Background: This study aimed to assess the Vitamin D status among reproductive-age women in Quetta. No notable studies in recent years have evaluated Vitamin D levels in this population. This study seeks to report the Vitamin D status in this demographic and set the foundation for future strategies to address this issue. Methodology: This cross-sectional study was conducted on women aged 15-49 years. Demographic information was collected using a proforma, and blood samples for Vitamin D assessment were obtained with consent by trained lady health visitors. Data were analyzed using SPSS 20 and Microsoft Excel 2010. Results: The results showed that 78.4% of the women were deficient in Vitamin D, 15.3% had insufficiency, 6.4% had sufficient levels, and none had Vitamin D intoxication. Additionally, 61.6% of the participants were illiterate, and 78.4% had a household income of less than Rs 10,000 per month. There was a significant correlation between literacy levels and Vitamin D levels, as well as between income and Vitamin D levels. Conclusion: There is a consistent trend of suboptimal Vitamin D levels among reproductive-age women, as indicated in previous studies. This study concluded that Vitamin D status in this population is alarmingly low, necessitating immediate attention to improve maternal and neonatal health through educational and interventional strategies.