Laser Tweezers Research Articles

Trapping light, revealing properties: Laser trapping as a powerful tool for photoluminescence spectroscopy

Laser trapping, a non-contact technique for manipulating microscopic objects, has gained prominence in scientific research. When coupled with fluorescence spectroscopy, it offers a powerful tool for exploring material properties. This study demonstrates the application of laser trapping in the crystallization of MAPbBr3 perovskites and its simultaneous use for photoluminescence imaging. MAPbBr3 perovskites are a class of materials with exceptional optical properties, making them attractive for various optoelectronic applications. However, conventional excitation methods using UV light can lead to phase segregation and mechanical distortion of these materials. Two-photon excitation, on the other hand, offers advantages such as deeper penetration and reduced scattering interference. In this research, we utilize a 1064 nm continuous wave laser for both trapping and excitation purposes. The MAPbBr3 perovskite, with its absorption band ranging from 400 to 540 nm, exhibits two-photon excitation at 532 nm. By focusing the laser beam at the air-solution interface, we successfully crystallize MAPbBr3 perovskites from an unsaturated precursor solution. Simultaneously, the same laser source is used for photoluminescence imaging, allowing for real-time analysis of the crystal's emission properties. This approach eliminates the need for additional excitation sources and simplifies the experimental setup. The combination of laser trapping and two-photon excitation opens up new possibilities for studying perovskite materials. It provides a gentle and non-invasive method for manipulating and characterizing hybrid perovskites materials, paving the way for advancements in various fields such as optoelectronics and energy harvesting.

Switchable Optical Trapping of Mie‐Resonant Phase‐Change Nanoparticles

AbstractOptical tweezers revolutionized the manipulation of nanoscale objects. Typically, tunable manipulations of optical tweezers rely on adjusting either the trapping laser beams or the optical environment surrounding the nanoparticles. Here, tunable and switchable trapping using nanoparticles made of a phase‐change material (vanadium dioxide or VO2) are achieved. By varying the intensity of the trapping beam, transitions of the VO2 between monoclinic and rutile phases are induced. Depending on the nanoparticles' sizes, they exhibit one of three behaviors: small nanoparticles (in the settings, radius wavelength ) remain always attracted by the laser beam in both material phases, large nanoparticles () remain always repelled. However, within the size range of , the phase transition of the VO2 switches optical forces between attractive and repulsive, thereby pulling/pushing them toward/away from the beam center. The effect is reversible, allowing the same particle to be attracted and repelled repeatedly. The phenomenon is governed by optical Mie modes of the nanoparticles and their alterations during the phase transition of the VO2. This work provides an alternative solution for dynamic optical tweezers and paves a way to new possibilities, including optical sorting, light‐driven optomechanics and single‐molecule biophysics.

Chiral, Topological, and Knotted Colloids in Liquid Crystals

The geometric shape, symmetry, and topology of colloidal particles often allow for controlling colloidal phase behavior and physical properties of these soft matter systems. In liquid crystalline dispersions, colloidal particles with low symmetry and nontrivial topology of surface confinement are of particular interest, including surfaces shaped as handlebodies, spirals, knots, multi-component links, and so on. These types of colloidal surfaces induce topologically nontrivial three-dimensional director field configurations and topological defects. Director switching by electric fields, laser tweezing of defects, and local photo-thermal melting of the liquid crystal host medium promote transformations among many stable and metastable particle-induced director configurations that can be revealed by means of direct label-free three-dimensional nonlinear optical imaging. The interplay between topologies of colloidal surfaces, director fields, and defects is found to show a number of unexpected features, such as knotting and linking of line defects, often uniquely arising from the nonpolar nature of the nematic director field. This review article highlights fascinating examples of new physical behavior arising from the interplay of nematic molecular order and both chiral symmetry and topology of colloidal inclusions within the nematic host. Furthermore, the article concludes with a brief discussion of how these findings may lay the groundwork for new types of topology-dictated self-assembly in soft condensed matter leading to novel mesostructured composite materials, as well as for experimental insights into the pure-math aspects of low-dimensional topology.

Noninvasive Raman spectroscopy for the detection of rice bacterial leaf blight and bacterial leaf streak

Plant diseases pose significant threats to agricultural yields and are responsible for nearly 20 % of losses in total food production. Therefore, the rapid detection of plant pathogens is critically important for preventing the rapid development of plant diseases and minimizing crop damage. Raman spectroscopy (RS) has been shown to be effective for detecting living biological samples. Compared with traditional detection methods, RS is fast, sensitive, and non-destructive; it also does not require sample labeling. In this study, we used Laser tweezers Raman spectroscopy combined with convolutional neural networks to detect two closely related strains of bacteria, Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc), exuded from bacteria-infected rice leaves. The accuracy of this technique was 97.5 %. For the application of RS in the field, we used the portable Raman spectrometer to detect mock-inoculated as well as Xoo- and Xoc-infected rice leaves at different disease courses. The identification accuracy via this technique was 87.02 % in the early stage, in which no obvious symptoms were apparent. This method also revealed spectral differences in rice leaves caused by the two bacteria, which could be leveraged for subsequent analysis of the molecular mechanism of infection. Our results indicate that RS is a promising approach for the early detection of bacterial diseases in rice in the field, as well as for in-depth single-cell analysis in laboratory settings.

All-Optical Trapping and Programmable Transport of Gold Nanorods with Simultaneous Orientation and Spinning Control.

Gold nanorods (GNRs) are of special interest in nanotechnology and biomedical applications due to their biocompatibility, anisotropic shape, enhanced surface area, and tunable optical properties. The use of GNRs, for example, as sensors and mechanical actuators, relies on the ability to remotely control their orientation as well as their translational and rotational motion, whether individually or in groups. Achieving such particle control by using optical tools is challenging and exceeds the capabilities of conventional laser tweezers. We present a tool that addresses this complex manipulation problem by using a curve-shaped laser trap, enabling the optical capture and programmable transport of single and multiple GNRs along any trajectory. This type of laser trap combines confinement and propulsion optical forces with optical torque to transport the GNRs while simultaneously controlling their rotation (spinning) and orientation. The proposed system facilitates the light-driven control of GNRs and the quantitative characterization of their motion dynamics including transport speed, spinning frequency, orientation, and confinement strength. We experimentally demonstrate that remote control of the GNRs can be achieved both near a substrate surface (2D trapping) and deep within the sample (3D all-optical trapping). The motion dynamics of two sets of off-resonant GNRs, possessing similar aspect ratios but different resonance wavelengths, are analyzed to highlight the role played by their optical and mechanical properties in the optical manipulation process. The experimental results are supported by a theoretical model describing the observed motion dynamics of the GNRs. This optical manipulation tool can significantly facilitate applications of light-driven nanorods.

Charge radii of thallium isotopes near the N=126 shell closure

The changes in the mean-squared charge radius of Tlg209 (N=128) and Tlm207 (N=126) relative to Tl205 have been measured for the first time using the in-source laser resonance-ionization spectroscopy technique with the Laser Ion Source and Trap (LIST) at ISOLDE (CERN). The application of the LIST suppresses the dominant background from isobaric francium isotopes and allows access to thallium nuclides with A⩾207. The characteristic kink in the charge radii at the N=126 neutron shell closure, as well as the odd-even effect similar to that in the adjacent bismuth, lead, and mercury isotopic chains, have been observed. The self-consistent theory of finite Fermi systems based on the energy density functional by Fayans reproduces the behavior of charge radii in these isotopic chains near N=126. The comparison with calculations in the framework of the relativistic mean field (RMF) approach is also presented. In the case of the Fayans functional it is a specific form of pairing interaction with the dependence on the density gradient that is essential to provide agreement with the experimental charge radii. In particular, the kink is reproduced without the inversion of g9/2 and i11/2 neutron single-particle states, which is a prerequisite to correctly describe the kink in the RMF models. Published by the American Physical Society 2024

Accessible Double Nanohole Raman Tweezer Analysis of Single Nanoparticles.

Raman spectroscopy allows for material characterization of nanoparticles; however, probing individual nanoparticles requires an efficient way of isolating and enhancing the signal. Past works have used optical trapping with nanoapertures in metal films to measure the Raman spectra of individual nanoparticles; however, those works required custom laser tweezer systems that provided a transmission signal to verify trapping events as well as costly top-down nanofabrication. Here, we trapped Titania nanoparticles in a commercial Raman system using double nanoholes (DNH) and measured their spectra while trapped. The microscope camera allowed for measuring the trapping event in reflection mode, and a simultaneous Raman spectrum was recorded to allow for material characterization. The Raman signal was comparable to a past work that used particles a million times larger in volume without utilizing double nanoholes, and all other features were similar. The DNHs were created with a colloidal lithography technique and identified in the microscope, as confirmed by electron microscopy registration. Therefore, this approach allows a simple way of characterizing the Raman signal of individual nanoparticles while in solution by using existing commercial Raman systems.

Amphibious Hybrid Laser Tweezers for Fluid and Solid Domains.

Optical trapping is a potent tool for achieving precise and noninvasive manipulation of small objects in a vacuum and liquids. However, due to the substantial disparity between optical forces and interfacial adhesion, target objects should be suspended in fluid environments, rendering solid contact surfaces a restricted area for conventional optical tweezers. In this work, by relying on a single continuous wave (CW) laser, we demonstrate an optical manipulation system applicable for both fluid and solid domains, namely, amphibious hybrid laser tweezers. The key to our system lies in modulating the intensity of the CW laser with duration shorter than the dynamic thermal equilibrium time within objects, wherein strong thermal gradient forces with ∼6 orders of magnitude higher than the forces in optical tweezers are produced, enabling moving and trapping micro/nano-objects on solid interfaces. Thereby, CW laser-based optical tweezers and pulsed laser-based photothermal shock tweezers are seamlessly fused with the advantages of cost-effectiveness and simplicity. Our concept breaks the stereotype that CW lasers are limited to generating tiny forces and instead achieve ultrawide force generation spanning from femto-newtons (10-15 N) to (10-6 N). Our work expands the horizon of optical manipulation by seamlessly bridging its applications in fluid and solid environments and holds promise for inspiring optical manipulation techniques to perform more challenging tasks, which may unearth application scenarios in diverse fields such as fundamental physical research, nanofabrication, micro/nanorobotics, biomedicine, and cytology.

Fast lipid vesicles and dielectric particles migration using thermal-gradient-induced forces

Abstract Lipid vesicles are small biological particles that can be used for both targeted drug delivery systems and clinical studies. Their optical manipulation, however, is limited by the small difference in refractive indices with the surrounding medium, as well as the requirement for high laser trapping powers. In this work, we combine gradient force and thermal forces to deliver and trap individual lipid vesicles with low-trapping laser powers. The total optothermal force exerted on liposomes causes them to migrate rapidly toward the laser focus with a high average migration velocity of 1.77 µm s−1 under 7.3% w/v polyethylene glycol (PEG) concentration and low trapping laser power of 1 mW. A high normalized experimental trap stiffness of 0.88 (pN µm) mW−1 was obtained at 7.3% w/v PEG/water solution. This work may open new ways for bioparticle sorting and manipulation with potential applications in cellular studies, drug delivery, biosensing, and medicine.

Laser Tweezers Raman Spectroscopy and its application in bio-chemical sensing

Laser Tweezers Raman Spectroscopy (LTRS) technology combines Raman spectroscopy and optical tweezers technology, providing a new way to research in single cell analysis and microbial classification. Driven by the development of precision spectroscopy and deep learning techniques, LTRS plays an increasingly important role in biomedical research, clinical diagnosis and environmental research. For example, it can be used to detect cancer cells, analyse bacterial infections, and monitor harmful environmental substances. In this paper, we review the basic principles of LTRS technology through literature reading and case studies, explain how the technology combines the advantages of optical tweezers and Raman spectroscopy in order to provide a non-destructive, label-free and susceptible single-cell analysis method, summarize the latest development of LTRS technology in recent years, as well as its applications in the field of biochemical sensing, and finally discuss the problems it is currently facing and look forward to its future development direction. Finally, the current problems faced by LTRS are discussed, and the direction of its future development is anticipated.

Probing elastic anisotropy on entropic interfaces

Liquid-liquid phase separation is vital in the formation of many biomolecular condensates, including cellular membraneless organelles. When semiflexible macromolecules are involved, the process switches from mostly enthalpy- to dominantly entropy-driven, changing the intrinsic nature of the interfaces and bringing new physical traits. Here, we present a concept to address the complexity of entropic interfaces where laser trapping and microscopy are combined to capture the behavior of probe colloidal particles onto liquid crystalline droplet surfaces. Hertz-Frank-Oseen contact theory is extended to calculate the elasticity of the droplet based on particle trajectories. We show that this method is sensitive enough to resolve the anisotropic elasticity originating from the collective molecular orientation of constitutive semiflexible mesogens, introducing a possible tool to assess biomechanics of cellular and molecular condensates. Published by the American Physical Society 2024

Ultra-narrow-linewidth hybrid-integrated self-injection locked laser at 780 nm

Narrow-linewidth lasers are essential across a wide range of applications, including classical and quantum sensing, trapped ion systems, position/navigation/timing systems, optical clocks, and microwave frequency synthesizers. In the visible and near-visible spectrum, low-noise lasers are particularly important for laser trapping and cooling techniques, which are vital for trapped ion quantum computing, sensing, and atomic clocks. In this context, our work showcases a hybrid-integrated narrow-linewidth laser that operates at 780 nm, achieving a self-heterodyne linewidth of 105 Hz. To validate the experimental results, we performed a numerical analysis that combines insights from a many-body theory applied to the gain region with a travelling-wave model to capture the laser dynamics. Our investigation further delves into how the linewidth of the self-injection locked lasers is influenced by the parameters of micro-ring resonators, aiming to assess the potential for achieving Hz-level integrated laser linewidths at 780 nm. This work not only demonstrates the technical feasibility of Hz-level narrow-linewidth lasers but also lays the groundwork for future explorations in the field.

Nanoscopic wetting behaviour of single oil droplets on a fibre

Multiphase fluids that contain dispersed oil droplets are fundamental to many industrial processes and formulated products. In the present work, the surface wetting and interaction between an emulsion droplet and a single fibre were quantitatively investigated as a function of surfactant chemistry, pH, and salt concentration using an optical tweezers apparatus. Silicone droplets were emulsified in water and stabilised by surfactants. An individual droplet was captured using an optical tweezers setup to allow a controlled contact with a fibre surface. During the approach process, the surface interaction was measured and spreading behaviour was quantified after contact was made between the droplet and fibre. Salt concentration was increased to change the surface interaction behaviour. The pH and ζ-potential of silicone emulsions decreased from 9.5 to 6.0 and from 39 mV to − 15 mV, respectively, when increasing salt concentration for both of the surfactants studied, SDS and CTAB. The attraction between the fibre and a silicone oil droplet was found to increase as the electrostatic repulsion was suppressed with an increased salt concentration. This was confirmed by an increased number of droplet-fibre adhesion events and in some cases droplet spreading on the fibre. Such observations for adhesion are based on electrostatic attractions exceeding the maximum force that can be exerted by the trapping laser. When adhesion was not observed, attraction could still be recorded through a thin-film hydrodynamic suction effect during the process of retraction, which becomes more pronounced on the polyester fibre. Our results provide a novel method to directly quantify the surface interaction and wetting of liquid droplets on microscopic fibres simultaneously, which could be valuable when investigating formulated products that involve emulsion droplets.

Effect of Organic Solvent on the Mass Transfer Mechanism of Coumarin 102 in a Single Octadecylsilyl Silica Gel/Organic Solvent-Water System by Laser Trapping and Fluorescence Microspectroscopy.

Understanding mass transfer kinetics within individual porous particles is crucial for theoretically explaining the retention and elution behaviors in chromatography and drug delivery. Using laser trapping and fluorescence microspectroscopy, we investigated the diffusion mechanism of coumarin 102 (C102) into single octadecylsilyl particle in acetonitrile (ACN)/water, N,N-dimethylformamide (DMF)/water, and 1-butanol (BuOH)/water solutions. The intraparticle diffusion behavior of C102 was evaluated using the spherical diffusion equation, allowing us to determine the intraparticle diffusion coefficients (Dintra): (8-10) × 10-9 cm2 s-1 for ACN, (10-16) × 10-9 cm2 s-1 for DMF, and (4-6) × 10-9 cm2 s-1 for BuOH. The obtained Dintra values were further analyzed using a pore and surface diffusion model. Thus, we revealed that the diffusion mechanism of C102 differed depending on the organic solvent: surface diffusion for ACN and DMF and pore and surface diffusions for BuOH were observed. This difference is attributed to the formation of a concentrated liquid phase of ACN and DMF at the interface of the alkyl chain and the bulk solution in the pore.

Optical maneuvering of photofunctioning hybrid perovskite for future photonics potential application

Hybrid perovskites, known for their multidimensional opto-electronic properties and capability to generate multi-color emission bands, are highly sought after for light-emitting devices due to their liquid crystalline behavior in the solution phase. This behavior enables easier ion mobility, facilitating ion exchange processes. Exploiting the liquid crystalline properties of perovskites, we applied a halogen exchange reaction in the solution phase using tightly focused laser trapping techniques. Our study demonstrates the successful development of high-luminescent bulk single crystals of methylammonium lead halide alloy MAPb(Br/I)3, which exhibit multi-color emission capabilities, underscoring their potential for optoelectronic applications. We fabricated bulk single crystals of MAPbBr3, measuring 20 × 20 μm2, displaying bright green luminescence. Using a tightly focused trapping laser beam (1064 nm, 1 Watt) with a high numerical aperture (0.9, 60X objective lens), we irradiated a specific area of the green luminescent MAPbBr3 single crystal immersed in a 200 μM methylammonium iodide (MAI) solution for 30 min at room temperature. This treatment induced a red emission band at 605 nm at the irradiated location, while surrounding areas retained the original green luminescence. In a parallel experiment, increasing the iodide concentration to 300 μM caused the emission color of a similar-sized crystal to change uniformly from green to red. Photoluminescence spectroscopy and EDS confirmed the halide exchange from MAPbBr3 to MAPbBr2.3I0.7. This method, influenced by laser parameters such as power, wavelength, and aperture, provides new opportunities for multi-color emission patterning and offers significant insights for advancing optoelectronics.

In-situ observation of DL-alanine crystallization from a laser-trapped dense liquid droplet as a heterogeneous nucleation site

Abstract Nucleation from an aqueous solution is an important step in crystallization which controls the physicochemical properties of crystalline materials. Although dense liquid droplets are considered as a precursor of a crystal in the two-step nucleation model, their actual role is unclear. Our in-situ microscopic observations of the crystallization of DL-alanine from a dense liquid droplet trapped by laser tweezers show that liquid droplets play the role of a substrate, facilitating heterogeneous nucleation, rather than a precursor of a crystal.

One must reconstitute the functions of interest from purified proteins.

I am often asked by students and younger colleagues and now by the editors of this issue to tell the history of the development of the in vitro motility assay and the dual-beam single-molecule laser trap assay for myosin-driven actin filament movement, used widely as key assays for understanding how both muscle and nonmuscle myosin molecular motors work. As for all discoveries, the history of the development of the myosin assays involves many people who are not authors of the final publications, but without whom the assays would not have been developed as they are. Also, early experiences shape how one develops ideas and experiments, and influence future discoveries in major ways. I am pleased here to trace my own path and acknowledge the many individuals involved and my early science experiences that led to the work I and my students, postdoctoral fellows, and sabbatical visitors did to develop these assays. Mentors are too often overlooked in historical descriptions of discoveries, and my story starts with those who mentored me.

Manipulation of large, irregular-shape particles using contour-tracking optical tweezers.

While the optical tweezers technique is a promising tool for manipulation of microparticles, its application to large (>50 µm) particles and irregular-shape ones is still a hard task. In this Letter, we propose what is to our knowledge a novel concept of contour-tracking optical tweezers (CTOTs), which extract the contour of the objective particle to form the illumination pattern of the trapping laser into the contour shape in real time. We demonstrated the trapping of polystyrene particles of irregular shape with the size of over 100 µm with CTOTs. Our approach has potential to open the way for expanding the applicability of optical tweezers by enabling manipulation of a variety of samples.

Tightly Trapped Atom Interferometer inside a Hollow-Core Fiber

We demonstrate a fiber-guided atom interferometer in a far-off-resonant trap (FORT) of 100 μK. The differential light shift (DLS) introduced by the FORT leads to the inhomogeneous dephasing of the tightly trapped atoms inside a hollow-core fiber. The DLS-induced dephasing is greatly suppressed in π/2-π-π/2 Doppler-insensitive interferometry. The spin coherence time is extended to 13.4 ms by optimizing the coupling of the trapping laser beam into a quasi-single-mode hollow-core anti-resonant fiber. The Doppler-sensitive interferometry shows a much shorter coherence time, indicating that the main limits to our fiber-guided atom interferometer are the wide axial velocity distribution and the irregular modes of the Raman laser beams inside the fiber. This work paves the way for portable and miniaturized quantum devices, which have advantages for inertial sensing at arbitrary orientations and in dynamic environments.

Single-cell analysis reveals microbial spore responses to sodium hypochlorite.

Pollution from toxic spores has caused us a lot of problems because spores are extremely resistant and can survive most disinfectants. Therefore, the detection of spore response to disinfectant is of great significance for the development of effective decontamination strategies. In this work, we investigated the effect of 0.5% sodium hypochlorite on the molecular and morphological properties of single spores of Bacillus subtilis using single-cell techniques. Laser tweezers Raman spectroscopy showed that sodium hypochlorite resulted in Ca2+-dipicolinic acid release and nucleic acid denaturation. Atomic force microscopy showed that the surface of treated spores changed from rough to smooth, protein shells were degraded at 10 min, and the permeability barrier was destroyed at 15 min. The spore volume decreased gradually over time. Live-cell imaging showed that the germination and growth rates decreased with increasing treatment time. These results provide new insight into the response of spores to sodium hypochlorite.