Lab-scale Upflow Anaerobic Sludge Bed Research Articles

Dynamic anaerobic membrane bioreactor coupled with sulfate reduction (SrDMBR) for saline wastewater treatment

This study investigated organic removal performance, characteristics of the membrane dynamics, membrane fouling and the effects of biological sulfate reduction during high-salinity (1.0%) and high-sulfate (150 mgSO42--S/L) wastewater treatment using a laboratory-scale upflow anaerobic sludge bed reactor integrated with cross-flow dynamic membrane modules. Throughout the operational period, dynamic membrane was formed rapidly (within 5–10 min) following each backwashing cycle (21–16 days), and the permeate turbidity of <5–7 NTU was achieved with relatively high specific organic conversion (70–100 gTOC/kgVSS·d) and specific sulfate reduction (50–70 gSO42--S/kgVSS·d) rates. The sulfide from sulfate reduction can be reused for downstream autotrophic denitrification. 16S rRNA gene amplicon sequencing revealed that the microbial communities enriched in the sludge were different than those accumulated on the dynamic layer. Overall, this study demonstrates that the anaerobic dynamic membrane bioreactor coupled with sulfate reduction (SrDMBR) shows promising applicability in saline wastewater treatment.

Magnetite enhances anaerobic digestion of high salinity organic wastewater

Biological treatment of high salinity organic wastewater is a significant challenge because many microorganisms involved in the anaerobic digestion process cannot survive high osmotic pressures. In order to alleviate some of the stresses associated with the treatment of high salinity wastewater, two lab-scale up-flow anaerobic sludge bed reactors with or without magnetite (100 g/L) were used to treat high salinity organic wastewater. This study showed that the bioreactor amended with magnetite had higher chemical oxygen demand removal efficiencies (90.2% ± 0.54% vs 73.1% ± 1.9%) and methane production rates (4082 ± 334 ml (standard temperature and atmospheric pressure, STP)/d vs 2640 ± 120 ml (STP)/d) than the non-amended control reactor. In addition, the consumption of volatile fatty acids (20.9 ± 3.4 mM vs 61.7 ± 2.0 mM) was accelerated. Microbial community analysis revealed that the addition of magnetite caused the enrichment of many bacterial genera known to form robust biofilms (i.e. Pseudomonas) that are also capable of extracellular electron transfer and methanogens from the genus Methanosarcina which have been shown to participate in direct interspecies electron transfer. These results show that magnetite addition could enhance the performance of anaerobic digesters treating high salinity wastewater.

Influences of Temperature and Substrate Particle Content on Granular Sludge Bed Anaerobic Digestion

Influences of temperature (25–35 °C) and substrate particulate content (3.0–9.4 g total suspended solids (TSS)/L) on granular sludge bed anaerobic digestion (AD) were analyzed in lab-scale reactors using manure as a substrate and through modeling. Two particle levels were tested using raw (RF) and centrifuged (CF) swine manure slurries, fed into a 1.3-L lab-scale up-flow anaerobic sludge bed reactor (UASB) at temperatures of 25 °C and 35 °C. Biogas production increased with temperature in both high- and low-particle-content substrates; however, the temperature effect was stronger on high-particle-content substrate. RF and CF produced a comparable amount of biogas at 25 °C, suggesting that biogas at this temperature came mainly from the digestion of small particles and soluble components present in similar quantities in both substrates. At 35 °C, RF showed significantly higher biogas production than CF, which was attributed to increased (temperature-dependent) disintegration of larger solid particulates. Anaerobic Digestion Model No.1 (ADM1) based modeling was carried out by separating particulates into fast and slow disintegrating fractions and introducing temperature-dependent disintegration constants. Simulations gave a better fit for the experimental data than the conventional ADM1 model.

Competitive dynamics of anaerobes during long-term biological sulfate reduction process in a UASB reactor

To reveal the long-term competitive dynamics of anaerobes in anaerobic bioreactors with sulfate reduction, a comprehensive structured mathematical model was designed for an extension of the Anaerobic Digestion Model No. 1 (ADM1). Sulfate reduction bacteria (SRB) were categorized into acetogenic-likewise SRB (ASRB) and methanogenic-likewise SRB (MSRB). Experimental data from 329 days of continuous operation of a laboratory-scale upflow anaerobic sludge bed (UASB) reactor was used for model calibration and validation. Results show that the model has a good agreement with experimental data and that three stages including the MPA dominant, stalemate and SRB dominant stages were clearly appeared throughout the whole competition period. The model was capable of predicting the long-term dynamic competition of sulfidogens and methanogens for electrons. This could explain a long-term of over 200 days needed for the SRB out-competing the MPA, and support speculation that the SRB could finally out-compete both the AcB and the MPA.

Influence of Lower Substrate pH and Retention Time on the Efficiency of a UASB Bioreactor Treating Winery Waste Water

A mesophilic laboratory-scale upflow anaerobic sludge bed (UASB) bioreactor design was evaluated for the treatment of winery waste water. In the first experimental study, the hydraulic retention time (HRT) was shortened from 24 h to 13 h which led to an increase in the organic loading rate (OLR) from 6,34 to 10,12 kgCOD.m-3.d-l. The recovery rate of the bioreactor, in terms of pH stabilisation was much slower for HRT's less then 14 h, suggesting that the optimum operational HRT had been reached. At this HRT the average COD removals were between 93 and 97% and the removal rate was 10,94 kgCOD.m-3.d-1. The second experimental study was the reduction in substrate pH from 7 ,5 to 5,0. This study was performed to investigate the lowest substrate pH that the active microbial population in the bioreactor could handle so as to reduce neutralisation costs and acclimatise the microbes to lower pH's. The lowest operational pH reached was 5,1 with a COD removal of over 90%. This has a considerable impact on the economic aspects of the winery waste water treatment process as neutralisation of the waste water will not be necessary before introducing it to the UASB bioreactor.

USE OF A GRIFFITH TUBE TO EVALUATE THE ANAEROBIC SLUDGE SEDIMENTATION IN A UASB REACTOR TREATING AN EFFLUENT WITH LONG-CHAIN FATTY ACIDS

Abstract - This paper proposes to study the sedimentation characteristics of anaerobic sludge, by determining the settling velocity of sludge granules with the Griffith Tube. This is a simple, low-cost method, suitable for use in full-scale treatment plants. The settling characteristics of sludge from two laboratory-scale UASB reactors fed with saccharose and different concentrations of sodium oleate and sodium stereate were evaluated. Addition of fatty acids caused a gradual destabilization of the system, affecting overall performance. The sedimentation profile changed after addition of fatty acids to the synthetic substrate, decreased sedimentation velocity and increased granule diameter. This behaviour was attributed to the adsorption of fatty acids onto the granules, modifying the diameter, shape and density of these bioparticles. Keywords : Anaerobic process; UASB reactor; Long chain fatty acids; Griffith tube; Sludge settleability. INTRODUCTION Anaerobic wastewater treatment biotechnology has been greatly advanced by the development of the upflow sludge bed reactor concept, such as the up-flow anaerobic sludge bed (UASB) (Kim

Phosphorus removal from UASB reactor effluent by reactive media filtration

ABSTRACT The phosphorus (P) and BOD7 removal performance of an upflow packed bed reactor (PBR) filled with two reactive filter media was studied over 50 weeks. The lower one-fifth of the reactor was filled with calcium–silicate–hydrate (Sorbulite®) and the upper four-fifth with calcium–silicate (Polonite®). A laboratory-scale upflow anaerobic sludge bed reactor (UASB) delivered wastewater to the PBR. A model was developed to describe the gradient in P concentration change in the reactor, based on reaction kinetics. The reaction terms were assumed to follow the Langmuir isotherm, based on the results obtained in a batch test. First, a comparison was made between experimental and simulated results. The capability of the model to forecast P removal capacity was then tested for three hypothetical cases: (i) reactor filled with Sorbulite and Polonite, (ii) reactor filled with only Sorbulite, and (iii) reactor filled with only Polonite. Finally, a sensitivity analysis was performed for the main parameters in the model. The average removal of P and BOD7 from the UASB effluent was 98% and 90%, respectively. The starting pH of the dual-medium effluent was 12.2 and decreased gradually over time to 11.1. The simulation both overestimated and underestimated mean measured P removal but was within the range of maximum and minimum measured values. The hypothetical cases revealed that most P was removed by Polonite due to calcium phosphate precipitation. The removal capacity of the two filter materials and their layer height in the reactor were the most sensitive parameters in the simulation.

Symbiotic relationship analysis of predominant bacteria in a lab-scale anammox UASB bioreactor

In order to provide the comprehensive insight into the key microbial groups in anaerobic ammonium oxidation (anammox) process, high-throughput sequencing analysis has been used for the investigation of the bacterial communities of a lab-scale upflow anaerobic sludge bed (UASB) anammox bioreactor. Results revealed that 109 operational taxonomic units (OTUs; out of 14,820 reads) were identified and a domination of anammox bacteria of Candidatus Kuenenia stuttgartiensis (OTU474, 35.42%), along with heterotrophs of Limnobacter sp. MED105 (OTU951, 14.98%), Anerolinea thermophila UNI-1 (OTU465 and OTU833, 6.60 and 3.93%), Azoarcus sp. B72 (OTU26, 9.47%), and Ignavibacterium sp. JCM 16511 (OTU459, 8.33%) were detected. Metabolic pathway analysis showed that Candidatus K. stuttgartiensis encountered gene defect in synthesizing a series of metabolic cofactors for growth, implying that K. stuttgartiensis is auxotrophic. Coincidentally, the other dominant species severally showed complete metabolic pathways with full set gene encoding to corresponding cofactors presented in the surrounding environment. Furthermore, it was likely that the survival of heterotrophs in the autotrophic system indicates the existence of a symbiotic and mutual relationship in anammox system.

Anaerobic digestion of alkaline bleaching wastewater from a kraft pulp and paper mill using UASB technique

Anaerobic digestion of alkaline kraft elemental chlorine-free bleaching wastewater in two mesophilic, lab-scale upflow anaerobic sludge bed reactors resulted in significantly higher biogas production (250 ± 50 vs. 120 ± 30 NmL g ) and reduction of filtered total organic carbon (fTOC) (60 ± 5 vs. 43 ± 6%) for wastewater from processing of hardwood (HW) compared with softwood (SW). In all cases, the gas production was likely underestimated due to poor gas separation in the reactors. Despite changes in wastewater characteristics, a stable anaerobic process was maintained with hydraulic retention times (HRTs) between 7 and 14 h. Lowering the HRT (from 13.5 to 8.5 h) did not significantly affect the process, and the stable performance at 8.5 h leaves room for further decreases in HRT. The results show that this type of wastewater is suitable for a full-scale implementation, but the difference in methane potential between SW and HW is important to consider both regarding process dimensioning and biogas yield optimization.

Treatment of high-strength synthetic sewage in a laboratory-scale upflow anaerobic sludge bed (UASB) with aerobic activated sludge (AS) post-treatment

Performance of a combined system up-flow anaerobic sludge blanket (UASB) followed by aerobic treatment activated sludge (AS) for removal of carbonaceous and nitrogenous contaminants at an average temperature of 25°C was investigated. The combined system was fed with high strength synthetic sewage having chemical oxygen demand (COD) of 2500 mg L−1. The organic loading rate (OLR) of the UASB reactor was increased gradually from 1.1 to 3.8 gCOD Lr −1 d−1. At steady state condition, the UASB reactor achieved removal efficiency up to 83.5% of total COD (CODtot), 74.0% of volatile fatty acid (VFA) and 94.0% of protein. The combined system performed an excellent organic removal pushing the overall removal efficiency of CODtot, VFA and protein to 91.0%, 99.9% and 98.2%, respectively. When the OLR of the UASB increased to 4.4 g COD Lr −1 d−1, the UASB was overloaded and; thus, its effluent quality deteriorated. In respect to nitrogen removal, both partial nitrification and complete nitrification took place in aerobic post-treatment. When the dissolved oxygen (DO) concentration was >2.0 mg L−1, complete nitrification (period B) occurred with an average nitrification efficiency of 96.2%. The partial nitrification occurred due to high OLR to AS during the overloading event (period A) and when DO concentration was <2.0 mg L−1 (period C). The maximum accumulated nitrite concentration in periods A, B and C were 90.0, 0.9 and 75.8 mg NO− 2 -N L−1, respectively. The nitrogen balance results of periods A and C indicated that there was a discrepancy between the amount of ammonium nitrogen removed and the amount of oxidized nitrogen formed. This suggests the occurrence of simultaneous nitrification/denitrification (SND) in aerobic post-treatment.

Influence of effluent recirculation on the performance of Anammox process

A study employing two parallel, laboratory-scale upflow anaerobic sludge bed (UASB) reactors (R1 without and R2 with effluent recirculation) was performed to explore the impact of effluent recirculation with various recirculation ratios (r) on the performance of the anaerobic ammonium oxidation (Anammox) process. When the influent total nitrogen (TN) level was at 700mgL−1, the nitrogen removal rate of R2 (r=0.5,4,5) was stable at approximately 3.5kgm−3day−1, which is higher than the value of 2.9kgm−3day−1 for R1. This performance showed that the recirculation of effluent promoted the efficiency and stability of the Anammox process. The specific Anammox activity of the biomass in R2 reached 13.3mg-TNg−1-VSSh−1, which is 34.3% higher than that in R1. The dilution effect and the hydraulic shear force enhanced the performance of the Anammox reactor. However, effluent recirculation was prone to causing variation in the influent qualities. The variation in the substrate ratio did not significantly affect the stoichiometry of the Anammox reaction or the substrate removal capacity, but the high pH resulting from effluent recirculation, with the corresponding high free ammonia concentration, appeared to hinder the further improvement of Anammox performance.

Effect of chitosan on UASB treating POME during a transition from mesophilic to thermophilic conditions

The effects of chitosan addition on treatment of palm oil mill effluent were investigated using two lab-scale upflow anaerobic sludge bed (UASB) reactors: (1) with chitosan addition at the dosage of 2mg chitosan per g volatile suspended solids on the first day of the operation (R1), (2) without chitosan addition (the control, R2). The reactors were inoculated with mesophilic anaerobic sludge which was acclimatized to a thermophilic condition with a stepwise temperature increase of 5°C from 37 to 57°C. The OLR ranged from 2.23 to 9.47kg COD m−3day−1. The difference in biogas production rate increased from non-significant to 18% different. The effluent volatile suspended solids of R1 was 65mgl−1 lower than that of R2 on Day 123. 16S rRNA targeted denaturing gradient gel electrophoresis (DGGE) fingerprints of microbial community indicated that some methanogens in the genus Methanosaeta can be detected in R1 but not in R2.

Applicability of Anaerobic Digestion Model No. 1 (ADM1) for a specific industrial wastewater: Opium alkaloid effluents

Opium alkaloid processing industries are quite limited throughout the world since cultivation and processing of opium are not practiced in most of the developed countries. However, wastewater generated from this industry is known to be highly polluted with considerable environmental impacts. High rate anaerobic digestion processes are effective in the treatment of opium alkaloid wastewaters that are characterized with relatively low pH and high soluble COD content. Experimental results obtained from the anaerobic treatability studies conducted with lab-scale upflow anaerobic sludge bed reactor (UASB) were simulated by the IWA Anaerobic Digestion Model No. 1 (ADM1). A model based influent characterization which provided consistent results was performed. The validation results indicated that the calibrated ADM1 was able to predict the experimental results of effluent COD, pH, methane and biogas flows with reasonable accuracy. This study demonstrates a useful approach for the implementation of the ADM1 for the treatment of a specific industrial wastewater.

Maximum removal rate of propionic acid as a sole carbon source in UASB reactors and the importance of the macro- and micro-nutrients stimulation

The maximum propionic acid (HPr) removal rate (R HPr) was investigated in two lab-scale Upflow Anaerobic Sludge Bed (UASB) reactors. Two feeding strategies were applied by modifying the hydraulic retention time (HRT) in the UASB HRT and the influent HPr concentration in the UASB HPr, respectively. The experiment was divided into three main phases: phase 1, influent with only HPr; phase 2, HPr with macro-nutrients supplementation and phase 3, HPr with macro- and micro-nutrients supplementation. During phase 1, the maximum R HPr achieved was less than 3 g HPr-COD L −1 d −1 in both reactors. However, the subsequent supplementation of macro- and micro-nutrients during phases 2 and 3 allowed to increase the R HPr up to 18.1 and 32.8 g HPr-COD L −1 d −1, respectively, corresponding with an HRT of 0.5 h in the UASB HRT and an influent HPr concentration of 10.5 g HPr-COD L −1 in the UASB HPr. Therefore, the high operational capacity of these reactor systems, specifically converting HPr with high throughput and high influent HPr level, was demonstrated. Moreover, the presence of macro- and micro-nutrients is clearly essential for stable and high HPr removal in anaerobic digestion.

Anaerobic methanethiol degradation and methanogenic community analysis in an alkaline (pH 10) biological process for liquefied petroleum gas desulfurization

Anaerobic methanethiol (MT) degradation by mesophilic (30 degrees C) alkaliphilic (pH 10) communities was studied in a lab-scale Upflow Anaerobic Sludge Bed (UASB) reactor inoculated with a mixture of sediments from the Wadden Sea (The Netherlands), Soap Lake (Central Washington), and Russian soda lakes. MT degradation started after 32 days of incubation. During the first 252 days, complete degradation was achieved till a volumetric loading rate of 7.5 mmol MT/L/day, and sulfide, methane, and carbon dioxide were the main reaction products. Temporary inhibition of MT degradation occurred after MT peak loads and in the presence of dimethyl disulfide (DMDS), which is the autooxidation product of MT. From day 252 onwards, methanol was dosed to the reactor as co-substrate at a loading rate of 3-6 mmol/L/day to stimulate growth of methylotrophic methanogens. Methanol was completely degraded and also a complete MT degradation was achieved till a volumetric loading rate of 13 mmol MT/L/day (0.77 mmol MT/gVSS/day). However, from day 354 till the end of the experimental run (day 365), acetate was formed and MT was not completely degraded anymore, indicating that methanol-degrading homoacetogenic bacteria had partially outcompeted the methanogenic MT-degrading archea. The archeal community in the reactor sludge was analyzed by DGGE and sequencing of 16S rRNA genes. The methanogenic archea responsible for the degradation of MT in the reactor were related to Methanolobus oregonensis. A pure culture, named strain SODA, was obtained by serial dilutions in medium containing both trimethyl amine and dimethyl sulfide (DMS). Strain SODA degraded MT, DMS, trimethyl amine, and methanol. Flow sheet simulations revealed that for sufficient MT removal from liquefied petroleum gas, the extraction and biological degradation process should be operated above pH 9.

Anaerobic methanethiol degradation in upflow anaerobic sludge bed reactors at high salinity (≥0.5 M Na+)

The feasibility of anaerobic methanethiol (MT) degradation at elevated sodium concentrations was investigated in a mesophilic (30 degrees C) lab-scale upflow anaerobic sludge bed (UASB) reactor, inoculated with estuarine sediment originating from the Wadden Sea (The Netherlands). MT was almost completely degraded (>95%) to sulfide, methane and carbon dioxide at volumetric loading rates up to 37 mmol MT x L(-1) x day(-1), 0.5 M sodium (NaCl or NaHCO(3)) and between pH 7.3 and 8.4. Batch experiments revealed that inhibition of MT degradation started at sodium (both NaCl and NaHCO(3)) concentrations exceeding 0.8 M. Sulfide inhibited MT degradation already around 3 mM (pH 8.3).

Coagulation and Precipitation as Post-Treatment of Anaerobically Treated Primary Municipal Wastewater

The main objective of this study was to investigate the feasibility of coagulation as a post-treatment method of anaerobically treated primary municipal wastewater. Both mesophilic and ambient (20 degrees C) temperature conditions were investigated in a laboratory-scale upflow anaerobic sludge bed (UASB) reactor. In addition, optimization of the coagulant, both in terms of type and dose, was performed. Finally, phosphorus removal by means of aluminum and iron coagulation and phosphorus and ammonia nitrogen removal by means of struvite precipitation were studied. Anaerobic treatment of primary effluent at low hydraulic retention times (less than 15 hours) resulted in mean chemical oxygen demand (COD) removals ranging from 50 to 70%, while, based on the filtered treated effluent, the mean removals increased to 65 to 80%. Alum coagulation of the UASB effluent gave suspended solids removals ranging from approximately 35 to 65%. Turbidity removal reached up to 80%. Remaining COD values after coagulation and settling were below 100 mg/L, while remaining total organic carbon (TOC) levels were below 50 mg/L. Filterable COD levels were generally below 60 mg/L, while filterable TOC levels were below 40 mg/L. All coagulants tested, including prepolymerized aluminum and iron coagulants, demonstrated similar efficiency compared with alum for the removal of suspended solids, COD, and TOC. Regarding struvite precipitation, optimal conditions for phosphorus and nitrogen removal were pH 10 and molar ratio of magnesium: ammonia-nitrogen: phosphate-phosphorus close to the stoichiometric ratio (1:1:1). During struvite precipitation, removal of suspended solids reached 40%, while turbidity removal reached values up to 80%. The removal of COD was approximately 30 to 35%; yet, when removal of organic matter was based on the treated filterable COD, the removal increased to approximately 65%. In addition, nitrogen was removed by approximately 70%, while phosphorus removal ranged between approximately 30 and 45% on the basis of the initial phosphorus concentration. Finally, size fractionation of the organic matter (COD) showed that the various treatment methods were capable of removing different fractions of the organic matter.

Modelling anaerobic digestion acclimatisation to a biodegradable toxicant: application to cyanide

The observed acclimatisation to biodegradable toxicants in anaerobic cassava wastewater treatment is explained by modelling anaerobic cyanide degradation. A complete degradation pathway is proposed for cyanide. Cyanide degradation is modelled as enzymatic hydrolysis to formate and ammonia. Ammonia is added to the inorganic nitrogen content of the digester while formate is degraded by the hydrogenotrophic methanogens. Cyanide irreversible enzyme inhibition is modelled as an inhibition factor to acetate uptake processes. Cyanide irreversible toxicity is modelled as a decay factor to the acetate degraders. Cyanide as well as added phosphorus buffer solution were considered in the chemical equilibrium calculations of pH. The observed reversible effect after acclimatisation of sludge is modelled by a population shift between two aceticlastic methanogens that have different tolerance to cyanide toxicity. The proposed pathway is added to the IWA Anaerobic Digestion Model no.1 (ADM1). The ADM1 model with the designed extension is validated by an experiment using three lab-scale upflow anaerobic sludge bed reactors which were exposed to different cyanide loadings.

Behavior of an Up-flow Anaerobic Sludge Bed (UASB) reactor at extreme salinity

High salinity is one of the most well known inhibitors and salt concentrations above 1% are reported as highly saline. Salt may be found in the main collectors of municipal sewer systems which carry the risk of seawater infiltration and where domestic wastewaters are treated with landfill leachates such cases in Istanbul. Discharging of leachates into sewerage would also result in additional ammonium loads in Municipal Treatment Plants (MTPs). In this study, synthetic wastewater was fed to a lab-scale Upflow Anaerobic Sludge Bed (UASB) reactor operated at a constant hydraulic retention time (HRT) of 1 day. The salt concentration was increased artificially whereas the ammonium concentration was fixed at 1000 mg/l throughout the study. Results indicated no significant inhibition up to 2% salinity + 1000 mg/l NH4 and TOC removals could reach up to 88%. Besides, effective total biogas productions having methane content of 84% could be achieved. It is obvious that anaerobic microorganisms (especially methanogens) could well adapt to high salinity ratios. No inhibition at high ammonium concentration might have been attributed to the fact that the pH in the system was not high enough for the formation of free ammonia. Investigation of the combined effect of high salinity and ammonium was carried on by increasing the salinity to 3% in the study. Results indicated that TOC removals decreased to around 56% and 3% salinity caused . a sharp decrease both in organic material removal and total biogas production. Thus the critical salinity level was determined as 3% for the methanogens in the mesophilic anaerobic digesters.

Effect of high salinity on anaerobic treatment of low strength effluents

In anaerobic treatment, it is obligatory to know the effect of potentially inhibitory compounds due to the fact that methane formation may retard severely and may proceed slower than organic acid production. One of the most important inhibitory substances in anaerobic treatment is high salinity. In many cases, the main collectors of a municipal sewer system should have been built in the coastal zone and below the ground water level due to the available topography of wastewater catchments area, which is carrying the risk of seawater infiltration. Besides, one of the most convenient methods for leachate control is to treat landfill leachates with domestic wastewaters in the central municipal wastewater treatment plants such as in Istanbul. Thus, the nitrogen load of the treatment plants increase significantly. In this study, the effects of high salinity and ammonium nitrogen levels on mesophilic anaerobic tretament processes were investigated. In the first part of the study, high salinity effect on anaerobic treatment was investigated by feeding synthetic wastewater containing high salinity between 0.15%-1.5% ratios. In the second part of the study, the simultaneous effect of high salinity and ammonia (1.5% salinity+1,000 mg NH3/l) was examined by a lab-scale Upflow Anaerobic Sludge Bed Reactor (UASBR). Results indicated no significant inhibition in both cases and effective COD removals (89%) and total biogas productions having methane content of 84% could be achieved.