With the increasing level of industrial informatization, massive industrial data require real-time and high-fidelity wireless transmission. Although some industrial wireless network protocols have been designed over the last few decades, most of them have limited coverage and narrow bandwidth. They cannot always ensure the certainty of information transmission, making it especially difficult to meet the requirements of low latency in industrial manufacturing fields. The 5G technology is characterized by a high transmission rate and low latency; therefore, it has good prospects in industrial applications. To apply 5G technology to factory environments with low latency requirements for data transmission, in this study, we analyze the statistical performance of the round-trip time (RTT) in a 5G-R15 communication system. The results indicate that the average value of 5G RTT is about 11 ms, which is less than the 25 ms of WIA-FA. We then consider 5G RTT data as a group of time series, utilizing the augmented Dickey–Fuller (ADF) test method to analyze the stability of the RTT data. We conclude that the RTT data are non-stationary. Therefore, firstly, the original 5G RTT series are subjected to first-order differencing to obtain differential sequences with stronger stationarity. Then, a time series analysis-based variational mode decomposition–long short-term memory (VMD-LSTM) method is proposed to separately predict each differential sequence. Finally, the predicted results are subjected to inverse difference to obtain the predicted value of 5G RTT, and a predictive error of 4.481% indicates that the method performs better than LSTM and other methods. The prediction results could be used to evaluate network performance based on business requirements, reduce the impact of instruction packet loss, and improve the robustness of control algorithms. The proposed early warning accuracy metrics for control issues can also be used to indicate when to retrain the model and to indicate the setting of the control cycle. The field of industrial control, especially in the manufacturing industry, which requires low latency, will benefit from this analysis. It should be noted that the above analysis and prediction methods are also applicable to the R16 and R17 versions.