First, a quick review of the background: while working in a malaria control campaign in the northern section of the state of Minas Gerais, we came across a large bug locally called ‘‘bar-beiro,’’ which, like the bedbug, infests homes and attacks man at night, when the lights are out. Since this was a blood sucking insect and such insects are of importance in human and animal pathology as vectors of disease, we examined ‘‘barbeiro’’ specimens and found in their hindguts a flagellate of crithidial morphology. The next step was to ascertain whether this flagellate was just a parasite of the insect or a stage in the life cycle of a parasite of vertebrates. In almost all marmoset (Callithrix penicillata) specimens from the same region, we had found a parasitic trypanosome. We suspected that the ‘‘barbeiro’’ was the intermediate host, and the flagellate forms in its gut were stages in the life cycle of this parasite, Trypanosoma minasense Chagas. Because of this, we sent specimens of the bug to our director and teacher Dr. Oswaldo Cruz, who had them feed on a marmoset. Dr. Cruz succeeded in infecting this marmoset with a hemoflagellate which, being quite different in morphology from Trypanosoma minasense, we named Trypanosoma cruzi. I studied the new parasite and found, through repeated experiments, that the insect was indeed the intermediate host, and that it took at least eight days after a bite for transmission to take place. I then made another trip to Minas Gerais for the purpose of establishing the definitive habitual host of the flagellate. The habits of the ‘‘barbeiro’’ suggested a situation of intradomiciliary infection; we were most impressed by the poor state of health of those who lived in infested houses. After conducting physical examinations, we found that the affected persons, especially the children, showed symptoms of a chronic disease unlike those already known and described. These symptoms included some that occur in trypanosomiasis in man and household animals, such as generalized lymph node enlargement, edema, swollen face, etc. We then recalled many patients in that area who had sought me out earlier, sometimes with and sometimes without fever, and these patients always presented the same intense morbid condition and symptoms for which I was then unable to diagnose the cause. Their fever did not yield to quinine, and no malaria parasites could be found in their blood. Our first breakthrough was finding a cat with parasitic hemoflagellates in a house where ‘‘barbeiros’’ were abundant. The first examinations of fresh blood from chronic sufferers were without result. When we were later called to treat a child in grave condition, febrile, with very pronounced swelling in the face, many enlarged nodes in different regions, and thyroid hypotro-phy, we found numerous flagellates in freshly drawn blood. By staining them I was able to identify them as the same parasite transmitted by the ‘‘barbeiro’’ to laboratory animals. Examination of guinea pigs inoculated with blood from this first patient revealed a development of the same process previously observed, and in the lungs we found a schizogony of eight units identical to those observed in our studies of the life cycle of Schizotrypanum cruzi. We obtained more positive results by inoculating guinea pigs with blood from chronic patients. This procedure established the new human-disease entity produced by Schizotrypanum cruzi. We communicated the results of our work to Dr. Oswaldo Cruz, the director of the Manguinhos Institute, who sent a preliminary report to the National Academy of Medicine. Due to other commitments, we could not begin the detailed clinical study of the disease until ten months after these findings. The transmitting insect is a heteropterous hemipteran, species megistus, genus Conorhinus, family Reduvidae (sic). Dr. Arthur Neiva has recently published a detailed study of its biology in the Memorias do Institute Oswaldo Cruz. Conorhinids invade homes, where they actively multiply, becoming exceedingly numerous and troublesome to the occupants. The many cracks in the mud walls of primitive, grassthatched huts are the preferred habitat of the bug, where it greatly multiplies. Even better-built houses, if they offer suitable hiding places, are infested with the bug. The bug can find shelter in the cellars of houses, and enters occupied rooms at night through cracks in the floor. On small farms in the region, we found Conorhinids in different outbuildings such as carriage-houses, storerooms, stables, etc. The bug Source: Carlos Chagas: Coletânea de Trabalhos Cientificos. Brasilia, Editora Universidade de Brasilia, 1981. English version in The Challenge of Epidemiology: issues and Selected Readings. Pan American Health Organization, 1988. Published by Oxford University Press on behalf of the International Epidemiological Association
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