Potential exists for construction time savings through replacement of cast-in-place substructure columns, piers, or both, with prefabricated bridge elements and systems. Cast-in-place substructure construction requires forming, placing steel, and pouring concrete at the construction site. Using prefabricated elements would decrease the extent of work required at the site and potentially reduce concrete curing time. This research furthers the effort to use ultra-high-performance concrete (UHPC) as a joining material for prefabricated bridge substructure elements. UHPC has a high early strength, requires less development or splice length than conventional concrete, and has been used previously for accelerated construction projects, such as bridge deck replacement with precast units. UHPC also has a discontinuous pore structure that reduces liquid ingress, significantly enhancing durability compared to conventional concrete. Although UHPC has been researched extensively, previous research for reinforcing bar splice and development lengths has focused on #9 and smaller diameter bars. Typically, larger diameter bars are used for substructures. This research determined the required splice lengths for large diameter steel deformed reinforcing bars embedded in UHPC based on 127 individual tests. Three primary variables were included in the testing matrix: bar size, bar spacing, and concrete cover. To achieve at least 75 kips per square inch (ksi) (517 MPa) reinforcing bar stress in UHPC with an early age strength of 14 ksi (97 MPa), the required embedment length ranges from 8 to 13 times the bar diameter and the corresponding splice length varies from 6 to 11 times the bar diameter, depending on the bar size and concrete cover.