Large Tropical Reservoir Research Articles

Understanding the Water Quality Dynamics in a Large Tropical Reservoir Under Hydrological Drought Conditions

Understanding the Water Quality Dynamics in a Large Tropical Reservoir Under Hydrological Drought Conditions

Persistence of the non-native Kellicottia bostoniensis (Rousselet, 1908) in a large tropical reservoir

Persistence of the non-native Kellicottia bostoniensis (Rousselet, 1908) in a large tropical reservoir

Multivariate approach to link phytoplankton dynamics to environmental variations in a large tropical reservoir

Multivariate approach to link phytoplankton dynamics to environmental variations in a large tropical reservoir

Effects of atmospheric low-level jets on the mixing process of a large tropical reservoir.

Changes in the physical and biogeochemical properties of water columns are frequently associated with cold fronts and mesoscale convective systems due to increased cloud cover. The effects of low-level jet (LLJ) events on thermal stratification and water quality, however, remain undescribed, particularly for tropical reservoirs. Here, water temperature time series are combined with meteorological data, LIDAR observations, ERA5 reanalysis data, and hydrodynamical modeling to investigate the impact of an event of LLJ over the Furnas hydropower reservoir in Brazil. The LLJ event was characterized by dry, intense, and persistent winds (~10 m s-1) blowing for more than 12 hours over the main fetch of the reservoir. In the downwind side of the lake, the surface mixed layer depth increased by 50% during the LLJ event. The changes to the water column were produced by a combination of wind-induced upwelling, shear-driven mixing, and nocturnal convective overturning, different from the heat balance expected during passing cold fronts and mesoscale convective systems. The results suggest that both momentum and heat fluxes during LLJ events need to be accounted for in lake modelings to reproduce the vertical mixing process.

Infestation with metacercarial stage of Isoparorchis hypselobagri (Billet, 1898) in cage cultured Ompok bimaculatus vis-a-vis host and environmental interaction in a large tropical reservoir

The present study reports the occurrence of metacercariae of Isoparorchis hypselobagri in a food fish Ompok bimaculatus cultured in cages at Maithon reservoir, India. The metacercarial parasite was initially identified morphologically. Molecular identification through partial amplification of 18S rRNA and cytochrome oxidase subunit 1 (COI) gene and sequencing further confirmed the parasite species. The patterns of infection in terms of intensity, mean intensity, and prevalence of metacercariae in O. bimaculatus was recorded. Influence of host size, season and water quality on infection rate was tested through Generalized linear model (GLM). The prevalence varied from 42.2% to 88.8% and intensity of infection ranged from 1 to 10. PCA analysis showed that the metacercarial parasite was mostly located near the air bladder, around kidney and near kidney of the host. GLM results showed that the size of the fish influenced the intensity of infection. Higher water temperature reduced the parasitic growth whereas dissolved oxygen was positively related with intensity of the parasite infection. The occurrence of the first intermediate host snails indicated that they might be the possible source of cercaria in the cages. This study is the first report of I. hypselobagri infections in inland cage culture from India and has implications for understanding the ecological and taxonomic patterns and pathology in caged fish in tropical reservoirs. The high infestation rate and large size of parasite presents serious threat for production of this high value fish species in cages. The presence of parasites not only affects growth and survival but also leads to reduced consumer acceptance. The control of snail vectors might play a significant role in prevention of parasitic diseases in cage culture system. Hence, before selection of candidate fish species and site for cage culture, management of such parasitic disease should be duly considered. This will not only prevent economic losses due to parasitic diseases but also ensure consumer safety.

Cyanophycin accumulated under nitrogen-fluctuating and high-nitrogen conditions facilitates the persistent dominance and blooms of Raphidiopsis raciborskii in tropical waters

Nutrient storage is considered a critical strategy for algal species to adapt to a fluctuating nutrient supply. Luxury phosphorus (P) uptake into storage of polyphosphate extends the duration of cyanobacterial dominance and their blooms under P deficiency. However, it is unclear whether nitrogen (N) storage in the form of cyanophycin supports persistent cyanobacterial dominance or blooms in the tropics where N deficiency commonly occurs in summer. In this study, we examined genes for cyanophycin synthesis and degradation in Raphidiopsis raciborskii, a widespread and dominant cyanobacterium in tropical waters; and detected the cyanophycin accumulation under fluctuating N concentrations and its ecological role in the population dynamics of the species. The genes for cyanophycin synthesis (cphA) and degradation (cphB) were highly conserved in 21 out of 23 Raphidiopsis strains. This suggested that the synthesis and degradation of cyanophycin are evolutionarily conserved to support the proliferation of R. raciborskii in N-fluctuating and/or deficient conditions. Isotope 15N-NaNO3 labeling experiments showed that R. raciborskii QDH7 always commenced to synthesize and accumulate cyanophycin under fluctuating N conditions, regardless of whether exogenous N was deficient. When the NO3−-N concentration exceeded 1.2 mg L−1, R. raciborskii synthesized cyanophycin primarily through uptake of 15N-NaNO3. However, when the NO3−-N concentration was below 1.0 mg L−1, cyanophycin-based N was derived from unlabeled N2, as evidenced by increased dinitrogenase activity. Cells grown under NO3−-N < 1.0 mg L−1 had lower cyanophycin accumulation rates than cells grown under NO3−-N > 1.2 mg L−1. Our field investigation in a large tropical reservoir underscored the association between cyanophycin content and the population dynamics of R. raciborskii. The cyanophycin content was high in N-sufficient (NO3−-N > 0.45 mg L−1) periods, and decreased in N-deficient summer. In summer, R. raciborskii sustained a relatively high biomass and produced few heterocysts (< 1%). These findings indicated that cyanophycin-released N, rather than fixed N, supported persistent R. raciborskii blooms in N-deficient seasons. Our study suggests that the highly adaptive strategy in a N2-fixing cyanobacterial species makes mitigating its bloom more difficult than previously assumed.

Effectiveness of carp seed stocking in large reservoir ecosystems of India

AbstractTropical reservoir ecosystems cover more than 3.51 million ha in India which are the major sources of fish production from inland open waters and supports rich diversity of fish. The present study quantifies fisheries enhancement and evaluates the impact of stocking of Indian major carp fingerlings in six large reservoir ecosystems of India. Analysis of time series data revealed a significant increase (p &lt; 0.05) in fish yield from 12.3 kg ha−1 year−1 in 2004–2005 to 52.4 kg ha−1 year−1 in 2016–2017 due to stocking. The average fish yield of these six reservoirs was estimated to be higher than the national average of 33 kg ha−1 year−1 recorded for large Indian reservoirs. Fingerling stocking had a significant positive impact on fish yield (r = 0.238, p = 0.035). The reservoir water storage level was positively correlated (r = 0.621, p &lt; 0.05) to fish production. The study also shows that the reservoir habitat support 99 finfish species in the central Indian reservoirs of which two species belong to endangered category warranting conservation efforts. This study is the first of its kind on assessing the impact of stocking in large number of tropical reservoirs including the largest reservoir in the country revealing positive effect on fish yield. The fish yield of 85.8 kg ha−1 year−1 obtained in Gandhisagar reservoir (72,300 ha), India revealed that fisheries enhancement is effective and remunerative. The study also highlights the global status of stocking impact in large reservoirs and deliberates issues and suggests management strategies for sustainable fisheries enhancement in large tropical reservoirs.

Environmental parameters and stocking density influence growth, feed utilization and economics of butter catfish, Ompok bimaculatus (Bloch, 1794) production in floating net cages in a large tropical reservoir, India.

An experiment was conducted to study the influence of environmental parameters and stocking density on growth, survival, feed utilization, and economic feasibility of a high value butter catfish, Ompok bimaculatus in floating cages in a large tropical reservoir of India for 180 days. The fingerlings (11.44 ± 1.33 cm; 8.05 ± 3.27 g) were stocked at three stocking densities, viz., 15, 25 and 35 fingerlings m-3 in GI cages (32m3) in triplicates. Commercial floating pellets were fed to fish at 5-3% of fish biomass. The results indicated that the fishes at the lowest stocking density of 15 fingerlings m-3 had significantly higher (p < 0.05) growth in relation to weight gain percentage (717.67 ± 39.10) and specific growth rate (1.14 ± 0.05). Survival percentage was also significantly higher (p < 0.01) at lower stocking densities compared with 35 fingerlings m-3. Similarly, the feed conversion efficiency (0.423 ± 0.025), protein efficiency ratio (1.37 ± 0.15) and feed conversion ratio (FCR) (2.37 ± 0.16) were significantly better at density of 15 fingerlings m-3. The fish growth and feed utilization efficiency did not vary significantly (p > 0.05) between stocking densities of 15 fingerlings m-3 and 25 fingerlings m-3. The condition factor was insignificantly higher at lower densities and its values close to 1 indicated congeniality of reservoir ecosystem for cage culture of the species. The coefficient of variation of weight was significantly higher (24.19 ± 1.20) at 35 fingerlings m-3. The highest economic gains in terms of benefit cost ratio (1.77) were achieved at the lowest stocking density. The present study indicated better growth and economic returns at lower stocking densities of 15-25 fingerlings m-3. The nutrient load and plankton abundance were higher at culture site, however, did not vary significantly from reference sites throughout the culture period. Although most of the environmental parameters showed significant seasonal variations, dissolved oxygen showed significant positive relation (r = 0.86) with the growth of the fish. This is the first study reporting feasibility of cage culture of this highly renumerative species in open waters. The cage culture of this species will not only ensure better economic returns to the marginal cage farmers but will aid in the conservation of this species in natural ecosystem. Being a low volume high value species, the impact on environment will be less compared with high volume low value species. This study will serve as baseline for standardization of its grow-out protocol in cages and will be a step towards much needed species diversification for sustainable small scale cage farming in tropical reservoirs of Asia.

Unaccounted CO2 leaks downstream of a large tropical hydroelectric reservoir

Recent studies show that tropical hydroelectric reservoirs may be responsible for substantial greenhouse gas emissions to the atmosphere, yet emissions from the surface of released water downstream of the dam are poorly characterized if not neglected entirely from most assessments. We found that carbon dioxide (CO2) emission downstream of Kariba Dam (southern Africa) varied widely over different timescales and that accounting for downstream emissions and their fluctuations is critically important to the reservoir carbon budget. Seasonal variation was driven by reservoir stratification and the accumulation of CO2 in hypolimnetic waters, while subdaily variation was driven by hydropeaking events caused by dam operation in response to daily electricity demand. This "carbopeaking" resulted in hourly variations of CO2 emission up to 200% during stratification. Failing to account for seasonal or subdaily variations in downstream carbon emissions could lead to errors of up to 90% when estimating the reservoir's annual emissions. These results demonstrate the critical need to include both limnological seasonality and dam operation at subdaily time steps in the assessment of carbon budgeting of reservoirs and carbon cycling along the aquatic continuum.

Effects of pollution on dormant-stage banks of cladocerans and rotifers in a large tropical reservoir.

Here, we describe the abundance and composition of the dormant-stage banks of cladocerans and rotifers at two locations in a tropical reservoir (Furnas Reservoir, Minas Gerais, Brazil) that are subject to different anthropogenic impacts. We hypothesized that at the site that received sewage, the density of resting eggs would be higher, but the species richness would be lower. Sediment samples were collected monthly at the two sampling stations. We counted the number of dormant stages and performed hatching experiments in the laboratory. Combining both sampling locations, we found a total of 26 species, 16 cladocerans, and 10 rotifers. Our hypothesis was partially corroborated, since the abundance and richness of dormant stages were significantly higher in the location subjected to wastewater release. Environmental pollution resulting from wastewater release and the cultivation of tilapia in cages is the most likely factor contributing to the higher values of egg density at this location. In contrast, the presence of aquatic macrophytes (a possible result of nutrient enrichment) at the same site may have contributed to the increase in species richness. Finally, our study indicates that the wastewater release in the reservoir can affect the production of dormant stages by cladocerans and rotifers.

Evaluating the effectiveness of fingerling stocking and ecological perspectives in enhancing fish harvest in a large tropical reservoir of Northern India

The present study evaluated the effectiveness of stocking the Gangetic carps, viz. Labeo catla, Labeo rohita, and Cirrhinus mrigala, in a large tropical reservoir, Uttar Pradesh, India. Time series data of 22 years (1998 to 2019) on fingerling stocking and fish harvest were collected from the State Department of Fisheries, Govt of Uttar Pradesh. The average annual stocking was 36 nos.ha−1 yr−1, and the fingerling stocking size was ranged between 10 and 90 mm total length (TL) with a mean of 30 mm TL. The average fish production potential of the reservoir was estimated at 77.15 kg ha−1 yr−1. The analysis revealed that fish yield from the reservoir progressively increased from 75.02 t to 297.39 t with a mean of 148.82 ± 69.45 t. The regression analysis between the stocking density and yield of stocked species showed an insignificant relation in the reservoir (R2 = 0.0873, p = 0.2495). The percentage change in the production of stocked species in comparison with the previous year was found to be negative for 12 years between 1998 and 2019 indicated an unstable trend. The finding indicated that low stocking density, very small size at stocking, the low conversion efficiency of the photosynthetic energy to fish (0.016%), and the dominance of catfishes were the major causes for the low fish productivity. We suggest greater stocking density of 500 fingerlings ha−1 yr−1, stocking of fingerlings (> 100 mm TL), in situ breeding and rearing in cages and pens, adoption of cage culture technology, and identification and conservation of the breeding ground of the Gangetic carps to optimize the management regime in utilizing the production potential of reservoirs vis-a-vis ensuring livelihood security and sustainability of the fishers.

Food selectivity and reproductive biology of small indigenous fish Indian river shad, Gudusia chapra (Hamilton, 1822) in a large tropical reservoir.

Assessment of life history strategies of economically important small indigenous fishes (SIF) which are rich in nutritive values is essential and imperative to artisanal and subsistence small-scale inland fisheries of Indian tropical reservoirs. The present investigation aims to estimate the food selectivity in juveniles and adults, reproductive traits such as sexual maturity, gonadosomatic index, ova diameter, and fecundity of Gudusia chapra from a large impounded ecosystem of India. A total of 668 (Juvenile 129, adult 539) specimens, 37 to 142mm standard length and 0.89-25.68g body weight were collected during October 2014-September 2015. The qualitative estimation of the stomach contents revealed herbi-omnivorous feeding behavior of fish. The mean relative gut length (mRGL) of the species was 1.557 ± 0.024 in juveniles and 1.154 ± 0.012 in adults. The sex ratio was 1:1.30 with dominance of females in the population though insignificant. The total length at which 50% individual attain maturity (TL50) was estimated as 80.50mmbased on the logistic curve. The absolute fecundity of the individuals ranged from 749 to15044, and ova diameter ranged from 0.10-2.00mm. The gonadal indices (GSI, MGSI, and DI) indicated that spawning extended throughout the year with peak reproductive activity in October, March-April, and July (mean absolute fecundity ranged from 4000 to 6000). The present study characterized biological and reproductive traits of G. chapra from a large reservoir ecosystem and demonstrated a new trend of reproductive pattern which will serve as benchmark data for future assessment and management planning of this species in impounded waters. The study also suggested some specific management measures for sustainable exploitation and conservation of small indigenous species.

Fatty acids reveal aquaculture and drought effects on a large tropical reservoir

Fatty acids (FAs) and their metrics have been used to detect and assess the impacts of urbanization and agriculture on aquatic ecosystems. Here, we investigated whether seston FAs are also useful to characterize and understand early-stage aquaculture impacts in a large tropical reservoir (Furnas Reservoir, SE Brazil). We tested the hypothesis that single FAs, as well as selected FA metrics in the seston fraction, are efficient markers of net-cage fish farming effects. In general, fish farming had only minor effects on standard water chemical variables, mainly small increases in ammonium, nitrate, and dissolved organic nitrogen concentrations. By increasing concentrations of several polyunsaturated FAs, early-stage fish farming improved sestonic food quality in the more oligotrophic branch of the reservoir under drought conditions. However, in general, increases in concentrations of bacterial FAs, due to fish farming, suggested organic matter (OM) subsidies from non-ingested and non-assimilated fish feed. In the more eutrophic reservoir branch, seston FA profiles suggested that fish farming caused an increase of low-quality food resources, such as cyanobacteria. Thus, background impact levels may determine the biochemical responses of tropical reservoirs to fish farming. Higher contributions of potentially sewage-derived and bacterial FAs during drought conditions, especially at reference sites of the more oligotrophic branch, suggested that drought shifted OM inputs towards anthropogenic sources, thereby overwriting land-use related differences between reservoir branches and homogenizing their environmental conditions. In conclusion, FA variables were useful to evaluate and understand environmental conditions, as well as the effects of early-stage fish farming and drought, and should be considered in impact assessments in tropical lentic ecosystems.

Enhancement of Cloudless Skies Frequency over a Large Tropical Reservoir in Brazil

Several studies show the effects of lake breezes on cloudiness over natural lakes and large rivers, but only few contain information regarding large flooded areas of hydroelectric dams. Most Brazilian hydropower plants have large water reservoirs that may induce significant changes in the local environment. In this work, we describe the prevailing breeze mechanism in a Brazilian tropical hydropower reservoir to assess its impacts on local cloudiness and incoming surface solar irradiation. GOES-16 visible imagery, ISCCP database products, and ground measurement sites operated by INMET and LABREN/INPE provided data for the statistical analysis. We evaluate the cloudiness frequency assuming two distinct perspectives: spatial distribution by comparing cloudiness over the water surface and areas nearby its shores, and time analysis by comparing cloudiness prior and after reservoir completion. We also evaluated the solar irradiance enhancement over the water surface compared to the border and land areas surrounding the hydropower reservoir. The results pointed out daily average cloudiness increases moving away from the reservoir in any of the four cardinal directions. When looking at the afternoon-only cloudiness (14 h to 16 h local time), 4% fewer clouds were observed over the flooded area during summer (DJF). This difference reaches 8% during autumn (MAM) and spring (SON). Consequently, the irradiance enhancement at the water surface compared to external areas was around 1.75% for daily average and 4.59% for the afternoon-only average. Our results suggest that floating solar PV power plants in hydropower reservoirs can be an excellent option to integrate both renewable energy resources into a hybrid power generation due to the high solar irradiance in Brazilian territory combined with the prevailing breeze mechanism in large tropical water reservoirs.

Fluorescence indices of dissolved organic matter as early warning signals of fish farming impacts in a large tropical reservoir

Abstract Dissolved organic matter (DOM) can be an important source of energy and nutrients in aquatic ecosystems and play important roles in carbon and nutrient dynamics of natural and impacted environments. In order to assess the effects of early stage fish farming on dissolved organic carbon (DOC) concentrations as well as DOM fluorescence indices, we took water samples along transects from six fish farms towards reference sites in a large tropical reservoir (Furnas Reservoir, Southeast Brazil) between April 2013 and December 2016. While DOC concentrations did no change along transects, small but significant changes in fluorescence indices were detectable in up to 100 m from fish farms. Higher fluorescence index (FI) values near fish farms pointed to small increases in microbial production due to fish farming. Only in the more pristine of the two main reservoir branches, small differences in the freshness index (β:α) and the peak T/peak C ratio along transects indicated that the relative contribution of recently produced DOM and the biochemical oxygen demand of DOM increased due to aquaculture. The humification index did not respond to fish farming. In summary, current early-stage fish farming did not appear to cause major impacts on DOM quantity and quality, but future increases in net cage and fish farm densities, as well as longer operation times may change this assessment. The fluorescence indices FI, β:α and peak T/peak C ratio may be useful early warning signals for monitoring fish farm impacts in tropical reservoirs.

LENGTH-WEIGHT RELATIONSHIPS OF EIGHTEEN SPECIES OF FRESHWATER FISHES FROM PANCHET RESERVOIR IN GANGES BASIN, JHARKHAND, INDIA

The present study describes the length-weight relationships (LWRs) of 18 fish species from a large tropical reservoir, Panchet, in the Damodar River basin, one of the main tributary of the largest river Ganga in India. A total of 2419 individuals represented by 18 species belonging to 9 families were sampled between November 2014 and June 2016. The b values ranged from 2.469 for Trichogaster chuna to 3.428 for Ailia coila. All the regressions were highly significant (p&lt;0.001). The results revealed positive allometric growth for seven species (b&gt;3, p&lt;0.05), negative allometric growth for seven species (b&lt;3, p&lt;0.05) and isometric growth for four species (b=3, p&gt;0.05). This study represents the first reference on the length-weight relationship of Trichogaster chuna from a reservoir ecosystem. This is the first report on LWRs of five fish species viz., Puntius terio, Pethia conchonius, Sperata seenghala, Ailia coila and Trichogaster chuna from an Indian reservoir. This study provides basic data for future stock assessment studies and management programmes from Panchet Reservoir as well as for complementing the comparisons of LWRs from other ecosystems.

Quantitative Fluxes of the Greenhouse Gases CH4 and CO2 from the Surfaces of Selected Polish Reservoirs

Research carried out in the years 2009–2011 and 2018–2019 sought to determine the magnitudes of fluxes of methane (CH4) and carbon dioxide (CO2) from the surfaces of three eutrophic reservoirs in SE Poland. The “static chamber” method was deployed at five or six stations located along the reservoirs, where the water column at its deepest slightly exceeds 2 m. Obtained values for the fluxes of CH4 varied across a wide (0–2513.48 mmol·m−2·d−1) range, with many of these values therefore exceeding those characteristics for large tropical reservoirs. The reservoirs studied were not found to differ significantly in terms of average CH4 flux, however. Where obtained values for CO2 fluxes in the range from −10.96 to 621.69 mmol·m−2·d−1 were concerned, most fell within the range given for temperate-zone reservoirs, while differences between reservoirs were noted for average values in this case.

Changes in Water and Sediment Quality of a River Being Impounded and Differences Among Functional Zones of the New Large Tropical Hydroelectric Reservoir

Though more large tropical reservoirs are being constructed to meet the increasing energy demand, knowledge on the impact of damming a tropical river and the differences along the river after its impoundment is scarce. Thus, the present study aimed to increase our understanding of such differences both longitudinally and vertically. Water and sediment samples were collected at three different functional zones in the Murum river basin ten months after impoundment began. Results show that the impoundment has an impact on both the water and sediment. Longitudinal variations of water and sediment characteristics were observed at different functional zones. The upstream riverine zone which is subjected to anthropogenic activities serves as the main input of suspended solids and nutrients to the reservoir. The nitrogen changed from a high nitrate and low ammonia condition prior to impoundment to a low nitrate and high ammonia condition due to the accumulation of organic matter and slow nitrification rate after impoundment. In the transitional and lacustrine zones, vertical stratifications of dissolved oxygen and temperature were observed. The thickness of well-oxygenated column for sensitive aquatic organisms was only two meters at some stations. Water quality in all zones showed impairment during the filling phase as compared to the good water quality index before the impoundment. The reduction of suspended solids in the surface water column in the transitional and lacustrine zone is a positive change for aquatic organisms. Sediment characteristics were found to be distinct in the transitional zone where it was lower in sand, higher in silt, clay, organic carbon and nutrients in contrast to the riverine zone. Clay increased in all zones after the impoundment. There is an urgent need to reduce the pollutants input from the upstream riverine zone.

Low input of offshore areas to fisheries in a large tropical reservoir in Lao PDR

Nam Theun 2 is the largest neo-reservoir in Lao PDR that was impounded to produce electricity. The objective of this study was to assess the input of the offshore zone to the total fish production. Hydroacoustics in areas > 5 m deep were sampled during the cold-dry season for 2 consecutive years. Results showed low fish densities (200–300 ind. ha−1), low biomass ( < 1 kg ha−1) and fish of small sizes (approximately 6 cm) in deep zones. Fish stock in the pelagic zone was concentrated between 2 and 13 m depth. The low colonization by fish was corroborated by the small catches achieved by experimental fishing in this part of the reservoir. This study has highlighted the limited contribution of the pelagic zone of a large neo-reservoir to fish production (low density, low biomass and few commercial species) when the reservoir was at its maximal water level. This study suggests that future management actions should mainly focus on the littoral zone.

Temporal and Spatial Study of Water Quality and Trophic Evaluation of a Large Tropical Reservoir

A water quality study was carried out at the Adolfo López Mateos (ALM) reservoir, one of the largest tropical reservoirs in Mexico, located within an intensive agricultural region. In this study, the seasonal and spatial variations of nine water quality parameters were evaluated at four different sites along the reservoir semiannually over a period of seven years (2012–2018), considering the spring (dry) and fall (rainy) seasons. An analysis of variance was performed to compare the mean values of the water quality parameters for the different sampling sites. Then, a multiparametric classification analysis was carried out to estimate the spatial density of the sampling points by using a probabilistic neural network (PNN) classifier. The observations (seasonal and spatial) of the water quality parameters at the ALM reservoir revealed no significant influence. The trophic status was evaluated using the Carlson Modified Trophic State Index, finding the trophic state of the reservoir at the mesotrophic level, with nitrogen being the limiting nutrient. The PNN revealed neural interactions between total suspended solids (TSS) and the other four parameters, indicating that the concentration ranges of five parameters are equally distributed and classified.