Research Laboratory Research Articles

No Experience Required: Inclusive Undergraduate Research in Computational Biophysical Chemistry

ABSTRACT Undergraduate research is a key tool for recruitment and, more importantly, retention of science, technology, engineering, and math students. Unfortunately, many students are not aware of or do not take advantage of these opportunities and, at best, often wait until late in their college careers. For my undergraduate computational biophysical chemistry research lab I have established a no-experience-required policy that allows me to recruit and mentor more research students. It also allows me to be more inclusive and expand the pool of students who have access to research. This also results in a more diverse research group, which is particularly important at a primarily undergraduate institution that has a diverse population with a high percentage of first-generation students. Here, I lay out the procedures I use to recruit and train students of all levels for my research lab, as well as the research products produced by students and myself.

Abstract 4136984: Low Occurence of Fetal Extranodal Findings and AV Interval Prolongation in Prospectively Followed High Titer Anti-Ro Pregnancies

. Background: Third-degree atrioventricular block (AVB) is the most common manifestation of Anti-Ro antibody associated fetal cardiac disease. Extranodal findings of isolated cardiac echogenicity, valvulitis with insufficiency and AV interval prolongation have been reported but not described in large prospective series. Aims: To report the occurrence and outcomes of extranodal findings in subjects followed prospectively since 2020 in STOP BLOQ (Surveillance and Treatment to Prevent Fetal AV block Likely to Occur Quickly) and the Registry for Neonatal Lupus (RNL). Methods: We reviewed fetal echo reports from pregnant STOP BLOQ and RNL Anti-Ro antibody subjects. Pregnancies with high (>1000 EU for anti-Ro52 or 60) antibody titers measured in a core research lab underwent surveillance with home fetal heart rate monitoring (FHRM) 3x/day and weekly or bi-weekly fetal echos from 17- 26 gestational weeks. Low titer subjects underwent echo or no surveillance based on local site protocol. We evaluated cardiac function, effusions, cardiac echogenicity and its location, any tricuspid (TV) or mitral (MV) insufficiency trivial or >trivial) and AV conduction times (<150, 150-170 or >170 ms) Results: Echo reports were available in 622 prospectively followed pregnancies (376 high (40/376 with a previously affected child) and 246 low-titer pregnancies. All had subjectively normal ventricular function and none had effusions. Seven fetuses, 2 low and 5 high titer at 19 (range 16.7-21.7) weeks demonstrated cardiac echogenicity (n=6), AV or semilunar valve insufficiency (n=2) or AV interval 150-170 ms (n=2) (Table). Subjects 1 and 2, both high titer and treated with prophylactic Plaquenil (400 mg/d before 10 gestational weeks), had prior fetal AVB. Subject #1 had normal AV conduction but MV and TV echogenicity and moderate insufficiency of both valves which resolved in days after IVIG and dexamethasone (dex) treatment, but 3° AVB developed at 19 weeks after dex wean. Subjects 2-7 received no treatment and extranodal findings did not progress to cardiac dysfunction or AVB. During the same time period, 10 high titer subjects developed 2° or 3° AVB. Conclusion: Anti-Ro associated extranodal findings are rare, occurring in 1% of pregnancies overall and in 5% of pregnancies with a previously affected child. Unlike AVB, extranodal findings can occur in low titer pregnancies. The role of treatment for isolated extranodal findings in the absence of cardiac dysfunction is unclear.

Abstract 4137678: Maximal Oxygen Uptake Prediction From Resting and Submaximal Variables of Cardiopulmonary Exercise Testing by Using Machine Learning Methods

Background: Cardiorespiratory fitness, as measured by maximal oxygen consumption (VO2max) during a cardiopulmonary exercise test (CPET), is a prognostic indicator for longevity and adverse cardiovascular event prevention. This study aims to formulate a regression model utilizing resting and submaximal variables during CPET evaluation in the general population to predict VO2max. Methods: We used 13,535 CPET results on cycle ergometer collected by the UCLA Exercise Physiology Research Laboratory over eight years. Patients were divided into a normal group (NG; n=1,400) and an other group (OG; n=12,135). The inclusion criteria for the NG were: the absence of any known clinical diagnosis, BMI <30, no current use of beta blocker, and VO2max >85% of the predicted value for age and sex (Wasserman equation). OG refers to all other patients not in NG. Models were trained and evaluated for each group using stratified 5-fold cross-validation. We also trained different models using only resting variables (R-VARS) and resting and submaximal exercise variables (R+S-VARS). Feature importance was assessed using Shapely additive explanations (SHAP) values to identify how the feature related to the VO2max prediction. Results: The regression models were trained on the NG, OG, and total (N+OG) groups. The optimal models were Bayesian Ridge for the NG and Light Gradient Boosting Machine for the other two groups. The mean (standard deviation) R2 when using only R-VARS was 0.67(0.037) for the NG, 0.54 (0.014) for the OG, and 0.55 (0.009) for the N+OG. When using R+S-VARS, performance increased to 0.82 (0.014) for the NG, 0.80 (0.010) for the OG, and 0.8 (0.008) for the N+OG. Chronotropic index (ci), body mass index (BMI), VO2 at the first ventilatory threshold (VO2h1kg), minute ventilation at the second ventilatory threshold (VEH2), and forced expiratory volume (FEV1) were important features across the models trained with R+S-VARS. CI, BMI, and VEH2 had a negative effect, while VO2h1kg, FEV1 had positive effect on VO2max prediction. Conclusion: Our VO2max prediction model demonstrated remarkable accuracy in this dataset representing a generally healthy, non-athlete population using R+S-VARS. Particularly noteworthy was the enhanced performance within subgroups exhibiting a lower VO2max. This methodology offers a means to assess VO2max for individuals who might not achieve maximal exhaustion during CPET due to non-cardiopulmonary reasons.

Constraining light dependency in modeled emissions through comparison to observed biogenic volatile organic compound (BVOC) concentrations in a southeastern US forest

Abstract. Climate change will bring about changes in meteorological and ecological factors that are currently used in global-scale models to calculate biogenic emissions. By comparing long-term datasets of biogenic compounds to modeled emissions, this work seeks to improve understanding of these models and their driving factors. We compare speciated biogenic volatile organic compound (BVOC) measurements at the Virginia Forest Research Laboratory located in Fluvanna County, VA, USA, for the year 2020 with emissions estimated by the Model of Emissions of Gases and Aerosols from Nature version 3.2 (MEGANv3.2). The emissions were subjected to oxidation in a 0-D box model (F0AM v4.3) to generate time series of modeled concentrations. We find that default light-dependent fractions (LDFs) in the emissions model do not accurately represent observed temporal variability in regional observations. Some monoterpenes with a default light dependence are better represented using light-independent emissions throughout the year (LDFα-pinene=0, as opposed to 0.6), while others are best represented using a seasonally or temporally dependent light dependence. For example, limonene has the highest correlation between modeled and measured concentrations using an LDF =0 for January through April and roughly 0.74–0.97 in the summer months, in contrast to the default value of 0.4. The monoterpenes β-thujene, sabinene, and γ-terpinene similarly have an LDF that varies throughout the year, with light-dependent behavior in summer, while camphene and α-fenchene follow light-independent behavior throughout the year. Simulations of most compounds are consistently underpredicted in the winter months compared to observed concentrations. In contrast, day-to-day variability in the concentrations during summer months are relatively well captured using the coupled emissions–chemistry model constrained by regional concentrations of NOX and O3.

Violation of the handling rules of pathogens: discourse on the definition of the subject of the criminal offense

The relevance of the study is determined by the growing importance of ensuring the biological security of the state, in particular, by criminal legal means in the context of strengthening control over the activities of research, diagnostic and production microbiological laboratories that work with pathogenic material, as well as by the uncertainty of the criminal legal doctrine regarding the mandatory signs of the composition of the criminal offense under Art. 326 of the Criminal Code of Ukraine. The purpose of the article is to develop a scientifically grounded approach to the understanding of the subject of violation of the handling rules of microbiological objects that are pathogens of dangerous diseases, and the responsibility for which is provided for by Article 326 of the current Criminal Code of Ukraine, as well as by Articles 5.1.12, 5.1.13 and 5.1.19 of the draft of the new Criminal Code of Ukraine. To achieve this goal, the following scientific methods were used: hermeneutical, normative-dogmatic, formal-logical, system-structural. The provisions of international and national legislation of Ukraine, which determine the content of the subject of the relevant criminal offense, were analyzed. It was found out that, despite the dual interpretation, the term “biological agents is used both in the current and in the prospective legislative acts of Ukraine, and the lists of dangerous biological agents are approved by the Government and the Ministry of Health of Ukraine. In the absence of the legal definition of the term “infectious agents” and the absence of any normative defined lists of such agents, the use of this term in the construction of the relevant criminal law norms is considered to be questionable. The meaning of the concept of “biotechnology products,” which was used by the authors of the draft of the new Criminal Code of Ukraine to criminalize the violation of the handling rules of pathogens, has been determined. It was found out that there is no legally defined list of biotechnology products, which significantly expands the meaning of this concept. It was argued that the application of the definition of “biotechnology products” in a prospective codified criminal legal act will create unjustified competition of norms and will negatively affect the qualification of relevant criminal offenses. The optimal version of the disposition of the criminal legal norm on the violation of the mentioned rules of biological safety in terms of determining the subject of this criminal offense has been given.

The Effect of Giving Soursop Fruit Juice (Annona muricata, L.) on Hemoglobin Levels in Wistar Bunting Strain Rats Exposed to Cigarette Smoke

Background: Anemia in pregnancy can be caused by exposure to cigarette. A large number of free radicals can cause the number of erythrocytes to decrease. It takes a component to neutralize free radicals, namely antioxidants obtained from various fruits. Soursop contains sources of antioxidants such as flavonoid compounds, polyphenols, and vitamin C. Researchers want to know whether soursop juice can be used as an alternative antioxidant to prevent the decrease in hemoglobin levels of pregnant women. Objective: To determine the effect of giving soursop juice on hemoglobin levels of pregnant white rats with exposure to cigarette smoke. Methods: The experimental laboratory research was designed using the Randomized Post Test Only Control Group Design method. The experimental animal samples were 20 pregnant female Wistar (Rattus norvegicus) rats composed of two control and three treatment groups. Rats were given soursop fruit juice from the first day of rearing until the 18th day of gestation and exposed to cigarette smoke from the 6th day until the 18th. Then blood samples were taken from the mother's heart to measure the hemoglobin level. Results: The results of the hemoglobin level test showed that there was difference between the treatment group and control group. There is an increasing trend from the postitive control group to treatment group. Conclusion: Giving soursop fruit juice can prevent the decrease in hemoglobin levels of pregnant rats exposed to cigarette smoke. Keywords: anemia, free radicals, antioxidants, vitamin C.

Lunar Farside Samples Returned by Chang’E-6 Mission: Significance for Understanding the South Pole-Aitken Basin Stratigraphic History

The Chang’E-6 (CE-6) mission, launched by China on 2024 May 3, marks the first successful retrieval of samples from the farside of the Moon, specifically from the southern mare plain of the Apollo basin within the South Pole-Aitken (SPA) Basin. This study aims to provide a comprehensive geological analysis of the Apollo basin, focusing on the Apollo basin materials that CE-6 may have collected. By examining the regional geology, surface mineralogy, thorium (Th) abundance, and crustal thickness, we seek to understand the implications of these materials for the SPA basin’s structure and stratigraphic history. We find that the Apollo basin exhibits significant lateral compositional variations, with Mg-rich noritic materials predominantly in the southwestern portion and more feldspathic materials in the northeast, probably due to the fact that the Apollo basin is situated on different structures of the underlying SPA basin. Those Mg-rich materials, likely representing deep lithologies from local lower crust, are associated with low Th abundance, suggesting a KREEP-poor origin. The inclusion of foreign materials in the CE-6 samples increases the complexity while also enhancing their research potential. In addition to the local mare basalts, Mg-rich noritic materials associated with the Apollo basin and Th-rich materials originating from the SPA basin may also have been collected by CE-6. Laboratory research of these diverse lithologies will contribute to unraveling the characteristics of the farside mantle, the geological history of the giant SPA basin, as well as the cause of the substantial asymmetry between the lunar nearside and farside.

RBIO-03. A SMALL ANIMAL RADIATION RESEARCH PLATFORM (FLASH-SARRP) TO MEASURE IMMUNOLOGICAL EFFECT OF ULTRA HIGH DOSE RATE (FLASH) RADIOTHERAPY

Abstract Patients who undergo radiation therapy (RT), particularly fractionated courses, often develop lymphopenia. Clinical outcome for various cancer types, including brain tumors, identifies lymphopenia as a negative prognostic factor. The mechanism for RT induced lymphopenia is unclear. Simulated models suggest the volume of blood flowing through a radiation field could play a crucial role in death of radiosensitive lymphocytes. FLASH RT is ultrahigh dose rate administration of RT, >100 times faster dose rate than standard RT that has been observed to improve the therapeutic ratio in preclinical models by preferential normal tissue sparing. Hypothesized mechanisms include chemical reactions depleting oxygen in well oxygenated normal cells creating radioresistance, differing pro-inflammatory signaling impacting tumor and normal tissue, and less impact on circulating immune cells consequent to decreased proportion exposed during a treatment session which may enhance antineoplastic response. We designed the novel FLASH kV x-ray cabinet system for preclinical laboratory research (FLASH-SARRP) to deliver ultra-high dose rates up to 100 Gy/s as well as conventional dose rates of 1.0 Gy/s to facilitate comparative studies. A custom designed docking system was developed to reproducibly position and immobilize mouse for stereotactic radiation of the brain. Dose and dose rate measurements were performed with calibrated Gafchromic EBT3 film in solid water with 0.025 mm additional copper filter. FLASH-SARRP system delivered kV x-rays at an average dose-rate of 79.2±2.1Gy/s corresponding to 10 mm depth in the immobilized mouse brain. For the delivery of 15 Gy, treatment duration was 0.2 seconds for FLASH RT administration whereas it was 244 seconds for conventional dose rate. We successfully designed a high-precision platform to study partial brain x-ray FLASH effects in mice and studies are ongoing to assess differential impact on normal brain tissue, circulating cytokines, and immune cell populations for single dose and fractionated administration of FLASH and standard radiotherapy.

Effectiveness of Mango Gedong Gincu Peel Extract Gel (<i>Mangifera indica</i> L.) on Cut Wounds in Male Wistar White Rats (<i>Rattus norvegicus</i>)

Background: A wound is an injury to a part of the body where the skin and the underlying tissue lose continuity. The wound healing is a complex process consisting inflammatory phase, proliferation phase, and maturation phase. Open wounds often occur in everyday life and if left behind and untreated can potentially lead to bacterial infection. Mango gedong gincu peel extract (Mangifera indica L.) contains flavonoids, tannins, phenols, triterpenoids, steroids, alkaloids, and saponins which have roles in wound healing. Aims: To analyses the effectiveness of mango gedong gincu peel extract gel (Mangifera indica L.) against the healing of cut wounds in male wistar white rats (Rattus norvegicus). Methods: The study was an experimental study with a post-test only control group design using 25 male wistar white rat as the subject and was carried out in the FK UGJ Research Laboratory. The study used five groups : negative control group, positive control group, and treatment group using mango gedong gincu peel extract gel (Mangifera indica L.) with concentrations of 5%, 10%, and 15%. Results: The results of the study revealed that mango (Mangifera indica L.) gedong gincu peel is a source of various phytochemical such as flavonoids, tannins, phenols, triterpenoids, steroids, alkaloids, and saponins. This study showed that the 5% and 10% concentration treatment groups had the same average length measurement wound of 0.86 cm. The fastest observation of erythema was the 5% concentration group with a healing rate of 8 days, in the observation of edema the 10% concentration group was the fastest of 6 days, and the observation long of healing time was 5% and 10% concentration had the same rate of 11 days. Conclusion: Mango gedong gincu peel extract gel (Mangifera indica L.) was effective against wound healing with optimum concentrations of 5% and 10% which gives the best healing effect against wounds in male wistar white rats (Rattus norvegicus). Received: 25 September 2024 | Reviewed: 22 October 2024 | Revised: 05 November 2024 | Accepted: 09 November 2024.

MycoTWIN Working Group Discussion: A Multi-Actor Perspective on Future Research Directions for Mycotoxins and Toxigenic Fungi Along the Food and Feed Chain

Mycotoxin research is facing unprecedented challenges, starting from the urgent need to cope with the consequences of climate change, the global shortage of grain due to unstable political scenarios, and the major transformation of the supply chains after the COVID-19 pandemic. In this scenario, the mycotoxin contamination of human and animal foods is still unavoidable, thus representing a major challenge to global food security. Next to this, the shift to sustainable and circular food production might be accompanied by an increase in food safety issues involving mycotoxins, e.g., when new technologies are applied to reuse side streams from the food industry, it is not known if and how mycotoxins accumulate in these by-products. MycoTWIN is an EU-funded Horizon 2020 project which fosters knowledge transfer and scientific cooperation within the Mediterranean area, involving worldwide experts, decision makers, and stakeholders in the field of mycotoxigenic fungi and mycotoxins. The MycoTWIN project hosted working group meetings, whose aim was to propose operational plans and/or scientific strategic plans to shape the future research directions to better cope with these challenges. In the working group cycle “Future proof approaches for the management of toxigenic fungi and associated mycotoxins along the food chain”, a multi-actor group was guided in co-creation exercises to elaborate on future research directions and propose relevant actions to be implemented for the present to long-term time periods. The discussion focused on three main topics relevant to the assessment and management of risks associated with mycotoxins and toxigenic fungi: (i) needs for the harmonization of molecular and chemical methods and data analysis, (ii) from lab research to marketable solutions: how to fill the gap, and (iii) gaps in data quality for risk assessment.

Influence of particle density on turbulence characteristics over a rough surface

The objective of the study is to use a 3D Acoustic Doppler Velocimeter (ADV) to gauge the typical flow and turbulence characteristics within a non-uniform open channel. The findings of experimental examinations of the subcritical flow along the channel are presented in this work. The behavior of sand grains in turbulent open channel flow across porous and rough bed surfaces was examined in laboratory research, and the results were obtained. The properties of turbulent flow, i.e., turbulence intensity, turbulent kinetic energy, and Reynolds shear stresses, are determined from ADV data. The continuity equation and the Reynolds equation of open-channel flow have been used to build theoretical formulations for the velocity distribution and Reynolds stress distribution in the vertical direction. Measured profiles of vertical velocity and Reynolds stress are compared to the derived expressions. The impact of the size of particles on the distribution of mean flow characteristics is discussed. This work provides a novel origin for the profile and analyzes the behavior of the vertical velocity distribution in the region where fully formed turbulence is dominating in open channels using the Navier–Stokes equations. In comparison to other sand roughness, Chopan sand bed (with greater density) exhibits the strongest turbulence intensities in both vertical and streamwise direction just next to the bed when away from the channel boundary. In contrast to flow across a rough surface, the variance ranges between 150 and 250% concerning the channel bed’s roughness type, impacting the velocity triple products that signifies transfer of turbulent kinetic energy.

Learning on a Limb: An outreach module to engage high school students in orthopaedics.

Orthopaedic researchers need new strategies for engaging underrepresented minority (URM) students. Our field has demonstrated noticeable gaps in racial, ethnic, and gender diversity, which inhibit our ability to innovate and combat the severe socioeconomic burden of musculoskeletal disorders. Towards this goal, we designed, implemented, and evaluated Learning on a Limb (LoaL), an orthopaedic research outreach module to teach URM high school students about orthopaedic research. During the 4-h module, students completed hands-on activities to learn how biomechanical testing, microcomputed tomography, cell culture, and histology are used in orthopaedic research. Over 3 years, we recruited 32 high school students from the Greater Philadelphia Area to participate in LoaL. Most participants identified as racial/ethnic or gender minorities in orthopaedic research. Using pre/post-tests, we found that students experienced significant learning gains of 51 percentage points from completing LoaL. In addition to teaching students about orthopaedic research, post-survey data demonstrated that participating in LoaL strongly influenced students' interest in orthopaedic research and scientific confidence. Several students acted on this interest by completing summer research experiences in the McKay Orthopaedic Research Laboratory at the University of Pennsylvania. LoaL instructors also benefited by having the opportunity to "pay it forward" to the next generation of students and build community within their department. Empowering institutions to host modules like LoaL would synergistically inspire URM high school students and strengthen community within orthopaedic departments to ultimately enhance orthopaedic research innovations.

Reducing potential dual-use risks in synthetic biology laboratory research: a dynamic model of analysis

Synthetic biology (SynBio) is an interdisciplinary field that includes biology, genomics, engineering, and informatics. The introduction of machine learning technology reduces the requirement for human participation in SynBio research, which may lead to increased biosafety and biosecurity concerns within the complex human–technology-management system in SynBio laboratories compared with traditional biology. The rapid development of SynBio and machine learning technology has added to the systemic complexity of management and technology subsystems, the risks of which remain unclear. To fully understand and address these risks, there is an urgent need to develop a quantitative model. In this study, three-round Delphi interviews were conducted with SynBio experts who have published articles in top journals (e.g., Nature, Science, Cell, etc.) and applied the system dynamics method to build a quantitative analysis model. The model explores the potential risk factors and their complex relationships in the SynBio research. We analyzed three subsystems (biotechnology, information technology, and management), identifying seven key risk factors. Among these, the Average Experience of Newcomers and Tolerable Error Frequency play an essential role in laboratory management. Notably, the biosafety atmosphere has the greatest impact on reducing error rate and increasing safety awareness, as well as on the infrastructure safety of SynBio laboratory. In addition, we also consider the impact of the progressiveness of machine learning in the SynBio research and find that using more advanced machine learning technology leads to lower instrument safety. This study provides a framework for quantifying potential risk factors in SynBio research and lays a theoretical foundation for future laboratory safety management and the development of a global code of conduct for interdisciplinary scientists.

On the Internal Bremsstrahlung accompanying β-decay and its potential relevance in the application of radioactive sources.

An in-depth analysis of the decay process for β-emitting radionuclides highlights, for some of them, the existence of high-order effects usually not taken into account in literature as considered negligible in terms of energy and yield, and referred to as Internal Bremsstrahlung (IB). This set of β-radionuclides presents, besides their β spectrum, a continuous γ emission due to the Coulomb field braking action on the emitted electron following the decaying nucleus. 
In this work, we review the theoretical and experimental studies on the IB process focusing on its actual importance for the pure β emitters. It emerges that there is no satisfactory model able to reproduce the experimental IB distribution for most of the investigated beta emitters and the several measurements are sometimes at odds with each other. Moreover, as recently demonstrated, the IB process can give a relevant contribution to the physics of beta emitters thus requiring its inclusion in the physics of the beta decay.
A discussion on the importance of considering the IB process in both applicative fields such as nuclear medicine, industrial applications, and research or calibration laboratories, and in other relevant fields of particle physics or astrophysics, such as the research on dark matter or neutrino mass, is presented.

Mergers and Licensing With Horizontal Differentiation

ABSTRACTWe consider a research laboratory that owns a patented process innovation and two firms producing differentiating goods in a Bertrand setting. The laboratory considers the possibility to license the innovation as an outsider patentee or to merge with one of the firms in the industry, becoming an incumbent patentee. Licensing takes place through observable two‐part tariff contracts. We show that the merger is profitable only for small innovations and increases social welfare for both small and large innovations. Even though we allow the royalty to be higher than the size of the innovation, and opposite to the result in a Cournot setting, we find a region where the merger is both profitable and welfare improving. This occurs only for small innovations and sufficiently differentiated goods. The same result arises for consumer surplus which allows us to derive the optimal merger policy: compared with Cournot competition, a Bertrand setting calls for a more lenient merger policy.

Interval Analysis of Volatile Solid (VS) and Biochemical Oxygen Demand (BOD) in Batch Anaerobic Fermentation of Selected Agrowaste

This study investigated the degradation of volatile solids (VS) and biochemical oxygen demand (BOD) during the anaerobic digestion of selected agro-waste, specifically cassava peels, maize husks, pig slurry, and the composite. The objective was to evaluate the biodegradability of these feedstocks, in line with their potential for biogas production. The Research was conducted at the National Centre for Energy Research and Development (NCERD) laboratory, University of Nigeria Nsukka (UNN). Batch reactors were used to conduct experiments over a period of 45 days. The digesters were operated under controlled conditions, and samples were analyzed at three (3) different points: 24 hours, peak of gas production (day 30), and at the end of the digestion (day 45). The results demonstrated that all the substrates exhibited a reduction in VS and BOD in the course of digestion. However, the most significant reduction in VS and BOD occurred around the peak of gas production. This entails that this period is critical for microbial activity and biogas yield. Corn husks had high initial VS and BOD values of 4.73±0.22 % and 92.80±3.26 mg/L respectively. However, that did not translate to higher biogas production compared with the composite that had initial values of 4.34±0.19 % and 88.00±5.50 mg/L respectively. By the end of the digestion, an average VS reduction of 65% and a BOD reduction of 70% were observed across all substrates, indicating a substantial degradation of organic material. Biogas production was better in the composite (72.580±4.53 %), an indication that higher VS and BOD do not automatically translate to higher gas yield. Thus, factors like multiple substrate interaction affect gas yield and process efficiency.

A multi-paradigm approach to characterize dominance behaviors in CD1 and C57BL6 male mice.

Social status and dominance are critical factors influencing well-being and survival across multiple species. However, dominance behaviors vary widely across species, from elaborate feather displays in birds to aggression in chimps. To effectively study dominance, it is essential to clearly define and reliably measure dominance behaviors. In laboratory settings, C57BL/6 mice are commonly used to study dominance due to their stable and linear social hierarchies. However, other mouse strains are also used for laboratory research. Despite substantial evidence for strain effects on behavioral repertoires, the impact of strain on dominance in mice remains largely unstudied. To address this gap, we compared dominance behaviors between CD1 and C57BL/6 male mice across four assays: observation of agonistic behaviors, urine marking, tube test, and a reward competition. We found that CD1 mice demonstrate increased fighting, increased territorial marking through urination, and increased pushing and resisting in the tube test. We used unsupervised machine learning and pose estimation data from the reward competitions to uncover behavioral differences across strains and across rank differences between competing pairs. Of the four assays, urine marking and agonistic behaviors showed the strongest correlation with dominance in both strains. Most notably, we found that CD1 dominance rankings based on the tube test negatively correlated with rankings from all three other assays, suggesting that the tube test may measure a different behavior in CD1 mice. Our results highlight that behaviors can be strain-specific in mice and studies that measure social rank should consider assays carefully to promote reproducibility.Significance Statement Recent studies have highlighted that social dominance can significantly impact behavior and the brain. As such, accurately measuring dominance behavior in laboratory settings is crucial in neuroscience research. In this study, we investigated the consistency of four dominance assays for male mice across two common mouse strains. We find that not all assays result in the same dominance rankings and dominance behaviors differ across strains. Our study sheds light on the importance of considering strains for assay selection, rigor, and reproducibility.

An Overview of the R&D of Flywheel Energy Storage Technologies in China

The literature written in Chinese mainly and in English with a small amount is reviewed to obtain the overall status of flywheel energy storage technologies in China. The theoretical exploration of flywheel energy storage (FES) started in the 1980s in China. The experimental FES system and its components, such as the flywheel, motor/generator, bearing, and power electronic devices, were researched around thirty years ago. About twenty organizations devote themselves to the R&D of FES technology, which is developing from theoretical and laboratory research to the stage of engineering demonstration and commercial application. After the research and accumulation in the past 30 years, the initial FES products were developed by some companies around 10 years ago. Today, the overall technical level of China’s flywheel energy storage is no longer lagging behind that of Western advanced countries that started FES R&D in the 1970s. The reported maximum tip speed of the new 2D woven fabric composite flywheel arrived at 900 m/s in the spin test. A steel alloy flywheel with an energy storage capacity of 125 kWh and a composite flywheel with an energy storage capacity of 10 kWh have been successfully developed. Permanent magnet (PM) motors with power of 250–1000 kW were designed, manufactured, and tested in many FES assemblies. The lower loss is carried out through innovative stator and rotor configuration, optimizing magnetic flux and winding arrangement for harmonic magnetic field suppression. Permanent magnetic bearings with high load ability up to 50–100 kN were developed both for a 1000 kW/16.7 kWh flywheel used for the drilling practice application in hybrid power of an oil well drilling rig and for 630 kW/125 kWh flywheels used in the 22 MW flywheel array applied to the flywheel and thermal power joint frequency modulation demonstration project. It is expected that the FES demonstration application power stations with a total cumulative capacity of 300 MW will be built in the next five years.

Edible Films from the Laboratory to Industry: A Review of the Different Production Methods

AbstractEdible films or coatings as potential replacements for traditional plastic food packaging are a popular subject for research. This article provides a narrative summary of the progress of research into edible food packaging and preparation methods at three different production scales over the past 5 years. First, at the laboratory research level, commonly used coating methods include dipping, vacuum dipping, and spraying. Films are mostly made by solution casting, while 3D printing film technology and electrospinning/electrostatic spraying are emerging technologies in the field. At the pilot level, panning coating, brush coating, and fluidized bed technologies give edible food packaging a more scalable and realistic approach. On an industrial manufacturing scale, in order to improve film formation efficiency, blowing, injection, calendering, etc. are generally based on the extrusion mode. Laboratory-scale research is critical for developing materials and exploring their properties. The technology used for industrial-scale production needs to consider factors such as cost and efficiency. Each technology for making food packaging needs to be selected according to the production purposes and the currently available packaging equipment. However, commonly used edible packaging source materials are generally derived from biological macromolecules such as proteins and polysaccharides. These materials are heated and extruded in industrial production, and their performance will be inferior to that of traditional plastic packaging. In addition, high cost is also a factor that must be considered.

Fungicidal Potentials of Leaf and Bark Extracts of Bryophyllum pinnatum on Cowpea (Vigna unguiculata L.) Seed Fungi

In Nigeria, Cowpea (Vigna unguiculata [L.] Walp) is an important grain legume which besides being a food crop serves as a major source of income for rural households. The action of pathogens on stored seeds causes huge post-harvest and economic losses to cowpea farmers in Nigeria. This research was aimed at isolating and identifying the spoilage pathogens of cowpea seed and testing the potentiality of ethanol extracts of Bryophyllum pinnatum on the isolates. Spoilt cowpea seed samples were purchased from different markets in Anambra State. They were transported to Alpha Research Laboratory, Awka in sterile polythene bags for microbial isolation. The spoilage organisms were isolated from the seeds through microbial culture. The media used for the isolation were Sabouraud Dextrose Agar and Potato Dextrose Agar. Pathogenic fungi were isolated from the spoilt cowpea seeds. The findings indicated that Aspergillus aculeatus, Fusarium solani, Penicillium citrinum, Rhizopus stolonifer, and Aspergillus flavus were present. The pathogenicity test also showed that the above organisms were responsible for cowpea spoilage. These isolates were tested for their in-vitro antifungal activity against the leaf and bark extracts of Bryophyllum Pinnatum. The antifungal activity ethanol extracts of Bryophyllum pinnatum on the isolated microorganisms were investigated using the disc method. Three different concentrations (25%, 50%, 100%) of extracts were used for the test. Fusarium solani showed the highest percentage of inhibition with antifungal medication while Penicillium citrinum showed the least percentage of inhibition with antifungal medication. The antifungal potential of Bryophyllum pinnatum extract on spoilage pathogens of stored cocoyam corms encourages more research on the active ingredient of the plant for easy use by farmers as alternative to commercial/ synthetic fungicides.