and leaf area per pot of elep- hant grass (Pennisetum purpureum). It was concluded that the type of leguminous did not affect the productivity of elephant grass, the dose of bioorganic fertilizer increased crop yields, the highest yields at a dose of 300 kg Nha-1 bioorganic fertilizer.&#x0D; &#x0D; &#x0D;