Lagoonal Conditions Research Articles

Lithostratigraphy and Limestone Microfacies of the Jafnayn Formation (Paleocene to Early Eocene, Al-Khod, Sultanate of Oman): Deposition in a Restricted Lagoon with Intervals of Open Marine Conditions

Abstract: We studied the Jafnayn Formation’s lithostratigraphy, microfacies, depositional environment, and uncertain presence of the Paleocene/Eocene boundary and present the first detailed analysis of a 127-m-thick section using the standard microfacies (SMF)/facies zone (FZ) system. The formation is dominated by foraminiferal grainstones and packstones of SMF 18-FOR, followed by peloidal grainstones and packstones of SMF 16. Coral-red algae floatstones of SMF 8 occur sporadically. SMF 18-DASY appears only once. SMF 16 and 18-FOR suggest a restricted lagoon, whereas SMF 8 and 18-DASY reflect episodic open marine lagoonal conditions. The section consists of four lithostratigraphic units. Considerable detrital quartz near the base (Unit 1: 22%; sand, sandstone, conglomerate) and top (Unit 4: 40%; sand) coincides with a restricted lagoon affected by near-shore processes (base) and near-shore conditions (top). Open marine conditions show an elevated bioclast diversity in units 3 and 4. Unit 2 displays very thickly-bedded limestones of the restricted lagoon. The lagoon barriers likely consist of foraminifera and other allochems that were reworked from the lagoon to form shoals. Coral remains in units 3 and 4 suggest that coral build-ups in the upper part of the formation protected the lagoon as well. The early Eocene age of several basal Alveolina species in the lowermost 9 m of the studied section indicates that the section is incomplete, with the late Paleocene part and meters-thick basal yellow marl missing. The Paleocene/Eocene boundary is unexposed.

Hydrologically sensitive carbonates: Tectonic and groundwater controls on synrift sedimentation in the Late Jurassic–Early Cretaceous of the western Cameros Basin, Northern Spain

AbstractBasin hydrology and subcrop are key controls on carbonate sedimentation in continental basins. Hydrologically sensitive carbonates can record groundwater fluctuations within an aquifer in deep time. Late Jurassic extension, footwall uplift, erosion and karstification of marine Jurassic carbonates in the western Cameros Basin (Spain) saw deposition of ?Upper Kimmeridgian‐Tithonian syntectonic alluvial fan deposits (Señora de Brezales Formation). Biogenic laminar calcretes and phreatophytic rhizocretions record roots exploiting capillary fringe groundwater. Progressive infill of rift topography and footwall erosion lowered sedimentary gradients and clastic supply during deposition of the ?Tithonian–Berriasian Rupelo Formation. Distal alluvial marls (Las Viñas Member) contain charophytes, with 2 m thick carbonate lenses at the top reflecting intermittent rise of groundwater in ponds on the basin floor. Stacked palustrine limestones with rare charophytes and laminar calcretes (Ladera Member) record overstep of seasonal carbonate wetlands onto basin margins and footwall highs with intense pedogenetic modification during lengthy seasonal exposure. Overlying Berriasian charophyte‐ostracod wackestones, displaying microkarst cavities and interbedded intraclastic conglomerates, with vivianite sauropod bones, footprints and polygonal desiccation cracks at the top (Mambrillas de Lara Member) record open lacustrine conditions with limited subaerial exposure and high water tables. Desiccation‐cracked limestones and marls with correlatable evaporite horizons (Rio Cabrera Member) contain marginal marine foraminifera and dasycladaceae at the top. Lagoonal conditions reflected transgression to seaward and intermittent marine connection via the Basco‐Cantabrian Basin. The distribution and thicknesses of hydrologically sensitive carbonates reflected onlap onto a faulted and karstified marine Jurassic carbonate pediment and the subtle influence on hydroperiod of fault (and potentially localised Triassic salt) controlled differential subsidence and transgressive groundwater rise. Hydrological facies evolution reflects progressive basin infilling and eustasy beyond. Transitions in this continental succession from clastic to carbonate facies and from closed to open hydrology record hydrological change over time rather than contemporaneous deposition under Walther's Law.

A multi-proxy approach to explore paleoenvironmental oscillations and chronostratigraphy of Thanetian carbonates in Eastern Tethys, Pakistan

This study is the first to incorporate geochemical analyses alongside traditional micropaleontological and sedimentological methods to redefine the chronostratigraphy, paleoenvironmental history and regional correlation of the Late Paleocene Lockhart Limestone from four distinct sections covering the entire Upper Indus Basin. Based on the age diagnostic foraminiferal species, we report SBZ3 to SBZ4 (Thanetian age) for the Lockhart Limestone in contrast to previous studies restricting it to SBZ3. The interpretation of eight distinct microfacies reveals a progressively shallowing upward sequence, indicating a dynamic depositional environment with varying energy levels and water depths ranging from inner, middle, to outer ramp settings. Mineralogically, the formation is predominantly composed of calcite, with quartz being the next significant component. Geochemical analyses, specifically the major oxide results, highlight that CaO is the primary constituent, while notable amounts of SiO2, Fe2O3, Al2O3, and MgO are present. The low proportions of SiO2 and Al2O3, combined with their negative correlation with CaO, and high Mg/Ca ratio suggest limited siliciclastic input during the precipitation of limestone that supports the shallow marine depositional environment inferred from the facies analysis. Trace elemental ratios, indicating predominantly oxic conditions, also align with the well-oxygenated waters of a shallow marine setting. Paleosalinity and paleotemperature indicators suggest normal marine and moderate paleotemperature conditions for limestone facies, with lagoonal conditions for shale facies, which is consistent with the interpreted shallow marine environment. The low Total Organic Carbon (TOC) content indicates the presence of type-III and IV organic matter, derived from recycling or continental sources, suggesting limited organic matter preservation in the depositional setting. The clay mineralogy suggests mainly humid conditions with occasional warm climates and alternating humid and arid periods. The deposition of the marine facies of Lockhart Limestone marks the transgression of the Eastern Tethys following a regressive episode that deposited the continental facies of the Hangu Formation, influenced by evolving paleoclimate and geomorphology under Paleo-Tethys tectonism.

Stratigraphy and microfacies analysis of the Lower–Middle Jurassic platform carbonate succession in the Yeşilovacık (Mersin) area (Central Taurides, S Turkiye)

This study covers the detailed analyses of stratigraphy, microfacies and depositional environments of the Lower-Middle Jurassic carbonate succession in the southern part of Anatolide-Tauride Carbonate Platform in southern Türkiye (south of Central Taurides, around Yeşilovacık). The carbonate succession is composed of the Dibekli and Tokmar formations, and the Işıklıkızıtepe Member which forms the base of the Tokmar Formation. The Dibekli Formation (Upper Sinemurian-Lower Bajocian) is composed of limestones intercalated with intraformational conglomerates in the lower parts, with dolostones in the lower to middle parts and with oncoidal limestones in middle to upper parts. The Işıklıkızıtepe Member (Upper Bajocian) starts with basal conglomerates containing brown-red- iron nodules and consists of sandstone and sandy-clayey limestone alternations. These are overlain by thin limestone layers passing upward to thick-bedded dolostones of the Tokmar Formation (Upper Bajocian–Bathonian). The middle to upper parts of the formation are composed of sandy, oncoidal limestones rich in bivalve shells and end with dolostones-limestone alternations in the upper part. According to microfacies analyzes and litho-bio content; Late Sinemurian-Plesbachian is a period that low-to moderate-energy, restricted inner platform conditions dominated the depositional environments. The presence of comman intraformational conglomerates shows tectonic activity from time to time in this period. The Toarcian to the earliest Bajocian, low-energy intertidal and shallow lagoon conditions dominated the depositional environments, and in the Early Bajocian, the depositional environment changed to a high-energy sand shoal and above wave base environment. The basal conglomerate consisting of brown-red, iron-noduler and bauxite grains at the base of the earliest Late Bajocian indicates a short-term emersion and continued under low energy shallow lagoonal conditions during the Late Bajocian–Bathonian. Environmental changes during the Toarcian to Early Bajocian, and the earliest Late Bajocian short-term emersion, and the environmental changes during the Late Bajocian-Bathonian are interpreted to short-term sea level changes. Yeşilovacık Lower-Middle Jurassic carbonate sequences are correlated with those in peri-Mediterranean platforms. They show close similarities to the other Tauride carbonate sequences in terms of stratigraphy and facies. Paleontological and sedimentological analyses enable us to reconstruct a detailed dating and paleoenvironmental evolution for the Yeşilovacık carbonate deposits during the Lower-Middle Jurassic period.

Antecedent bedrock control on the sediment-starved continental shelf of south/central Namibia

The Namibian inner continental shelf immediately north of the Orange River delta is sediment-poor, dominated by outcrop of metamorphic basement with a scarcity of sandy cover. Using a combination of high and very-high resolution geophysical tools and drill cores, an examination of the evolution of the inner shelf stratigraphy and geomorphology shows persistent antecedent bedrock controls throughout the Pleistocene.The bedrock of the inner shelf comprises schists, in places overlain by Eocene sandstones, over which a subaerial unconformity (H1) formed in the late Oligocene. H1 was repeatedly re-occupied during succeeding transgressive/regressive cycles, creating a backstop for erosion/sedimentation and creating a repeating topography on which transgressions took place. Apart from localised development of Miocene phosphatic limestones, little sediment has since accumulated. Large-scale karst weathering of the limestones reflects several periods of subaerial exposure most recently during the Quaternary lowstands of sea level including MIS 2 and 1.Thin Quaternary cover, housed within depressions on the H1 surface is predominantly preserved in the lee of bedrock ridges, behind which shallow and occasionally hypersaline lagoonal conditions developed. These preserved back-barrier sedimentary sequences developed post-MIS 5 (previous phases were either eroded/weathered or not deposited). Dolomite precipitation occurred when sea-level stabilised at −75 m 37,000 cal BP and restricted lagoonal conditions prevailed.Currently, there are very limited MIS 2/1 deposits preserved above H1. The absence of thick sand cover, adjacent to and downdrift of the Orange River delta, suggests that the onshore diversion of sand that currently supplies the Namib Sand Sea also operated during lower sea levels, related to the bedrock control and lack of accommodation in surface H1. This persistent onshore diversion of sediment throughout the Quaternary has implications for sediment source as well as budget calculations for the Namib Sand Sea.

Palaeoenvironmental changes in eastern Crimea over the last 7600 years inferred from a multi-proxy study of a sediment archive from Lake Chokrak

The study presents a multi-proxy archive from hypersaline coastal Lake Chokrak in eastern Crimea, a steppe region which is particularly susceptible to moisture fluctuations. XRF, organic carbon and nitrogen, and palynological analyses are used to reconstruct the evolution of the lake and landscape development around it and to provide information about the driving forces behind those changes, such as climate and human impact. The findings show that the basin was an open marine bay between 7640 and 5500 cal. yr BP. After 5500 cal. yr BP, the low rates of sea-level rise led to gradual infilling of the basin. The prevalence of mesic herb-grass steppe around the site, as well as the expansion of broad-leaved trees in the Crimean mountains, indicate relatively warm and humid climate conditions during the Mid-Holocene. Elemental proxies and marine NPPs point to a transition to lagoonal conditions from 4270 cal. yr BP. The progressive shallowing of the basin was accompanied by the spread of more xeric Artemisia-grass steppe, which suggests a shift to arid conditions. The occurrence of cereal pollen from 3660 cal. yr BP may indicate the first signs of cultivation in the study area. The active build-up of a sand barrier from 3000 cal. yr BP led to a gradual separation of the basin from the sea. By 900 cal. yr BP, the fully-formed sand barrier resulted in a transformation of the lagoon into a hypersaline lake. The closing of the lagoon was accompanied by an expansion of halophytic communities around the site. Human-induced vegetation changes became more pronounced with the development of agrarian and pastoral activities around the site from ca. 750 cal. yr BP.

Lagoonal Microfacies, Lithostratigraphy, Correlation and Shale Migration of the Basal Middle Eocene Seeb Formation (Rusayl Embayment, Sultanate of Oman)

The study improves the understanding of the basal part of the Eocene Seeb Formation of Oman, informally known as “Unit 1”, in terms of microfacies, lithostratigraphy and shale migration within the context of regional tectonics. We logged four sections bed-by-bed over a distance of 8.3 km, collected samples and analyzed thin-sections as well as XRD samples. For the first time, the microfacies and stratigraphic correlation of the lowermost part of the limestone-dominated Seeb Formation were studied in detail. In the analyzed area, Unit 1 is ~20 to 40 m thick, with the thickness increasing to the SE. In the upper part of Unit 1 is a laterally continuous shale horizon. The limestones of Unit 1 contain mostly packstones and grainstones. The dominant standard microfacies types are SMF 18-FOR and SMF 16. The former is dominated by benthic foraminifera, and the latter by peloids. Both SMFs indicate restricted lagoonal conditions. Foraminifera are common in Unit 1 and indicate a middle Eocene age. Considering the abundance of encountered foraminiferal bioclasts, it appears probable that the lagoon barrier was mainly composed of foraminiferal tests. Gutter casts, slumps and debrites indicate an active, partly unstable syndepositional slope, which was likely initially created by uplift of the Saih Hatat Dome and Jabal Nakhl Subdome. Differential regional uplift due to a more pronounced overall doming in the NW (Jabal Nakhl Subdome) than in the SE (Saih Hatat Dome) explains more accommodation space and greater thickness towards the SE. For the first time, we report visco-plastic shale migration/intrusion within the Seeb Formation, related to a shale horizon of Unit 1. This shale locally migrated as indicated by (1) local thickness variations, (2) detached limestone boulders floating in the shale, (3) limestone beds that have been cut-off by the shale and (4) dragged by the shale (5) an upward shale intrusion/injection which then spread parallelly to bedding similar to a salt tongue and (6) tilting overlying limestones. We suggest that shale migration is related to post-“mid”-Eocene E-W convergence between Arabia and India and to faulting or to the second, late Paleogene/early Neogene, faulting interval of the Frontal Range Fault. The shale horizon in the upper part of Unit 1 is a marker bed, which can be correlated across the study area.

Modeling Benthic Community Settlement and Recruitment on Living Dock Restoration Mats

An increase in population along the Indian River Lagoon has led to eutrophication, a decline in water quality, and overall degradation. The Living Docks program is a citizen–science initiative started at the Florida Institute of Technology for lagoon restoration. Public and private docks are volunteered to become Living Docks, where oyster mats are attached to dock pilings to provide a natural substrate for benthic organism growth. The community development on the oyster mats boosts water filtration to improve overall water quality and combat anthropogenic effects on the lagoon. The purpose of this project was to model benthic settlement and recruitment of prominent organisms on the Living Dock oyster mats at four research sites with specific environmental factors (e.g., temperature, salinity, turbidity, and pH). Beta regression models for recruitment and settlement were created for five of the more dominant organisms observed: oyster, barnacle, sponge, tubeworm, and encrusting bryozoan. The results of the modeling indicated that the settlement was influenced by pH, salinity, dock location, and turbidity, while recruitment was influenced by pH, salinity, dock location, and immersion time. This project provides insight into how lagoon conditions surrounding the Living Docks impact benthic growth and can aid in IRL restoration.

Genetic structure of pike (Esox lucius Linnaeus, 1758) populations along the Polish coast of the southern Baltic Sea: Comparison to Danish brackish population

The northern pike is a species of eco-evolutionary and socio-economic importance, originally freshwater but adapted to the conditions of brackish bays, lagoons and rivers flowing into seas. It was widespread along the entire coast of the Baltic Sea. A drastic decline in the stocks of Baltic pike in the 20th century caused large-scale stocking activities in the area, often based on non-native material from more abundant freshwater pike populations. Research into the genetic variation and structure of this species in the Baltic Sea is necessary as part of the key knowledge required for the management and conservation of the species. Applying eleven microsatellite markers, we analysed the genetic structure of 495 Baltic Sea pike sampled along the Polish coastal waters and including distant Danish population, collected in Præstø Fjord, in south-eastern Zealand Island. Using traditional F-statistics and a Bayesian approach we confirmed the presence of genetic structure within the analysed region. It was most likely the result of both evolutionary forces and anthropogenic factors. The analyses showed that genetic differentiation observed for the Danish and the others populations generally increased with the geographical distance, populations from the Puck Bay were distinct from the other, and the populations related to the Vistula delta and the Vistula itself form a strong common cluster. Our results contribute to current knowledge on the population genetics of Baltic Sea pike in a previously unstudied area and are in line with calls for on-going conservation, management and restoration activities for coastal Baltic pike to preserve existing genetic differences between different populations and avoid activities that potentially increase genetic homogenization.

Holocene Paleoenvironmental Evolution of a Semi-Enclosed Shallow Aegean Basin: A Combination of Seismic Stratigraphy and Sediment Core Proxies

The island of Astypalea (Greece), known for its rich and pristine archeological sites, encompasses a semi-enclosed silled basin that has been very susceptible to global sea levels and regional climate changes due to its relatively modern shallow sill of 4.7 m water deep that connects the Vathy bay with the adjacent Aegean Sea. To identify the causal relationship between regional climate, sea-level trajectories, and environmental change and their potential impact on hominine habitats on the island, we investigated a high-resolution seismic profile together with sediment, stable isotope, geochemical, and biotic proxies retrieved from a marine sediment core (ASTC1). Our results show that the basin was once isolated, and a marine inundation occurred at around 7.3 ka BP, which is older than expected, based on global sea level reconstructions. The entire transition from isolation to full marine conditions was accomplished in three major phases: (1) non-marine isolated conditions between 9–7.3 ka BP, (2) semi-isolated hypersaline marsh and lagoonal conditions between 7.3 and 4.1 ka BP, and (3) semi-isolated shallow marine conditions of today (4.1 ka BP to present). High water alkalinity, elevated organic content, and heavier isotopic signals indicate relatively arid conditions in the region that favored Sr-rich carbonate precipitation within the 7.3–6 ka BP interval. On the other hand, freshwater biota, along with a high Corg/N ratio and lighter isotopic signal, showed wetter conditions, at least for the intervals 8–7.3 ka and 6–5.4 ka BP, in contrast to the aridification trend seen as 4.1 ka to present. Finally, the hominine habitat evolution at around 6 ka BP might be attributed to the wetter conditions and the freshwater source provided by the bay at that time.

Holocene coastal evolution and environmental changes in the lower Río Guadiaro valley, with particular focus on the Bronze to Iron Age harbour ‘Montilla’ of Los Castillejos de Alcorrín (Málaga, Andalusia, Spain)

AbstractPhoenicians were the first to systematically develop the area surrounding the Strait of Gibraltar at the end of the 9th century B.C. Following pioneering studies in the Río Guadiaro estuary (Málaga/Cádiz) in the 1980s, a German‐Spanish cooperation project focussed on the role of indigenous people in the Phoenician colonisation trading networks at Los Castillejos de Alcorrín (Manilva, Málaga), one of the most important Early Iron Age settlements in southwestern Iberia. In the recent past, combined with systematic archaeological surveys, geoarchaeological research embedded in the interdisciplinary project ‘Archeostraits’ aimed at (i) deciphering palaeoenvironmental and coastal changes in the surroundings of Los Castillejos de Alcorrín throughout the mid‐ to late Holocene; (ii) constraining palaeoenvironmental conditions during early Phoenician colonisation; and (iii) better understanding human–environment interactions during the Final Bronze and Early Iron Age (i.e., end of 9th and 8th centuries B.C.). Coring transects along the Río Guadiaro allowed for differentiating successive palaeoenvironments and for establishing a chrono‐stratigraphy for the Holocene sedimentary infill of the valley. Based on these results, the deposition of shallow marine sands, overlying deltaic deposits of alternating sand and mud, and the subsequent development of lagoonal conditions in the lower Guadiaro valley took place before the Phoenicians established the first settlements along the coast.

Shaping coral traits: plasticity more than filtering

The structure of an ecosystem is usually determined by the shape of the organisms that build it, commonly known as ecosystem engineers. Understanding to what extent plasticity and environmental filtering determine variation in the physical structure of ecosystem engineers is necessary to predict how ecosystem structure may change. Here, we explored coral survival and the plasticity of morphological traits that are critical for habitat provision in coral reefs. We conducted a reciprocal clonal transplant experiment in which branching corals from the genera Porites and Acropora were moved to and from a deep and a shallow site within a lagoon in the Maldives. Survival and trait analyses revealed that transplant destination consistently induced the strongest changes, particularly among Acropora spp. The origin of the corals had only marginal effects on some of the traits. We also detected variation in the way individuals from the same species and site differentiate in their shape, showing that traits linked to habitat provision are phenotypically plastic. The results suggest that in the quite common lagoonal conditions studied here, coral phenotypic plasticity plays a stronger role than environmental filtering, in determining the zonation of coral morphologies, and consequently the habitats they provide for other organisms.

Quantification of antibiotic resistance genes and mobile genetic in dairy manure.

BackgroundAntibiotic resistance genes (ARGs) are considered to be emerging environmental contaminants of concern potentially posing risks to human and animal health, and this research studied the prevalence of antimicrobial resistance in dairy manure.MethodsThis study is focused on investigating prevalence of ARGs in California dairy farm manure under current common different manure management. A total of 33 manure samples were collected from multiple manure treatment conditions: (1) flushed manure (FM), (2) fresh pile (FP), (3) compost pile (CP), (4) primary lagoon (PL), and (5) secondary lagoon (SL). After DNA extraction, all fecal samples were screened by PCR for the presence of eight ARGs: four sulfonamide ARGs (sulI, sulII, sulIII, sulA), two tetracycline ARGs (tetW, tetO), two macrolide-lincosamide-streptogramin B (MLSB) ARGs (ermB, ermF). Samples were also screened for two mobile genetic elements (MGEs) (intI1, tnpA), which are responsible for dissemination of ARGs. Quantitative PCR was then used to screen all samples for five ARGs (sulII, tetW, ermF, tnpA and intI1).ResultsPrevalence of genes varied among sample types, but all genes were detectable in different manure types. Results showed that liquid-solid separation, piling, and lagoon conditions had limited effects on reducing ARGs and MGEs, and the effect was only found significant on tetW (p = 0.01). Besides, network analysis indicated that sulII was associated with tnpA (p < 0.05), and Psychrobacter and Pseudomonas as opportunistic human pathogens, were potential ARG/MGE hosts (p < 0.05). This research indicated current different manure management practices in California dairy farms has limited effects on reducing ARGs and MGEs. Improvement of different manure management in dairy farms is thus important to mitigate dissemination of ARGs into the environment.

Microfacies Analysis, Diagenetic Aspects and Depositional Environments of the Oligocene-Miocene Rock Units Exposed at Al Bardia Coastal Area, North East Tobruk City, Libya

The Oligocene-Miocene carbonate succession exposed at Wadi Shamas and Wadi Rizk in the coastal area of Al Bardia was investigated to determine their depositional environments. This succession includes from base to top; Al Khowaymat, Al Faidiyah and Al Jaghbub Formation s. It was found that Al Khowaymat Formation is characterized by three microfacies associations (foraminiferal bioclastic( packstone, Wackestone and floatstone) rich with foraminifera and bivalves pointing to deposition within intertidal to lagoonal conditions related to regressed sea level with relatively high agitation due to the presence of bivalves and shell banks. Al Faidiyah Formation has three microfacies associations (packstone, floatstone and grainstone) rich in algae, bivalves, bryozoans and foraminifera indicating deposition in prograding advanced sea with deeper sub-environments from shallow energetic. Al Jaghbub Formation have four microfacies associations (packstone, floatstone, dolomitic wackestone and rudstone) rich with bryozoa, algae, bivalves and foraminifera, indicating deposition in tidal flats to mid ramp conditions. Micritization, dissolution and replacement are the main diagenetic processes affected the studied carbonate rocks.

Heavy Metals in the Blood Serum and Feces of Mugger Crocodile (Crocodylus palustris) in Sistan and Baluchistan Province, Iran.

This study was conducted to measure concentrations of cadmium (Cd), lead (Pb), arsenic (As), mercury (Hg), copper (Cu), chromium (Cr), and iron (Fe) in blood serum and feces of mugger crocodile (Crocodylus palustris) and in water and sediment of their habitat (Negour site, Chabahar County in Sistan and Baluchistan Province, Iran). Heavy metal contents in crocodile serum and feces, water, and sediments were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES spectrometer-Spectro Genesis). The highest levels of heavy metals in the mugger crocodile's serum were Fe > Hg > Pb > Cr > Cu > Cd > As, respectively. The total metal concentrations in the feces exhibited the following decreasing order from Fe > As > Pb = Cr > Hg > Cd > Cu. The highest average levels of heavy metals in water were Hg > Fe > As > Pb > Cu > Cr > Cd respectively and As > Fe > Pb > Cu > Hg > Cd > Cr in the sediment of lagoons. A significant difference was observed in the concentration of Fe and Pb between male and female crocodiles. Moreover, correlation analysis indicated significant negative correlations between sex and bioconcentrations of Cd, Fe, Pb, As, and Hg in the serum. In conclusion, this study showed that the environmental conditions of Negour lagoons are not suitable for crocodiles regarding habitat health and water quality. Furthermore, since these lagoons and fish are the most important sources of drinking water and seafood for the residents of the area, contamination of these pools with heavy metals can also threaten people's health.

The challenge of assessing the proper functioning conditions of coastal lagoons to improve their future management

The sustainable management of coastal lagoon hydrosystems is a key issue for the socio-economic and environmental development of many coastal areas worldwide. Often maintained by direct or indirect groundwater supplies, they provide a large range of ecosystem services, from which human societies take advantage. Twenty years after its implementation, a large majority of the Mediterranean lagoons have still not reached the “good status” required by the WFD. Several questions then arise as to the representativeness of the WFD indicators or the relevance of the restoration objectives considering the complexity, evolutionary and unpredictable nature of lagoon hydrosystems. This study proposes an innovative, multidisciplinary, long-term approach to define the proper functioning conditions of a costal lagoon hydrosystems, i.e. all the factors that contribute to the functioning of coastal lagoon hydrosystems and the connectivity with other biodiversity reservoirs, be they ecological, hydrological, social or political. By considering the lagoons and all its hydrological, ecological and societal proper functioning conditions over almost 200 years, this approach makes it possible to assess the influence of past natural and anthropogenic disturbances and support the implementation of future relevant hydrosystem-based management plans which have to be coordinated and politically driven. Defining proper restoration and management objectives should ensure that ecological functions are maintained based on current and future ecosystem benefits and uses. Considering the highly unpredictable nature of coastal hydrosystems, the state of an ecosystem should not only be evaluated on ecological or chemical criteria but also take into account socio-economic and political indicators. The implementation of the 3rd river basin management plan of the WFD as soon as 2022 could be the appropriate occasion to reassess the restoration objectives towards more realistic goals and to give more significance to the definition of the “resilience capacity” of water bodies in place of inappropriate restricted restauration objectives.

AN OVERVIEW ON THE LATE MIOCENE VERTEBRATES FROM THE FISSURE FILLINGS OF MONTICINO QUARRY (BRISIGHELLA, ITALY), WITH NEW DATA ON NON-MAMMALIAN TAXA

The latest Messinian Monticino Quarry fissure fillings, near Brisighella in Italy, are well known for their diverse mammal fauna. Conversely, little is known about other vertebrates from this rich site. Beside presenting an overview of the mammals, here we describe fish, amphibian, reptile, and bird remains, identifying three, eight, at least 18, and five taxa, respectively. Some of these represent the oldest occurrences either worldwide (an erycine snake related to Eryx jaculus, Malpolon, and otidids birds) or locally (common toads in the Apennine Peninsula and eremiadine lacertids in Europe, the latter being also the only known occurrence of these lizards in Italy). Altogether, the vertebrate fauna from Monticino Quarry is indicative of a complex palaeoenvironment, which was warm and somehow dry. Water bodies were indeed present though, as well as a patchy set of habitats with loose or sandy soils, rocky outcrops, open biotopes, and (even though maybe to a lesser extent) wooded areas. Furthermore, estuarine or lagoonal conditions were likely present near the depositional site. From a palaeobiogeographical point of view, the non-mammal vertebrate assemblage from Monticino Quarry shows considerable Afro-Asian affinities at least for some components. In the context of the Italian late Miocene continental vertebrate assemblages, that of Monticino is remarkably similar to those from the Piedmont Basin, especially if compared with the Tusco-Sardinian area, Apulian Platform, Calabrian Arc and Sicily. This is consistent with the palaeobiogeographic scenario defined by mammal assemblages, with northcentral Italy, Tuscany and Sardinia, Apulian Platform, and Calabria and Sicily representing separated bioprovinces.

Late Glacial Marine Transgression and Ecosystem Response in the Landlocked Elefsis Bay (Northern Saronikos Gulf, Greece)

Coastal landscapes are sensitive to changes due to the interplay between surface and submarine geological processes, climate variability, and relative sea level fluctuations. The sedimentary archives of such marginal areas record in detail the complex evolution of the paleoenvironment and the diachronic biota response. The Elefsis Bay is nowadays a landlocked shallow marine basin with restricted communication to the open Saronikos Gulf. A multi-proxy investigation of a high-resolution sediment core recovered from the deepest part of the basin offered a unique opportunity to record the paleoenvironmental and aquatic ecosystem response to climate and glacioeustatic sea level changes since the Late Glacial marine transgression. The retrieved sedimentary deposits, subjected to thorough palynological (pollen, non-pollen palynomorphs, dinoflagellates), micropaleontological (benthic foraminifera, calcareous nannoplankton, ostracods), and mollusc analyses, indicates isolation of the Elefsis Bay from the Saronikos Gulf and the occurrence of a shallow freshwater paleolake since at least 13,500 cal BP, while after 11,350 cal BP the transition towards lagoon conditions is evidenced. The marine transgression in the Elefsis Bay is dated at 7500 cal BP, marking the establishment of the modern marine realm.

The Genomic Capabilities of Microbial Communities Track Seasonal Variation in Environmental Conditions of Arctic Lagoons.

In contrast to temperate systems, Arctic lagoons that span the Alaska Beaufort Sea coast face extreme seasonality. Nine months of ice cover up to ∼1.7 m thick is followed by a spring thaw that introduces an enormous pulse of freshwater, nutrients, and organic matter into these lagoons over a relatively brief 2–3 week period. Prokaryotic communities link these subsidies to lagoon food webs through nutrient uptake, heterotrophic production, and other biogeochemical processes, but little is known about how the genomic capabilities of these communities respond to seasonal variability. Replicate water samples from two lagoons and one coastal site near Kaktovik, AK were collected in April (full ice cover), June (ice break up), and August (open water) to represent winter, spring, and summer, respectively. Samples were size fractionated to distinguish free-living and particle-attached microbial communities. Multivariate analysis of metagenomes indicated that seasonal variability in gene abundances was greater than variability between size fractions and sites, and that June differed significantly from the other months. Spring (June) gene abundances reflected the high input of watershed-sourced nutrients and organic matter via spring thaw, featuring indicator genes for denitrification possibly linked to greater organic carbon availability, and genes for processing phytoplankton-derived organic matter associated with spring blooms. Summer featured fewer indicator genes, but had increased abundances of anoxygenic photosynthesis genes, possibly associated with elevated light availability. Winter (April) gene abundances suggested low energy inputs and autotrophic bacterial metabolism, featuring indicator genes for chemoautotrophic carbon fixation, methane metabolism, and nitrification. Winter indicator genes for nitrification belonged to Thaumarchaeota and Nitrosomonadales, suggesting these organisms play an important role in oxidizing ammonium during the under-ice period. This study shows that high latitude estuarine microbial assemblages shift metabolic capabilities as they change phylogenetic composition between these extreme seasons, providing evidence that these communities may be resilient to large hydrological events in a rapidly changing Arctic.

Depositional setup of the faunal coal balls from Bichom Formation of Lower Gondwana Group of Arunachal Himalaya: insights from EPMA and Raman Spectroscopy

An attempt is made to study the composition of the carbonate minerals and silica in the faunal coal balls of Lower Gondwana Group of rocks of Arunachal Himalaya using Electron Probe Micro Analyser (EPMA) and Raman Spectrometer (RS). It is observed that, the carbonates developed in the coal balls contain Fe, Mg and Mn which corresponds to the concentration of dolomite and indicates a relatively high salinity in the sedimentary environment or of the formation water. Raman spectrum of silica indicates the presence of hydroxylated nanometre sized α quartz- chalcedony in the coal balls. We construe the incorporation of electrolyte rich solution in the coal swamps near sea shore took place due to repeated influx of sea water in lagoonal condition facilitating precipitation of chalcedony in a restricted alkaline environment thus created.