Laboratory Plant Research Articles

Influence of the Mixture of Xanthates collectors on the Flottation of Difficult Copper Ici de Oré to Float-Case of Minerals Fill 18 Deposit Shangulowe

This paper presents the results of a study conducted to highlight the influence of the mixture of xanthate collectors on the flotation of copper oxide ores. The Shangolowe deposit sample assayed 3.22% copper and 0.08% cobalt, and by means of mineralogy and chemical analysis the gangue appears dolomitic. Test optimization ratio and the main sulfudizing collector were performed, testing after which the dosage rates of 4000g NaSH / dT ore and 400g KAX /dT ore were used as a reference for further testing. Xanthate collectors namely: KAX, KEX, SIBX and SNPX were tested alone and their specific behaviors were reported. The study showed that KAX increase the recovery and three other collectors improved concentrates quality. They were then taken two to two mixed in the proportions of 25% -75% 50% -50% 75% -25%. It appears from these tests that the mix-KAX/SIBX taken in ratios of 75 and 25%, stood out all tests because it offered good selectivity and improved metallurgical performance. Comparative testing of cleaning under standard conditions and the best test mixture, also confirmed an improvement in the selectivity of 7 points compared to the usual KAX. A comparison of the collector’s costs, helped highlight a desired gain per ton contained copper with the use of mixed collectors which was about 13% compared to the use of KAX alone. Operating conditions and flotation scheme on the test which offered the best metallurgical performance subsequently proposed to the operator, who was consolidating the results obtained previously with cyclic tests in laboratory and pilot plant.

DETERMINING THE PERFORMANCE OF A HEAT EXCHANGER USED IN THE FOOD INDUSTRY

The study of heat transfer in the food industry is as important as in any other industry. Food products are constantly subjected to heat treatments. For this reason, a sunflower food oil, which undergoes heat treatment in the manufacturing process, was chosen for this study. The study was carried out on a laboratory plant and aimed to determine the heat transfer coefficients for three sets of determinations: partial heat transfer coefficient inside - where the warm sunflower oil circulates, partial heat transfer coefficient in the annular space - where cold water circulates and overall heat transfer coefficient, in a tube-in-tube heat exchanger made of copper. The obtained values of heat transfer coefficients were compared with literature data for the same type of food oil. This type of heat exchanger, tube-in-tube, is specific to the food industry. Countercurrent circulation of flows through the appliance was established from the outset, as this type of circulation is preferred from the point of view of determining the performance of heat exchange appliances. Efficiency values were also established for the three sets of experimental determinations. For the calculations, the values for the temperatures of the fluids, both at the inlet and outlet of the apparatus and the values for the volume flow rates of the fluids were required.

Visualization and Control System for a Wastewater Laboratory Plant with Biological Treatment

This paper presents a novel real-time control and electronic instrumentation system (RTC\andEIS) for a prototype plant that uses activated sludge for wastewater treatment. The main contribution in this research was oriented to work on an experimental design with small dimensions and low cost. This design was carried out using two continuous flow storage tanks in which an identification process was carried out with the MATLAB system identification toolbox to know a mathematical model. The mathematical representation obtained is presented as a continuous and a discrete transfer function. To evaluate the performance of the temperature control and instrumentation system, we implemented a simulation of the closed-loop control system and then implemented it using a DAQ, LabVIEW software, electronic devices and the wastewater treatment plant prototype to compare both results. Finally, the secondary treatment effluent was analyzed in a certified water laboratory to have a detailed analysis compared to the initial influent.

Data-driven crop growth simulation on time-varying generated images using multi-conditional generative adversarial networks

BackgroundImage-based crop growth modeling can substantially contribute to precision agriculture by revealing spatial crop development over time, which allows an early and location-specific estimation of relevant future plant traits, such as leaf area or biomass. A prerequisite for realistic and sharp crop image generation is the integration of multiple growth-influencing conditions in a model, such as an image of an initial growth stage, the associated growth time, and further information about the field treatment. While image-based models provide more flexibility for crop growth modeling than process-based models, there is still a significant research gap in the comprehensive integration of various growth-influencing conditions. Further exploration and investigation are needed to address this gap.MethodsWe present a two-stage framework consisting first of an image generation model and second of a growth estimation model, independently trained. The image generation model is a conditional Wasserstein generative adversarial network (CWGAN). In the generator of this model, conditional batch normalization (CBN) is used to integrate conditions of different types along with the input image. This allows the model to generate time-varying artificial images dependent on multiple influencing factors. These images are used by the second part of the framework for plant phenotyping by deriving plant-specific traits and comparing them with those of non-artificial (real) reference images. In addition, image quality is evaluated using multi-scale structural similarity (MS-SSIM), learned perceptual image patch similarity (LPIPS), and Fréchet inception distance (FID). During inference, the framework allows image generation for any combination of conditions used in training; we call this generation data-driven crop growth simulation.ResultsExperiments are performed on three datasets of different complexity. These datasets include the laboratory plant Arabidopsis thaliana (Arabidopsis) and crops grown under real field conditions, namely cauliflower (GrowliFlower) and crop mixtures consisting of faba bean and spring wheat (MixedCrop). In all cases, the framework allows realistic, sharp image generations with a slight loss of quality from short-term to long-term predictions. For MixedCrop grown under varying treatments (different cultivars, sowing densities), the results show that adding these treatment information increases the generation quality and phenotyping accuracy measured by the estimated biomass. Simulations of varying growth-influencing conditions performed with the trained framework provide valuable insights into how such factors relate to crop appearances, which is particularly useful in complex, less explored crop mixture systems. Further results show that adding process-based simulated biomass as a condition increases the accuracy of the derived phenotypic traits from the predicted images. This demonstrates the potential of our framework to serve as an interface between a data-driven and a process-based crop growth model.ConclusionThe realistic generation and simulation of future plant appearances is adequately feasible by multi-conditional CWGAN. The presented framework complements process-based models and overcomes their limitations, such as the reliance on assumptions and the low exact field-localization specificity, by realistic visualizations of the spatial crop development that directly lead to a high explainability of the model predictions.

Online monitoring of continuous aqueous two-phase flotation (ATPF) for the development of an autonomous control strategy

Aqueous two-phase flotation (ATPF) is a separation technique for isolating biological products such as enzymes from crude complex biosuspensions. The continuous operation mode of ATPF allows high throughput while maintaining a high separation efficiency. In this work, a laboratory plant for continuous ATPF with integrated online measurement technology is presented and ATPF experiments with the model enzyme phospholipase A2 are performed. Three electrical conductivity probes allow real time and spatially resolved characterization of phase mixing in the flotation tank caused by rising bubbles. UV/Vis spectroscopy is used to measure the enzyme concentration online at the top phase outlet. The equilibrium concentrations in the top and bottom phase are linearly dependent on the feed concentration, revealing a constant partition coefficient. The experimental results of this work provide the basis for the development of an autonomous feedback controller for the continuous ATPF process.

Operation of a Pilot-Scale Biogas Plant Made of Textile Materials and Application of Its Results to a Full-Sized Demonstration Plant

In small German farms, there is a technically usable potential of cattle manure and pig manure ranging from 153 to 187 million tons of fresh matter per year. Since 2021 and 2023, new incentives under the Renewable Energy Sources Act (EEG) have been promoting biogas production in small farms. These incentives, applicable to biogas plants up to 150 kWel, include direct compensations for plants up to 100 kWel and market premiums for those up to 150 kWel. A small biogas plant made of textile materials was designed for both pilot and full-scale applications. Compared to conventional concrete biogas reactors, these textile-based reactors offer a simplified construction and operation, eliminating the need for specialized civil engineering. The primary objective of this research is to demonstrate the process engineering feasibility of biogas reactors based on textile materials for small farm biogas plants (30 to 75 kWel). Another goal is to design the construction method in such a way that this type of system can be built by farmers themselves after type testing on site. Operational insights were gathered from the laboratory plant with a 300-L digester volume, using cattle manure and clover grass silage. To adapt the system to the biogas reactor made of textile materials, the reactor was designed without a stirrer. These insights were considered in the design and approval procedure of the full-sized demonstration biogas plant made of textile materials. The full-size demonstration plant digesters underwent an approval procedure from local authorities, featuring treatment volumes of 120 m3 for the main biogas reactor and 550 m3 for the digestate reactor in an earth basin style. This new type of biogas plant could be built in small farms for self-sufficiency in electrical and thermal energy or for treating sewage sludge in small-scale communal wastewater treatment and biogas plants.

Design, Placement, and Laboratory and Field Evaluation of Rejuvenated Cold Recycled Asphalt Mixtures

Cold recycled (CR) pavements, the combination of reclaimed asphalt pavement and a recycling agent (foamed or emulsified asphalt) at ambient temperatures, can provide a sustainable method to rehabilitate, reconstruct, and construct roadways. However, CR pavement is not a one-for-one replacement of typical asphalt base mixtures. To utilize CR pavements as an alternative to a typical asphalt base mixture or surface course, additional materials such as rejuvenating agents may be needed. This research focuses on the formulation, plant production, and placement on the National Center for Asphalt Technology pavement test track off-ramp, and laboratory evaluation of three rejuvenated CR mixtures, one foamed asphalt CR mixture, and an engineered emulsion CR mixture. These mixtures were subjected to strength and moisture susceptibility testing, along with the indirect tensile asphalt cracking test (IDEAL-CT) and high-temperature indirect tensile test (HT-IDT). The influence of laboratory production versus plant production and the impact of rejuvenators on mixture performance and density were investigated. International roughness index and rut depth data for the off-ramp sections indicate adequate performance, except for the anionic emulsion with bio-based rejuvenator section. Laboratory test results indicate that the addition of rejuvenators into two of the three CR mixtures increased density and decreased moisture susceptibility and increased the CTIndex by at least 35% compared to non-rejuvenated CR mixtures. HT-IDT strengths revealed that two of the three rejuvenated mixtures performed slightly below the non-rejuvenated mixtures.

Effect of Auxin and Cytokinin on Orchid Subculture Resulting from Coelogyne Crosses

<p>The black orchid or Coelogyne is one of the native orchids of Kalimantan, widely cultivated in Indonesia. Several species of Coelogyne orchids have been crossed to obtain new superior-quality plants. Modifying the media, such as giving PGR, is necessary for good plant multiplication results. This research was conducted by changing MS growing media with ZPT BA and NAA. This experiment aimed to determine the effect of ZPT BA and NAA on the growth of orchid subcultures from a cross between<em> Coelogyne pandhurata </em>and<em> Coelogyne rumphii</em>. The study used a completely randomized design (RAL) with 2 factors, namely ZPT BA and NAA, each having a concentration of 0 ppm, 0.2 ppm; 0.4 ppm, and 0.6 ppm with 16 treatment combinations and 4 replications. They were implemented at the Laboratory Plant Physiology and Biotechnology, Faculty of Agriculture, UNS Surakarta, from November 2020 to March 2021. The variables observed included the number of shoots, plant length, plant weight, number of leaves, number of roots, root length, when roots appeared, and when shoots appeared. The results showed that BA 0.6 ppm gives the best output on the growth of the number of shoots, and NAA concentration of 0,2 ppm gives the best results on the variable number of roots. The combination of BA 0.6 ppm and NAA 0.2 ppm gave the best results on plant weight growth.</p>

Korelasi antar Karakter Hasil Lima Varietas Tanaman Kacang Panjang (Vigna sinensis) di Lingkungan Organik

This study aims to determine the correlation between the yield characteristics of the yard long bean yield, and the superiority of one of the varieties in an organic environment. This research was conducted at the Integrated Field Laboratory and Seed and Plant Breeding Laboratory, Faculty of Agriculture, University of Lampung in July 2021 – November 2021. This experiment was arranged in a Completely Randomized Design (CRD) with 3 replications and then continued with data analysis in the form of linear analysis, analysis of diversity , and significance test. The observed variables included flowering age, pod color, pod harvesting age, pod length, pod diameter, number of seeds per pod, sugar content, number of productive branches, number of good pods, total number of seeds, and distance between locules. The results showed that the pod length and the number of seeds per pod (r = 0.66). Total pod weight was positively correlated with the number of productive branches (r = 0.83), the number of good pods (r = 0.96), and the total number of pods (r = 0.96). The number of productive branches correlated positively with the number of good pods (r = 0.87) and the total number of pods (r = 0.86). The number of good pods variable has a positive correlation with the total number of pods (r = 0.98). The Cantonese Tavi variety has an advantage in the number of good pods produced in an organic environment. Keywords: Correlation, Organic Environment.

Six-orders-of-magnitude-spanning dispersion measurement via Kalman filtering-aided white-light interferometry.

Dispersion plays a great role in ultrafast laser oscillators, ultrashort pulse amplifiers, and many other nonlinear optical dynamics. Therefore, dispersion measurement is crucial for device characterization, system design and nonlinear dynamics investigation therein. In this work, we demonstrate a versatile approach, i.e., Kalman filtering-aided white-light interferometry, for group delay dispersion (GDD) characterization. Extended Kalman filter is adopted to track the cosine-like interferogram, and to eliminate the unintended bias and the envelope, providing a nearly ideal phase retrieval and GDD estimation. The measurement range could span from tens of fs2 to tens of ps2, with an uncertainty of about 0.1%, enabling precise GDD measurement for diverse optical components, ranging from a millimeter-thick glass slide to highly dispersive chirped fiber Bragg gratings. Benefited by the simplicity, convenient setup, and easy operation as well as relatively low cost, this approach would help photonic device characterization, dispersion management and nonlinear dynamics investigation in the laboratory and work plant.

A Review on Plant Tissue Culture

Plant tissue and cell culture involve the aseptic cultivation and propagation of plant cells, tissues, and organs in a meticulously controlled laboratory setting. This innovative method harnesses the potential of nutrient-rich media to efficiently replicate plant cells on a large scale, resulting in the rapid production of mature and disease-free plants. The cornerstone of commercial technology in this field is micropropagation, a process that achieves swift proliferation from minute plant cuttings, axillary buds, and, to a limited extent, from somatic embryos and cell clumps in suspension cultures and bioreactors. Micropropagation, a pivotal aspect of plant tissue and cell culture, holds immense value in generating high-quality and consistent planting materials. These materials find applications across diverse fields, including molecular genetic engineering, plant breeding, horticulture production, and environmental preservation. The process of micropopagation unfolds through several distinct stages, including propagation, subculture of explants for proliferation, shooting and rooting, and hardening. These stages collectively form a universal framework for large-scale multiplication of plants. This technique plays a critical role in overcoming the limitations of traditional plant propagation methods. By allowing for controlled and accelerated growth in a laboratory environment, plant tissue and cell culture contribute to the efficient production of disease-resistant and genetically uniform plant materials. This not only supports advancements in various scientific disciplines but also addresses practical needs in agriculture, horticulture, and environmental conservation. The continuous refinement and application of plant tissue and cell culture methods underscore their significance in meeting the growing demands for sustainable and high-quality plant materials across diverse sectors.

Технология производства защитных антикоррозионных материалов из жиросодержащих отходов

The processing of fat-containing waste from meat-packing enterprises that cannot be landfilled is an urgent problem. The authors developed the technology of processing fat-containing waste in a chemical reactor in one step, without dividing the technology into two steps: triglyceride hydrolysis and fatty acid amide production. The technology for processing fat-containing waste was tested in a laboratory plant. The target product obtained was analyzed using IR spectroscopy. As a result, the structural formula was clarified, functional groups were determined, and chemical intermediates in the synthesis process were identified. The proportions of the reagents were determined: technical fat in the amount of 65.3‑72.4 wt%, monoethanolamine - 14.5‑17.0 wt% and boric acid - up to 100 wt% of the mixture. The reaction time was 1.5 h. The protective effect was selected as a quality control indicator of the surfactant obtained. Solvent selection was carried out for producing anticorrosive materials. I‑20 industrial oil, diesel fuel and SN‑150 oil were selected as solvents. Electrochemical studies carried out on the AVTOLAB PGSTAT302N potentiostat-galvanostat made it possible to determine the mechanism of action of the surfactant as a corrosion inhibitor. Its optimum concentration was found to be 15%, with a protective efficiency of 99.33%. To increase the protective anticorrosive efficiency of the surfactant, the tests were carried out in a humidity chamber for 60 days on St3 steel plates. The test results showed high efficiency of the surfactant used as a corrosion inhibitor, with the Z value of more than 90%.

Photocatalytic methanol oxidation to formaldehyde in a continuous laboratory plant over Aeroxide P25

We here report the photochemical synthesis of formaldehyde by gas phase methanol oxidation with oxygen in a sophisticated continuous laboratory setup.

Grain Seed Treatment by a Low-Frequency Electromagnetic Field

The paper notes the potential for enhancing seed productivity through exposure to an electromagnetic field. It touches upon both theoretical and practical considerations for developing an experimental laboratory plant for pre-sowing seed treatment using a low-frequency electromagnetic field and aiming to achieve a magnetobiological effect. (Research purpose) The study aims to investigate the influence of low-frequency electromagnetic field on the composition and properties of grain seeds. (Materials and methods) Standard emitters, characterized by a magnetic induction variability of 3-75 millitesla and a radiation frequency of 1-100 Hertz, were employed to ensure the necessary treatment parameters for a batch of seeds. (Results and discussion) The study investigates the impact of magnetization on the germination process, germination energy, and the physical and chemical properties of seeds subjected to different parameters of a low-frequency electromagnetic field (ranging from 3 to 75 millitesla in magnetic induction and a radiation frequency of 10-16 Hertz).The quality of the effect was analyzed with respect to the installation parameters and seed mass, ranging from 10 to 500 grams, across various cereal crops. Subsequently, specific modes were identified, and a functional irradiation program was devised to ensure a highly effective impact on seeds, optimizing their germination, sprouting and development. (Conclusions) The impact of a low-frequency electromagnetic field on the composition and properties of grain has been studied. An experimental laboratory plant with sources of a low-frequency electromagnetic field has been developed and irradiation modes have been justified. The pre-sowing treatment of an industrial batch, the magnetobiological effect is contingent upon several factors, including the crop type and variety, activation of a biological complex in a cell that initiates the development of a seedling, seed quality and moisture content, the presence of microorganisms on the surface, pathogenic ones included, and the characteristics of irradiation sources and energy parameters. The parameters of the low-frequency electromagnetic field were determined as follows: magnetic induction ranging from 3 to 75 millitesla, a frequency 10-16 Hertz, and seed weight varying from 10 to 500 grams. These identified parameters will be applied in the development of an industrial unit designed for the preparation of seed material and enhancement of its sowing properties.

A new approach for eliminating off-flavors from RAS fish, as part of the normal grow-out period

A new concept is presented for eliminating off-flavor from cold-water RAS-grown fish, while feeding, and as a part of the normal grow-out period. The technology is based on disconnecting the nitrification biofilter, and instead passing the water through an electrolysis system, which both oxidizes the ammonia and disinfects the water, while also removing the off-flavor compounds from the water, which thereby results in the purging of the fish. The purging period was expected to last up to 2 weeks and the fish are fed throughout it. Laboratory and pilot plant experiments were performed to prove the new concept. Lab experiments included quantification of the removal of MIB and geosmin by electrooxidation and stripping, together and separately, in the presence and absence of organic matter. A pilot plant experiment was performed using Rainbow trout to determine the rate at which the off-flavor compounds were removed from the water and the fish flesh (both skin and muscle were tested). The results show that the treatment process eliminated off-flavors in the water after ∼7 days and that the fish were below taste and odor threshold for geosmin and MIB after a maximum of 11 days. Detachment from the biofilter and the fact that the water was vigorously disinfected during the electrooxidation step guaranteed that no further off-flavor compounds would be generated during the operation. Aquacultural-management assessment indicates that RAS farms can increase both their annual production and their income by more than 10%, by implementing the suggested concept as part of the grow-out period.

Experimental study of wastewater micropollutant removal by solar photo‐Fenton using a virtual lab

AbstractNew technologies are providing opportunities for the design of novel educational methodologies. Virtual laboratories can simulate real conditions, thus reducing the workload of teachers and students, and removing the costs associated with laboratory or pilot plant tests. The present work describes the creation of a virtual and interactive lab using the Easy JavaScript Simulation (EJsS) 6.0 tool. The solar photo‐Fenton process for the degradation of the Acetamiprid pesticide (ACTM) as a micropollutant model in wastewater was implemented in discontinuous mode in a stirred tank reactor (STR) and in continuous mode in a raceway pond reactor (RPR). The objective was to achieve a virtual tool that would allow easy, intuitive connection to different devices (laptops, tablets, and smartphones), simplifying the difficulties related to teaching technology. This contribution details the design and implementation process of the model for its use as support material for theoretical classes. The virtual tool presented in this article is an example of how the EJsS platform can be used to teach dynamics systems in chemical engineering.

Increasing the Production of Electrical and Thermal Energy in Biogas Plants through the Optimal Addition of Agricultural Waste

The aim of the work is to increase the yield of biogas, and, accordingly, the generation of electrical and thermal energy in biogas plants by optimally adding agricultural production waste to cattle manure. To achieve the goals set, the following tasks were solved: the yield of biogas from cattle manure was determined with the addition of various volumes of organic waste during periodic loading of the digester; on the basis of the experimental data obtained, the unimodality interval was found for the maximum yield of biogas during the fermentation of cattle pus with the addition of organic waste; Using the dichotomy method, the optimal dosage volume of various types of organic wastes into the substrate for quasi-continuous loading of the digester was calculated. Experimental studies were carried out on a biogas laboratory plant with a useful volume of 30 liters in a mesophilic mode. The most significant result is the determination, using the dichotomy method, of the optimal volumes of organate, starch, flour, whey, wastewater from wineries, crude glycerin, fuse, soap stock and molasses to manure pus to increase the biogas yield. As a result of the research, the optimal dosage formulation of various types of organic waste was substantiated to intensify the fermentation of cattle manure. The significance of the research results lies in the fact that with the optimal addition of these cosubstrates to the digester using special dispensers, the biogas yield increases from 1.4 l/h. kg of dry organic matter and above.

Functional Response and Intraspecific Competition of Three Ladybird Species Feeding on Aphids on Goji Berry Plants in Laboratory and Semi-Field Conditions.

The aphid, Aphis gossypii Glover, is identified as a significant pest that causes severe damage to goji berries in China. To analyze the ladybird consumption of aphids, the functional responses of three ladybird species, Harmonia axyridis, Coccinella septempunctata, and Hippodamia variegata, and intraspecific competition among ladybird individuals were evaluated under laboratory conditions. Moreover, the practical impact of ladybirds on aphid population reduction was investigated in semi-field conditions. We found that all adult ladybirds of the three species exhibited a type II functional response toward aphids. According to Holling's disc equation, H. axyridis exhibited the highest searching efficiency (a = 0.79), while C. septempunctata had the shortest handling time (Th = 5.07 min) among the three ladybird species studied. Additionally, intraspecific competition had a greater impact on H. variegata (m = 0.41) compared to the other two ladybird species. The semi-field study demonstrated that H. axyridis (83.9% reduction) and C. septempunctata (78.7% reduction) exhibited higher efficacy in reducing aphid populations compared to H. variegata (27.3% reduction). This study suggests that H. axyridis and C. septempunctata exhibit potential as effective biological control agents against aphids on goji berry plants and highlights the importance of considering intraspecific competition. However, the results obtained from laboratory and semi-field studies cannot be directly extrapolated to field conditions due to the simplification of these experimental systems. Future field studies are crucial in ensuring the effective implementation of a biological control program.

Enzymatic glycerolysis for the conversion of plant oils into animal fat mimetics

Substituting animal-based fats with plant-based fats of similar stability and functionality has always posed a significant challenge for the food industry. Enzymatic glycerolysis products are systems formed by converting native triacylglycerols in liquid oils into monoacylglycerols and diacylglycerols, mainly studied in the last few years for their unique structural ability. This study aims to modify and scale up the glycerolysis process of different plant oils, e.g., shea olein, palm olein, tigernut, peanut, cottonseed, and rice bran oils, with the goal of producing animal fat mimetics. The reactions were conducted at 65 °C, with a plant oil:glycerol molar ratio of 1:1, and without the addition of water, using a lab-scale reactor to convert up to 2 kg of oil into solid fat. Product characteristics were comparable at both laboratory and pilot plant scales, supporting the commercial viability of the process. Oil systems containing higher levels of both saturated and monounsaturated fatty acids, such as shea olein and palm olein, displayed higher solid fat content at elevated temperatures and broader melting profiles with significantly higher melting points. Comparison of the thermal softening behavior and mechanical properties of these systems with those of pork, beef, and lamb fat showed their high potential to replace adipose fat in the new generation of plant-based meat analogs.

Production of chickpea protein hydrolysate at laboratory and pilot plant scales: Optimization using principal component analysis based on antioxidant activities

Chickpeas are a nutrient-rich source with optimal and high essential amino acid score. To evaluate its potential as a functional food ingredient, 36 chickpea protein hydrolysates were produced at the lab-scale using food-grade enzymes. Parameters including yields, protein content, hydrolysis degree, and antioxidant activities were employed to identify the most favourable conditions for scaling up production to a pilot plant level using a principal component analysis. The selected hydrolysate demonstrated commendable traits: a substantial content of essential amino acids and proteins at 67.71%, notable protein (73.12%) and weight (72.00%) yields, coupled with exceptional solubility exceeding 80%, and a noteworthy digestibility of 89.50%. Upon transition to pilot plant proportions, the hydrolysate retained its attenuated protein profile while exhibiting heightened antioxidant activities. Derived chickpea protein hydrolysates offer promise for innovative foods applications, impacting health and chronic disease prevention.