Lake In Central Italy Research Articles

Mapping the occurrence of Eustrongylides spp. in fish species caught from six lakes in Central Italy (Tuscany and Latium regions): Implications for local fishery supply chains

In the present study, in order to expand the knowledge on the distribution of parasites of the genus Eustrongylides in never investigated Italian lacustrine areas of Tuscany and Latium, fish specimens from four Tuscan lakes (Bilancino, Chiusi, Montedoglio and San Cipriano) and two Latium lakes (Bolsena and Bracciano) were collected and analyzed. The parasitological analysis, consisting of a visual inspection followed by a chloro-peptic digestion, was performed on 1650 specimens belonging to 17 species: European perch (Perca fluviatilis), Pumpkinseed (Lepomis gibbosus), Pike-perch (Sander lucioperca), Largemouth black bass (Micropterus salmoides), Pike (Esox lucius), Big-scale sand smelt (Atherina boyeri), Tench (Tinca), European Whitefish (Coregonus lavaretus), Channel catfish (Ictalurus punctatus), Chub (Squalius cephalus), Rudd (Scardinius erythrophthalmus), Common bleak (Alburnus alborella), South European roach (Sarmarutilus rubilio), South European nase (Protochondrostoma genei), Italian riffle dace (Telestes muticellus), Goldfish (Carassius auratus), and Freshwater bream (Abramis brama). Eustrongylides spp. larvae were only found in one specimen of European perch caught in Bracciano Lake and purchased from a local fishmonger (P = 3.3%; MI = 1; MA = 0.03). The only isolated Eustrongylides spp. larva was submitted to molecular analysis of the ITS gene region and identified as E. excisus. This finding adds Bracciano Lake to the list of the several Italian lakes in which nematodes belonging to this genus have been reported. Even if the observed prevalence is currently low, the presence of the parasite in this local freshwater fish supply chain requires FBOs to adopt risk management measures to prevent contaminated products from reaching final consumer. Moreover, future investigations will show if the parasite expands in these areas.

Climate-driven growth dynamics and trend reversal of Fagus sylvatica L. and Quercus cerris L. in a low-elevation beech forest in Central Italy

In highly climate-change-sensitive regions, such as the Mediterranean, increasing knowledge of climate-driven growth dynamics is required for habitat conservation and forecasting species adaptability under future climate change. In this study, we test a high spectrum of climatic signals, not only monthly and seasonal but also on a multi-year scale and include the single tree analysis to answer this issue, focusing on a low-elevation thermophilic old-growth beech forest surrounding the Bracciano Lake in Central Italy. Through a dendroecological and isotope analysis, we evaluate both short- and long-term sensitivity of F. sylvatica and the coexisting better-drought-adapted species Q. cerris to climatic and hydrological variability in terms of growth reduction and δ13C responses. After the 1990s, beech trees showed a climate-driven decrease in growth compared to oak, especially after 2003 (−20 % of basal area increment), with a significant growth trend reversal between the species. For F. sylvatica, the significant correlations with precipitation decreased, whereas for Q. cerris, they increased, with a higher number of trees positively influenced. However, the temperature highlighted more clearly the contrasting climate–growth correlation pattern between the two species. In F. sylvatica after the ‘90s, the negative effect of temperatures has significantly intensified, as shown by past summer values up to four years previously, involving about half of the trees. Surprisingly, the water-level fluctuations showed a highly significant influence on tree-ring growth in both species. Nevertheless, it reduced after the ‘90s. Finally, Q. cerris trees showed a significantly higher ability to recover their growth levels after extreme droughts (+55 %). The growth trend reversal and the shift in iWUE of the last years may point to potential changes in the future species composition, raising the need for climate-adaptive silviculture (e.g., selective thinning) to reduce growth decline, enhance resilience and favour the natural regeneration of the target species for habitat conservation.

Detection of Morganella morganii bound to a plastic substrate in surface water

ObjectivesAround the globe, escalation in rare opportunistic microbial infections is alarming as they are heading steadily towards 'superbug' status. In aquatic ecosystems, plastic fosters multidrug-resistant pathogenic bacteria and plays a significant role in trafficking antibiotic-resistant genes. In this study, we focused on a multidrug-resistant bacterial strain isolated from microbial communities found on plastic substrates of a volcanic lake in central Italy. MethodsExtended-spectrum beta-lactamase-producing strains were isolated from both raw water and plastic substrates for a comparative investigation using microbiological and molecular methods, and antibiotic susceptibility profiling was performed against a panel of ten antibiotics. ResultsMolecular identification and Basic Local Alignment Search Tool analysis confirmed an almost identical sequencing pattern of two isolated strains and their homology with Morganella morganii. Antibiotic susceptibility tests revealed their resistance to almost all tested antibiotics. Class 1 integron-associated gene (intI1) and seven antibiotic resistance genes were detected in both strains, confirming their superbug status. ConclusionTo our knowledge, this is the first study on the characterization of extended-spectrum beta-lactamase-producing M. morganii isolated from the biofilm of plastic substrates, depicting the potential toxicity of plastic in harbouring and dispersing virulent, multidrug-resistant, opportunistic human pathogens.

Assessment of PRISMA water reflectance using autonomous hyperspectral radiometry

Hyperspectral remote sensing reflectance (Rrs) derived from PRISMA in the visible and infrared range was evaluated for two inland and coastal water sites using above-water in situ reflectance measurements from autonomous hyper- and multispectral radiometer systems. We compared the Level 2D (L2D) surface reflectance, a standard product distributed by the Italian Space Agency (ASI), as well as outputs from ACOLITE/DSF, now adapted for processing of PRISMA imagery. Near-coincident Sentinel-3 OLCI (S3/OLCI) observations were also compared as it is a frequent data source for inland and coastal water remote sensing applications, with a strong calibration and validation record. In situ measurements from two optically diverse sites in Italy, equipped with fixed autonomous hyperspectral radiometer systems, were used: the REmote Sensing for Trasimeno lake Observatory (RESTO), positioned in a shallow and turbid lake in Central Italy, and the Acqua Alta Oceanographic Tower (AAOT), located 15 km offshore from the lagoon of Venice in the Adriatic Sea, which is characterised by clear to moderately turbid waters. 20 PRISMA images were available for the match-up analysis across both sites. Good performance of L2D was found for RESTO, with the lowest relative (Mean Absolute Percentage Difference, MAPD < 25%) and absolute errors (Bias < 0.002) in the bands between 500 and 680 nm, with similar performance for ACOLITE. The lowest median and interquartile ranges of spectral angle (SA < 8°) denoted a more similar shape to the RESTO in situ data, indicating pigment absorption retrievals should be possible. ACOLITE showed better statistical performance at AAOT compared to L2D, providing R2 > 0.5, Bias < 0.0015 and MAPD < 35%, in the range between 470 and 580 nm, i.e. in the spectral range with highest reflectances. The addition of a SWIR based sun-glint correction to the default atmospheric correction implemented in ACOLITE further improved performance at AAOT, with lower uncertainties and closer spectral similarity to the in situ measurements, suggesting that ACOLITE with glint correction was able to best reproduce the spectral shape of in situ data at AAOT. We found good results for PRISMA Rrs retrieval in our study sites, and hence demonstrated the use of PRISMA for aquatic ecosystem mapping. Further studies are needed to analyse performance in other water bodies, over a wider range of optical properties.

Microplastics and Brominated Flame Retardants in Freshwater Fishes From Italian Lakes: Implication for Human Health

Microplastics (MPs) and brominated flame retardants (BFRs) represent a synergic threat for aquatic environments and organisms' health status, with an additional concern over food quality and food security for species of commercial interest. In this study, the ingestion of MPs, levels of polybrominated diphenyl ethers (PBDEs), and hexabromocyclododecane (HBCDs) were assessed in organisms from two lakes of Central Italy, with the aims of exploring the bioavailability of these pollutants in freshwater environments, the possible translocation of MPs from digestive to edible tissues, and the relationship between MPs ingestion and bioaccumulation of BFRs. The fish Perca fluviatilis, Anguilla anguilla, Carassus auratus, and the crayfish Procambarus clarkii, all species commercialized for human consumption, were caught in Trasimeno Lake; moreover, P. fluviatilis and Rutilus rutilus were also sampled in Piediluco, a lake strongly influenced by industrial and anthropogenic activities, where fishing for commercial purpose is forbidden. With the exception of C. auratus which showed the highest frequency of MPs ingestion (75%), species from Piediluco Lake exhibited a more elevated percentage of organisms positive to MPs ingestion (45%) and higher levels of PBDEs and HBCDs (mean values of 343 and 792 pg/g, respectively, in P. fluviatilis; 445 and 677 pg/g, respectively, in R. rutilus) than Trasimeno species (25% frequency of MPs ingestion, mean values between 6 and 163 pg/g for PBDEs and 5-107 pg/g for HBCDs). Polyester fibers dominated among MP typologies, and a high occurrence of man-made natural fibers was recorded. The number of MPs extracted in the gastrointestinal tracts of fish and soft bodies of crayfishes positive to MPs ingestion ranged between 1 and 2, whereas no MPs were found in fish filets. Given these results, the risk related to human consumption of Trasimeno organisms appears very low, whereas further investigations are required to better elucidate the possible role of MPs pollution in modulating chemical bioaccumulation in edible tissues. This study contributed to assess both environmental quality and food safety, reinforcing the use of bioindicator species for monitoring plans, in accordance with European recommendations.

The importance of being petioled: leaf traits and resource-use strategies in Nuphar lutea

Intraspecific trait variability (ITV) can be considerably high and reveal plant local adaptation. The aim of this study is to investigate ITV of leaf traits (including petioles) in a rooted floating-leaved macrophyte (Nuphar lutea) at the local scale. We expected to see changes in resource-use strategies in relation to water and sediment properties. 96 leaves were sampled in a hyper-eutrophic shallow lake in central Italy, together with environmental parameters. Results highlight the influence of water depth and sediments in modulating environmental conditions and thus leaf traits. Leaf area and fresh and dry weight increased with water depth, a relation that catches the construction costs of petioles. The negative relation found between specific petiole area and conductivity suggests a stress imposed by high nutrient availability which triggers unfavorable conditions for N. lutea. We demonstrated the relevance of petiole traits for a rooted macrophyte. Petiole aerenchyma or photosynthetic extra surfaces fundamentally contribute to fit the environment, opening new questions for the functional investigation of macrophytes. Excluding petioles in the assessment of leaf traits reduces the possibility to understand the ecological/adaptive processes of nympheids. New data are urgently needed to increase the number of data and species to be analyzed.

Ianula (1970) revisited. Lessons from a pioneering study

More than fifty years ago, G.E. Hutchinson published “Ianula: an account of the history and development of the Lago di Monterosi, Latium, Italy”, a detailed report of one of the first multi-disciplinary palaeolimnological studies. The main result of that study was that the last Glacial climate in peninsular Italy was not only cold but also dry, in contrast with the assumption prevalent at that time of wet "pluvials" in Glacial stages of the northern Mediterranean. These finding were confirmed by more recent studies on other volcanic lakes in Italy. Furthermore, the authors found a major change in ecosystem structure and concluded that it was caused by the building of a road in Roman time. Comparing Monterosi pollen profile with those obtained from cores in other lakes in Central Italy and in the Adriatic Sea, I suggest an alternative hypothesis linking the dramatic environmental change recorded to soil development during forest onset at the beginning of the Holocene. The original report made available a large share of the data set, as a pioneering example of Open Data, allowing a re-examination of the results and the formulation of new hypotheses, underlying the importance of open data in environmental science.

Inter-Comparison of Methods for Chlorophyll-a Retrieval: Sentinel-2 Time-Series Analysis in Italian Lakes

Different methods are available for retrieving chlorophyll-a (Chl-a) in inland waters from optical imagery, but there is still a need for an inter-comparison among the products. Such analysis can provide insights into the method selection, integration of products, and algorithm development. This work aims at inter-comparison and consistency analyses among the Chl-a products derived from publicly available methods consisting of Case-2 Regional/Coast Colour (C2RCC), Water Color Simulator (WASI), and OC3 (3-band Ocean Color algorithm). C2RCC and WASI are physics-based processors enabling the retrieval of not only Chl-a but also total suspended matter (TSM) and colored dissolved organic matter (CDOM), whereas OC3 is a broadly used semi-empirical approach for Chl-a estimation. To pursue the inter-comparison analysis, we demonstrate the application of Sentinel-2 imagery in the context of multitemporal retrieval of constituents in some Italian lakes. The analysis is performed for different bio-optical conditions including subalpine lakes in Northern Italy (Garda, Idro, and Ledro) and a turbid lake in Central Italy (Lake Trasimeno). The Chl-a retrievals are assessed versus in situ matchups that indicate the better performance of WASI. Moreover, relative consistency analyses are performed among the products (Chl-a, TSM, and CDOM) derived from different methods. In the subalpine lakes, the results indicate a high consistency between C2RCC and WASI when aCDOM(440) &lt; 0.5 m−1, whereas the retrieval of constituents, particularly Chl-a, is problematic based on C2RCC for high-CDOM cases. In the turbid Lake Trasimeno, the extreme neural network of C2RCC provided more consistent products with WASI than the normal network. OC3 overestimates the Chl-a concentration. The flexibility of WASI in the parametrization of inversion allows for the adaptation of the method for different optical conditions. The implementation of WASI requires more experience, and processing is time demanding for large lakes. This study elaborates on the pros and cons of each method, providing guidelines and criteria on their use.

Plastic abundance and seasonal variation on the shorelines of three volcanic lakes in Central Italy: can amphipods help detect contamination?

Despite the exponential increase of studies on plastic debris in recent years, there are still few works focusing on the problem as it relates to inland waters: little is known about the accumulation and dispersion dynamics on lake shores, and there are no standardized sampling methods for monitoring purposes. The accumulation of plastic litter in natural habitats also threatens the resident organisms. In this paper, we investigated the abundance and accumulation of plastic particles, ranging in size from 1 to 50mm, from the beach sediment of three volcanic lakes in Central Italy: Albano, Bracciano, and Vico. The collection was designed to define the most important variables that one must consider in order to obtain a representative sample of plastic litter in a lake environment. In view of the high heterogeneity of sampling protocols used, comparison among the obtained results is limited and sometimes impossible. By using one of the proposed sampling methodologies, and critically analyzing the results, we aimed to highlight a possible monitoring criterion and to identify specific elements that can be meaningful and representative. The samples were collected in May and September 2017. For each lake, we sampled plastic items and sediments from two beaches. Albano contained the largest amount of plastic (in weight), while Bracciano had the largest number of particles. Our observations lead us to infer that the number of particles is the parameter most sensitive to environmental variations, as well as the more suitable for monitoring with greater definition the differences between sites. Moreover, sampling should be taken in different seasons, following a sampling pattern that includes at least two beaches placed in strategic positions with respect to wind and waves.In order to identify new indicators to evaluate the entry points of plastic into the food web, we collected, from the same sites analyzed, some specimens of the Talitrid Amphipod Cryptorchestia garbinii, a detritivorous species having a critical role in debris turnover of these environments. To investigate the microplastic (MP) ingestion in natural conditions, we analyzed their digestive tracts with both Nile red staining method and micro-FTIR spectroscopy. The analyses confirmed that C. garbinii was able to ingest plastics in natural conditions. Therefore, it can signify one of the entry points for microplastics (MPs) in the trophic chain. This observation constitutes the first evidence of MP ingestion in this species.

Behaviour and eating habits as determinants for human opisthorchiasis in the Bolsena Lake area, Italy.

In Italy, food-borne outbreaks of opisthorchiasis have occurred since 2003, all of them originating from some lakes in Central Italy where the only European liver fluke, Opisthorchis felineus (Rivolta, 1884) is endemic. The aim of the present study is to investigate the factors that may facilitate the life cycle of O. felineus in the area of Bolsena Lake, the knowledge of the local population about opisthorchiasis, and preventative healthcare measures. We performed a descriptive observational study through the submission of questionnaires to three target populations: fishermen who fish exclusively in Bolsena Lake, restaurateurs whose businesses are close to the lake and general population living in the province of Viterbo. Results showed hazardous behaviours and eating habits in the three target populations, as well as a poor knowledge of opisthorchiasis and preventative healthcare measures.

Water Level Measurements from Drones: A Pilot Case Study at a Dam Site

Unmanned Aerial Vehicles (UAVs) are now filling in the gaps between spaceborne and ground-based observations and enhancing the spatial resolution and temporal coverage of data acquisition. In the realm of hydrological observations, UAVs play a key role in quantitatively characterizing the surface flow, allowing for remotely accessing the water body of interest. In this paper, we propose a technology that uses a sensing platform encompassing a drone and a camera to determine the water level. The images acquired by means of the sensing platform are then analyzed using the Canny method to detect the edges of water level and of Ground Control Points (GCPs) used as reference points. The water level is then retrieved from images and compared to a benchmark value obtained by a traditional device. The method is tested at four locations in an artificial lake in central Italy. Results are encouraging, as the overall mean error between estimated and true water level values is around 0.05 m. This technology is well suited to improve hydraulic modeling and thus provides reliable support to flood mitigation strategies.

Aquatic vegetation in deep lakes: Macrophyte co-occurrence patterns and environmental determinants

Our aims were to test the hypothesis that in deep lakes the co-occurrence patterns of macrophytes are not random, and to compare the relative contribution of the main environmental determinants (light, water and sediment parameters, phytoplankton) in structuring aquatic vegetation. We collected data from five deep Chara-dominated lakes in Central Italy along gradients of depth (33 to 165 m), dimension (1.7 to 114.5 km2) and water trophic conditions (12.4 to 41.3 μg L-1 of total phosphorous). Twenty-five sampling plots per lake were randomly selected at five predetermined depths (1.5, 3.0, 6.0, 12.0 and 20.0 m; n=5) within homogenous littoral sectors. Data were explored by a null model analysis using the checkerboard score (C-score) index, and Canonical Correspondence Analysis. Our data verify the not random co-occurrence patterns of macrophyte’ communities in deep lakes. However, present data suggested that C-scores are strictly dependent on lake’ trophic status: low nutrient loads, in both water and sediments, seemed to be reflected in a not random co-occurrence zonation of macrophytes. Summarizing, it is fundamental evaluate the local effects of lake trophy on the macrophyte community dynamics both in time and space before inquiring about mutual links. If it fails to assess macrophyte co-occurrence patterns, it may be not possible to identify the determinants of the spatial arrangement of macrophytes and, in turn, the conservation status or the ongoing dynamics of lakes.

Integrated Water Resources Management in a Lake System: A Case Study in Central Italy

Lake Trasimeno is a closed lake in Central Italy and in historically its water level has been affected by wide fluctuations mostly depending on the climate. The lake has suffered many water crises due to water scarcity and in recent decades, droughts have also severely affected the economic and environmental situation. The aim of this study was to analyze the possibility of limiting these severe level fluctuations by evaluating of feasible water resource management policies that could also reduce the environmental stress of this area. Therefore, a specific decision support system (DSS) has been developed in order to simulate different scenarios for the entire water system of the Trasimeno area. In particular, the hydrological model implemented in the DSS allowed for the simulation and validation of different management policy hypotheses for the water resource in order to mitigate environmental and water crises for the Lake Trasimeno. Results indicated that it is possible to transfer a certain amount of water from nearby reservoirs without affecting the availability of the resource for specific users. In this way, Lake Trasimeno can benefit both from an increase in water levels in the lake, so a possible better situation in quantitatively and qualitatively.

Microplastic pollution in lakes and lake shoreline sediments – A case study on Lake Bolsena and Lake Chiusi (central Italy)

Rivers and effluents have been identified as major pathways for microplastics of terrestrial sources. Moreover, lakes of different dimensions and even in remote locations contain microplastics in striking abundances. This study investigates concentrations of microplastic particles at two lakes in central Italy (Lake Bolsena, Lake Chiusi). A total number of six Manta Trawls have been carried out, two of them one day after heavy winds occurred on Lake Bolsena showing effects on particle distribution of fragments and fibers of varying size categories. Additionally, 36 sediment samples from lakeshores were analyzed for microplastic content. In the surface waters 2.68 to 3.36 particles/m3 (Lake Chiusi) and 0.82 to 4.42 particles/m3 (Lake Bolsena) were detected, respectively. Main differences between the lakes are attributed to lake characteristics such as surface and catchment area, depth and the presence of local wind patterns and tide range at Lake Bolsena. An event of heavy winds and moderate rainfall prior to one sampling led to an increase of concentrations at Lake Bolsena which is most probable related to lateral land-based and sewage effluent inputs. The abundances of microplastic particles in sediments vary from mean values of 112 (Lake Bolsena) to 234 particles/kg dry weight (Lake Chiusi). Lake Chiusi results reveal elevated fiber concentrations compared to those of Lake Bolsena what might be a result of higher organic content and a shift in grain size distribution towards the silt and clay fraction at the shallow and highly eutrophic Lake Chiusi. The distribution of particles along different beach levels revealed no significant differences.

Cyanobacteria biennal dynamic in a volcanic mesotrophic lake in central Italy: Strategies to prevent dangerous human exposures to cyanotoxins

Vico Lake, a volcanic meso-eutrophic lake in Central Italy, whose water is used for drinking and recreational activities, experienced the presence of the microcystins (MC) producing cyanobacterium Planktothrix rubescens. In order to assess the human health risks and to provide the local health authorities with a scientific basis for planning tailored monitoring activities, we studied P. rubescens ecology and toxicity for two years. P. rubescens generally dominated the phytoplankton community, alternating with Limnothrix redekei, potentially toxic. P. rubescens was distributed throughout the water column during winter; in summer it produced intense blooms where drinking water is collected (−20 m); here MC were detected all year round (0.5–5 μg/L), with implications for drinking water quality. In surface waters, MC posed no risk for recreational activities in summer, while in winter surface blooms and foams (containing up to 56 μg MC/L) can represent a risk for people and children practicing water sports and for animals consuming raw water.Total phosphorus, phosphate and inorganic nitrogen were not relevant to predict densities nor toxicity; however, a strong correlation between P. rubescens density and aminopeptidase ectoenzymatic activity, an enzyme involved in protein degradation, suggested a role of organic nitrogen for this species. The fraction of potentially toxic population, determined both as mcyB+/16SrDNA (10–100%) and as the MC/mcyB+ cells (0.03–0.79 pg MC/cell), was much more variable than usually observed for P. rubescens. Differently from other Italian and European lakes, the correlation between cell density or the mcyB+ cells and MC explained only ∼50 and 30% of MC variability, respectively: for Vico Lake, monitoring only cell or the mcyB+ cell density is not sufficient to predict MC concentrations, and consequently to protect population health. Finally, during a winter bloom one site has been sampled weekly, showing that monthly sampling during such a phase could greatly underestimate the ‘hazard’. Our results highlight the need to adopt a stepwise monitoring activity, considering the lake and the cyanobacteria specific features. This activity should be complemented with communication to the public and involvement of stakeholders.

Occurrence of non-steroidal anti-inflammatory drugs in surface waters of Central Italy by liquid chromatography–tandem mass spectrometry

Non-steroidal anti-inflammatory drugs (NSAIDs) are considered to be the cure-all of any pain: from headache and cold symptoms to toothaches and even labour pain. Their wide use in human medicine has been favoured by the possibility to purchase them without medical prescription, their low cost and by the absence of opioid-like side effects. At the same time, their administration to domestic and food-producing animals has become a common practice to improve their well-being. Therefore, human and veterinary applications are the main sources of NSAIDs in the environment and the major pathways are excretion and discharge through sewage treatment plants. Although their environmental occurrence is well-known, there is a lack of data regarding their levels in surface waters of Central Italy. In this study, a monitoring campaign was arranged in some of the most important rivers and lakes of Central Italy, characterised by a different anthropic impact, in spring–summer and autumn–winter 2012. A broad range of NSAIDs for human and animal use was analysed through a reliable analytical method based on liquid chromatography–tandem mass spectrometry, appropriately developed and validated. Results have shown the constant presence of all drugs commonly used in human medicine with a composition that mirrored the incidence of seasonal diseases quite well. A veterinary drug (flunixin) was found in Bracciano Lake (Rome district), an important tourist attraction surrounded by farmlands. Salicylic acid is a phytohormone and this explains its presence in all the analyzed samples. All the results collected during the extensive survey have proved that Central Italy is aligned with the rest of Europe since its natural waters have shown low levels of contamination (ng L−1) but with a chronic input.

CORINE land cover and floristic variation in a Mediterranean wetland

The aims of the present study were to: (1) investigate whether CORINE land cover classes reflect significant differences in floristic composition, using a very detailed CORINE land cover map (scale 1:5000); (2) decompose the relationships between floristic assemblages and three groups of explanatory variables (CORINE land cover classes, environmental characteristics and spatial structure) into unique and interactive components. Stratified sampling was used to select a set of 100-m(2) plots in each land cover class identified in the semi-natural wetland surrounding a lake in central Italy. The following six classes were considered: stable meadows, deciduous oak dominated woods, hygrophilous broadleaf dominated woods, heaths and shrublands, inland swamps, canals or watercourses. The relationship between land cover classes and floristic composition was tested using several statistical techniques in order to determine whether the results remained consistent with different procedures. The variation partitioning approach was applied to identify the relative importance of three groups of explanatory variables in relation to floristic variation. The most important predictor was land cover, which explained 20.7% of the variation in plant distribution, although the hypothesis that each land cover class could be associated with a particular floristic pattern was not verified. Multi Response Permutation Analysis did not indicate a strong floristic separability between land cover classes and only 9.5% of species showed a significant indicator value for a specific land cover class. We suggest that land cover classes linked with hygrophilous and herbaceous communities in a wetland may have floristic patterns that vary with fine scale and are not compatible with a land cover map.

Short note on an alien Planorbella (Gastropoda: Pulmonata) in volcanic lakes in Central Italy

This brief note provides further data on the presence, abundance, and shell morphology of a snail species belonging to the genus Planorbella, found in central Italy in the crater lakes Albano and Nemi in 1986 and 2001, respectively. The snail is an alien species in Italy and is very interesting from a taxonomic point of view. A short history of its discovery in the two lakes is also provided.

Application of Remote Sensing in Water Resource Management: The Case Study of Lake Trasimeno, Italy

Satellite multi-sensor data were used to investigate the evolution in time and space of Lake Trasimeno, a shallow and turbid lake in central Italy. Large-swath MERIS and MODIS sensors were proposed for regular broad scale monitoring of water quality, having compared the retrieved chlorophyll-a (Chl-a) concentration, Secchi disk (SD) depth and surface water temperature with the 2005–2008 time-series of the in situ data. Although, in a shorter time span, also the MERIS-derived total suspended matter (TSM) matched the in situ data. MERIS-derived water quality products confirmed the meso-eutrophic conditions of Lake Trasimeno (average Chl-a = 8.5 mg/m3) and the low levels of transparency (average SD = 1 m). A negative correlation found between water levels and Chl-a suggest the importance of maintaining water levels as close as possible to the hydrometric zero. A spatial analysis of TSM also reveals how small tributaries may affect the load of suspended solids in the southern part of the lake. Higher spatial resolution satellite images were exploited both to describe land use/cover transformation from 1978 to 2008 and to assess the recent changes in macrophyte colonisation patterns. Land cover change detection analysis results showed a decrease in cultivated areas starting from the early Nineties and the subsequent increase in unproductive terrain (bare land and pastures) and natural woods as well as the changing fragmentation of agricultural areas through time. A reduction in macrophyte beds from 2003 to 2008 was also observed. We expect the results of this study to support local water authorities in redrawing the management plan of Lake Trasimeno.

Association of riparian features and water chemistry with reed litter breakdown in a volcanic lake (Lake Vico, Italy)

To assess riparian influences on lake functioning and develop simple tools for lake health monitoring, the association of remotely-sensed riparian factors and water chemistry with the breakdown rate of reed litter was examined in Lake Vico, a volcanic lake in central Italy. The decomposition rate of Phragmites australis was determined as leaf mass loss from litterbags at 6 locations during summer 2000. Water temperature, dissolved oxygen, nitrogen and phosphorus concentrations, pH, conductivity, percent organic matter in sediments and effective fetch were also assessed at each site. Riparian factors (i.e. shoreline complexity, riparian vegetation cover, and distance from shoreline) were estimated from satellite images (Landsat VII). External organic loadings largely explained spatial heterogeneity in lake metabolism. Decomposition was fastest in the northern area of the lake that drained most of the watershed, including a larger portion in wetland area. Rates correlated exponentially with soluble reactive phosphorus (SRP) concentration, and were positively associated with total-P (TP) concentration and organic matter percentage in sediments, which, in turn, were influenced by the riparian-related factors and waveexposure (fetch). A relationship existed between the index of riparian load, RL, which combined the three riparian-related factors, and both decomposition rate and SRP, suggesting that the riparian index is a promising cost-saving tool for monitoring lake function using remotely-sensed data.