Late Knee Osteoarthritis Research Articles

Screening of key pathogenic genes in advanced knee osteoarthritis based on bioinformatics analysis.

BackgroundAt present, the progression mechanism of knee osteoarthritis (KOA) has not been fully elucidated, and there is a clinical need for late KOA-specific diagnostic markers to provide reference for preventive treatment. This study aimed to analyze the sequencing results of early- and late-stage KOA synovial tissue based on the key genes of late-stage KOA in combination with a machine learning algorithm.MethodsThe whole transcriptome sequencing results of synovial tissue from KOA patients (GSE176223 and GSE32317) were downloaded from the gene expression omnibus (GEO) database. Thirty-nine early KOA synovial tissue samples and 31 late KOA synovial tissue samples were included in this study. The diagnostic criteria and baseline data balance of early and late KOA were referred to the data source literature, and the two groups of data had good baseline data balance. R software (V3.5.1) and R packages were used for screening and enrichment analysis of differentially expressed genes (DEGs). The key genes were screened by weighted correlation network analysis (WGCNA) and least absolute shrinkage and selection operator (LASSO) regression analysis. A receiver operating characteristic curve (ROC) curve was used to evaluate the diagnostic efficacy of key genes for advanced KOA.Results A total of 211 DEGs related to knee arthritis were screened out. Compared with synovial tissue of early knee arthritis, 111 genes were upregulated and 100 genes were downregulated in the synovial tissue of late knee arthritis. Sixty-six key genes were screened out through WGCNA and 34 key genes were screened out in the LASSO analysis. The genes obtained by the two algorithms combined with three overlapping genes, namely interleukin- 6 (IL-6), C-X-C chemokine ligand 12 (CXCL12), and macrophage migration inhibitor factor (MIF). The areas under the ROC curves of CXCL12, IL-6, and MIF were 0.96, 0.944, and 0.961, respectively (P<0.001).ConclusionsIL-6, CXCL12, and MIF are the key pathogenic genes of KOA, which have good diagnostic efficacy for advanced KOA.

Does Bisphosphonate Increase the Sclerosis of Tibial Subchondral Bone in the Progression of Knee Osteoarthritis-A Propensity Score Matching Cohort Study Based on Osteoarthritis Initiative.

Bisphosphonate has great potential in KOA therapy, but whether the anti-resorption mechanism of bisphosphonate aggravates sclerosis of subchondral bone remains unclear. We found that bisphosphonate use did not increase sclerosis of subchondral bone in established KOA, perhaps resolving some concerns about bisphosphonate in patients with KOA.Introduction: Most studies have focused on the protective effect of bisphosphonate on early knee osteoarthritis (KOA) through its anti-resorption mechanism in osteoclasts. However, late KOA has a decreased rate of resorption, which is the opposite of early KOA. The risk of subchondral bone sclerosis in late KOA after using bisphosphonate has not been investigated using morphometry.Methods: Forty-five patients who had ever used bisphosphonate (or 33 patients with current use) were matched with controls through propensity matching methods, including age, body mass index (BMI), sex, health status (12-Item Short Form Survey physical health score), physical activity level (Physical Activity Scale for the Elderly score), vitamin D use, and calcium use. At the baseline and 12-month (or 18-month) follow-up, bone mineral density (BMD) of the tibia and hip was measured by dual-energy X-ray absorptiometry (DXA), and medial tibial subchondral bone morphometry: bone volume fraction (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp) were calculated based on 3-T trabecular MRI. Data were obtained from the Bone Ancillary Study in the Osteoarthritis Initiative (OAI) project.Results: The yearly percentage change in hip BMD of the current bisphosphonate-use group was significantly greater than that of the non-bisphosphonate-use group (0.7% vs. −1%, P = 0.02). The other outcomes (BV/TV, Tb.N, Tb.Sp, Tb.Th, tibia medial BMD, and tibia lateral BMD) between the two groups presented no significant difference. The non-bisphosphonate-use group experienced a significant increase in Tb.Th [2%, 95% CI = (1%, 4%), P = 0.01], while the bisphosphonate-use group presented no significant change [1%, 95% CI = (−2%, 4%), P = 0.54].Conclusions: Bisphosphonate use did not increase sclerosis of subchondral bone in established KOA. Bisphosphonate might have a stage-dependent effect on subchondral bone in KOA initiation and progression.

Health-related quality of life and its influencing factors in Chinese with knee osteoarthritis.

To evaluate the health-related quality of life (HRQoL) of knee osteoarthritis (KOA) patients in China, compare their HRQoL with norm population, and examine the associations between the potential influencing factors and HRQoL. A cross-sectional study was conducted in 912 KOA patients from 4 provinces between March and November 2017. All participants were diagnosed according to the National Institute for Health and Care Excellence (NICE)'s criterion or the Chinese Medical Association (CMA)'s criterion. Recruited patients were surveyed for HRQoL using the 12-item Short Form (SF-12) and sociodemographic, disease-related factors. We assessed the associations between potential influencing factors and HRQoL using multiple linear regression models. Among the KOA patients, the mean physical component summary (PCS) of HRQoL was 40.91 ± 11.62, lower than norm (P < 0.001). And the mean mental component summary (MCS) was 50.25 ± 11.99, similar to the norm. Patients who were older (β = - 0.155, P = 0.001), women (β = - 4.589, P < 0.001), had ever been treated (β = - 2.426, P = 0.006), had longer course of KOA (β = - 0.164, P = 0.012), and were in early stage (β = - 2.968, P = 0.001) or progressive/late stage (β = - 7.636, P < 0.001) showed lower scores of PCS. Patients who lived in Hunan (β = 4.988, P < 0.001) and had education levels of junior high school (β = 3.134, P = 0.012) or senior high school and above (β = 3.050, P = 0.010) had better mental status. Those suffered from non-knee pains (β = - 2.308, P = 0.027) and with progressive or late KOA (β = - 4.690, P = 0.016) had lower MCS scores. KOA patients had worse physical condition. The mental and physical health of patients were affected by different factors. Targeted management measures should be taken to improve their HRQoL.

Identification of synovial fluid MIRNA signature in knee osteoarthritis: differentiating early and late knee osteoarthritis

Identification of synovial fluid MIRNA signature in knee osteoarthritis: differentiating early and late knee osteoarthritis

Ultrasonographic findings in young adult patients with early osteoarthritis (OA) of the knee. Preliminary data.

Purpose: Introduction. The OA is a chronic and multi-factorial disease; it is characterized by gradual loss of articular cartilage. The accepted method for assessing and doing OA diagnosis is a conventional radiography. When the clinicians assessed a knee radiography and give a diagnosis of OA, maybe it is late, because the patients have some months or years that started the degenerative process in cartilage. In recent years the musculoskeletal ultrasound (MSUS) has been used to obtain data about late knee OA, however there are few article published, which described morpho-structural changes in the MSUS in patients with early knee OA. Objectives: To estimate the prevalence of morpho-structural changes (presence of synovitis, effusion, osteophytes and meniscal extrusion) using MSUS in early knee OA patients. Evaluate the relationship between these ultrasound findings and quality of life and function in knee OA patients. Methods: Patients and Methods: We used preliminary data from the study of the impact of metabolic syndrome (MS) in patients with OA, which is carried out at the National Institute of Rehabilitation. Briefly, inclusion criteria: adult patients under 55 years of age and criteria for knee OA from the ACR and the radiography score 1 to 2 of Kellgren and Lawrence classification. Patients with autoimmune inflammatory diseases, fibromyalgia, illness crystals and previous surgery were excluded. All patients underwent to knee MSUS to evaluate the presence of synovitis, effusion, osteophytes and meniscus extrusion. Epidemiological data were collected from patients, the EuroQoL were used to evaluate the quality of life and WOMAC scale was applied to evaluate the knee function. The results were analyzed using descriptive and bivariate test, parametric and no-parametric statistical tests were used. Results: We studied 72 patients, mean age 45 years ± 7.4, 83% female gender, education (years) mean 10.5 ± 3.8, Body Mass Index 30.6 ± 4.9. Score for WOMAC were: pain 1.4, stiffness 1.5, physical function 1.3. EuroQol 0.55 in male population vs. 0.69 in women population. The table 1 show MSUS findings. Conclusions: morpho-structural findings by MSUS were found in a high prevalence of patients with knee OA classified by radiology in early stages. This shows that although radiology is the most used method for classification of disease, it does not evaluate an early OA, further studies are required to detect early changes of OA.Table IPrevalence of musculoskeletal ultrasound findings in OA patientsUltrasound knee siteSynovitis % (mm)MeniscusExtrusion % (mm)Site of OsteophytesMedial / Lateral(%)SP* Right18 (7.9)Medial Right8 (2.8)Femoral Right35 / 63SP* Left28 (8.2)Medial Left6 (3.8)Femoral Left63 / 63Medial Right20 (4.3)Lateral Right19 (3.6)Tibial Right46 / 63Medial Left20 (4.1)Lateral Left19 (3.4)Tibial Left57 / 63Lateral Right48 (4.6)Lateral Left43 (4.2) Open table in a new tab

John Anthony Clark Wilson

<h3>Background</h3> The role and the significance of calcium crystals (CC) in synovial inflammation and in Osteoarthritis (OA) progression is somewhat debated. Calcium pyrophosphate (CPP) and basic calcium phosphate (BCP) are...