Larvae Of Known Species Research Articles

Description of laboratory-bred first zoea of Callinectes bellicosus Stimpson, 1859 (Crustacea, Portunidae)

ABSTRACT So far there are no descriptions of zoea larvae of known species of the genus Callinectes in the Pacific Ocean. Hence, the objective of this study is to describe the first larval stage of C. bellicosus and determine if the features of the zoea 1 stage are like the other species of Portunidae family. Twenty ovigerous females were captured at El Colorado lagoon in the Mexican Pacific, of the northern coast of Sinaloa, during September 2017. Females were transported to the aquaculture laboratory of the Instituto Politécnico Nacional (IPN); Interdisciplinary Research Centre for Integral Development (CIIDIR), Unit Sinaloa. The ovigerous females were isolated in tanks with aerated seawater at a salinity of 35 psu, and a temperature of 20.5 ± 1.5°C, until hatching. The zoea were fixed and preserved in 70% ethanol. The first zoea was dissected using glycerine for detailed examination under a stereoscope. In this study we observed that from C. bellicosus the lateral carapace spines were well-developed, the dorsolateral projections were found on the second and third abdominal segments, the abdominal segments 3–5 bore posterior lateral processes, the telson fork had three spines and there was an unarmed endopod middle segment at the first maxilliped, all these correspond to those morphological larval characters of the subfamily Portuninae.

Description of adults and larvae of Orientelmis parvula (Nomura amp; Baba, 1961) (Coleoptera: Elmidae) with their molecular phylogenetic analysis.

The elmid beetle Orientelmis parvula (Nomura Baba, 1961) is a rare and endangered species in Japan. Mouth parts and genitalia in adults of both sexes and larval morphology are described based on scanning electron microscope observations. The larva of the genus Orientelmis Shepard, 1998 is described for the first time. The systematic position of the genus is discussed based on the morphology and a phylogenetic tree inferred from the mitochondrial COI, and nuclear ArgK and 18S gene sequences. A new key for larvae of known species and genera of Japanese Elmidae is given.

A New Species of Meristogenys (Anura: Ranidae) from Sarawak, Borneo.

A cryptic Bornean torrent frog of the genus Meristogenys, which is divergent genetically and morphologically from all known congeners, is described from mountain streams of western Sarawak, East Malaysia (Borneo). The species occurs sympatrically with the type species of the genus, M. jerboa, but apparently differs from it in adult coloration and larval morphology, such as keratodont formulae and glands in tail fins. Females of the new species possess much larger and fewer eggs than in sympatric M. jerboa, suggesting significantly different reproductive traits between these species. A key to larvae of known species of the genus is provided.

Two new species of Parapentaneura (Diptera: Chironomidae: Tanypodinae) from Brazil, with keys to the males and immature stages

Two new species of Parapentaneura Stur, Fittkau et Serrano, 2006 (Diptera: Chironomidae: Tanypodinae) from southeastern Brazil are described and figured as male, pupa and larva: P. brunnescens and P. flavescens. Keys to the males, pupae and larvae of known species of Parapentaneura are provided.http://www.zoobank.org/urn:lsid:zoobank.org:pub:E6860EBE-B781-4DDA-9650-6E6EA3A0309E

Four new species of Clinotanypus Kieffer, 1913 (Diptera: Chironomidae: Tanypodinae) from Neotropical region

Four new species of Clinotanypus Kieffer, 1913 (Diptera: Chironomidae: Tanypodinae) from Brazil are described and figured as male and immatures: Clinotanypus caritus, Clinotanypus gymnos, Clinotanypus setosus and Clinotanypus striatus. Keys to the Neotropical males, pupae and larvae of known species of Clinotanypus are provided.http://www.zoobank.org/urn:lsid:zoobank.org:pub:DAE70C2F-15CA-4B72-9AE5-F601EAD76A7C

A new species of Lappodiamesa Serra-Tosio (Diptera: Chironomidae: Diamesinae) from the Russian Far East, with a key to known species of the genus.

The male, pupa, and larva of a new species, Lappodiamesa omelkoi, from the Russian Far East are described and figured. Keys to males, pupae, and fourth-instar larvae of known species of the genus are presented.

Two new species of Hudsonimyia Roback, 1979 (Diptera: Chironomidae: Tanypodinae) from Neotropical Region unveiled by morphology and DNA barcoding

Two new species of Hudsonimyia Roback, 1979 (Diptera: Chironomidae: Tanypodinae) are described and illustrated as male, pupa and larva. The generic diagnosis of pupa is emended and keys to males, pupae and larvae of known species are provided. The different life stages for one of the described species were associated by DNA barcodes.

The neotropical damselfly genusCora: new larval descriptions and a comparative analysis of larvae of known species (Odonata: Polythoridae)

The final larval stadium of four species of Cora are described and compared with known species in the genus. Cora skinneri Calvert, 1907, C. semiopaca Selys, 1878 and C. lugubris Navas, 1934 are described and illustrated for the first time using material from Costa Rica for the first two and from Colombia for the latter. A redescription of C. marina Selys, 1868 from specimens collected in Costa Rica is also included for comparison. Although all species are very similar as larvae, two major groups can be differentiated based on the shape of the caudal gills. The three species here described for the first time are very similar, but can be separated from each other using a combination of characters. Se describen los estadios finales de las larvas de tres especies de Cora y se comparan con las especies conocidas en el genero. Cora skinneri Calvert, 1907, C. semiopaca Selys, 1878 y C. lugubris Navas, 1934 son descritas e ilustradas por primera vez usando material de Costa Rica para las dos primeras y de Colomb...

Two new species of Larsia Fittkau, 1962 (Diptera: Chironomidae: Tanypodinae) from Neotropical Region, with a checklist of Larsia species of the world

Two new species of Larsia Fittkau, 1962 are described and figured as male, pupa and larva. Diagnosis to male of this genus is emended. An annotated checklist of the world’s valid Larsia species is given and keys to pupae and larvae of known species in the Neotropical region are provided.

<p class="Body" align="left">A new species of Arctodiamesa Makarchenko (Diptera: Chironomidae: Diamesinae) from the Russian Far East, with a key to known species of the genus

The male, pupa, and larva of a new species, Arctodiamesa marinae, from the Russian Far East are described and figured. Keys to males, pupae, and fourth-instar larvae of known species of the genus are presented.

Characterization of Insect Cell Lines: Heteroduplex Analysis Employing a Mitochondrial 16S Ribosomal RNA Gene Fragment

Routine cell line characterization procedures are not adequate for characterizing the cell lines of insect origin. Ribosomal RNA (rRNA) gene sequences and their comparisons have been used successfully for delineating species and phylogenetic analysis. Using similar principles, we have standardized a protocol for the confirmation of species identity of insect cell lines. The procedure includes PCR amplification of the mitochondrial 16S rRNA gene fragment from the cell line and larvae of known insect species and heteroduplex analysis to detect the sequence variation in the PCR-amplified rRNA gene fragments. If the PCR fragment of the cell lines yields a homoduplex with the larvae of known species, then the cell line is conspecific with the larvae. If the larvae and cell line are of two different species, then the analysis exhibits multiple bands of heteroduplexes. The technique also allows detection of cross-contamination of culture having two insect cell lines belonging to two different species.

A METHOD FOR DEVELOPING BRACHYURAN EGGS IN VITRO1

The larvae of six species of Brachyura have been successfully hatched by detaching the eggs from the female, placing them in compartmented boxes, and maintaining them on an Eberbach variable speed shaker at constant temperatures at different salinities. In addition to providing thousands of normal larvae of known species for experimental purposes, the method has the following advantages: it provides adequate aeration, prevents the spread of fungi and protozoans throughout the egg mass, and permits the use of a relatively small number of eggs from the same animal in a limited space.