Polymer adhesives are known to exhibit time dependent mechanical behaviour, that becomes more and more evident approaching the glass transition temperature, Tg. When subjected to fatigue, bonded joint lifetime can be therefore affected by the loading frequency and amplitude. According to the literature, the influence of the frequency on the fatigue behavior manifests as a rise of the temperature in the adhesive due to the hysteretic heating, which is related in turn to Tg and to the viscoelastic properties of the adhesive. In this paper, cyclic loading was applied to single lap shear joints until failure at frequencies in the range of 70–90 Hz, that are little explored by the existing literature. The aim is twofold: on one side, to investigate the possibility to speed up fatigue tests in comparison to tests performed in the range of 7–9 Hz; on the other side, to explore the possibility to generate stress-life data in a range of number of cycles between 106 and 108, therefore two order of magnitude higher then usual, without exceeding with the time required for the tests. The joints were made with both aluminum and stainless-steel substrates bonded with a structural epoxy adhesive, to manufacture a Single Lap Joint (SLJ). Two loading ratios R (respectively 0.1 and 0.4, defined as the minimum over the maximum force of the fatigue cycle) and different load ranges were applied. Digital Image Correlation (DIC) technique was used to assess the presence of creep strains. A preliminary investigation consisting in a Dynamic Mechanical Analysis (DMA) of bulk adhesive for the range of frequency of interest, and in the measurement of the temperature of the adhesive layer during some selected fatigue tests was carried out. No significant changes of the viscoelastic properties of the adhesive were found for the frequency of interest, and, at the same time, temperature measurements revealed that the temperature increased by a few degrees, remaining in any case far from the glass transition temperature. The test results showed that only small effect due to the application of a high frequency cyclic loading on the fatigue life apparently occurs for tests carried out at the lowest load ratio, in particular when the applied loads were relatively low, and the number of cycles at failure relatively high. On the opposite, the results of tests carried out at the highest load ratio are affected by the loading frequency, and it has been related to the presence of significant creep deformation within the adhesive layer.