Free space optical communication is developing towards laser communication networking. A novel method of quadrant detector (QD) multi-spot position detection based on orthogonal frequency division multiplexing (OFDM) is proposed for multi-spot laser communication systems. The mathematical model is constructed, and the Monte Carlo method is used to validate it. The position calculation of three beams incident on QD is simulated. The influence of key parameters on the accuracy of position detection is analyzed when the spots are at the same position and a different position. The results show that when the SNR of the system is 31.74 dB, the radius of the Gauss spot is 2 mm, the number of FFT (fast Fourier transform) points is 1024, and the center of the multi-spot is at the same position of the detector target; the accuracy of signal position detection calculated by the equation is 1.433 μm, and the simulation results are 1.351 μm, 1.354 μm, and 1.389 μm, respectively. When the center position of the multi-spot is at different positions of the detector target, the detection accuracy calculated by the formula is 1.438 μm, 1.433 μm, and 1.434 μm, respectively, and the simulation results are 1.419 μm, 1.387 μm, and 1.346 μm, respectively. The experimental results verify the effectiveness of the proposed multi-target simultaneous detection method. This article proposes a new multi-spot position detection method which can not only achieve one-to-multiple node laser communication but also improve the accuracy of point position detection.