As reverse electrodialysis (RED) technology evolves, achieving high-performance osmotic energy conversion (OEC) necessitates porous membranes with exceptional ion selectivity and permeability. Two-dimensional membranes crafted from graphene oxide (GO) or MXene stand out for their immensely high surface charges, which facilitate superb ion selectivity during transport. Their unique layered stacking structure further yields ultra-small nanochannel radii, bolstering ion selectivity while maintaining high permeability. This makes them prime candidates for osmotic energy conversion. However, the presence of divalent ions in natural seawater readily alters the surface charge density and interlayer spacing of these 2D membranes, significantly impacting their OEC performance, resulting in a power density reduction of about 6 % -10 %. Prior research focused on the ionic properties of divalent ions but overlooked their impact on the nanochannel structure, including changes in interlayer spacing and surface charge density. This study thus delves into the OEC performance variations of GO and MXene 2D layered membranes under varying divalent ion concentrations and salt ratios. Starting from the ion properties of divalent ions and their impact on the nanochannel structure, combined with simulation research, explain their mechanism of action. This research offers theoretical foundations for the future design and application of 2D layered membranes, providing novel solutions and insights to tackle critical challenges like enhancing power density and adapting to real seawater conditions.