As we know, the medium density fiberboard prepared from natural sticky matter which is generated from the lignin activated through laccase-mediator system has been an effective solution to the problem of free formaldehyde in forestry indus- try. Therefore, based on the premier experiments, this paper has studied the carbon reservation in medium density fiberboard through artificial accelerated aging, and drew the conclusion that the carbon reservation in medium density fiberboard prepared from the wood fiber which is activated through laccase-mediator system is relatively higher than any other board. Further- more, when the variation during artificial accelerated aging is relatively small, the variation of chromatic value bears evident otherness with control samples. This is because laccase-mediator system has modified some chromophore and auxochrome. Meanwhile, the study found that the lower the carbon reservation, the smaller the initial contact angle, the higher the wettabil- ity, and the more perishable the board will be. Nevertheless, the initial contact angle of medium density fiberboard prepared with laccase-mediator system varies little, and begins to stabilize a while after.