The lateral wind load applied to high-rise buildings results in movements that can cause creaking noises in drywall partitions with suspended ceiling systems. These noises can be disruptive to the residents and impair the structure’s serviceability. In this study, a sequence of large-scale three-dimensional (3D) experiments was conducted on two assemblies of drywall partitions with a suspended ceiling system to address this serviceability issue. The goal was to explore the 3D response of these assemblies, with a key emphasis on validating the effectiveness of adhesives in mitigating noise introduced by screw fixings, not only for individual walls but also when interacting with neighbouring walls and ceilings, which represent the real-world conditions. The six-degree of freedom (6-DOF) Multi-Axis Substructure Testing (MAST) system at Swinburne University Technology was used to simulate realistic wind load conditions. Two sets of test specimens were constructed using different methods to fix the plasterboard wall lining to the cold-formed steel (CFS) frame, which was rigidly bolted to both the MAST crosshead and the floor below. The first set of specimens (Assembly 1) had the plasterboards screw-fixed to the steel frame, while the second set (Assembly 2) used adhesive-only or a combination of adhesive and screw-fixing methods. Additionally, the instrumentation employed for acoustic measurements was strategically chosen to capture the noise generated by the overall system-level response. This served as a means of validating the serviceability noise experienced by users/occupants. Sound pressure level measurements and an acoustic camera were used simultaneously to identify the noise sources. The creaking noise measurements were compared for different specimens of Assemblies 1 and 2 and the effects of different construction details (e.g. fixing method, plasterboard ceiling lining, etc.) on wind-induced creaking noises were discussed. The results of this study can provide valuable insights for manufacturers and designers to minimize creaking noises in drywall partition systems and propose new construction techniques to improve the serviceability of high-rise buildings.