Collection Of Landraces Research Articles

Genetic variability for end-use quality proteins in a collection of bread wheat Mediterranean landraces

This study explored the natural allelic variation of high molecular weight glutenin subunits (HMW-GS), puroindolines, and wheat bread making (WBM) gene in Mediterranean bread wheat landraces.Within the GLU-1 loci, a rich polymorphism was detected with thirty-five different subunits identified, four at GLU-A1 locus, twenty-one at GLU-B1 locus and ten in GLU-D1 locus, being the predominant subunits combinations 2*, 20x+20y, and 2 + 12, respectively. Null alleles were detected in GLU-B1-1, GLU-B1-2 and GLU-D1-2 genes and rare combinations were observed at low frequencies. The influence of HMW-GS on quality traits was confirmed. Seven different purindoline genotypes were identified in the collection, including two novel mutations at the PINB-D1 gene. The relationship between genotype and grain hardness was not readily apparent, revealing the intricate and multifaceted nature of this trait. The Screening for WBM gene (GWseqVar3 sequence variant) showed presence in few landraces and was correlated with higher loaf volumes (LV) and gluten strength (W).The characterization presented in this work, provides promising targets for further research and breeding efforts to develop wheat varieties with improved end-use quality and adaptability to Mediterranean environments.

Genotype by environment interactions and stability for grain yield and other agronomic traits in selected sorghum genotypes in Ethiopia

AbstractEnvironmental changes pose major impacts on the performance of crop genotypes with important implications for crop improvement strategies. Hence, breeders pay attention to the effects of genotype by environment interaction (GEI) to mine genetic resources and select adapted genotypes. Twenty sorghum genotypes selected from a large collection of Ethiopian sorghum landraces and two improved varieties were evaluated using a randomized complete block design with three replications at eight locations representing different environmental conditions in Ethiopia. The study aimed at assessing GEI and identifying stable and high‐yielding genotypes of sorghum for grain yield and major agronomic traits. Analysis of variance and additive main effect and multiplicative interaction (AMMI) revealed highly significant (p ≤ 0.001) variance due to genotypes, environments, and GEI among all traits except for days to maturity. Plant height, days to maturity, panicle width, panicle weight, and grain yield were highly affected by environment and GEI, while days to flowering, panicle length, and 1000‐grain weight were mainly affected by genotypic variations. The data also suggest the importance of considering GEI in screening for high‐yielding and stable sorghum genotypes across environments. Among testing sites, Chawaka, Gute, and Uke were ideal environments for grain yield and Asosa was the most discriminative environment. Three genotypes (ETSL100808, Merera, and ETSL100474) were superior and stable across test environments for grain yield and related traits. Overall, based on mean grain yield and disease reaction, AMMI, GGE (genotype and genotype by environment interaction) biplot, and regression models, ETSL100808 was the most stable, high‐yielding, and disease‐tolerant sorghum genotype, suggesting its potential both in breeding program, as donor of traits, and for direct release as a variety.

Genetic diversity and population structure of wheat landraces in Southern Winter Wheat Region of China

BackgroundWheat landraces are considered a valuable source of genetic diversity for breeding programs. It is useful to evaluate the genetic diversity in breeding studies such as marker-assisted selection (MAS), genome-wide association studies (GWAS), and genomic selection. In addition, constructing a core germplasm set that represents the genetic diversity of the entire variety set is of great significance for the efficient conservation and utilization of wheat landrace germplasms.ResultsTo understand the genetic diversity in wheat landrace, 2,023 accessions in the Jiangsu Provincial Crop Germplasm Resource Bank were used to explore the molecular diversity and population structure using the Illumina 15 K single nucleotide polymorphism (SNP) chip. These accessions were divided into five subpopulations based on population structure, principal coordinate and kinship analysis. A significant variation was found within and among the subpopulations based on the molecular variance analysis (AMOVA). Subpopulation 3 showed more genetic variability based on the different allelic patterns (Na, Ne and I). The M strategy as implemented in MStratv 4.1 software was used to construct the representative core collection. A core collection with a total of 311 accessions (15.37%) was selected from the entire landrace germplasm based on genotype and 12 different phenotypic traits. Compared to the initial landrace collections, the core collection displayed higher gene diversity (0.31) and polymorphism information content (PIC) (0.25), and represented almost all phenotypic variation.ConclusionsA core collection comprising 311 accessions containing 100% of the genetic variation in the initial population was developed. This collection provides a germplasm base for effective management, conservation, and utilization of the variation in the original set.

Discovering New QTNs and Candidate Genes Associated with Rice-Grain-Related Traits within a Collection of Northeast Core Set and Rice Landraces.

Grain-related traits are pivotal in rice cultivation, influencing yield and consumer preference. The complex inheritance of these traits, involving multiple alleles contributing to their expression, poses challenges in breeding. To address these challenges, a multi-locus genome-wide association study (ML-GWAS) utilizing 35,286 high-quality single-nucleotide polymorphisms (SNPs) was conducted. Our study utilized an association panel comprising 483 rice genotypes sourced from a northeast core set and a landraces set collected from various regions in India. Forty quantitative trait nucleotides (QTNs) were identified, associated with four grain-related traits: grain length (GL), grain width (GW), grain aroma (Aro), and length-width ratio (LWR). Notably, 16 QTNs were simultaneously identified using two ML-GWAS methods, distributed across multiple chromosomes. Nearly 258 genes were found near the 16 significant QTNs. Gene annotation study revealed that sixty of these genes exhibited elevated expression levels in specific tissues and were implicated in pathways influencing grain quality. Gene ontology (GO), trait ontology (TO), and enrichment analysis pinpointed 60 candidate genes (CGs) enriched in relevant GO terms. Among them, LOC_Os05g06470, LOC_Os06g06080, LOC_Os08g43470, and LOC_Os03g53110 were confirmed as key contributors to GL, GW, Aro, and LWR. Insights from QTNs and CGs illuminate rice trait regulation and genetic connections, offering potential targets for future studies.

On-farm crop diversity, conservation, importance and value: a case study of landraces from Western Ghats of Karnataka, India

Landraces are important genetic resources that have a significant role in maintaining the long-term sustainability of traditional agro-ecosystems, food, nutrition, and livelihood security. In an effort to document landraces in the on-farm conservation context, Central Western Ghat region in India was surveyed. A total of 671 landraces belonging to 60 crops were recorded from 24 sites. The custodian farmers were found to conserve a variety of crops including vegetables, cereals and pulses, perennial fruits, spices, tuber and plantation crops. The survey indicated a difference in the prevalence of landraces across the sites. A significant difference with respect to the Shannon-diversity index, Gini-Simpson index, evenness, species richness, and abundance was observed among the different survey sites. Computation of a prevalence index indicated the need for immediate intervention in the form of collecting and ex situ conservation of landraces of some crops as a back-up to on-farm conservation. The study also identified the critical determinants of on-farm conservation, including (i) suitability to regional conditions, (ii) relevance in regional cuisine and local medicinal practices, (iii) cultural and traditional significance, and (iv) economic advantage. The information documented in this study is expected to promote the collection and conservation of landraces ex situ. The National Genebank housed at ICAR-NBPGR, New Delhi conserves around 550 accessions of landraces collected from the Central Western Ghats region surveyed in this report. Information collected from custodian farmers on specific uses will be helpful to enhance the utilization of these accessions.

Assessing phenotypic diversity between different landraces of okra (Abelmoschus esculentus L.)

The objective of the present study was to investigate the morphological variations among collections of okra landraces in order to improve local varieties in Palestine. Eighteen okra accessions from various locations in the West-Bank region were evaluated for morphological variation. Different sixteen morphological traits were used for okra collection evaluation. The results revealed significant differences in seed color, leaf rib color, stem color, branching patterns, and fruit characteristics. Cluster and similarity analysis classified into two main clusters (I and II) with further sub-clusters. Genetic proximity did not strictly align with geographical boundaries, suggesting that factors beyond location influence genetic similarity. Cluster I exhibited greater diversity, with sub-clusters hinting at potential local adaptation. In contrast, Cluster II was smaller and less diverse, implying potentially distinct genetic origins. These findings provide insights for breeding strategies and germplasm conservation.

Phenotypic characterisation and evaluation of resistance to Fusarium ear rot, fumonisin contamination and agronomic traits in a collection of maize landraces

Context Maize is a major crop in Italy and is constantly affected by the fungus Fusarium verticillioides, producing ear rot and grain contamination by fumonisins. Finding new genotypes resistant to Fusarium infection is an important goal for the improvement of maize cultivation. Aims The objective of this work was to test a collection of 33 traditional landraces from the Emilia-Romagna (Italy) region for Fusarium ear rot (FER) severity, fumonisin content, and their agronomic performance. Methods Primary ears were artificially inoculated with a toxigenic strain of F. verticillioides in a 2-year experimental trial. The landrace ‘Nostrano di Storo’ and a commercial hybrid of FAO maturity class 300 were also included and used as comparisons representing a well-known and highly valued landrace and a modern flint hybrid, respectively. Key results The collection showed great phenotypic variability for all the agronomic traits assessed and responded differently to the Fusarium infection with percentages of FER ranging from 6.6% to 49.3%, and fumonisins from 4.3 mg/kg to 34.5 mg/kg. Thirteen and six landraces displayed FER percentages and fumonisin content very similar to the hybrid, respectively. Moreover, eight landraces exhibited grain yield values comparable to the hybrid. Interestingly, Va221, Va227 and EMR03 showed the best combination among these three traits. Conclusions This local material can be considered suitable for breeding purposes targeting the development of FER and fumonisin resistant germplasm. Implications The collection may represent a resource for future research aimed at evaluating the response to multiple pathogens and their associated mycotoxins.

Diversity, conservation and utilization of grain legumes: A potential source for food and nutritional security in Nepal

Grain legumes are precious crops of Nepal in terms of the dietary protein supplement ample concentration of micronutrients, and role in crop intensification and ameliorating soil fertility. About 11% of the cultivated area is occupied by grain legumes that include lentil, chickpea, grasspea, fieldpea, fababean, pigeonpea, soybean, blackgram, horsegram, ricebean, cowpea, and mungbean. About 100 genera and 379 species of legumes are distributed in diverse agroecological zones ranging from terai to the alpine region of Nepal, with growth habits ranging from annual to perennial shrubs. Out of the 379 species of legumes which include grain, vegetable, and forage legumes, 262 are native, and 20 species belonging to the sub-group Papilionacae are used as food legumes. Highest food legume species diversity has been recorded in Macrotyloma (34 spp.), followed by Crotalaria (18 spp.), Vigna (15 spp.), Lathyrus (7 spp.), Vicia (6 spp.), Cajanus (5 spp.), Trigonella (5 spp.) and Phaseolus (4 spp.). Other genus having 1-3 species includes Cicer (3 spp.), Mukuna (3 spp.), Glycine (2 spp.), Canavila (2 spp.), Pisum (2 spp.), Lablab (2 spp.), Pachyrrhizus (1 sp.), Psophocarpus (1 sp.), Lens (1 sp.) and Cyamopsis (1 sp.). Research on grain legumes was initiated in 1976 and a systematic collection of grain legume landraces was done in 1987 by the Grain Legumes Research Program (GLRP). Past collection missions organized in collaboration with the International Development Research Centre (IDRC), the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), the International Center for Agricultural Research in the Dry Areas (ICARDA), and other institutes have resulted in the collection of 1107 landraces and provided an opportunity for their inclusion in the variety improvement program. National Agricultural Genetic Resources Center (NAGRC) has conserved 2936 landraces of different legumes, 951 landraces have been kept in gene banks of ICARDA center, while GLRP Khajura has active collections of 218 landraces and 627 exotic germplasm of various legume crops. Varietal improvement programs resulted in the development of 51 varieties, while 6 varietes were registered and two varieties were de-notified in food legumes (lentil, chickpea, soybean, cowpea, black gram, mungbean, pigeon pea, rice bean, faba bean, grass pea, and kidney bean).

Biochemical Characterization of the Seed Quality of a Collection of White Lupin Landraces from Southern Italy

Lupin species provide essential nutrients and bioactive compounds. Within pulses, they have one of the highest contents of proteins and fibers and are among the poorest in carbohydrates. The Mediterranean region is an important cradle area of the origin and domestication of cultivated white lupin (Lupinus albus L.). In this work, we present the characterization of 19 white lupin landraces collected from several sites in southern Italy, characterized by different pedoclimatic conditions. The protein contents and electrophoretic patterns, total polyphenols, phytic acid, lipids and phosphorous content, and reducing and anti-tryptic activities have been determined for each landrace. The relationships of the compositional characteristics, the area of origin of landraces and between compositional characteristics and thermo-pluviometric trends that occurred in the genotype comparison field during the two-year period between 2019 and 2020 are compared and discussed. From a nutritional point of view, some of the analyzed landraces differ from the commercial reference. The panel of molecular analyses performed can help in building an identity card for the grain to rapidly identify those varieties with the desired characteristics.

Fruit quality and shelf-life of Sardinian tomato (Solanum lycopersicum L.) landraces

The conservation and characterization of landraces have key roles in the safeguarding and valorization of agrobiodiversity. Indeed, these plant genetic resources represent an important crop heritage with quality and sensory characteristics that can be of great use to consumers and industry. In addition, the preservation of genetic resources from the risk of progressive genetic erosion, and the enhancement of their potential can contribute to food security and improve the nutritional value of food. Accordingly, this study aimed to investigate a collection of Sardinian tomato landraces for parameters that have determinant roles in evaluating their responses to conservation, and therefore to consumer acceptance. Six Sardinian landraces and two commercial varieties were cultivated in a two-years off-season trial, harvested at two different maturity stages (turning, red-ripe) and characterized using 14 fruit-related quality parameters that define the marketability, nutritional value, and flavor of the fruit. Data were collected at intervals of 10 days, starting from the harvest date and over 30 days of storage under refrigeration. The simultaneous analysis of all the qualitative characteristics for the different genotypes allowed to clearly differentiate the local varieties from the commercial varieties and a few landraces emerged for their satisfactory performances, e.g. “Tamatta kaki” ad "Tamatta groga de appiccai". In particular, the “Tamatta groga de appiccai” showed satisfactory lycopene content at marketable stages (average 5.65 mg 100g-1 FF), a peculiar orange-pink color with the highest hue angle values (range: H°T0 = 72.55—H°T30 = 48.26), and the highest firmness among the landraces of the red-ripe group (range: EpT0 = 1.64—EpT30 = 0.54 N mm-1). These results highlight the potential of some of the Sardinian tomato landraces for developing new varieties or promoting their direct valorization in local markets and could considerably increase the effectiveness and efficiency of agrobiodiversity conservation strategies.

Collection of cassava landraces and associated farmers’ knowledge, genetic diversity and viral incidence assessment in western Kenya

Collection of cassava landraces and associated farmers’ knowledge, genetic diversity and viral incidence assessment in western Kenya

Genome-wide association analysis of tan spot disease resistance in durum wheat accessions from Tunisia.

Background: Tunisia harbors a rich collection of unexploited durum wheat landraces (Triticum durum ssp. durum) that have been gradually replaced by elite cultivars since the 1970s. These landraces represent an important potential source for broadening the genetic background of elite durum wheat cultivars and for the introgression of novel genes for key traits, including disease resistance, into these cultivars. Methods: In this study, single nucleotide polymorphism (SNP) markers were used to investigate the genetic diversity and population structure of a core collection of 235 durum wheat accessions consisting mainly of landraces. The high phenotypic and genetic diversity of the fungal pathogen Pyrenophora tritici-repentis (cause of tan spot disease of wheat) in Tunisia allowed the assessment of the accessions for tan spot resistance at the adult plant stage under field conditions over three cropping seasons. A genome-wide association study (GWAS) was performed using a 90k SNP array. Results: Bayesian population structure analysis with 9191 polymorphic SNP markers classified the accessions into two groups, where groups 1 and 2 included 49.79% and 31.49% of the accessions, respectively, while the remaining 18.72% were admixtures. Principal coordinate analysis, the unweighted pair group method with arithmetic mean and the neighbor-joining method clustered the accessions into three to five groups. Analysis of molecular variance indicated that 76% of the genetic variation was among individuals and 23% was between individuals. Genome-wide association analyses identified 26 SNPs associated with tan spot resistance and explained between 8.1% to 20.2% of the phenotypic variation. The SNPs were located on chromosomes 1B (1 SNP), 2B (4 SNPs), 3A (2 SNPs), 3B (2 SNPs), 4A (2 SNPs), 4B (1 SNP), 5A (2 SNPs), 5B (4 SNPs), 6A (5 SNPs), 6B (2 SNPs), and 7B (1 SNP). Four markers, one on each of chromosomes 1B, and 5A, and two on 5B, coincided with previously reported SNPs for tan spot resistance, while the remaining SNPs were either novel markers or closely related to previously reported SNPs. Eight durum wheat accessions were identified as possible novel sources of tan spot resistance that could be introgressed into elite cultivars. Conclusion: The results highlighted the significance of chromosomes 2B, 5B, and 6A as genomic regions associated with tan spot resistance.

Genome-wide association analysis of Monilinia fructicola lesion in a collection of Spanish peach landraces

Brown rot, caused by the Monilinia spp., is the disease that causes the greatest losses in stone fruit worldwide. Currently, M. fructicola has become the dominant species in the main peach production area in Spain. The fruit cuticle is the first barrier of protection against external aggressions and may have a key role in the susceptibility to brown rot. However, information on the role of skin fruit on the resistance to brown rot in peach is scarce. Previous genetic analyses in peach have demonstrated that brown rot resistance is a complex and quantitative trait in which different fruit parts and resistance mechanisms are involved. To search for genomic areas involved in the control of the cultivar susceptibility to brown rot and to elucidate the role of fruit skin against this infection, we have studied, for two consecutive seasons (2019 and 2020), the fruit susceptibility to M. fructicola, together with fruit cuticle thickness (CT) and density (CD), in a collection of 80 Spanish and 5 foreign peach cultivars from the National Peach Collection at CITA (Zaragoza, Spain). Brown rot incidence, lesion diameter, and severity index were calculated after 5 days of inoculation on non-wounded fruit. The peach collection has also been genotyped using the new peach SNP chip (9 + 9K). Genotypic and phenotypic data have been used to perform a genome-wide association analysis (GWAS). Phenotyping has shown a wide variability on the brown rot susceptibility within the Spanish germplasm as well as on CD and CT. The GWAS results have identified several significant SNPs associated with disease severity index (DSI), CD, and CT, five of which were considered as reliable SNP-trait associations. A wide protein network analysis, using 127 genes within the regions of the reliable SNPs and previously identified candidate genes (169) associated with Monilinia spp. resistance, highlighted several genes involved in classical hypersensitive response (HR), genes related to wax layers as ceramidases and lignin precursors catalyzers, and a possible role of autophagy during brown rot infection. This work adds relevant information on the complexity resistance mechanisms to brown rot infection in peach fruits and the genetics behind them.

Genetic potential of grain-related traits in rice landraces: phenomics and multi-locus association analyses.

Grain length, width, weight, and the number of grains per panicle are crucial determinants contributing to yield in cereal crops. Understanding the genetic basis of grain-related traits has been the main research object in crop science. Kerala has a collection of different rice landraces. Characterization of these valuable genetic resources for 39 distinct agro-morphological traits was carried out in two seasons from 2017 to 2019 directly in farmers field. Most characteristics were polymorphic except ligule shape, leaf angle, and panicle axis. The results of principal component analysis implied that leaf length, plant height, culm length, flag leaf length, and grain-related traits were the principal discriminatory characteristics of rice landraces. For identifying the genetic basis of key grain traits of rice, three multi locus GWAS models were performed based on 1,47,994 SNPs in 73 rice accessions. As a result, 48 quantitative trait nucleotides (QTNs) were identified to be associated with these traits. After characterization of their function and expression, 15 significant candidate genes involved in regulating grain width, number of grains per panicle, and yield were identified. The detected QTNs and candidate genes in this study could be further used for marker-assisted high-quality breeding of rice.

Back to the future: Using ancient Bere barley landraces for a sustainable future

Societal Impact StatementBere is an ancient barley (Hordeum vulgare L.) that was once widely grown in northern Britain, where its ability to grow on poor soils and under challenging climatic conditions made it a valuable staple. By the end of the 20th century, Bere had largely been replaced by higher‐yielding modern varieties and only survived in cultivation on a few Scottish islands. This article reviews the recent revival of Bere, driven by its use in high‐value food and drink products and multidisciplinary research into its genetics, valuable sustainability traits and potential for developing resilient barley varieties.SummaryIn Britain, modern cereal varieties have mostly replaced landraces. A remarkable exception occurs on several Scottish islands where Bere, an ancient 6‐row barley (Hordeum vulgare L.), is grown as a monocrop or in mixtures. In the Outer Hebrides, the mixture is grown for animal feed, and cultivating it with traditional practices is integral to the conservation of Machair, an important coastal dune ecosystem. In Orkney, Bere is grown as a monocrop, and in situ conservation has recently been strengthened by improved agronomy and new markets for grain to produce unique foods and beverages from beremeal (flour) and malt. In parallel, a recently assembled collection of British and North European barley landraces has allowed the genotypic and phenotypic characterisation of Bere and several associated multidisciplinary studies. Genotyping demonstrated Bere's unique identity compared with most other barleys in the collection, indicating an earlier introduction to Scotland than the Norse settlement (c. 9th century AD) suggested previously. Valuable traits found in some Bere accessions include disease resistance, an early heading date (reflecting a short period from sowing to harvest), the ability to grow on marginal, high pH soils deficient in manganese and tolerance to salinity stress. These traits would have been important in the past for grain production under the region's challenging soil and Atlantic‐maritime climatic conditions. We discuss these results within the context of Bere as a genetic, heritage and commercial resource and as a future source of sustainability traits for barley improvement.

GENETIC ANALYSIS AND MOLECULAR CHARACTERIZATION OF THE WHEATGRASS (AGROPYRON CRISTATUM L. GAERTN.) IN SOUTH-EAST KAZAKHSTAN

Assembly, genetic analysis, and preservation of wheatgrass (Agropyron cristatum L. Gaertn.) diversity is an important task in improving the said crop under the environmental conditions of the Republic of Kazakhstan. The presented study evaluated and isolated various wheatgrass species and their populations for breeding and developing promising cultivars based on the genetic principles to improve the degraded pasture situation in South-East Kazakhstan from the perspective of global climate change. For the succeeding investigations, collection of the wheatgrass landraces from various regions of Kazakhstan ensued, as well as from accessions already conserved in the gene pool, and gained evaluation under the environmental conditions of South-East Kazakhstan. In performance-based screening, 20 wheatgrass cultivars identified underwent further genotyping by using inter-simple sequence repeat (ISSRs) polymerase chain reaction markers. The pertinent study significantly contributes to understanding the Agropyron species’ genetic structure and molecular characteristics. Using seven ISSR primers helped study the kinship and genetic polymorphism of the five Agropyron species collected from diverse locations. The ISSR primers were extremely informative for determining the Agropyron’s genetic diversity and interspecific similarity. Moreover, they come highly recommended for an in-depth study comparing the DNA sequencing of the Agropyron populations available in the National Center for Biotechnology’s database.

Analysis of Genetic Diversity and Development of A Core Collection in Walnut (Juglans regia L.) Germplasm Native to China via Genotyping-by-Sequencing

Popular knowledge of the population structure and genetic diversity of a plant species is essential for designing improvement strategies. The genotyping-by-sequencing (GBS) approach has been used to simplify complex genomes and has become a popular high-throughput molecular tool for selecting and breeding many crop plants, including those with large genomes. This study aimed to construct a core collection of walnut (Juglans regia) germplasm using the GBS approach. A diversity panel of 87 walnut initial genotypes, including 25 landraces, 12 cultivars, and 50 seedling populations, mostly native to the Gansu Province of China, was subjected to GBS. A total of 110,497 high-quality SNPs were identified and used for determining distinct clusters and an optimum number of subpopulations. Structure analysis divided the genotypes into three distinct groups, which coincided with their collection site and year, suggesting a certain degree of separation in the geographical origin and pedigree among the three groups. To maximize germplasm utilization, the genotypes were posteriorly grouped according to the subgroups obtained through GBS analysis. To minimize subsample redundancy, the core collection was designed using a set of 6540 SNPs distributed across all 16 chromosomes. Finally, a core collection comprising nine walnut genotypes (10% of the entire genotype set), including five cultivars, three seedling populations, and one landrace, was assembled. Genetic structure analysis indicated that the core collection has an uneven distribution in the landrace collection, which could be related to environmental conditions, and the genotypes of the landrace collection are similar. Overall, the results of this study and the establishment of the core collection will facilitate the improvement of walnut in future breeding programs.

The Origin of Spanish Durum Wheat and Olive Tree Landraces Based on Genetic Structure Analysis and Historical Records

In this study, the origin of Spanish durum wheat and olive tree landrace collections (preserved in seed banks and ex situ field collections, respectively) was traced throughout different studies on genetic structure analysis of different landrace collections and historical records of plant material circulation of these two crops in the south and east of Spain (the main cultivation areas). Although there were several groups of Spanish durum wheat landraces, they did not cluster geographically and likely came from intense grain circulation within the country and abroad (due to a high seeding rate of about 100 kg/ha and an unstable production) from the 15 to 19th centuries (especially the Maghreb and Sicily). However, Spanish olive tree landraces experienced lower genetic circulation over time and space due to the longevity of the species and the large size of the reproductive material. They can be clearly divided into two groups: those from the south, of Maghrebi origin, which most likely originated during the Islamic expansion of the 8–15th centuries, and those from the east, arriving most likely during Roman times from Italy. The genetic circularity levels of the plant material of these two crops are different.

New flavors from old wheats: exploring the aroma profiles and sensory attributes of local Mediterranean wheat landraces.

During the 20th century, the worldwide genetic diversity of wheat was sharply eroded by continual selection for high yields and industry demands for particular standardized qualities. A collection of Israeli and Palestinian landraces (IPLR) was established to represent genetic diversity, accumulated for ten millennia under diverse environments, which was mostly lost in this transition. As our long-term goal is to study this pre- Green Revolution genetic reservoir, herein we focus on its flour and bread quality and sensorial attributes. Initially, a database was built for the entire IPLR collection (n=901) holding both Triticum durum (durum wheat) and T. aestivum (bread wheat) which included genetic and phenotypic characterization of agronomic traits, grain and flour quality. Then, a representative subset of the IPLR was selected and compared to modern varieties for dough quality, rheology, aroma and taste using both whole and refined flours and breads. The sensory panel used 40 subjects who evaluated common protocol or sourdough breads made by four artisan bakers. Results show modern durum cultivar C-9 had superior rheological properties (gluten index, elasticity, dough development time) as compared with landraces, while bread landrace 'Diar Alla' was markedly preferable for baking in relation to the modern cultivar Gadish. Baking tests and subsequent sensory evaluation clearly demonstrated a preference toward refined breads, apart from whole breads prepared using sourdough starters. In bread wheat, loaves baked using landrace flour were scored higher in several quality parameters, whereas in durum lines, the opposite trend was evident. Loaves baked from landraces 'Diar Alla' and to a lesser extent 'Hittia Soada' presented a markedly different aroma from the control loaves prepared from modern flours, both in terms of overall compositions and individual compounds, including classes such as pyranones, pyrazines, furans and pyrroles (maltol). Modern lines, on the other hand, were consistently richer in terpenes and phenylpropanoids. Further analysis demonstrated a significant association between specific aroma classes and sensory attributes scored by panelists. The findings of the study may help advance new niches in the local wheat market aimed at health and nutrition including adapting durum varieties to the bread market and developing flavor-enhanced wholemeal breads.

Heritage genetics for adaptation to marginal soils in barley.

Future crops need to be sustainable in the face of climate change. Modern barley varieties have been bred for high productivity and quality; however, they have suffered considerable genetic erosion, losing crucial genetic diversity. This renders modern cultivars vulnerable to climate change and stressful environments. We highlight the potential to tailor crops to a specific environment by utilising diversity inherent in an adapted landrace population. Tapping into natural biodiversity, while incorporating information about local environmental and climatic conditions, allows targeting of key traits and genotypes, enabling crop production in marginal soils. We outline future directions for the utilisation of genetic resources maintained in landrace collections to support sustainable agriculture through germplasm development via the use of genomics technologies and big data.