The main aim of this paper is to identify critical success factors (CSFs) and investigate whether they are the same or not across different project organization structures. The organization structures under the study are: functional, project, and matrix. The study is based on a survey that was conducted on a large number of software projects in Jordan. To rank success factors (SFs) and identify critical ones, we use the importance index of SFs, which is calculated based on the likelihood and impact across different structures. For deeper analysis, we carry out statistical experiments with an ANOVA test and Spearman’s rank correlation test. Analysis results of an ANOVA test partially indicates that the values of the SF importance index are slightly different across the three organization structures. Moreover, the Spearman’s rank correlation test results show a high degree of correlation of the SF importance index between the function and project organization structures and a low degree of correlation between the function and matrix organization structures.