Laboratory Car Research Articles

LAW ENFORCEMENT BPOM BANDA ACEH AGAINST CRIMINAL ACTS. FOOD PRODUCTION USES BORAX AND FORMALIN

The need for food production is inseparable from the efforts of consumers who want food to last longer, thus triggering food producers to compete to make the food according to the wishes of consumers in a way that is not justified by laws and regulations, but this method will cause various diseases, even death. BPOM has a basis for law enforcement against criminal acts of food production according to the provisions of Article 6 Paragraph (1) and Article 7 Paragraph (2) of Law Number 8 of 1981 concerning criminal procedural law. The questions in this thesis are how is the law enforcement of food production crimes carried out by BPOM Banda Aceh, and what are the inhibiting and supporting factors for food production crimes. This study uses field research and uses qualitative research methods with an empirical juridical approach and applies two forms of data collection methods, namely interviews and documents. The results of the study showed that law enforcement was carried out by BPOM Banda Aceh, namely based on evidence from testing results, the process was submitting evidence to the prosecutor's office, destroying evidence, imposing administrative sanctions, BPOM obstacle factor was the lack of investigators, investigators did not have much authority in detention, requests from consumers, food producers do it repeatedly, while the supporting factors for BPOM investigators are having a laboratory car, cooperative actors. From the explanation above, the authors can conclude that law enforcement by BPOM investigators is still not optimal and still has obstacles.

Role of Microenvironment in Influencing the Activity of CD19 CAR T Cells in B-Cell Precursor Acute Lymphoblastic Leukemia (B-ALL)

Background: As a therapy based on immune cells, CAR T cells treatment elicits an acute response involving both innate and adaptive immunity which is followed by a process of resolution. A major interest is to identify, within the tumor microenvironment (TME), factors that influence the activity and potency of anti-CD19 CAR T cells. The B-ALL TME provides factors that protect leukemia and, conversely, suppress effector T cells. However, studies on the interaction between the bone marrow (BM) TME and CAR T cells are still limited. We hypothesized that the immunological niche of the BM reacts to CAR T-cell-mediated inflammation by modulating the immune response through the activation of inhibitory pathways and molecules. Methods: We conducted an academic phase I/II study (NCT03389035) with donor-derived anti-CD19 CAR T cells generated with Sleeping Beauty transposon (CARCIK-CD19) in B-ALL patients relapsed after alloHSCT. Hematology laboratory data, CAR T,and CD3+ cell kinetics were analyzed in 43 pediatric and adult B-ALL patients who underwent commercial and investigational anti-CD19 CAR T cell treatment. The scRNA-seq libraries of BM samples were generated using Chromium Single Cell 5′ Reagent Kit of the 10x Genomics (v3.1). Validation was performed in 20 pre-/post- CAR-T matched BM samples by a 30-color flow cytometry panel. Results: Following the peak of expansion, CAR T-cell counts decreased in the third and four-week post-infusion. A similar dynamic of expansion and contraction was observed in CD3+ T cells, total white blood cells (WBC), and monocytes, indicating that a widespread resolution of CAR T-driven inflammation occurs during the first month after infusion. Interestingly, the intensity of the response seems to differ depending on the cell product used. To elucidate the mediators involved in the resolution of CAR T-cell-mediated inflammation, the single-cell transcriptome of patients' BM cells at early time points post-CAR T-cell infusion (1-2 months) was compared to pre-treatment samples at the moment of disease relapse and the infusion products. Eighteen sequencing libraries were analyzed, 6 libraries per patient, for a total of 71407 high-quality cells at an average of 65000 reads per cell. Unbiased clustering of BM cells and infusion products was performed by UMAP embedding. Notably, extensive integration of the dataset coming from the individual patients was observed.The more representative clusters were classified into infusion product, CD4 and CD8 endogenous population, B cells, myeloid cells, pDC, NK, and NK-T cells. We observed profound changes in the composition of BM after CAR T cells infusion compared to pre-treatment samples. Complete disappearance of the B cells associated cluster was observed after treatment. CAR T-cell treatment generated a remodeling of the BM microenvironment, with an increase in the myeloid cells (specifically monocytes), NK, NK-T, and exhausted CD8+ T cell populations. After CAR T-cell infusion, myeloid cells displayed a higher resemblance to myeloid-derived suppressor cells (MDSCs). GeneSet Enrichment Analysis (GSEA) showed significant enrichment in pathways associated with immunosuppression both in the myeloid compartment and in endogenous T cells and in CAR T cells. Of note, the genes involved are also strictly correlated to the generation of terminally exhausted T cells and the emergence of MDSCs. Spectral flow cytometry-based experiments validated our observation of an increase of monocytes, MDSC-like cells, NK, NK-T, and CD8 terminal cells. Modeling intercellular communication using NicheNet suggested the induction of immunosuppressive genes in myeloid cells by both CAR T cells and endogenous T cells. Using the same tool with all niches as the sender and T cells as the receiver, we observed that myeloid cells induced significant signaling in both CAR T cells and endogenous T cells. Conclusions: Through sc-RNAseq and spectral flow cytometry, we have characterized changes in the BM TME after CAR T cell therapy. Specifically, CAR T cells-mediated myeloid activation is associated with pathways of immune dysregulation that may dampen CAR T cell expansion and antagonize the effects of the therapy. Furthermore, these data support the hypothesis that transcriptomic interrogation can infer pathways relevant to the communication between CAR T cells and the TME.

Method for detecting the source of radio interference affecting train radio communication in the band of 2.13 and 2.15 MHz

There are many factors that infl uence the process of organising train movements, quality radio communication being one of the most important. The intelligibility of the dispatcher’s commands to the driver is important in deciding what action to take, and in informing the dispatcher of the train situation on a particular section of the track. Sources of radio interference that have an interfering effect on the quality of the train radio signal are natural and artifi cial interference. The sources are mainly low-frequency interference. One of the constant sources of high-frequency emissions that affect stable operation of train radio communications refers to discharges generated in the high-voltage insulation of power lines, including discharges generated in the insulation of overhead wires. Spark discharges, the source of radio interference, occur both on the surface of the insulator (usually called surface partial discharges (SPD)) and inside the insulation (such discharges are called partial discharges (PD)). In the operation of high voltage insulation, those insulators that contain PD and PPD are called defective insulators. PD and PD diagnostics are carried out twice a year by means of a laboratory car. Discharge registration is performed in the visible and ultraviolet range. There are acoustic methods for registering PD and SPD. It is proposed to register discharges in the electromagnetic frequency range. For prompt detection radio interference, it is recommended to place antennas on the laboratory car, and combine measurements with current diagnostics of high-voltage insulation, that will allow increasing reliability of the results received in the course of diagnostics. To increase the accuracy of radio interference source detection, two antennas are considered to be placed on the laboratory car.

Comparative Analysis of Railway Track Depression Estimation Methods

There are presented the results of implementation and comparative analysis of three various technologies of railway track depression estimation including using a mobile laboratory car, application of accelerometer sensors on rails and simulation using a finite-element method. The main attention is paid to the study of the interdependencies of the measured parameters. It was in studies revealing that railway track depression is better characterized by a parameter of the impact force of the rolling stock on the rail. This parameter depends on a number of factors including the speed of the train, the workload of the cars, the swaying of the cars, the presence of defects in the wheel set, etc. The combination of different research methods for railway track depression estimation makes it possible to expand the possibilities of a comprehensive analysis of the state of the railway track, improve the accuracy of the assessment and provide the possibility of a long-term forecast of its technical condition. This is an important step to ensure transport safety on the railway.

Velocity Errors Associated with Application of Inertial Methods for Detecting Rail Surface Short-Wavelength Irregularities

Introduction. Efficient operation of a system for measuring rail surface short-wavelength irregularities depends on evaluation of the signal length with respect to travelled distances, i.e., the standard maximum defect length and the distance between the wheels of a car bogie. When recording an accelerometer signal with respect to time due to the effect of velocity, the signal length corresponding to these spatially constant distances will vary.Aim. Development of an algorithm for determining rail running surface defects using the data obtained by accelerometers mounted on the axle boxes of a car bogie under their not equidistant spatial record.Materials and methods. The data obtained when passing a laboratory car equipped with a system for measuring short-wavelength irregularities was used. The search and determination of irregularity parameters was carried out by an inertial method. The methods of normalization and correlation analysis were used.Results. An algorithm for determining rail running surface defects based on an inertial method was developed, considering the spatial non-equidistance of the signal. The implemented correlation analysis allows compensation of high-velocity errors when determining the defects. In the considered example, the relative error equaled 0.4 %. Compensation of velocity errors reduces the probability of type I errors in defect determination.Conclusion. The developed algorithm considers velocity errors associated with the application of inertial methods for detecting short-wavelength irregularities. The implementation of correlation analysis reduces the probability of type I errors when determining rail running surface defects.

Improvement of Video Measuring Systems for Electric Traction Network Diagnostics

Purpose. The purpose of the article is system analysis of the state of electric traction networks, as well as methods of complex diagnostics of the contact network from a moving laboratory car to increase the resolution capability of the systems for monitoring the quality of interaction between the contact network and current collectors. Methodology. The problem was solved by theoretical analysis and experimental studies of the current collection parameters, a generalized model of the device for monitoring the wear of the overhead wire and its functional units in order to determine the factors affecting the control error, as well as the development of methods that reduce the specified error. The apparatus of factor analysis, the theory of optoelectronic circuits and methods of statistical information processing were used. Findings. Innovative approaches and qualitatively new diagnostic tools are proposed that allow expanding the functionality of the laboratory cars for testing the contact network for power supply enterprises of electrified railways, industrial and urban electric transport. Hardware and software have been developed to improve the system for measuring the parameters of the overhead wire and other components of the contact network. Originality. The theoretical maximum permissible, from the point of view of the contact network operation, error in monitoring the wear of the overhead wire and other components of the electric traction network has been determined. A method for increasing the resolution capability of a stereo television system and an adaptive lighting system is proposed. It consists in preliminary image transformation and expansion of the dynamic range of image measurement. The ways of introducing a high-speed real-time compression algorithm and using LED backlighting are proposed. Practical value. The quality of the contact network diagnostics in difficult conditions for video surveillance has been improved. A camera with a built-in image compression module without losing its performance is proposed, which allows capturing and transmitting full-frame images to a computing complex for the application of new diagnostic algorithms for contact network components. The modernized video measuring systems for the wear of the overhead wire for monitoring the grounding of the contact network supports are proposed, as well as elements of track facilities located in the visibility zone of specialized cameras, which ensure the operability of the systems at any time of the day at speeds up to 160 km/h. An air curtain subsystem was implemented to protect the cameras.

Detection of delamination between plane layered media using pulse geodars

The relevance of the problem follows from the consideration of the consequences of such serious road pavement defect as delamination. Also, the validity of the problem stems from the fact that the use of modern pulsed GPR provides low-cost monitoring of roads. This becomes possible when obtaining primary GPR data during the movement of the laboratory car with the speed of the transport stream, and, in addition, due to the exclusion of such labour-intensive operations as coring or cutting sections of pavement. The purpose of the work is to analyze various processing methods for ultra-wideband signals of pulsed GPR to study the capabilities of signal processing methods and algorithms to increase the reliability of detection of delamination. Materials and methods. During the investigation of the processing model and experimental data, first of all, modern theoretical methods of signal processing and computer modelling were used. Results. The factors determining the differences and characteristic features of pulsed signals of GPR, which can be used to detect delamination, as a violation of contact between layers, or to detect the presence of thin layers in a multilayer medium, are established and analyzed. Conclusions. The proposed approach to the formulation of the problem, together with the theoretical analysis and the results of numerical modelling, made it possible to justify the possibility of increasing the reliability of the non-destructive quality control of various structures and the effective detection of critical defects using GPR methods.

Simple Discrete-Time Switched H∞ Optimal Control: Application for Lateral Vehicle Control

This paper presents a switched H^ optimal control for a class of discrete-time switched linear systems. All sufficient conditions of the existence of the control law are proved and given in terms of LMI for any switching. Moreover, the proofs are established using an H^ norm and switched Lyapunov functions. Its performances are shown through a steering vehicle control application. In fact, the vehicle models are affected by several parameter variations like longitudinal speed, cornering stiffnesses coefficients. The validation step is conducted using real data acquired by a laboratory car under high lateral loads.

Steering vehicle control and road bank angle estimation: application for diagnosis of vehicle limits in bend

This paper deals with the diagnosis of the critical driving situations. This work is divided into three parts: the first one presents two steering controllers using the sliding mode and the switched H∞ controllers, the second one describes an unknown input sliding mode observer, while the last one presents an approach to the diagnosis of critical driving situations. All existence conditions are established using the Lyapunov approach. Simulations are conducted to highlight the efficiency of the proposed approach using the experimental data recorded by an instrumented Peugeot 307 laboratory car. The diagnosis of the critical driving situations is achieved by the speed extrapolation concept using simultaneously a non–linear model, a steering control and road bank observer. The speed extrapolation concept is used in order to evaluate situations under high dynamic loads on a bend, using stability of the sideslip motion.

Synthesis and characterization of elastomeric polyester coatings for automotive pre-coated metal

An automotive pre-coated metal system (PCM) has been investigated to replace wet coating process, such as pre-treatment, dip coating and spray coating, to address environmental regulations. However, automotive pre-coated metal sheets must have high flexibility and stiffness to overcome harsh conditions such as cutting, press and stamping processes. For these reasons, elastomeric polyester coatings were designed to improve scratch resistance and to impart reflow characteristic for an automotive PCM. The characteristics, curing behavior and viscoelastic property of the resins were measured by 1H NMR, GPC, RPT and DMA. The flexibility was evaluated using a texture analyzer. A nano scratch tester, equipped with an optical microscope was used to measure the scratch resistance of coatings and the scratched surface morphology. An Amtec laboratory car wash test and a sharp knife were used to evaluate the reflow property of the coatings. Change of the damaged surface morphology was measured using a mini-SEM.With increasing 1,4-cyclohexanediol content, the final frequency decreased and the storage modulus and Tg of the coatings increased. This is related to the high stiffness of the cyclohexane structure of 1,4-cyclohexanediol. In terms of scratch resistance, EP-6, one of the formulations examined, has high scratch resistance due to the restoring force of the stiff structure of 1,4-cyclohexanediol. Consequently, the gloss recovery after a heat treatment was increased with increasing the content of cyclohexane structure in the car wash test. In the case of a deep scratch, the reflow property of coating was demonstrated using a heat treatment. Therefore, a cyclohexane structure is a good factor for improving the scratch resistance and reflow property of the polyester coatings.

Observers for vehicle tyre/road forces estimation: experimental validation

The motion of a vehicle is governed by the forces generated between the tyres and the road. Knowledge of these vehicle dynamic variables is important for vehicle control systems that aim to enhance vehicle stability and passenger safety. This study introduces a new estimation process for tyre/road forces. It presents many benefits over the existing state-of-art works, within the dynamic estimation framework. One of these major contributions consists of discussing in detail the vertical and lateral tyre forces at each tyre. The proposed method is based on the dynamic response of a vehicle instrumented with potentially integrated sensors. The estimation process is separated into two principal blocks. The role of the first block is to estimate vertical tyre forces, whereas in the second block two observers are proposed and compared for the estimation of lateral tyre/road forces. The different observers are based on a prediction/estimation Kalman filter. The performance of this concept is tested and compared with real experimental data using a laboratory car. Experimental results show that the proposed approach is a promising technique to provide accurate estimation. Thus, it can be considered as a practical low-cost solution for calculating vertical and lateral tyre/road forces.

Lateral load transfer and normal forces estimation for vehicle safety: experimental test

Knowledge of vehicle dynamics data is important for vehicle control systems that aim to enhance vehicle handling and passenger safety. This study introduces observers that estimate lateral load transfer and wheel–ground contact normal forces, commonly known as vertical forces. The proposed method is based on the dynamic response of a vehicle instrumented with cheap and currently available standard sensors. The estimation process is separated into three blocks: the first block serves to identify the vehicle’s mass, the second block contains a linear observer whose main role is to estimate the roll angle and the one-side lateral transfer load, while in the third block we compare linear and nonlinear models for the estimation of four wheel vertical forces. The different observers are based on a prediction/estimation filter. The performance of this concept is tested and compared with real experimental data acquired using the INRETS-MA (Institut National de Recherche sur les Transports et leur Sécurité – Département Mécanismes d’Accidents) Laboratory car. Experimental results demonstrate the ability of this approach to provide accurate estimation, thus showing its potential as a practical low-cost solution for calculating normal forces.

Evaluation of a sliding mode observer for vehicle sideslip angle

This paper presents a sliding mode observer of vehicle sideslip angle, which is the principal variable relating to the transversal forces at the tire/road interface. The vehicle is first modelled, and the model is subsequently simplified. This study validates the observer using both a validated simulator and real experimental data acquired by the Heudiasyc laboratory car, and also shows the limitations of this method. The observer requires a yaw rate sensor and data about vehicle speed are required in order to estimate sideslip angle. Some properties of the nonlinear observability matrix condition number are discussed, and relations between this variable and observation error, vehicle speed and tire cornering stiffness are presented.

Scratch resistance of automobile clearcoats: Chemistry and characterization on the micro-and nanoscale

The scratch resistance of polymeric clearcoats intended for automobile exterior surfaces was tested with scanning 3D nanoindentation and laboratory car wash simulation (AMTEC). It could be shown by using “real world” samples with comparable scratch resistance that the applied methods were suitable for discriminating even small differences in scratch resistance with high accuracy. In the area of physical characterization of scratch resistance, the results of this investigation demonstrate the ability of microscopic, single-contact testing methods to reproduce the macroscopically experienced results. By using a combination of different scratch methods, insight into the fundamentals, e.g., chemistry, that are responsible for good or bad scratch resistance, is achieved. One key for this goal is the imaging and analytical evaluation of the damage pattern after the indent, which leads to additional results and models presented in this article. It was found that an essential key to obtaining scratch resistant surfaces is a strengthening of rubber-like elasticity as well as minimizing interactions between individual polymeric chains.

DETERMINATION OF PARAMETERS OF THE TRACK CIRCUIT DURING THE MEASURING WITH THE MOBILE LABORATORY

A method is proposed for determination of rail circuits parameters in measurements with the use of automated microprocessor system, installed in a laboratory car. The system allows to check and measure the parameters of current in the rail circuits. The value of primary and secondary parameters of the rail circuits is determined by the data of measurement of code current in rails.

Experimental evaluation of vehicle sideslip angle observers

The lateral behavior of a vehicle is defined by the transversal forces between theroad and the wheels. The sideslip angle is the principal variable for the transversal forces. This paper compares four observers of vehicle sideslip angle. Modeling, model simplification are performed. This study validates observers on experimental data acquired by the Heudiasyc laboratory car. An optical speed sensor “Correvit” is used to acquire the sideslip angle. This paper shows that observers can give good results in spite of constraining assumptions.

Effect of weathering on scratch/mar and mechanical behaviour of clearcoats

The study examines the effect of weathering on mechanical and chemical properties of two 2K (twopack, with and without light stabilisation additives) and one 1K (onepack) clearcoats, concentrating on scratch and mar resistance and acid etch resistance. The clearcoats were investigated before and in the course of accelerated weathering. For determining the mar resistance two different instruments were used: - AMTEC laboratory car wash equipment (rotating wet brush) - Crockmeter Explanations for the changes in clearcoat properties caused by weathering are given by Dynamic-Mechanical Analysis, FT-IR-Spectroscopy, Universal-hardness and surface tension measurements. The clearcoat systems investigated show a clear deterioration of their properties after short periods of accelerated weathering. This effect is more expressed for the clearcoat samples without light stabilizers.

The influence of weathering on scratches and on scratch and mar resistance of automotive coatings

Automotive coatings are expected to retain a good scratch resistance over years of service. On the other hand, scratches once arisen should disappear by reflow or should not become more visible during further use of the car. These expectations are a challenge to the paint manufacturer and are not easy to fulfil as our work will show. Seven car coatings were examined, each with different PU clearcoats. The coatings were treated under artificial weathering conditions before and after the scratch test. Scratching was carried out using laboratory car wash and Nano Scratch techniques. In comparison to other essential coating properties measured, the scratch and mar resistance was mainly affected by pre-weathering. Alterations in reflow behaviour, shape and size of single scratches were observed during post-weathering which included partly acid precipitation.

Automotive coating

Automotive coatings are expected to retain a good scratch resistance over years of service. On the other hand, scratches once arisen should disappear by reflow or should not become more visible during further use of the car. These expectations are a challenge to the paint manufacturer and are not easy to fulfil as our work will show. Seven car coatings were examined, each with different PU clearcoats. The coatings were treated under artificial weathering conditions before and after the scratch test. Scratching was carried out using laboratory car wash and Nano Scratch techniques. In comparison to other essential coating properties measured, the scratch and mar resistance was mainly affected by pre-weathering. Alterations in reflow behaviour, shape and size of single scratches were observed during post-weathering which included partly acid precipitation.

Testing and evaluation of a hybrid III load sensing face

To detect localized facial forces in laboratory car crash conditions, a load sensing face (LSF) was developed. Piezoelectric sensors provide pressure time histories. Design properties and characteristics of the LSF are described, as well as the sensor technique used. Sled tests with a LSF equipped dummy are presented. The purpose was to evaluate the efficiency of the method in registering facial impact magnitude and location with different restraint and seat position combinations. The LSF was also used in drop testing of steering wheels. Various impact velocities and impact points as well as the reapeatability were studied.