Largest Inhibition Zone Diameter Research Articles

Synthesis of Zinc Nanoparticles in Hibiscus sabdariffa Plant Extract and Investigation of Its Antimicrobial Properties

Background: The use of nanotechnology in various biomedical and pharmaceutical fields is expanding rapidly. Objectives: The aim of this study was to synthesize zinc nanoparticles using Hibiscus sabdariffa plant extract and investigate their antimicrobial properties. Methods: In this experimental study, the aqueous extract of sour tea was mixed with a 0.1 M zinc sulfate solution at a 1:1 ratio and allowed to react for 15 minutes at room temperature to produce nanoparticles. The synthesis of zinc oxide nanoparticles was confirmed using spectrophotometric methods, measuring the average diameter of nanoparticles, X-ray diffraction, and scanning electron microscopy (SEM). The minimum inhibitory concentration (MIC) of the zinc oxide nanoparticles was then measured against standard strains. Results: The results showed that the largest inhibition zone diameter was observed at a concentration of 1024 μg/mL against Listeria monocytogenes, while the smallest inhibition zone was observed against Aeromonas hydrophila. At a concentration of 512 μg/mL, an inhibition zone of 10 mm was developed against two strains: Vibrio cholerae and Escherichia coli. Conclusions: Based on the results of this study, zinc oxide nanoparticles, along with the sour tea aqueous extract and zinc sulfate, exhibited antibacterial properties. However, the most pronounced antimicrobial effects were observed with the zinc oxide nanoparticles.

Molecular identification of endophytic bacterium DBA2 isolated from the leaf of binahong (Anredera cordifolia (Ten.) Steenis) and its antagonistic activity against bacteria associated with dental caries

Binahong plants (Anredera cordifolia (Ten.) Steenis) are known to contain secondary metabolites which have important biological properties. The current study focused on the endophytic bacteria which lives in the leaves of A. cordifolia (Ten.) Steenis. The aim of this study is to isolate and identify the endophytic bacteria which can produce antibacterial metabolites from the leaves of A. cordifolia (Ten.) Steenis. Two isolates, DBA1 and DBA2 were isolated and purified from the leaves of A. cordifolia. These isolates were subjected to the screening for their antagonistic activity against the bacteria associated with dental caries, which are Streptococcus mutans and Lactobacillus acidophilus using the disk-diffusion method. The strain of DBA2 exhibited the largest diameter of inhibition zone against both S. mutans (31,17 mm) and L. acidophilus (35,57 mm). While DBA1 exhibited the diameter of inhibition zone of 23,47 mm and 25,87 mm against S. mutans and L. acidophilus, as respectively. The strain of DBA2 was then subjected for molecular identification. The genomic DNA of DBA2 was extracted with the Geno Plus™ Genomic DNA Extraction Miniprep System and molecular identification was performed by PCR amplification and sequencing of the 16S rRNA gene. The amplicons were then purified and sequenced, before the 16S rRNA gene sequences were analysed by a Basic Local Alignment Search Tool (BLAST) search against the National Centre Biotechnology Information (NCBI) database. The endophytic bacterial strain DBA2 from the leaves of A. cordifolia was identified to be closely related to Bacillus sp., and the top match from the database search revealed a similarity value of 100% with the reference Bacillus sp. strain x20. Future studies are required to analyse the bioactive compounds of strain DBA2, which can be considered as a potential source for the new antibacterial drugs for the dental caries treatment.

Antibacterial Effectiveness Test of Methanol Extract of Red Galangal Rhizome (Alpinia purpurata (Vieill) K. Schum) Against Streptococcus pyogenes and Klebsiella pneumonia Bacteria

Red galangal rhizome (Alpinia purpurata (Vieill) K. Schum) is a medicinal plant belonging to the family Zingiberaceae. Red galangal rhizomes have antibacterial benefits because they contain active substances such as flavonoids, saponins, triterpenoids, alkaloids, and tannins. Streptococcus pyogenes causes bacterial infections of the skin and upper respiratory tract, while Klebsiella pneumonia is the bacteria that causes pneumonia. This study aims to determine the antibacterial effectiveness of methanol extract of red galangal rhizomes (Alpinia purpurata (Vieill) K. Schum) against Streptococcus pyogenes and Klebsiella pneumonia bacteria. This research is an experimental laboratory with a post-test-only controlled group design. Red galangal rhizomes were extracted using methanol solvent and tested for antibacterial effectiveness using the well method. There were 6 groups in the study, namely the chloramphenicol positive control group, the distilled water negative control group, and the 20%, 40%, 60%, and 80% concentration groups. The largest inhibition zone diameter was found at a concentration of 80%, with an average of 25.50 mm in Streptococcus pyogenes bacteria and 28.73 mm in Klebsiella pneumonia bacteria. In conclusion, methanol extract of red galangal rhizome (Alpinia purpurata (Vieill) K. Schum) effective against bacteria Streptococcus pyogenes and Klebsiella pneumonia.

Preparation of polyethersulfone ultrafiltration membrane coated natural additives toward antifouling and antimicrobial agents for surface water filtration

Chitosan (CS) and polyphenon 60 from green tea (PGT) as natural additives were used in the preparation of antifouling and antibacterial polyethersulfone (PES) ultrafiltration membranes via the surface coating method. The effect of additive coating on fouling resistance and antibacterial properties were evaluated. The membrane characterizations were carried out using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy, and surface hydrophilicity by contact angle. The results showed that the optimized modified membrane (1 wt% CS/3 wt% PGT-coated PES, denoted as CS1PGT3) demonstrated better hydrophilicity than the pristine PES membrane. In addition, the CS1PGT3 membrane exhibited high BSA rejection (99.99%), relatively high flux recovery (78.78%), and the highest water flux (46.83 L/m2.h) among all modified PES membranes. After the CS1PGT3 membrane was immersed in different soaking solutions (i.e., acid, alkaline, and neutral) for 21 days, the stability of CS and PGT groups on the PES membrane was also confirmed. Moreover, during examination using surface water filtration, the removal rates of chemical oxygen demand, turbidity, and aromatic compounds were increased from 64.30%, 34.23%, and 62.78% to 88.22%, 64.18%, and 84.21%, respectively. The largest inhibition zone diameter (7.83 mm of E. coli) and highest antibacterial efficiency (99% of E. coli) were achieved, suggesting the outstanding antibacterial properties of the optimized modified membrane. Thus, the incorporation of CS/PGT into PES membrane via the surface coating showed promising method to enhance antifouling and antibacterial properties.

Antibacterial Activity of Ethanol Extract, n-Hexane and Ethyl Acetate Fraction of Mundar (Garcinia forbesii) Pericarp

Garcinia forbesii is traditionally used as medicine and cosmetics. The aimed was to determine antibacterial activity of ethanolic extract and fraction of Garcinia forbesii pericarp against Staphylococcus aureus and Propionibacterium acne based on the diameter of inhibition zone, the minimum inhibitory concentration (MIC) and the minimum killing concentration (MBC). The results showed that the largest inhibition zone diameter against Staphylococcus aureus and Propionibacterium acne was indicated by ethyl acetate fraction of 5.08±1.020 mm and 14.33±3.326 mm. The MIC value of ethanol extract against Staphylococcus aureus and Propionibacterium acne bacteria was >1.5%, n-hexane and ethyl acetate fraction had the same results, namely against Staphylococcus aureus >1.5% and Propionibacterium acne at 1.5%. The MBC value was only obtained for fraction samples of Propionibacterium acne at concentration of 1.5%. It was concluded that ethyl acetate had strong antibacterial activity against Propionibacterium acne based on the value of inhibition zone diameter, MIC, and MBC.

Antibacterial Test of Teki Grass Extract (Cyperus Rotundus) in Inhibiting Escherichia Coli and Salmonella Typhi

This study aims to determine the antibacterial ability of Cyperus rotundus extract in inhibiting the growth of Escherichia coli and Salmonella typhi by measuring the diameter of the inhibition zone. This research method used a completely randomized design (CRD) and LSD test as a follow-up test with one factor, namely variations in the concentration of C. rotundus extract 20%, 40%, 60%, 80% and 100%, distilled water as a negative control and the antibiotic chloramphenicol as a positive control. The results showed that the extract of C. rotundus could inhibit the growth of Escherichia coli with the largest inhibition zone diameter indicated by a concentration of 100% which was 2.33mm and the smallest inhibition zone diameter was found at a concentration of 20%, namely 1.00 mm. While in Salmonella typhi the largest inhibition zone was shown at a concentration of 100%, namely 3, 03mm and the smallest inhibition zone was indicated by a concentration of 20%, namely 1.07 mm. The results of the ANOVA and LSD tests with 95% confidence test showed that there was a difference in the average diameter of the inhibition zone in each treatment with variations in the concentration of C. rotundus extract with a concentration of 80% as the most effective.

The Investigation of the Antibacterial Activity of Eryngium caucasicum trautv, Saponaria officinalis, Froriepia subpinnata, Malva sylvestris, and Urtica dioica

Background: Regarding the increasing resistance of bacteria against antibiotics and the existence of antibacterial compounds in plants, we here investigated the antimicrobial effects of the methanolic extracts of several plants on pathogenic bacteria. Methods: Ten grams of the dry ingredients of Eryngium caucasicum trautv (leaf), Saponaria officinalis (flower), Froriepia subpinnata (leaf), Malva sylvestris (leaf), and Urtica dioica (leaf) was poured in 100 mL of methanol and shaken for 24 hours. After 24 hours, the extracts were strained and dried in the oven, and their antimicrobial activity at the 100 mg/mL concentration was evaluated against the mentioned bacteria using the sinkhole method. Results: The plant extracts inhibited the growth of all bacteria in various degrees. Among all the plant extracts used, that of F. subpinnata delivered the largest diameter of growth inhibition zone (6 mm) against S. dysenteriae, and the extracts of E. caucasicum and F. subpinnata showed the largest diameter of inhibition zone against Xanthomonas translucens. Also, the extract of M. sylvestris delivered the largest inhibition zone diameter (8 mm) against E. coli. Conclusions: Our results showed that the methanolic extract of E. caucasicum was the most effective plant extract against S. dysenteriae, Rathayibacter tritici, and L. monocytogenes, but M. sylvestris extract revealed the greatest impact on E. coli. It is recommended to further investigate the antibacterial compounds of these plants as potential treatments for bacterial infections.

SOCIALIZATION OF ANTIBACTERIAL ACTIVITY OF KERSEN LEAF EXTRACT (Muntingia calabura L) AGAINST BACTERIA Staphylococcus aureus

Kersen (Muntingia calabura) is a shady plant that is usually used as a shade. Cherry leaves contain several secondary metabolites such as flavonoid, tannin and terpenoid. Flavonoid can inhibit bacterial growth by denaturing proteins and damaging cell membranes and dissolving fats contained in cell walls. According to the World Health Organization as many as 25 million deaths worldwide in 2011, one third were caused by infectious diseases. One of the most common infectious diseases caused by the bacterium Staphyloccus aureus. This socialization aims to provide information to the public about the antibacterial ability of kersen leaves against staphylococus aureus. The concentration of the extract used was 15%, 30%, 45% and 60% and erythromycin as a positive control. From the test results, it can be concluded that the largest inhibition zone diameter is at a concentration of 60% with an inhibition zone diameter of 6.78 mm and the smallest at a concentration of 30%, which is 0.42 mm. Concentration of 45% has (weak) activity inhibiting bacterial growth with an inhibition zone of 2.13 mm, while at a concentration of 15% it has no activity in the inhibition zone of bacterial growth

Antibacterial Activity Test of Meniran Herb Extract (Phyllanthus Niruri L.) against Staphylococcus Epidermidis and Klebsiella Pneumoniae

An antibacterial test of meniran herb extract (Phyllanthus niruri L.) against Staphylococcus epidermidis and Klebsiella pneumoniae has been carried out. Meniran herb powder was screened for phytochemicals to assess the secondary metabolites contained in it, then meniran herbs were extracted using the soxhletation method in stages with various solvents (n-hexane, ethyl acetate, and 70% ethanol). The disc diffusion method was used to test the inhibition zone diameter of n-hexane extract, ethyl acetate extract, and ethanol extract against Staphylococcus epidermidis and Klebsiella pneumoniae. Meniran herb extract was diluted with a concentration series using dimethylsulfoxide. Meniran herb extracts from the three types showed the best inhibition zone diameter when tested for minimum inhibitory concentration (MIC) by the solid dilution method. The results showed that meniran herb powder contained flavonoids, saponins, tannins, glycosides, and steroids/terpenoids. Antibacterial tests showed that ethyl acetate and 70% ethanol extracts had antibacterial activity only against Staphylococcus epidermidis. The antibacterial activity of the two meniran herb extracts had the largest inhibition zone diameter against Staphylococcus epidermidis at a concentration of 50%, which was 12,33 mm for the ethyl acetate extract and 16,00 mm for the ethanol extract. The MIC test of the ethanol extract of the meniran herb, which had the best antibacterial activity, found that at a concentration of 2,5% it was able to inhibit the growth of Staphylococcus epidermidis.

Titanium platelet-rich fibrin (T-PRF) as high-capacity doxycycline delivery system.

Titanium platelet-rich fibrin (T-PRF), a second-generation autogenous blood concentrate with tough and thick fibrin meshwork activated by a titanium tube, was used as a drug carrier for doxycycline (Doxy) by injection. The objective of this study is to evaluate the loading capacity of T-PRF, release kinetics of doxycycline-loaded T-PRF, and its antibacterial effects against S. aureus and P. aeruginosa. The T-PRF and collagen were loaded with Doxy as T-PRF/Doxy and Collagen/Doxy, and their release and antibacterial activities against S. aureus and P. aeruginosa were investigated. Chemical characterization and morphological analysis were performed. In comparison with collagen, approximately sevenfold more Doxy, 281mg/g, was loaded into T-PRF. It was found that 25% of the loaded Doxy was released from T-PRF compared to only 12% from collagen within 72h. The largest inhibition zone diameter (IZD) was observed for T-PRF/Dox with 32 ± 6mm and 37 ± 5mm for P. aereginosa and S. aureus, respectively. However, only 10 ± 5mm and 10 ± 6mm IZD were observed for bare T-PRF, and no inhibition zone was observed for the Collagen/Doxy group. A dense fibrin structure was visualized on SEM images of the T-PRF/Doxy group compared to the T-PRF group. T-PRF has higher Doxy loading capacity and long-acting antibacterial effects compared to collagen. T-PRF was shown to have potential autogenous long-term drug-carrying capability for doxycycline. Also, the potential fibrinophilic properties of Doxy were observed to strengthen the structure of T-PRF. T-PRF is an autogenous drug career with high loading capacity and extended antibacterial effects for doxycycline. Doxycycline molecules can be visible on T-PRF fibers. This study suggests that T-PRF/Dox could be used as a proper antibiotic delivery device in the treatments of periodontitis and peri-implantitis.

ANTIBACTERIAL ACTIVITY OF KERSEN LEAF EXTRACT (MUNTINGIA CALABURA L) AGAINST STAPHYLOCOCCUS AUREUS

Kersen (Muntingia calabura) is a shady plant that is usually used as a shade. Cherry leaves contain several secondary metabolites such as flavonoid, tannin and terpenoid. Flavonoid can inhibit bacterial growth by denaturing proteins and damaging cell membranes and dissolving fats contained in cell walls. According to the World Health Organization as many as 25 million deaths worldwide in 2011, one third were caused by infectious diseases. One of the most common infectious diseases caused by the bacterium Staphyloccus aureus. The purpose of this study was to see whether the 96% ethanol extract of cherry leaves had antibacterial activity against Staphylococcus aureus. The concentration of the extract used was 15%, 30%, 45% and 60%. From the test results, it can be concluded that the largest inhibition zone diameter is at a concentration of 60% with an inhibition zone diameter of 6.78 mm and the smallest at a concentration of 30%, which is 0.42 mm. Concentration of 45% has (weak) activity inhibiting bacterial growth with an inhibition zone of 2.13 mm, while at a concentration of 15% it has no activity in the inhibition zone of bacterial growth.

Antibacterial efficacy of peracetic acid in comparison with sodium hypochlorite or chlorhexidine against Enterococcus faecalis and Parvimonas micra

BackgroundThe main goal of an endodontic treatment is a complete debridement of the root canal system; however, currently mechanical shaping and chemical cleaning procedures for this purpose have deemed non-satisfactory.MethodsThe efficacy of peracetic acid (PAA; 0.5, 1.0, 2.0%), as a root canal irrigation solution, against Enterococcus faecalis (DSM 20478) and Parvimonas micra (DSM 20468) when compared with the one of sodium hypochlorite (NaOCI; 1.0, 3.0, 5.0%), chlorhexidine digluconate (CHX; 0.12, 0.2, 2.0%) and 0.9% NaCI (as a control solution) was in vitro investigated with the agar diffusion and direct contact methods. The inhibition zone diameters observed with the agar diffusion test were determined. The viable bacterial counts (CFU/ml) were calculated with the direct method.ResultsThe agar diffusion test showed that all three root canal irrigation solutions had an efficacy against E. faecalis at all concentrations. The largest inhibition zone diameters against E. faecalis were observed with 5.0% NaOCI. At all three concentrations of PAA, NaOCI, and CHX, the inhibition zone diameter increased with increase in concentration. For P. micra, PAA had a similar inhibition zone diameter despite a concentration increase. In contrast, for NaOCI and CHX, the inhibition zone diameter increased with increasing concentration. 2.0% CHX produced the largest inhibition zone diameter against P. micra. For E. faecalis, only the comparison between 2.0% PAA and 5.0% NaOCI showed statistical significance (p = 0.004). For P. micra the efficacy comparison between the lowest, middle, and highest concentrations of each solution, a statistical significance (p < 0.05) was found for all three solutions. After direct contact with PAA, NaOCI and CHX, no viable bacteria could be determined for either P. micra or E. faecalis.ConclusionsPAA had a similar antibacterial efficacy as the one of NaOCl and CHX when in direct contact with E. faecalis and P. micra. In the agar diffusion test, PAA showed a similar antibacterial efficacy as the one of CHX and a lower one as the one of NaOCl for E. faecalis.

Antibacterial Activity of Combination of Ethanol Extract of Pepermine Leaves (Mentha piperita L.) and Amikacin Against Klebsiella pneumonia, Staphylococcus aureus, and Escherichia coli

Peppermint leaves have been known to have antibacterial and antifungal activity. Amikacin is a semisynthetic derivative of kanamycin which is active against both Gram-positive and Gram-negative enteric bacteria. The combination of plant extracts with antibiotics is one way or alternative to overcome bacterial resistance to antibiotics. The purpose of this study was to determine the combined effect of the ethanolic extract of peppermint (Mentha piperita L.) and amikacin against Klebsiella pneumonia, Staphylococcus aureus, and Escherichia coli as well as the compounds contained in the ethanolic extract of peppermint (Mentha piperita L.) leaves. Antibacterial activity was tested using the disk diffusion method (Kirby Bauer) and the phytochemical screening test using the tube test method. The concentration of peppermint leaf ethanol extract for the combination test was 200 mg/mL and 400 mg/mL, for the concentration of amikacin used was 5 mg/mL with three comparisons made, namely 25:75, 50:50, and 75:25. The results showed a synergistic effect with the largest inhibition zone diameter at a ratio of 25:75 at a concentration of 200 mg/mL, which was 36.25±2.5 mm on Klebsiella pneumonia bacteria. While at a concentration of 400 mg/mL the diameter of the largest inhibition zone was 40±1.63 mm in Klebsiella pneumoniae bacteria with a ratio of 75:25. The results of statistical tests using the t-test showed a significance value of 0.000 0.05 so that there was a significant difference in the administration of each concentration to the resulting inhibition zone. The results of the phytochemical screening test contained alkaloids, phenolic compounds, flavonoids, tannins.

Pengaruh lama maserasi daun ketapang merah (Terminalia Catappa L.) terhadap daya hambat Staphylococcus aureus dan Escherichia coli

Background: Bengal Almond leaves (Terminalia catappa L.) contain alkaloids, saponins, tannins, polyphenols, quinones, flavonoids and triterpenoids that can function as antibacterial.Objectives: This research was conducted to determine the effect of maceration time of red ketapang leaves (Terminalia catappa L.) on the growth of Staphylococcus aureus and Escherichia coli. Methods: This study was experimental using a completely randomized design (CRD) consisting of 6 treatments, that P0 (aquades), P1 (red ketapang leaf extract with 1 day maceration time), P2 (2 days maceration) P3 (3 days maceration) , P4 (4 days maceration) and P5 (5 days maceration) with 4 replications.Results: Anova test showed that macerated red ketapang leaf extract significantly affected the growth of Staphylococcus aureus and Escherichia coli (p= 0.000). Duncan's further test showed that the largest inhibition zone diameter in Staphylococcus aureus was formed by P2 (2 days maceration time) which was 18.62 mm and there was no significant difference between treatments. Meanwhile, the largest inhibition zone diameter against Escherichia coli was formed by P1 (1 day maceration time) which was 20.25 mm and there was a significant difference between treatments. Conclusion: Ketapang red leaf extract with different maceration times can inhibit the growth of Staphylococcus aureus and Escherichia coli.

Enhanced hydrophilicity and antifouling performance of PES-C/emodin ultrafiltration membrane

In this article, an ultrafiltration membrane was fabricated from phenolphthalein polyethersulfone (PES-C) modified with emodin using a phase-inversion method. ATR-FTIR and UV-vis analysis showed that emodin had good compatibility with the PES-C ultrafiltration membrane. SEM showed that the prepared ultrafiltration membranes consisted of a porous skin layer and a macroporous support sublayer. The contact angle value of the pure PES-C ultrafiltration membrane was 77.71° and that of the PES-C ultrafiltration membrane blended with 0.105 wt.% emodin decreased to 65.71°, which explained the fact why its pure water flux significantly increased from 190 L/m2·h to 387 L/m2·h. The antifouling properties of the obtained ultrafiltration membranes were assessed by static protein adsorption, bacterial adhesion, antibacterial tests, and filtration experiments with BSA. The PES-C (13.895 wt.%)/emodin (0.105 wt.%) ultrafiltration membrane presented the lowest protein adsorption rate (1.44%), the highest flux recovery ratio (57%), and the largest inhibition zone diameter (3.0 ± 0.06 mm). Compared with that of the pure PES-C ultrafiltration membrane, the bacterial adhesion effect of the PES-C/emodin (0.105 wt.%) ultrafiltration membrane was significantly reduced. In addition, PES-C incorporated into the emodin ultrafiltration membrane had excellent stability in a deionized water system.

Relationships of irrigation water and soil physical and chemical characteristics with yield, chemical composition and antimicrobial activity of Damask rose essential oil.

Damask rose (Rosa damascena Mill.) is an aromatic medicinal plant rich in bioactive compounds with high value in the food, pharmaceutical and cosmetic industries. Knowledge of the factors affecting the quantitative and qualitative properties of the compounds in its essential oil (EO) and the bioactivity of this EO is important in optimizing Damask rose cultivation and production. This research studied, for the first time, the effects of irrigation water and soil chemical and physical characteristics on the EO yield of this important commercial species and on it chemical composition and antimicrobial activity. The results showed the significant effect of crop cultivation site on yield, chemical composition and inhibition zone diameter (IZD) at the 1% significance level. The highest EO yield (~0.0266%), which belonged to the Noushabad site (EON), resulted from the increased soil electrical conductivity (EC) and the higher sand, gypsum and lime contents and irrigation water salinity. Analysis of the chemical composition of the EOs showed that their main compounds at all three crop sites were citronellol, nonadecane, heneicosane and geraniol. The EO obtained from the Yazdel site (EOY) had the highest contents of citronellol and geraniol (~29.05% and ~6.85%) that were directly correlated with soil potassium and phosphorus contents and inversely correlated with soil acidity and EC and its lime, nitrogen, and organic carbon contents. Antimicrobial assays indicated that the EO extracted from the Sefidshahr site (EOS), which had the largest inhibition zone diameter (~14.67 mm) for Aspergillus brasiliensis (IZD~14.67 mm) and the lowest MIC (~31.25 μg/mL) for Staphylococcus aureus and Pseudomonas aeruginosa, exhibited efficacy similar to that of rifampin, probably due to the dominance of the alkanes in it. The EOY and EOS also exhibited the strongest inhibitory and lethal activity against Candida albicans (MIC and MBC <15.63 μg/mL for EOY and MIC and MBC = 62.5 μg/mL for EOS), which were six and four times stronger than those of nystatin, respectively. Therefore, the selected EOs can act as a potentially promising strategy for fighting microbial strains.

Antimicrobial effects of Glycyrrhiza glabra extract, iron oxide nanoparticles, and Lactobacillus rhamnosus on a biofilm composed of Pseudomonas aeruginosa in glass, wood, and polysteell

Biofilm formation is a pathogenicity factor of Pseudomonas aeruginosa, which causes inherent resistance to a wide range of antibiotics in the strains. The present study aimed to compare the inhibitory effects of Glycyrrhiza glabra (licorice) extract, iron oxide nanoparticles, and Lactobacillus rhamnosus suspension on a biofilm composed of P. aeruginosa in various levels of glass, wood, and polysteel. This descriptive, cross-sectional study assessed the effects of Glycyrrhiza glabra extract, iron oxide nanoparticles, and Lactobacillus rhamnosus suspension on the standard biofilm of P. aeruginosa 1601PTCC on glass, steel, and wood surfaces. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also calculated. The obtained results showed that each antimicrobial agent had different effects on P. aeruginosa, and the MIC and MBC exerted inhibitory properties. In addition, the largest inhibition zone diameter was 28 mL due to the effect of the Glycyrrhiza glabra extract on free bacteria in the volume of 180 microliters, and the highest inhibitory level was observed on the polysteel and glass surfaces with the inhibition zone diameter of 20-20.66 millimeters in the volume of 180 microliters. The highest inhibition in the bacterial biofilm was observed on the polysteel surface, and a significant difference was also denoted in this regard with the glass and wood surfaces (p <0.05). Therefore, it could be concluded that licorice (Glycyrrhiza glabra L.) had more significant antimicrobial properties compared to the iron oxide nanoparticles and Lactobacillus rhamnosus suspension.

Physical, morphological and antibacterial properties of lime essential oil nanoemulsions prepared via spontaneous emulsification method

Lime essential oil (EO) nanoemulsions from key lime (Citrus aurantifolia), kaffir lime (Citrus hystrix) and calamansi lime (Citrofortunella microcarpa) were produced by using spontaneous emulsification method and their particle size, polydispersity index (PDI), turbidity, morphology and antibacterial properties were investigated. Lime EO nanoemulsions were produced by mixing 5% (v/v) of oil phase (lime EO and corn oil at the ratio of 8:2) with 15% (v/v) of Tween 80 and 80% (v/v) of deionized water using magnetic stirring at 750 rpm and 25 °C. Key lime EO nanoemulsion showed the smallest particle size (21 nm), lowest PDI value (0.444) and lowest turbidity (99.0% of transmittance) followed by kaffir and calamansi lime. Transmission electron microscopy (TEM) micrographs revealed sphere-shaped oil particles with different particle size. Freshly prepared calamansi lime EO nanoemulsion was the most effective against Escherichia coli, Salmonella spp, and Staphylococcus aureus by exhibiting the largest diameter of inhibition zone (8.34, 7.71, and 9.98 mm, respectively). Key lime EO nanoemulsion showed the lowest reduction in the antibacterial activity after 1 month of storage at room temperature. The lime EO nanoemulsions showed great potential to be incorporated into water-based food products and beverages as flavouring and antimicrobial agents.

ANTIBACTERIAL ACTIVITY OF COMPOUNDS FROM Azolla pinnata EXTRACTED USING SOXHLET AND SUPERCRITICAL FLUID (SFE) METHODS

The propagation, extraction, and antibacterial studies of Azolla pinnata were carried out in this study. The propagation involved two fertilizers, which were chicken manure and inorganic AB fertilizer. The dry yield was extracted using two methods, which were Soxhlet and supercritical fluid extraction (SFE). Methanolic extracts were obtained and subjected to several antibacterial tests, which include the disk diffusion assay, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests, against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The results showed that AB fertilizer produced more dry yield compared to chicken manure. The extract yield from Soxhlet exhibited a higher yield than that of SFE, recording its highest at 21.20%. The findings of antibacterial tests revealed that all extracts inhibited the growth of B. subtilis and S. aureus, but none showed inhibition against E. coli and P. aeruginosa. The largest diameter of inhibition zone of 2.67 ± 1.53 mm was obtained by E2 (CM-SFE), with a MIC value of 0.125 mg/mL against B. subtilis. High MBC values further confirm that the mechanism of inhibition against B. subtilis and S. aureus were due to the bacteriostatic properties of the extracts tested.

Aktivitas Antibakteri Ekstrak Dan Fraksi Herba Kemangi (Ocimum Sanctum L) Terhadap Staphylococcus Aureus Dan Pseudomonas Aeruginosa

Penyakit infeksi masih menjadi salah satu masalah kesehatan masyarakat khususnya di negara berkembang. Etiologi secara umum disebabkan oleh Staphylococcus aureus dan Pseudomonas aeruginosa. Herba kemangi (Ocimum sanctum L) merupakan tanaman yang dikenal sebagai lalapan ini memiliki khasiat sebagai antioksidan, antikanker dan antimikroba namun belum banyak informasi ilmiah sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui kandungan kimia dan aktivitas antibakteri dari ekstrak etanol, fraksi n-heksan, etil asetat dan air Herba kemangi terhadap pertumbuhan Staphylococcus aureus dan Pseudomonas aeruginosa secara difusi dan dilusi.&#x0D; Herba kemangi maserasi menggunakan etanol 96%. Ekstrak yang diperoleh kemudian disuspensi dengan air selanjutnya difraksinasi dengan n-heksan dan etil asetat. Ekstrak Herba kemangi beserta fraksi yang didapatkan selanjutnya diuji aktivitas antibakterinya dengan konsentrasi 12,5%, 25% dan 50% menggunakan metode difusi. KHM dan KBM dari fraksi teraktif bisa diketahui menggunakan uji dilusi dengan konsentrasi 0,78, 1,56, 3,125, 6,25%, 12,5%, 25% dan 50%. Kontrol positif ciprofloksasin 5μg/15μl dan kontrol negatif menggunakan tween 80 5% steril. Identifikasi golongan senyawa dilakukan dengan metode uji tabung.&#x0D; Hasil uji aktifitas antibakteri menunjukan diameter zona hambatan pada bakteri gram negatif secara umum cendrung lebih besar dibandingkan bakteri gram positif. Diameter zona hambat terbesar pada bakteri Pseudomonas aeruginosa pada fraksi etil asetat Herba kemangi konsentrasi 50% dengan diameter zona hambat (10 mm), pada bakteri Staphylococcus aureus terdapat pada fraksi etil asetat pada konsentrasi 50 % memiliki daya hambat sebesar (8,6 mm). Hasil identifikasi kandungan kimia menunjukkan bahwa dalam ekstrak dan fraksi herba kemangi mengandung senyawa flavonoid, saponin dan steroid dan tanin&#x0D; &#x0D; Kata kunci: Antibakteri, Herba kemangi (Ocimum sanctum L), Staphylococcus aureus, Pseudomonas aeruginosa