Land surface temperature (LST) is one of the key parameters in the process of energy exchange between the land surface and atmosphere, and thermal infrared (TIR) remote sensing is an important approach to efficiently obtain LST over a large area. Algorithms for retrieval of LST from TIR remote sensing data have been studied for decades, and the split-window (SW) algorithm can directly eliminate atmospheric effects by using the brightness temperature at the top of the atmosphere in two adjacent TIR channels and thus is widely applied. Landsat-9, the latest launch in the Landsat series of satellites, provides 2-channel TIR images with the same 100m spatial resolution as Landsat-8, and it is meaningful to develop the SW algorithm for LST retrieval using Landsat-9 data. In this paper, four SW algorithms were developed, and the accuracy and noise sensitivity of the results under different observation conditions were compared based on the simulation dataset to select the algorithm with the best performance. The ground measurement data under different land cover types and the global Landsat-9 LST products, produced by the single-channel algorithm, were selected to verify the accuracy of the proposed algorithm. The results show that the ground validation accuracy is about 1.574 K, better than the Landsat-9 existing LST product. Moreover, the retrieved LST images have similar spatial distribution to the Landsat-9 LST products, with RMSEs from 0.31 K to 2.87 K in various regions.