Largest Carbon Pool Research Articles

Multi-Scenario Simulation of Land Use and Assessment of Carbon Stocks in Terrestrial Ecosystems Based on SD-PLUS-InVEST Coupled Modeling in Nanjing City

In the context of achieving the goal of carbon neutrality, exploring the changes in land demand and ecological carbon stocks under future scenarios at the urban level is important for optimizing regional ecosystem services and developing a land-use structure consistent with sustainable development strategies. We propose a framework of a coupled system dynamics (SD) model, patch generation land-use simulation (PLUS) model, and integrated valuation of ecosystem services and trade-offs (InVEST) model to dynamically simulate the spatial and temporal changes of land use and land-cover change (LUCC) and ecosystem carbon stocks under the NDS (natural development scenario), EPS (ecological protection scenario), RES (rapid expansion scenario), and HDS (high-quality development scenario) in Nanjing from 2020 to 2040. From 2005 to 2020, the expansion rate of construction land in Nanjing reached 50.76%, a large amount of ecological land shifted to construction land, and the ecological carbon stock declined dramatically. Compared with 2020, the ecosystem carbon stocks of the EPS and HDS increased by 2.4 × 106 t and 1.5 × 106 t, respectively, with a sizable ecological effect. It has been calculated that forest and cultivated land are the two largest carbon pools in Nanjing, and the conservation of both is decisive for the future carbon stock. It is necessary to focus on enhancing the carbon stock of forest ecosystems while designating differentiated carbon sink enhancement plans based on the characteristics of other land types. Fully realizing the carbon sink potential of each ecological functional area will help Nanjing achieve its carbon neutrality goal. The results of the study not only reveal the challenges of ecological conservation in Nanjing but also provide useful guidance for enhancing the carbon stock of urban terrestrial ecosystems and formulating land-use planning in line with sustainable development strategies.

Short Communication: Carbon stock of mangrove forest in Pantai Sederhana, Bekasi District, West Java, Indonesia

Abstract. Erniasari I, Hernawan E, Mulyaningrum. 2024. Short Communication: Carbon stock of mangrove forest in Pantai Sederhana, Bekasi District, West Java, Indonesia. Biodiversitas 25: 2974-2980. Mangrove forests play an important role in the carbon cycle because this forest can store greater amounts of carbon than other ecosystems including tropical rainforests. Mangrove forests in Bekasi District, West Java, Indonesia have enormous potential to absorb carbon in this industrial area. In this research, we estimate the aboveground carbon (AGC), belowground carbon (BGC), and soil organic carbon (SOC) of mangrove forest in Pantai Sederhana, Muaragembong Subdistrict, Bekasi District. Carbon estimation of AGC and BGC was carried out using the allometric method, while SOC at four depth intervals (0-15, 15-30, 30-50, and 50-100 cm) was estimated using the LOI (Loss of Ignition) method. There were four mangrove species in Pantai Sederhana, namely Avicennia marina, Avicennia alba, Rhizophora apiculata, and Rhizophora mucronata. The total carbon stock was 329.54 Mg.C.ha-1, which was composed by aboveground carbon (114.51 Mg.C.ha-1), belowground carbon (61.46 Mg.C.ha-1) and soil carbon (153.56 Mg.C.ha-1). The largest carbon pool was contributed from soils with 47% of the total carbon stock. The result of this study can be used as the initial information of the importance of the mangrove ecosystem in Bekasi District.

Reducing the uncertainty in estimating soil microbial-derived carbon storage

Soil organic carbon (SOC) is the largest carbon pool in terrestrial ecosystems and plays a crucial role in mitigating climate change and enhancing soil productivity. Microbial-derived carbon (MDC) is the main component of the persistent SOC pool. However, current formulas used to estimate the proportional contribution of MDC are plagued by uncertainties due to limited sample sizes and the neglect of bacterial group composition effects. Here, we compiled the comprehensive global dataset and employed machine learning approaches to refine our quantitative understanding of MDC contributions to total carbon storage. Our efforts resulted in a reduction in the relative standard errors in prevailing estimations by an average of 71% and minimized the effect of global variations in bacterial group compositions on estimating MDC. Our estimation indicates that MDC contributes approximately 758 Pg, representing approximately 40% of the global soil carbon stock. Our study updated the formulas of MDC estimation with improving the accuracy and preserving simplicity and practicality. Given the unique biochemistry and functioning of the MDC pool, our study has direct implications for modeling efforts and predicting the land-atmosphere carbon balance under current and future climate scenarios.

Enhanced silicate weathering accelerates forest carbon sequestration by stimulating the soil mineral carbon pump.

Enhanced silicate rock weathering (ERW) is an emerging strategy for carbon dioxide removal (CDR) from the atmosphere to mitigate anthropogenic climate change. ERW aims at promoting soil inorganic carbon sequestration by accelerating geochemical weathering processes. Theoretically, ERW may also impact soil organic carbon (SOC), the largest carbon pool in terrestrial ecosystems, but experimental evidence for this is largely lacking. Here, we conducted a 2-year field experiment in tropical rubber plantations in the southeast of China to evaluate the effects of wollastonite powder additions (0, 0.25, and 0.5 kg m-2) on both soil organic and inorganic carbon at 0-10 cm depth. We found that ERW significantly increased the concentration of SOC and HCO3 -, but the increases in SOC were four and eight times higher than that of HCO3 - with low- and high-level wollastonite applications. ERW had positive effects on the accrual of organic carbon in mineral-associated organic matter (MAOM) and macroaggregate fractions, but not on particulate organic matter. Path analysis suggested that ERW increased MAOM mainly by increasing the release of Ca, Si, and Fe, and to a lesser extent by stimulating root growth and microbial-derived carbon inputs. Our study indicates that ERW with wollastonite can promote SOC sequestration in stable MOAM in surface soils through both the soil mineral carbon pump and microbial carbon pump. These effects may have been larger than the inorganic CDR during our experiment. We argue it is essential to account for the responses of SOC in the assessments of CDR by ERW.

Screening of environmental stimuli for the positive regulation of stomatal aperture in centipedegrass

Grasslands, the largest carbon pool in China, possess enormous potential for carbon sequestration. Increasing the stomatal aperture to increase the CO2 absorption capacity is a potential method to improve plant photosynthetic efficiency and ultimately enhance the carbon sequestration capacity of grass plants. Research on stomatal aperture regulation has focused mostly on Arabidopsis or crops, while research on grass plants in these areas is scarce, which seriously restricts the implementation of this grassland carbon sequestration strategy. Here, a widely used ecological grass, centipedegrass, was used as the experimental material. First, a convenient method for observing the stomatal aperture was developed. The leaves were floated in a potassium ion-containing open solution (67 mM KCl, pH 6.0) with the adaxial surface rather than the abaxial surface in contact with the solution and were cultivated under light for 1.5 h. Then, nail polish was applied on the adaxial surface, and a large number of open stomata were imprinted. Second, with the help of this improved method, the concentration‒response characteristics of the stomatal aperture to eleven environmental stimuli were tested. The stomatal aperture is dependent on these environmental stimuli in a concentration-dependent manner. The addition of 100 μM brassinolide led to the maximal stomatal aperture. This study provided a technical basis for manipulating stomatal opening to increase the carbon sequestration capacity of centipedegrass.

Long-term soil warming decreases soil microbial necromass carbon by adversely affecting its production and decomposition.

Microbial necromass carbon (MNC) accounts for a large fraction of soil organic carbon (SOC) in terrestrial ecosystems. Yet our understanding of the fate of this large carbon pool under long-term warming is uncertain. Here, we show that 14 years of soil warming (+4°C) in a temperate forest resulted in a reduction in MNC by 11% (0-10 cm) and 33% (10-20 cm). Warming caused a decrease in the content of MNC due to a decline in microbial biomass carbon and reduced microbial carbon use efficiency. This reduction was primarily caused by warming-induced limitations in available soil phosphorus, which, in turn, constrained the production of microbial biomass. Conversely, warming increased the activity of soil extracellular enzymes, specifically N-acetylglucosaminidase and leucine aminopeptidase, which accelerated the decomposition of MNC. These findings collectively demonstrate that decoupling of MNC formation and decomposition underlie the observed MNC loss under climate warming, which could affect SOC content in temperate forest ecosystems more widespread.

Mechanisms Underpinning the Net Removal Rates of Dissolved Organic Carbon in the Global Ocean

AbstractWith almost 700 Pg of carbon, marine dissolved organic carbon (DOC) stores more carbon than all living biomass on Earth combined. However, the controls behind the persistence and the spatial patterns of DOC concentrations on the basin scale remain largely unknown, precluding quantitative assessments of the fate of this large carbon pool in a changing climate. Net removal rates of DOC along the overturning circulation suggest lifetimes of millennia. These net removal rates are in stark contrast to the turnover times of days to weeks of heterotrophic microorganisms, which are the main consumers of organic carbon in the ocean. Here, we present a dynamic “MICrobial DOC” model (MICDOC) with an explicit representation of picoheterotrophs to test whether ecological mechanisms may lead to observed decadal to millennial net removal rates. MICDOC is in line with >40,000 DOC observations. Contrary to other global models, the reactivity of DOC fractions is not prescribed, but emerges from a dynamic feedback between microbes and DOC governed by carbon and macronutrient availability. A colimitation of macronutrients and organic carbon on microbial DOC uptake explains >70% of the global variation of DOC concentrations, and governs characteristic features of its distribution. Here, decadal to millennial net removal rates emerge from microbial processes acting on time scales of days to weeks, suggesting that the temporal variability of the marine DOC inventory may be larger than previously thought. With MICDOC, we provide a foundation for assessing global effects on DOC related to changes in heterotrophic microbial communities in a future ocean.

Climate, fire, and anthropogenic disturbance determine the current global distribution of tropical forest and savanna

Tropical forest and savanna biomes are pivotal in the functioning of the Earth system. Both are biodiverse and under increasing threat due to land clearing and anthropogenic climate change, and play important roles in the global carbon cycle, through maintenance of a large carbon pool in tropical forests, and exchange in savannas through extensive landscape fires. Reliable mapping of tropical forest and savanna is essential to understand how the current distribution of these vegetation types is controlled by climate land clearing and fire. Using Google Maps satellite imagery, we manually classified 24 239 random points as forest, savanna, or anthropogenic landscapes within the tropics and applied this novel dataset to defining the climatic zone where forest and savanna exist as alternative states. Because fire and climate are correlated, we developed separate geospatial models to rank the importance of climate, topography, and human influence on vegetation present. This modeling confirmed that those areas with more fires had lower probabilities of tropical forest, that forest was most likely in areas with high mean annual rainfall with little seasonal variation in precipitation, and that anthropogenic factors disrupt this environmental predictability. We also identified areas where tropical forest and savanna both co-occur, but these were relatively uncommon. These relationships suggest that future drier climates projected under anthropogenic climate change, combined with clearing and burning that have reduced tropical forest extent to a subset of its theoretical distribution, will lead to irreversible loss of tropical forests. Our modeling provides global mapping that can be used track further changes to distribution of tropical forests.

Estimated mangrove carbon stocks and fluxes to inform MRV for REDD+ using a process-based model

Mangrove forests are important due to their strong capability for carbon storage, especially in soils. Understanding carbon dynamics in these forests is fundamental to estimate their roles in carbon storage and mitigating climate change. This study used a process-based model, MCAT-DNDC, to assess mangrove carbon sequestration and fluxes at a 30-m spatial resolution in three African countries, Gabon, Mozambique and Tanzania. The simulated above- and below-ground biomass at inventory plots in each country was approximate to actual observations with mean errors <5% for aboveground biomass and <8% for belowground biomass, indicating that the MCAT-DNDC model can be a useful tool for assessing mangrove carbon storage and fluxes. The results from assessing mangrove carbon storage and fluxes for the three countries showed that the mangroves in these countries are large carbon pools, they export large amounts of dissolved and particulate carbon components to riverine and oceanic ecosystems, and they bury a large amount of carbon in soils. However, soil-borne greenhouse gases CO2, CH4 and N2O fluxes from mangrove forest lands were low. There were large differences in all mangrove carbon components among the mangroves in the three countries, indicating that regional or global mangrove carbon stocks estimated using a process-based model may be better than the extrapolations using limited inventories.

A dataset of soil carbon flux in Xishuangbanna tropical rainforest ecosystem from 2003 to 2008

Long-term and large-scale carbon flux data supports research on key processes of the global carbon and water cycle. Soil is the largest carbon pool in the terrestrial ecosystem. Small variations in soil carbon can significantly impact the carbon budgets of terrestrial ecosystems. Tropical forest is a crucial carbon sink in the world. However, there have been some reports indicating a decrease in soli carbon levels, potentially approaching neutrality, as a result of soil carbon degradation caused by global changing. Thus, the long-term and continuous observation of soil carbon flux is of great significance for evaluating the role of soil carbon in the tropical carbon cycle. ChinaFLUX Observation and Research Network (ChinaFLUX) has not only conducted the observation and research on carbon flux by using vorticity correlation technology, but also carried out the long-term observation and research on surface CO2 flux by using the box method. In this dataset, we collected and sorted out the soil CO2 emission flux data and conventional meteorological data of Xishuangbanna tropical rain forest ecosystem from January 2003 to August 2008 according to the static box observation method and data quality control management of greenhouse gas emissions. The dataset consists of three types of files at daily, monthly and yearly scales, covering surface CO2 flux, pressure, atmospheric temperature, soil temperature (5 cm) and soil water content (5 cm). It is expected to provide essential data for estimating carbon balance and understanding carbon cycle processes at both tropical and global scales.

Contribution of soil aggregate particle size to organic carbon and the effect of land use on its distribution in a typical small watershed on Loess Plateau, China

Soil organic carbon (SOC) is an important pool in the global carbon cycle, playing a vital role in moderating atmospheric CO2 concentrations. Soil is the largest carbon pool in terrestrial ecosystems and, as the basic unit of soil structure, soil aggregates are key to protecting soil carbon pools. However, the influence of soil aggregate particle size, SOC distribution, and the contribution of different particle sizes to SOC is still unclear, particularly under different land use types. In this study, soil samples were collected from five land use types (slope farmland (SF), forest (FL), grassland (GL), shrubland (SL), and terraced field (TF)) in a typical small watershed in the Loess Plateau, China. We analyzed the soil aggregate particle size, composition, and stability after dry and wet sieving, SOC content of different particle sizes in aggregates, and the effects of different aggregate particle sizes and land use on the distribution of SOC. The results showed that, the surface soil (0 ∼ 20 cm) water-stable aggregates were relatively stable, particularly under FL, the mean weight diameter (MWD) value was 2.16 mm. Deep soil (40 ∼ 60 cm) non-water stable aggregates were more stable, and GL was optimal, the MWD value was 3.94 mm. SOC and total nitrogen (TN) were significantly correlated with water-stable aggregate stability indicators (p < 0.01). The SOC content in the small aggregates (0.25 ∼ 2 mm) was the highest and lowest in the microaggregates (<0.25 mm) under different land use. The carbon/nitrogen (C/N) ratio of microaggregates was significantly higher in SF (the C/N ratio was 23.17) and GL (the C/N ratio was 31.04) than in other land uses (p < 0.01) in the 20–40 cm soil layer. In surface soil, small aggregates contributed>50% to SOC in SF and TF. In deep soil, small aggregates under all five land uses made the largest contribution rate to SOC at 57%. These findings indicate that carbon sequestration in the study area can be improved by a combination of appropriate soil management with ecological construction to increase the content of small aggregates, strengthen the fixation and protection of SOC, and reduce carbon emissions in the soil.

Does Spartina invasion affect the carbohydrate assimilation of polychaetes in mangroves? A case study in the Zhangjiang Estuary Mangrove National Nature Reserve

Carbohydrates, which form a large pool of carbon in plant litter, are a major driver of litter dynamics and benthic food webs in Spartina-invaded mangrove ecosystems. However, there are large gaps in knowledge regarding the effects of exotic leaf litter carbohydrates on benthic carbon assimilation. In this study, the dynamics of carbohydrates, including cellulose, lignin and monosaccharides (glucose, mannose, galactose, xylose and arabinose), during the leaf litter decomposition of the exotic Spartina alterniflora (SA) and the native mangrove plant Kandelia obovata (KO) were analyzed and compared. Meanwhile, the differences in the δ13C values of cellulose and lignin were used to analyze the utilization of leaf litter carbohydrates by polychaetes after Spartina invasion. Our results showed that the decomposition rate of exotic SA leaf litter was lower than that of KO leaf litter. The contents of cellulose, glucose and galactose in SA leaf litter were higher than those in KO leaf litter (P < 0.05). The decomposition rates of cellulose and monosaccharides (arabinose, xylose, mannose and galactose) in KO leaf litter were faster than those in SA leaf litter (P < 0.05). However, there was no significant difference in the decomposition rates of lignin in the leaf litter between exotic SA leaf litter and native KO leaf litter (P > 0.05). In the native KO plot, the carbohydrates of KO leaf litter contributed to >55% (cellulose, 26.91–28.02%; lignin, 28.05–28.64%) of the organic carbon sources of polychaetes. However, in the exotic SA plot, the carbohydrates of exotic SA leaf litter contributed to >65% (cellulose, 36.83–38.30%; lignin, 28.47–30.52%) of the organic carbon sources of polychaetes. The obtained results suggested that Spartina invasion increased the assimilation of cellulose by polychaetes. The high content and low decomposition rate of cellulose in the carbohydrates of exotic SA leaf litter might be important mechanisms for the carbon assimilation changes in the benthos of Spartina-invaded mangrove ecosystems. Given that Spartina has colonized in the Zhangjiang Estuary Mangrove Reserve, this study provides a theoretical basis for the recovery of benthos after ecological restoration. Furthermore, the mechanisms of carbohydrate assimilation by different benthos of different ecosystems should be comprehensively considered in the future.

Autochthonous sources and biogeochemical processes drive the spatiotemporal variation of DOM composition and optical indicators in a karst river

Dissolved organic matter (DOM), as a large carbon pool, is key to biogeochemical cycles, especially in some unique areas. Karst rivers typically exhibit unique biogeochemical characteristics and different human inputs; whether and how these special differences affect DOM distribution, transport and composition are not clear. Here, 134 samples were obtained from the Daning River, a typical karst river located in the mountain landscapes of the Yangtze River, during both the dry and wet seasons. Multiple methods including UV–visible absorption, three-dimensional fluorescence spectroscopy parallel factor analysis (PARAFAC), principal component analysis (PCA) and correlation analysis were used to research the molecular compositions, optical properties and origins of DOM. During the wet season, there was higher aromaticity and intensity of UV absorbing functional groups in DOM. The higher Fmax of the protein-like component in the wet season suggested more biological sources in DOM. PCA indicated that autochthonous sources and biodegradation were the main sources of DOM during the two seasons. During the dry season, forest showed the opposite effect on DOM quality when compared to farmland and urban. The findings demonstrated that monsoonal climate and biogeochemical processes were the primary reasons for the differences in DOM components and sources, which benefits the understanding of carbon biogeochemical processes in the karst rivers. More importantly, the analysis of DOM composition and sources can provide valuable insights into identifying potential contaminants and understanding water quality variations, which is beneficial to the concrete implementation of water protection.

Subsea permafrost organic carbon stocks are large and of dominantly low reactivity

Subsea permafrost carbon pools below the Arctic shelf seas are a major unknown in the global carbon cycle. We combine a numerical model of sedimentation and permafrost evolution with simplified carbon turnover to estimate accumulation and microbial decomposition of organic matter on the pan-Arctic shelf over the past four glacial cycles. We find that Arctic shelf permafrost is a globally important long-term carbon sink storing 2822 (1518–4982) Pg OC, double the amount stored in lowland permafrost. Although currently thawing, prior microbial decomposition and organic matter aging limit decomposition rates to less than 48 Tg OC/yr (25–85) constraining emissions due to thaw and suggesting that the large permafrost shelf carbon pool is largely insensitive to thaw. We identify an urgent need to reduce uncertainty in rates of microbial decomposition of organic matter in cold and saline subaquatic environments. Large emissions of methane more likely derive from older and deeper sources than from organic matter in thawing permafrost.

FT-ICR-MS combined with fluorescent spectroscopy reveals the driving mechanism of the spatial variation in molecular composition of DOM in 22 plateau lakes

Dissolved organic matter (DOM) is the largest carbon pool and directly affects the biogeochemistry in lakes. In the current study, fourier transform ion cyclotron mass spectrometry (FT-ICR-MS) combined with fluorescent spectroscopy was used to assess the molecular composition and driving mechanism of DOM in 22 plateau lakes in Mongolia Plateau Lakes Region (MLR), Qinghai Plateau Lakes Region (QLR) and Tibet Plateau Lakes Region (TLR) of China. The limnic dissolved organic carbon (DOC) content ranged from 3.93 to 280.8 mg L−1 and the values in MLR and TLR were significantly higher than that in QLR. The content of lignin was the highest in each lake and showed a gradually decreasing trend from MLR to TLR. Random forest model and structural equation model implied that altitude played an important role in lignin degradation while the contents of total nitrogen (TN) and chlorophyll a (Chl-a) have a great influence on the increase of DOM Shannon index. Our results also suggested that the inspissation of DOC and the promoted endogenous DOM production caused by the inspissation of nutrient resulted in a positive relationship between limnic DOC content and limnic factors such as salinity, alkalinity and nutrient concentration. From MLR to QLR and TLR, the molecular weight and the number of double bonds gradually decreased but the humification index (HIX) also decreased. In addition, from the MLR to the TLR, the proportion of lignin gradually decreased, while the proportion of lipid gradually increased. Both above results suggested that photodegradation was dominated in lakes of TLR, while microbial degradation was dominated in lakes of MLR.

Chemolithotrophic microbiome of buried soil layers following volcanic eruptions: A potential huge carbon sink

Soil burial processes create large pools of soil organic carbon that have been sequestered in the deep for long periods of time. This buried organic carbon is generally thought to be stable and resistant to decomposition, consisting of the remains of past plants and soil microbes. As a result, there is a lack of research on the role of microbiomes in the carbon transformation processes that may occur in the deep buried soil layers. In this study, we characterized the microbiomes and their metabolic potentials in organic soil layers buried following the volcanic eruption approximately 300 and 1,640 years ago, using bacterial and archaeal 16S rRNA gene sequencing. Both buried layers had comparable or even larger carbon and nitrogen contents and a microbial population as abundant and diverse as that of the surface layer. However, the organic soil layer buried 1,640 years ago showed a distinct microbiome from the surface layer, with a higher proportion of chemolithotrophic taxa, such as methanogens and NH3-oxidizing archaea. Additionally, this buried layer had a higher metabolic potential for fixing CO2 and synthesizing organic matter, while the surface community had a higher potential for degrading organic matter. These findings suggest that the buried organic soil in the deep may not simply be an accumulation of past organisms, but rather an active carbon sink driven by a chemolithotrophic microbiome that differs from the organotrophic microbiome of the surface soil.

The interplay between terrestrial organic matter and benthic macrofauna: Framework, synthesis, and perspectives

AbstractEcosystems are shaped by physical, chemical, and biological drivers, which affect the quality and quantity of basal energy sources, with impacts that cascade to higher trophic levels. In coastal, shelf, and marine habitats, terrestrial‐derived organic matter (ter‐OM) can be a key driver of ecosystem structure and function. Climate change is expected to alter land–ocean connectivity in many regions, with a broad range of potential consequences for impacted ecosystems, particularly in the coastal zone. The benthic compartment is an important link between the large organic carbon pools stored on land and the marine environment. At the same time, the macrofauna plays a key role in the processing, biological uptake, and fate of ter‐OM in the aquatic environment, with implications for coastal ecosystem functioning, benthic–pelagic coupling, carbon burial, and biogeochemical cycles. However, information about relationships between land–ocean connectivity (including ter‐OM loads) and coastal benthic community responses remains spread across disciplines, and a broad perspective on the potential impacts of a changing climate is still missing. Here, we explore the interplay between benthic macrofaunal communities and ter‐OM through a paired narrative and research weaving analysis, which combines systematic mapping and bibliometric analysis. The review describes the past development and status of the research field as well as the lack of information in some geographical regions and habitats worldwide. We highlight the role of macrofauna in carbon cycling and the growing evidence that ter‐OM plays a key role in the structure and function of benthic communities, not strictly limited to estuarine habitats. Climate change poses challenges for the prediction of future ter‐OM fluxes and potential macrofauna responses to this additional stressor, thus requiring new methodological approaches (e.g., multimarker approaches for OM characterization) and long‐term monitoring programs across different habitats and spatiotemporal scales.

Heterotrophic aquatic metabolism and sustained carbon dioxide emissions in a mineral-soil wetland restored with treated effluent

Wetlands are economically valuable ecosystems, in part because they purify wastewater by retaining and processing nutrients, organic matter (OM), and other pollutants. While natural wetlands are highly productive and sequester large pools of carbon (C), it is unclear whether the C cycle of restored treatment wetlands is functionally consistent with natural systems. This knowledge gap limits our appreciation for the role that wetland restoration can play as a natural solution to climate change. Here, we quantified metabolic and C cycling patterns of a restored, multi-basin wetland (Frank Lake, Alberta, Canada) receiving municipal and beef processing plant effluents rich in nutrients and OM. We conducted metabolic measurements in all three basins using dissolved oxygen sensors deployed under ice and in open water. Extreme production and respiration indicated that effluent was largely mineralized and replaced with wetland OM in transit. The heterotrophic status of all basins aligned with a published mass budget demonstrating the aquatic habitat of the wetland was an OM sink under current drought conditions that lengthen effluent processing time. Floating chamber measurements in open water zones confirmed that the wetland was a source of CO2 to the atmosphere. From input to outflow, sustained emissions led to declining pCO2 and a decline in the ratio of dissolved inorganic to organic C. Over 30 years post-restoration, the open water habitats in Frank Lake remain heterotrophic and a net source of CO2, suggesting that the trajectory of aquatic C cycling may be distinct from wetlands restored with non-effluent water sources.

Short-Term Effects of Tunnel Construction on Soil Organic Carbon and Enzyme Activity in Shrublands in Eastern Tibet Plateau

Soil is the largest carbon pool, and our understanding of soil organic carbon (SOC) has been enhanced due to its role in mitigating climate change. However, fundamental uncertainty remains about the quantitative importance of tunnel excavation, one of the most common practices for road construction in mountainous areas, on the SOC dynamics. Therefore, the short-term effects of tunnel construction on SOC and its fraction, soil microbial carbon, and soil enzyme activity within 0–20 cm in two shrublands (dominated by Quercus aquifolioides and mixed with Q. aquifolioides, Rhododendron phaeochrysum and Betula platyphylla, respectively) in Eastern Tibet Plateau were investigated. The results showed that, regardless of vegetation type, SOC, dissolved organic carbon, and easily oxidizable carbon were 27.14 ± 2.87, 6.70 ± 0.74, and 0.29 ± 0.10 g kg−1 for tunnel-affected area of Q. aquifolioides and 47.96 ± 17.89, 11.19 ± 2.92, and 0.24 ± 0.04 g kg−1 for the mixture of Q. aquifolioides, R. phaeochrysum, and B. platyphylla, respectively. The values were not significantly different from those of tunnel unaffected areas (p &gt; 0.05). Similarly, soil enzymes (except cellulase) were not significantly different between tunnel-affected and unaffected areas (p &gt; 0.05), indicating that tunnel construction had a minor impact on the SOC fractions and soil enzymes in the early stage. The unchanged SOC and enzyme activities may be associated with no changes in vegetation production and soil water content in tunnel-affected areas. However, vegetation type had a significant impact on SOC and its fractions and soil enzymes (p &lt; 0.05), demonstrating the importance of vegetation control on the SOC fraction and soil enzymes. This study would be one of the earliest studies to explore the effects of tunnel construction on soil carbon dynamics based on field experiment, which could provide a new concept on environmental sustainability during tunnel construction. However, a long-term study is encouraged to detect the effects of tunnel construction SOC and soil enzymes in the future.

Discussion on the Necessity of Soil Organic Carbon Mineralization in Degraded Forest Land in Aeolian Sand Beach Area

Soil organic carbon is the largest carbon pool in terrestrial ecosystem, and its dynamic change is an important factor affecting the carbon budget balance of terrestrial ecosystem and the global carbon cycle. The forest land is extremely sensitive to the change of soil carbon pool. The wind-sand beach area in the north of Jingbian is located at the southern tip of the Mu Us Desert, and the ecological environment is fragile. The shelterbelt has been seriously degraded and fragmented, forming a large area of fragmentated forest, which has gradually changed from the original carbon sink into a carbon source. In addition, this region is dry, less rain and more wind-blown sand, which determines that temperature and moisture are the key factors affecting the mineralization and stability of soil organic carbon in this region. This paper analyzed and summarized the influencing factors of soil organic carbon mineralization, such as water, temperature and organic carbon components, and discussed the necessity of studying soil organic carbon mineralization in degraded forest land.