Lavandula Essential Oils Research Articles

Effect of collagen hydrogel containing Lavandula officinalis essential oil nanoemulsion in wound healing of infectious burn.

The main cause of mortality in burn patients is infection from burns. Drug-resistant bacteria are the main causes of wound infection, so alternative antibiotic therapies hold significant importance. The objective of this study was to examine the impact of a collagen hydrogel that contains a nanoemulsion of Lavandula essential oil on the healing process of infected burn wounds. In this experimental study, 20 rats were randomly divided after applying burns with a 10 mm diameter hot plate and infecting the wounds with multidrug-resistant Pseudomonas aeruginosa into four groups, including a positive control, a negative control, the first experiment (collagen hydrogel), and the second experiment (collagen hydrogel containing Lavandula essential oil nanoemulsion). On the 4th, 11th, and 18th days, tissue samples were taken for pathology studies. The important parameters in burn wound healing with hematoxylin and eosin and Masson's trichrome staining methods were investigated and scored according to Abramov's method. Based on the pathology findings, experimental groups 1 and 2 compared to the negative and positive control groups were effective in rat infection wound healing. The hydrogel scaffold in the experimental groups increased fibroblasts and angiogenesis compared to the control groups. Epithelization was noticed only in the hydrogel group containing nanoemulsion. The study findings suggest that the use of collagen hydrogel with Lavandula essential oil nanoemulsion has potential as a wound dressing. This is because it has the potential to effectively promote healing and act as an antibacterial agent to prevent infections.

Chemical composition, antioxidant and antimicrobial effect of Lavandula essential oil used as a natural antioxidant for cold-pressed oils

Chemical composition, antioxidant and antimicrobial effect of Lavandula essential oil used as a natural antioxidant for cold-pressed oils

The effects of lavandula aromatherapy on pain relief after coronary artery bypass graft surgery: A randomized clinical trial

Background and aimsCoronary artery bypass graft surgery (CABG) is a primary treatment in coronary artery disease (CAD). Patients experience pain after CABG. Pain may increase postoperative complications and decrease quality of life (QoL). This study aims to determine the lavandula aromatherapy effect on patients' pain after CABG. Methods98 patients undergoing CABG were randomly assigned to intervention and placebo groups. The intervention group inhaled 5-drops of 20 % lavandula essential oil (LEO) and the placebo group was exposed to 5-drop of distilled water. LEO or distilled water were dropped into a sterile gauze converted to the form of a necklace. The subjects wore necklace for three consecutive days and intervention was performed each 24 h. The pain intensity, systolic blood pressure (SBP), and diastolic blood pressure (DBP) were measured before each intervention and 30 min after it. Also, the mean analgesic intake was recorded. SPSS version 16 was used to analyze the data. ResultsThe mean pain score measured in the intervention group showed a more decrease than that obtained in the placebo group after the first and second interventions. Additionally, the mean pain score in the intervention group demonstrated a significant decrease than the placebo group after the second intervention (P=0.008). Moreover, mean SBP after the second intervention (P=0.046) and mean DBP after the first intervention (P=0.029) revealed a significant difference between two groups. Moreover, the intervention group received less analgesic than the placebo group. ConclusionsLavandula aromatherapy can reduce pain and received analgesic dose after CABG.

Essential Oil Content and Compositional Variability of Lavandula Species Cultivated in the Mid Hill Conditions of the Western Himalaya.

The increase in the utilization of Lavandula essential oil in industries led to an impressive rise in the demand for quality essential oils. However, a post-harvest drying of Lavandula species can be a decisive factor to determine the quantity and quality of essential oil. The study was conducted in western Himalayan conditions to assess the essential oil content and composition of two Lavandula species viz., lavender (Lavandula angustifolia Mill.), and lavandin (Lavandula × intermedia Emeric ex Loisel), at four different drying duration (0 h, 24 h, 48 h and 72 h after the harvest). The higher growth attributes viz., plant height (71.7 cm), ear length (8.8 cm), number of spikes (18.1), and number of flowers per ear (47.5) were higher in lavandin, while the number of branches (17.1) was higher in lavender. Essential oil content (%) and moisture reduction (%) were significantly higher at 72 h than at 0 h. The major components of lavender and lavandin essential oil were linalool (33.6–40.5%), linalyl acetate (10.8–13.6%), lavandulyl acetate (2.8–14.5%), and linalyl propionate (5.3–14.1%) in both the Lavandula species. There was a decreasing trend in linalool and an increasing trend in linalyl acetate content in lavandin, with an increase in drying duration up to 72 h; while in lavender, no regular trend was observed in linalool and linalyl acetate content. It was observed that linalool and linalyl acetate levels were the highest at 24 and 0 h of drying in lavender and lavandin, respectively, and essential oil extraction can be done according to the desire of the constituent at varied drying duration.

Antibiofilm Potential of Lavandula Preparations against Campylobacter jejuni.

New approaches for the control of Campylobacter jejuni biofilms in the food industry are being studied intensively. Natural products are promising alternative antimicrobial substances to control biofilm production, with particular emphasis on plant extracts. Dried flowers of Lavandula angustifolia were used to produce essential oil (LEO), an ethanol extract (LEF), and an ethanol extract of Lavandula postdistillation waste material (LEW). The chemical compositions determined for these Lavandula preparations included seven major compounds that were selected for further testing. These were tested against C. jejuni for biofilm degradation and removal. Next-generation sequencing was used to study the molecular mechanisms underlying LEO actions against C. jejuni adhesion and motility. Analysis of LEO revealed 1,8-cineol, linalool, and linalyl acetate as the main components. For LEF and LEW, the main components were phenolic acid glycosides, with flavonoids rarely present. The MICs of the Lavandula preparations and pure compounds against C. jejuni ranged from 0.2 mg/ml to 1 mg/ml. LEO showed the strongest biofilm degradation. The reduction of C. jejuni adhesion was ≥1 log10 CFU/ml, which satisfies European Food Safety Authority recommendations. Lavandula preparations reduced C. jejuni motility by almost 50%, which consequently can impact biofilm formation. These data are in line with the transcriptome analysis of C. jejuni, which indicated that LEO downregulated genes important for biofilm formation. LEW also showed good antibacterial and antibiofilm effects, particularly against adhesion and motility mechanisms. This defines an innovative approach using alternative strategies and novel targets to combat bacterial biofilm formation and, hence, the potential to develop new effective agents with biofilm-degrading activities. IMPORTANCE The Lavandula preparations used in this study are found to be effective against C. jejuni, a common foodborne pathogen. They show antibiofilm properties at subinhibitory concentrations in terms of promoting biofilm degradation and inhibiting cell adhesion and motility, which are involved in the initial steps of biofilm formation. These results are confirmed by transcriptome analysis, which highlights the effect of Lavandula essential oil on C. jejuni biofilm properties. We show that the waste material from the hydrodistillation of Lavandula has particular antibiofilm effects, suggesting that it has potential for reuse for industrial purposes. This study highlights the need for efforts directed toward such innovative approaches and alternative strategies against biofilm formation and maintenance by developing new naturally derived agents with antibiofilm activities.

THE EFFECT OF COMPLEMENTARY THERAPIES OF SLOW DEEP BREATHING AND LAVANDULA ESSENTIAL OIL FOR INCREASING FEMALE PRISONERS’ SELF-ESTEEM AT THE CORRECTIONAL FACILITY, CLASS IIB, YOGYAKARTA

Prisoners will undoubtedly experience major changes in the prison in terms of environment, roles, and activities during their detention. All these changes make them experience a decrease in self-esteem. Complementary therapy in the forms of slow deep breathing and essential oil of Lavandula can cause physical and psychological relaxation so that it is expected to affect the prisoners’ self-esteem. This study aimed to find out the effect of complementary therapies of slow deep breathing and Lavandula essential oil on female prisoners’ self-esteem. The type of this study was experimental research using a pre-post-test design and a two-group design. Samples in this study consisted of 41 respondents. Furthermore, the employed data analysis was the Wilcoxon test. The results in this study indicated that the majority of respondents were in the categories of early adulthood (65.9%), having secondary education level (48.8%), having been married (48.8%), and having a length of sentence of 1 – 5 years (61%). In the group given slow deep breathing therapy, respondents who had low self-esteem in the pre-test was 33.33%. However, in the post-test, they decreased to 5.56%. Meanwhile, its significance value (2-tailed) was 0.025 (p < 0.05). In the group given Lavandula essential oil therapy, respondents who had low self-esteem in the pre-test was 34.78%. However, in the post-test, they also decreased to 4.34%. Meanwhile, its significance value (2-tailed) was 0.008 (p < 0.05). It can be concluded that complementary therapies of slow deep breathing and Lavandula essential oil can increase female prisoners’ self-esteem.

Antifungal Activity and Chemical Composition of Seven Essential Oils to Control the Main Seedborne Fungi of Cucurbits.

Essential oils represent novel alternatives to application of synthetic fungicides to control against seedborne pathogens. This study investigated seven essential oils for in vitro growth inhibition of the main seedborne pathogens of cucurbits. Cymbopogon citratus essential oil completely inhibited mycelial growth of Stagonosporopsis cucurbitacearum and Alternaria alternata at 0.6 and 0.9 mg/mL, respectively. At 1 mg/mL, Lavandula dentata, Lavandula hybrida, Melaleuca alternifolia, Laurus nobilis, and two Origanum majorana essential oils inhibited mycelia growth of A. alternata by 54%, 71%, 68%, 36%, 90%, and 74%, respectively. S. cucurbitacearum mycelia growth was more sensitive to Lavandula essential oils, with inhibition of ~74% at 1 mg/mL. To determine the main compounds in these essential oils that might be responsible for this antifungal activity, they were analyzed by gas chromatography–mass spectrometry (GC-MS). C. citratus essential oil showed cirtal as its main constituent, while L. dentata and L. nobilis essential oils showed eucalyptol. The M. alternifolia and two O. majorana essential oils had terpinen-4-ol as the major constituent, while for L. hybrida essential oil, this was linalool. Thus, in vitro, these essential oils can inhibit the main seedborne fungi of cucurbits, with future in vivo studies now needed to confirm these activities.

Essential oils of spontaneous species of the genus Lavandula from Portugal: a brief review.

Spontaneous lavender growing in uncultivated fields in Portugal have been used in traditional medicine for internal and external uses. The essential oils (EOs) of Lavandula stoechas subsp. luisieri are characterized by the presence of trans-α-necrodyl acetate and trans-necrodol. These EOs are able to prevent the generation and deposition of neurotoxic β-amyloid peptide in Alzheimer's disease. The EOs also present antibacterial, anti-fungal, anti-Leishmania, antioxidant, anti-inflammatory and antifeedant effects. In the case of hydrodistillation, the predominant compound of Lavandula viridis EO was 1,8-cineole, nevertheless in the case of supercritical fluid extraction, the main constituent was camphor. In in vitro shoots EOs, 1,8-cineole and α-pinene were the most important compounds. The EOs presented anti-fungal activity particularly against Cryptococcus neoformans and dermatophytes. The antioxidant and anti-protozoal activities of L. viridis EOs were lower than L. stoechas subsp. luisieri EOs, with hydrodistillation being the best method for obtaining samples with higher antioxidant and anti-acetylcholinesterase activities. The presence of fenchone, 1,8-cineole and camphor was a common trace of the Lavandula pedunculata subsp. pedunculata EOs and in in vitro axillary shoots EOs. Lavandula pedunculata subsp. lusitanica EOs were predominantly constituted of fenchone and camphor. The antioxidant activity of L. pedunculata subsp. lusitanica EOs was poorer than other Lavandula EOs from Portugal.

Ferulic Acid-NLC with Lavandula Essential Oil: A Possible Strategy for Wound-Healing?

Nowadays, an increasing interest in combinatorial drug delivery systems is emerging, highlighting the possibility of exploiting essential oils (EO) for topical applications. This work aimed at developing nanostructured lipid carriers (NLC) for the combined delivery of ferulic acid and Lavandula EO, whose beneficial effects in wound-healing processes have been widely reported. Homogeneous (polydispersity index, PDI < 0.2) nanoparticles with a small size (<150 nm) and a high encapsulation efficiency (>85%) were obtained. The co-presence of ferulic acid and Lavandula EO, as compared to synthetic isopropyl myristate-based NLC, increased nanoparticles’ stability, due to higher ordering chains, as confirmed by morphological and physicochemical studies. An enhanced cytocompatibility was observed when combining ferulic acid and Lavandula EO, as confirmed by in vitro studies on fibroblasts. Furthermore, the combined delivery of ferulic acid and Lavandula EO significantly promoted cell migration with higher effectiveness in respect to the free drug solution and the carrier without the EO. Taken all together, our results suggest a potential combined effect of the antioxidant ferulic acid and Lavandula EO co-delivered in lipid nanoparticles in promoting cell proliferation and migration, representing a promising strategy in the treatment of wounds.

Invitro Determination of Chemical Compounds and Antibacterial Activity of Lavandula Essential oil against some Pathogenic Microorganisms

Invitro Determination of Chemical Compounds and Antibacterial Activity of Lavandula Essential oil against some Pathogenic Microorganisms

A comparative study of terpene composition in different clades of the genus Lavandula

ABSTRACTThe genus Lavandula L. (Lamiaceae) comprises 40 species, of which only a few are used to extract essential oils for perfume and detergent industries. To date, the compound diversity of Lavandula essential oils, and most notably terpene variation amongst species, is poorly documented. In this work, we report the diversity of terpene content in 33 accessions from the subgenera Lavandula and Fabricia. We used these terpenes to distinguish subgenera and sections and to find autapomorphic and synapomorphic traits as taxonomic markers. We demonstrate that terpene composition is not appropriate for phylogenetic reconstruction and chemotaxonomy in Lavandula, but we show nevertheless that terpene diversity allow for distinguishing the two subgenera, Lavandula and Fabricia and for separating the sections, respectively i/Dentatae, Stoechas and Lavandula, and ii/Pterostoechas, Chaetostachys, Subnudae and Hasikenses. Furthermore, amongst terpenes with putative ecological roles, we found that camphor, 1,8-cineole and carvacrol are synapomorphic traits, or at least markers respective of each subgenus, that trans-α-necrodol is an autapomorphic trait of L. stoechas subsp. luisieri and that linalool is either a symplesiomorphic trait or a homoplasic trait. Based on these results, we propose evolutionary hypotheses and future work directions relative to the putative ecological role of these terpenes in other species and to terpene biosynthesis studies in Lavandula.

Chemical composition, antimicrobial and antioxidant activities of essential oils of Lavandula × intermedia ‘Budrovka’ and L. angustifolia cultivated in Croatia

In the search for new natural resources with promising biological activity, chemical composition as well as antibacterial, antifungal and antioxidant activities of the essential oil from Croatian indigenous cultivar of lavandin (Lavandula × intermedia‘Budrovka’) were studied for the first time and compared with those of lavender (L. angustifolia) cultivated under the same pedo-climatic conditions of central Croatia. Forty compounds were identified in the studied Lavandula essential oils by GC–MS analysis. Linalool (57.1%), linalyl acetate (9.8%) and 1,8-cineole (8.4%) were the most abundant compounds of lavandin ‘Budrovka’ oil while lavender oil contained mainly linalool (54.0%), linalyl acetate (11.6%) and lavandulol (6.6%). Both essential oils were characterized by a very low amount of camphor (≤0.3%). The antimicrobial activities of two Lavandula essential oils and their dominant constituents were evaluated against 30 medically important pathogens and microbial contaminants of food, cosmetic and pharmaceutical products using agar well diffusion and broth dilution methods. L. × intermedia ‘Budrovka’ essential oil showed a broad-spectrum of antibacterial (MICs 0.25–2.5 mg/mL) and antifungal (MICs 0.1–2 mg/mL) activities, being generally more than or at least as effective as those from L. angustifolia. Among the two dominant compounds which were also tested comparatively and found to exhibit antimicrobial effect, linalool has been pointed out as much more potent agent then linalyl acetate. Antioxidant studies based on DPPH assay indicated that the lavandin ‘Budrovka’ essential oil has significantly stronger antioxidant properties than lavender essential oil (IC50 21.6 mg/mL and 27.7 mg/mL, respectively). This study highlighted Croatian indigenous cultivar lavandin ‘Budrovka’ as a rich source of essential oil with pronounced antimicrobial properties due to abundance of linalool and indicated its potential use as biopreservative in food, pharmaceutical and cosmetic products or as natural remedy with promising health benefit.

Classification of lavender essential oils: sedative effects of Lavandula oils

The effects of lavender volatile inhalation on human behaviour and emotions are not reproducible, because several types of lavender essential oils are available in markets. The aim of this study was to determine the key volatile components for the sedative effect of lavender essential oil on human. We tested twenty-seven Lavandula essential oils and analysed their volatile component compositions by means of GC and GC-MS. Volatile component composition of the essential oils was categorized by Principal Components Analysis and seven essential oils were selected for physiological/psychological evaluation. Power spectral analysis on R–R intervals, salivary amylase activity and POMS test showed that some essential oils showed sedative effects on humans. Oils with stimulant effects were also observed. Differences in volatile composition between sedative and stimulant oils suggested that key components of aromachology effects of Lavandula oils were linalyl acetate (sedative effect) and camphor (stimulant effect).

Etlik Piliçlerde Karma Yeme Farklı Esansiyel Yağlar ve Karışımlarının İlavesinin Performans ve Karkas Özellikleri Üzerine Etkisi

Bu çalışma, etlik piliçlerde kekik (Thymus vulgaris L.), biberiye (Rosmarinus officinalis L.) ve karabaş otu (Lavandula stoechas L.) esansiyel yağları ve bunların karışımlarının canlı ağırlık, canlı ağırlık artışı, yem tüketimi, yemden yararlanma oranı ve karkas özellikleri üzerine etkisini tespit etmek için yapılmıştır. Çalışmada, günlük yaşta toplam 640 adet etlik civciv (Ross 308) kullanılmış ve 5 tekerrürlü olacak şekilde 8 muamele grubuna tesadüfü olarak yerleştirilmiştir. Her bir muamele grubunda 80 adet civciv bulunmaktadır. Deneme rasyonları sırasıyla kontrol (0 mg/kg), ilave kekik esansiyel yağı (50 mg/kg), biberiye esansiyel yağı (50 mg/kg), karabaş otu esansiyel yağı (50 mg/kg), kekik + biberiye (25+25 mg/kg), kekik + karabaş otu (25+25 mg/kg), biberiye + karabaş otu (25+25 mg/kg) ve kekik+biberiye+karabaş otu (16,7 + 16,7 + 16,7 mg/kg) oluşmaktadır. Yem ve su ad libitum olarak sağlanmıştır. Çalışma 6 hafta sürmüştür. Performans parametreleri haftalık olarak belirlenmiştir. Denemenin 42. gününde hayvanlar kesilerek karkas özellikleri tespit edilmiştir. Çalışma sonuçlarına göre, rasyon farklı esansiyel yağ ve bunların karışımlarının etlik piliçlerde canlı ağırlık, canlı ağırlık artışı, yem tüketimi, yem değerlendirme oranı ve karkas özellikleri üzerine etkisi önemli olmamıştır.

The effect of lavandula essential oils on release of niflumic acid from collagen hydrolysates

The aim of this paper is to design and characterize some drug delivery systems (DDS) based on collagen hydrolysates (H), niflumic acid as a non-steroidal anti-inflammatory model drug and two essential oils of Lavandula officinalis L. (LO) and Lavandula stoechas L. subsp. Stoechas (LS), for use in healing cutaneous wounds with post-lesion inflammatory response. The essential oils are characterized by GC-MS. The wettability capacity of collagen hydrolysate powders was assessed by contact angle measurement. Niflumic acid release was investigated using a modified Franz diffusion cell, and the diffusion coefficient, time-lag and drug flux were determined. The kinetic parameters were found to be influenced by different concentrations and types of essential oils. These therapeutical products, based on collagen hydrolysates and synergistic drug associations, could have potential biomedical application in wound healing treatment.

Chemical composition of Rosmarinus and Lavandula essential oils and their insecticidal effects on Orgyia trigotephras (Lepidoptera, Lymantriidae)

To evaluate toxic activities of essential oils obtained from Rosmarinus officinalis and Lavandula stoechas against the fourth larval instars of Orgyia trigotephras. A total of 1 200 larvae were divided into three groups I II III. Group I was to investigate the effect of extracted essential oils from these aromatic plants as gastric disturbance. Bacillus thuringiensis and ethanol were used as control group. Group II was used as contact action and Group III was used as fumigant action. Decis and ethanol were used as control group. During the three experiments, the effect of essential oils on larvae was assessed. The chemical composition of essential oils from two medicinal plants was determined and, their insecticidal effects on the fourth larval state of Orgyia trigotephras were assessed. The two simples presented an insecticidal activity, nevertheless Rosmarinus officinalis essential oil was less efficient compared to Lavandula stoechas one are discussed. The relationship between the chemical composition and the biological activities is confirmed by the present findings. Therefore the potential uses of these essential oils as bioinsecticides can be considered as an alternative to the use of synthetic products.

Antiparasitic activity of two Lavandula essential oils against Giardia duodenalis, Trichomonas vaginalis and Hexamita inflata

Two essential oils derived from Lavandula angustifolia and Lavandula x intermedia were investigated for any antiparasitic activity against the human protozoal pathogens Giardia duodenalis and Trichomonas vaginalis and the fish pathogen Hexamita inflata: all of which have significant infection and economic impacts. The study has demonstrated that low (< or = 1%) concentrations of L. angustifolia and L. x intermedia oil can completely eliminate T. vaginalis, G. duodenalis and H. inflata in vitro. At 0.1% concentration, L. angustifolia oil was found to be slightly more effective than L x intermedia oil against G. duodenalis and H. inflata. The potential applications are discussed.