Leading Arm Research Articles

Magellanic System Stars Identified in SMACS J0723.3-7327 James Webb Space Telescope Early Release Observations Images

We identify 71 distant stars in James Webb Space Telescope/NIRCam early release observations (ERO) images of the field of galaxy cluster SMACS J0723.3-7327 (SMACS 0723). Given the relatively small (∼10°) angular separation between SMACS 0723 and the Large Magellanic Cloud (LMC), it is likely that these stars are associated with the LMC outskirts or the Leading Arm. This is further bolstered by a spectral energy distribution (SED) analysis, which suggests an excess of stars at a physical distance of 40–100 kpc, consistent with being associated with or located behind the Magellanic system. In particular, we find that the overall surface density of stars brighter than 27.0 mag in the field of SMACS 0723 is ∼2.3 times that of stars in a blank field with similar Galactic latitude (the North Ecliptic Pole Time Domain Field), and that the density of stars in the SMACS 0723 field with SED-derived distances consistent with the Magellanic system is ∼6.1 times larger than that of the blank field. The candidate stars at these distances are consistent with a stellar population at the same distance modulus with [Fe/H] = −1.0 and an age of ∼5.0 Gyr. On the assumption that all of the 71 stars are associated with the LMC, then the stellar density of the LMC at the location of the SMACS 0723 field is ∼740 stars kpc−3, which helps trace the density of stars in the LMC outskirts.

A Dynamically Distinct Stellar Population in the Leading Arm of the Sagittarius Stream

We present a chemical and dynamical analysis of the leading arm (LA) and trailing arm (TA) of the Sagittarius (Sgr) stream, as well as for the Sgr dwarf galaxy core (SC), using red giant branch, main-sequence, and RR Lyrae stars from large spectroscopic survey data. The different chemical properties among the LA, TA, and SC generally agree with recent studies and can be understood by a radial metallicity gradient established in the progenitor of the Sgr dwarf, followed by preferential stellar stripping from the outer part of the Sgr progenitor. One striking finding is a relatively larger fraction of low-eccentricity stars (e < 0.4) in the LA than in the TA and SC. The TA and SC exhibit very similar distributions. Considering that a tidal tail stripped off from a dwarf galaxy maintains the orbital properties of its progenitor, we expect that the e-distribution of the LA should be similar to that of the TA and SC. Thus, the disparate behavior of the e-distribution of the LA is of particular interest. Following the analysis of Vasiliev et al., we attempt to explain the different e-distribution by introducing a time-dependent perturbation of the Milky Way by the Large Magellanic Cloud's (LMC) gravitational pull, resulting in substantial evolution of the angular momentum of the LA stars to produce the low-e stars. In addition, we confirm from RR Lyrae stars with high eccentricity (e > 0.6) that the TA stars farther away from the SC are also affected by disturbances from the LMC.

The Diffuse Ionized Gas Halo of the Large Magellanic Cloud

The Large Magellanic Cloud (LMC) has an extensive Hα emission halo that traces an extended, warm ionized component of its interstellar medium. Using the Wisconsin Hα Mapper telescope, we present the first kinematic Hα survey of an extensive region around the LMC, from (ℓ, b) = (264.°5, − 45.°5) to (295.°5, − 19.°5), covering +150 ≤ v LSR ≤ + 390 km s−1. We find that ionized hydrogen exists throughout the galaxy and extends several degrees beyond detected neutral hydrogen emission as traced by 21 cm in current surveys. Using the column density structure of the neutral gas and stellar line-of-sight depths as a guide, we estimate the upper limit mass of the ionized component of the LMC to be roughly M ionized ≈ (0.6–1.8) × 109 M ☉, which is comparable to the total neutral atomic gas mass in the same region (M neutral ≈ 0.76–0.85 × 109 M ☉). Considering only the atomic phases, we find M ionized/M ionized+neutral, to be 46%–68% throughout the LMC and its extended halo. Additionally, we find an ionized gas cloud that extends off of the LMC at (ℓ, b) ≈ (285°, − 28°) into a region previously identified as the Leading Arm complex. This gas is moving at a similar line-of-sight velocity as the LMC and has M ionized/M ionized+neutral = 13%–51%. This study, combined with previous studies of the SMC and extended structures of the Magellanic Clouds, continues to suggest that warm, ionized gas is as massive and dynamically important as the neutral gas in the Magellanic System.

Radiative Turbulent Mixing Layers and the Survival of Magellanic Debris

The Magellanic Stream is sculpted by its infall through the Milky Way’s circumgalactic medium, but the rates and directions of mass, momentum, and energy exchange through the stream-halo interface are relative unknowns critical for determining the origin and fate of the Stream. Complementary to large-scale simulations of LMC-SMC interactions, we apply new insights derived from idealized, high-resolution cloud-crushing and radiative turbulent mixing layer simulations to the Leading Arm and Trailing Stream. Contrary to classical expectations of fast cloud breakup, we predict that the Leading Arm and much of the Trailing Stream should be surviving infall and even gaining mass due to strong radiative cooling. Provided a sufficiently supersonic tidal swing-out from the Clouds, the present-day Leading Arm could be a series of high-density clumps in the cooling tail behind the progenitor cloud. We back up our analytic framework with a suite of converged wind-tunnel simulations, finding that previous results on cloud survival and mass growth can be extended to high Mach number () flows with a modified drag time and longer growth time. We also simulate the Trailing Stream; we find that the growth time is long (approximately gigayears) compared to the infall time, and approximate Hα emission is low on average (∼ a few milliRayleigh) but can be up to tens of milliRayleigh in bright spots. Our findings also have broader extragalactic implications, e.g., galactic winds, which we discuss.

Distant probes of rotation measure structure: where is the Faraday rotation towards the Magellanic Leading Arm?

ABSTRACT Faraday rotation measures (RMs) should be interpreted with caution because there could be multiple magneto-ionized medium components that contribute to the net Faraday rotation along sightlines. We introduce a simple test using Galactic diffuse polarized emission that evaluates whether structures evident in RM observations are associated with distant circumgalactic medium or foreground interstellar medium. We focus on the Magellanic Leading Arm region where a clear excess of RM was previously reported. There are two gaseous objects standing out in this direction: the distant Magellanic Leading Arm and the nearby Antlia supernova remnant (SNR). We recognized narrow depolarized filaments in the $2.3\, \rm GHz$ S-band Polarization All Sky Survey image that overlaps with the reported RM excess. We suggest that there is a steep gradient in Faraday rotation in a foreground screen arising from the Antlia SNR. The estimated strength of the line-of-sight component of the magnetic field is $B_{\parallel }\sim 5\, \rm \mu G$, assuming that the excess of RM is entirely an outcome of the magnetized supernova shell. Our analysis indicates that the overlap between the RM excess and the Magellanic Leading Arm is only a remarkable coincidence. We suggest for future RM grid studies that checking Galactic diffuse polarization maps is a convenient way to identify local Faraday screens.

The Magellanic Corona as the key to the formation of the Magellanic Stream.

The dominant gaseous structure in the Galactic halo is the Magellanic Stream. This extended network of neutral and ionized filaments surrounds the Large Magellanic Cloud (LMC) and the Small Magellanic Cloud (SMC), the two most massive satellite galaxies of the Milky Way1-4. Recent observations indicate that the LMC and SMC are on their first passage around the Galaxy5, that the Magellanic Stream is made up of gas stripped from both clouds2,6,7 and that the majority of this gas is ionized8,9. Although it has long been suspected that tidal forces10,11 and ram-pressure stripping12,13 contributed to the formation of the Magellanic Stream, models have not been able to provide a full understanding of its origins3. Several recent developments-including the discovery of dwarf galaxies associated with the Magellanic group14-16, determination of the high mass of the LMC17, detection of highly ionized gas near stars in the LMC18,19 and predictions of cosmological simulations20,21-support the existence of a halo of warm (roughly 500,000 kelvin) ionized gas around the LMC (the 'Magellanic Corona'). Here we report that, by including this Magellanic Corona in hydrodynamic simulations of the Magellanic Clouds falling onto the Milky Way, we can reproduce the Magellanic Stream and its leading arm. Our simulations explain the filamentary structure, spatial extent, radial-velocity gradient and total ionized-gas mass of the Magellanic Stream. We predict that the Magellanic Corona will be unambiguously observable via high-ionization absorption lines in the ultraviolet spectra of background quasars lying near the LMC.

The Use Of Ultrasound Imaging In Assessing Ucl Width For Asymptomatic College Female Golfers

Injuries of the ulnar collateral ligament (UCL) are a common pathology and have been well documented for overhead athletes. However, little research has focused on the UCL in competitive golfers, though medial elbow injuries are commonly reported. Ultrasound imaging is becoming a common diagnostic tool to diagnose UCL pathology. In addition, USI protocols have been able to reliably identify the width of the UCL at the mid substance and apex of the trochlea. PURPOSE: The purpose of this pilot study was to examine if differences in UCL width at the mid substance and apex of trochlea existed between the trailing and lead arm of asymptomatic golfers. METHODS: Seven asymptomatic NCAA Division I collegiate female golfers (age 19.4 ± 1.4 yrs) participated in this study. Ultrasound images were obtained of the UCL on the participant’s trailing and lead arms using a GE LOGIQ e ultrasound unit. Participants were placed supine with elbow position at 30 degrees, with a wedge placed underneath the humerus creating a gravity induced valgus force on the UCL. Ultrasound imaging measurements to evaluate the UCL at two points were performed from the apex of the trochlea to the apex of the ulna. A paired t-test was performed to evaluate differences in UCL width measurements. RESULTS: The mean width of the UCL at mid-substance was .297cm (SD .047) in the trailing arm (mean width .297cm, SD .047) and . 234cm (SD .033) in the lead arm. The mean width at the apex of the trochlea was .129cm (SD .022) and .114cm (SD .016), respectively. There was a significant difference between the UCL measurements at the mid-substance between the leading and the trailing arm (mean difference .062 cm, t =5.680-, p= .036), but the difference at the trochlea was not statistically significant. CONCLUSIONS: The results of this pilot study indicate that the trailing arm’s UCL has a larger width when comparing trailing arm with leading arm, potentially due to the increased valgus forces on the trailing elbow versus the leading elbow. These changes may be associated with structural adaptions or pathologies related to this increased load. However, it was beyond the scope of this study to identify the potential sources of the differences in width of the UCL at the mid-substance. Further research is thus recommended using larger sample size, extended study period and symptomatic populations.

The Large Magellanic Cloud stellar content with SMASH

The Large Magellanic Cloud (LMC) is the closest and most studied example of an irregular galaxy. Among its principal defining morphological features, its off-centred bar and single spiral arm stand out, defining a whole family of galaxies known as the Magellanic spirals (Sm). These structures are thought to be triggered by tidal interactions and possibly maintained via gas accretion. However, it is still unknown whether they are long-lived stable structures. In this work, by combining photometry that reaches down to the oldest main sequence turn-off in the colour-magnitude diagrams (CMD, up to a distance of ∼4.4 kpc from the LMC centre) from the SMASH survey and CMD fitting techniques, we find compelling evidence supporting the long-term stability of the LMC spiral arm, dating the origin of this structure to more than 2 Gyr ago. The evidence suggests that the close encounter between the LMC and the Small Magellanic Cloud (SMC) that produced the gaseous Magellanic Stream and its Leading Arm also triggered the formation of the LMC’s spiral arm. Given the mass difference between the Clouds and the notable consequences of this interaction, we can speculate that this should have been one of their closest encounters. These results set important constraints on the timing of LMC-SMC collisions, as well as on the physics behind star formation induced by tidal encounters.

Kinematics of the Magellanic Stream and Implications for Its Ionization*

Abstract The Magellanic Stream and the Leading Arm form a massive, filamentary system of gas clouds surrounding the Large and Small Magellanic Clouds. Here we present a new component-level analysis of their ultraviolet (UV) kinematic properties using a sample of 31 sightlines through the Magellanic System observed with the Hubble Space Telescope/Cosmic Origins Spectrograph. Using Voigt-profile fits to UV metal-line absorption, we quantify the kinematic differences between the low-ion ( and ), intermediate-ion ( ), and high-ion ( and ) absorption lines and compare the kinematics between the Stream and Leading Arm. We find that the Stream shows generally simple, single-phase kinematics, with statistically indistinguishable b-value distributions for the low-, intermediate-, and high-ion components, all dominated by narrow ( km s−1) components that are well aligned in velocity. In contrast, we find tentative evidence that the Leading Arm shows complex, multi-phase kinematics, with broader high ions than low ions. These results suggest that the Stream is photoionized up to by a hard ionizing radiation field. This can be naturally explained by the Seyfert-flare model of Bland-Hawthorn, in which a burst of ionizing radiation from the Galactic Center photoionized the Stream as it passed below the south Galactic pole. The Seyfert flare is the only known source of radiation that is both powerful enough to explain the Hα intensity of the Stream and hard enough to photoionize and to the observed levels. The flare’s timescale of a few Myr suggests it is the same event that created the giant X-ray/γ-ray Fermi Bubbles at the Galactic Center.

Hα Distances to the Leading Arm of the Magellanic Stream

Abstract The Leading Arm (LA) is a tidal feature that is in front of the Magellanic Clouds (MCs) on their orbit through the Galaxy’s halo. Many physical properties of the LA, such as its mass and size, are poorly constrained because it has few distance measurements. While Hα measurements have been used to estimate the distances to halo clouds, many studies have been unsuccessful in detecting Hα from the LA. In this study, we explore a group of H i clouds which lie 75°–90° from the MCs. Through ultraviolet and 21 cm radio spectroscopy, this region, dubbed the LA Extension, was found to have chemical and kinematic similarities to the LA. Using the Wisconsin Hα Mapper, we detect Hα emission in four out of seven of our targets. Assuming that this region is predominantly photoionized, we use a radiation model that incorporates the contributions of the Galaxy, MCs, and the extragalactic background at z = 0 to derive a heliocentric distance of d ⊙ ≥ 13.4 . We also use this model to rederive Hα distances of d ⊙ ≥ 5.0 kpc and d ⊙ ≥ 22.9 to two clouds in the literature that might also be associated with the LA. Using these new measurements, and others in the literature, we provide a general trend of the variation of LA heliocentric distance as a function of Magellanic Stream longitude, and explore its implications for the origin and closest point of approach of the LA.

Spectroscopy of the Young Stellar Association Price-Whelan 1: Origin in the Magellanic Leading Arm and Constraints on the Milky Way Hot Halo

Abstract We report spectroscopic measurements of stars in the recently discovered young stellar association Price-Whelan 1 (PW 1), which was found in the vicinity of the Leading Arm (LA) of the Magellanic Stream (MS). We obtained Magellan+MIKE high-resolution spectra of the 28 brightest stars in PW 1 and used The Cannon to determine their stellar parameters. We find that the mean metallicity of PW 1 is [Fe/H] = −1.23 with a small scatter of 0.06 dex and the mean RV is = 276.7 with a dispersion of . Our results are consistent in , , and [Fe/H] with the young and metal-poor characteristics (116 Myr and [Fe/H] = −1.1) determined for PW 1 from our discovery paper. We find a strong correlation between the spatial pattern of the PW 1 stars and the LA II gas with an offset of −10.°15 in and +1.°55 in . The similarity in metallicity, velocity, and spatial patterns indicates that PW 1 likely originated in LA II. We find that the spatial and kinematic separation between LA II and PW 1 can be explained by ram pressure from Milky Way (MW) gas. Using orbit integrations that account for the LMC and MW halo and outer disk gas, we constrain the halo gas density at the orbital pericenter of PW 1 to be and the disk gas density at the midplane at to be . We, therefore, conclude that PW 1 formed from the LA II of the MS, making it a powerful constraint on the MW–Magellanic interaction.

Discovery of a Disrupting Open Cluster Far into the Milky Way Halo: A Recent Star Formation Event in the Leading Arm of the Magellanic Stream?

Abstract We report the discovery of a young ( ), low-mass ( ), metal-poor ( ) stellar association at a heliocentric distance , placing it far into the Milky Way (MW) halo. At its present Galactocentric position , the association is (on the sky) near the leading arm of the gas stream emanating from the Magellanic Cloud system, but is located from the Large Magellanic Cloud center on the other side of the MW disk. If the cluster is colocated with H i gas in the stream, we directly measure the distance to the leading arm of the Magellanic stream. The measured distance is inconsistent with Magellanic stream model predictions that do not account for ram pressure and gas interaction with the MW disk. The estimated age of the cluster is consistent with the time of last passage of the leading arm gas through the Galactic midplane; we therefore speculate that this star formation event was triggered by its last disk midplane passage. Most details of this idea remain a puzzle: the Magellanic stream has low column density, the MW disk at large radii has low gas density, and the relative velocity of the leading arm and MW gas is large. However it formed, the discovery of a young stellar cluster in the MW halo presents an interesting opportunity for study. This cluster was discovered with Gaia astrometry and photometry alone, but follow-up DECam photometry was crucial for measuring its properties.

Young stars raining through the galactic halo: the nature and orbit of price-whelan 1

ABSTRACT We present radial velocities for five member stars of the recently discovered young (age ≃ 100−150 Myr) stellar system Price-Whelan 1 (PW 1), which is located far away in the Galactic Halo (D≃ 29 kpc, Z≃ 15 kpc), and that is probably associated with the leading arm (LA) of the Magellanic Stream. We measure the systemic radial velocity of PW 1, Vr = 275 ± 10 km s−1, significantly larger than the velocity of the LA gas in the same direction. We re-discuss the main properties and the origin of this system in the light of these new observations, computing the orbit of the system and comparing its velocity with that of the H i in its surroundings. We show that the bulk of the gas at the velocity of the stars is more than 10 deg (5 kpc) away from PW 1 and the velocity difference between the gas and the stars becomes larger as gas closer to the stars is considered. We discuss the possibilities that (1) the parent gas cloud was dissolved by the interaction with the Galactic gas, and (2) that the parent cloud is the high-velocity cloud (HVC) 287.5+22.5 + 240, lagging behind the stellar system by ≃ 25 km s−1 and ≃10 deg ≃ 5 kpc. This HVC, which is part of the LA, has metallicity similar to PW 1, displays a strong magnetic field that should help to stabilize the cloud against ram pressure, and shows traces of molecular hydrogen. We also show that the system is constituted of three distinct pieces that do not differ only by position in the sky but also by stellar content.

The Magellanic System: the puzzle of the leading gas stream

ABSTRACT The Magellanic Clouds (MCs) are the most massive gas-bearing systems falling into the Galaxy at the present epoch. They show clear signs of interaction, manifested in particular by the Magellanic Stream, a spectacular gaseous wake that trails from the MCs extending more than 150° across the sky. Ahead of the MCs is the ‘Leading Arm’ usually interpreted as the tidal counterpart of the Magellanic Stream, an assumption we now call into question. We revisit the formation of these gaseous structures in a first-infall scenario, including for the first time a Galactic model with a weakly magnetized, spinning hot corona. In agreement with previous studies, we recover the location and the extension of the Stream on the sky. In contrast, we find that the formation of the Leading Arm – that is otherwise present in models without a corona – is inhibited by the hydrodynamic interaction with the hot component. These results hold with or without coronal rotation or a weak, ambient magnetic field. Since the existence of the hot corona is well established, we are led to two possible interpretations: (i) the Leading Arm survives because the coronal density beyond 20 kpc is a factor ≳10 lower than required by conventional spheroidal coronal X-ray models, in line with recent claims of rapid coronal rotation; or (ii) the ‘Leading Arm’ is cool gas trailing from a frontrunner, a satellite moving ahead of the MCs, consistent with its higher metallicity compared to the trailing stream. Both scenarios raise issues that we discuss.

Towards a complete understanding of the Magellanic Stream Formation

The Magellanic Clouds (MCs) have lost most of their gas during their passage by the Milky Way, a property that has never been successfully modelled. Here, we use accurate and mesh-free hydrodynamic simulations to reproduce the Magellanic Stream and the MCs in the frame of a ‘ram-pressure plus collision’ model. This model reproduces many of the observed properties of the H i Stream including most of its density profile along its length and its dual filamentary structure. Besides this, ram-pressure combined with Kelvin–Helmholtz instabilities extracts amounts of ionized and H i gas consistent with those observed. The modelled scenario also reproduces the Magellanic Bridge, including the offset between young and old stars, and the collision between the Clouds, which is responsible of the very elongated morphology of the Small Magellanic Cloud along the line of sight. This model has solved most of the mysteries linked to the formation of the Magellanic Stream. The Leading Arm is not reproduced in the current model because it requires an alternative origin.

The Origins of Young Stars in the Direction of the Leading Arm of the Magellanic Stream: Abundances, Kinematics, and Orbits.

We explore the origins of the young B-type stars found by Casetti-Dinescu et al. (2014) at the outskirts of the Milky-Way disk in the sky region of Leading Arm of the Magellanic Stream. High-resolution spectroscopic observations made with the MIKE instrument on the Magellan Clay 6.5m telescope for nine stars are added to the previous sample analyzed by Zhang et al. (2017). We compile a sample of fifteen young stars with well-determined stellar types, ages, abundances and kinematics. With proper motions from Gaia DR2 we also derive orbits in a realistic Milky-Way potential. We find that our previous radial-velocity selected LA candidates have substantial orbital angular momentum. The substantial amount of rotational component for these stars is in contrast with the near-polar Magellanic orbit, thus rendering these stars unlikely members of the LA. There are four large orbital-energy stars in our sample. The highest orbital-energy one has an age shorter than the time to disk crossing, with a birthplace z = 2.5 kpc and R GC ~ 28 kpc. Therefore, the origin of this star is uncertain. The remaining three stars have disk runaway origin with birthplaces between 12 and 25 kpc from the Galactic center. Also, the most energetic stars are more metal poor ([Mg/H] =-0.50 ± 0.07) and with larger He scatter (σ [He/H] = 0.72) than the inner disk ones ([Mg/H] = 0.12 ± 0.36, σ [He/H] = 0.15). While the former group's abundance is compatible with that of the Large Magellanic Cloud, it could also reflect the metallicity gradient of the MW disk and their runaway status via different runaway mechanisms.

New Constraints on the Nature and Origin of the Leading Arm of the Magellanic Stream

Abstract We present a new precision measurement of gas-phase abundances of S, O, N, Si, Fe, P, Al, Ca as well as molecular hydrogen (H2) in the Leading Arm (region II, LA II) of the Magellanic Stream (MS) toward the Seyfert galaxy NGC 3783. The results are based on high-quality archival ultraviolet/optical/radio data from various different instruments (HST/STIS, FUSE, AAT, GBT, GB140 ft, ATCA). Our study updates previous results from lower-resolution data and provides for the first time a self-consistent component model of the complex multiphase absorber, delivering important constraints on the nature and origin of LA II. We derive a uniform, moderate α abundance in the two main absorber groups at +245 and +190 km s−1 of α/H = 0.30 ± 0.05 solar, a low nitrogen abundance of N/H = 0.05 ± 0.01 solar, and a high dust content with substantial dust depletion values for Si, Fe, Al, and Ca. These α, N, and dust abundances in LA II are similar to those observed in the Small Magellanic Cloud (SMC). From the analysis of the H2 absorption, we determine a high thermal pressure of P/k ≈ 1680 K cm−3 in LA II, in line with the idea that LA II is located in the inner Milky Way halo at a z-height of &lt;20 kpc, where it hydrodynamically interacts with the ambient hot coronal gas. Our study supports a scenario in which LA II stems from the breakup of a metal- and dust-enriched progenitor cloud that was recently (200–500 Myr ago) stripped from the SMC.

The Fate of Supernova-heated Gas in Star-forming Regions of the LMC: Lessons for Galaxy Formation?

Abstract Galactic winds and fountains driven by supernova-heated gas play an integral role in redistributing gas in galaxies, depositing metals in the circumgalactic medium, and quenching star formation. The interplay between these outflows and ram-pressure stripping (RPS) due to the galaxy’s motion through an ambient medium may enhance these effects by converting fountain flows into expelled gas. In this paper, we present controlled, 3D simulations of RPS combined with thermally driven, local outflows from clustered supernovae in an isolated disk galaxy modeled on the Large Magellanic Cloud (LMC), a dwarf satellite of the Milky Way on its first infall. Observational evidence of local outflows emanating from supergiant shells in the LMC and a trailing filament of H i gas originating from these regions—with no obvious Leading Arm counterpart—may represent a perfect example of this process. Our simulations present a proof of concept that ram pressure can convert fountain flows into expelled gas. We find that fountains launched near the peak star formation time of the LMC can comprise part of the LMC filament in the Trailing Stream but with lower column densities than observed. Larger, more numerous outflows from the LMC may be possible and may contribute more mass, but higher-inertia gas will lengthen the timescale for this gas to be swept away by ram pressure. Given the high-resolution observations, increased knowledge of star formation histories, and growing evidence of multiphase ionized outflows, the LMC is an ideal test bed for future wind models.

ATP Binding to Rad5 Initiates Replication Fork Reversal by Inducing the Unwinding of the Leading Arm and the Formation of the Holliday Junction

Replication fork reversal is one of the major pathways for reactivating stalled DNA replication. Many enzymes with replication fork reversal activity have DNA-unwinding activity as well, but none of the fork reversal enzymes in the SWI/SNF family shows a separate DNA-unwinding activity, raising the question of how they initiate the remodeling process. Here, we found ATP binding to Rad5 induces the unwinding of the leading arm of the replication fork and proximally positions the leading and lagging arms. This facilitates the spontaneous remodeling of thereplication fork into a four-way junction. Once the four-way junction is formed, Rad5 migrates the four-way junction at a speed of 7.1 ± 0.14 nt/s. The 3' end anchoring of the leading arm by Rad5's HIRAN domain is critical for both branch migration and the recovery of the three-way junction, but not for the structural transition to the four-way junction.

Models of Tidally Induced Gas Filaments in the Magellanic Stream

Abstract The Magellanic Stream and Leading Arm of H i that stretches from the Large and Small Magellanic Clouds (LMC and SMC) and over 200° of the Southern sky is thought to be formed from multiple encounters between the LMC and SMC. In this scenario, most of the gas in the Stream and Leading Arm is stripped from the SMC, yet recent observations have shown a bifurcation of the Trailing Arm that reveals LMC origins for some of the gas. Absorption measurements in the Stream also reveal an order of magnitude more gas than in current tidal models. We present hydrodynamical simulations of the multiple encounters between the LMC and SMC at their first pass around the Milky Way, assuming that the Clouds were more extended and gas-rich in the past. Our models create filamentary structures of gas in the Trailing Stream from both the LMC and SMC. While the SMC trailing filament matches the observed Stream location, the LMC filament is offset. In addition, the total observed mass of the Stream in these models is underestimated by a factor of four when the ionized component is accounted for. Our results suggest that there should also be gas stripped from both the LMC and SMC in the Leading Arm, mirroring the bifurcation in the Trailing Stream. This prediction is consistent with recent measurements of spatial variation in chemical abundances in the Leading Arm, which show that gas from multiple sources is present, although its nature is still uncertain.