Laboratory Light Research Articles

Co-administration of either curcumin or resveratrol with cisplatin treatment decreases hepatotoxicity in rats via anti-inflammatory and oxidative stress-apoptotic pathways.

Cisplatin (CIS) is a broad-spectrum anticancer drug, with cytotoxic effects on either malignant or normal cells. We aimed to evaluate the hepatotoxicity in rats caused by CIS and its amelioration by the co-administration of either curcumin or resveratrol. Forty adult male rats divided into four equal groups: (control group): rats were given a saline solution (0.9%) once intraperitoneally, daily for the next 28 days; (cisplatin group): rats were given a daily oral dose of saline solution (0.9%) for 28 days after receiving a single dose of cisplatin (3.3 mg/kg) intraperitoneally for three successive days; (CIS plus curcumin/resveratrol groups): rats received the same previous dose of cisplatin (3.3 mg/kg) daily for three successive days followed by oral administration of either curcumin/resveratrol solution at a dose of (20 mg/kg) or (10 mg/kg) consequently daily for 28 days. Different laboratory tests (ALT, AST, ALP, bilirubin, oxidative stress markers) and light microscopic investigations were done. Administration of CIS resulted in hepatotoxicity in the form of increased liver enzymes, oxidative stress markers; degenerative and apoptotic changes, the co-administration of CIS with either curcumin or resveratrol improved hepatotoxicity through improved microscopic structural changes, reduction in liver enzymes activity, decreased oxidative stress markers, improved degenerative, and apoptotic changes in liver tissues. Co-administration of either curcumin or resveratrol with cisplatin treatment could ameliorate hepatotoxicity caused by cisplatin in rats via anti-inflammatory and oxidative stress-apoptotic pathways.

AUTOMATIC LABORATORY LIGHTING SYSTEM WITH AN INFRARED BASE

The "Infrared-Based Automatic Laboratory Lighting System" represents a novel way to improve energy efficiency and stoner comfort in lab environments. Conventional laboratory lighting systems often warrant rigidity and efficacy, leading to unnecessary energy usage and inadequate lighting. In order to overcome these obstacles, this design incorporates a smart lighting control system that uses infrared (IR) detectors to identify the presence of mortals inside the laboratory. The primary operation of the system entails placing IR detectors in strategic locations across the lab space. These detectors monitor individuals continuously and relay the data they find to a central control unit that is based on a microcontroller platform. The control unit initiates the activation or adaptation of lighting institutions in the vicinity of detected movement upon detection of a mortal presence. This guarantees that the lighting is optimized for continuous conditioning, providing suitable lighting while reducing energy waste during periods of unoccupied occupancy. Crucial features of the proposed system include real-time responsiveness and rigidity to varying residency patterns. The integration of IR detectors with the microcontroller-grounded control unit enables a nippy and accurate discovery of mortal presence, allowing the system to acclimate lighting situations instantly as individuals enter or leave the laboratory space. Additionally, the system can be configured to generate lighting configurations based on particular circumstances, including ambient lighting for common spaces or task lighting for lab workstations. Apart from enhancing energy efficiency, the method places emphasis on the safety and experience of stoners. The technology lowers the risk of accidents or crimes related to dim illumination by providing appropriate and safe lighting conditions that are tailored to the functional needs of the laboratory. Similarly, the automation of lighting control eliminates the need for handcrafted solutions, allowing laboratory workers to focus on research or experimentation. All things considered, the "Infrared-Based Automatic Laboratory Lighting System" provides stoner-friendly and environmentally friendly outcomes to improve lighting operations in laboratory settings. By promoting energy conservation, perfecting the stoner experience, and ensuring safety, this system contributes to the advancement of effective and sustainable practices in laboratory operations. Keywords: LED (light emitting diode), microcontroller, Internet of Things, sensors, relay mechanism, and LCD (liquid crystal display).

Photovoltaic passive cooling via water vapor sorption-evaporation by hydrogel

Photovoltaic (PV) power generation, which converts sunlight into electricity, stands as a pivotal mode of solar energy utilization. The thermal effect poses a significant challenge for all types of PV panels under real operating conditions, as it diminishes both the photovoltaic conversion efficiency and the lifespan of the PV panels. This paper presents a novel passive cooling approach for silicon-based photovoltaic panels, employing night-time hygroscopic hydrogel adsorption, daytime desorption, and subsequent water evaporation for cooling. Both laboratory and outdoor experiments were conducted to validate this strategy. Experimental results reveal that under simulated laboratory light of 1 kW m−2, the hydrogel cooling layer delivers an average cooling power of 288.2 W m−2. It lowers the PV panel temperature by 9.9 °C and enhances both the maximum power and efficiency at equilibrium by 5.92% and 5.93%, respectively. Outdoor experiments in Beijing, China, during summer demonstrated that this method reduced the average daytime temperature of the PV plate by 7.1 °C and increased the average maximum power by 5.21%. The hydrogel layer's high hygroscopicity enables it to support passive cooling effectively all day. Passive cooling strategies for PV panels, leveraging the atmospheric water harvesting cycle, face fewer application constraints. They offer the potential to increase power generation and reduce CO2 emissions from PV plants both now and in the future.

Analisis Pemeriksaan Bilirubin Total Berdasarkan Variasi Waktu Paparan Cahaya Lampu Pada Spesimen

Pre-exam stage one of the most important stages in a laboratory examination which includes patient preparation, specimen reception, specimen processing, identification of specimens and preparation of reagents to be used. Specimens that are not immediately examined can cause the results of the total bilirubin examination to become unstable due to exposure to light which affects the properties of the bilirubin so that the concentration of bilirubin levels in the serum decreases. The purpose of this study was to determine the results of total bilirubin examination based on variations in exposure time to light on the specimens. This research isstudy true experimentby designrandomized pretest-posttest control groupwith 3 treatments, namely exposure to laboratory light on serum specimens for total bilirubin examination (2, 4, and 24 hours). This research was conducted in the hematology and clinical chemistry laboratories, medical laboratory technology department at the East Kalimantan Ministry of Health Polytechnic with a total sample of 40 people. The population in this study were students majoring in medical laboratory technology at the East Kalimantan Ministry of Health Polytechnic. The measurement method used in this research is methodEndPointData analysis used isOne Way ANOVA. The results obtained were clinically and statistically different in the ANOVA test which showed that exposure to light at various times of 2, 4, and 24 hours had a significant effect.

Modulating the Reaction Configuration by Breaking the Structural Symmetry of Active Sites for Efficient Photocatalytic Reduction of Low-concentration CO2.

Photocatalytic conversion of low-concentration CO2 is considered as a promising way to simultaneously mitigate the environmental and energy issues. However, the weak CO2 adsorption and tough CO2 activation process seriously compromise the CO production, due to the chemical inertness of CO2 molecule and the formed fragile metal-C/O bond. Herein, we designed and fabricated oxygen vacancy contained Co3O4 hollow nanoparticles on ordered macroporous N-doped carbon framework (Vo-HCo3O4/OMNC) towards photoreduction of low-concentration CO2. In-situ spectra and ab initio molecular dynamics simulations reveal that the constructed oxygen vacancy is able to break the local structural symmetry of Co-O-Co sites. The formation of asymmetric active site switches the CO2 configuration from a single-site linear model to a multiple-sites bending one with a highly stable configuration, enhancing the binding and structural polarization of CO2 molecules. As a result, Vo-HCo3O4/OMNC shows unprecedent activity in the photocatalytic conversion of low-concentration CO2 (10% CO2/Ar) under laboratory light source or even natural sunlight, affording a syngas yield of 337.8 or 95.2 mmol g-1 h-1, respectively, with an apparent quantum yield up to 4.2%.

Modulating the Reaction Configuration by Breaking the Structural Symmetry of Active Sites for Efficient Photocatalytic Reduction of Low‐concentration CO2

AbstractPhotocatalytic conversion of low‐concentration CO2 is considered as a promising way to simultaneously mitigate the environmental and energy issues. However, the weak CO2 adsorption and tough CO2 activation process seriously compromise the CO production, due to the chemical inertness of CO2 molecule and the formed fragile metal‐C/O bond. Herein, we designed and fabricated oxygen vacancy contained Co3O4 hollow nanoparticles on ordered macroporous N‐doped carbon framework (Vo−HCo3O4/OMNC) towards photoreduction of low‐concentration CO2. In situ spectra and ab initio molecular dynamics simulations reveal that the constructed oxygen vacancy is able to break the local structural symmetry of Co−O−Co sites. The formation of asymmetric active site switches the CO2 configuration from a single‐site linear model to a multiple‐sites bending one with a highly stable configuration, enhancing the binding and structural polarization of CO2 molecules. As a result, Vo−HCo3O4/OMNC shows unprecedent activity in the photocatalytic conversion of low‐concentration CO2 (10 % CO2/Ar) under laboratory light source or even natural sunlight, affording a syngas yield of 337.8 or 95.2 mmol g−1 h−1, respectively, with an apparent quantum yield up to 4.2 %.

Photobleaching of pink photoluminescence in bandicoot fur

ABSTRACT Bright pink-orange-red photoluminescent (fluorescent and/or phosphorescent) fur is being found in an increasing number and diversity of mammal species. With the molecules causing these colours of photoluminescent emission suspected to be mostly photosensitive porphyrins, degradation from light exposure is an unquantified contributor to false negatives in museum-based surveys. I tested the resistance of pink photoluminescent bandicoot, Peramelidae, fur to exposure to natural sunlight and artificial laboratory lighting. Photoluminescence underwent visibly noticeable photobleaching in two minutes of direct sun exposure, or a few hours when exposed to indoor lighting. The fleeting nature of porphyrins means that an accurate representation of pink-orange-red photoluminescence should not be expected in specimens that have been exposed to light, whether in life, post-mortem, during taxidermy or on display.

Morphological and Functional Changes of Pituitary GH and PRL Cells Following Prolonged Exposure of Female Rats to Constant Light

Abstract Light regulates numerous physiological functions including secretion of different hormones. Our aim was to determine morphological and functional changes of the pituitary growth hormone (GH) and prolactin (PRL) producing cells in female rats exposed to constant light regime from the peripubertal to adult period of life. Starting from the thirtieth postnatal day, female Wistar rats were exposed to constant light (600 lx) for the following 95 days. Controls were maintained under the regular laboratory lighting conditions. The GH and PRL cells were immunohistochemically visualized. Changes in cell volumes and volume densities were evaluated by stereology. Concentrations of PRL and GH in circulation were also determined. We detected significant decrease of the GH cell volume and volume density, followed by reduced the GH blood concentration in comparison to the controls. In contrast, PRL cells were larger in size and their volume density was significantly increased when compared to the controls. Accordingly, PRL concentration was elevated. It can be concluded that exposure of female rats to constant light regime, from peripubertal to adult period of life, causes inhibition of the pituitary GH and stimulation of PRL cells.

Influence of the Human Field of View on Visual and Non-Visual Quantities in Indoor Environments.

The visual and non-visual effectiveness of light is often determined by measuring the spectrally weighed irradiance on the corneal plane. This is typically achieved using spectral irradiance or illuminance measurements, captured in a hemispheric (2π) geometry with a diffuser. However, the human binocular field of view (FOV) is not a perfect hemisphere, as it is occluded both upward and downward. Previous research on FOV-restricted measurements is limited, leaving the error from using hemispheric measurements for non-visual quantities undefined. In our study, we tackled this issue by designing and 3D printing FOV occlusions as attachments to spectral measurement devices. We took measurements with and without the occlusion in various laboratory (light from different directions) and real-world lighting situations (light typically from above). Our findings reveal a reduction of visual and melanopic values due to the FOV occlusion. These ranged from negligible to more than 60% in realistic scenarios. Interestingly, the reduction was consistent for both visual and melanopic parameters, as the distribution of light in the FOV was generally spectrally homogeneous. An exception occurred in a specific artificial laboratory situation, where the melanopic daylight (D65) efficacy ratio changed by more than a factor of 2 solely because of the FOV occlusion. Additionally, we observed that head orientation had a marked effect on all quantities measured. In conclusion, our results highlight the potential for substantial errors when solely relying on vertical, hemispheric measurements in experiments and non-visual lighting design projects. We encourage the (additional) use of FOV occlusion in eye-level measurements for typical viewing directions, and we are providing open-source 3D-print files to facilitate this practice.

Comparison of automated video tracking systems in the open field test: ANY-Maze versus EthoVision XT

BackgroundANY-Maze and EthoVision XT are two commonly used automated animal tracking systems employed to produce reliable and consistent results in behavioural paradigms. Data obtained with both tracking systems have presented differences, particularly when varying laboratory lighting conditions and contrasts of mice coat colour against the arena background in both water maze and tunnel maze. MethodIn this study, two fluorescent lighting conditions (58 and 295 lux), local to our laboratory, and different coat-coloured mouse lines (C57BL/6 J - black; CD1 - agouti; C3H/HeN - white) were used to compare reproducibility in measures of tracking systems (ANY-Maze versus EthoVision) in the open field test. ResultsDifferences between systems were reliant on the contrasts between coat and background colours. Surprisingly, black animals presented the greatest differences in read-outs between tracking systems, regardless of lighting conditions. Data from both video observation tools differed mainly in exploration-related parameters (distance travelled), but less in more static proxies (time in thigmotaxis zone). Overall, EthoVision XT returned higher values for most parameters analysed relative to ANY-Maze. More inconsistencies in recording and analysis can be expected from other video recording systems. ConclusionData analysis software provides an additional source of variation in need of consideration when reproducibility in behavioural neuroscience is required.

Effects of herbicide butachlor application on the growth of periphytic biofilms and nitrogen loss in paddy systems

The application of butachlor as an herbicide in paddy fields is widely practiced, aiming to increase rice yield by directly or indirectly influencing the paddy environment. Periphytic biofilms, which form at the soil-water interface in paddy fields, are complex bioaggregates that play an important role in nitrogen (N) cycling. The objective of this study was to investigate the effect of butachlor on periphytic biofilm growth and N cycling under both light and dark conditions in the laboratory. The results revealed that butachlor application hindered the growth of periphytic biofilms and led to the dominance of Cyanobacteria as the primary prokaryotes, while inhibiting the development of eukaryotic Trebouxiophyceae. Furthermore, the application of butachlor reduced the richness and diversity of prokaryotes, but increased those of eukaryotes in periphytic biofilms. The light treatments exhibited higher total N loss because light favored periphytic biofilm growth and enhanced ammonium (NH4+) assimilation and nitrification. Additionally, butachlor application resulted in the increased retention of NH4+-N and nitrate (NO3-)-N and an increase in N loss via denitrification. The abundances of functional genes encoding enzymes such as ammonia monooxygenase, nitrite reductase, and nitrous oxide reductase were increased by butachlor application, favoring nitrification and denitrification processes. Overall, the results suggest that butachlor application leads to an increase in total N loss mainly through denitrification in paddy systems, particularly in the presence of periphytic biofilms. Thus, the results may provide valuable insights into the changes in periphytic biofilm growth and N cycling induced by butachlor, and future studies can further explore the potential implications of these changes in paddy soils.

Energy Efficiency and Feasibility Analysis of Solar Power Generation Using Hybrid System of an Educational Institution in Malaysia

Energy is one of the basic inputs and driving forces for economic and social development modernization. Sustainable energy supply is one of the major challenges in the modern world. Sustainable energy supply can be obtained through alternative energy sources, and efficient energy use is a high priority to optimize the environmental impact. This paper investigates the potential for energy savings involving five end-load equipment in academic institutions. The saving potential is obtained through energy efficiency analysis and feasibility analysis of the solar power generation systems. An energy audit is conducted on end-use consuming equipment (i.e., fans, lights, air conditioning, ICT equipment, etc.) in these five end-load sectors to find out the major energy-consuming equipment, energy consumption, and potential energy savings. The analysis of energy consumption per equipment helps determine the appropriate type of equipment to be upgraded and replaced for energy saving. The feasibility of rooftop solar power generation has been also analysed to integrate into the energy end load. The research findings confirm that laboratory equipment, lighting, and air conditioning are vital to energy consumption in academic buildings. The energy savings, bill savings, and carbon emissions reductions have been estimated based on integrating rooftop solar power generation. The feasibility analysis of onsite energy generation using a hybrid solar system found that the total energy-saving potential and bill savings within five years are 311,131 kWh and RM 113,563, respectively. This value refers to comprehensive energy-saving and bill for all academic buildings involved in this research.

Effect of Applying Strong Column Weak Beam to the Laboratory’s Artificial Lighting System

Lighting is one of the crucial experiment factors in the laboratory. Laboratory’s lighting system that follows the Indonesian National Standard (SNI) can improve visual comfort during experiments. This research aims to determine the effect of strong column weak beam (SCWB) on illuminance in the laboratory according to SNI 6197:2011. The results were expected to provide recommendations for the implementation of SCWB in the laboratory so that the laboratory lighting system was more optimal. Digital Electronics Laboratory, E8 Building, Universitas Negeri Semarang had dimensions of 12m x 7m x 4m. The measurement of illuminance used general measurement and task measurements according to SNI 7062-2019. Illuminance data retrieval was carried out in two ways, namely observation and simulation. Measurement of laboratory’s illuminance by observation has been carried out for 10 days with repetition 10 times. The simulation of illuminance measurement used Dialux 10. Based on the results of observations and simulations, it can be seen that the Digital Electronics Laboratory did not follow SNI 6197:2011 with an illuminance of 31.3 – 137 lux. The simulation used the modifying lamp layout and applying TL LED (Philips Master LEDTube HO) so that the illuminance distribution of the Digital Electronics Laboratory followed SNI 6197: 2011 of 500 – 769 lux. The simulation results of the SCWB dimension variation showed that the recommended thickness of the SCWB was between 10 cm – 30 cm to obtain a more optimal illuminance.

Slowing laser light in the undergraduate laboratory

An experiment involving electromagnetically induced transparency gives students the opportunity to explore quantum applications of lasers.

Recruitment of Melaleuca quinquenervia (Myrtaceae) in the fringing forests of the Myall Lakes, NSW, Australia

Context Melaleuca quinquenervia is a widespread, keystone species of eastern Australian coastal forested wetlands. Populations of M. quinquenervia in the fringes of Myall Lakes, New South Wales, Australia exhibit low numbers of juveniles, infrequent recruitment and a dominance of mature individuals, raising concerns about stand replacement from conservation managers. In contrast, M. quinquenervia often recruits in large numbers and is highly invasive in wetlands around the world. Aims To explore factors that limit recruitment of M. quinquenervia within its native range. Methods Field deployed seed traps; soil-core sampling; a burial longevity trial and depth of emergence experiment; laboratory temperature, salinity and light germination experiments; a transplantation experiment; monitoring of field germination; and a litter/leachate experiment. Key results Low seed viability (5–26%) is offset by high canopy seed loads (250 000 to 120 million seeds per tree) released continuously throughout the year with a peak fall in summer (2000 to 5000 viable seeds per m2 per month), coinciding with optimal germination temperatures. Seedling emergence is reduced below 5 mm soil depth, there is a very low soil seedbank reserve, and germination is light-dependant. M. quinquenervia exhibited reduced germination percentage and rate as well as reduced survival of seedlings at salinities higher than 15 dS m−1 EC (electrical conductivity). The most favourable sites for seedling establishment are permanently moist with low salinity, protected from wave action via sedge vegetation, and sheltered from prevailing winds. There is an inhibitory effect of Casuarina leachate on germination. Conclusions Germination in the field is episodic, and tied to specific environmental conditions. Highest losses occur at the seedling establishment phase. Implications Future research avenues and management recommendations are provided.

Green synthesis silver nanoparticles Bougainvillea glabra Choisy/LED light with high catalytic activity in the removal of methylene blue aqueous solution.

In this study, we evaluated, in a pioneering way, the influence of wavelengths from the decomposition of white light on the production and physicochemical properties of silver nanoparticles (AgNPs). Bearing in mind a process of green synthesis, an extract of the bracts of Bougainvillea glabra Choisy (BgC) was used, a species native to tropical and subtropical regions and frequently used in ornamentation, possessing in its photochemical composition, biomolecules capable of acting as reducing agents for convert Ag+ to Ag0. We used light-emitting diodes (LED) to obtain the desired wavelengths (violet, blue, green, yellow, orange, and red) in the test called rainbow, and we evaluated the obtaining of AgNPs compared to white LED light, nature, and absence of light. In the rainbow assay, we obtained a gradual increase in the intensity of the plasmonic band resonance from the red wavelength (0.124 ± 0.067 a.u.) to violet (0.680 ± 0.199 a.u.), indicating a higher reaction yield in obtaining AgNPs. Smaller hydrodynamic sizes (approximately 150nm) at more energetic wavelengths (violet, blue, and green) about less energetic wavelengths (yellow, orange, and red) (approximately 400nm) were obtained. Analysis by SEM microscopy, FTIR spectroscopy, and X-ray diffraction indicates the presence of silver nanoparticles in all LED colors used together with white LED light and Laboratory light (natural light). Due to the high environmental demand to remove pollutants from water sources, including textile dyes, we applied AgNPs/BgC to remove methylene blue (MB) dye from an aqueous solution. A minimum removal percentage greater than 65%, with emphasis on formulations synthesized by the colors of violet LED (84.27 ± 2.65%) and orange LED (85.91 ± 1.95%), was obtained.

Under the influence of light: How light pollution disrupts personality and metabolism in hermit crabs

Anthropogenic disturbances are known to cause significant physiological and behavioural changes in animals and, thus, are the critical focus of numerous studies. Light pollution is an increasingly recognised source of disturbance that has the potential to impact animal physiology and behaviour. Here, we investigate the effect of constant light on a personality trait and metabolic rate in the European hermit crab Pagurus bernhardus. We used Bayesian mixed models to estimate average behavioural change (i.e. sample mean level behavioural plasticity) and between- and within-individual variation in boldness in response to laboratory light. Hermit crabs experiencing constant light were consistently less bold and had a higher metabolic rate than those kept under a standard laboratory light regime (12:12 h light/dark). However, there was no effect of light on individual consistency in behaviour. As boldness is associated with coping with risk, hermit crabs exposed to light pollution at night may experience increased perceived predation risk, adjusting their behaviour to compensate for the increased conspicuousness. However, reduced boldness could lead to lower rates of foraging and this, in combination with elevated metabolic rate, has the potential for a reduction in energy balance.

Metabolic Demands and Kinematics During Level Walking in Darkness With No Vision or With Visual Aid.

Uniformed services commonly perform foot-borne operations at night, while using visual aid in terms of night vision goggles (NVG). During slow-level walking, complete lack of visual input alters kinematics and markedly increases the metabolic demand, whereas the effect on kinematics and energy expenditure of restricting the peripheral visual field by wearing NVG is still unknown. The purpose was to evaluate whether metabolic demands and kinematics during level walking are affected by complete darkness with and without visual aid. Eleven healthy men walked on a treadmill (inclination: +2.3°, velocity: 4 km/h) with full vision in a lighted laboratory (Light), and in complete darkness wearing either a blindfold (Dark), or restricting the visual field to about 40° by wearing monocular (Mono) or binocular (Bino) NVG. Oxygen uptake ($\dot{\text{V}}$O2) was measured to evaluate metabolic demands. Inertial measurement units were used to estimate kinematics, and the outcome was validated by using a motion capture system. Ratings of perceived exertion, discomfort, and mental stress were evaluated after each condition using a Borg ratio scale. Physiologic and kinematic variables were evaluated using repeated measures analysis of variance (ANOVA), whereas ratings were evaluated using non-parametric Friedman ANOVA. $\dot{\text{V}}$ O2 was 20% higher in the Dark (1.2 ± 0.2 L/min) than the Light (1.0 ± 0.2 L/min) condition. Nominally, $\dot{\text{V}}$O2 in the Mono (1.1 ± 0.2 L/min) and Bino (1.1 ± 0.2 L/min) conditions fell in between those in the Light and Dark conditions but was not statistically different from either the Light or the Dark condition. Step length was shorter in the Dark (-9%, 1.22 ± 0.16 m) and Mono (-6%, 1.27 ± 0.09 m) conditions than in the Light condition (1.35 ± 0.11 m), whereas the Bino (1.28 ± 0.08 m) condition was not statistically different from either the Light or the Dark condition. The three conditions with no or limited vision were perceived more physically demanding, more uncomfortable, and more mentally stressful than the Light condition, and the Dark condition was perceived more mentally stressful than both NVG conditions. The study confirms that complete lack of visual cues markedly reduces the mechanical efficiency during level walking, even under obstacle-free and highly predictable conditions. That $\dot{\text{V}}$O2 and step length values for the NVG conditions fell in between those of the Light and Dark conditions suggest that both foveal and peripheral vision may play important roles in optimizing the mechanical efficiency during level walking.

Simultaneous Determination of Retinols and Tocols in Egg and Milk Products Based on RP-HPLC Linked with Fluorescent and Photodiode Array

In this paper, we built a method of verifying the 4 retinols of vitamin A and 8 tocopherol isomers of vitamin E in the food industry based on RP-HPLC-PDA-FLR. The effect of laboratory light conditions on the target components was considered for the first time, and it was found that the loss rate of the target components was the smallest in the case of a white laboratory bench with red or LED light in the dark room. There was no finding of extraction recoveries of the target components with a significant difference under different saponification conditions. Meanwhile, it was found that using ethyl acetate/n-hexane mixed solvent as the extraction solvent could ensure the effective extraction of the target components. Finally, baseline separation of 12 components was achieved within 45 min using the C30 column. With the help of methodological verification, we found that the recovery rate ranged from 76.45% to 93.52%, and RSD was between 0.19% and 12.99%; the Limit of Detection minimum value was 0.01 mg/100 g and the Limit of Quantitation minimum value was 0.03 mg/100 g. The detection method was successfully applied to the distribution detection of 4 kinds of retinols and 8 kinds of vitamin E in egg and dairy products and provided technical support for the accurate nutritional evaluation of vitamin A and vitamin E.

ZnO:Fe3O4Nanoparticles Produced by Cold Plasma: Synthesis, Characterization and Anti-Microbial Activity

The cold atmospheric plasma in liquid (CAPL) technique was carried out in DI water as the solution to create Fe3O4-doped ZnO nanoparticles by adjusting the concentration (0.01, 0.02 and 0.03) M at ([Formula: see text][Formula: see text]kV, [Formula: see text][Formula: see text]mA and flow rate = 3[Formula: see text]L/min), while the exposure period was 3[Formula: see text]min. A UV-Vis. spectrophotometer, X-ray diffraction and field emission spectroscopy (FE-SEM) were used. The energy of the bandgap was discovered to be (2.95–2.85) eV. The form and dimensions obtained by FE-SEM have not changed much. All materials were tested against inactivated positive and negative gram bacteria (S. aureaus and E. coli) and showed promising antibacterial capabilities, especially Fe3O4-doped ZnO, which can inactivate bacteria under typical laboratory light.