Leaf Number Research Articles

Optimum intra-row spacing and clove size for the economical production of garlic (Allium sativum L.) in Northwestern Highlands of Ethiopia

Abstract Garlic is one of the most important cash crops cultivated by smallholder farmers throughout Ethiopia. However, due to inappropriate agronomic practices including clove size and intra-row spacing, the productivity of the crop is low in the country. Therefore, a field experiment was conducted during the 2019 main cropping season under rain feed conditions to identify the optimum clove size and intra-row spacing for the economical production of garlic in Lay Gayint district, Northwestern Ethiopia. Local garlic cultivar obtained from farmers was used as a test crop. A factorial combination of three clove sizes and four intra-row spacings was used in a randomized complete block design with three replications. The results revealed that the main as well as the interaction effects of clove size and intra-row spacing influenced most of the tested growth and yield parameters of garlic. The longest plant height (73.77 cm) was recorded from garlic plants grown at 5 cm intra-row spacing, while the shortest plant height (66.98 cm) was from plants grown at 12.5 cm. The treatment combination of large-sized clove and 12.5 cm intra-row spacing recorded the longest leaf length (61.07 cm), the highest leaf number (7.79), bulb diameter (4.98 cm), bulb weight (33.83 g), and clove weight (2.71 g) of garlic. However, the treatment combination of large-sized cloves and 7.5 cm intra-row spacing produced the highest marketable (13.44 t ha−1) bulb yield. Based on the partial budget analysis, however, medium-sized cloves (1.5–1.99 g) planted at 10 cm intra-row spacing recorded the highest net benefit (723,205 Eth-Birr ha−1) with an acceptable marginal rate of return (3277.3%). Therefore, growing garlic using medium-sized cloves (1.5–1.99 g) at 10 cm intra-row spacing is recommended for economical production of garlic by smallholder farmers in the study area and areas with similar agroecology.

Allicin and total phenolic content in ramps (Allium tricoccum Ait.) in relation to phenological stage, morphological traits, and harvest location

Ramps (Allium tricoccum Ait., Alliaceae/Amaryllidaceae) are an herbaceous perennial native to the forests of central/eastern North America. Ramps are consumed for their unique onion and garlic flavor. Knowledge of ramp phytochemistry is limited. Here the influence of plant part, phenological stage, morphology, and growing location on allicin and total phenolic content (TPC) in ramps was examined. Ramps were collected from wild populations across six sites in Pennsylvania at seven developmental stages. In spring, when leaves were present, allicin levels were 5 times greater in bulbs than leaves, and TPC in leaves was 4.5 times greater than bulbs. Allicin concentration was influenced by phenology and peaked at flowering in bulbs and at peak stage in leaves. TPC in bulbs and leaves was influenced by phenology and harvest location. TPC concentration was highest in bulbs and leaves at flowering and emergence, respectively. Stem color and leaf number had no influence on the phytochemicals measured.

The influence of foliar macronutrient on growth and yield of sweet corn grown in Rengam and Rasau soil series under greenhouse conditions

The purpose of this study was to determine the effects of different rates of macronutrients as a foliar applications on growth and yield performance of sweet corn cultivated in Rengam and Rasau soil series of Malaysia under greenhouse condition. The study comprised five treatments of macronutrient fertilization as a foliar application (0, 25, 50, 75 and 100 % NPK). The plant height, stem width, leaf number, leaf area and cob height of sweet corn grown in Rengam soil series were significantly influenced by foliar macronutrient application. Notably, a rise in cob weight of sweet corns cultivated in Rasau soil series was observed at the 75 % rate, reaching 75 g, compared to 68 g for sweet corn grown in Rengam soil series. The foliar application of 100 % NPK resulted in the highest total leaf area at 252.92 cm2 compared to all other treatments. Regarding the cob fresh weight and dry matter content of sweet corn cultivated in the Rasau soil series, significant improvement noted with the 75 % NPK treatment, however plants grown in the Rengam soil series and treated with 75 % NPK as a foliar application did not exhibit significant effects. The concentrations of Fe and Mn in the corn stem from both soil series significantly increased with macronutrients foliar application. In the corn leaves grown in Rengam soil series, the concentration of nutrients was significantly influenced by the rate of NPK used as a foliar application in this study. Notably, the increased of Ca content in the treated leaves was significantly higher compared to control plants. Additionally, the contents of P, K, Cu and Zn in the corn flower of the plants in the Rengam soil series were significantly elevated in treated plants compared to untreated ones. Conclusively, based on this greenhouse study, it can be inferred that two foliar sprays of macronutrient solution at rates of 75 % and 100 % NPK greatly enhanced the growth, yield and nutrient contents of sweet corn cultivated in the Rengam and Rasau soil series.

Integrated metabolomic and metagenomic strategies shed light on interactions among planting environments, rhizosphere microbiota, and metabolites of tobacco in Yunnan, China

Changes in climatic factors and rhizosphere microbiota led plants to adjust their metabolic strategies for survival under adverse environmental conditions. Changes in plant metabolites can mediate crop growth and development and interact with rhizosphere microbiota of the plant rhizosphere. To understand the interactions among environmental factors, rhizosphere microbiota, and metabolites of tobacco, a study was conducted by using integrated metagenomic and metabolomic strategies at four typical representative tobacco planting sites in Yunnan, China. The results showed that the agronomical and biochemical traits were significantly affected by temperature, precipitation (PREP), soil pH, and altitude. Correlation analyses revealed a significant positive correlation of temperature with length, width, and area of the leaf, while PREP correlated with plant height and effective leaf numbers. Furthermore, total sugar and reducing sugar contents of baked leaves were significantly higher, while the total nitrogen and total alkaloid levels were lower in tobacco leaves at site with low PREP. A total of 770 metabolites were detected with the highest number of different abundant metabolites (DMs) at Chuxiong (CX) with low PREP as compared to the other three sites, in which secondary metabolites were more abundant in both leaves and roots of tobacco. A total of 8,479 species, belonging to 2,094 genera with 420 individual bins (including 13 higher-quality bins) harboring 851,209 CDSs were detected. The phyla levels of microorganisms such as Euryarchaeota, Myxococcota, and Deinococcota were significantly enriched at the CX site, while Pseudomonadota was enriched at the high-temperature site with good PREP. The correlation analyses showed that the metabolic compounds in low-PREP site samples were positively correlated with Diaminobutyricimonas, Nissabacter, Alloactinosynnema, and Catellatospora and negatively correlated with Amniculibacterium, Nordella, Noviherbaspirillum, and Limnobacter, suggesting that the recruitment of Diaminobutyricimonas, Nissabacter, Alloactinosynnema, and Catellatospora in the rhizosphere induces the production and accumulation of secondary metabolites (SMs) (e.g., nitrogen compounds, terpenoids, and phenolics) for increasing drought tolerance with an unknown mechanism. The results of this study may promote the production and application of microbial fertilizers and agents such as Diaminobutyricimonas and Alloactinosynnema to assemble synthetic microbiota community or using their gene resources for better cultivation of tobacco as well as other crops in drought environments.

Vegetative propagation of Phytolacca acinosa Roxb. by rhizome cuttings: a step towards conservation and cultivation approach

IntroductionPhytolacca acinosa Roxb. is a highly valuable multipurpose herb native to the Himalayan region. Unsustainable harvesting of this species due to its diverse uses has resulted in a rapid decline in its population across natural habitats, thereby necessitating its propagation and conservation. To overcome this challenge, the potential of P. acinosa rhizomes for ex situ regeneration was evaluated.MethodsThe current study aims to develop a standard propagation protocol for P. acinosa. Rhizome cuttings derived by splitting whole rhizomes were used to study the effect of various hormones and soil compositions on their sprouting and growth performance.ResultsSoil compositions SC10 and SC5 consisting of soil, sand, pebbles, and vermicompost (1:1:1:1) and soil, sand, and vermicompost (1:1:1), respectively, were the most suitable compositions for the optimum growth of this species. The rhizome segments treated with GA3 (150ppm) induced the highest sprouting percentage (91.67%), with a minimum sprouting time of 23.25 days. The maximum root length (9.25 cm), shoot length (16.5 cm), and leaf number (11.25) were recorded for GA3 (150ppm) treated rhizome cuttings.ConclutionsOverall, the results of the present study helped in establishing a cost-effective, rapid, efficient, and simple mass propagation method for the target species. The results of this study will serve as a guide for the large-scale cultivation, effective conservation, and sustainable utilization of this economically valuable medicinal herb.

Minimum spanning tree analysis of EEG resting-state functional networks in schizophrenia

Schizophrenia is a serious and complex mental disease, known to be associated with various subtle structural and functional deviations in the brain. Recently, increased attention is given to the analysis of brain-wide, global mechanisms, strongly altering the communication of long-distance brain areas in schizophrenia. Data of 32 patients with schizophrenia and 28 matched healthy control subjects were analyzed. Two minutes long 64-channel EEG recordings were registered during resting, eyes closed condition. Average connectivity strength was estimated with Weighted Phase Lag Index (wPLI) in lower frequencies: delta and theta, and Amplitude Envelope Correlation with leakage correction (AEC-c) in higher frequencies: alpha, beta, lower gamma and higher gamma. To analyze functional network topology Minimum Spanning Tree (MST) algorithms were applied. Results show that patients have weaker functional connectivity in delta and alpha frequency bands. Concerning network differences, the result of lower diameter, higher leaf number, and also higher maximum degree and maximum betweenness centrality in patients suggest a star-like, and more random network topology in patients with schizophrenia. Our findings are in accordance with some previous findings based on resting-state EEG (and fMRI) data, suggesting that MST network structure in schizophrenia is biased towards a less optimal, more centralized organization.

Effects of calcium peroxide on growth of Pontederia cordata and water quality

Combined chemical-biological methods play an important role in ecological restoration. However, it is still unknown whether the addition of calcium peroxide (CaO2) can promote the growth of Pontederia cordata (P. cordata) and the improvement of water quality. Effects of different levels of added CaO2 (0, 0.2, 0.4, 0.6, 0.8, 1, and 2 kg/m2) on P. Cordata growth and water quality were investigated. CaO2 promoted germination and tillering and increased the leaf number (p < 0.05) of P. cordata, but reduced leaf length. The plant height and leaf width increased only when the added CaO2 levels were 0.6 kg/m2 and 1 kg/m2, respectively. Adding 0.4–0.6 kg/m2 CaO2 had a cumulative effect on the biomass. High levels of added CaO2 (2 kg/m2) affected the physiology of P. cordata resulting in the most serious damage to cell membrane lipids, the lowest catalase activity, and the highest chlorophyll content. Adding CaO2 did not cause DO and pH of the water to be too high for a long time, but excessive CaO2 made TN and CODMn too high. Thus, 1 kg/m2 CaO2 was most conducive to the growth of P. cordata and the improvement of water quality.

Optimum flow rate enhances the performance of lettuce (Lactuca sativa L.) in hydroponic culture

Poor substate flow rate affects the production of crops under hydroponic systems as contact and collision times of ions (nutrients) with roots is affected deciding nutrients uptake rate and quantity required for crop growth. Keeping in view, a study was conducted under hydroponic culture to obtain optimal flow rate for higher production of lettuce, which is an important leafy vegetable. Two lettuce varieties (Boston and Lollo Bionda) were subjected to 4 different flow rates (0.5, 1.0, 1.5, 2.0 L min-1) under completely randomized design (CRD) with factorial arrangement and replicated four times. The outcomes of the study suggest that performance of Lollo Bionda variety was better than Boston regarding root and shoot fresh and dry biomass, leaf number, area, length and width and head diameter and plant height. The same parameters kept on increasing with increase of flow rate and achieved maximum values at 1.5 L min-1. Beyond that flow rate the values for these parameters declined which indicated that the optimal flow rate for these 2 lettuce verities is 1.5 L min-1. Hence, it was concluded that for higher production of lettuce, the flow rate should be kept at 1.5 L min-1 in Nutrient Film Technique (NFT) using hydroponic system with environmental and substrate conditions mentioned in materials and method portion of this article.

Effects of different light conditions on morphological, anatomical, photosynthetic and biochemical parameters of Cypripedium macranthos Sw.

In this study, the morphological (plant height, leaf length and width, stem diameter and leaf number), anatomical (epidermal cell density and thickness, Stomatal length and width), photosynthetic (net photosynthetic rate, transpiration rate, stomatal conductance, intercellular CO2 concentration, relative humidity, leaf temperature and chlorophyll fluorescence parameters) and biochemical parameters (the content of soluble sugar, soluble protein, proline, malondialdehyde and electrical conductivity) of Cypripedium macranthos Sw. in Changbai Mountain were determined under different light conditions (L10, L30, L50, L100). The results showed that morphological values including plant height, leaf area, stem diameter and leaf number of C. macranthos were smaller under the condition of full light at L100. The epidermal cell density and epidermal thickness of C. macranthos were the highest under L30 and L50 treatments, respectively. It had the highest net photosynthetic rate (Pn) and chlorophyll content under L50 treatment. Meanwhile, correlation analysis indicated that photosynthetically active radiation (PAR) and water use efficiency (WUE) were the main factors influencing Pn. C. macranthos accumulated more soluble sugars and soluble proteins under L100 treatment, while the degree of membrane peroxidation was the highest and the plant was severely damaged. In summary, the adaptability of C. macranthos to light conditions is ranked as follows L50 > L30 > L10 > L100. Appropriate light conditions for C. macranthos are 30%-50% of full light, which should be taken into account in protection and cultivation.

Potassium fertilizer promotes the thin-shelled Tartary buckwheat yield by delaying senescence and promoting grain filling

The application rate of potassium fertilizer is closely related to the yield of crops. Thin-shelled Tartary buckwheat is a new variety of Tartary buckwheat with the advantages of thin shell and easy shelling. However, little is known about application rate of potassium fertilizer on the yield formation of thin-shelled Tartary buckwheat. This study aimed to clarify the effect of potassium fertilizer on the growth and yield of thin-shelled Tartary buckwheat. A field experiment to investigate the characteristics was conducted across two years using thin-shelled Tartary buckwheat (Miku 18) with four potassium fertilizer applications including 0 (no potassium fertilizer, CK), 15 (low-concentration potassium fertilizer, LK), 30 (medium-concentration potassium fertilizer, MK), and 45 kg·ha−1 (high-concentration potassium fertilizer, HK). The maximum and average grain filling rates; starch synthase activity; superoxide dismutase and peroxidase activities in leaves; root morphological indices and activities; available nitrogen, phosphorus, and organic matter content in rhizosphere soil; urease and alkaline phosphatase activities in rhizosphere soil; plant height, main stem node number, main stem branch number, leaf number; grain number per plant, grain weight per plant, and 100-grain weight increased first and then decreased with the increase in potassium fertilizer application rate and reached the maximum at MK treatment. The content of malondialdehyde was significantly lower in MK treatment than in other three treatments. The yields of thin-shelled Tartary buckwheat treated with LK, MK, and HK were 1.22, 1.37, and 1.07 times that of CK, respectively. In summary, an appropriate potassium fertilizer treatment (30kg·ha−1) can delay the senescence, promote the grain filling, and increase the grain weight and final yield of thin-shelled Tartary buckwheat. This treatment is recommended to be used in production to achieve high-yield cultivation of thin-shelled Tartary buckwheat.

Aeluropus littoralis stress-associated protein promotes water deficit resilience in engineered durum wheat

Global climate change-related water deficit negatively affect the growth, development and yield performance of multiple cereal crops, including durum wheat. Therefore, the improvement of water-deficit stress tolerance in durum wheat varieties in arid and semiarid areas has become imperative for food security. Herein, we evaluated the water deficiency resilience potential of two marker-free transgenic durum wheat lines (AlSAP-lines: K9.3 and K21.3) under well-watered and water-deficit stress conditions at both physiological and agronomic levels. These two lines overexpressed the AlSAP gene, isolated from the halophyte grass Aeluropus littoralis, encoding a stress-associated zinc finger protein containing the A20/AN1 domains. Under well-watered conditions, the wild-type (WT) and both AlSAP-lines displayed comparable performance concerning all the evaluated parameters. Ectopic transgene expression exerted no adverse effects on growth and yield performance of the durum wheat plants. Under water-deficit conditions, no significant differences in the plant height, leaf number, spike length, and spikelet number were observed between AlSAP-lines and WT plants. However, compared to WT, the AlSAP-lines exhibited greater dry matter production, greater flag leaf area, improved net photosynthetic rate, stomatal conductance, and water use efficiency. Notably, the AlSAP-lines displayed 25 % higher grain yield (GY) than the WT plants under water-deficit conditions. The RT-qPCR-based selected stress-related gene (TdDREB1, TdLEA, TdAPX1, and TdBlt101-2) expression analyses indicated stress-related genes enhancement in AlSAP-durum wheat plants under both well-watered and water-deficit conditions, potentially related to the water-deficit resilience. Collectively, our findings support that the ectopic AlSAP expression in durum wheat lines enhances water-deficit resilience ability, thereby potentially compensate for the GY loss in arid and semi-arid regions.

The Signed Total Mixed Roman Domination Numbers of Graphs

The problem of signed domination of graphs is a typical optimization problem. It requires that each vertex and edge be assigned a feasible label so that the sum of assigned labels is minimized. First, we propose the concept of a signed total mixed Roman domination number and introduce the related research progress. Finally, we determine several lower bounds of the signed total mixed Roman domination numbers which depend on the parameters of vertex number and edge number, degree and leaf number.

Assessing the Impact of Coated and Prilled Urea Fertilizers on Nitrogen Dynamics and Fodder Maize Yield in Alfisols

The study investigates the impact of different urea fertilizers on nitrogen mineralization, leaching losses, and growth, yield of fodder maize in Alfisols. Nitrogen is vital for plant growth but deficient in soils. Urea, a widespread nitrogen fertilizer, suffers from significant losses, prompting the development of controlled-release urea (CRU) fertilizers. This study assesses various urea formulations with coated urea and prilled urea. Laboratory incubation studies show that coated urea exhibits slower NH4+-N release, reducing losses and improving efficiency compared to uncoated urea. Nitrate content increases steadily periodically with coated urea, potentially enhancing nitrogen availability to plants. Greenhouse experiments reveal significant differences in plant height, leaf number, and leaf area among treatments. Coated urea formulations, chiefly CSPC @3%, demonstrate superior growth parameters (plant height-169.56cm, number of leaves-10.33 and leaf area-1850.59), yield (green fodder-328.25 gplant-1, dry fodder-198.61 gplant-1 and dry matter percentage-54.52) and quality parameters (crude protein-4.03, total ash content-3.82%) compared to prilled urea growth parameters (plant height-113.38cm, number of leaves-8.07 and leaf area-1670.98), yield (green fodder-246.26 gplant-1, dry fodder-116.35 gplant-1 and dry matter percentage-44.32) and quality parameters (crude protein-2.88, total ash content-2.75%), likely due to sustained nitrogen release. Higher nitrogen availability from coated urea leads to increased forage yield and quality. The findings suggest that coated urea fertilizers, specially CSPC @3% advocate improved nitrogen management, enhancing fodder maize productivity and sustainability.

Reponses of morphological and biochemical traits of bamboo trees under elevated atmospheric O3 enrichment

Dwarf bamboo (Indocalamus decorus) is an O3-tolerant plant species. To identify the possible mechanism and response of leaf morphological, antioxidant, and anatomical characteristics to elevated atmospheric O3 (EO3) concentrations, we exposed three-year-old I. decorus seedlings to three O3 levels (low O3-LO: ambient air; medium O3-MO: Ambient air+70 ppb high O3–HO: Ambient air+140 ppb O3) over a growing season using open-top chambers. Leaf shape and stomatal characteristics, and leaf microscopic structure of I. decorus were examined. The results indicated that 1) the stomata O3 flux (Fst) of HO decreased more rapidly under EO3 as the exposure time increased. The foliar O3 injury of HO and MO occurred when AOT40 was 26.62 ppm h and 33.20 ppm h, respectively, 2) under EO3, leaf number, leaf mass per area, leaf area, and stomata length/width all decreased, while leaf thickness, stomatal density, width, and area increased compared to the control, 3) MDA and total soluble protein contents all showed significantly increase under HO (36.57% and 32.77%) and MO(31.91% and 19.52%) while proline contents only increased under HO(33.27%). 4) MO and HO increased bulliform cells numbers in the leaves by 6.28% and 23.01%, respectively. HO reduced the transverse area of bulliform cells by 13.73%, while MO treatments had no effect, and 5) the number of fusoid cells interspace, the transverse area of fusoid cells interspace, and mesophyll thickness of HO significantly increased by 11.16%, 28.58%, and 13.42%, respectively. In conclusion, I. decorus exhibits strong O3 tolerance characteristics, which stem from adaptions in the leaf's morphological, structural, antioxidant, and anatomical features. One critical attribute was the enlargement of the bulliform cell transverse area and the transverse area of fusoid cells interspace that drove this resistance to O3. Local bamboo species with high resistance to O3 pollution thus need to be promoted for sustained productivity and ecosystem services in areas with high O3 pollution.

Physio-biochemical Behavior and Health Effects of Pepper Plants Subjected to Lead Stress and Their Responses to Remediating Agents as Microbial Activity and Phosphorus

This work assessed the alleviating effects of bacteria (Bacillus subtilis) and phosphorus as environmentally friendly materials on the cultivation of pepper plants in polluted soil with lead (Pb) in forms of PbSO4, and Pb(NO3)2 at rates of 0, 1000, 2000 and 3000 µg Pb/g soil. Pot experiments were conducted to study the growth parameters, some physiological factors, biochemical constituents, and yield attributes, as well as the tolerance index (TI), translocation factor (TF), bioconcentration factor (BCF), and health effects [daily intake of heavy metals (DIM), health risk index (HRI), and carcinogenic risk (CR)]. Increasing the Pb concentration of all Pb salt used in soil severely affected the plant vegetative growth parameters. In comparison with other Pb salt forms, Pb(NO3)2 salt had a strong inhibitory impact. Additionally, the photosynthetic pigments in leaves were negatively impacted by all Pb salt forms. The application of Pb in all salt forms led to changes in the leaf water deficit (LWD), osmotic pressure, and membrane integrity and decreased the total water content, relative water content (RWC), transpiration rate, and leaf succulence. Pollution with Pb salts considerably decreased the yield constituents and various chemical properties of pepper, more so in the presence of Pb nitrate than in the presence of Pb sulfate type. A comparison of the concentration of Pb presence of Pb nitrate was greatly increased than the Pb sulfate in the whole plants. The safe limit of 0.3 mg/kg was exceeded by the Pb concentration in pepper fruits (6.3 and 4.3 mg/kg) cultivated in Pb-contaminated soil [with Pb(NO3)2 and PbSO4, respectively]. Additionally, Pb sulfate had a greater detrimental effect on Pb uptake in several plant organs than other Pb salt forms. The TI of pepper plants treated with salt types was &gt;60% with PbSO4 (75.6%), whereas it was &lt;60% with Pb(NO3)2 (35.2%). The BCF values of pepper plants in the polluted Pb soils varied from 0.10 to 0.41, indicating a moderate accumulator plant. At every level of Pb contamination with all Pb salt types, the sequence of Pb TF values was as follows: roots (TFr) &gt; shoots (TFsh) &gt; fruits (TFf), with TF values &lt; 1. When compared with TFr and TFsh, TFs for shoot to fruits (TFf) had the lowest values (range, 0.07–0.22). The DIM, HRI, and CR values of pepper plants revealed that the Pb of fruit of stressed pepper plants is within safe limits. In addition to reducing the detrimental effects of intolerable Pb levels (2000 and 3000 µg Pb/g soil) on the majority of the aforementioned characters, adding Bacillus bacteria as a bio-agent and phosphorus as a chemo-agent to Pb-polluted soils also stimulated growth, increased yield, controlled plant water relations, protected photosynthetic pigments, and sharply decreased the Pb accumulation in plant organs. The Bacillus bacteria application resulted in some superior characteristics, such as root length, leaf number, leaf length, leaf area, leaf area index, fresh biomass, dry biomass, photosynthetic pigments, quantity yield attributes, reduction Pb accumulation in all plant organs, TI, TFr, TFf, BCF, in health effects trials, whereas phosphorus application improved plant height, leaf width, RWC, LWD, osmotic pressure, total soluble solids, acidity, total carbohydrates, total protein, and TFsh.

Chlorella vulgaris improves growth of Abrometiella scapigera, Abrometiella brevifolia and Abrometiella chloranta and reduces root attack by nematodes

Research objective: The aim of this study is to investigate the effects of Chlorella extract as a biofertiliser on the growth of Abrometiella spp. seedlings and to assess whether it has a repellent effect against nematodes Materials and Methods: The experiments, which began in November 2024, were conducted in the CREA-OF greenhouses in Pescia (PT), Tuscany, Italy (43°54′N 10°41′E) on Abrometiella scapigera, Abrometiella brevifolia and Abrometiella chloranta. The plants were placed in ø12 cm pots; 30 plants per thesis, divided into 3 replicates of 10 plants each. The experimental groups were: i) group without algae, irrigated with water and previously fertilised substrate; ii) group with Chlorella vulgaris, previously fertilised substrate. On 2 April 2024, plant height, leaves number, vegetative weight, root weight, root lenght, number of new plantlets, chlorophyll content, leaf area, control of the nematodes Meloidogyne javanica and Meloidogyne incognita were measured. Results and Discussion: The experiment showed that the use of Chlorella vulgaris extracts can improve the vegetative and root growth of Abrometiella scapigera, Abrometiella brevifolia and Abrometiella chloranta plants grown in pots. The treatment also led to an increase in plant height, number of leaves, length of root hairs, leaf area and number of new seedlings. The trial also showed that the use of Chlorella in plant growing media can also increase the photosynthesis rate (SPAD index) and significantly reduce the presence of nematodes, in particular Meloidogyne javanica and Meloidogyne incognita. According to some researchers, the influence of Chlorella extract on cell metabolisms is mainly due to the physiological effect of major and minor nutrients, amino acids, vitamins, and plant growth regulators on cellular metabolisms in treated plants, which increase crop yields and growth. Chlorella plants have very high photosynthesis rates, dozens of times higher than other plants. The main reason why Chlorella can grow rapidly is because it contains Chlorella Growth Factor (CGF), which is quite rich in nucleoproteins, nucleic acids, ribonucleic acids (RNA), deoxyribonucleic acids (DNA), vitamins, amino acids, polysaccharides, complex protein bodies, enzymes, glycoproteins, plant hormones, etc. Its ability to promote plant growth may be due to this factor. Conclusions: Chlorella extract significantly increased Abrometiella seedling height, leaf number and leaf area. In particular, Abrometiella seedlings treated with chlorella extract developed better root system, chlorophyll a increased significantly and the root length and number of new seedlings also improved significantly. Based on the above results that Chlorella extracts contribute to the growth and development of Abrometiella plants, we can conclude that with the treatment of Chlorella extracts, the application of chemical fertilisers in the production of succulents such as Abrometiella can be reduced.

Physiological Responses and Tolerance of Three Acacia Species to Cadmium Stress During Germination and Early Seedling Growth

Soil contamination with cadmium (Cd) is a serious threat to ecosystems. Phytoremediation is a cost-effective green technology that can be used to clean up Cd pollution, and Acacia species are potential phytoremediation agents due to their fast growth, significant biomass, and tolerance to environmental stresses. Most studies of Cd stress on Acacia focused on later vegetative stages and the tolerances during germination and early seedling are elusive. This study aimed to assess the tolerance of Acacia mangium, Acacia crassicarpa, and Acacia auriculiformis to Cd stress during germination and early seedling growth. In response to 250 μM Cd treatment, A. auriculiformis germination was slightly increased compared to the control. Leaf organ formation remained largely unaffected by Cd stress, except for the true leaf number in A. mangium, which was significantly higher in response to 125 µM Cd than those to 250 µM Cd treatment and control at 8 and 10 weeks after treatment. The onset of phyllode development transition from true leaf was significantly delayed in A. mangium compared with the other two Acacia, irrespective of the presence of Cd. There were no significant differences in leaf gas exchange parameters (photosynthesis, stomatal conductance, and transpiration), leaf-level water use efficiency, or leaf chlorophyll content between Cd-treated and control plants, suggesting that the three Acacia species are tolerant to Cd at both germination and early seedling phases. Cd accumulation during the experiment was very low in A. mangium seedlings (2.8 mg kg-1 upon 250 µM Cd) and insignificant in A. crassicarpa and A. auriculiformis, indicating non-hyperaccumulation or Cd exclusion at the seedling stage.

Genome-wide association studies identifies genetic loci related to fatty acid and branched-chain amino acid metabolism and histone modifications under varying nitrogen treatments in safflower (Carthamus tinctorius).

Effective identification and usage of genetic variation are prerequisites for developing nutrient-efficient cultivars. A collection of 94 safflower (Carthamus tinctorius ) genotypes (G) was investigated for important morphological and photosynthetic traits at four nitrogen (N) treatments. We found significant variation for all the studied traits except chlorophyll b (chl b ) among safflower genotypes, nitrogen treatments and G×N interaction. The examined traits showed a 2.82-50.00% increase in response to N application. Biological yield (BY) reflected a significantly positive correlation with fresh shoot weight (FSW), root length (RL), fresh root weight (FRW) and number of leaves (NOL), while a significantly positive correlation was also observed among carotenoids (C), chlorophyll a (chl a ), chl b and total chlorophyll content (CT) under all treatments. Superior genotypes with respect to plant height (PH), FSW, NOL, RL, FRW and BY were clustered into Group 3, while genotypes with better mean performance regarding chl a , chl b C and CT were clustered into Group 2 as observed in principal component analysis. The identified eight best-performing genotypes could be useful to develop improved nitrogen efficient cultivars. Genome-wide association analysis resulted in 32 marker-trait associations (MTAs) under four treatments. Markers namely DArT-45481731 , DArT-17812864 , DArT-15670279 and DArT-45482737 were found consistent. Protein-protein interaction networks of loci associated with MTAs were related to fatty acid and branched-chain amino acid metabolism and histone modifications.

Effect of Seed Size on Growth, Yield and Seed Quality of Jack Bean (Canavalia ensiformis (L.))

This research aimed to investigate the effect of seed size on growth, yield and seed quality of Jack beans cultivated in field conditions. The experiment was conducted at Faculty of Natural Resources and Agro-Industry, Kasetsart University Chalermphrakiat Sakonnakhon Province Campus. The experimental design was Randomized Complete Block Design (RCBD) with 3 replications and 3 treatments, and consisted of 3 different seed sizes of Jack bean (small, medium and large). The results indicated that seed size had no significant effect on growth (plant height, leaf number and weight of canopy). However, statistically significant differences (P&lt;0.05) were found in fresh and dry biomass yields, whereas large seed size had the highest biomass yield without significant difference from seed yield. The 3 sizes of jack bean gave an average seed yield of 402.07 kg/rai. In term of seed quality, it was found that all 3 sizes of jack beans seed had no significant effect on physical quality and physiological quality in both standard germinations, germination index and a number of days used for germination. However, seed sizes had a significant effect on seedling biomass weight. The large seed had the highest weight (0.62 g/plant). Therefore, this study concluded that the three seed sizes (small, medium, and large) did not affect the growth, yield and seed quality of jack beans although the large size seed had the highest biomass yield.

Effects of 5-Aminolevulinic Acid (5-ALA) on Morphological and Physiological Characteristics of Grapevine against Salt Stress

Salinity, one of the most significant abiotic stress factors restricting plant production, causes the destruction of agricultural lands and reduces productivity. In recent years, the utilization of 5-aminolevulinic acid (5-ALA) applications, which have important effects in terms of avoiding and providing tolerance to factors by impacting the physiology and metabolism of the plants, has been on the agenda. In this research, the impacts of foliar treatments of different levels of 5-ALA (0, 0.3, 0.6 and 0.9 mM) on morphological and physiological traits of 41 B American grapevine rootstocks under salinity stress (NaCl solution starting with 25 mM and reaching 150 mM concentration) were investigated. Salinity stress caused significant decreases in growth parameters, chlorophyll content, RWC and stomatal conductance, and significant increases in leaf temperature, proline and MDA content, physical damage and membrane damage degree. Under salinity stress, 0.9 mM 5-ALA treatments resulted in significant increases in shoot length (14.67 cm), root length (34.50 cm), leaf thickness (0.23 µm) leaf area (31.37 cm2), leaf number (8.67 pieces), chlorophyll content (21.83 SPAD), RWC (80.20%), proline content (0.19 μmol.g-1) and stomatal conductance (78.05 mmol.m-2.s-1); and significant decreases in physical damage degree (1.00 scale degree), membrane injury degree (15.46%) and MDA content (28.20 nmol.g-1) compared to non-ALA treatments. According to the results of this study, 5-ALA can be recommended as an alternative application to provide salinity tolerance in plants in order to reduce the damage caused by salinity stress in agricultural lands.