Introduction: Trichomonas vaginalis and Candida sp is one of the pathogens common vaginal infection in the world. This study aimed to assess the value of diagnostic techniques fungus and Trichomonas vaginalis. Materials and method: Using laboratory direct test, culturing fungi, Trichomonas vaginalis and compared the results of both technique, thereby determining the sensitivity, specifi city of direct test from that culturing. Through culture techniques, identify Candida albicans and Candida non albicans infection rate. Perform ELISA technique to determine the rate of IgG antibodies Trichomonas vaginalis simultaneously using ROC curves assessed the sensitivity and specifi city of ELISA with the ratio of serum diluted 1/50, 1/100 and 1/200. Results: The study on 201 patients with vaginal infections, with 44.77%, infections caused by fungi and / or Trichomonas vaginalis including 2 types mixed infection is 0.99%. In 63 cases of fungal, 12 cases of Candida albicans, accounting for 19.05% ratio, remaining Candida non albicans remaining. With Trichomonas vaginalis, direct technique detected 27 cases, culture technique detected 31 cases. ELISA technique detects antibodies T.vaginalis 77.40% sensitivity, specifi city of 89.00% with a serum dilution 1/100. Conclude: Direct techinique has sensitivity and high specifi city, useful for diagnosis fungal, Trichomonas vaginalis. Elisa technique has sensitivity and high specifi city, useful for diagnosing trichomonas vaginalis, especially in epidemiological study. Key words: vagina, fungus, Candida albicans, Candida non albicans, Trichomonas vaginalis, culture, direct technique, ELISA