Leccinum Scabrum Research Articles

Hypoglycemic Properties of Leccinum scabrum Extracts-An In Vitro Study on α-Glucosidase and α-Amylase Inhibition and Metabolic Profile Determination.

Type-2 diabetes affects an increasing percentage of the world's population and its control through dietary management, involving the consumption of health-promoting foods or their derived supplements, is a common strategy. Several mushroom species have been demonstrated to be endowed with antidiabetic properties, resulting from their ability in improving insulin sensitivity and production, or inhibiting the carbohydrate-hydrolyzing enzymes α-amylase and α-glucosidase. This study aimed to investigate for the first time the hypoglycemic properties of the edible mushroom Leccinum scabrum (Bull.) Gray. Mushroom extracts were prepared through the microwave-assisted extraction (MAE) technique using green solvents with different polarity degrees. The inhibition activity of all the obtained extracts on both α-glucosidase and α-amylase was evaluated and the highest activity was observed for the EtOAc extract which showed an IC50 value about 60-fold lower than the reference compound 1-deoxynojirimycin (DNJ) on α-glucosidase (0.42 ± 0.02 and 25.4 ± 0.6 µg/mL, respectively). As expected on the basis of the literature data concerning both α-glucosidase and α-amylase inhibition, a milder inhibition activity on pancreatic α-amylase was observed. Preliminary in vivo tests on Drosophila melanogaster carried out on the most active obtained extract (EtOAc) confirmed the in vitro observed hypoglycemic activity. Finally, the EtOAc extract metabolic profile was determined through GC-MS and HRMS analyses.

Nutritional contents and antimicrobial activity of the culinary-medicinal mushroom Leccinum scabrum

ABSTRACT Leccinum scabrum (Bull.) Gray (Boletaceae) is an edible mycorrhizal species with potential application interest due to its food and medicinal properties. A field investigation carried out during summer in the Białowieża Primeval Forest, located along the border between Belarus and Poland allowed to collect samples for chemical composition analysis and antibacterial activity evaluation. Mushroom extracts were prepared with microwave-assisted as well as ultrasound-assisted extraction techniques (UAE and MAE). The analysis of a dry sample of L. scabrum showed a significant content of vitamins and minerals and also a remarkable content of carbohydrates, protein, dietary fibre, total sugars, total free amino acids, and polyunsaturated fatty acids. The antibacterial activity of L. scabrum aqueous extracts showed inhibitory activity against all tested bacteria. In general, MAE extract exhibits a higher inhibition activity against Listeria monocytogenes ATCC 19114. As regards the Minimum Inhibitory Concentration (MIC) values, the high antibacterial activity of MAE extract was detected for L. monocytogenes ATCC 19114 and Escherichia coli ATCC 25922. Regarding UAE, high antibacterial activity was detected for Salmonella enterica ATCC 13076 and L. monocytogenes ATCC 19114. Based on data hereby reported, L. scabrum is a culinary-medicinal mushroom with a promising potential use as a high-quality food and nutraceutical mycological resource.

Макроміцети Національного природного парку «Гуцульщина» та їх антибактеріальні властивості

The ability of ethanol extracts of 20 species of macromycetes to inhibit the growth of bacterial cultures was studied. Their influence on indicators of superoxide dismutase and catalase activities in relation to three types of test cultures of microorganisms was established. Extracts of Cantharellus cibarius, Grifola frondosa Laetiporus sulphureus, Leccinum scabrum, Mycena leptocephala, Russula rosea, Strobilomyces strobilaceus showed the largest diameters of growth retardation of microorganisms of test cultures. The ability to counteract bacteria is a species-specific feature and does not depend on the trophic timing of macromycetes, the same applies to the ability to suppress the catalase activity of microorganisms. Extracts of macromycetes, which provoked inhibition of the growth of cultures, led to changes in the SOD activity of the studied bacteria: during incubation of M. luteus culture with mushroom extracts, which showed an inhibitory effect on the growth of microorganisms with a diameter of 8-12 mm, superoxide dismutase activity increased 2-3 times, during incubation of M. luteus bacteria with extracts that inhibited growth with a diameter greater than 12 mm, the enzymatic activity increased 8-10 times. The SOD activity of B. spizizeni increased by 40-80% at the 24th hour of the experiment with extracts, the use of which is accompanied by the formation of a 10 mm zone of growth inhibition, but already after 48 hours of the experiment, the indicators of the SOD activity of B. spizizeni decreased to the control values. SOD activity of E. coli increased on the 24th hour of the experiment by 1.4-1.6 times relative to the control values, even with the use of extracts that provoked inhibition of the growth of cultures with a diameter of 8 mm and remained high on the 48th hour of the experiment (in 1.2-1.3 times).

Current 137Cs accumulation by mushrooms in different site types of scots pine forests of Ukrainian Polissia

The study of 137Cs radioactive contamination of fruiting bodies of mushrooms was carried out in September - October 2022 in 18 sampling sites laid out in the Korosten district of Zhytomyr region in 3 of the most widespread forest site types (FST): fresh infertile pine site type (А2), fresh fairly infertile pine site type (В2) and moist fairly infertile pine site type (В3). It was found that the highest levels of 137Cs content in investigated FST were characteristic of symbiotrophic mushroom species - Сortinarius mucosus, Сortinarius caperatus, Sarcodon imbricatus, Imleria badia, Tricholoma equestre, Paxillus involutus, Hygrophorus hypothejus, and the lowest - for xylotrophes-saprotrophes such as Armillaria mellea and Tapinella atrotomentosa. When analyzed, it was shown that interspecific differences of average values of 137Cs aggregated transfer coefficient (Tag) among mushrooms in each FST varied in a wide range: they reached 1.1⋅103 times in FST-A2 - from 435 in Cortinarius mucosus to 0.4 m2⋅kg-1⋅10-3 in Armillaria mellea; 71.4 times - in FST-B2 - from 162 in Sarcodon imbricatus to 2.3 m2⋅kg-1⋅10-3 in Armillaria mellea; and 12 times - in FST-B3 - from 111 in Imleria badia to 9.2 m2⋅kg-1⋅10-3 in Leccinum scabrum. Also, it was shown that in genus Russula even in the same FST-B2 among five studied species a 24-fold change in average values of 137Cs Tag are observed - from 67 in Russula vinosa to 2.8 m2⋅kg-1⋅10-3 in Russula aeruginea. The results of ANOVA were discussed as well as the essentiality of the difference of the average values of Tag in mushroom species in different FST.

The Role in the Human Diet of Bioaccumulation of Selenium, Copper, Zinc, Manganese and Iron in Edible Mushrooms in Various Habitat Conditions of NW Poland—A Case Study

The aim of the study was to determine the contents of microelements in Boletus edulis, Imleria badia and Leccinum scabrum, taking into account the soil conditions in selected forest areas of Northwest Poland and the bioaccumulation capacity of these fungi and their role in the human diet. Se, Cu, Zn, Mn and Fe contents were determined in the soil (organic and mineral layers) and mushrooms. The study showed that the soils on which fruiting bodies grew did not differ significantly in the contents of these trace elements. The concentrations of microelements in mushrooms in NW Poland were mostly at the lower range of the contents reported for these species in other regions of Poland and Europe. The uptake of microelements by the studied mushrooms was influenced by soil reaction, organic matter content, and bioavailable and total forms of the elements. B. edulis contained significantly more Se than other mushroom species and, together with I. badia, was much more abundant in Cu and Zn than L. scabrum. Fruiting bodies bioaccumulated Se (most strongly by B. edulis; BCF = 120.6), Cu and Zn. The contents of microelements in the tested mushrooms may be supplementary elements in the human diet.

MERCURY CONTENT IN THE WILD EDIBLE LECCINUM MUSHROOMS GROWING IN SLOVAKIA: ENVIRONMENTAL AND HEALTH RISK ASSESSMENT

The samples of 4 mushroom species from the genus Leccinum ((Leccinum pseudoscabrum (Kallenb.) Šutara, Leccinum scabrum (Bull.) Gray, Leccinum duriusculum (Schulzer ex Kalchbr.) Singer, and Leccinum albostipitatum den Bakker & Noordel) were picked from 6 forested areas of Slovakia in 2020 (Čačín – Jelšovec, Hliník and Hronom, Kurima – Taraš, Mníšek nad Popradom, Snina – Štefekovo, Žákylské pleso). Total mercury content in soil and mushroom parts was determined by cold-vapor AAS analyzer AMA 254. Mercury content in monitored soil samples ranged from 0.07 to 0.18 mg.kg-1 DM. The limit value for Hg in soil ( 0.50 mg.kg-1) was not exceeded. Mercury content in analyzed cap samples ranged from 0.41 to 7.52 mg.kg-1 DM. Mercury content in analyzed stem samples ranged from 0.40 to 2.91 mg.kg-1 DM. The EU limit value in edible mushrooms for Hg (0.75 mg kg-1 FW) was exceeded in caps from Mníšek nad Popradom. Values of the bioconcentration factor indicate that Leccinum mushrooms are accumulators of Hg. The PTWI of Hg was not exceeded. The THQ of all samples was lower than 1. This means that the average consumption of mushrooms from this localities should not cause a serious risk to human health. However, the intake of Hg from other sources must also be taken into account.

Occurrence, bio-concentration and distribution of rare earth elements in wild mushrooms

Using validated methodology, this study explores the bioconcentration potential and status of rare earth elements (REE) and yttrium (Y) in wild mushrooms collected from Belarus, China and Poland and in the associated forest topsoil. Baseline data for REE and Y distributions in the morphological parts of the fruiting bodies of Caloboletus calopus, Cantharellus cibarius, Craterellus cornucopioides, Imleria badia, Laccaria amethystina, Lactifluus piperatus, Leccinum scabrum and Suillus grevillei are presented. REE were in the range of 14 to 42 mg kg−1 dw in forest topsoil and from 35 to 48 mg kg−1 dw in profiled soil layers from the Sobowidz site in Poland. Forest topsoil sampled in Belarus contained 67 mg kg−1 dw. Yttrium concentrations in soil ranged from 2.9 to 10 mg kg−1 dw. The median REE concentration in wild mushrooms was around 200 μg kg−1 dw (20 μg kg−1 fresh weight). This implies negligible dietary intake even for high level consumers. The bioconcentration factors (BCF) of individual REE and Y ranged from 0.0002 to 0.0229, showing bio-exclusion. The BCF tended to be similar for groups of REE (La to Tb and Dy to Lu) depending on the mushroom species and site. REE from Dy to Lu were better bioconcentrated than those from La to Tb. The similarity of the BCFs of individual REE by species at a given site implies the same absorption pathway, although a lower concentration in the topsoil favoured bioconcentration. REE and Y concentrations varied between species as well as within the same species between sites. Their accumulation in mushrooms appears to reflect condition at the site of collection, and may also be species-specific but confirming this would require further investigation of different species, topsoils and sites.

MACROMYCETES AS BIOINDICATORS OF RADIONUCLIDE CONTAMINATION IN FOREST ECOSYSTEMS

The aim of the paper is to determine the specific activity of radionuclides in macromycetes in Ulyanovsk region forest ecosystems exposed to radiation after the Chernobyl accident.
 Materials and Methods. The fruiting bodies of macromycetes (soil and wood-destroying fungi) gathered in the radioactive contaminated territories served as the material for the research. The authors determined the specific activity of Cs-137, K-40, Ra-226, Th-232 radionuclides. The study was conducted during the growing season in the Ulyanovsk region forests (Inza and Karsun districts). For comparison, fungi samples (control) were also gathered in the Novospassky region, where the excess background radiation was not observed. Trial sites with similar forest growth conditions were selected. The specific activity of radionuclides in the fungi fruiting bodies was determined according to Guidelines 2.6.1.1194–03.
 Results. In soil fungi, the highest average Cs-137 specific activity was found in the fruiting bodies of Leccinum scabrum, K-40 and Ra-226 specific activity – in the fruiting bodies of Suillus luteus, and Th-232 specific activity – in the fruiting bodies of Macrolepiota procera.
 In wood-destroying fungi, the highest average Cs-137, K-40 and Ra-226 specific activity was observed in the fruiting bodies of Fomitopsis pinicola, and the highest Th-232 specific radioactivity – in the fruiting bodies of Coriolus versicolor. The average specific activity of all radionuclides in the mushrooms from the radioactive contamination territories (Inza and Karsun districts) was mainly higher than in the control (Novospassky district). In the studied samples of soil and wood-destroying fungi, excess radioactive concentrations were not found.
 Conclusion. Forest mycobiota can be used as a bioindicator of radioactive environmental pollution.

Radioactive Contamination of Wild Mushrooms from Ukraine under Conditions of Contrasting Radiation Loads: 36 Years after the Chernobyl Nuclear Power Plant Catastrophe.

It has been 36 years since the Chernobyl Nuclear Power Plant catastrophe, but the consumption of wild mushrooms in Ukrainian Polissya is still dangerous since its territory is heavily contaminated by radionuclides. The aim of this study was to estimate 137Cs and 90Sr uptake in wild mushrooms from locations with contrast radioactive loads (Zhytomyr Polissya, Ukraine). In mushroom samples collected from Drevlyanskyi Nature Reserve since 2013 (average levels of soil surface contamination with 137Cs are 555-1480 kBq m-2), the highest levels of 137Cs were observed in symbiotroph species - Imleria badia (≤ 2680 kBq kg-1 dry mass [dm]), Tricholoma equestre (≤ 1420 kBq kg-1 dm), Lactarius rufus (≤ 602 kBq kg-1 dm), Sarcodon imbricatus (≤ 464 kBq kg-1 dm), Leccinum scabrum (≤ 117 kBq kg-1 dm), Suillus bovinus (≤ 118 kBq kg-1 dm), and Boletus edulis (≤ 96 kBq kg-1 dm). 90Sr activity was significantly lower, with the highest levels detected in Russula emetica (193 Bq kg-1 dm), Daedaleopsis confragosa (145 Bq kg-1 dm), and Hypholoma fasciculare (141 Bq kg-1 dm). The 137Cs/90Sr ratio in fruiting bodies in samples ranged from 6.1 (Bovistella utriformis) to 28,979 (T. equestre). Activity concentrations in mushrooms from locations with relatively low contamination with 137Cs (18.5-27.75 kBq m-2) also reached the highest values in symbiotroph species I. badia (7698 Bq kg-1 dm), Lactarius vellereus (6072 Bq kg-1 dm), and S. luteus (1448 Bq kg-1 dm). Potential calculated annual effective doses due to mushroom consumption by adults, considering only the effect of 137Cs, reached 0.311 and 8.71 mSv in B. edulis and I. badia from highly contaminated locations, respectively, and 0.0014 and 0.009 mSv in these species from low contaminated ones.

Bioaccumulation of Macronutrients in Edible Mushrooms in Various Habitat Conditions of NW Poland-Role in the Human Diet.

Recently, the interest in mushroom consumption has been growing, since their taste and low calorific value are appreciated, but also due to their nutritional value. In determining the usefulness of mushrooms in the human diet, it is important to consider the conditions of their occurrence to perform the assessment of bioaccumulation of minerals. The aim of the study was: (a) to determine the content of selected macronutrients (P, K, Ca, Mg, Na) in fruiting bodies of Boletus edulis, Imleria badia, Leccinum scabrum and the soils, (b) to determine their bioaccumulation potential taking into account the habitat conditions, and (c) an attempt to estimate their role in covering the requirement for macronutrients of the human organism. The research material was obtained in the NW of Poland: Uznam and Wolin, the Drawa Plain and the Ińsko Lakeland. In the soil, we determined the content of organic matter, pH, salinity and the content of absorbable and general forms of macronutrients. The content of macronutrients in mushrooms was also determined. Chemical analyses were performed using the generally accepted test methods. The study showed that in NW Poland, B. edulis grew on the acidic soils of Arenosols, and I. badia and L. scabrum grew on Podzols. The uptake of K, Mg and Ca by the tested mushrooms was positively, and P and Na negatively correlated with the content of these elements in the soil. The acidity of the soil affected the uptake of K and Mg by mushrooms. There was no effect of the amount of organic matter in the soil noticed on the content of macronutrients (except sodium) in mushrooms. Among the studied macronutrients, none of the mushrooms accumulated Ca, while P and K were generally accumulated in the highest amounts, regardless of the species. Each of the other elements was usually accumulated at a similar level in the fruiting bodies of the species we studied. The exception was I. badia, which accumulated higher amounts of Mg compared to B. edulis and L. scabrum. Mushrooms can enrich the diet with some macronutrients, especially in P and K.

Effect of drying, blanching, pickling and maceration on the fate of 40K, total K and 137Cs in bolete mushrooms and dietary intake

The effects of blanching, blanching and pickling and maceration on the leaching of 137Cs and 40K from the flesh of three edible bolete mushroom species—Boletus edulis, Leccinum scabrum and Leccinum versipelle—were investigated. Significant (p < 0.05) decreases in activity were observed but varied depending on the treatment. Relative to fresh mushrooms, blanching decreased the activity concentration of 137Cs by 15 ± 13%, and of 40K, by 16 ± 7%, but blanching and pickling (vinegar) reduced activity more effectively, by 55 ± 8% and 40 ± 20% respectively. The corresponding losses of 137Cs and 40K through maceration of dried, powdered mushrooms were 38 ± 11% and 35 ± 14% ww, respectively. These results indicate that traditional domestic processing methods may not be as efficient at excluding 137Cs radioactivity as shown in some other studies. The activity concentration of 137Cs in a typically sized (100 g) portion of a processed mushroom (sourced from nearshore regions of the southern Baltic Sea coast near Gdańsk in 2015) meal was projected to be low, i.e. in the range of 0.51 to 12 Bq kg−1 ww. The corresponding effective dose of 137Cs from blanched, blanched and pickled and macerated mushrooms per capita was also assessed to be low, from 0.001 to 0.010 μSv. Nutritionally, the median concentration of potassium (330 mg) in 100 g portions of blanched or pickled mushrooms would account for around 7% of the adequate adult daily intake.Graphical abstract

Detrimental and essential elements in fruiting bodies of mushrooms with ecological relationship to birch (Betula sp.) collected in the Bohemian Forest, the Czech Republic.

Four macrofungi species with ecological relationship to birch (Betula sp.) were analyzed with regard to the content of 21 detrimental and essential elements in their fruiting bodies. Two of the species (Leccinum scabrum and Leccinum versipelle) were mycorrhizal forming edible fruiting bodies while the others (Fomitopsis betulina and Inonotus obliquus) parasitic used in alternative medicine. The samples were collected near the town of Vimperk in the Bohemian Forest, the Czech Republic. L. scabrum accumulated Ag, Rb, Cd, Cs, Se, Cu, and Zn with bioconcentration factors of 22, 5.5, 4.7, 3.2, 2.0, 1.7, and 1.4, respectively. Similar trend in bioconcentration was revealed for L. versipelle with bioconcentration factors of 28, 5.2, 2.4, 1.9, 1.6, and 1.6 determined for Ag, Rb, Cu, Se, Zn, and Cd, respectively. Considerably high contents of Ca (400 ± 190 mg kg-1 dry matter), Fe (110 ± 30), Mg (1100 ± 300), and Zn (220 ± 90) were found in F. betulina. Similarly, high contents of Ca (1000 ± 700 mg kg-1 dry matter), Fe (110 ± 30), Mg (2000 ± 1100), Mn (410 ± 270), Rb (160 ± 60), and Zn (140 ± 70) were determined for I. obliquus. The different lifestyles (mycorrhizal vs. parasitic) were reflected in 13 significant differences of element contents.

Stężenie rtęci w grzybach gatunku koźlarz babka i muchomor czerwony zebranych na terenie Polski

Stężenie rtęci w grzybach gatunku koźlarz babka i muchomor czerwony zebranych na terenie Polski

Distribution and bioconcentration of some elements in the edible mushroom Leccinum scabrum from locations in Poland

The element concentrations in the fruitbodies of Leccinum scabrum from two forested upland sites and one lowland site of different geochemical background were compared to topsoil concentrations. The aim of the study was to establish baseline concentration datasets, gain insight into the species' bioconcentration potential and to assess the impact of anthropogenic factors. The validated methods for analysis include inductively coupled plasma-atomic emission spectroscopy (ICP-AES) and cold-vapor atomic absorption spectroscopy (CV–AAS). Bioinclusion (bioconcentration factor > 1) by L. scabrum was observed for the elements Ag, Cd, Cu, K, Hg, Mn, Na, Mg, P, Rb, and Zn. In contrast, the elements Al, Ba, Ca, Fe, Ni, and Sr as well as the toxic Pb were bioexcluded. Among these elements, the toxic elements Cd and Pb are noteworthy regarding the aspect of human mushroom consumption. The medians of Cd in caps of L. scabrum from the upland sites were in the range of 5.6–6.6 mg kg−1 dm, with a maximum in an individual sample of 14 mg kg−1 dm, which is in the range of concentrations reported previously for polluted soils. Lead concentrations were much lower, with medians in the range of 0.79–1.3 mg kg−1 dm in caps and 0.48–0.59 mg kg−1 dm in stipes. Mineral contents of L. scabrum appear to be the result of a complex interaction of a species’ characteristic physiology with local mineral soil geochemistry and with anthropogenic pollution factors.

Toxicological risks and nutritional value of wild edible mushroom species -a half-century monitoring study

The content of major- and trace elements in wild-growing mushrooms has been subject to numerous studies, but the data on long-term trends in this regard are scarce. The aim of research was to determine the content of 34 elements in four edible mushroom species Boletus edulis, Imleria badia, Leccinum scabrum and Macrolepiota procera, and associated soil collected from Polish forests between 1974 and 2019. As initially hypothesized, the element concentration in the studied soil revealed an increasing trend and was positively correlated with their levels found in fruit bodies. Bioconcentrafion Factor values exceeding 1 were documented for all mushroom species for K, P, Ag, Cd, Cu, Hg, and Zn. When compared to the Adequate Intakes, all the mushroom species were found to be a good dietary source of K, P, and Zn (range of 6260–8690, 6260–8690 and 97–135 mg kg−1 dry weight (dw), respectively), and B. edulis and I. badia a moderate source of Fe (mean 71.5 and 76.5 mg kg−1 dw, respectively), B. edulis of Mn and Mo (mean 20.0 and 0.42 mg kg−1 dw, respectively), while L. scabrum and M. procera a source of Cu. Consumption of the studied mushrooms would not lead to significant exposure to Al, As, Cr, or Ni. Considering that wild mushrooms will continue to be collected in Poland, one should bear in mind that they are a limited source of minerals in the human diet while their frequent, regular consumption, associated with exposure to selected toxic elements, should not be recommended.

Productivity of edible fungi in taiga communities

The authors studied edible macrofungi in natural forest communities of middle taiga in Kirov region, Russia. Out of more than 400 macrofungi species known within the area, 5 are rare and being protected, over 20 are used as medicinal, 149 species are considered edible, of which 46 are legally allowed for collection. Local population mostly collects 10-15 species. The largest species variety of economically important macrofungi is found in cowberry and maianthemum-cowberry forest types in which Boletus edulis, B. piniphilus, Leccinum scabrum, L. aurantiacum, L. versipelle, Suillus variegates, Xerocomus subtomentosus, Lactarius rufus, Cantharellus cibarius dominate. Maximum long-term average annual productivity of fruit bodies is marked in young pine lichen forests (127.1 kg/ha), minimum - in mature and overmature bilberry and maianthemum-bilberry spruce forests (24.0 kg/ha). In years of good yield basic dominating species of edible fungi in middle taiga are Suillus granulatus and S. luteus (productivity up to 28-57 kg/ha), Cantharellus cibarius (11-17 kg/ha) and red-cap bolete (up to 21 kg/ha).

Accumulation of Minerals by Leccinum scabrum from Two Large Forested Areas in Central Europe: Notecka Wilderness and Tuchola Forest (Pinewoods).

Leccinum scabrum sporocarps and associated topsoils from two areas in Poland have been characterized for contents and bioconcentration potential of Ag, Al, Ba, Ca, Cd, Co, Cu, Fe, Hg, K, Mg, Mn, Na, Ni, P, Pb, Rb, Sr and Zn. Topsoil and fruitbody element composition varied between the two study sites, most likely as a result of local soil geochemistry. Element content of the labile fraction in topsoil from both sites followed the 'pseudo-total' fraction and median values (mg kg-1 dry matter) were: K 380 and 340, Mg 760 and 840, P 1100 and 920, Al 3800 and 8100, Ag 0.31 and 0.28, Ba 28 and 37, Ca 920 and 790, Cd 0.23 and 0.23, Co 2.0 and 1.7, Cu 3.2 and 3.6, Fe 2800 and 6300, Mn 280 and 180, Na 99 and 110, Ni 7.8 and 8.8, Pb 12 and 18, Rb 1.3 and 2.1, Sr 4.8 and 4.0 and Zn 22 and 19, respectively. Only for some elements such as K, Mg, Al, Ag, Ca, Co, Mn, Na, Ni, Sr and Zn we found concentration differences between the two study sites for the caps of sporocarps. With the exception of Al, Mn, Na and Pb, stipes showed a similar tendency. Caps had a higher concentration of K, Rb, P, Mg, Al, Ag, Cu, Fe, Zn, Cd, Pb and Ni compared to stipes, while Na, Ba and Sr contents were higher in stipes. The comparison of soil and fruitbody concentrations indicates that L. scabrum bioconcentrate some elements while others are bioexcluded.

Accumulation of Heavy Metals by Fungi in the Cities of Central Black Earth Region

Emissions of heavy metals into the environment lead to their accumulation in living organisms. This article presents the data on the accumulation of heavy metals in the fungi fruit in urban ecotopes of cities in the Western part of the Central Black Earth region. The features of accumulation of heavy metals by macromycetes of different taxonomic affiliation and ecological specialization under multi-level anthropogenic burden are studied. It was noted that MAC of Cadmium (Cd) in edible fungi can be in large excess over 88 times, lead (Pb) – 87 times, copper (Cu) – 6.8 times, zink (Zn) – 6.1 times. The accumulation of heavy metals by macromycetes can be determined by the degree of soil pollution, the bioavailability of heavy metals, as well as environmental specialization of fungi. The highest concentrations of heavy metals were recorded in the fruit bodies of soil and litter saprophytes. Cantharellus cibarius (L.), Murrill, Agaricus melleus (Vahl), Agaricus equestris L. were universal accumulators of Cd, Zn and Cu. Leccinum scabrum (Bull.) Gray, Kuehneromyces mutabilis (Schaeff.) Singer & A.H. Sm., Myxocollybia velutipes (Curtis) Singer are defined as selective Cd accumulators.

Multiannual monitoring (1974–2019) of rare earth elements in wild growing edible mushroom species in Polish forests

There is a growing demand for rare earth elements (REEs) due to their use in modern technologies, and this may result in their emission to the environment. This is the first long-term study to monitor the content of REEs in four edible mushroom species. Over 21,900 samples of fruit bodies (sporocarps) of Boletus edulis, Imleria badia, Leccinum scabrum and Macrolepiota procera and their underlying soils, collected between 1974 and 2019 from 42 forest sites in Poland were examined in an attempt to understand the time evolution of the presence of REEs in the environment. In general, I. badia and B. edulis displayed a greater total content of REEs on mg per kg basis than L. scabrum and M. procera. A gradual increase in REEs in the studied mushrooms as well as associated forest soil samples was observed over the monitored period. Both levels were also highly correlated. Regardless of the considered period, human consumption of these mushrooms would not contribute significantly to dietary exposure to REEs. Wild growing mushroom species studied over a long time period may be a good bioindicator of REE migration to the environment.

EVALUATION OF THE EFFECT OF WATER ON CULINARY PROCESSING OF MUSHROOMS ON CONCENTRATION IN ZINC AND COPPER

The influence of water without mineral residue on the concentration in fungi of micronutrients during their culinary processing was investigated. It was found that the concentration of zinc in the mushrooms decreased from 1,18 to 1,76 times by soaking them for 1 day in water without mineral residue (boiled water from which the mineral residue was removed). In particular, the least decrease in zinc concentration was observed in mushrooms Gyroporus cyanescens (Lat.), and the greatest decrease in mushrooms Armillaria mellea (Lat.). In other species of deaths the following decrease in zinc concentration was observed: Cantharēllus cibārius (Lat.) — by 1,22 times, Laetiporus sulphureus (Lat.) — by 1,2 times, Butyriboletus regius (Lat.) — by 1,23 times, Leccinum (Lat.) — by 1,2 times, Russula Pers. (Lat.) — by 1,2 times, Boletus edulis (Lat.) — by 1,21 times, Amanita rubescens (Lat.) — by 1,21 times, Leccinum scabrum (Lat.) — by 1,2 times, Leccinum aurantiacum (Lat.) — by 1,21 times. From 1,09 to 1,5 times a decrease in zinc concentration for soaking mushrooms during the 1 day in water without mineral residue (distilled) was observed. The smallest decrease in zinc concentration was observed in mushrooms Gyroporus cyanescens (Lat.), and the greatest decrease in mushrooms Armillaria mellea (Lat.), as in the first variant. Other types of mushrooms showed the following indicators: Cantharēllus cibārius (Lat.) — by 1,12 times, Laetiporus sulphureus (Lat.) — by 1,12 times, Butyriboletus regius (Lat.) — by 1,12 times, Leccinum (Lat.) — by 1,1 times, Russula Pers. (Lat.) — by 1,12 times, Boletus edulis (Lat.) — by 1,1 times, Amanita rubescens (Lat.) — by 1,14 times, Leccinum scabrum (Lat.) — by 1,11 times, Leccinum aurantiacum (Lat.) — by 1,14 times. The concentration of copper in the mushrooms decreased from 4 to 11 times by soaking them for 1 day in water without mineral residue (boiled water from which the mineral residue was removed). In particular, the smallest decrease in copper concentration was observed in mushrooms Armillaria mellea (Lat.), and most — in mushrooms Amanita rubescens (Lat.), and other mushrooms had such indicators — Cantharēllus cibārius (Lat.) — by 11 times, Gyroporus cyanescens (Lat.) — by 10,7 times, Laetiporus sulphureus (Lat.) — by 5 times, Butyriboletus regius (Lat.) — by 6 times, Leccinum (Lat.) — by 8,5 times, Russula Pers. (Lat.) — by 11 times, Boletus edulis (Lat.) — by 9 times, Leccinum scabrum (Lat.) — by 9,6 times, Leccinum aurantiacum (Lat.) — by 9 times. From 1,8 to 2,5 times the concentration of copper for soaking mushrooms during the 1day in water without mineral residue (distilled) decreased: the smallest decrease in the concentration of copper was observed in mushrooms Leccinum aurantiacum (Lat.), and the largest decrease — in mushrooms Laetiporus sulphureus (Lat.). And in other kinds of mushrooms the following indicators are noted: Cantharēllus cibārius (Lat.) — by 2 times, Gyroporus cyanescens (Lat.) — by 2,15 times, Butyriboletus regius (Lat.) — by 2 times, Leccinum (Lat.) — by 2,12 times, Russula Pers. (Lat.) — by 2,09 times, Boletus edulis (Lat.) — by 2 times, Amanita rubescens (Lat.) — by 1,83 times, Leccinum scabrum (Lat.) — by 2,18 times, Armillaria mellea (Lat.) — by 2.12 times.