Lamb's Quarters Research Articles

Exploring the anticandidal potential: Evaluating leaf extract efficacy and GC–MS metabolite profiling of Chenopodium album var. album L.

IntroductionChenopodium album var. album L., renowned as lamb's quarters, is a versatile medicinal plant, revealing its efficacy in treating various ailments. This study evaluated the in vitro antifungal activity of ethyl acetate extract of C. album var. album L. MethodsChenopodium album leaf extract demonstrated antifungal potential via the Agar well-diffusion and Broth-dilution method. Active fractions, obtained through column chromatography, were rigorously tested against Candida spp. The most potent fraction underwent detailed analysis with High-Performance Thin-Layer Chromatography (HPTLC), Gas Chromatography Mass Spectrometry (GC–MS), and Fourier-Transform Infrared Spectroscopy (FT-IR), for comprehensive characterization. ResultsThe ethyl acetate extract (EAE) exhibited significant antifungal activity, with Candida albicans being the most susceptible (inhibition zone of 14.67 ± 0.58a). Fractionation of the EAE yielded a highly active F2 fraction, which showed a remarkable zone of inhibition against C. albicans (17.50 ± 1.00a). Comprehensive examinations, including HPTLC, GC–MS, and FT-IR, identified 12 compounds in the F2 fraction, and the major compounds were Furo [2,3-c] pyridine, 2,3-dihydro-2,7-dimethyl, Bis (2-ethylhexyl) phthalate, and 2,3,4,4-Tretrapropyl-1-(trimethylsilyl)-1-(trimethylsilyloxy)-1,3-diaza-2,4-diborabutane. This study marks the first report of these 12 compounds identified from the F2 fraction of C. album. ConclusionThe present study highlights the antifungal activity of EAE and its fractions from C. album var. album that could be further explored for the selection of leads that may contribute to novel drug development.

Improvement of potato cultivation technology elements in production conditions

The results of improving potato cultivation technology by increasing the efficiency of plant protection system in the conditions of production experience are presented. The study was conducted in 2021–2023 on the lands of the OOO "Druzhba-2" (Bryansk region, Zhiryatinsky district). Two potato varieties of the first reproduction, Red Scarlett (Netherlands) and Gala (Germany), were the object of the study. The experiment scheme included two variants: 1) traditional potato protection system adopted in the farm (control); 2) improved protection system. Both protection systems are aimed at the prevention of pests (Colorado potato beetle, snap beetle larvae), weeds (cleavers, black nightshade, purple deadnettle, lamb's quarters, wild camomile, annual bluegrass, wind bent grass) and diseases (early blight, late blight, silver scurf, rhizoctoniose, various types of rots, pythium). Soils of the sites are sod-podzolic. The forecrop is winter rapeseed. The total area of the production experiment was 120 ha (60 ha under each variety). Potatoes were grown under intensive technology with conventional tillage system on a 75 × 28 cm pattern. Planting rate is 50 thousand tubers per 1 ha. The technology is designed to obtain tuber yields of 40–60 t/ha. Experimentally it has been established that the application of the improved technology with the use of a new plant protection system for the farm contributes to an increase in plant stand density and their safety at the time of harvesting by 2.5–3.0%, increase in the number of tubers from one plant by 7.5–10.0%, average weight of one tuber – by 7–11%, weight of tubers from one plant – by 14.5–23.0%, yield – by 17–26%, marketability – by 12.5–14.5%, marketable yield – by 32–45%, conditional net income – by 16.2–24.5%.

Perspective herbicides for post-sowing use in narrow-leafed lupin crops

Technical and economic efficiency of Pilot herbicide when applied on green leaf in narrow-leafed lupine crops was studied. The research was conducted in field and plot experiments according to the generally accepted methods in the southwestern part of the Non-Chernozem zone of Russia. The soil of the experimental field was gray forest light loamy in mechanical composition. The following application rates of the preparation were used: 1.5, 2.0 and 2.5 l/ha in the phase of appearance of two true leaves in lupine. The following plants showed high sensitivity even at the minimum application rate: cleavers (Galium aparine L.), wild buckwheat (Fallopia convolvulus L.), lamb's-quarters (Chenopodium album L.), one of the types of hemp nettle (Galeopsis sp. L.), shepherd's purse (Capsella bursa pastoris L. Medik), field violet (Viola arvensis Murr.), chickweed (Stellaria media L.). Application of GalactAlt graminicide (application rate 1.0 l/ha) after treatment of narrow-leafed lupine crops with Pilot herbicide promoted almost complete destruction of annual grass weeds. Application of Pilot herbicide had a favorable effect on nitrogen content in both dry aboveground mass and root mass of lupine. Reduction of weediness of crops when using Pilot contributed to the increase in lupine yield. In the variant with the application of 1.5 l/ha of the herbicide, the average yield of narrow-leafed lupine seeds exceeded the control by 0.53 t/ha in three years of the trials. The highest increase in seed yield of narrow-leafed lupine (0.96 t/ha) was observed in the variant that involved the application of herbicide Pilot at a concentration of 1.5 l/ha in the phase of one or two true leaves in lupine and herbicide GalactAlt at a concentration of 1.0 l/ha in the phase of lupine budding. Chemical analyses showed an increase in crude protein content in narrow-leafed lupine seeds by 2.3–4.2% when the crops were treated with Pilot herbicide compared to the control variant, where the treatment was not carried out.

Sporopollenin Capsules as Biomimetic Templates for the Synthesis of Hydroxyapatite and β-TCP.

Pollen grains, with their resilient sporopollenin exine and defined morphologies, have been explored as bio-templates for the synthesis of calcium phosphate minerals, particularly hydroxyapatite (HAp) and β-tricalcium phosphate (TCP). Various pollen morphologies from different plant species (black alder, dandelion, lamb's quarters, ragweed, and stargazer lily) were evaluated. Pollen grains underwent acid washing to remove allergenic material and facilitate subsequent calcification. Ragweed and lamb's quarter pollen grains were chosen as templates for calcium phosphate salts deposition due to their distinct morphologies. The calcification process yielded well-defined spherical hollow particles. The washing step, intended to reduce the protein content, did not significantly affect the final product; thus, justifying the removal of this low-yield step from the synthesis process. Characterisation techniques, including X-ray diffraction, scanning electron microscopy, Fourier-transform infrared spectroscopy, and thermal gravimetric analysis, confirmed the successful calcification of pollen-derived materials, revealing that calcified grains were principally composed of calcium deficient HAp. After calcination, biphasic calcium phosphate composed of HAp and TPC was obtained. This study demonstrated the feasibility of using pollen grains as green and sustainable bio-templates for synthesizing biomaterials with controlled morphology, showcasing their potential in biomedical applications such as drug delivery and bone regeneration.

Integrating GIS and irrigation water quality index approaches for identifying groundwater irrigation potential zones in Central Gujarat, India.

In this study, groundwater irrigation potential zones are identified by measuring an integrated irrigation water quality index using a GIS platform in the Vishwamitri River Basin (VRB) region, Gujarat, India. Classification of different irrigation potential zones is about the collective paraphernalia of thematic maps, namely, electrical conductivity (EC), sodium adsorption ratio (SAR), Na+, Cl-, and HCO3-, which principally regulate the groundwater quality for irrigation. The EC, SAR, and chloride values are > 3000 µS/cm, > 9meq/L, and > 10meq/L, respectively, indicating 98.78%, 41.78%, and 84.88% of the total area, respectively, belong to groundwater both hazardous and unsuitable for irrigation. Similarly, 39.55% of the total area is unsuitable for irrigation due to its high Na+ concentration. The permeability, porosity, and infiltration risk usually appear when excessive sodium ions reduce the rate of flow of irrigation water which moves in the lower soil zones. Similarly, high bicarbonate and chloride concentrations have been denoted to hamper the development of several crops causing symptoms analogous to lime-induced chlorosis due to toxic effects of the salt. The final irrigation potential map is marked by four levels, such as "most suitable," "moderately suitable," "moderately unsuitable," and "unsuitable for irrigation." Approximately, 56.69% of the total VRB region are covered with unsuitable for irrigation purposes. In such areas, irrigation must be avoided in usual conditions, and the halophytes, namely, Khati palak (Atriplex stocksii), Lamb's quarters (Chenopodium album), and Rudravanti (Cressa cretica) should be recommended with high soil permeability and proper drainage facilities. The soil must be highly permeable for groundwaters with high salinity and sodicity. Based on this study, policymakers and environmental managers can estimate the resource implications for drinking and irrigation.

Study of molecular mechanisms of herbicide binding t o target proteins of weeds by in silico methods

The aim of this study was to study of the molecular mechanisms of herbicide binding (pinoxaden, quizalofop- P-tephuryl, prometryn, metribuzin, metsulfuron- methyl) to target proteins (acetyl- CoA carboxylase, acetolactate synthase, cytochrome P450, p-hydroxyphenylpyruvate dioxygenase) of the weed species growing in Siberia: wild oat Avena fatua (L.), wheatgrass Elymus repens (L.), amaranth Amaranthus retro exus (L.), common lamb's quarters Chenopodium album (L.), bristlegrass Setaria viridis (L.). The work was carried out in the Novosibirsk region in 2022-2023. Using bioinformatic methods, virtual three- dimensional complexes of pesticides and their targets were created. A new approach has been proposed to identify the parameters of the cavity of binding sites, which consists in combining the alignment method and machine learning technology. Docking of pesticides with targets was carried out. The calculation of the binding energy indicates a high degree of stability of the ligand- protein complexes. The in silico approach we proposed may be useful for elucidating the behavior of herbicides when bound to weed enzymes. This approach allows a deeper understanding of the mechanism of action and ecotoxicological aspects of the use of pesticides. The studied complexes of herbicides and proteins have a potential high stability due to the low binding energy with receptors, which varies from -5.60 to -13.24 kcal/mol. The binding energy value did not show a direct dependence on the number of amino acid residues of the ligand- binding site. In general, the study supplemented some aspects of the molecular mechanisms of binding herbicides to target proteins of weeds, however, a more in-depth study is required in order to localize critical amino acid points involved in the biological functions of target proteins.

Genomic Sequence of Canadian Chenopodium berlandieri: A North American Wild Relative of Quinoa.

Chenopodium berlandieri (pitseed goosefoot) is a widespread native North American plant, which was cultivated and consumed by indigenous peoples prior to the arrival of European colonists. Chenopodium berlandieri is closely related to, and freely hybridizes with the domesticated South American food crop C. quinoa. As such it is a potential source of wild germplasm for breeding with C. quinoa, for improved quinoa production in North America. The C. berlandieri genome sequence could also be a useful source of information for improving quinoa adaptation. To this end, we first optimized barcode markers in two chloroplast genes, rbcL and matK. Together these markers can distinguish C. berlandieri from the morphologically similar Eurasian invasive C. album (lamb's quarters). Second, we performed whole genome sequencing and preliminary assembly of a C. berlandieri accession collected in Manitoba, Canada. Our assembly, while fragmented, is consistent with the expected allotetraploid structure containing diploid Chenopodium sub-genomes A and B. The genome of our accession is highly homozygous, with only one variant site per 3-4000 bases in non-repetitive sequences. This is consistent with predominant self-fertilization. As previously reported for the genome of a partly domesticated Mexican accession of C. berlandieri, our genome assembly is similar to that of C. quinoa. Somewhat unexpectedly, the genome of our accession had almost as many variant sites when compared to the Mexican C. berlandieri, as compared to C. quinoa. Despite the overall similarity of our genome sequence to that of C. quinoa, there are differences in genes known to be involved in the domestication or genetics of other food crops. In one example, our genome assembly appears to lack one functional copy of the SOS1 (salt overly sensitive 1) gene. SOS1 is involved in soil salinity tolerance, and by extension may be relevant to the adaptation of C. berlandieri to the wet climate of the Canadian region where it was collected. Our genome assembly will be a useful tool for the improved cultivation of quinoa in North America.

Phase sensitivity of some broad-leaved weed species to the herbicide Flex

The effect of herbicide Flex, AS (active ingredient fomesafen 250 g/l) on annual weed species common in soybean crops was studied. The studies were conducted under greenhouse conditions in 2019-2021. The sensitivity of ragweedAmbrosia artemisiifoliaL., Asian copper leafAcalypha australisL., common lamb's quartersChenopodium albumL., common dayflowerCommelina communisL., China juteAbutilon theophrastiMedik, trailing hollyhockHibiscus trionumL., St.-Paul’swortSigesbeckia pubescensMakino, green amaranthAmaranthus retroflexusL., elsholtziaElsholtzia pseudocristataLevl. et Vaniot, and Siberian cockleburXanthium sibiricumPatrin ex Widd was determined. Weed treatment with Flex at rates of 0.75, 1.0, 1.25 and 1.5 l/ha was carried out three times a season at different stages of plant growth and development. The level of sensitivity of weed species to herbicide was judged by the decrease in height and weight of the aboveground organs of the experimental plants compared to the control. It was found that Flex herbicide completely destroys plants of all tested species in the early stages of growth and development (1-4 true leaves). When treating weeds that have formed 3-10 leaves, ragweed, green amaranth, Asian copper leaf, trailing hollyhock and Siberian cocklebur remain highly sensitive to the drug action (reduction of the aboveground weight up to 94-100%). Application of Flex on overgrown plants leads to a significant decrease in its activity against all studied weed species. When used in the third term, the herbicide is effective only on the green amaranth, suppressing the mass of the plants by 76-86%.

Homegrown Foodways in West Virginia: Foraging and Relations with Jonathan Hall

Homegrown Foodways in West Virginia: Foraging and Relations with Jonathan Hall

Field evaluation of a deep learning-based smart variable-rate sprayer for targeted application of agrochemicals

The field performance of a newly developed novel smart variable-rate sprayer was evaluated. The sprayer uses convolutional neural networks (CNNs) for target detection and spot-applications of agrochemicals within potato (Solanum tuberosum L.) fields attacked by lamb's quarters (Chenopodium album L.) and corn spurry (Spergula arvensis L.) weeds and the early blight potato disease caused by Alternaria solani Sorauer. There was a non-significant effect of treatment conditions (i.e., cloudy, partly cloudy, and sunny) on spray volume during weed and diseased plant detection experiments (p-value = 0.93 and 0.75, respectively) showing that the smart sprayer performed well during all treatment conditions. There was a significant effect of spraying application techniques on the use of spray volume (p-value ≤ 0.05) reflecting a significant saving of spraying liquid during variable-rate application (VA). On average, the sprayer reduced spray volume by 47 and 51% for weeds and diseased plant detection experiments as compared to the values of chemicals applied at constant-rate application (CA), respectively, under all treatment conditions. The analysis of water-sensitive papers (WSP) data resulted in non-significant differences between CA and VA under all field conditions. These results suggest that this sprayer has a great potential to get a suitable spot application of agrochemicals and reduce the use of plant protection products thereby ensuring farm profits and environmental stewardship.

Herbicides for lamb's quarters control in soybean crops

The results of the study of toxicity level for the lamb's quarters (Chenopodium album L.) of well-known herbicides used in soybean crops as well as herbicides promising for soybean production in the Far East are presented. Lamb's quarters (Chenopodium album L.) is a highly noxious weed species that is widespread in the south of the region. Weediness monitoring conducted in 2006-2020 showed that this species was present in the Primorsky Territory on 63-100% of fields occupied by soybeans with an average growth density of 3-117 pcs/m2. In 2013-2020 in a series of experiments, conducted in the conditions of the vegetation house and on the experimental fields of the institute, the toxicity level for Common lamb's quarters was determined by 20 herbicides (based on 14 active substances) used in soybean crops. It was found that the control of this weed species is most effective when applying herbicides in the soil and treating plants with a height of up to 4-11 cm at the early stages of development - 1-3 pairs of leaves. Reliable suppression of lamb's quarters in experiments was provided by the soil (before sowing or before soybean seedlings) use of herbicides containing active substances metribuzin, imazetapir, trifluralin, pendimetalin and acetochlor. Among the active ingredients of the tested foliar herbicides, fomesafen, combinations of bentazone + acifluorfen and imazamox + chlorimuron-ethyl were the most toxic for lamb's quarters. It is recommended to use the following herbicidal preparations to control the spread of lamb's quarters: before sowing or sprouting of soybeans - Lazurit, Zenkor Ultra, Pivot, Proponit, Fabian; for processing vegetative plants - Flex, Galaksi Top, Concept, Classic Forte (Harmony Classic), Proponit.

Changes in leaf optical properties during tomato, common lamb's‐quarters, and redroot pigweed plant development

AbstractLeaf optical properties influence the red/far‐red light ratio (a signal of potential inter‐plant interaction) in plant canopies, which could alter inter‐plant interactions among species. Differences in leaf optical properties of species comprising a mixed population have significant implications for plant–plant interaction. Leaf optical properties at red (660 nm) and far‐red (730 nm) lights for the third true leaf of tomato (Solanum lycopersicon L.) and its two weeds common lamb's‐quarters (Chenopodium album L.) and redroot pigweed (Amaranthus retroflexus L.) were compared during plant development. The results showed that optical properties of the third true leaf at 660 and 730 nm changed with plant development in common lamb's‐quarters, redroot pigweed, and tomato, and the three species differed in this regard. Red/far‐red ratios of reflected (Rratio) and transmitted (Tratio) lights also changed with plant development in all species. Rratio and Tratio were greater in redroot pigweed compared to common lamb'squarters and tomato. These ratios significantly related with chlorophyll content and leaf mass per area. These differences, usually neglected, could influence growth and intra‐ and/or inter‐species interactions in plant communities comprising these species. These results provide valuable information for understanding eco‐physiology and function of vegetation cover and could help in development of effective weed management strategies.

Immunity Booster Activity of Lamb's Quarters: Nutritive Value and Economic Importance

Immunity Booster Activity of Lamb's Quarters: Nutritive Value and Economic Importance

Immunity Booster Activity of Lamb's Quarters: Nutritive Value and Economic Importance

Immunity Booster Activity of Lamb's Quarters: Nutritive Value and Economic Importance

Variability of morphophysiological parameters of Chenopodium album and Taraxacum officinale in the conditions of Kazan

One of the promising methods for the integral assessment of the quality of the environment and the vital activity of herbaceous plants is the assessment of the state of living organisms by the morphometric characters development stability. The study revealed an increase in the variability of morphological characters in the plants of lamb's quarters (Chenopodium album) and milk-witch gowan (Taraxacum officinalis) in areas with a high level of technogenic pollution. Phenotypes with smaller sizes of vegetative organs prevailed there, as compared with the area with a low level of pollution. At the same time, viable seeds and seedlings were formed in the plants of urban cenoses.

Investigation of the effects of non-living mulches and metribuzin on redroot pigweed and lamb'squarters in tomato

ABSTRACT-A field study was conducted to determine the influences of six mulch materials (organic and inorganic), including black plastic, transparent plastic, wheat straw, peat moss, coco peat, and sawdust and the metribuzin on the two weed species vegetative and reproductive growth cycles in a tomato field using a randomized complete block design with three replications at the Research Station of the Agricultural School, Shiraz University, Shiraz, Iran. According to the results of the analysis of variance, all evaluated traits of both weed species, and also tomato, were significantly influenced by treatments at p < 0.01.The longest and shortest time of redroot pigweed (Amaranthus retroflexus L.) emergence were recorded under the plastic mulch treatments at 28 days after transplanting (DAT) and organic mulches as well as metribuzin at 21 DAT, respectively. Plastic mulches deferred the time of lamb's quarters (Chenopodium album L.) emergence more than organic mulches up to 28.0%. Additionally, the longest time of lamb'squarters emergence was observed with coco peat at 29 DAT and the shortest time of its emergence was recorded under the application of clear plastic and sawdust mulches at 23.3 DAT. Results showed that, the highest marketable tomato yield (up to 19.2 kg m-2) was assessed in covered plots with black plastic mulch, while the lowest marketable yield was detected by 3.02 kg m-2 in the plots under the application of the sawdust treatment. In general, results demonstrated that the plastic mulches, particularly the black plastic sheet, could be reliable alternatives for metribuzin to control weed infestations in tomato fields.

Cercospora beticola: The intoxicating lifestyle of the leaf spot pathogen of sugar beet.

Cercospora leaf spot, caused by the fungal pathogen Cercospora beticola, is the most destructive foliar disease of sugar beet worldwide. This review discusses C. beticola genetics, genomics, and biology and summarizes our current understanding of the molecular interactions that occur between C. beticola and its sugar beet host. We highlight the known virulence arsenal of C. beticola as well as its ability to overcome currently used disease management strategies. Finally, we discuss future prospects for the study and management of C. beticola infections in the context of newly employed molecular tools to uncover additional information regarding the biology of this pathogen.Taxonomy Cercospora beticola Sacc.; Kingdom Fungi, Phylum Ascomycota, Class Dothideomycetes, Order Capnodiales, Family Mycosphaerellaceae, Genus Cercospora.Host rangeWell‐known pathogen of sugar beet (Beta vulgaris subsp. vulgaris) and most species of the Beta genus. Reported as pathogenic on other members of the Chenopodiaceae (e.g., lamb's quarters, spinach) as well as members of the Acanthaceae (e.g., bear's breeches), Apiaceae (e.g., Apium), Asteraceae (e.g., chrysanthemum, lettuce, safflower), Brassicaceae (e.g., wild mustard), Malvaceae (e.g., Malva), Plumbaginaceae (e.g., Limonium), and Polygonaceae (e.g., broad‐leaved dock) families.Disease symptomsLeaves infected with C. beticola exhibit circular lesions that are coloured tan to grey in the centre and are often delimited by tan‐brown to reddish‐purple rings. As disease progresses, spots can coalesce to form larger necrotic areas, causing severely infected leaves to wither and die. At the centre of these spots are black spore‐bearing structures (pseudostromata). Older leaves often show symptoms first and younger leaves become infected as the disease progresses.ManagementApplication of a mixture of fungicides with different modes of action is currently performed although elevated resistance has been documented in most employed fungicide classes. Breeding for high‐yielding cultivars with improved host resistance is an ongoing effort and prudent cultural practices, such as crop rotation, weed host management, and cultivation to reduce infested residue levels, are widely used to manage disease.Useful website https://www.ncbi.nlm.nih.gov/genome/11237?genome_assembly_id=352037

USING EXTRACTS OF LAMB'S QUARTERS WEED FOR CONTROL OF Alternaria alternata

Antifungal potential of extracts of different parts of lamb's quarters weed (Chenopodium album L.) against a plant pathogenic fungus Alternaria alternate assessed through laboratory bioassays. Different parts of the weed were soaked in methanol for two weeks to get the extracts. The solvent was evaporated under reduced pressure, and different concentrations of the extracts of each plant part (1 to 5%) were prepared in malt extract broth. Leaf, root and fruit extracts exhibited pronounced antifungal activity resulting in 23–95%, 29–96% and 9–94% suppression in biomass of A. alternata. The effect of stem extract was insignificant. This study concludes that all parts of C. album except stem contain potent antifungal constituents to control A. alternata.

Seed Germination and Seedling Vigor Improvement by Chenopodium(common lambs quarters) leaf extract and ChenopodiumSalt Bladders in Rice (Oryzasativa L.)

Chenopodium is a halophyte which absorbs salt from the soil and encrusts inaerial parts particularly in leaves which is known as chenopod salt bladders.Therefore, an experiment was conducted to enhance the seed quality in riceby treating with these bladders. Results showed that significant improvementin seed germination and seedling vigor was recorded in rice. The seedssoaked in Chenopodium leaf extract along with salt bladders @ 0.5 % orsalt bladders alone @ 0.6 % for 16 h at 1:1 (w/v) ratio recorded the highestgermination and seedling vigor. It is analyzed that the Chenopodium leafextract and its salt bladders contains more amount of minerals particularlyphosphorous (0.50%, 0.15%), potassium (0.83%, 1.11%) nitrogen (2.52%,2.21%), calcium (16.00 ppm, 22.40 ppm), magnesium (190.56 ppm,193.40 ppm), sodium (4.14 mg 100 g-1, 6.57 mg 100 g-1), chloride (0.14mol.L-1, 0.17 mol.L-1), total phenol (5.00 mg g-1, 16.12 mg g-1 ) and poly phenoloxidase (0.20 ΔA g-1min-1, 0.10 ΔA g-1min-1), respectively which favoured theenhancement of seed quality in rice.

An automatic visible-range video weed detection, segmentation and classification prototype in potato field

Weeds might be defined as destructive plants that grow and compete with agricultural crops in order to achieve water and nutrients. Uniform spray of herbicides is nowadays a common cause in crops poisoning, environment pollution and high cost of herbicide consumption. Site-specific spraying is a possible solution for the problems that occur with uniform spray in fields. For this reason, a machine vision prototype is proposed in this study based on video processing and meta-heuristic classifiers for online identification and classification of Marfona potato plant (Solanum tuberosum) and 4299 samples from five weed plant varieties: Malva neglecta (mallow), Portulaca oleracea (purslane), Chenopodium album L (lamb's quarters), Secale cereale L (rye) and Xanthium strumarium (coklebur). In order to properly train the machine vision system, various videos taken from two Marfona potato fields within a surface of six hectares are used. After extraction of texture features based on the gray level co-occurrence matrix (GLCM), color features, spectral descriptors of texture, moment invariants and shape features, six effective discriminant features were selected: the standard deviation of saturation (S) component in HSV color space, difference of first and seventh moment invariants, mean value of hue component (H) in HSI color space, area to length ratio, average blue-difference chrominance (Cb) component in YCbCr color space and standard deviation of in-phase (I) component in YIQ color space. Classification results show a high accuracy of 98% correct classification rate (CCR) over the test set, being able to properly identify potato plant from previously mentioned five different weed varieties. Finally, the machine vision prototype was tested in field under real conditions and was able to properly detect, segment and classify weed from potato plant at a speed of up to 0.15 m/s.