Acidophilus Supplementation Research Articles

Bacterial Community Influences the Effects of Lactobacillus acidophilus on Lipid Metabolism, Immune Response, and Antioxidant Capacity in Dogs.

Lactobacillus acidophilus (L. acidophilus), the most prevalent probiotic, has demonstrated the ability to improve the relative abundance of intestinal microorganisms and boost immunity. However, the underlying mechanisms of these effects remain unclear. This study evaluated body weight, nutrient apparent digestibility, serum indices, and bacterial communities in Chinese rural dogs from a L. acidophilus supplementation group (Lactobacillus acidophilus, n = 6) and a control group (CON, n = 6). The results indicated that L. acidophilus had no significant impact on the body weight and apparent nutrient digestibility of Chinese rural dogs. In comparison with the CON group, L. acidophilus significantly reduced the levels of cholesterol (CHO) and increased the levels of IgA, IFN-α, and T-AOC. Bacterial diversity indices were significantly reduced in the LAC group compared to the CON groups, and MetaStat analysis demonstrated notable distinctions in 14 bacterial genera between the groups. These bacterial genera exhibited correlations with physiological indices such as CHO, IgA, IFN-α, and T-AOC. In conclusion, L. acidophilus can modulate lipid metabolism, immunity, and antioxidant capacity by regulating the relative abundance of specific bacterial communities, which helps dogs to adapt to today's lifestyle.

Alternative to antibiotic growth promoters: beneficial effects of Saccharomyces cerevisiae and/or Lactobacillus acidophilus supplementation on the growth performance and sustainability of broilers' production.

Although antibiotics growth promoters (AGPs), including zinc-bacitracin (ZnB), can threaten human health due to developing antimicrobial resistance, as well as drug residue in animal and poultry products, ZnB is still widely used, particularly in developing countries, for the sustainability of poultry farming. The present investigation aims to assess the use of Saccharomyces cerevisiae and Lactobacillus acidophilus, with or without a prebiotic (mannooligosaccharide, MOS), as alternatives to ZnB. For this reason, 150 one-day-old chicks were grouped into six groups, designated negative control, LA, SC, ZnB, SA + MOS, and LA + MOS (5 replicates of 5 chicks for each group). Chicks kept in the control group were fed the basal diet. Chickens kept in LA and SC groups received L. acidophilus, S. cerevisiae at a 1 g/kg diet and 2 g/Kg, respectively. Chickens kept in ZnB received ZnB at 0.5 g/kg. Chicks kept in the SC + MOS and LA + MOS were fed a basal diet containing 2 g S. cerevisiae + 1 g MOS/kg or 1 g L. acidophilus + 1 g MOS /kg, respectively. The efficacy was assessed based on the growth performance, carcass traits, meat quality, nutrient digestibility, and blood biochemistry composition during the entire trial 1-36 days of age. Results showed that chicks kept in the SC group had greater BW than the control (p < 0.05). Chicks kept in the SC, LA, SC + MOS, and LA + MOS consumed less feed than the control and Zn-B groups (p < 0.05). Supplementation with S. cerevisiae resulted in a better (p < 0.05) feed conversion rate (FCR) than the control group. Supplementation with L. acidophilus + MOS significantly increased (p < 0.05) the relative liver weight compared to those supplemented with ZnB, S. cerevisiae, and L. acidophilus. In addition, supplementation with ZnB-induced spleen hypertrophy compared to S. cerevisiae and L. acidophilus-supplemented groups (p < 0.05). Plasma, meat, and liver cholesterol, as well as the cholesterol-to-lipid ratio of meat and liver, were significantly decreased (p < 0.05) in both SC and LA groups compared to the control group. Our research indicates that adding 2 g/kg of S. cerevisiae to broiler feed can effectively replace ZnB and enhance productive performance and economic profits, making it a viable and sustainable option for broiler farming.

Lactobacillus acidophilus Supplementation Improves the Innate Immune Response and Disease Resistance of Striped Catfish (Pangasianodon hypophthalmus Sauvage, 1878) Juveniles against Aeromonas hydrophila

A bacterium Lactobacillus acidophilus (LAB) was evaluated for its probiotic potential on the health status of striped catfish (Pangasianodon hypophthalmus, Sauvage, 1878) juveniles. Triplicate groups of striped catfish (21.69 ± 0.18 g) were fed twice daily at 2.5 % of body weight, with 0 (control), 103, 105, 107 and 109 CFU/g LAB diets for 12 weeks. The fish were challenged intraperitoneally with 1×106 CFU mL−1 of Aeromonas hydrophila after 12 weeks of feeding trial and the mortalities were recorded over 21 days. The haematological, immunological parameters and histopathological changes of liver were assessed both in pre- and post-challenged fish groups. Results revealed that feeding the fish with supplemented diets significantly increased (p &lt; 0.05) the survival after challenged with A. hydrophila. Compared to the control diet, a significant improvement in packed cell volume, red blood cell, white blood cell and lymphocyte counts were observed in LAB supplemented diets in the pre-challenged fish groups. After 3-week post challenged, the hematological parameters, immunoglobulin and survival were significantly high in fish fed with LAB supplemented diets at 105 CFU/g and above, while only the lysozyme activity was significantly high after 2-week post-infection. Histopathological result revealed that severe necrosis was found in the liver of striped catfish fed with the control diet after challenged with A. hydrophila which was not seen in the case of LAB supplemented diets. Thus, the results suggest that administration of LAB at 105 CFU/g of diet improves the innate immune response of striped catfish juveniles. HIGHLIGHTS Dietary Lactobacillus acidophilus (LAB) supplementation improved survival of striped catfish after challenge with Aeromonas hydrophila LAB supplementation enhanced the non-specific immunity of juvenile’s striped catfish LAB supplementation improved the histopathology of pre- and post-challenge liver of striped catfish juveniles GRAPHICAL ABSTRACT

Lactobacillus acidophilus Supplementation Restores Gut Epithelial Integrities and Barrier Functions in Non-specific Diarrhea

Objectives: The study was aimed to evaluate the roles of Lactobacillus acidophilus supplementation in maintaining intestinal epithelial integrities, tight junction proteins, and adhesion molecules in non-specific diarrhea.&#x0D; Methods: In this study, we used the pre-weaned BL/6 pups (3 weeks of age, same litters) as a model animal. We supplied the non-sterilized and poor-quality water to experimental pups (n=7) to develop non-specific diarrheic symptoms. Then diarrheic pups were supplemented with Lactobacillus acidophilus (LA) for three consecutive weeks. The control group (n=5) was supplied with sterilized water and no LA. The sampling and analysis were performed on day 0, day 7, day 14, and day 21. The expressions of pro-inflammatory cytokines (IL-6, TNF-a) and tight junction proteins (TJPs) of gut mucosa were determined using qRT-PCR. And the serum cytokines level was screened through sandwich ELISA.&#x0D; Results: The intestinal cytoskeletal integrity becomes disrupted and characterized by lower ZO-1, Occludin, Claudin-1, Claudin-5, and JAM mRNA expressions upon real-time qRT-PCR. However, Claudin-4 was found to be not affected and illustrated with a higher expression like control pups. Interestingly, supplementing Lactobacillus acidophilus was found to maintain gut integrity and effectively reduce diarrheic symptoms. Like the control pups, the Lactobacillus acidophilus supplemented pups exhibited a higher expression of gut epithelial TJPs and adhesion molecules. Moreover, the diseased pups produced significantly increased IL-6, and TNF-α production in blood serum, compared to control BL/6 pups. &#x0D; Conclusion: We concluded that L. acidophilus supplementation might orchestrate the equilibrium of gut health and immunity against non-specific diarrhea.

Lactobacillus acidophilus Supplementation Exerts a Synergistic Effect on Tacrolimus Efficacy by Modulating Th17/Treg Balance in Lupus-Prone Mice via the SIGNR3 Pathway.

ObjectiveTacrolimus (Tac) is an immunosuppressant used in the treatment of systemic lupus erythematosus (SLE); however, it induces T cell subset imbalances by reducing regulatory T (Treg) cells. Lactobacillus acidophilus (LA) is reported to have therapeutic efficacy in immune-mediated diseases via T cell regulation.MethodsThis study investigated whether a combination therapy of LA and Tac improves the therapeutic efficacy of Tac by modulating T cell subset populations in an animal model of SLE. Eight-week-old MRL/lpr mice were orally administered with 5 mg/kg of Tac and/or 50 mg/kg of LA daily for 8 weeks. Cecal microbiota compositions, serum autoantibodies levels, the degree of proteinuria, histological changes in the kidney, and populations of various T cell subsets in the spleen were analyzed.ResultsMice presented with significant gut dysbiosis, which were subsequently recovered by the combination treatment of Tac and LA. Double negative T cells in the peripheral blood and spleens of MRL/lpr mice were significantly decreased by the combination therapy. The combination treatment reduced serum levels of anti-dsDNA antibodies and Immunoglobulin G2a, and renal pathology scores were also markedly alleviated. The combination therapy induced Treg cells and decreased T helper 17 (Th17) cells both in vitro and in vivo. In vitro treatment with LA induced the production of indoleamine-2,3-dioxygenase, programmed death-ligand 1, and interleukin-10 via the specific intracellular adhesion molecule-3 grabbing non-integrin homolog-related 3 receptor signals.ConclusionThe present findings indicate that LA augments the therapeutic effect of Tac and modulates Th17/Treg balance in a murine model of SLE.

Effects of Lactobacillus acidophilus on the growth performance, immune response, and intestinal barrier function of broiler chickens challenged with Escherichia coli O157

We studied the effects of Lactobacillus acidophilus (L. acidophilus) on the growth performance, intestinal morphology, barrier function, and immune response of broilers challenged with Escherichia coli O157 (E. Coli). A total of 360 1-day-old Cobb male broilers were tested in a 3 × 2 factorial arrangement with 3 dietary L. acidophilus levels (0, 5 × 108 CFU/kg, and 10 × 108 CFU/kg of diet) and 2 disease challenge treatments (control or E. coli challenged). Results showed that E. coli challenge decreased the ADG, ADFI, and BW of broilers from 15 to 21 d (P < 0.05), increased the jejunum intestinal wall thickness, and significantly increased the mortality rate. E. coli challenge significantly (P < 0.05) decreased the serum IgA and IgM contents and peripheral blood CD3+ T cell counts (P < 0.05), increased the serum CRP, DAO, and LPS levels at 21 d; upregulated the mRNA expression of iNOS, IL-8, IL-1β in the jejunum and iNOS in the spleen, and downregulated the occludin and ZO-1 mRNA expression in the ileum at 21 d compared with uninfected birds (P < 0.05). Dietary L. acidophilus supplementation consistently showed higher BW, ADG, ADFI, and jejunum and ileum V:C ratio at 14 d and 21 d in the presence and absence of E. coli challenge (P < 0.05). L. acidophilus supplementation reduced the mortality rate caused by E. coli challenge (P < 0.05), decreased the serum CRP, DAO, and LPS levels at 14 d and 21 d; upregulated the mRNA expression of occludin and ZO-1 in the jejunum and ileum, and downregulated the mRNA expression of iNOS, IL-8, and IL-1β in the jejunum in E. coli challenged birds at 21 d (P < 0.05). Dietary supplementation with L. acidophilus can improve the growth performance, intestinal health, and survival of broilers challenged with E. coli.

The effect Moringa oleifera leaf extract and Lactobacillus acidophilus supplementation on crude protein and crude fat retention in Tambaqui, Colossoma macropomum

Feed contributes to nearly 70% of the total production cost in aquaculture industries. This study aims to determine the effect of Moringa leaf extract and probiotics (Lactobacillus acidophilus) supplementation in commercial feed on protein content and crude fat content of Tambaqui fish, Colossoma macropomum. This study used a factorial completely randomized design consisting of 3 × 3 treatments with 3 treatments. Fish were cultured for 30 days each. The results showed that the administration of Moringa leaf extract at different doses had a significant effect on crude protein content, but did not have a significant effect on crude fat content. Other results showed that there was a significant effect of probiotic supplementation crude fat content, but had a significant effect on the protein content of Tambaqui. The best interaction treatment was A2B0 treatment with crude protein value 19.93% and A0B1 treatment with crude fat value 1.49%.

Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis.

ABSTRACTHuman toxocariasis consists of chronic tissue parasitosis that is difficult to treat and control. This study aimed to evaluate the action of the probiotic Lactobacillus acidophilus ATCC 4356 on larvae of Toxocara canis and the effect of IFN-γ cytokine on parasite-host in vivo (1.109 CFU) and in vitro (1.106, 1.107, 1.108, 1.109 CFU) interactions. Four groups of six BALB/c mice were formed: G1 - L. acidophilus supplementation and T. canis infection; G2 - T. canis infection; G3 - L. acidophilus supplementation; and G4 - PBS administration. Mice were intragastrically suplemented with probiotics for 15 days before inoculation and 48 h after inoculation with 100 T. canis eggs. The inoculation of T. canis was also perfomed intragastrically. The recovery of larvae took place through digestion of liver and lung tissues; the evaluation of IFN-γ gene transcription in leukocytes was performed by qPCR. The in vitro test consisted of incubating the probiotic with T. canis larvae. The supplementation of probiotics produced a reduction of 57.7% (p = 0.025) in the intensity of infection of T. canis larvae in mice, whereas in the in vitro test, there was no larvicidal effect. In addition, a decrease in the IFN-γ gene transcription was observed in both, T. canis-infected and uninfected mice, regardless of whether or not they received supplementation. The probiotic L. acidophilus ATCC 4356 reduced T. canis infection intensity in mice, however, the probiotic did not have a direct effect on larvae, demonstrating the need of interaction with the host for the beneficial effect of the probiotic to occur. Yet, the proinflammatory cytokine IFN-γ did not apparently contributed to the observed beneficial effect of probiotics.

Dietary Supplementation of Probiotic Lactobacillus acidophilus Modulates Cholesterol Levels, Immune Response, and Productive Performance of Laying Hens.

Simple SummaryChicken eggs provide a considerable source of high-quality nutrients for human food and health. However, egg consumption may harm some people for its high contents of cholesterol and association with cardiovascular disease risk. Therefore, we investigated the possible effect of using Lactobacillus acidophilus (LA) as a probiotic additive in the laying hens’ diets on lowering egg-yolk cholesterol and also evaluated the immune responses and the productive performance of laying hens. The obtained results display the ability of LA, when supplemented in hen diets, to decrease the plasma triglycerides and cholesterol levels and the liver and egg yolk cholesterols in the hen. The beneficial effects of LA were also explored on some important humoral and cell-mediated immune responses in the laying hens. These positive effects led to an improvement in the productive performance of laying hens. Therefore, dietary LA supplementation could be recommended as a nutritional strategy for commercial lower-cholesterol egg production in addition to positive impacts on the performance and health of laying hens.This study examines the effect of dietary supplementation with Lactobacillus acidophilus (LA) on the cholesterol levels, immune response, and productive performance of laying hens. A total of 216, 40-week-old, commercial Hy-Line brown chicken layers were randomly assigned into four treatment groups (18 birds × three replicates per group) and fed diet supplemented with 0 (control), 1 × 109, 21 × 109, and 31 × 109 colony forming units (CFUs) of Lactobacillus acidophilus (LA) per kg of feed for six consecutive weeks. Results show that plasma triglycerides, low-density lipoprotein (LDL) and total cholesterols became lesser, while high-density lipoprotein (HDL) cholesterol became higher in LA-supplemented groups compared to the control. In addition, a significant reduction occurred in the liver and egg yolk cholesterol by LA supplementation. Moreover, the immunological parameters including antibody titer against sheep red blood cells (SRBCs), phytohemagglutinin (PHA)-wattle swelling test, and T- & B-lymphocyte proliferation were enhanced in laying hens supplemented with LA compared to the control hens. While the heterophil to lymphocyte (H/L) ratio decreased with LA supplementation, indicating low stress conditions in the treated hens. These positive effects for LA were further reflected on the productive performance of laying hens and improved egg production, egg weight, egg mass, and feed efficiency. Our findings indicate that LA probiotic could be recommended in laying hens’ diets for lowering egg yolk cholesterol with positive impacts on health and performance.

Effects of dietary extruded linseed and Lactobacillus acidophilus on growth performance, carcass traits, plasma lipoprotein response, and caecal bacterial populations in broiler chicks

The study aimed to assess the effects of dietary extruded linseed (ELS) and Lactobacillus acidophilus (LA) on performance, carcass traits, immune organ weights, plasma lipoprotein response, and caecal bacteria populations in broiler chickens. A total of 648 one-day-old chicks were divided into six groups in a 3 × 2 factorial arrangement consisting of three ELS levels (0, 6 and 12%) without or with probiotic L. acidophilus (0 and 20 g ton−1 feed). Each group had six replicates (18 birds/pen). Results showed no significant effects of ELS level nor LA supplementation on growth performance (body weight gain, feed intake, and feed conversion) of broilers during the overall period (1–42 days). The production efficiency factor increases (p = .045) with the LA addition. There were no effects of ELS level nor probiotic addition on carcase traits and immune organ weights, except for decreases in abdominal fat percentage (p = .027, respectively p = .035). The dietary ELS level significantly correlated with a decrease in the plasma total cholesterol (TC; p = .003), triglycerides, and very-low-density lipoprotein concentrations (p = .020), while the LA addition significantly correlated with a decrease (p = .015) in the plasma TC. The LA addition lowers the caecal pH (p = .007), Staphylococcus spp. and E. coli counts (p < .0001), and increase the caecal Lactobacillus spp. and lactobacilli: E. coli ratio (p < .0001). In conclusion, the use of ELS up to 12% supplemented with L. acidophilus in broiler chicks diet had a positive effect on health status, decreasing the abdominal fat deposition, plasma lipids and the caecal pathogen bacteria E. coli. Highlights Extruded linseed up to 12% in broiler chicks’ diets had no significant effects on growth performance, carcass traits or immune organs size and improve the abdominal fat deposition and plasma lipids response due to the decreased dietary n − 6: n − 3 PUFA ratio. Extruded linseed up to 12% supplemented with L. acidophilus in broiler chicks diet had a positive effect on health status improving production efficiency factor and decreasing abdominal fat deposition, the plasma lipids and the caeca pathogen bacteria E. coli.

Dietary supplementation with Lactobacillus acidophilus enhanced the growth, gut morphometry, antioxidant capacity, and the immune response in juveniles of the common carp, Cyprinus carpio.

This study investigated the effect of Lactobacillus acidophilus-fortified diets on growth performance, antioxidant profiles, and immunity-related gene expressions of common carp, Cyprinus carpio juveniles. Four isonitrogenous diets containing 0, 102, 104, or 106cfu/mL/kg L. acidophilus were fed to 240 Cyprinus carpio juveniles (mean = 21.34 ± 1.85g), allotted to 12 rectangular tanks in a completely randomized designed at 3% body weight for 56days. Growth performance and nutrient utilization were evaluated using standard procedures. Intestinal villi were measured, antioxidant profiles were evaluated from blood sera, and immunity-related gene expressions were evaluated. The results revealed that fish fed dietary 106 had significantly higher weight gain, SGR, feed intake, and lower FCR. Also, villi length, width, and areas of absorption were significantly improved in a dose-dependent manner. Furthermore, innate immune profiles, superoxide dismutase, catalase, respiratory bust activity as well as transforming growth factor beta (TGFβ), interleukin 8 (IL-8), and tumor necrosis factor alpha (TNF-alpha) were significantly stimulated. This study evoked that Lactobacillus acidophilus supplementation enhanced better growth performance, improved antioxidant profiles, and modulated expression of immune-related genes of common carp, Cyprinus carpio, than the fish fed control diet. Results show that fish fed fortified diets had better growth performance, improved antioxidant profiles, and modulated expression of immune-related genes.

Lactobacillus rhamnosus GG supplementation modulates the gut microbiota to promote butyrate production, protecting against deoxynivalenol exposure in nude mice

Deoxynivalenol (DON) is the most common mycotoxin in grains, and DON exposure causes gastrointestinal inflammation and systemic immunosuppression. The immunosuppression caused by DON has raised serious concerns about whether it is safe to use probiotics in immunocompromised hosts. Gut microbiota remodeling by Lactobacillus is a potential effective strategy to prevent DON exposure. The athymic nude mice were chose as the model of immunocompromised animals. We tested the effect of the probiotic Lactobacillus rhamnosus GG (LGG) or Lactobacillus acidophilus (LA) supplementation on host protection against DON exposure and the underlying mechanisms in nude mice. DON exposure induced endoplasmic reticulum (ER) stress and impaired intestinal barrier function and microbiota, which were relieved by LGG supplementation but not LA supplementation. LGG supplementation significantly enhanced the intestinal barrier function, increased the body weight and the survival rate in nude mice that exposed to DON for two weeks. Furthermore, LGG supplementation modulated the gut microbiota by increasing the abundance of Bacteroidetes and the levels of the butyrate-producing genes But and Buk to promote butyrate production. Butyrate inhibited the IRE1α/XBP1 signaling pathway to reduce DON-induced intestine injury. In conclusion, LGG supplementation modulated the gut microbiota to promote butyrate production, protecting against DON exposure in nude mice. Both LGG and butyrate show promise for use in protecting against DON exposure.

Effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier function, and the expression of NOD-like receptors in weaned piglets.

Lactobacillus supplementation is beneficial to the barrier function of the intestinal physical barrier in piglets. However, the mechanisms underlying this beneficial function remain largely unknown. Here, we investigated the effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier functioning, and NOD-like receptors (NLRs) expression in weaned piglets. Sixteen weaned piglets were randomly allocated to two groups. The control group received a corn-soybean basal diet, while the treatment group received the same diet adding 0.1% L. acidophilus, for 14 days. As a result, dietary L. acidophilus supplementation was found to increase the average daily gain (ADG) (P < 0.05), reduced serum diamine oxidase (DAO) activity (P < 0.05), increased the mRNA expression and protein abundance of occludin in the jejunum and ileum (P < 0.01), reduced the mRNA levels of NOD1 (P < 0.01), receptor interacting serine/threonine kinase 2 (RIPK2) (P < 0.05), nuclear factor κB (NF-κB) (P < 0.01), NLR family pyrin domain containing 3 (NLRP3) (P < 0.01), caspase-1 (P < 0.01), interleukin 1β (IL-1β) (P < 0.05) and IL-18 (P < 0.01) in the jejunum tissues of the weaned pigs. The expression of NLRP3 (P < 0.05), caspase-1 (P < 0.01), IL-1β (P < 0.05) and IL-18 (P < 0.05) was also reduced in the ileum tissues of the weaned pigs. These results showed that L. acidophilus supplementation improves the growth performance, enhances the intestinal physical barrier function, and inhibits the expression of NOD1 and NLRP3 signaling-pathway-related genes in jejunum and ileum tissues. They also suggest that L. acidophilus enhances the intestinal physical barrier functioning by inhibiting IL-1β and IL-18 pro-inflammatory cytokines via the NOD1/NLRP3 signaling pathway in weaned piglets.

Effects of Lactobacillus acidophilus on the growth performance and intestinal health of broilers challenged with Clostridium perfringens

BackgroundClostridium perfringens is the main etiological agent of necrotic enteritis. Lactobacilli show beneficial effects on intestinal health in infectious disease, but the protective functions of lactobacilli in C. perfringens-infected chickens are scarcely described. This study examined the effects of Lactobacillus acidophilus (L. acidophilus) on the growth performance and intestinal health of broiler chickens challenged with Clostridium perfringens (C. perfringens) over a 28-day period. Using a 2 × 2 factorial arrangement of treatments, a total of 308 1-day-old male Arbor Acres broiler chicks were included to investigate the effects of Lactobacillus acidophilus (L. acidophilus) on the growth performance and intestinal health of broiler chickens challenged with Clostridium perfringens (C. perfringens) during a 28-day trial.ResultsDuring infection (d 14–21), C. perfringens challenge decreased the average daily gain (P < 0.05), and increased feed conversion ratio and the mortality rate (P < 0.05). However, dietary supplementation with L. acidophilus increased the body weight of C. perfringens-infected broilers on d 21 (P < 0.05), and tended to decrease the mortality (P = 0.061). C. perfringens challenge decreased the villus height (P < 0.05), the ratio of villus height to crypt depth (P < 0.05) and OCLN (occludin) mRNA expression (P < 0.05), and increased the pro-inflammatory cytokine expression in the spleen and jejunum, the intestinal populations of C. perfringens and Escherichia (P < 0.05), and the serum content of endotoxin (P < 0.05), regardless of L. acidophilus supplementation. In contrast, dietary L. acidophilus reducedthe intestinal lesion score of challenged broilers (P < 0.05), the mRNA expression of pro-inflammatory cytokines, ileal populations of Escherichia and serum endotoxin content (P < 0.05), but increased the intestinal Lactobacillus populations (P < 0.05), irrespective of C. perfringens challenge.ConclusionDietary addition of L. acidophilus could improve the intestinal health and reduce the mortality of broilers suffering from necrotic enteritis.

Effects of mannan-oligosaccharides and Lactobacillus acidophilus supplementation on growth performance, nutrient utilization and faecal characteristics in Murrah buffalo calves.

A study of 120days was undertaken to ascertain the effect of mannan-oligosaccharides (MOS) and Lactobacillusacidophilus supplementation on growth performance, nutrient utilization and faecal characteristics in Murrah buffalo calves. Twenty Murrah buffalo calves of 5-7 days old and 31±2.0kg of body weight (BW) were randomly assigned into four groups. Group I served as the control (CON) in which only basal diet (concentrate mixture and green fodder) was provided, without any supplementation. Mannan-oligosaccharides at 4g/calf/day were supplemented as prebiotic to Group II (PRE), whereas Group III (PRO) received Lactobacillusacidophilus in the form of fermented milk as probiotic at 200ml/calf/day having 108 CFU/ml and Group IV (SYN) was supplemented with both MOS and Lactobacillusacidophilus as synbiotic at similar dose. Final BW (kg), dry matter intake, average daily gain, feed conversion efficiency and structural growth measurements were improved (p<.05) in the treatment groups compared to control. Digestibility of neutral detergent fibre was higher (p<.05) in SYN followed by PRE and PRO than control. The faecal lactobacilli and bifidobacterium population was higher (p<.05) in all the supplemented groups with a concomitant reduction in faecal coliform count as compared to control. Faecal ammonia, lactate and pH were also altered favourably (p<.05) in all the supplemented groups as compared to CON. The faecal volatile fatty acids were higher (p<.05) in PRE, PRO and SYN group than CON. The incorporation of MOS and Lactobacillusacidophilus in diet either individually or in combination as synbiotic has the potential to improve the performance and faecal characteristics in Murrah buffalo calves; however, the observed responses among the treatment groups were more evident in the synbiotic fed group compared to individual supplementation of MOS and Lactobacillusacidophilus.

Effects of Lactobacillus acidophilus supplementation on the growth, enzyme activities, and mRNA expression of disease-resistance related enzymes of juvenile Litopenaeus vannamei

PDF HTML阅读 XML下载 导出引用 引用提醒 添加嗜酸乳杆菌对凡纳滨对虾生长、酶活性及相关酶mRNA表达的影响 DOI: 作者: 作者单位: 1. 广东海洋大学 水产动物营养与饲料实验室, 广东 湛江 524088;2. 农业农村部南方水产与畜禽饲料重点实验室, 广东 湛江 524074 作者简介: 李军亮(1991-),男,硕士研究生,研究方向为水产动物营养与饲料.E-mail:junliangli132@163.com 通讯作者: 中图分类号: S968 基金项目: 广东省科技厅社会发展领域科技计划项目（2013B021100017）；农业部南方水产与畜禽饲料重点实验室项目. Effects of Lactobacillus acidophilus supplementation on the growth, enzyme activities, and mRNA expression of disease-resistance related enzymes of juvenile Litopenaeus vannamei Author: Affiliation: 1. Laboratory of Aquatic Animal Nutrition and Feed, Guangdong Ocean University, Zhanjiang 524088, China;2. Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agri-culture and Rural Affairs, Zhanjiang 524074, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:本实验旨在研究饲料中添加嗜酸乳杆菌（）幼虾生长、非特异性免疫酶活性、抗病力和相关酶mRNA表达的影响。选取840尾初始均重为（0.58±0.01）g的凡纳滨对虾幼虾为研究对象，分为7个处理，每个处理3个重复。投喂添加不同比例（0%，0.1%，0.2%，0.4%，0.6%，0.8%，1.0%）嗜酸乳杆菌的饲料，养殖期8周。结果显示：添加不同比例嗜酸乳杆菌对凡纳滨对虾的成活率无显著影响（<0.05），在0.4%组达到最大值；当添加0.2%时，饲料系数最低，其他各组显著低于对照组（<0.05），而蛋白质效率的变化规律则与饲料系数相反。血清酚氧化物酶（phenoloxidase，PO）、碱性磷酸酶（alkaline phosphatase，AKP）和酸性磷酸酶（acid phosphatase，ACP）活性随添加量的增加先升高后降低，分别为0.4%、0.1%和0.2%时，达到最大值。过氧化氢酶（catalase，CAT）、溶菌酶（lysozyme，LZM）和超氧化物歧化酶（superoxide dismutase，SOD）活性，实验组均显著高于对照组（<0.05）。哈维弧菌（<0.05）。以增重率（weight gain rate，WGR）为判断依据，根据折线模型得出，饲料中添加0.23%嗜酸乳杆菌可显著促进凡纳滨对虾生长，并提高非特异性免疫酶活性。 Abstract:An 8-week feeding trial was carried out to investigate the effects of supplementation on the growth performance, nonspecific immunity-related enzyme activities, and disease resistance-related enzyme mRNA expression in juvenile , with initial average weight of 0.58±0.01 g, was randomly assigned to 7 groups in triplicates, with 40 individuals per replicate. Seven isonitrogenous and isolipid diets were supplemented with 0%, 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, and 1.0% in a basal diet, respectively. Following results were observed:nonsignificant difference was found in survival among all the groups (>0.05). Weight gain rate (WGR) and specific growth rate (SGR) increased gradually as dietary supplementation increased up to 0.4%, and then showed decline (<0.05). The minimum feed conversion rate (FCR) was observed on feed supplementation with 0.2% , which was significantly lower than that in the other groups (<0.05), while protein efficiency rate (PER) showed the opposite trend. Serum phenoloxidase (PO), alkaline phosphatase (AKP), and acid phosphatase (ACP) activities increased gradually with increasing dietary levels, and then gradually declined. The maximum values of PO, AKP, and ACP were found in the 0.4%, 0.1%, and 0.2% supplementation groups, respectively (<0.05). Activities of serum catalase (CAT), lysozyme (LSZ), and superoxide dismutase (SOD) of all treatment groups were significantly higher than that of the control group (0%) (<0.05). Gene expression levels of CAT and LZM in all treatment groups were significantly up-regulated when compared with the control group (<0.05). At the end of the feeding trial, the shrimps were challenged by for 96 h. Post-challenge survival rate increased with increasing dietary <0.05). Based on the broken-line regression analysis of WGR, when feed was supplemented with 0.23% of Litopenaeus vannamei were found to significantly improve. 参考文献 相似文献 引证文献

Effects of Lactobacillus acidophilus on gut microbiota composition in broilers challenged with Clostridium perfringens

This study shows the effects of dietary supplementation with Lactobacillus acidophilus on the gut microbiota of broiler chickens challenged with Clostridium perfringens infection during a 21-day period according to pyrosequencing of the 16S ribosomal RNA gene. In a 2 × 2 factorial arrangement of treatments, 308 1-day-old male Arbor Acres broiler chicks were analyzed for the effects of the probiotic (groups without or with L. acidophilus supplementation), pathogen challenge (groups without or with C. perfringens), and the effects of interaction. The infection decreased the number of Observed species, Chao1, and ACE of ileal microbiota and increased Chao1 of cecal microbiota of broilers, whereas L. acidophilus supplementation decreased the Shannon index of the ileal microbiota. Shannon index and Simpson indices were lower in the ileal microbiota than in the cecal microbiota. In the ileal microbiota, the control group had higher relative abundance of Lachnospiraceae and Ruminococcaceae in comparison with the other groups; however, the relative abundance of Gammaproteobacteria was significantly higher in the challenge group than in the other groups. C. perfringens infection tended to increase lactate concentration and decreasedconcentrations of formate, acetate and propionate in the ileum; decreased isobutyrate concentration; and tended to decrease isovalerate concentration in the cecum. Besides, L. acidophilus supplementation increased the concentration of lactate and butyrate and decreased concentrations of formate and propionate in the ileum, and increased concentrations of lactate and valerate in the cecum. In conclusion, C. perfringens infection and/or dietary supplementation with L. acidophilus modulated the relative abundance of some bacteria taxa, and the L. acidophilus supplementation helped to restore the microbial community disrupted by C. perfringens infection.

Effects of Lactobacillus acidophilus and zinc oxide on the growth performance, jejunal morphology and immune function of weaned piglet following an Escherichia coli K88 challenge

This study evaluated the effects of Lactobacillus acidophilus (LA) and zinc oxide (ZnO) supplementation on the growth performance, jejunal morphology and immune function of weaned piglets challenged with Escherichia coli K88. A total of 96 weaned piglets were randomly allocated to four treatments in a 28-day experiment with a 2 × 2 factorial experiment involving two levels of L. acidophilus (0 and 5 × 1010 CFU/kg feed), and two levels of ZnO (0 or 3000 mg/kg feed). One piglet per pen was chosen and orally challenged with Escherichia coli K88 on day 28 and slaughtered at 6 hours post-infection. The results showed that LA supplementation increased the average daily gain from day 0 to day 28 (p = .025). Piglets fed with the diets containing LA or ZnO showed lower feed conversion ratio than the unsupplemented group (p < .05). In addition, LA supplementation increased villus height (VH), villus width and the ratio of VH and crypt depth (CD) compared to the unsupplemented group (p < .001). Administration of ZnO increased VW (p = .001) and VH:CD ratio (p = .039). Moreover, dietary LA and ZnO upregulated the mucin 2 and β-defensin 3 expression in the jejunum (p < .001), and LA supplementation increased immunoglobulin A concentration in the jejunal tissue (p = .019). In conclusion, the administration of LA or ZnO was effective in improving growth performance of weaned piglets, and both supplementations may benefit the jejunal development and immune function of piglets following E. coli K88 challenge.

Effects of Lactobacillus acidophilus supplementation for improving in vitro rumen fermentation characteristics of cereal straws

We explored and compared the effects of four doses (0, 0.25 × 107, 0.50 × 107, and 0.75 × 107 cfu/mL) of Lactobacillus acidophilus supplementation on ruminal fermentation characteristics of maize stover and rice straw. The maximum gas production (Vf) for maize stover and the rate of gas production during early incubation (FRD0) for rice straw were quadratic (p < .05) and linear (p < .01), respectively, and increased when supplemented with L. acidophilus. The L. acidophilus supplementation increased ruminal NH3-N concentration for maize stover (p < .01) and rice straw (p < .05), respectively. The supplementation level of L. acidophilus did not affect the production of volatile fatty acids (VFAs) except for valeric acid, nor did it affect CH4 production, IVDMD, IVNDFD, ruminal pH value, total volatile fatty acids (TVFAs) or the ratio of acetic to propionic acid (A:P). As a fermentation substrate, when compared to rice straw, maize stover increased in vitro gas production parameters (e.g. Vf, k, FRD0 and t0.5), IVDMD, IVNDFD, ruminal NH3-N concentration and TVFAs, whereas there was no (p > .05) difference in CH4 production, ruminal pH value or A:P between the two substrates. The results indicate that L. acidophilus increase in vitro gas production and ruminal NH3-N concentration when using either fermentation substrate, with the greatest increase observed at a dosage of 0.75 × 107 cfu/mL. Additionally, maize stover is superior to rice straw as a fermentation substrate or forage for ruminants. The present positive in vitro results should be tested using in vivo experiments in future.

Effects of Lactobacillus acidophilus supplementation on growth performance, nutrient digestibility, fecal microbial and noxious gas emission in weaning pigs.

Antibiotics used as growth promoters in livestock have been banned in the European Union since 2006. Antibiotics alternatives have focused on probiotics, such as Lactobacillus acidophilus. The concentration of L. acidophilus is considered crucial for obtaining the desired effects. However, limited studies have been conducted to test the dose-dependent effects of L. acidophilus. Therefore, the present study aimed to test the dose-dependent effects of L. acidophilus on growth performance, nutrient digestibility, fecal microbial flora and fecal noxious gas emission in weaning pigs. Lactobacillus acidophilus supplementation increased (P < 0.05) average daily gain, average daily feed intake, apparent nutrient digestibility of dry matter, nitrogen and gross energy, and Lactobacillus counts compared to the basal diet treatment, and a linear effect (P < 0.05) was observed on those criteria. Escherichia coli counts and NH3 emission were decreased (P < 0.05) by L. acidophilus supplementation, and a linear effect (P < 0.05) was observed on E. coli counts. These results suggest that L. acidophilus could be used as an antibiotic alternative by improving growth performance, nutrient digestibility and gut balance (i.e. increased Lactobacillus counts and decreased E. coli counts), and decreasing NH3 emission, of weaning pigs. © 2016 Society of Chemical Industry.