Larval Host Plant Research Articles

First record of the genus Zygaenoprocris Hampson, 1900 (Lepidoptera: Zygaenidae) from Türkiye (Turkey)

Zygaenoprocris persepolis (Alberti, 1938) is found in eastern Türkiye (Turkey). This is the first record of the genus Zygaenoprocris Hampson, 1900 (Lepidoptera: Zygaenidae) from Türkiye. The larval host-plant of Z. persepolis in Türkiye is Atraphaxis spinosa L.

Rapid Evolution of Host Repertoire and Geographic Range in a Young and Diverse Genus of Montane Butterflies.

Evolutionary changes in geographic distribution and larval host plants may promote the rapid diversification of montane insects, but this scenario has been rarely investigated. We studied rapid radiation of the butterfly genus Colias, which has diversified in mountain ecosystems in Eurasia, Africa, and the Americas. Based on a dataset of 150 nuclear protein-coding genetic loci and mitochondrial genomes, we constructed a time-calibrated phylogenetic tree of Colias species with broad taxon sampling. We then inferred their ancestral geographic ranges, historical diversification rates, and the evolution of host use. We found that the most recent common ancestor of Colias was likely geographically widespread and originated ~3.5 Ma. The group subsequently diversified in different regions across the world, often in tandem with geographic expansion events. No aspect of elevation was found to have a direct effect on diversification. The genus underwent a burst of diversification soon after the divergence of the Neotropical lineage, followed by an exponential decline in diversification rate toward the present. The ancestral host repertoire included the legume genera Astragalus and Trifolium but later expanded to include a wide range of Fabaceae genera and plants in more distantly related families, punctuated with periods of host range expansion and contraction. We suggest that the widespread distribution of the ancestor of all extant Colias lineages set the stage for diversification by isolation of populations that locally adapted to the various different environments they encountered, including different host plants. In this scenario, elevation is not the main driver but might have accelerated diversification by isolating populations.

Understanding life cycle and identification of larval host plant of lesserknown butterfly Truncate Imperial Cheritrella truncipennis from Sikkim Himalaya, India

Understanding life cycle and identification of larval host plant of lesserknown butterfly Truncate Imperial Cheritrella truncipennis from Sikkim Himalaya, India

A new species of Acossus Dyar (Lepidoptera: Cossidae) establishes diurnal flight in New World carpenter-moths

Synthetic pheromones formulated for Acossus centerensis Lintner, 1877 led to the discovery of a new taxonomically cryptic species, Acossus boreocryptus Schmidt & Jaeger sp. nov. Adult males were active only during the daytime, exceptional among cossid moths, and the first documented case of diurnality in New World Cossidae. Males were attracted to lures baited with (E,E) 3,5-tetradecadienyl acetate (EE-TDDA). The larval host plant of A. boreocryptus is trembling aspen, Populus tremuloides Michx., but the immature stages remain undescribed. Acossus boreocryptus is the smallest species in the genus, distinguished from its sister species A. centerensis by adult morphology, mtDNA barcode, male diurnal activity, and seasonal phenology.

Integrative taxonomy of Teucriogethes pollen beetles (Coleoptera: Nitidulidae: Meligethinae), with implications on the systematics of the genus Teucrium (Lamiaceae).

In highly specialised phytophagous insects, important insights on phylogeny of the involved insect group can be often inferred from the evolutionary history of their host plants, and vice-versa, as in the case for a new, peculiar species of pollen beetles, recently discovered in Central China (Hubei Province). This new species is described herein as Teucriogethes huangae Liu & Audisio, sp. nov., and information on its ecology and systematic position is provided. The new species, associated as larvae with a strongly isolated member of the genus Teucrium L. (Lamiaceae), T. ornatum Hemsl., exhibiting a problematic taxonomic position, represents in turn an isolated and morphologically rather aberrant taxon in its lineage, probably the largest in size (2.2-2.8mm), and the first endemic representative of its genus in the whole Eastern Palaearctic. An updating of the taxonomic assessment of members of Teucriogethes and on possible evolutionary relationships with their larval host plants, based on an integrative taxonomy approach, is finally presented. A key to identification of all known species is also introduced. ZooBank: urn:lsid:zoobank.org:pub:E349044B-29A5-416C-85CA-56143C7A29BE.

Increasing rate of moth species establishment over 120 years shows no deceleration

Abstract Around the world, the number of species establishing outside of their native range has and continues to increase rapidly. Some of these non‐native species are invasive and can cause negative environmental, economic, and social impacts, including declines in native biodiversity. Species can become established either through natural dispersal or with intentional or inadvertent human assistance, but the relative importance of these two modes over time has been little studied. We compiled data on 116 moth species that became established in Great Britain (GB) between 1900 and 2019. We classed each species as either an immigrant, which had colonised naturally, or an adventive, which had colonised through human assistance, and categorised species as feeding on either native or non‐native larval host plants. We found that the rate of moth species establishment has accelerated steadily, at 21% increase per decade, showing no signs of saturation. Immigrant species showed a steady rate of establishment between 1900 and 1935, followed by a significant acceleration of 11% per decade thereafter, which most closely matches the rate of immigrants that feed on native host plants. Immigrants that feed on non‐native host plants show a slower increase across the whole time period compared to all species, of 13% per decade. Adventive species show a similar rate of increase in establishment to all moth species, at a rate of 26% per decade. Adventives on non‐native host plants show a similar trend, but we find evidence for a post‐1980s increase in the rate of establishment for adventives that feed on native host plants. Our results indicate that the global case for enhanced biosecurity, around both the monitoring of natural colonisations and for the horticultural trade, remains very strong as non‐native colonisations are showing no signs of saturation with ongoing environmental and economic change.

First records of Chamaesphecia efetovi O. Gorbunov, 2019 (Lepidoptera: Sesiidae) in Türkiye (Turkey)

Chamaesphecia efetovi O. Gorbunov, 2019, is recorded from Türkiye for the first time. In 2024 with the help of sex attractants we investigated 11 biotopes with the larval host plant of Ch. efetovi, viz. Marrubium peregrinum L. (Lamiaceae), and found populations of Ch. efetovi in six of them.

A checklist of moths in Bilaspur district, Himachal Pradesh, in the western Himalayan foothills, India

In the first ever enumeration of moth diversity of Bilaspur district, Himachal Pradesh, India, located mostly in the Shivalik range, 82 species/morphospecies were reported, at least 22 of which are new records for Himachal Pradesh, and five are new records for Western Himalayas. In addition to a list of moths for the district supplemented with photographs, identification keys for similar species, larval host plants for species, and a near exhaustive dataset of distribution of the species/genera within and outside India are also provided.

A checklist of butterflies of Sálim Ali Centre for Ornithology and Natural History (SACON) campus, Anaikatty, Western Ghats, India

An updated checklist of butterfly species recorded from the Sálim Ali Centre for Ornithology and Natural History (SACON) campus, constitute a total of 178 species of butterflies, including previous records of 101 species. The butterfly species represent Papilionidae (17), Pieridae (22), Nymphalidae (55), Lycaenidae (54), Riodinidae (1) and Hesperiidae (29). Seventy-seven species are new additions. Among the 178 species, 56 (31.46%) were found to be very common, 74 (41.57%) were moderately common, 42 (23.60%) were rare and 6 (3.37%) as very rare in sightings. Ninety plant species belonging to 33 families as larval host plants of 160 butterfly species were also recorded in the present study. Host plants of 18 species of butterflies were not available in the study area.

Temporal Shifts in Flower-Visiting Butterfly Communities and Their Floral Resources along a Vegetation Type Altered by Anthropogenic Factors

Habitat disturbance driven by human activities poses a major threat to biodiversity and can disrupt ecological interactions. Butterfly–plant mutualisms represent an ideal model system to study such anthropogenic impacts, as butterflies exhibit intimate dependencies on larval host plants and adult nectar sources, rendering them highly sensitive to habitat changes affecting the availability of these floral resources. This study examined flower-visiting butterfly communities and their associations with flowering plants in a landscape altered by anthropogenic factors in central Mexico. The study area encompassed a mosaic of vegetation types, including native juniper forests, agricultural lands, and introduced eucalyptus plantations, representing different degrees of human-induced habitat modification. Monthly surveys were conducted over a single year, covering both rainy and dry seasons, to analyze butterfly and plant diversity, community composition, and interactions. Results showed the highest diversity in juniper forests, followed by eucalyptus and agricultural sites. Seasonal turnover was the primary driver of community changes, with habitat-based segregation persisting within seasons. Butterfly diversity strongly correlated with flower abundance, while plant richness played a secondary role. SIMPER and indicator species analyses identified key taxa contributing to compositional dissimilarities among habitats and associated with specific vegetation types and seasons. Our research provides insights into temporal dynamics structuring butterfly–plant interactions across this forest disturbance spectrum, highlighting how habitat changes and seasonality shape these mutualistic communities in changing landscapes.

Conservation imperatives for swallowtail butterflies (Lepidoptera: Papilionidae): a case study in the north bank landscape of river Brahmaputra, Bodoland Territorial Region, India

The decline of swallowtail butterflies in forest habitats, which was not a significant concern about two decades ago, has now garnered attention worldwide, leading to their designation as globally endangered by the International Union for Conservation of Nature. A recent study conducted in the north bank landscape (NBL) of river Brahmaputra, specifically under the Bodoland Territorial Region in India, has shed light on the remarkable diversity of swallowtail butterfly species in the area. The study documented a total of 35 species representing eight different genera. Notably, three species are endemic to northeastern India, and 12 enjoy federal protection. The findings suggest that the studied landscape plays a crucial role in supporting larval host plants and adult resources of swallowtail butterflies along with the other abiotic factors. These butterflies depend on 25 plant species from six families as essential food sources. Unfortunately, these host plants, valued for their traditional medicinal properties, are being overexploited. Urgent conservation measures are imperative to safeguard the habitats of swallowtail butterflies and other wildlife in the NBL under Bodoland Territorial Region, as they face significant threats from practices such as agriculture, illegal tree felling, forest fires, and cattle farming.

Population status of the highly endangered Lycaena helle (Papilionoidea, Lycaenidae) in the Šumava Mts. two decades after establishment

The process of translocation of endangered species represents a useful conservation tool, but subsequent monitoring of translocated populations is often neglected, although it may supply critical information for conservation work. The EU-protected glacial relic Lycaena helle (Lycaenidae) went extinct in the Czech Republic, where a few lowland populations historically existed, in the 1950s. In 2002, a mountain-dwelling population was established in Šumava Mts., among grasslands and fenlands surrounding the abandoned village of Nové Údolí, from 2/33 ♂♂/♀♀ originating in the Türnitz Alps, Austria. The transferred population was surveyed a decade later and again 21 years after the transfer. During the second (2023) survey, we visited 212 grassland patches to record the species’ presence and relative abundance, and to ascertain its habitat preferences. A third of the surveyed patches were occupied by L. helle, in a total area of occupied grasslands was 66.6 ha scattered over 4.5 km2, with approximately 2,400 adult individuals, recorded up to 4.2 km from the original release point. L. helle typically occupies wet tussocky grasslands with closed edges, minimum management and high cover of its larval host plant, the mid-to late-successional forb Bistorta officinalis. It avoids drier managed grasslands rich in flowering plants. The expansion rate from the release point (0.1 km/year during the first decade, 0.2 km/year over two decades) was slower than in two earlier L. helle transfers, targeting Morvan and Forez Mts., France (≈ 0.5 km/year in both cases). We attribute this to the relief of the release locality, a­ shallow depression surrounded by higher-elevated forested ridges, but we expect faster expansion once the continuous woodlands are crossed.

Impacts of assisted migration: An introduced herbivore has short‐term and long‐term effects on its native host plant population

AbstractAssisted migration consists of the introduction of a species to previously inhabited areas or to new suitable regions. Such introductions have been touted as a viable tool for conserving the earth's biodiversity. However, both the likely success of assisted migrations and the impacts on local communities are hotly debated. Empirical data on the local impacts of assisted migration are particularly lacking. We examined the short and long time‐scale effects of herbivory on Lonicera involucrata (Richards) Banks ex. Spreng (Caprifoliaceae) after an introduction of Euphydryas gillettii Barnes (Lepidoptera: Nymphalidae, Melitaeini) to Gunnison County, Colorado, USA, via an assisted migration in 1977. The plant is the primary larval host plant for the butterfly. We quantified plant seed production, plant survival, and population stage structure in two sets of observational experiments. We found that herbivory by E. gillettii increased L. involucrata reproduction on an annual time scale, independent of plant size and local microhabitat characteristics. Over the time since the butterfly's introduction, herbivory by E. gillettii resulted in a plant population structure biased toward smaller plants in the butterfly introduction and satellite sites compared with sites without the butterfly. Our results highlight the importance of studying the effects of assisted migrations on native populations at different temporal scales. As assisted migration becomes an indispensable tool for species conservation, our work adds to the understanding of the multi‐trophic impacts of assisted introductions on local populations and communities.

Dose-dependent dynamics of densovirus infection in two nymphalid butterfly species utilizing native or exotic host plants

Insects are attacked by a diverse range of microbial pathogens in the wild. In herbivorous species, larval host plants frequently play a critical role in mediating susceptibility to infection. Characterizing such plant-mediated effects on herbivore-pathogen interactions can provide insight into patterns of infection across wild populations. In this study, we investigated the effects of host plant use by two North American butterflies, Euphydryas phaeton (Nymphalidae) and Anartia jatrophae (Nymphalidae), on entomopathogen infection across a range of three doses. Both of these herbivores recently incorporated the same exotic plant, Plantago lanceolata (Plantaginaceae), into their host range and are naturally infected by the same entomopathogen, Junonia coenia densovirus (Parvoviridae), in wild populations. We performed two factorial experiments in which E. phaeton and A. jatrophae were reared on either P. lanceolata or a native host plant [Chelone glabra (Plantaginaceae) for E. phaeton; Bacopa monnieri (Plantaginaceae) for A. jatrophae] and inoculated with either a low, medium, or high dose of the virus. In E. phaeton, the outcomes of infection were highly dose-dependent, with inoculation with higher viral doses resulting in faster time to death and greater mortality. However, neither survival nor postmortem viral burdens varied depending upon the host plant that was consumed. In contrast, host plant use had a strong effect on viral burdens in A. jatrophae, with consumption of the exotic plant appearing to enhance host resistance to infection. Together, these results illustrate the variable influences of host plant use on herbivore resistance to infection, highlighting the importance of investigating plant-herbivore relationships within a tritrophic framework.

Conserving apollo butterflies: habitat characteristics and conservation implications in Southwest Finland

The conservation of insects, particularly endangered species such as the Apollo butterfly, is a pressing global concern. Understanding the habitat requirements and factors influencing species occupancy is crucial for designing effective conservation strategies. We focused on investigating the habitat characteristics expected to affect the occupancy of the nationally endangered Apollo butterfly in Southwest Finland. We conducted field surveys and GIS analysis to assess the impact of larval host plant and adult nectar resources, habitat encroachment, elevation, connectivity, and spatial variation on Apollo larval occupancy in rocky outcrop habitats. We found that rocky outcrops with abundant host plants and those less isolated from nectar patches play a significant role in supporting Apollo reproduction, whereas encroachment, specifically increased tree volume, negatively affected occupancy. We additionally observed spatial variation in occupancy across different blocks within the study area. Our findings emphasise the importance of resource availability for Apollo butterflies and highlight the dynamic nature of their habitat requirements. Maintaining a network of intact rocky outcrops with suitable resources is essential for the long-term persistence of the Apollo butterfly population in the region.Implications for insect conservation: Our research underscores the critical need to protect and restore habitats for the Apollo butterfly, particularly by addressing threats such as habitat encroachment and construction projects that pose risks to their breeding sites.

Travniški postavnež <i>Euphydryas aurinia</i> (Rottemburg, 1775) (Lepidoptera: Nymphalidae) na Ljubljanskem barju: prvi vpogled v gostiteljske rastline in pregled razširjenosti

The article presents the first insight into the host plants of the marsh fritillary (Euphydryas aurinia) in the Ljubljansko barje region and provides recent information on the species distribution in this area. There are two permanent populations of the marsh fritillary in the region, i.e. in the Draga Valley near Ig and the valley of Strajanov breg stream. The main larval host plant at Ljubljansko barje is devil's-bit scabious (Succisa pratensis). Preadult stages have also been found on willow gentian (Gentiana asclepiadea) and southern succisella (Succisella inflexa). The expanding diversity of host plants increases the likelihood of long-term survival of the species. Therefore, understanding the local ecology of the preadult stages is crucial for planning effective management strategies for the species.

Adult Feeding Experience Determines the Fecundity and Preference of the Henosepilachna vigintioctopunctata (F.) (Coleoptera: Coccinellidae).

Both larvae and adults of the Henosepilachna vigintioctopunctata feed on leaves of potatoes, tomatoes, and eggplants. Given the variation in planting times of host plants in the Jianghan Plain, host switching between larvae and adults of H. vigintioctopunctata is inevitable to ensure continuous food availability. We evaluated the effect of consistent versus diverse larval and adult host plant feeding experience on growth performance, fecundity, longevity, and feeding preferences of H. vigintioctopunctata through match-mismatch experiments. Host plant quality significantly influences larval development and adult reproduction. Potatoes are identified as the optimal host plant for H. vigintioctopunctata, whereas eggplants significantly negatively affect the adult fecundity. Adult stage host feeding experience determines the fecundity of H. vigintioctopunctata, irrespective of the larval feeding experience. The fecundity of H. vigintioctopunctata adults on eggplant leaves remains significantly lower than that observed on potato leaves. Similarly, adult H. vigintioctopunctata demonstrate a preference for consuming potato leaves, irrespective of the larval feeding experience. Although host switching between larval and adult stages offers lesser benefits for the performance of herbivorous insects compared to a consistent diet with potato leaves, it maintains H. vigintioctopunctata population continuity amidst shortages of high-quality potato hosts.

Host genetics and larval host plant modulate microbiome structure and evolution underlying the intimate insect-microbe-plant interactions in Parnassius species on the Qinghai-Tibet Plateau.

Insects harbor a remarkable diversity of gut microbiomes critical for host survival, health, and fitness, but the mechanism of this structured symbiotic community remains poorly known, especially for the insect group consisting of many closely related species that inhabit the Qinghai-Tibet Plateau. Here, we firstly analyzed population-level 16S rRNA microbial dataset, comprising 11 Parnassius species covering 5 subgenera, from 14 populations mostly sampled in mountainous regions across northwestern-to-southeastern China, and meanwhile clarified the relative importance of multiple factors on gut microbial community structure and evolution. Our findings indicated that both host genetics and larval host plant modulated gut microbial diversity and community structure. Moreover, the effect analysis of host genetics and larval diet on gut microbiomes showed that host genetics played a critical role in governing the gut microbial beta diversity and the symbiotic community structure, while larval host plant remarkably influenced the functional evolution of gut microbiomes. These findings of the intimate insect-microbe-plant interactions jointly provide some new insights into the correlation among the host genetic background, larval host plant, the structure and evolution of gut microbiome, as well as the mechanisms of high-altitude adaptation in closely related species of this alpine butterfly group.

Host plant-mediation of viral transmission and its consequences for a native butterfly.

Pathogens play a key role in insect population dynamics, contributing to short-term fluctuations in abundance as well as long-term demographic trends. Two key factors that influence the effects of entomopathogens on herbivorous insect populations are modes of pathogen transmission and larval host plants. In this study, we examined tritrophic interactions between a sequestering specialist lepidopteran, Euphydryas phaeton, and a viral pathogen, Junonia coenia densovirus, on its native host plant, Chelone glabra, and a novel host plant, Plantago lanceolata, to explore whether host plant mediates viral transmission, survival, and viral loads. A two-factor factorial experiment was conducted in the laboratory with natal larval clusters randomly assigned to either the native or novel host plant and crossed with either uninoculated controls or viral inoculation (20% of individuals in the cluster inoculated). Diapausing clusters were overwintered in the laboratory and checked weekly for mortality. At the end of diapause, all surviving individuals were reared to adulthood to estimate survivorship. All individuals were screened to quantify viral loads, and estimate horizontal transmission postmortem. To test for vertical transmission, adults were mated, and the progeny were screened for viral presence. Within virus-treated groups, we found evidence for both horizontal and vertical transmission. Larval clusters reared on the native host plant had slightly higher horizontal transmission. Survival probability was lower in clusters feeding on the native host plant, with inoculated groups reared on the native host plant experiencing complete mortality. Viral loads did not differ by the host plant, although viral loads decreased with increased sequestration of secondary compounds on both host plants. Our results indicate that the use of a novel host plant may confer fitness benefits in terms of survival and reduced viral transmission when larvae feeding on it are infected with this pathogen, supporting hypotheses of potential evolutionary advantages of a host range expansion in the context of tritrophic interactions.

Wildlife-friendly garden practices increase butterfly abundance and species richness in urban and arable landscapes

Insect declines are being reported worldwide and private, residential gardens could provide refugia for these species under increasing land use change. Interest in wildlife-friendly gardening has increased, but many management recommendations lack a scientific evidence-base. We used a large citizen science scheme, the Garden Butterfly Survey (GBS), with data from over 600 gardens across Great Britain (2016–2021) to determine how the surrounding landscape influences the abundance and species richness of butterflies in gardens and whether wildlife-friendly gardening practices, such as having long grass and providing nectar plants, benefit butterflies. First, we show that GBS provides reliable estimates of species abundances by comparing with results from standardised, long-term monitoring data. Garden size and surrounding land use had significant effects on butterfly abundance and richness in gardens, including positive relationships with garden size, woodland and arable farmland and negative relationships with urbanisation. Both the presence and area of long grass in gardens were positively related to higher butterfly richness and abundance, with the latter being driven by butterflies that use grasses as larval host plants. These effects differed depending on the surrounding landscape, such that long grass resulted in higher garden butterfly abundance in landscapes dominated by arable farming, and higher abundance and richness in highly urbanised areas. The presence of flowering ivy (Hedera spp.) in gardens resulted in higher abundance of Celastrina argiolus holly blue which uses ivy as a larval host, and of Vanessa atalanta red admiral and Polygonia c-album comma, which favour it as a nectar source. Our work provides evidence that undertaking simple wildlife-friendly garden practices can be beneficial for attracting butterflies, particularly in heavily modified areas. With over 728,000 ha of gardens in Great Britain, the cumulative effect of leaving areas of lawn uncut and providing nectar and larval host plants could be key for helping biodiversity.