Background: Hamstring tightness is a common issue that can hinder athletic performance and increase the risk of injury. Effective interventions are needed to enhance hamstring range of motion (ROM) and muscle performance. This study aims to compare two manual therapy techniques: the Mulligan Bent Leg Raise technique and the PNF Hold-Relax with Agonist Contraction technique. Objective: The primary objective is to compare the immediate effects of these two techniques on hamstring ROM and muscle performance. Secondary objectives include evaluating participants’ perceived comfort during the interventions and exploring the practical application of both techniques in clinical settings. Methods: A review of literature, focusing on randomized controlled trials involving participants aged 18–35 years, was conducted. Participants were divided into two groups: one receiving the Mulligan Bent Leg Raise technique and the other undergoing PNF Hold-Relax with Agonist Contraction. Pre- and post-intervention measurements of hamstring ROM and muscle performance were collected. Immediate effects were recorded and analyzed to compare outcomes between the two techniques. Results: Preliminary results indicate significant immediate improvements in hamstring flexibility and muscle performance for both techniques. Literature supports the effectiveness of both the Mulligan and PNF techniques in enhancing hamstring function. Conclusion: Evidence suggests that both the Mulligan Bent Leg Raise and PNF Hold-Relax with Agonist Contraction techniques effectively improve hamstring flexibility and muscle performance. Implications: This study provides valuable insights for physiotherapy practice, equipping clinicians with evidence-based strategies to address hamstring tightness and dysfunction. By integrating these techniques, physiotherapists can achieve rapid improvements and enhance overall treatment outcomes.