Later Stages Of Process Research Articles

Simple actions modulate context-dependent visual size perception at late processing stages

A simple button press towards a prime stimulus enhances subsequent visual search for objects that match the prime. The present study investigated whether this action effect is a general phenomenon across different task domains, and the underlying neural mechanisms. The action effect was measured in an unspeeded size-matching task, with the presentation of the central target and the surrounding inducers of the Ebbinghaus illusion together to one eye or separately to each eye, and when repetitive TMS was applied over right primary motor cortex (M1). The results showed that a prior key-press significantly reduced the illusion effect compared to passive viewing. Notably, the action effect persisted with dichoptic presentation of the Ebbinghaus configuration, but disappeared with the right M1 disruption. These results suggest that action guides visual perception to influence human behavior, which mainly affects the late visual processing stage and probably relies on feedback projections from the motor cortex.

Pre-ribosomal WDR74 module coordinates the early and late pre-rRNA processing stages for the NVL2-mediated regulation of 60S ribosome biogenesis

Pre-ribosomal WDR74 module coordinates the early and late pre-rRNA processing stages for the NVL2-mediated regulation of 60S ribosome biogenesis

Attentional differences in L2 learners’ word learning strategies between mobile-assisted and in-print reading: An eye tracking study

The present study combines three influential and interwoven areas of research (MALL, SLA, and psycholinguistics) into a cohesive research framework to explore whether and how medium and word exposure influence L2 learners’ incidental acquisition and online processing of new words using eye tracking methodology. Seventy-seven L2 learners engaged in reading a short novel containing new words of higher and lower exposure frequencies on a tablet or in print. We assessed L2 learners’ word learning accuracy using word form recognition, meaning recognition, and meaning recall tasks after reading. Eye tracking measures (including first-fixation duration, first-pass time, second-pass time, and total-reading time) were used to analyse visual attention allocated at the early and late stages of L2 learners’ lexical processing during reading. The results from ANCOVA analyses demonstrated the continued positive and significant effects of word exposure on L2 learners’ learning gains and attentive processing. Further, our findings indicated the negative effects of mobile-assisted reading on both accuracy and attention compared to in-print reading, with the latter readers achieving greater word learning gains in all three accuracy dimensions and employing an attentive reading strategy with more attentional resources allocated at the early stage and fewer ones at the late stage of lexical processing, as opposed to a skimming strategy observed in tablet users. From a pedagogical perspective, the cognitive costs associated with attentional deficits underscore issues on underlying cognitive mechanisms and highlight interventions requiring further exploration and consideration.

The Charactertistics and neural mechanisms of trait and state empathy in deaf individuals

Empathy deficiencies are prevalent among deaf individuals. It has yet to be determined whether they exhibit deficiencies in both trait empathy and state empathy, along with the effect of top-down attention. Here, the current study employed the IRI-C scale and physiological pain empathy tasks (A-P task and A-N task) to explore the temporal dynamics of neural activities when deaf individuals were processing second-hand painful/non-painful stimuli. For trait empathy, we found that deaf individuals have deficiencies in both emotional and cognitive empathy compared to their hearing counterparts. For state empathy, we found that deaf individuals showed stronger automatic emotional empathy responses and paid more cognitive evaluation resources. Moreover, the differential processing of empathy between deaf individuals and hearing individuals towards others’ pain could be regulated by top-down attention, which occurs both in the early and late processing stages of pain empathy.

Bacterial community succession and its correlation with the dynamics of flavor compound during processing of Enshi bacon

Enshi bacon, a famous specialty in Enshi Prefecture, Hu Bei, China, is renowned among customers for the nutritious content and distinct smoky flavor. This study explored the flavor and bacterial flora changes of Enshi bacon during processing and investigated the effect of bacteria on flavor formation. The results showed that the main genera in the pre-processing stage were Brochothrix, Lactococcus and Psychrobacter, which originated from raw meat. Salt-tolerant Staphylococcus and Cobetia were the dominant genera in the late stage of processing. Five free amino acids (aspartic acid, glutamic, alanine, valine, and lysine) were recognized as the flavor substances promoting taste, while aldehydes and phenols were the volatiles that contributed the most to odor. Particularly, compared to the smoking phase, phenolic content increased 2.25-fold at the end of fermentation through microbial action and tyrosine catabolism. The correlation study showed Staphylococcus and Lactococcus as fundamental functional genera that are essential for the formation of bacon flavor. This study provided reference data for the changes in flavor indicators and flora during the processing of Enshi Bacon, revealed the potential mechanism of core functional flora and characteristic flavor formation, and provided a theoretical basis for quality control of the manufacturing process.

Individual refresh ability modulates irrelevant speech effect in reading: Evidence from eye movements

This study randomly recruited 245 college students aged between 18 and 21 years old who had never studied Spanish and selected 60 subjects (30 with high refresh ability and 30 with low refresh ability) based on their scores on a refresh ability test. The mixed experimental design of 2 (refresh ability: high, low) ×3 (background sound: meaningful, meaningless, silent) was adopted. The Eyelink1000 eye tracker was used to record the eye movement data of the subjects when reading the sentences of scientific papers. At the same time, meaningful (news broadcasting) or meaningless (Spanish) background sounds are played. Based on this, the moderating effect of individual refresh ability with the impact of irrelevant speech was investigated. According to the results, the interaction between refresh ability and the meaningful background sound condition was significant, and it had an impact on the totalreading time, gaze count, regression count, total fixation time, and regression-path duration. However, the interaction with the meaningless background sound condition was not significant. The results showed that: (1) refresh ability significantly regulates the irrelevant speech effect in reading, compared with individuals with high refresh ability. The meaningful background sounds have a greater negative impact on the reading of individuals with low refresh ability; (2) the regulating effect of refresh ability occurs in the late stage of vocabulary processing and semantic integration. The results supported the attentional capture theory and found that the irrelevant speech effect in Chinese reading is modulated by individual refresh ability, which mainly acts on semantic components in late reading processing.

Grasping the Concept of an Object at a Glance: Category Information Accessed by Brief Dichoptic Presentation.

What type of conceptual information about an object do we get at a brief glance? In two experiments, we investigated the nature of conceptual tokening-the moment at which conceptual information about an object is accessed. Using a masked picture-word congruency task with dichoptic presentations at "brief" (50-60 ms) and "long" (190-200 ms) durations, participants judged the relation between a picture (e.g., a banana) and a word representing one of four property types about the object: superordinate (fruit), basic level (banana), a high-salient (yellow), or low-salient feature (peel). In Experiment 1, stimuli were presented in black-and-white; in Experiment 2, they were presented in red and blue, with participants wearing red-blue anaglyph glasses. This manipulation allowed for the independent projection of stimuli to the left- and right-hemisphere visual areas, aiming to probe the early effects of these projections in conceptual tokening. Results showed that superordinate and basic-level properties elicited faster and more accurate responses than high- and low-salient features at both presentation times. This advantage persisted even when the objects were divided into categories (e.g., animals, vegetables, vehicles, tools), and when objects contained high-salient visual features. However, contrasts between categories show that animals, fruits, and vegetables tend to be categorized at the superordinate level, while vehicles tend to be categorized at the basic level. Also, for a restricted class of objects, high-salient features representing diagnostic color information (yellow for the picture of a banana) facilitated congruency judgments to the same extent as that of superordinate and basic-level labels. We suggest that early access to object concepts yields superordinate and basic-level information, with features only yielding effects at a later stage of processing, unless they represent diagnostic color information. We discuss these results advancing a unified theory of conceptual representation, integrating key postulates of atomism and feature-based theories.

Unveiling the influence of persuasion strategies on cognitive engagement: an ERPs study on attentional search.

This study aimed to investigate how central versus peripheral persuasion methods, delivered through rational and emotional persuasion strategies, influence cognitive engagement and information processing during visual search tasks. Participants were allocated into four groups based on the media type (video vs. text) and the persuasion route (central vs. peripheral). The early and late stages of attentional processing were examined through the N1, P2, and P3 ERP components. The results demonstrated a pronounced N1 amplitude in response to text-based peripheral persuasion, indicating enhanced early attentional engagement. Additionally, parallel search tasks revealed a larger P3 amplitude for central versus peripheral routes, suggesting significant cognitive resource allocation during tasks requiring higher attention. These findings underscore the nuanced role of persuasive strategies in modulating attentional resources and cognitive processing. The study offers insights into designing more effective communication messages and highlights the potential for tailored persuasion approaches to influence audience engagement and information processing, with implications for public health campaigns and beyond.

Emotion perception through the nose: how olfactory emotional cues modulate the perception of neutral facial expressions in affective disorders

Humans can decode emotional states from the body odors of the conspecifics and this type of emotional communication is particularly relevant in conditions in which social interactions are impaired, as in depression and social anxiety. The present study aimed to explore how body odors collected in happiness and fearful conditions modulate the subjective ratings, the psychophysiological response and the neural processing of neutral faces in individuals with depressive symptoms, social anxiety symptoms, and healthy controls (N = 22 per group). To this aim, electrocardiogram (ECG) and HD-EEG were recorded continuously. Heart Rate Variability (HRV) was extracted from the ECG as a measure of vagal tone, event-related potentials (ERPs) and event-related spectral perturbations (ERPSs) were extracted from the EEG. The results revealed that the HRV increased during the fear and happiness body odors conditions compared to clean air, but no group differences emerged. For ERPs data, repeated measure ANOVA did not show any significant effects. However, the ERPSs analyses revealed a late increase in delta power and a reduced beta power both at an early and a late stage of stimulus processing in response to the neutral faces presented with the emotional body odors, regardless of the presence of depressive or social anxiety symptoms. The current research offers new insights, demonstrating that emotional chemosignals serve as potent environmental cues. This represents a substantial advancement in comprehending the impact of emotional chemosignals in both individuals with and without affective disorders.

Processing causal structure sentences in Mandarin Chinese: an eye movements study.

It remains uncertain whether causal structure prediction can improve comprehension in Chinese sentences and whether the position of the headword mediates the prediction effect. We conducted an experiment to explore the effect of causal prediction and headword position in Chinese sentence reading. Participants were asked to read sentences containing causal connectives with their eye movements recorded. In the experiment, we manipulated the causal structure of the sentence and the position of the headword. We found a promoting effect of causal structure on first-pass reading time and a hindering impact on total reading time. However, the effect was not mediated by the headword position. The results show that causal syntactic prediction facilitated early-stage processing and increased the integration cost in the late stage of Chinese sentence processing. These findings also support the constraint-based approach, which suggests an isolation between semantic and syntactic processing.

Neurophysiological effects of cognitive behavioral therapy in social anxiety: An ERP study using a dot-probe task

BackgroundSocial anxious individuals show attention bias towards emotional stimuli, this phenomenon is considered to be an important cause of anxiety generation and maintenance. Cognitive-behavioral therapy (CBT) is a standard psychotherapy for social anxiety disorder. CBT decreases attention biases by correcting the maladaptive beliefs of socially anxious individuals, but it is not clear whether CBT alters neurophysiological features of socially anxious individuals at early automatic and/or late cognitive strategy stage of attentional processing. MethodTo address this knowledge gap, we collected pre-treatment event-related potential data of 22 socially anxious individuals while they performed a dot-probe task. These participants then received eight weeks of CBT, and post-treatment ERP data were collected after completion of CBT treatment. We also included 29 healthy controls and compared them with individuals with social anxiety to determine the neural mechanisms underlying the effectiveness of CBT. ResultsParticipants’ social anxiety level was significantly alleviated with CBT. ERP results revealed that (1) compared to pre-treatment phase, P1 amplitudes induced by probes significantly decreased at post-treatment phase, whereas P3 amplitudes increased at post-treatment phase; the P1 amplitudes induced by probes following happy-neutral face pairs in socially anxious individuals after treatment was significantly different with that in healthy controls; (2) amplitude of components elicited by face pairs did not change significantly between pre-treatment and post-treatment phases; (3) changes of Liebowitz Social Anxiety Scale were positively correlated with changes of P1 amplitude, and negatively correlated with changes of N1 amplitude. LimitationsOur sample was university students and lacked randomization, which limits the generalizability of the results. ConclusionThe present results demonstrated that CBT may adjust cognitive strategies in the later stage of attentional processing, indicating by changed ERPs appeared in probe-presenting stage for social anxiety.

Orthogonal characterization of rAAV9 reveals unexpected transgene heterogeneity

Recombinant adeno-associated virus (rAAV) is the most widely used viral vector for in vivo human gene therapy. To ensure safety and efficacy of gene therapy products, a comprehensive analytical profile of the rAAVs is needed, which provides crucial information for therapeutic development and manufacturing. Besides information on rAAV quantities and possible contaminating DNA and protein species, assessing rAAV quality is of utmost importance. In vitro biopotency and methods to determine the full/empty ratio of rAAV capsids are commonly applied, but methods to assess the integrity of the viral genome are still rarely used. Here we describe an orthogonal approach to characterize rAAV quality. Two biologically different rAAV9s from different stages of the bioprocess, generated each with two different transfection reagents, were investigated. In vitro biopotency tests in all cases demonstrated that rAAV9s generated with transfection reagent FectoVIR® possessed a higher biological activity. Mass-based analytical methods, such as sedimentation velocity analytical ultracentrifugation (AUC) and mass photometry, showed a high share of full capsids (>80 %) at late process stages but did not detect any differences in the rAAV9s from the different transfection reagents. Multiplex dPCR and Nanopore long-read sequencing both demonstrated that, also in late-stage process samples, sample heterogeneity was relatively high with a rather small share of full-length transgenes of ∼10–40 %. Intriguingly, both methods detected a higher share of complete transgenes in rAAV9 generated with transfection reagent FectoVIR® instead of Polyethylenimine (PEI), and thereby explain the differences already observed in the biopotency assays. This study therefore emphasizes the necessity to utilize multiple, orthogonal methods to gain a better understanding of recombinantly manufactured AAVs.

The effects of different types of emotion words on emotion picture processing - evidence from event-related potential.

Emotional information can be seen everywhere in daily life. Research on emotional words often employs lexical decision tasks to judge the veracity of words, involving only superficial processing and not the deep processing of emotional significance. Therefore, the purpose of this study is to explore the effect of types of emotional words on the processing of emotional pictures. Participants were publicly recruited for a button-press experiment to discuss the impact of emotional words on the processing of emotional pictures from both behavioral and physiological mechanisms. The results of experiment 1 show: (a) in terms of reaction time, the processing speed for negative emotional words was slower, with longer reaction times; (b) In terms of accuracy, positive emotional words had a higher correct rate than negative emotional words. The results of experiment 2 found: (a) a significant main effect of emotional word type in the late processing stage; (b) a significant interaction between emotional word type and congruency. Previously presented emotional words affect the processing of subsequently presented emotional pictures, with differences in the processing of the four types of words, indicating a significant role of language in emotional processing.

Human brain dynamics dissociate early perceptual and late motor-related stages of affordance processing.

Affordances, the opportunities for action offered by the environment to an agent, are vital for meaningful behaviour and exist in every interaction with the environment. There is an ongoing debate in the field about whether the perception of affordances is an automated process. Some studies suggest that affordance perception is an automated process that is independent from the visual context and bodily interaction with the environment, whereas others argue that it is modulated by the visual and motor context in which affordances are perceived. The present paper aims to resolve this debate by examining affordance automaticity from the perspective of sensorimotor time windows. To investigate the impact of different forms of bodily interactions with an environment, that is, the movement context (physical vs. joystick movement), we replicated aprevious study on affordance perception in which participants actively moved through differently wide doors in an immersive 3D virtual environment. In the present study, wedisplayed the same environmenton a 2D screen with participants moving through doors of different widths using the keys on a standard keyboard. We compared components of the event-related potential (ERP) from the continuously recorded electroencephalogram (EEG) that were previously reported to be related to affordance perception of architectural transitions (passable and impassable doors). Comparing early sensory and later motor-related ERPs, our study replicated ERPs reflecting early affordance perception but found differences in later motor-related components. These results indicate a shift from automated perception of affordances during early sensorimotor time windows to movement context dependence of affordance perception at later stages, suggesting that affordance perception is a dynamic and flexible process that changes over sensorimotor stages.

An ERP study on the late stage of Chinese metaphor processing.

Psycholinguistic models of metaphor processing remain a subject of debate. A prime-probe design using Chinese materials with a specific time span (300 ms) was applied to test the mechanisms of metaphor processing. Conventional and familiarized metaphors were designed as primes, followed by a probe word semantically related to the prime metaphor (MT), a probe word related to the literal meaning of the final word of the prime metaphor (LT), control/unrelated probe word (UT), or non-word. Event-related potentials (ERPs) elicited by the probes were recorded to examine metaphor processing. In N400, results revealed that UT and LT elicited significantly more negative waveforms than MT in both primes. MTs and LTs showed no difference between conventional and familiarized metaphors, suggesting that metaphorical meaning may be accessed directly, regardless of whether conventional or familiarized metaphors. The results were generally compatible with the direct processing model.

Visual periodicity reveals distinct attentional signatures for face and non-face categories.

Observers can selectively deploy attention to regions of space, moments in time, specific visual features, individual objects, and even specific high-level categories-for example, when keeping an eye out for dogs while jogging. Here, we exploited visual periodicity to examine how category-based attention differentially modulates selective neural processing of face and non-face categories. We combined electroencephalography with a novel frequency-tagging paradigm capable of capturing selective neural responses for multiple visual categories contained within the same rapid image stream (faces/birds in Exp 1; houses/birds in Exp 2). We found that the pattern of attentional enhancement and suppression for face-selective processing is unique compared to other object categories: Where attending to non-face objects strongly enhances their selective neural signals during a later stage of processing (300-500ms), attentional enhancement of face-selective processing is both earlier and comparatively more modest. Moreover, only the selective neural response for faces appears to be actively suppressed by attending towards an alternate visual category. These results underscore the special status that faces hold within the human visual system, and highlight the utility of visual periodicity as a powerful tool for indexing selective neural processing of multiple visual categories contained within the same image sequence.

Neural correlates of emotion-label vs. emotion-laden word processing in late bilinguals: evidence from an ERP study

ABSTRACT The brain processes underlying the distinction between emotion-label words (e.g. happy, sad) and emotion-laden words (e.g. successful, failed) remain inconclusive in bilingualism research. The present study aims to directly compare the processing of these two types of emotion words in both the first language (L1) and second language (L2) by recording event-related potentials (ERP) from late Chinese-English bilinguals during a lexical decision task. The results revealed that in the early word processing stages, the N170 emotion effect emerged only for L1 negative emotion-laden words and L2 negative emotion-label words. In addition, larger early posterior negativity (EPN) was elicited by emotion-laden words than emotion-label words in both L1 and L2. In the later processing stages, the N400 emotion effect was evident for L1 emotion words, excluding positive emotion-laden words, while it was absent in L2. Notably, L1 emotion words elicited enhanced N400 and attenuated late positive complex (LPC) compared to those in L2. Taken together, these findings confirmed the engagement of emotion, and highlighted the modulation of emotion word type and valence on word processing in both early and late processing stages. Different neural mechanisms between L1 and L2 in processing written emotion words were elucidated.

Morality or competence is more important? The effect of evaluation dimensions on ERP responses to neutral faces depends on contextual valence and self-relevance

Recent studies have shown that the processing of neutral facial expressions could be modulated by the valence and self-relevance of preceding verbal evaluations. However, these studies have not distinguished the dimension (i.e., morality and competence) from verbal evaluations. In fact, there is a hot controversy about whether morality or competence receives more weight. Therefore, using the ERP technique, the current study aimed to address this issue by comparing the influence of morality and competence evaluations on behavioral and neural responses to neutral facial expressions when these evaluations varied with contextual valence and self-relevance. Our ERP results revealed that the early EPN amplitudes were larger for neutral faces after receiving evaluations about self relative to evaluations about senders. Moreover, the EPN was more negative after a competence evaluation relative to a morality evaluation when these evaluations were positive, while this effect was absent when these evaluations were negative. The late LPP was larger after a morality evaluation compared to a competence evaluation when these evaluations were negative and directed to self. However, no significant LPP effect between morality and competence evaluations was observed when these evaluations were positive. The present study extended previous studies by showing that early and late processing stages of faces are affected by the evaluation dimension in a top-down manner and further modulated by contextual valence and self-relevance.

Visual similarity effects in the identification of Arabic letters: evidence with masked priming

Abstract Research using masked priming and parafoveal preview techniques has shown that visual letter similarity has an impact on word processing during the initial stages in Latin-derived scripts. However, these effects appear to be absent in Arabic. One reason for this discrepancy could be attributed to the distinctive features of the Arabic script, which includes numerous letters sharing a basic form while varying in the location or number of diacritics. To shed light on this issue, the present study employed Arabic letters rather than words in two masked priming experiments: an alphabetic decision task and a letter-matching task. Both experiments showed that visually similar letters were more effective as primes than visually dissimilar letters. These findings suggest that the processes of letter identification in Arabic and Latin scripts may be roughly alike, implying that differences in visual letter similarity across scripts may arise at later stages of processing.

Brain mechanisms underlying the emotion processing bias in treatment-resistant depression

Depression is associated with a cognitive bias towards negative information and away from positive information. This biased emotion processing may underlie core depression symptoms, including persistent feelings of sadness and a reduced capacity to experience pleasure. The neural mechanisms responsible for this biased emotion processing remain unknown. Here we had a unique opportunity to record stereotactic electroencephalography signals in the amygdala and prefrontal cortex (PFC) from 5 patients with treatment-resistant depression (TRD) and 12 patients with epilepsy (as control) while they participated in an affective bias task in which happy and sad faces were evaluated. First, compared with the control group, patients with TRD showed increased amygdala responses to sad faces in the early stage (around 300 ms) and decreased amygdala responses to happy faces in the late stage (around 600 ms) following the onset of faces. Furthermore, during the late stage of happy-face processing, alpha-band activity in the PFC as well as alpha-phase locking between the amygdala and the PFC were significantly greater in patients with TRD compared with the control group. The increased amygdala activation during the early stage of sad-face processing suggests an overactive bottom-up processing system in TRD. Meanwhile, the reduced amygdala response during the late stage of happy-face processing could be attributed to increased top-down inhibition by the PFC through alpha-band oscillation, which may be relieved following deep brain stimulation in the subcallosal cingulate and the ventral capsule/ventral striatum.