Understanding how species persist in urban landscapes can provide insights into the fate of biodiversityaffected by rapid and unplanned urbanization. While the ecological and conservation implications of urbanization havebeen reasonably well studied for temperate species, relatively little is known for those in tropical latitudes, especiallyNeotropical lizards. We investigated the life history, habitat use, and reproductive seasonality of Green Iguanas (Iguanaiguana) inhabiting an urban area in Colombia. Our analysis of age structure showed that the sex ratio in this populationwas approximately 1:1 at birth and adulthood, suggesting that both sexes are experiencing similar selective pressuresover time. The presence of individuals of all size classes throughout the year is indicative of a stable population. Densityestimates based on linear transect data indicated that iguanas in the studied population occur at higher densities thanthose in rural areas. This increased density might be related to some benefits of urbanization, including human trophicsubsidies and decreased predation and competence. The estimated growth rate was also higher than those reported forpopulations in natural habitats, likely reflecting increased food availability. Iguanas in the study area apparently reproducetwice per year, a pattern that might be explained by rainfall regime. Occupancy modeling revealed that contraryto rural populations, urban Green Iguanas successfully exploit open grassy areas for feeding and basking, with tree coverand built-up areas being key predictors of their occurrence. The ability of urban iguanas to modify some traits of theirbasic ecology to cope with the challenges imposed by urbanization may be crucial for this species to persist or eventhrive in cityscapes.