Lactobacillus Sp Research Articles

Oral Lactobacilli Related to Caries Status of Children with Primary Dentition

Oral lactobacilli are members of a group of bacteria implicated in caries progression, although information regarding their transmission, colonization, and caries-associated species is not well established. This study isolated oral lactobacilli from a group of children with primary dentition for determination of Lactobacillus prevalence, detection of Streptococcus mutans, a major pathogen of caries initiation, and dental caries status of the children. Species of Lactobacillus isolates were determined from examination of 16S rDNA sequences. Subsequently, the most prevalent species was evaluated for involvement in caries status, and binding ability to type I collagen of all Lactobacillus isolates was determined in association with caries status. Multilocus sequence typing (MLST) of eleven loci was carried out to study strains of the predominant Lactobacillus sp. The detection of oral lactobacilli together with S. mutans was significantly associated with the highest dental caries indices, but there was no involvement of collagen-binding properties of Lactobacillus isolates in caries status. Lactobacillus fermentum was the most prevalent, and its presence was related to high scores of caries indices. MLST analysis of L. fermentum population could not specify a particular clone associated with caries status, but revealed sharing of identical L. fermentum strains among children in the same classrooms. Taken together, the data contributed useful information on the role of oral lactobacilli, in particular L. fermentum in dental caries.

THE EFFECT OF Streptococcus sp AND Lactobacillus sp ATTACHMENT ON THE LEVEL OF FLUORIDE ION SOLUBILITY IN GIC RESTORATION

Background: Glass ionomer cement (GIC) is a restoration material that has the ability to release Fluoride ion. The Extrinsic factor that affect the solubility of fluoride is low or acidic pH. Streptococcus sp and Lactobacillus sp are asiduric and acidogenic bacteria which are resistant to acidic conditions and may produce acid. A lower pH (acid) causes higher level of fluoride to be released. Objective: This study was to analyze the effect of Streptococcus sp and Lactobacillus sp attachment on solubility of fluoride level on GIC restoration Method: Twenty-one specimens (5mm diameter x 2mm thickness; n = 7 / group) were fabricated with GIC. The treatment group (Streptococcus sp and Lactobacillus sp) while the control group (Artificial Saliva) was immersion for 7 days in an incubator 37oC. Solubility of fluoride was measured using a pH meter Lutron (208) TL Lutron (Taiwan). Result: One Way Anova and Post Hoc Bonferonni showed that there were significant differences between the treatment groups of Streptococcus sp (5.13 ± 0.86ppm) and Lactobacillus sp (6, 02 ± 0.79ppm) with artificial Saliva group controls (3.03±0,56ppm). Conclusion: There was an effect of Streptococcus sp and Lactobacillus sp bacteria attachment on the solubility of fluoride from the GIC restoration.

USE OF LACTIC ACID BACTERIA IN PEANUT SEED TREATMENT

Three lactic acid bacteria (LAB) strains, Lactobacillus sp. L5, Lactobacillus sp. L3, Lactobacillus sp. L2N, isolated from Vietnamese traditional Nem chua grew well in cabbage broth supplemented with 12 g/L glucose and 15 g/L peptone and showed inhibitory activity ranging from 40% to 44% against Aspergillus sp. CDP isolated from mould contaminated peanuts, while Daconil 75WP – a fungicide compounds – as a positive control showed only 26.9%. LAB strains also displayed Indole-acetic acid (IAA) production, P-solubilization and biofilm formation. Soaking seeds in separate cabbage based culture broth of three LAB strains with/without heat treatment and mixed culture broths at the ratio 1:1:1 with the total bacterial count 108cfu/mL exhibited the antifungal activity of mixed cultures in both cases with or without bacterial culture heat treatment. Soaking seeds in the same mixed bacterial cultures without heat treatment increased seed germination and vigor index, compared to the control seeds without any treatment and those treated with fungicide compounds. After 75 days of sowing the length and total fresh weight of LAB-treated peanut plants increased by 22.4 % and 99.6%, higher than that of Daconil treated ones with only 15.9% and 59.7% increment. Moreover, the fresh yield of peanut pegs increased 2.5 times, compared to those of untreated and Daconil treated seeds. This study suggested that seed treatment with LAB is a novel technology towards organic farming to replace fungicide used in conventional agriculture.

Exploring Probiotic Activity of Lactobacillus sp. Isolated from Indigenous Breeds of Cattle Milk and Fecal Samples in Bhatan Village, MH., IN.

Probiotics are defined as live organisms that are able to confer health benefits to the host by improving their intestinal microbial balance. In the last decade, there has been an increasing interest to reveal health benefits associated with them. The objective of this study was to isolate indigenous probiotic organisms and assess their probiotic activity and therapeutic characteristics. The isolates were identified as Lactobacillus fermentum (isolates 2, 4, 6, 7, 8, and 9), Lactobacillus salivarius (isolate 13), and Lactobacillus plantarum (isolates 32 and 36). Five isolates showed growth at pH 2.5, while all isolates could grow at pH 8.5. All isolates showed good growth upto 5% NaCl concentration while two isolates showed growth in 7% NaCl concentration. All the isolates were susceptible to most of the broad-spectrum antibiotics. Cell-free suspensions from the isolates showed antimicrobial activity against the tested strains of Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Staphylococcus aureus. Two of the isolates 32 and 36 showed good revival after long-term storage, without any change in the morphology. Hence among all the other isolates these two isolates could have a good marketable potential. These strains can further be formulated into a probiotic drink that can be used as a health supplement.

On-farm evaluation of three strains of <i>Lactobacillus</i> sp. based probiotics on the hematology and serum lipid profile of local toms

A 16-week study was conducted to determine the effect of three strains of Lactobacillus sp based probiotics on hematological indices and serum lipid profile of local toms. A total of 80-day-old local male poults were randomly assigned to four treatments (T1, T2, T3 and T4) in a completely randomized design with 20 birds per treatment. Three strains of Lactobacillus namely L. delbrueckii sub sp. bulgaricus , L. acidophilus and L. sporogens were incorporated in the drinking water of T2, T3, and T4 respectively at 0.5 ml/L. T1 served as control. Each treatment was replicated twice with 10 birds per replicate. Feed and water were provided ad libitum . Results showed that the effect of the different strains of Lactobacillus sp. on hemoglobin, red blood cell count, white blood cell count, serum triglycerides, cholesterol, high-density lipoproteins and low-density lipoproteins were significant ( p < 0.05). Birds on probiotics inclusion had least values for white blood cell counts, low-density lipoproteins, cholesterol and serum triglycerides with T4 recording the most superior values. It was thus concluded that of the three strains of Lactobacillus sp based probiotics used in the study, Lactobacillus sporogens was observed to support superior hematological values with reduced lipid profiles. Keywords: direct-fed microbials, fat accretion, health status, Lactobacillus sp., turkey production

Enhancement of 1,3-propanediol production from industrial by-product by Lactobacillus reuteri CH53

Background1,3-propanediol (1,3-PDO) is the most widely studied value-added product that can be produced by feeding glycerol to bacteria, including Lactobacillus sp. However, previous research reported that L. reuteri only produced small amounts and had low productivity of 1,3-PDO. It is urgent to develop procedures that improve the production and productivity of 1,3-PDO.ResultsWe identified a novel L. reuteri CH53 isolate that efficiently converted glycerol into 1,3-PDO, and performed batch co-fermentation with glycerol and glucose to evaluate its production of 1,3-PDO and other products. We optimized the fermentation conditions and nitrogen sources to increase the productivity. Fed-batch fermentation using corn steep liquor (CSL) as a replacement for beef extract led to 1,3-PDO production (68.32 ± 0.84 g/L) and productivity (1.27 ± 0.02 g/L/h) at optimized conditions (unaerated and 100 rpm). When CSL was used as an alternative nitrogen source, the activity of the vitamin B12-dependent glycerol dehydratase (dhaB) and 1,3-propanediol oxidoreductase (dhaT) increased. Also, the productivity and yield of 1,3-PDO increased as well. These results showed the highest productivity in Lactobacillus species. In addition, hurdle to 1,3-PDO production in this strain were identified via analysis of the half-maximal inhibitory concentration for growth (IC50) of numerous substrates and metabolites.ConclusionsWe used CSL as a low-cost nitrogen source to replace beef extract for 1,3-PDO production in L. reuteri CH53. These cells efficiently utilized crude glycerol and CSL to produce 1,3-PDO. This strain has great promise for the production of 1,3-PDO because it is generally recognized as safe (GRAS) and non-pathogenic. Also, this strain has high productivity and high conversion yield.

Lactobacillus sp as a Probiotic for the Prevention of Clostridium difficile Associated Diarrhea

Background: Antibiotic treatment can disturb the resistance of the gastrointestinal flora to colonization. This may result in complications, the most serious of which is Clostridium difficile associated diarrhea (CDAD). The aim of this study was to determine the effectiveness of probiotics for the prevention of CDAD.Method: The databases used were Scopus, Proquest, CINAHL, Medline, Pubmed and ScienceDirect, limited to having been published in the last 5 years. A literature review followed the keyword search. The keywords used were probiotics, Clostridium difficile, associated, diarrhea, randomized, control and trial using "AND" and “OR”. Twelve trials with 5102 participants were included. Eight trials reported a preventive effect for CDAD using a mixture of 2 strains of lactobacillus, a mixture of 4 combination strains, a mixture of lactobacillus and Saccharomyces or a mixture of Bifidobacterium, Lactobacillus and Saccharomyces.Result: Our findings indicate that probiotics may prevent CDAD. Most probiotics contain a singular strain. The combination with lactobacillus sp was the most effective at preventing CDAD.Conclusion: In addition, 6 out of 8 trials had an in relation to preventing CDAD containing lactobacillus sp. Four studies said that there were some factors that meant that the probiotic could not reduce or prevent the CDAD.

Enumeration of probiotic strains from synbiotic samples produced by Myternak Trading

Natural defense mechanisms of animals by using probiotics, prebiotics, and synbiotics have been seen as an alternative way to improve animal health and to overcome the food-borne diseases among the livestock. This is as the previous use of antibiotics as the growth promoter in the poultry industry has been banned by the European Union. Synbiotic is a combination of probiotics and prebiotics where probiotics are live microorganisms that give a gainful medical advantage on the host whereas prebiotics are nondigestible food ingredients that influence the host by stimulating the development and the action of a set number of microscopic organisms in the colon. This study was conducted to assess the viability of probiotic strains in the synbiotic powder samples from Myternak Trading as well as to see the correlation between the viability of probiotic strains with the amount of its selective prebiotics. Two different types of prebiotics; fructooligosaccharides (FOS) and inulin with seven different compositions of prebiotics had been used in this study. The viability of probiotic strains in synbiotic powder samples were assessed by enumeration of probiotic strains in two different selective media which are TOS and MRS agar. The highest average colony forming units (CFU) for Bifidobacterium sp. can be found in Batch 7 samples (4.10 χ 1011 CFU/ml ) as the volume of FOS is the highest, which is 0.5 mL. While the highest CFU for Lactobacillus sp.can be found in Batch 1 samples (5.560 χ 1011CFU/ml) as the weight of inulin is the highest, which is 5.0 g. These results proved the effects of prebiotic compositions in synbiotics towards the viability of the probiotic strains.. The viable counts of each probiotic strains that contain in the synbiotic powder sample significantly decreases as the amount of selective prebiotic decreases. Therefore, the maximum CFU obtained as the composition of prebiotic increases. Hence it can be concluded that the produced synbiotic powder samples by MyTernak Trading could be used as an alternative feed additive in boosting the livestock health due to the viability of both probiotic strains which are Bifidobacterium sp. and Lactobacillus sp in the synbiotic samples.

VIABILITAS Lactobacillus sp F213 PADA BERBAGAI MINUMAN SARI BUAH PROBIOTIK SELAMA PENYIMPANAN

Lactobacillus sp F213 (LbF213) is a lactic acid bacterium isolated from a healthy baby feces, which has been proven as a probiotic bacteria because it is resistant to low pH, bile salts, digestive enzymes, able to aggregate, attach and colonize the intestine, and interact against E. coli. This study aims to determine the viability of probiotic bacteria LbF213 on various juices during storage. The fruits used in this study were soursop, tamarillo, orange, and pomello. This study used a descriptive comparative method experimental design. The variables observed were total LAB, total sugar, total acid, and pH tested every 3 days from the 1st day to the 12th day in the cold storage. The results showed that the total LAB of LbF213 in tamarillo juice during 12 days of storage tend to be constant compared to soursop, orange, and grapefruit juice whereas total LAB, from the first day of storage was 1.37x106 CFU/ml to 12th day of storage was 1.81x106 CFU / ml with a total sugar value 3.13%, total acid value 1.22%, and pH 3.95.&#x0D; Keywords: fruit juice, Lactobacillus sp F123, cold storage

Manajemen usaha Ayam Pedaging Jantan dan Betina dengan Penambahan Suplemen Organik Cair

This study aims to determine the growth of broiler chickens and analyze the feasibility of broiler farming with the addition of liquid organic supplements. Liquid organic supplements (SOC) or also called probiotics contain Lactobacillus sp, Azetobacter sp, Basiluss sp, vitamins, and minerals. SOC can increase antibodies, reduce stress levels, suppress the onset of disease, help stimulate appetite, and accelerate the growth of broiler chicken. Broilers are divided into 4 treatments female broilers without SOC, male broilers without SOC, female broilers with the addition of SOC, and treatment of male broilers with the addition of SOC. Based on the results and discussion the highest PBB is obtained by male chicken with SOC. Feasibility analysis shows that all treatments are feasible, B/C (3.11) and R/C (3.18) values of treatments added by SOC are higher than treatments without SOC.Keywords: Broiler, Feasibility Analysis, SOC

Isolation And Characterization Of Lactic Acid Bacteria (Lab) From Small Intestine Content Of Duck (Anas Sp.) As A Probiotic Candidate

A group of lactic acid bacteria (LAB) as a probiotic agent have been known to have a beneficial effect on health chicken. The objectives of this study were to isolate, characterize and determine of the LAB which originated from the small intestine content of domestic duck (Anas sp.) for candidate of probiotic. Isolation, selection and identification of bacteria was done using de Man Rogosa Sharpe (MRS) agar + 1% bromocresol purple. Characterization of bacteria based on morphological by Gram staining methods, and identification of biochemical characters using catalase activity, and Triple Sugar Iron Agar (TSIA). The data of isolate confirmed to Bergey’s Manual of Systematic Bacteriology. This research carried out on the Microbiology Laboratory, Microbiology Study Program, Yala Radjabhat University (YRU), Faculty of Science and Agriculture.The results showed that according to physiological and biochemical characteristics in various media, the bacteria had Gram +, catalase -, coccus in shape, and were able to ferment glucose to be lactic acid. Based on the result, it is concluded that the LAB isolate shows a similar characteristic to the member of genus Lactobacillus sp. The isolated bacteria shows characteristic resistance to acidic pH and bile salts so the bacteria were fulfilled as a probiotic candidate.

The exploration and utilization of functional microorganisms isolated from the tropical peatlands

The utilization of peatlands may decline the ecological functions of peatlands as carbon sink, water storage and biodiversity source. Therefore, different scientific approaches are required to increase peat productivity and inhibit decline in the ecological functions of peatlands. This article is aimed to provide information on researches that have been carried out in tropical peats in the South Kalimantan Province. The exploration and the potential utilization of microbes was reviewed from several studies conducted in the Desa Landasan Ulin Utara (LaURa), Kota Banjarbaru, South Kalimantan Province. Peat research carried out in the Desa LaURa during the dry season after peat fires in 2015 showed that microbial SRBM (mixture of N fixing, solubilizing P and cellulolytic microorganisms) from the modified recharge bio-pore system was able to improve the growth of roots, stems, and leaves, nutrient uptake, and increase production and biomass of soybean. In another research in 2018, the use of commercial biofertilizers containing Azotobacter sp and Lactobacillus sp applied to the LaURa peats significantly increased the total N and pH of peats but did not accelerate the decomposition of peat materials. In subsequent study on the exploration of nitrogen fixing microorganisms from the LaURa peats (sapric peats) which amounted to 10375-10500 cells g−1, the total N-fixing microbial population was correlated positively to the pH and EC of peats, and correlated negatively to the contents of peat organic C. Combining the results, the total N2 fixing microbial (NFM) population was significantly affected by organic C and peat EC (total NFM population = -154.052 + 33.126 pH + 607.117 EC, R2 = 51.63%). Results obtained from these studies demonstrated that the functional microbes of peatlands are able to maintain ecological functions of the peats.

Seasonal Water Quality Study of Nche Stream, A Water Body in Imo State, Nigeria

Seasonal variations water quality of Nche stream in carried out using standard methods. The study lasted between April, 2014-March, 2015. Results obtained revealed that pH of the Nche stream varied from 6.41-6.87 in rainy season to 5.50-6.10 in dry season. Mean values for total solid, total dissolved solid, and total suspended solid for rainy season were 208.32 mg/l, 91.81 mg/l, and 116.50 mg/l respectively, while 139.88 mg/l, 72.47 mg/l, and 67.40 mg/l were the respective mean values for total solid, total dissolved solid and total suspended solid in dry season. Bacterial isolates from the stream include Actinomyces sp., Bacillus sp., Staphylococcus sp., Lactobacillus sp, Streptococcus sp., Achromobacter sp., Klebsilla sp., Salmonella sp., Escherichia sp., Acinetobactor sp., Serratia sp., Proteus sp., Enterobacter sp., Vibrio sp., Shigellasp, Flavobacterium sp., Citrobacter sp., Micrococcus sp., and Pseudomonas sp., while saprophytic mould isolates were Penicilum sp., Aspergillus sp., Penicillium sp., Fusarium sp., Cladosporium sp., Rhizopus sp., and Geotricum sp. Levels of cadmium for Nche stream from August to the rest of dry season as observed did not fall within WHO limit. Mean values of THBC, THUB, TCC, SCC, TVB, and TFC observed in the studied stream water were higher in rainy seasons than dry season and high than their respective WHO limits. The ability of cadmium heavy metal to accumulate in the body and the infective nature of the isolated organisms could pose a problem to the locals who consume water from this stream without further purification. There is need to inform the local population on the seasonal pollution status of Nche stream since they heavily depend on this stream as a source of domestic water. This study has evaluated the seasonal water quality of Nche stream, a water body in Imo State, Nigeria.

AMOUNT AND TYPE OF MICROBES ON THE COMMON CARP (Cyprinus carpio) IN JATILUHUR RESERVOIR WEST JAVA

This research was conducted in Microbiology Laboratory of Biology Department, Faculty of Mathematics and Natural Sciences, Padjadjaran University in May 2017. The purpose of the research was to identify the number and types of microbes contained in carp (Cyprinus carpio) in Jatiluhur Reservoir, West Java. The method used in this research was sample survey method or field survey. The water and common carp sampling stations are divided into 3 stations, station 1 is located in Zone 1 (low density), station 2 is located in Zone 3 (medium density), and station 3 is located in Zone 5 (high density). Determination of sampling location water and fish is done based on Data Recapitulation of Density of Karamba Floating Net from the Department of Animal Husbandry and Fisheries of West Java.Water samples and fish mucus were tested using Total Plate Count (TPC) method using different specific media that is Trypticase Soy Agar (TSA), deMann Ragosa Sharpe Agar (MRSA), Kliger Iron Agaar (KIA), Thiosulphate Citrate Bile salts Sucrose Agar (TCBS), and Xylose Lysine Deoxycholate (XLD). Microbes can be known by species because they just grown on specific media that can directly showed the specific characteristics of certain microbial species based on the color of the colony. The parameter observed were microbiological test (TPC). The result of the research showed that there was Bacillus sp, Escherichia coli, Lactobacillus sp, Vibrio cholerae, Vibrio parahemoliticus, Vibrio spp, dan Shigella sp on the water samples and carp mucus. The result of research on water samples and fishery products in Jatiluhur Reservoir showed that water quality in Zone 1, Zone 3, and Zone 5 is still below the threshold of microbial contamination based on SNI 7388: 2009, while fish located in Zone 3 and Zone 5 is contaminated by Escherecia coli and Vibrio cholerae, exceed the threshold of SNI 7388: 2009 on microbial contamination in fresh fish.

Analysis of Fibre Fraction of Palm Oil Frond Fermented with Different Microbes and Soluble Carbohydrates Addition as Ruminant Feeding

This research aimed to analyse the fibre content of palm oil fronds fermentation as a feeding of ruminant for substitution of native grass Fermentation of palm oil frond was done using local microorganisms resources rumen content and Lactobacillus sp. The experimental design used was factorial on complete randomized design 2×3 with 3 replication each treatment. Factor A was the source of inoculums fermentation, where A1=. Lactobacillus sp, A2= local microorganism resources of rumen content, whereas Factor B was the source of soluble carbohydrates where B1 = without a carbohydrates, B2 = rice bran, B3 = tapioca starch. The variables measured were the content of ADF, NDF, cellulose, hemicellulose, lignin, and Silica. The results of this research showed that interaction affect between the source of microorganisms with carbohydrates just on cellulose, no for the other parameters. Factor A were significantly (P < 0.01) on the content of lignin, and fermentation by local microorganism resources of rumen content more lower than Lactobacillus sp. (15.41 Vs 20.71%). The effect (P < 0.05) factor B on ADF, NDF with addition by tapioca starch. This treatment could reduce the content of fibre fraction.

Isolation and Characterization of Lactic Acid Bacteria (Lactobacillus sp) from Sauerkraut with the addition of Cayenne Pepper

Sauerkraut is cabbage that is produced from natural fermentation by bacteria in the presence of 2.5 percent salt. Salt additions limits the activity of gram –negative bacteria, while the growth of lactic acid bacteria will increase. Chili is a vegetable of the genus Capsicum which has high economic value and also contains various compounds that are useful for human health. One method to get good quality cayenne is fermentation. Fermentation is part of biotechnology that uses microorganisms as the main actors in a process. One of the microbes that determines the success of fermentation is lactic acid bacteria. The purpose of this study is isolate LAB from Sauerkraut with the additional cayenne pepper the type of LAB produced microscopically. Research methods, the ingredients used are cabbage and cayenne fermentation (sauerkraut with the additional cayenne pepper), MRSa, 0,9% NaCl, crystal violet paint from biological laboratories UNP. Isolation LAB from Sauerkraut done in with fermentation Sauerkraut and then plant the sauerkraut into the MRSa medium with streak plate methods. The isolates obtained were identified microscopically using a microscope with gram staining method. From the research that has been done, the following result are obtained : Sauerkraut with additional cayenne pepper direcly into MRSa medium and gram staining, there were 12 colonies of gram positive bacteria with bacil cell form, and negative catalase test. We can identify this colonies as Lactobacillius sp .

Characterization and evaluation of antibacterial efficacy of a novel antibiotic-type compound from a probiotic strain Lactobacillus plantarum KJB23 against food-borne pathogens

Probiotic lactobacilli and its molecules to be used as natural antimicrobials to kill food-borne pathogens as well as therapeutic agents for better development of human gut have drawn amazing attention among consumers. Thus the present study was aimed to screen a probiotic Lactobacillus plantarum (Lp) KJB23 strain for the production of bioactive compound and it was purified using cell adsorption-desorption technique followed by chromatographic techniques. The antilisterial activity of purified compound was not decreased during protease treatment, confirming the non-proteinaceous nature. The compound was identified as 3,4,5,8-tetrahydroxyhexahydro-2H-pyrano[2,3-d][1,2]dioxin-2-one and its molecular weight was found to be 222.1490 Da using HRMS-ESI. This compound has not been reported so far from Lactobacillus sp. The notable bactericidal effect of compound may have potential to be used as antimicrobial agent in food industry as evidenced by pore formation on cell membrane of L. monocytogenes together with its non-cytotoxicity effect. According to our results, the effective non-proteinaceous and non-cytotoxic low molecular weight antibiotic-type compound may have valuable attribute in view of their use in efficient food improvement and can be supportive for developing alternate remedial approaches against food-borne and human pathogens.

Production of Virgin Cococnut Oil (VCO) Catalized by Lactobacillus plantarum and Enzymes Contained in Pineapple Fruit Extract

Indonesia is a tropical country with an area of coconut plantation of 3.7 Ha. The fruits of this plant have been used as materials in the production of Virgin Coconut Oil (VCO). Such oil is known to contain higher Medium Chain Fatty Acid (MCFA) or Medium Chain Triglycerides (MCTs) than original frying oil. The MCTs of this oil is very useful to avoid obesity, to reduce total cholesterol as well as to function as anti-microbes. These have been considered as a cheap solution to solve health related problems. The main objective of this research was to produce VCO catalyzed by pure culture of Lactobacillus plantarum isolated from a commercial VCO (Vici’O) combined with enzymes extracted from pineapple fruits. The study was conducted at the laboratory of Microbiology, Faculty of Mathematics and Sciences, Udayana University. The study was started with isolation and identification of Lactobacillus sp. from a commercial VCO. The VCO production method combined the two starters of Lactobacillus isolates (at concentrations of 0 to 10% v/v) and pineapple juice with various volumes (0 to 60 mL). In the first stage of the project, 3 potential Lactobacilli isolates (Sp1, Sp2, and Sp3) were successfully isolated and were confirmed to be Lactobacillus sp. In the main experiment, combination of 10% v/v Lactobacillus starter and 60 mL pineapple juice produced the highest VCO yield (43,17% v/v) with improved organoleptic properties, lower water content, lower saphonification, and lower peroxide than that specified by SNI No 7381: 2008.

The Probiotic Bacterium Isolated from Bekasam (Traditional Fermented Food), Lactobacillus Sp. Induces Activation of Gut Mucosal Immune System in Rat.

BACKGROUND:Bekasam is one of the traditional foods in South Sumatra, Indonesia, a mixture of fermented fish containing Lactic Acid Bacteria (LAB), Lactobacillus sp. Non-commensal bacteria and probiotics can induce intestinal mucosal immune responses.AIM:This pilot study aimed to see the efficacy of Lactobacillus sp. to the immune response of the intestinal mucosa by assessing the levels of IgA in the intestinal fluid and markers of T cell populations, such as CD4 and CD8 in the intestinal mucosa.METHODS:This study was an in vivo experimental study. As many as 30 rats were grouped into 3 treatment groups (doses 107, 108, and 109 CFU/rat/day, for 7 days) and 2 groups of controls (negative control, 10% non-fat milk, and positive control, Lactobacillus casei 108 CFU/rat/day for 7 days). At the end of the treatment, the intestinal mucosa was taken to examine the levels of IgA, CD4 and CD8 using the Enzyme-Linked Immunosorbent Assay (ELISA) method, according to the manuals of each ELISA kit. All displays of research data were presented with means ± SD. T-test was used to assess the significance of differences.RESULTS:Secretion of Ig A increased with the addition of Lactobacillus sp. from bekasam. Administration of Lactobacillus sp. yielded no effect on helper T cell level (CD4 markers), as well as on cytotoxic T cell levels (CD8 markers).CONCLUSION:Lactobacillus sp. probiotic from bekasam improved the intestinal mucosal immune system by increasing the production of Ig A, but exhibited no effect on T lymphocyte cells.

Bacterial Populations in Subgingival Plaque Under Healthy and Diseased Conditions: Genomic Insights into Oral Adaptation Strategies by Lactobacillus sp. Strain DISK7.

Human oral cavity is a complex habitat comprising about 700 microbial species and represents the most complex microbiota after gastrointestinal tract. In fact, oral microbiota directly influences health, metabolism and immune responses of the host. Metagenomic studies based on 16S rDNA profiling has reported the inhabitant bacteria mainly belonging to phyla Firmicutes, Proteobacteria, Actinobacteria, Fusobacteria, Spirochaetes and Bacteroidetes. Therefore, it is essential to isolate these strains and characterize in detail to understand their interaction. We have isolated strains from subgingival plaque from healthy to diseased individuals and the molecular characterization based on 16S rRNA gene sequence analysis showed predominance of Firmicutes, specifically members of the genus Streptococcus. Species of Lactobacillus and Veillonella were also found in significant number, which are considered as secondary colonizers. However, the population of Lactobacillus was decreased in diseased conditions with the increase in opportunistic pathogenic strains pertaining to genera like Campylobacter, Neisseria, Enterobacter, Pseudomonas and Morococcus. Further, we have also made an attempt to gain genomic insights on adaptation features and interactions of an isolate, Lactobacillus sp. strain DISK7 by performing whole genome sequencing and analysis, subsequently biochemical characterization to explore its functional and metabolic properties for the development as probiotic agent.