This paper reports an experimental investigation of the characteristics of bubble chains and their induced flow fields in a quiescent fluid at gas flow rates of 5, 10, 50, and 100 ml/min. The shadowgraphy and laser-induced-fluorescence particle image velocimetry (LIF-PIV) were used to analyze quantitatively the bubble morphology, kinematics, and induced flow fields. The results show that with increasing gas flow rate, the bubbles exhibit a larger average diameter, aspect ratio, and more-abundant morphology. The bubble motion includes a rectilinear rise followed by an oscillating rise, and the position where the bubble deviates from the linear trajectory is associated with the point of maximum bubble aspect ratio. The amplitude of bubble oscillation and the position of the instability both increase with the gas flow rate, except for the case of 50 ml/min. The bubble-induced flow field at higher gas flow rates exhibits stronger entrainment, which reflects the ability of the flow to entrain the surrounding fluid. The instantaneous flow-field results show that bubble-induced wake shedding is the main cause of the change in bubble rise state and trajectory instability. The direction of bubble deflection is related directly to the sequence of wake shedding: when the clockwise (resp. counterclockwise) vortex in the wake is the first to shed, the bubble deflects to the left (resp. right). The study also reveals that the simultaneous shedding of counterclockwise and clockwise vortices at 50 ml/min results in the bubbles continuing to rise rectilinearly for a period of time, and the position of trajectory destabilization has a maximum height.