Large Introductory Biology Course Research Articles

Structure and flexibility: systemic and explicit assignment extensions foster an inclusive learning environment

Many educators strive to create inclusive classrooms where students receive not only knowledge but also empathy from their instructors. When students face unexpected challenges due to illness, academic pressure, or exhaustion, they often seek extensions on assignments. Instructors insert their own biases when they decide who is eligible for an extension. An explicitly communicated penalty-free extension system can eliminate this bias, create an inclusive learning environment, and disinter extension requests from the hidden curriculum. Students used an “extension without penalty” system (EWP) in a large introductory biology course. Mid-semester qualitative data collection helped design an end-of-the-semester quantitative survey about students’ perceived benefits. Assignment submission data, EWP use frequency and grades were directly extracted from the learning management system. Students preferred a two-tier extension system with ideal and extension due dates. The EWP system was used by 78% of the students, but half of them only used it once. Students reported benefits in stress reduction, handling of sickness and emergencies, and improved performance in other courses. Exploratory results indicate there were additional benefits in some areas for first-generation college students. Using the extension due dates did not impact student grades. This study uses evidence to debunk common misconceptions about assignment extensions.

Which course resources and student approaches to learning are related to higher grades in introductory biology?

Undergraduate students in large enrollment gateway courses like introductory biology are often inundated with different study resources to enhance their success on course exams. A good performance on these exams as measured by course grades is a strong determinant of their downstream success in the biology major and ultimately retention in STEM fields. Previous studies have suggested best-practices like pre-class reading guides or Supplemental Instruction. However, many of these studies do not also corroborate student course outcomes with Student Approaches to Learning (SAL). We investigated the association between specific resources and how they correlated with higher exam grades. We further investigated the degree to which SAL also correlated with student outcomes. We collected data over a span of two years from a large introductory biology course (N= 307) from a southeastern university by using a post-semester resource questionnaire. This questionnaire asked the students to describe the study resources they had utilized during the course of the semester and further assessed SAL via the Revised Study Process Questionnaire (R-SPQ-2F). Our results indicate that students who earned a higher overall grade in the course were significantly more likely to self-report higher attendance in optional peer-led Supplemental Instruction meetings and, unexpectedly, were less likely to use pre-class reading guides. These students were more likely to report finding course material interesting, whereas students with lower overall final grades were more likely to report trying to memorize course material. However, we also found that students with higher course grades and high previous college GPAs also reported using rote memorization. Given that the efficacy of resources can vary based on the student, we not only encourage the promotion of resources supported by empirical evidence, but also encourage a deeper understanding of the SAL which shapes resource use.

“It made me feel like a bigger part of the STEM community”: Incorporation of Learning Assistants Enhances Students’ Sense of Belonging in a Large Introductory Biology Course

Large introductory science courses are a particularly important and challenging target for creating inclusive learning environments. In this study, we examined the impact of incorporating learning assistants (LAs) on the learning environment in an introductory biology course taught with two different structures: an in-person lecture with intermittent active-learning components and an online setting taught with a flipped instructional approach. Using a survey that measured sense of belonging in a single class, we found that students in sections with LAs reported greater sense of belonging than students in sections without LAs in both class structures. Further, student focus groups revealed that LAs promoted learning and engagement in the class by answering questions and providing clarity; allowing more use of active- and interactive-learning structures; and serving as accessible, approachable, and immediate sources of help. Student responses also indicated that LAs promoted a sense of belonging in science, technology, engineering, and mathematics (STEM) by decreasing feelings of isolation, serving as inspirational role models, clarifying progression through the STEM educational system, and helping students become more engaged and confident in their STEM-related knowledge and skills. These findings indicate that LAs can support multiple elements of inclusive STEM learning environments.

Biosensors Show Promise as a Measure of Student Engagement in a Large Introductory Biology Course.

This study measured student engagement in real time through the use of skin biosensors, specifically galvanic skin response (GSR), in a large undergraduate lecture classroom. The study was conducted during an intervention in an introductory-level biology course (N = 420) in which one section of the course was taught with active-learning approaches and the other with traditional didactic teaching. GSR results were aligned and correlated with the Classroom Observation Protocol for Undergraduate STEM, or COPUS, and student self-reflections on their own engagement. Results showed that the active-learning section spent more time working in groups, resulting in GSR measures that trended higher and self-reported engagement, while showing indications of higher content learning gains compared with the traditional lecture section. Comparisons between COPUS scores and GSR readings indicate that engagement increased during group work and decreased during listening activities. Throughout a class period, GSR activity of the active-learning group showed increased trends compared with baseline measures, while the traditional lecture group showed decreased trends. Results indicate that GSR is a promising measure of real-time student engagement in the undergraduate classroom, bringing a new technique to discipline-based education researchers who aim to better measure student engagement; however, some limitations exist for broad-scale implementation.

Can Test Anxiety Interventions Alleviate a Gender Gap in an Undergraduate STEM Course?

Gender gaps in exam scores or final grades are common in introductory college science and engineering classrooms, with women underperforming relative to men with the same admission test scores or college grade point averages. After failing to close a historically documented gender gap in a large introductory biology course using interventions targeted at training a growth mindset, we implemented interventions designed to reduce student test anxiety. We combined evidence-based exercises based on expressive writing and on reappraising physiological arousal. We also used a valid measure to quantify test anxiety at the start and end of the course. This instrument measures an individual’s self-declared or perceived test anxiety—also called trait anxiety—but not the immediate or “state” anxiety experienced during an actual exam. Consistent with previous reports in the literature, we found that women in this population declared much higher test anxiety than men and that students who declared higher test anxiety had lower exam scores than students who declared lower test anxiety. Although the test anxiety interventions had no impact on the level of self-declared trait anxiety, they did significantly increase student exam performance. The treatment benefits occurred in both men and women. These data suggest that 1) a combination of interventions based on expressive writing and reappraising physiological arousal can be a relatively easy manner to boost exam performance in a large-enrollment science, technology, engineering, and mathematics (STEM) course and encourage emotion regulation; 2) women are more willing than men to declare that they are anxious about exams, but men and women may actually experience the same level of anxiety during the exam itself; and 3) women are underperforming in STEM courses for reasons other than gender-based differences in mindset or test anxiety.

Introductory Biology in Social Context: The Effects of an Issues-Based Laboratory Course on Biology Student Motivation.

We investigated the effects of a laboratory curriculum developed using the socio-­scientific issues (SSI) framework to contextualize scientific and socially relevant issues for students. Using self-determination theory and hierarchical linear modeling, we examined the effects of the SSI curriculum relative to a control curriculum on student motivation in a large introductory biology course for life science majors. The SSI group had a significant increase in motivation for engaging in the laboratory work relative to motivation of the control group. Additionally, the SSI group showed higher levels of more autonomous forms of regulation concerning participation in laboratory tasks compared with the control group. Interestingly, the SSI-based curriculum seemed to have a buffering effect on typically observed decreases in student motivation over the course of a term. This buffering effect could potentially indicate greater self-determination in students experiencing an SSI-based curriculum, which could lead to greater student success and persistence. Qualitative data suggest that this increased motivation of the SSI group relative to the control group is due to enhanced feelings of relatedness experienced by students, likely due to the SSI.

Measuring Student Motivation in an Introductory Biology Class

Student motivation is widely regarded as an essential prerequisite for learning and success. To learn more about biology student motivation and how it changes over time, pre/post-surveys were administered to a large introductory biology course during the fall of 2015. These pre/post-surveys contained motivation subscales from the Intrinsic Motivation Inventory (IMI) and the Motivated Strategies for Learning Questionnaire (MSLQ). Although students began the course with high levels of motivation, the pre/post-survey scores revealed that their intrinsic motivation, self-efficacy, and value scores declined during the semester. The value/usefulness (IMI), pressure/tension (IMI), and test anxiety (MSLQ) pre-survey scores were the best predictors of course performance. The implications of these findings and suggestions for improving student motivation are discussed.

Investigation of a Stand-Alone Online Learning Module for Cellular Respiration Instruction.

With the recent rise of alternative instructional methodologies such as flipped classrooms and active learning, many core concepts are being introduced outside of the classroom prior to scheduled class meeting times. One popular means for external concept introduction in many undergraduate biology courses is the use of stand-alone online learning modules. Using a group of four large introductory biology course sections, we investigate the use of a stand-alone online learning module developed using animations from Virtual Cell Animation Collection as a resource for the introduction of cellular respiration concepts outside of the classroom. Results from four sections of introductory biology (n = 629) randomized to treatments show that students who interacted with the stand-alone online learning module had significantly higher normalized gain scores on a cellular respiration assessment than students who only attended a traditional lecture as a means of concept introduction (p < 0.001, d = 0.59). These findings suggest a superior ability to convey certain introductory cellular respiration topics in a stand-alone manner outside of the classroom than in a more traditional lecture-based classroom setting.

Exams disadvantage women in introductory biology.

The gender gap in STEM fields has prompted a great deal of discussion, but what factors underlie performance deficits remain poorly understood. We show that female students underperformed on exams compared to their male counterparts across ten large introductory biology course sections in fall 2016 (N > 1500 students). Females also reported higher levels of test anxiety and course-relevant science interest. Results from mediation analyses revealed an intriguing pattern: for female students only, and regardless of their academic standing, test anxiety negatively impacted exam performance, while interest in the course-specific science topics increased exam performance. Thus, instructors seeking equitable classrooms can aim to decrease test anxiety and increase student interest in science course content. We provide strategies for mitigating test anxiety and suggestions for alignment of course content with student interest, with the hope of successfully reimagining the STEM pathway as one that is equally accessible to all.

Implementing an Expressive Writing Intervention for Test Anxiety in a Large College Course.

Test anxiety is a widespread problem that negatively impacts student performance. The mechanism proposed to explain the deleterious effect is that anxious thoughts occupy space in working memory, thus diminishing cognitive capacity. Expressive writing is an intervention shown to decrease the impact of test anxiety. For this intervention, students respond to a prompt directing them to write-down their thoughts and feelings regarding the upcoming exam. To investigate the feasibility of using this intervention in college courses and to discern our students’ thoughts about taking exams, we implemented the intervention in our large introductory biology course. The prompt was placed on the cover page of each exam and students were given five minutes to write. Students were then instructed to rip off, crumple up, and throw the page into the closest aisle and begin the exam. Even though they could not start the exam early, students only spent approximately two minutes writing. Clean up of papers was reasonably accomplished during the exam. Interestingly, crumpling and throwing papers seemed to dissipate tension and created a more relaxed atmosphere in the classroom evidenced by laughing and paper airplanes. We assigned the anonymous student writing into categories (e.g., doodling, pep talks, course content, anxious) as we were interested to see the variation in student responses and monitor if student feelings changed over time. We suggest instructors consider using this intervention to decrease the impact of test anxiety. We found the implementation logistics manageable and reading students’ thoughts made us more empathetic with our students’ experiences.

Students’ Use of Optional Online Reviews and Its Relationship to Summative Assessment Outcomes in Introductory Biology

Retrieval practice has been shown to produce significant enhancements in student learning of course information, but the extent to which students make use of retrieval to learn information on their own is unclear. In the current study, students in a large introductory biology course were provided with optional online review questions that could be accessed as Test questions (requiring students to answer the questions before receiving feedback) or as Read questions (providing students with the question and correct answer up-front). Students more often chose to access the questions as Test compared with Read, and students who used the Test questions scored significantly higher on subsequent exams compared with students who used Read questions or did not access the questions at all. Following an in-class presentation of superior exam performance following use of the Test questions, student use of Test questions increased significantly for the remainder of the term. These results suggest that practice questions can be an effective tool for enhancing student achievement in biology and that informing students about performance-based outcomes coincides with increased use of retrieval practice.

Using Pre-Assessment and In-Class Questions to Change Student Understanding of Molecular Movements.

Understanding how different types of molecules move through cell membranes is a fundamental part of cell biology. To identify and address student misconceptions surrounding molecular movement through cell membranes, we surveyed student understanding on this topic using pre-class questions, in-class clicker questions, and subsequent exam questions in a large introductory biology course. Common misconceptions identified in student responses to the pre-class assessment questions were used to generate distractors for clicker questions. Two-tier diagnostic clicker questions were used to probe incoming common student misconceptions (first tier) and their reasoning (second tier). Two subsequent lectures with assessment clicker questions were used to help students construct a new framework to understand molecular movement through cell membranes. Comparison of pre-assessment and post-assessment (exam) performance showed dramatic improvement in students’ understanding of molecular movement: student answers to exam questions were 74.6% correct with correct reasoning while only 1.3% of the student answers were correct with correct reasoning on the pre-class assessment. Our results show that students’ conceptual understanding of molecular movement through cell membranes progressively increases through discussions of a series of clicker questions and suggest that this clicker-based teaching strategy was highly effective in correcting common student misconceptions on this topic.

A partial flip classroom exercise in a large introductory general biology course increases performance at multiple levels

Incorporation of active learning into large lecture classes is gaining popularity as a pedagogical method due to its known benefits in helping learning outcomes. A more recent active learning technique that has emerged is the flipped classroom. In this study, we investigated the effects of incorporating a ‘partial-flip’ into an introductory general biology course, where only a portion of the class time was spent in a flipped classroom format. This partial flip presented the classic biology experiment of Meselson and Stahl that discovered the mechanism of DNA replication. Performance on in-class formative assessments and subsequent summative assessments (eg out-of-class assignments and exams relating to this material) was compared between a class that had the partial flip and a control class that had a traditional lecture. The partial flip students scored higher on in-class formative questions, specific exam questions and final exam essay questions. We found that the partial flip had different effects in males versus females depending on the assessment. The partial flip manipulation appeared equally effective in aiding both below and above average students in formative assessments. Overall, this study shows that the partial flip classroom can be an effective technique to incorporate into existing courses and that it does provide some benefits compared to traditional lecture.

Presenting Clicker Questions with an Open- Versus Closed-Response Format.

Active learning can improve student learning but can be more difficult to use in large classrooms. Course response systems (clickers) can be used to increase active learning and student discussion. In this study, students in a large introductory biology course were given clicker questions in different formats. Students were first presented with an open response question on a PowerPoint slide where no potential answers were visible. After peer discussion, the same question was presented with potential answers in a multiple choice format and students used their clickers to answer. For comparison, the same questions were asked in a different section of the same course but all questions were in the standard multiple choice format. The results show that C students perform better when required to create their own answer for the question. The instructor also noted that student discussions were longer, most likely because students had to discuss the biology rather than just confirming a specific answer choice.

Differences in metacognitive regulation in introductory biology students: when prompts are not enough.

Strong metacognition skills are associated with learning outcomes and student performance. Metacognition includes metacognitive knowledge-our awareness of our thinking-and metacognitive regulation-how we control our thinking to facilitate learning. In this study, we targeted metacognitive regulation by guiding students through self-evaluation assignments following the first and second exams in a large introductory biology course (n = 245). We coded these assignments for evidence of three key metacognitive-regulation skills: monitoring, evaluating, and planning. We found that nearly all students were willing to take a different approach to studying but showed varying abilities to monitor, evaluate, and plan their learning strategies. Although many students were able to outline a study plan for the second exam that could effectively address issues they identified in preparing for the first exam, only half reported that they followed their plans. Our data suggest that prompting students to use metacognitive-regulation skills is effective for some students, but others need help with metacognitive knowledge to execute the learning strategies they select. Using these results, we propose a continuum of metacognitive regulation in introductory biology students. By refining this model through further study, we aim to more effectively target metacognitive development in undergraduate biology students.

Investigating Issues in the Laboratory

Recent reform initiatives in undergraduate biology call for curricula that prepare students for dealing with real-world issues and making important links between science and society. In response to this call, we have developed an issues-based laboratory module that uses guided inquiry to integrate the concepts of animal behavior and population biology into an issue of both local and global relevance. The issue associated with this module is “What should be done about invasive crayfish?” Students investigate plausible reasons why crayfish are often successful invasive species through hypothesis testing, collection of behavioral data on live crayfish, and quantitative reasoning. Students also consider economic and environmental impacts of invasive species on local and global ecosystems. We implemented this module in a large introductory biology course and conducted survey research to evaluate the module’s potential to serve as an interesting and valuable learning experience for undergraduate biology students.

Writing Assignments with a Metacognitive Component Enhance Learning in a Large Introductory Biology Course.

Writing assignments, including note taking and written recall, should enhance retention of knowledge, whereas analytical writing tasks with metacognitive aspects should enhance higher-order thinking. In this study, we assessed how certain writing-intensive "interventions," such as written exam corrections and peer-reviewed writing assignments using Calibrated Peer Review and including a metacognitive component, improve student learning. We designed and tested the possible benefits of these approaches using control and experimental variables across and between our three-section introductory biology course. Based on assessment, students who corrected exam questions showed significant improvement on postexam assessment compared with their nonparticipating peers. Differences were also observed between students participating in written and discussion-based exercises. Students with low ACT scores benefited equally from written and discussion-based exam corrections, whereas students with midrange to high ACT scores benefited more from written than discussion-based exam corrections. Students scored higher on topics learned via peer-reviewed writing assignments relative to learning in an active classroom discussion or traditional lecture. However, students with low ACT scores (17-23) did not show the same benefit from peer-reviewed written essays as the other students. These changes offer significant student learning benefits with minimal additional effort by the instructors.

A faculty learning community as a vehicle for change: the effect of iterative course change on student learning and attitudes in introductory biology (530.4)

Students learn concepts and skills more efficiently when they actively engage with content, and introductory biology courses nationwide are shifting towards student‐centered classes. However, incorporating student‐centered learning into a large‐enrollment course presents unique challenges. Here we describe a Faculty Learning Community (FLC) where faculty instructors collaborate to transform a large enrollment introductory biology course from a traditional lecture format into a student‐centered course. Activities and strategies are incorporated and improved over time as faculty discuss successes and new approaches, allowing the FLC to optimize activities and build up a resource pool. We evaluate the effect of this iterative change on the classroom environment, student learning, and student attitudes utilizing a pre‐ and ‐post survey design. Preliminary results suggest that student content learning is increasing over time. Further, certain types of activities may be particularly effective at improving learning. Longitudinal data from three semesters of the course will be used to explore 1) how iterative change affects student learning, 2) which approaches are most effective, 3) how to target difficult concepts and 4) how different student populations are impacted by the changes. Results will provide a template for other institutions seeking to incorporate similar course improvements.Grant Funding Source: Supported by HHMI “Engage to Excel” grant at Iowa State University

A Mixed Exam Format Closes the Gap for Students with a Conflict between Their Religious Belief &amp; the Theory of Evolution

We assessed the performance of students with a self-reported conflict between their religious belief and the theory of evolution in two sections of a large introductory biology course (N = 373 students). Student performance was measured through pretest and posttest evolution essays and multiple-choice (MC) questions (evolution-related and non-evolution-related questions) on the final exam and posttest. The two class sections differed only in exam format: MC with or without constructed-response (CR) questions. Although students with a reported conflict scored significantly lower on the final exam in the MC-only section, they scored equally well in the MC+CR section, and all students in the MC+CR section performed significantly better overall. As a result, (1) a religious conflict with evolution can be negatively associated with student achievement in introductory biology, but (2) assessment with constructed response was associated with a closed performance gap between students with and without a conflict. We suggest that differences in exam format and focus on student acceptance of evolution (either evidence-based or opinion), rather than reported conflict, may contribute to the inconsistencies in student learning of evolution across research studies, and that CR questions may help students overcome other obstacles to learning evolution.

The impact of laptop-free zones on student performance and attitudes in large lectures

The goal of this study was to determine if laptop use in lecture negatively impacts learning outcomes of surrounding students taking notes on paper. Two sections of a large introductory biology course (>400 students/section) were zoned into a laptop-permitted and a laptop-free area. Two sections in which laptop users could sit anywhere served as the Control. There was no difference in the attendance (∼85%) or percentage of students using laptops (∼29%) between Zoned and Control sections. Academic performance, based on exam points earned, was not significantly different for paper users in Zoned and Control sections indicating laptop use did not impair the overall achievement of surrounding students. However, there was a correlation between exam performance and note taking preference: paper note takers scored significantly higher and laptop users scored significantly lower than predicted by pre-class academic indicators (p < 0.01, paired t-test). The majority of both laptop (64%) and paper users (82%) in the Zoned sections supported a policy restricting laptop use to specific areas. Thus, while we further investigate whether the relationship between laptop use and performance is correlative or causative, zoning is an effective method for accommodating both laptop users and paper note takers in the same lecture hall.