Laser Biostimulation Research Articles

Adjunctive use of laser biostimulation with nonsurgical periodontal therapy: a split-mouth, randomized, case-control study in diabetic and nondiabetic periodontitis patients.

Laser biostimulation therapy (LBT) is suggested to have positive effects on periodontal healing. This study evaluated LBT with nonsurgical periodontal therapy (NSPT) in diabetes mellitus (DM) and systemic health (SH) conditions. Thirty periodontitis patients (15 with DM and 15 with SH) were included in the study, which had a split-mouth design, by applying LBT in the mouth of the same systemic condition. Thus, 4 study groups were formed, as 1) NSPT - DM: NSPT alone in DM, 2) NSPT + LBT - DM: NSPT + LBT application in DM, 3) NSPT - SH: NSPT alone in SH, and 4) NSPT + LBT - SH: NSPT + LBT application in SH. NSPT was performed on days 15, 30, 37, 44, 51, 58, and 65. LBT was performed 6 times on days 30, 37, 44, 51, 58, and 65 with an Nd:YAG laser. The plaque index (PI), gingival index (GI), bleeding on probing (BOP), probing pocket depth (PPD), and clinical attachment level (CAL) were assessed as the clinical parameters and recorded at baseline and days 30, 37, and 72. Gingival crevicular fluid levels of interleukin 1 beta (IL-1β) and IL-10 were evaluated by ELISA as the biochemical parameters at baseline and on days 30, 37, and 72. Clinical parameters had improved in all of the groups on day 72 (p < 0.01). PPD and CAL improved more in the DM group with NSPT and LBT group than in the DM group with NSPT without LBT on day 37 (p < 0.05). IL-1β decreased and IL-10 increased in all of the groups on day 72 (p < 0.01). This change was more evident in the DM group with NSPT and LBT than in the DM group with NSPT without LBT on day 7 (p < 0.05). These results revealed the short-term impacts of LBT on periodontal healing, which return to ineffectiveness with repeated irradiation. Therefore, it may be speculated that LBT via the protocol herein may have a short-term antiinflammatory contribution to NSPT, only in impaired healing conditions such as DM.

Photo biomodulation of dental derived stem cells to ameliorate regenerative capacity: In vitro study

BackgroundDental regeneration benefits from improving the features of dental derived stem cells. Gallium-aluminum-arsenide laser had a significant role in modification of cell behavior in different cell lines and culture conditions. Hence, exploring its mechanism and effect on dental derived stem cells would benefit prospective regenerative dental therapies. ObjectivesTo assess the impact of photo biomodulation by Low-Level-Laser on isolated Dental Pulp derived Stem Cells and Periodontal Ligament derived Stem Cells regarding their proliferation and osteogenic differentiation. MethodsIsolated DPSCs and PDLSCs from impacted third molars were subjected to Gallium-aluminum-arsenide laser for 12 sec and 3.6 J/cm2. The proliferative capacity was evaluated via 3-(4,5-dimethylthiazol-2-yl),2,5-diphenyltetrazolium bromide (MTT) Assay and Trypan blue stain. Cell osteogenic differentiation potentials were assessed by alkaline phosphatase assay and alizarin red stain, polymerase chain reaction was performed to quantify Nuclear factor Kappa gene expression. ResultsDPSCs subjected to laser bio-stimulation showed the best results regarding cell viability (MTT) and osteogenic differentiation (ALP assay), and calcium deposition at 3 intervals (3, 7, 14 days), meanwhile, PDLSCs subjected to laser bio-stimulation showed better result than control but less than DPSCs. While NF-KB gene expression was proven to be approximately comparable for both groups. Generally, the Photo-bio modulated groups showed better results than their control groups. ConclusionLow-level laser bio-stimulation (LLL) therapy improves DPSC and PDLSC osteogenic differentiation and proliferation via the activation of the NF-KB pathway. Also, the DPSCs outperformed PDLSCs in terms of performance. Clinical significanceThese results can be beneficial information and a reference database for more research in tissue engineering, dental therapy, and regeneration.

Laser Biostimulation Induces Wound Healing-Promoter β2-Defensin Expression in Human Keratinocytes via Oxidative Stress.

The innate immune system is the first line of defense of the body composed of anatomical barriers, such as skin and mucosa, as well as effector cells, antimicrobial peptides, soluble mediators, and cell receptors able to detect and destroy viruses and bacteria and to sense trauma and wounds to initiate repair. The human β-defensins belong to a family of antimicrobial small cationic peptides produced by epithelial cells, and show immunomodulatory and pro-healing activities. Laser biostimulation is a therapy widely used to contrast microbial infection and to accelerate wound healing through biological mechanisms that include the creation of oxidative stress. In this paper, we explored laser biostimulation's ability to modulate the production of two β-defensins, hBD-1 and hBD-2, in human keratinocytes and whether this modulation was, at least in part, oxidative-stress-dependent. Human spontaneously immortalized keratinocytes (HaCaT) were stimulated using laser irradiation at a 980 nm wavelength, setting the power output to 1 W (649.35 mW/cm2) in the continuous mode. Cells were irradiated for 0 (negative control), 5, 10, 25 and 50 s, corresponding to an energy stimulation of 0, 5, 10, 25 and 50 J. Positive control cells were treated with lipopolysaccharide (LPS, 200 ng/mL). After 6 and 24 h of treatment, the cell conditioned medium was collected and analyzed via ELISA assay for the production of hBD-1 and hBD-2. In another set of experiments, HaCaT were pre-incubated for 45 min with antioxidant drugs-vitamin C (Vit. C, 100 µM), sodium azide (NaN3, 1 mM); ω-nitro-L-arginine methyl ester (L-NAME, 10 mM) and sodium pyruvate (NaPyr, 100 µM)-and then biostimulated for 0 or 50 s. After 6 h, the conditioned medium was collected and used for the ELISA analysis. The hBD-1 and hBD-2 production by HaCaT was significantly increased by single laser biostimulation after 6 h in an energy-dependent fashion compared to basal levels, and both reached production levels induced by LPS. After 24 h, only hBD-2 production induced by laser biostimulation was further increased, while the basal and stimulated hBD-1 levels were comparable. Pre-incubation with antioxidative drugs was able to completely abrogate the laser-induced production of both hBD-1 and hBD-2 after 6 h, with the exception of hBD-1 production in samples stimulated after NaN3 pre-incubation. A single laser biostimulation induced the oxidative-stress-dependent production of both hBD-1 and hBD-2 in human keratinocytes. In particular, the pro-healing hBD-2 level was almost three times higher than the baseline level and lasted for 24 h. These findings increase our knowledge about the positive effects of laser biostimulation on wound healing.

The Endocannabinoid System a Turning Point in Optimizing Physiotherapy Procedures in Knee Osteoarthritis

Introduction: Knee Osteoarthritis (KOA) is characterized by wear, tear, and it's a slowly progressive loss of cartilage, that becomes finally disabling. KOA is one of the most analyzed diseases by many medical specialties such as rheumatology, orthopedics, rehabilitation medicine, and physiotherapy. Major symptoms of KOA such as pain, dysfunction, and chronic low-grade inflammation will decrease the quality of life and eventually lead to locomotor disability. Since there are no effective ways to limit KOA progression, involvement of the endocannabinoid system (ECS) may be a non-pharmacological therapeutic alternative in the management of this disease. This study debate aspects of ways to modulate the ECS in KOA, using physiotherapeutic (PT) means such as TENS electroanalgesia, LASER biostimulation, and physical exercises with analgesic effects. Materials and Methods: In the study, we included 82 sources, with the following keywords in the title: knee osteoarthritis, cannabidiol, endocannabinoid, inflammatory pain, analgesia, neuropathic pain, physiotherapy, electrotherapy, and LASER. To perform this review, we searched for the most relevant articles in the field of medicine and physiotherapy in 7 international databases applying inclusion and exclusion criteria. Results: The application of TENS currents in certain doses and frequencies together with LASER biostimulation stimulates the production of endocannabinoids thus controlling pain, and stimulating the ECS. Physical exercise has an antioxidant, and anti-inflammatory role and stimulates the release of endogenous opioids. Conclusions: The results obtained from this meta-analysis may contribute to paradigm shifts in clinical practice related to the treatment of pain by PT. TENS, LASER, and physical exercise are effective clinical tool that limits chronic inflammation and pain by involving the ECS. More studies are needed to understand endocannabinoid system involvements in KOA, and that will inspire medical doctors and physiotherapists to improve long-term treatment strategies.

The Effect of Hyrax-Type Rapid Maxillary Expansion with or without Laser Biostimulation on Mid Palatal Suture in Children with Posterior Crossbite

Purpose: The aim of the following study was to evaluate the use of low-level laser application on the width of the Mid palatal suture (MPS) after rapid maxillary expansion. Materials and methods: patients age range 10 ± 2.5 years were prospectively selected from outpatient clinic of the Orthodontic Department, Faculty of Dental Medicine for Girls, Al-Azhar University (Girls’ Branch). Each patient was examined, full diagnostic records and cone beam computed tomography (CBCT) were taken before appliance cementation (T0), after six months and at the end of retention phase (T1). The hyrax appliance was used for rapid maxillary expansion (RME). The activation protocol of the expansion screw was 2 full turns daily. The laser type used was the e Epic™ X diode laser system, 940 nm wavelength, 40 mW power, and 10 J/cm2 density. Results: No significant differences were found in the two areas between the control and the laser group. Conclusions: The mid palatal suture width showed no statistical significant differences between the control and laser group in anterior and posterior region.

The Effect of Hyrax-Type Rapid Maxillary Expansion with or without Laser Biostimulation on Mid Palatal Suture in Children with Posterior Crossbite

Purpose: The aim of the following study was to evaluate the use of low-level laser application on the width of the Mid palatal suture (MPS) after rapid maxillary expansion. Materials and methods: patients age range 10 ± 2.5 years were prospectively selected from outpatient clinic of the Orthodontic Department, Faculty of Dental Medicine for Girls, Al-Azhar University (Girls’ Branch). Each patient was examined, full diagnostic records and cone beam computed tomography (CBCT) were taken before appliance cementation (T0), after six months and at the end of retention phase (T1). The hyrax appliance was used for rapid maxillary expansion (RME). The activation protocol of the expansion screw was 2 full turns daily. The laser type used was the e Epic™ X diode laser system, 940 nm wavelength, 40 mW power, and 10 J/cm2 density. Results: No significant differences were found in the two areas between the control and the laser group. Conclusions: The mid palatal suture width showed no statistical significant differences between the control and laser group in anterior and posterior region.

Transverse Skeletal Effects of Hyrax-Type Rapid Maxillary Expansion with or without Laser Biostimulation

Purpose: The aim of this study is to assess the effect of Laser biostimulation on the transverse changes of maxilla and mandible after rapid maxillary expansion in growing patients. Subject and Methods: Twenty growing patients with posterior teeth in crossbite were included in this study. Patients were randomly divided into two groups. Rapid maxillary expansion had been carried out for all patients. In laser group Laser was applies on mid palatal suture during active expansion and during retention. The control group had no laser with their expansion procedures. Pre-treatment and post-treatment Cone beam computed tomographies were used to compare the changes in skeletal maxillary and mandibular widths. Results: The two groups showed insignificant increase in both interjugale distance and bigonial distance. No significant difference was found between the laser group and the control group regarding the amount of change of both bigonial and inter widths. P ≤ 0.05. Conclusions: In both Laser and non-Laser groups, Rapid maxillary expansion in growing patients with crossbite produces insignificant increase in maxillary transverse inter jugale and bigonial widths. Laser application during maxillary expansion has statistically insignificant effect on changes in maxillary interjugale width and mandibular bigonial width.

Transverse Skeletal Effects of Hyrax-Type Rapid Maxillary Expansion with or without Laser Biostimulation

Purpose: The aim of this study is to assess the effect of Laser biostimulation on the transverse changes of maxilla and mandible after rapid maxillary expansion in growing patients. Subject and Methods: Twenty growing patients with posterior teeth in crossbite were included in this study. Patients were randomly divided into two groups. Rapid maxillary expansion had been carried out for all patients. In laser group Laser was applies on mid palatal suture during active expansion and during retention. The control group had no laser with their expansion procedures. Pre-treatment and post-treatment Cone beam computed tomographies were used to compare the changes in skeletal maxillary and mandibular widths. Results: The two groups showed insignificant increase in both interjugale distance and bigonial distance. No significant difference was found between the laser group and the control group regarding the amount of change of both bigonial and inter widths. P ≤ 0.05. Conclusions: In both Laser and non-Laser groups, Rapid maxillary expansion in growing patients with crossbite produces insignificant increase in maxillary transverse inter jugale and bigonial widths. Laser application during maxillary expansion has statistically insignificant effect on changes in maxillary interjugale width and mandibular bigonial width.

Clinical image: A particular lingual hematoma in an 8 year – old male patient

We present a case of an 8-year-old boy with an atypical hematoma on the lateral side of the tongue. The lesion occurred due to a trauma that happened while he was playing at school. The child did not remember to tell anything to teachers or parents until the hematoma and together with it the pain appeared. Due to the extension of the lesion the parents were really worried about, so they brought to us in urgency the child in order to visit him. After a long conversation with both parents and child, we were able to understand the traumatic nature of the lesion, and to rule out more severe diseases. We proposed LASER biostimulation and LASER photocoagulation to reduce the inflammation state of the lingual tissue and so the pain.

Evaluation of the efficacy of laser biostimulation and intralesional triamcinolone acetonide in OSMF: A clinical study

Evaluation of the efficacy of laser biostimulation and intralesional triamcinolone acetonide in OSMF: A clinical study

Effect of Low-Level Diode Laser Bio-Stimulation on Implant Stability Before, During and After Immediate Implants in Mandibular Molars. (A Randomized Clinical Study)

Aim: Tooth loss can affect the person’s life dramatically. Tooth loss is accompanied by bone loss in all dimensions, so preservation of the bone is mandatory for placement of dental implant. Immediate implant can preserve alveolar bone. Due to difference in shape between the implants and extracted roots a gap appears between the immediately placed implant and the extraction socket resulting in jumping distance, Bone grafts are commonly used to fill this gap, recent studies suggest that jumping gaps shouldn’t always be grafted. Low level bio stimulation has stimulatory effect on bone cells so it can be used in immediate implants to increase bone formation around the implants. Materials and methods: this study was carried in the hospital of future university. A total of twelve patients with non-restorable mandibular molar participated in this study. A total of twenty implants were placed immediately in mandibular molar region without bone grafting, thew were divided equally into two groups. Surgical technique was similar for both groups. After immediate implant placement with primary stability of 35 N, both groups received custom made healing collar using flowable composite to seal the jumping distance.The study group (intervention) received a total of 60J/cm2 .10J/cm2 3 days before extraction, another 10J/cm2 at the fresh socket, another 10 J/cm2 after osteotomy, another 10J/cm2 after implant placement, another 20J/cm2 at the follow up visit with 3 days interval. After six weeks secondary implant stability was measured for both groups using Resonance Frequency Analysis device (RFA). Prosthetic phases started for implants that showed sufficient secondary stability. Results: All implants enrolled in this study showed sufficient secondary stability except for one totally avulsed implant from the control group. Study group showed more stability upon measuring using RFA device after six weeks of implant placement. The study group has shown a higher secondary stability compared to control group, the mean ISQ value of study (71.89±2.67) was significantly higher than control (62.43±8.62). Conclusion: Based on the results of our study we concluded that low level diode laser 980nm has a stimulatory effect on bone formation when applied before, during and after immediate implant placement, According to this study 60J/cm2 is recommended dose and it also gave better soft tissue healing results

An Unusual Complication of Root Canal Treatment and its treatment by Laser Biostimulation

An Unusual Complication of Root Canal Treatment and its treatment by Laser Biostimulation

Effect of Laser Biostimulation on Germination of Sub-Optimally Stored Flaxseeds (Linum usitatissimum)

Sub-optimal storage of grains could deteriorate seed germination and plant viability. Recent research studies have established that laser biostimulation of seeds could be used as a safe and sustainable alternative to chemical treatment for improving crop germination and growth. Herein, the efficacy of this novel technique is evaluated to see if poor germinability caused by sub-optimal storage of flaxseeds (Linum usitatissimum) could be reversed using laser biostimulation. Healthy flaxseeds were first subjected to sub-optimal storage conditions (30 °C for ten weeks) to degrade their germinability. Two low-cost lasers, including a single-wavelength red laser (659 nm) and a dual-wavelength green/infrared laser (531 and 810 nm (ratio ~10:1)) were then used on two groups viz. healthy (properly stored) and sub-optimally stored (artificially degraded (AD)) seeds and irradiated for 0 (control), 5, 10, and 15 min using total power densities of 7.8 and 6.2 mW/cm2, respectively. In the case of AD seeds, 5-min dual-wavelength laser treatment was found to be the most efficient setting as it improved the mean germination percentage, mean germination time, germination speed, germination rate index, wet weight, and dry weight by 29.3, 16.8, 24.2, 24.2, 15.7, and 20.6%, respectively, with respect to control samples. In the case of healthy seeds, dual-wavelength laser treatment could induce significant enhancement in seeds’ root length, wet weight, and dry weight (improved by 26, 23, and 8%, respectively) under 10 min of irradiation. On the other hand, the effect of applied red laser treatment was not very promising as it could only induce significant enhancement in the mean germination time of AD seeds (improved by 17%). Overall, this study demonstrates the potential of laser biostimulation in reversing the adverse effect of poor crop storage. We believe these findings could spur the development of a physical tool for manipulating seed germination and plant growth.

Effect of laser bio-stimulation with a wavelength of 632.8 on children with spastic cerebral palsy

Cerebral Palsy (CP) defined as lasting clutters gather of development and posture advancement which is the cause of activity confinement that are credited to non-dynamic unsettling influences which happened in the developing fetal or newborn child brain. Concurring to European information, the average recurrence of CP is 2.08 per 1000 live births. Study proposed to confirm low laser acupuncture impact with wavelength 632.8 nm. on immunity improvement in children with spastic CP. This study was conducted on 40 children with spastic CP both sexes from 1 to 3 years old. They were chosen from the outpatient clinic of pediatric in the National Institute of Laser Enhanced Sciences (NILES) and physical therapy outpatient clinic at Benha Teaching Hospital. They were divided randomly into two groups equal in number: Group A: (Control group) received physiotherapy session only lasted for an hour for 3 sessions / week for 12 weeks. Group B: (study group): received laser acupuncture with wavelength 632.8 nm and power output: 50 mw for 3 sessions / week for 12 weeks as well previous program of physical therapy.

Effect of Laser Bio stimulation and Bone Graft in Treatment of a Large Periapical Lesion – A CBCT Analysis

India accounts for 23% of global TB burden. Of these 38% are of Extra Pulmonary Tuberculosis. Lymph node TB is the most common EPTB, pleural effusion is second most common type of EPTB in India. The bacteriological confirmation to diagnose EPTB is more difficult due to its paucibacillary nature. This study aims assessing the comparison of ADA with Gene- Xpert MTB RIF in pleural fluid in the diagnosis of tubercular pleural effusion. Study was a cross-sectional study carried out at the Department of Respiratory Medicine and Department of Internal Medicine, Muzaffarnagar Medical College, and School of Medical Science and Research, Greater Noida, Gautam Budh Nagar, UP from January 2018 to March 2019. Paired and unpaired Student's t-test, Chi square test, P &lt; 0.05 was considered statistically significant; SPSS 18.0 software was used for analysis. The detection of MTB and rifampicin resistance using the Gene-Xpert MTB/RIF with pleural fluid ADA assay was assessed in 66 specimens. With ADA cut off as 40IU/L, 60 cases had an ADA &gt; 40IU/L and 6 cases had ADA &lt; 40IU/L. Pleural fluid Gene-Xpert detected MTb in 22 samples while in 44 samples MTB not detected. All pleural fluid MTb detected by Gene-Xpert were rifampicin sensitive. Gene-Xpert detected 2 tubercular pleural effusion in which ADA level was less than 40. Study shows that there was no statistically significance in diagnostic value between Gene- Xpert and ADA pleural fluid however Gene-Xpert MTB/RIF is useful method for rapid detection of MTB and Rifampicin resistance simultaneously. Estimation of ADA in pleural fluid is rapid inexpensive laboratory investigation where the diagnosis is uncertain. We found no significant connection between CBNAAT positivity and ADA level. In developing country lymphocyte predominant effusions with high ADA levels (&gt; 40 U/L) is better option but definitely combined use of ADA and Gene-Xpert is better than using individually for the diagnosis of Tubercular pleural effusion

Induction of Ovulation using Low-Level Laser Biostimulation

Abstract&#x0D; Aim: Low level laser therapy (LLLT) has been used in biostimulation of rat ovaries using a fibreoptic laser introduced directly through the vagina to deliver laser doses, which is a new modality of laser induction of ovulation, compared with conventional medical induction of ovulation using clomiphene citrate and with non-interventional controls. &#x0D; Study design: A Prospective experimental study carried out from January 2014 to February 2016.&#x0D; Setting: University-based photobiology laboratory.&#x0D; Materials and methods: Seventy-two (72) Female-Wistar Albino rats were used in this study, divided into three groups: 17 rats used as a control group, 19 rats received clomiphene citrate (Clomid group) and 36 rats exposed to diode laser 660 nm wavelength (laser group). Histopathological assessment of rat ovaries was done in the three study Groups.&#x0D; Results: Higher number of follicles was found in laser group compared to controls, the difference was significant in the Primordial follicles. Non-significant increase in the number of Primordial follicles in laser group was found compared to the clomid group. Clomid group showed a non-significant increase in primary, secondary and Antral follicles.&#x0D; Conclusion: This study shows that ovarian laser biostimulation is a new encouraging method for induction of ovulation.

Effect of laser biostimulation and a low-calorie diet vs. a low-calorie diet alone on insulin resistance, inflammatory biomarkers, and depression among obese postmenopausal women: a randomized controlled trial.

Postmenopausal women are significantly predisposed to a multitude of disorders. Laser biostimulation (LB) and a healthy diet have been linked to multiple health benefits. This study aimed to investigate the effect of a combined LB and balanced low-calorie diet (LCD) vs. an LCD alone on insulin resistance (IR), inflammatory biomarkers, and depression score in obese postmenopausal women. In the present study, a total of 66 postmenopausal women, with a mean age of 66.61 ± 4.80 years and a body mass index (BMI) of 35.93 ± 2.67 kg/m2, were randomized into two equal groups. The experimental group received LB and LCD (including 50-60% carbohydrates, 15-20% protein, 20-35% fat, and 25 g of fiber/day plus a restriction of 500-1000 kcal/d), while the control group followed the same diet program only for 12 weeks. Before and after the intervention, IR [measured by the Homeostatic Model Assessment of Insulin Resistance (HOMA-IR index)], inflammatory biomarkers [C-reactive protein (CRP), white blood cells (WBCs), lymphocytes, and erythrocyte sedimentation rate (ESR)], and depression level [as assessed by the Hamilton Depression Rating Scale (HAMD-17)] were all measured. Using an intention-to-treat analysis for 60 women who completed the study, the body weight average reduction was -13.14% for the experimental group (p<0.001) vs. -6.36% for the control group (p<0.001). BMI, IR, inflammatory markers, and depression levels were also similarly changed. In postmenopausal obese women, adding LB to a suitable dietary program provides the most significant benefit in terms of lowering IR, metabolic inflammation, and depression.

Effect of Laser Bio stimulation and Bone Graft in Treatment of a Large Periapical Lesion – A CBCT Analysis

Nonsurgical endodontic treatment is a definitive predictable treatment option in most cases, but endodontic surgery may be indicated for teeth with persistent peri apical pathosis unresponsive to non-surgical approaches. This paper reports the one case of bone healing after peri apical surgery using laser biostimulation and hydroxyapatite bone graft assessed using CBCT analysis. Each case was evaluated using Pre-operative CBCT. Post treatment evaluation was done clinically, radiographically and by CBCT analysis.

Effect of Laser Biostimulation on Germination of Wheat

HighlightsThe effect of laser biostimulation on wheat germination was explored using a single- and dual-wavelength (DW) laser.An 80 mW green-infrared DW laser treatment significantly enhanced several germination traits of wheat.The effect of a 100 mW single-wavelength red laser on wheat germination was non-significant.Abstract. Laser biostimulation of seeds has established itself as a safe and sustainable alternative to genetic modification or chemical use to enhance plant germination and growth. A knowledge gap, however, exists to define optimal laser parameters for different seeds as inappropriate irradiation may seriously damage or destroy germinability. To this end, the effect of laser biostimulation on germination of Canada Western Red Winter (CWRW) wheat seeds was evaluated using two low power portable lasers: 1) a single-wavelength red laser (659 nm) and 2) a dual-wavelength (DW) green/infrared laser [531 and 810 nm (ratio ~10:1)]. The seeds were pretreated with laser light before germination tests for 5, 10, and 15 minutes using total power densities of ~14.2 and 11.3 mW/cm2, respectively. Laser treatment with a DW laser for a duration of 10 min was found to be the most efficient as it significantly enhanced mean germination time, germination rate index, germination speed, number of roots, and hypocotyl length by 14.3%, 15.2%, 15.2%, 31.8%, and 60.9%, respectively, with respect to control samples. The effect of single-wavelength red laser on CWRW wheat seed germination traits was not statistically significant. To the best of our knowledge, this study is the first on evaluating the effect of DW laser treatments in plant biostimulation and introduces a new pathway for manipulating the germination, growth, and development of seeds/plants. Keywords: Agriculture, Biostimulation, Dual-wavelength laser, Laser, Wheat.

Long COVID Oral Cavity Symptoms Based on Selected Clinical Cases.

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is characterized by variable clinical features, different durations, and several previously unheard-of late complications. Knowledge about this infection is constantly evolving.The aim of the study is to present selected cases of the most common symptoms of long COVID in the oral cavity.Among the 1,256 studied patients, 32% of them had discoloration, ulceration, and hemorrhagic changes on the oral mucosa, 29.69% had mycosis located on the tongue, 25.79% of patients had aphthous-like lesions on the hard palate, and in 12.5% atrophic cheilitis was observed. During the anamnesis, approximately 60% of patients reported salivary secretory disorders in the initial period of infection, which is 6.68% prolonged up to 4 months after systemic symptoms disappeared. In an extreme case, an aphthous-like lesion was located on the hard palate, which persisted for 6 months. Approximately 36% of patients did not agree to the proposed treatment. As a result, they only received recommendations on the use of oral hygiene products and received weekly check-ups. In this group of patients, most pathological changes spontaneously cleared after 3 weeks. The elderly with coexisting diseases, persons with a more severe SARS-CoV-2, and hospitalized patients had more extensive and severe lesions in the oral cavity that persisted for a long time after infection.In patients after the SARS-CoV-2 infection and suspected of this infection, a detailed intraoral examination should be performed, and the patient must be obligatorily monitored for a minimum period of 6 months. Depending on the patient's clinical condition, changes in the oral cavity require observation, basic or specialist treatment. In the case of changes in the cavity without pain symptoms, observation should be made for approximately 4 weeks and wait for the spontaneous regression of the changes. However, when pain occurs, a good solution is to use laser biostimulation. In the case of complex pathological changes occurring in the oral cavity, the patient should be directed for specialist treatment.